Cp4.1LG04g00580 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG04g00580 vs. ExPASy Swiss-Prot
Match: Q5H7P5 (Mannosylglycoprotein endo-beta-mannosidase OS=Lilium longiflorum OX=4690 GN=EBM PE=1 SV=4)

HSP 1 Score: 1459.9 bits (3778), Expect = 0.0e+00
Identity = 683/974 (70.12%), Postives = 801/974 (82.24%), Query Frame = 0

Query: 4   IGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNKVVP 63
           +GK++ L+SGWLAARSTE+EL+G QLTTT PPS G S+PW+EA VPGTVLGTL+KNK+VP
Sbjct: 1   MGKQV-LDSGWLAARSTELELTGVQLTTTRPPS-GTSAPWIEAVVPGTVLGTLLKNKLVP 60

Query: 64  DPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYINGHQK 123
           DPFYGL NE I+DI DSGREYYTFWFF +F+CKLSE+QH+ LNFRAINYSAEVY+NGH++
Sbjct: 61  DPFYGLNNEGILDIYDSGREYYTFWFFKSFECKLSENQHVSLNFRAINYSAEVYLNGHKE 120

Query: 124 VLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAAQYV 183
           +LPKGMFRRHS+D++D++ PDG N+LAVLVHPPDHPG+IP +GGQGGDHEIGKDVA QYV
Sbjct: 121 ILPKGMFRRHSIDITDILHPDGKNMLAVLVHPPDHPGQIPPEGGQGGDHEIGKDVATQYV 180

Query: 184 EGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNKSSW 243
           EGWDWMAPIRDRNTGIWDEVS+  +GPVKI D HLVS+FFD ++R YLH+T+E++NKSSW
Sbjct: 181 EGWDWMAPIRDRNTGIWDEVSLYTSGPVKIADVHLVSSFFDMFRRAYLHSTVELENKSSW 240

Query: 244 VADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGMGKQ 303
            A+CS+ + VTTEL+G+  L+E+ Q+ ++S+P  S IQYT P L+FYKPNLWWPNGMGKQ
Sbjct: 241 RAECSLTILVTTELDGDFNLIEYHQTHELSIPPESVIQYTLPPLFFYKPNLWWPNGMGKQ 300

Query: 304 SLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWILSD 363
           SLYNV I++ V GFG+SDSWN+ FGFR+IES ID ATGGRLFKVNGQ +FIRGGNWILSD
Sbjct: 301 SLYNVEITIAVKGFGDSDSWNNKFGFRQIESAIDEATGGRLFKVNGQRVFIRGGNWILSD 360

Query: 364 GLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITGDVD 423
           GLLRLS+KRY TDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITGD D
Sbjct: 361 GLLRLSKKRYMTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITGDCD 420

Query: 424 GRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKDLEL 483
           GRG+PVSNPNGPLDH LFL CARDT+KLLRNH SLALWVGGNEQ+PP DIN+ALK DL+L
Sbjct: 421 GRGIPVSNPNGPLDHALFLHCARDTIKLLRNHASLALWVGGNEQIPPEDINSALKNDLKL 480

Query: 484 HPHFQTSSENEKWMV--DSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEIQ 543
           HP F+    N   ++  D  SE+EDPSQYLDGTR+Y+QGSMW+GFANGKGDFTDGPYEIQ
Sbjct: 481 HPFFK---HNGVTVIGEDMLSETEDPSQYLDGTRVYIQGSMWEGFANGKGDFTDGPYEIQ 540

Query: 544 YPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPIW 603
            PE+FFKDDFY YGFNPEVGSVG+PVAATIRATMP EGW+IPL K+L  G++EEVPNPIW
Sbjct: 541 NPEDFFKDDFYSYGFNPEVGSVGVPVAATIRATMPPEGWQIPLFKRLSDGFIEEVPNPIW 600

Query: 604 DYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTGF 663
           +YHKYI YSKP KV  QI LYG P +LDDFC KAQL NY+QYRAL+EGW  RMW KYTG 
Sbjct: 601 EYHKYISYSKPGKVHDQIVLYGQPTNLDDFCEKAQLVNYVQYRALLEGWTSRMWTKYTGV 660

Query: 664 LIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNTT 723
           LIWKTQNPWTGLRGQFYDHL DQTAGF+GCRCAAEP+HVQLNL+T  IE      VVNTT
Sbjct: 661 LIWKTQNPWTGLRGQFYDHLHDQTAGFYGCRCAAEPVHVQLNLATYFIE------VVNTT 720

Query: 724 SDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLKL 783
            +E+S VAIE SVWDL+GTCPY+KV E + + PK+   I E++Y  SK++KPVYF+LLKL
Sbjct: 721 HEELSDVAIEVSVWDLDGTCPYYKVIENVLVSPKKVLPITELKYQGSKNAKPVYFVLLKL 780

Query: 784 YNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKSK 843
           +  SN  I+SRNFYWL   G D+K LE YR    P+++TS+V I GS Y++++ VQN SK
Sbjct: 781 FRPSNTTILSRNFYWLRLPGTDFKLLEPYRAIEAPLKLTSEVNIVGSAYKIQMRVQNLSK 840

Query: 844 NAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIV-TGSNNPRLV 903
           N  S S+                 N L N E++D  +  G+  RIC     +G+++ R+V
Sbjct: 841 NLNSESV-----------------NFLANDEKSDLSKKEGYISRICSGFKNSGTDSLRVV 900

Query: 904 ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 963
           ET G   GVAFFLHFSVH+ K +  E ED RILPVHYSDNYFSLVPGE   I ISFE P 
Sbjct: 901 ETKGTGSGVAFFLHFSVHAVKKDENEIEDLRILPVHYSDNYFSLVPGETTNISISFEVPH 946

Query: 964 GVAPKITLHGWNLS 975
           GV P+++L GWN S
Sbjct: 961 GVTPRVSLRGWNCS 946

BLAST of Cp4.1LG04g00580 vs. ExPASy Swiss-Prot
Match: Q75W54 (Mannosylglycoprotein endo-beta-mannosidase OS=Arabidopsis thaliana OX=3702 GN=EBM PE=1 SV=3)

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 670/984 (68.09%), Postives = 790/984 (80.28%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGK + L+ GW+AARSTEV+++G QLTTT+PP+I   S WMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKTV-LDFGWIAARSTEVDVNGVQLTTTNPPAISSESRWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
            +PDPFYGL+NE I DIADSGR+YYTFWFFT FQC+   +Q++ LNFRAINYSA+V++NG
Sbjct: 61  AIPDPFYGLENEAITDIADSGRDYYTFWFFTKFQCQRLLNQYVHLNFRAINYSAQVFVNG 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           H+  LPKGMFRRH+LDV+D++ P+ +NLLA++VHPPDHPG IP +GGQGGDHEIGKDVAA
Sbjct: 121 HKTELPKGMFRRHTLDVTDILHPE-SNLLALIVHPPDHPGTIPPEGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYV+GWDW+ PIRDRNTGIWDEVSIS TGPV+IIDPHLVSTFFDDYKR YLH T E++NK
Sbjct: 181 QYVQGWDWICPIRDRNTGIWDEVSISVTGPVRIIDPHLVSTFFDDYKRAYLHVTAELENK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           S+W  +CSVN+Q+T ELE  +CLVEH+Q++ V +PA   IQ+TF  LYFYKP LWWPNGM
Sbjct: 241 STWNTECSVNIQITAELENGVCLVEHLQTENVLIPAQGRIQHTFKPLYFYKPELWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQ+LY+++I+V V+ FGESDSW   FGFRKIES ID+ TGGRLFK+NG+PIFIRGGNWI
Sbjct: 301 GKQNLYDILITVVVNEFGESDSWMQPFGFRKIESVIDSVTGGRLFKINGEPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLS++RY TDIKFHADMN NMIRCWGGGLAERPEFYH+CDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSKERYRTDIKFHADMNMNMIRCWGGGLAERPEFYHFCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDH+LFLLCARDTVKLLRNHPSLALWVGGNEQVPP DIN ALK+D
Sbjct: 421 DVDGRGVPVSNPNGPLDHELFLLCARDTVKLLRNHPSLALWVGGNEQVPPKDINEALKQD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           L LH +F+T   ++K          DPS YLDGTR+Y+QGSMWDGFA+GKG+FTDGPYEI
Sbjct: 481 LRLHSYFETQLLSDK--------DSDPSVYLDGTRVYIQGSMWDGFADGKGNFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPE+FFKD +YKYGFNPEVGSVGMPVA TIRATMP EGW IPL KK   G+++EVPN +
Sbjct: 541 QYPEDFFKDTYYKYGFNPEVGSVGMPVAETIRATMPPEGWTIPLFKKGLDGFIKEVPNRM 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYS P KV  QI +YG+P++LDDFCLKAQL NYIQYRAL EGW+ +MW KYTG
Sbjct: 601 WDYHKYIPYSNPGKVHDQILMYGTPENLDDFCLKAQLVNYIQYRALFEGWSSQMWTKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
            LIWK QNPWTGLRGQFYDHLLDQTA F+GCR AAEP+HVQLNL++  +E      VVNT
Sbjct: 661 VLIWKNQNPWTGLRGQFYDHLLDQTASFYGCRSAAEPVHVQLNLASYFVE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TS E+S VAIEASVWDL+G CPY+KVF+ +S PPK+   I E +YPK+ + K VYFLLLK
Sbjct: 721 TSKELSDVAIEASVWDLDGNCPYYKVFKIVSAPPKKVVKISEFKYPKAANPKHVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LY VS+  +ISRNFYWLH  G +Y  LE YRK  IP+++T   ++ GS YE+ VNV N S
Sbjct: 781 LYTVSDKAVISRNFYWLHLPGKNYTLLEPYRKKQIPLKITCNAVMVGSRYELEVNVHNTS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPR-- 900
           +                       +N  +N  Q D+KR  G   ++    V  +++ R  
Sbjct: 841 R-----------------------ANLAKNVVQEDEKRDLGLLQKLFSRCVVSADSNRGL 900

Query: 901 -LVETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFE 960
            +VE  G+  GVAFFL FSVH+A+    E +DTRILPVHYSDNYFSLVPGE+M  KISF 
Sbjct: 901 KVVEMKGSDSGVAFFLRFSVHNAET---EKQDTRILPVHYSDNYFSLVPGESMSFKISFA 942

Query: 961 APPGV--APKITLHGWNLSHRLTV 980
           AP G+  +P++ L GWN   R +V
Sbjct: 961 APTGMKKSPRVMLQGWNYPDRFSV 942

BLAST of Cp4.1LG04g00580 vs. ExPASy Swiss-Prot
Match: Q82NR8 (Exo-beta-D-glucosaminidase OS=Streptomyces avermitilis (strain ATCC 31267 / DSM 46492 / JCM 5070 / NBRC 14893 / NCIMB 12804 / NRRL 8165 / MA-4680) OX=227882 GN=csxA PE=1 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 2.0e-58
Identity = 240/971 (24.72%), Postives = 386/971 (39.75%), Query Frame = 0

Query: 39  PSSPWMEAAVPGTVLGTLVKNKVVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLS 98
           P+S W  A    TVL  L+      DPFY    + I        ++   W++ +      
Sbjct: 78  PASGWYPAGARSTVLAALLAGGKYADPFYSTNQQKI-----PKADFQVPWWYRSDFTVAD 137

Query: 99  ESQHLDLNFRAINYSAEVYINGHQ---KVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHP 158
            S    L+F  +  +A+V++NG Q        G + RH LDV+ ++R +G N +A  +  
Sbjct: 138 TSARTYLDFSGVISAADVFVNGRQIARSADVAGAYTRHELDVTSLVR-EGANTVAFRIQ- 197

Query: 159 PDHPGRIPAQGGQGGDHEIGKDVAAQYVEGW-DWMAPIRDRNTGIWDEVSISRTGPVKII 218
           P++P +                       GW DW+ P  D+N GI  +V + R GPV + 
Sbjct: 198 PNNPNK-------------------NLTMGWIDWLEPPPDQNMGIVRDVLVRRGGPVALR 257

Query: 219 DPHLVSTFFDDYKRVYLHATLEMQNKSSWVADCSVNVQVTTELEGNICLVEHIQSQKVSV 278
           D H+++        V   AT ++  K+   A    +  +T  + G++      +S  ++ 
Sbjct: 258 DAHVITRL-----DVPSLATADLTVKAR--ARNDSDAAITATVSGSVGATSFRRSVALAA 317

Query: 279 PAGSTIQYT---FPQLYFYKPNLWWPNGMGKQSLYNVVISVDVDGFGESDSWNHHFGFRK 338
               T+ +T    P L+   P +WWP GMG Q LY + +S  V     SD+ +  FG R 
Sbjct: 318 HETKTVTFTPADTPGLHLTSPRVWWPAGMGGQPLYALDLSASVSE-TVSDTVHESFGIRD 377

Query: 339 IESDIDTATGGRLFKVNGQPIFIRGGNWILSDGLLRLSEKRYHTDIKFHADMNFNMIRCW 398
           +++ ++ + G R + VNG+ + I+GG W   D  LR         +++  D+  N IR  
Sbjct: 378 VKAPLN-SDGARQYSVNGRRLLIKGGGW-SPDEFLRWDSTYVEDRLRYALDLGLNTIRL- 437

Query: 399 GGGLAERPEFYHYCDIYGLLV---WQ--EFWITGDVDGRGVPVSNPNGPLDHDLFLLCAR 458
             G  E  EF+   D YG+L    W+    W  G+V+G G    +     D+ +      
Sbjct: 438 -EGHIEPDEFFDLADRYGILTLPGWECCNKW-EGNVNGSG--SGDEWTAADYPVAKASMA 497

Query: 459 DTVKLLRNHPSLALWVGGNEQVPPPDINTALKKDLELHPHFQTSSENEKWMVDSSSESED 518
                LR+HPS+  ++ G++  P          D ++   +  + +   W     + + D
Sbjct: 498 AEAARLRDHPSVVSFLIGSDFAP----------DAKIEKTYLDALKAADWPTPVVAAASD 557

Query: 519 PSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEIQYPENFF-KDDFYKYGFNPEVGS-VG 578
            S  + G+                G    GPY+   P  ++ K +    GFN E  +   
Sbjct: 558 KSSPVSGS---------------SGMKMTGPYDWIPPNYWYAKREGGATGFNSETSAGPD 617

Query: 579 MPVAATIRATM-PSE---GWEIPLVKKLPAGYVEEVPNPIWDYHKYIPYSKPHKVESQIE 638
           +P   T+R  M P+E    W+ P  K+               YH+      P  V   ++
Sbjct: 618 IPTLDTLRRMMTPAELDTLWKNPGAKQ---------------YHR-----SPSSVFGTLK 677

Query: 639 L--------YGSPKDLDDFCLKAQLANYIQYRALIEGWN---CRMWKKYTGFLIWKTQNP 698
           +        YG+P  L D+  KAQLA Y   RA  E +        K  TG + W   + 
Sbjct: 678 IYDAALAGRYGAPTGLTDYVRKAQLAQYENVRAQFEAYGRGATDASKPATGVIYWMFNSG 737

Query: 699 WTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNTTSDEISGVA 758
           WT L  Q  D  LDQ   +FG + A EP+HVQ +          S +VVN     +SG+ 
Sbjct: 738 WTSLHWQLLDRYLDQGGAYFGAKKANEPLHVQYSYD------DRSVVVVNNRPAAVSGLT 797

Query: 759 IEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLKLYNVSNYGI 818
              ++++ +GT  Y K    LS+      S   +  P S       +L   +   S    
Sbjct: 798 ARVTLFNTDGTQKYDKSATGLSVAGDGAHS-TALTLPSSVSGLSTTYLARLVLTDSAGKE 857

Query: 819 ISRNFYWLHQSGG--DYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKSKNAESSS 878
           +SRN YWL       D+   + Y         TS   +KG     RV V   S  A +++
Sbjct: 858 VSRNVYWLSTRPDTLDWAHTDWYY-----TPTTSYADLKGLGSMARVPV---SATASTTA 899

Query: 879 LTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLVETNGNAV 938
            T            D  S +      T   R+   F                        
Sbjct: 918 GT------------DGASTTTVTVRNTGSGRTPSLF------------------------ 899

Query: 939 GVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP--GVAPK 977
                    VH   ++GK      +LPV +SDN  SL PGE+  + +++      G AP+
Sbjct: 978 -------TDVHLVDSKGKP-----VLPVQWSDNEVSLWPGESATLTVTYRTADLHGSAPR 899

BLAST of Cp4.1LG04g00580 vs. ExPASy Swiss-Prot
Match: Q56F26 (Exo-beta-D-glucosaminidase OS=Amycolatopsis orientalis OX=31958 GN=csxA PE=1 SV=2)

HSP 1 Score: 204.5 bits (519), Expect = 5.4e-51
Identity = 200/784 (25.51%), Postives = 329/784 (41.96%), Query Frame = 0

Query: 39  PSSPWMEAAVPGTVLGTLVKNKVVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLS 98
           P+S W   +   TV   L++N    DPFY    + +        ++   W++ T      
Sbjct: 77  PTSGWYPVSSRSTVYAGLLQNGKYADPFYSTNMQNV-----PAAQFSVPWWYRTDLNVDD 136

Query: 99  ESQHLDLNFRAINYSAEVYINGHQKVLPK----GMFRRHSLDVSDVMRPDGTNLLAVLVH 158
            S    L+F  +   A+V++NG  KV  K    G + RH LD++  +   G N +A  V+
Sbjct: 137 TSSRTYLDFSGVLSKADVWVNG-TKVATKDQVNGAYTRHDLDITAQVH-TGVNSVAFKVY 196

Query: 159 PPDHPGRIPAQGGQGGDHEIGKDVAAQYVEGW-DWMAPIRDRNTGIWDEVSISRTGPVKI 218
           P D P R               D++     GW DW     D+N GI  +V + R+G V +
Sbjct: 197 PND-PNR---------------DLSM----GWIDWAQTPPDQNMGIVRDVLVRRSGAVAL 256

Query: 219 IDPHLVSTFFDDYKRVYLHATLEMQNKSSWVADCSVNVQVTTELEGNICLVEHIQSQKVS 278
              H++           L    +++N S+     +V   V   + G         SQ VS
Sbjct: 257 RSAHVIQKLNSALDHADLTVKADVRNDSA----NAVQTTVAGTVAGKPI------SQTVS 316

Query: 279 VPAGSTIQYTFPQLYFYKPNLWWPNGMGKQSLYNVVISVDVDGFGESDSWNHHFGFRKIE 338
           + A      TFP +   +PN+WWP GMG Q  Y++ ++  V G   SD+    FG R ++
Sbjct: 317 LAAKERKTVTFPLVGLDRPNVWWPAGMGGQHRYDLDLTASVGG-TPSDAAKSKFGVRDVK 376

Query: 339 SDIDTATGGRLFKVNGQPIFIRGGNWILSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGG 398
           + ++ ++GGR + VNG+P+ IRGG +   D  LR +E      +K+  ++  N +R    
Sbjct: 377 ATLN-SSGGRQYSVNGKPLLIRGGGY-TPDLFLRWNETAAADKLKYVLNLGLNTVRL--E 436

Query: 399 GLAERPEFYHYCDIYGLLVWQEFWITGDVDGR--GVPVSNPNGPLDHDLFLLCARDTVKL 458
           G  E  EF+   D  G+L    +      +G+  G     P    D+ +         + 
Sbjct: 437 GHIEPDEFFDIADDLGVLTMPGWECCDKWEGQVNGEEKGEPWVESDYPIAKASMFSEAER 496

Query: 459 LRNHPSLALWVGGNEQVPPPDIN----TALKKDLELHPHFQTSSENEKWMVDSSSESEDP 518
           LR+HPS+  +  G++  P   I      A+K    L P           ++ ++S    P
Sbjct: 497 LRDHPSVISFHIGSDFAPDRRIEQGYLDAMKAADFLLP-----------VIPAASARPSP 556

Query: 519 SQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEIQYPENFF----KDDFYKYGFNPEVGS- 578
                G ++                  +GPY+   P  ++    KD    + FN E  + 
Sbjct: 557 ITGASGMKM------------------NGPYDYVPPVYWYDKSQKDRGGAWSFNSETSAG 616

Query: 579 VGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPIWDYHKYIPYSKPHKV---ESQI 638
           V +P   T++  M +   E+  + K P+            YH+    +  +     ++  
Sbjct: 617 VDIPTMDTLKRMMSAS--ELDTMWKNPSAK---------QYHRSSSDTFGNLKLFGDALT 676

Query: 639 ELYGSPKDLDDFCLKAQLANYIQYRALIEGWN---CRMWKKYTGFLIWKTQNPWTGLRGQ 698
           + YG+  +L+DF  KAQL+ Y   RA  E  +          TG + W   +PWT L  Q
Sbjct: 677 KRYGASANLNDFVRKAQLSQYENVRAEFESHSRNYTDSTNPSTGLIYWMLNSPWTSLHWQ 736

Query: 699 FYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNTTSDEISGVAIEASVWD 758
            +D  +DQ   ++G + A EP+H+Q +        + S +V+N TS+ +SG+     +++
Sbjct: 737 LFDAYMDQNGAYYGAKKANEPLHIQYS------HDNRSVVVINQTSNAVSGLTATTKLYN 767

Query: 759 LEGTCPYFKVFEKLS---LPPKQTSSIVEMEYPKSKDSKPVYFLLLKLYNVSNYGIISRN 798
           L+GT  Y      LS   L  K T+  V    P        Y     L + S    +SRN
Sbjct: 797 LDGTEKYSNTKTGLSVGALGAKATAVTV----PAVSGLSTTYLAKWVLTDSSGKE-VSRN 767

BLAST of Cp4.1LG04g00580 vs. ExPASy Swiss-Prot
Match: Q95327 (Beta-mannosidase OS=Capra hircus OX=9925 GN=MANBA PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 1.6e-34
Identity = 141/593 (23.78%), Postives = 243/593 (40.98%), Query Frame = 0

Query: 38  GPSSPWMEAAVPGTVLGTLVKNKVVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKL 97
           G  S  + AAVPG V   L   +++ DP+Y   N   +D      + +T+         +
Sbjct: 34  GNGSLQLPAAVPGCVHSALFNKRIIKDPYYRFNN---LDYRWIALDNWTYIKKFKLHSDM 93

Query: 98  SESQHLDLNFRAINYSAEVYINGHQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVH--- 157
           SE   ++L F  I+  A V +N         MFRR+S D++ +++    N++ V      
Sbjct: 94  SEWNKVNLVFEGIDTVAVVLLNSVPIGKTDNMFRRYSFDITHMVK--AVNIIEVRFQSPV 153

Query: 158 ---------------PPDHPGRIPAQGGQGGDHEIGKDVAAQYVEGWDWMAPIRDRNTGI 217
                          PP+ P   P Q G+   + I K    Q   GWDW      +  GI
Sbjct: 154 IYANQRSERHTAYWVPPNCPP--PVQDGECHVNFIRK---MQCSFGWDWGPSFPTQ--GI 213

Query: 218 WDEVSISRTGPVKIIDPHLVSTFFDDYKRVY---LHATLEMQNKSSWVADCSVNV-QVTT 277
           W +V I     +  ++  + +  +D+Y   +   + ++ ++ +      +  V + ++  
Sbjct: 214 WKDVRIEAYN-ICHLNYFMFTPIYDNYMETWNLKIESSFDVVSSKLVSGEAIVAIPELNI 273

Query: 278 ELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGMGKQSLYNVVISVDVD 337
           +   NI L    ++ K+ V     +              WWP+G G Q+ Y++ ++ ++D
Sbjct: 274 QQRNNIELRHGERTVKLFVKIDKAVIV----------ETWWPHGHGNQTGYDMTVTFELD 333

Query: 338 G--FGESDSWNHHFGFRKIESDIDTATGGRL-FKVNGQPIFIRGGNWILSDGLL-RLSEK 397
           G    E  +  +      +E  I  + G    FK+NG PIF++G NWI +D    R++  
Sbjct: 334 GGLRFEKSAKVYFRTVELVEEPIQNSPGLTFYFKINGLPIFLKGSNWIPADSFQDRVTSD 393

Query: 398 RYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITGDVDGRGVPVSN 457
                ++   D N N +R WGGG+ E+ EFY  CD  G+++WQ+F     +         
Sbjct: 394 MLRLLLQSVVDANMNALRVWGGGIYEQDEFYELCDELGIMIWQDFMFACAL--------- 453

Query: 458 PNGPLDHDLFLLCARD----TVKLLRNHPSLALWVGGNE----------QVPPPDINTAL 517
              P D D F+   R+     V+ L++HPS+  W G NE             P  ++T +
Sbjct: 454 --YPTDED-FMDSVREEVTHQVRRLKSHPSIITWSGNNENEAALMMGWYDTKPGYLHTYI 513

Query: 518 KKDLELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGF-ANGKGDFTDG 577
           K  + L+       +N + +V             D TR ++  S  +G     +G  +  
Sbjct: 514 KDYVTLY------VKNIRTIVLEG----------DQTRPFIISSPTNGAKTTAEGWLSPN 573

Query: 578 PYEIQYPENFFKD---------DFYKYGFNPEVGSVGMPVAATIRATMPSEGW 581
           PY++ Y +  F D          F K  F  E G    P  +T+      E W
Sbjct: 574 PYDLNYGDVHFYDYMSDCWNWRTFPKARFVSEYGYQSWPSFSTLEKVSSEEDW 575

BLAST of Cp4.1LG04g00580 vs. NCBI nr
Match: XP_023530221.1 (mannosylglycoprotein endo-beta-mannosidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2030 bits (5260), Expect = 0.0
Identity = 974/980 (99.39%), Postives = 974/980 (99.39%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA
Sbjct: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD
Sbjct: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI
Sbjct: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK
Sbjct: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS
Sbjct: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLV 900
           KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLV
Sbjct: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLV 900

Query: 901 ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960
           ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP
Sbjct: 901 ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960

Query: 961 GVAPKITLHGWNLSHRLTVC 980
           GVAPKITLHGWNLSHRLTVC
Sbjct: 961 GVAPKITLHGWNLSHRLTVC 974

BLAST of Cp4.1LG04g00580 vs. NCBI nr
Match: XP_022969264.1 (mannosylglycoprotein endo-beta-mannosidase isoform X1 [Cucurbita maxima])

HSP 1 Score: 2001 bits (5184), Expect = 0.0
Identity = 960/982 (97.76%), Postives = 966/982 (98.37%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA
Sbjct: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNVVIS+DVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVVISIDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCD YGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDTYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN ALKKD
Sbjct: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALKKD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI
Sbjct: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGS+GMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSIGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKPHKV+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPHKVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
            SD+ISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK
Sbjct: 721 MSDDISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LYNVSNYGIISRNFYWLHQSGGDYKQLE YRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS
Sbjct: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLEPYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSL--ENKEQTDKKRSTGFFHRICRHIVTGSNNPR 900
           KNAESSSLTY NNFINRQGEGD DSNSL  ENKEQTDKKRSTGFFHRICR IVTGSN+PR
Sbjct: 841 KNAESSSLTYKNNFINRQGEGDPDSNSLPLENKEQTDKKRSTGFFHRICRRIVTGSNSPR 900

Query: 901 LVETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA 960
           LVETNGNAVGVAFFLHFSVHS+KAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA
Sbjct: 901 LVETNGNAVGVAFFLHFSVHSSKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA 960

Query: 961 PPGVAPKITLHGWNLSHRLTVC 980
           PPGVAPKITLHGWNLSH LTVC
Sbjct: 961 PPGVAPKITLHGWNLSHGLTVC 976

BLAST of Cp4.1LG04g00580 vs. NCBI nr
Match: XP_022926231.1 (mannosylglycoprotein endo-beta-mannosidase isoform X1 [Cucurbita moschata] >XP_022926235.1 mannosylglycoprotein endo-beta-mannosidase isoform X1 [Cucurbita moschata])

HSP 1 Score: 1999 bits (5180), Expect = 0.0
Identity = 957/980 (97.65%), Postives = 964/980 (98.37%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKK KLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKKSKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA
Sbjct: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNVVIS+DV+GFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVVISIDVEGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN ALKKD
Sbjct: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALKKD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           LELHPHFQTSSENEKWMVDSSS SEDPSQYLDGTRIYVQGSMWDGFANGKG FTDGPYEI
Sbjct: 481 LELHPHFQTSSENEKWMVDSSSVSEDPSQYLDGTRIYVQGSMWDGFANGKGGFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPV ATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVVATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKPHKV+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPHKVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TSDE+SG+AIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK
Sbjct: 721 TSDELSGIAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LYNVSNYGIISRNFYWLHQSGGDYKQLE YRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS
Sbjct: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLEPYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLV 900
           KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICR IVTGSN+PRLV
Sbjct: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRRIVTGSNSPRLV 900

Query: 901 ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960
           ETNGNAVGVAFFLHFSVHS+KAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP
Sbjct: 901 ETNGNAVGVAFFLHFSVHSSKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960

Query: 961 GVAPKITLHGWNLSHRLTVC 980
           GV PKITLHGWNLS  LTVC
Sbjct: 961 GVTPKITLHGWNLSQGLTVC 974

BLAST of Cp4.1LG04g00580 vs. NCBI nr
Match: KAG6588476.1 (Mannosylglycoprotein endo-beta-mannosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1998 bits (5177), Expect = 0.0
Identity = 955/980 (97.45%), Postives = 965/980 (98.47%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKK KLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKKSKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINY+AEVYING
Sbjct: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYTAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA
Sbjct: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNVVIS+DV+GFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVVISIDVEGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN ALKKD
Sbjct: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALKKD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           LELHPHFQTSSENEKWMVDSSS SEDPSQYLDGTRIYVQGSMWDGFANGKG FTDGPYEI
Sbjct: 481 LELHPHFQTSSENEKWMVDSSSVSEDPSQYLDGTRIYVQGSMWDGFANGKGGFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGW+IPLVKKLPAGYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWDIPLVKKLPAGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKPHKV+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPHKVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TSDE+SG+AIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK
Sbjct: 721 TSDELSGIAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LYN+SNYGIISRNFYWLHQSGGDYKQLE YRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS
Sbjct: 781 LYNISNYGIISRNFYWLHQSGGDYKQLEPYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLV 900
           KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICR IVTGSN+PRLV
Sbjct: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRRIVTGSNSPRLV 900

Query: 901 ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960
           ETNGNAVGVAFFLHFSVHS+KAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP
Sbjct: 901 ETNGNAVGVAFFLHFSVHSSKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960

Query: 961 GVAPKITLHGWNLSHRLTVC 980
           GV PKITLHGWNLS  LTVC
Sbjct: 961 GVTPKITLHGWNLSQGLTVC 974

BLAST of Cp4.1LG04g00580 vs. NCBI nr
Match: KAG7022305.1 (Mannosylglycoprotein endo-beta-mannosidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1998 bits (5177), Expect = 0.0
Identity = 956/980 (97.55%), Postives = 964/980 (98.37%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKK KLNSGWLAARSTEVELSG QLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKKSKLNSGWLAARSTEVELSGVQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA
Sbjct: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNVVIS+DV+GFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVVISIDVEGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN ALKKD
Sbjct: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALKKD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           LELHPHFQTSSENEKWMVDSSS SEDPSQYLDGTRIYVQGSMWDGFANGKG FTDGPYEI
Sbjct: 481 LELHPHFQTSSENEKWMVDSSSVSEDPSQYLDGTRIYVQGSMWDGFANGKGGFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGW+IPLVKKLPAGYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWDIPLVKKLPAGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKPHKV+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPHKVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TSDE+SG+AIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK
Sbjct: 721 TSDELSGIAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LYNVSNYGIISRNFYWLHQSGGDYKQLE YRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS
Sbjct: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLEPYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLV 900
           KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICR IVTGSN+PRLV
Sbjct: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRRIVTGSNSPRLV 900

Query: 901 ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960
           ETNGNAVGVAFFLHFSVHS+KAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP
Sbjct: 901 ETNGNAVGVAFFLHFSVHSSKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960

Query: 961 GVAPKITLHGWNLSHRLTVC 980
           GV PKITLHGWNLS  LTVC
Sbjct: 961 GVTPKITLHGWNLSQGLTVC 974

BLAST of Cp4.1LG04g00580 vs. ExPASy TrEMBL
Match: A0A6J1I232 (mannosylglycoprotein endo-beta-mannosidase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468316 PE=3 SV=1)

HSP 1 Score: 2001 bits (5184), Expect = 0.0
Identity = 960/982 (97.76%), Postives = 966/982 (98.37%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA
Sbjct: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNVVIS+DVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVVISIDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCD YGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDTYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN ALKKD
Sbjct: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALKKD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI
Sbjct: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGS+GMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSIGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKPHKV+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPHKVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
            SD+ISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK
Sbjct: 721 MSDDISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LYNVSNYGIISRNFYWLHQSGGDYKQLE YRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS
Sbjct: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLEPYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSL--ENKEQTDKKRSTGFFHRICRHIVTGSNNPR 900
           KNAESSSLTY NNFINRQGEGD DSNSL  ENKEQTDKKRSTGFFHRICR IVTGSN+PR
Sbjct: 841 KNAESSSLTYKNNFINRQGEGDPDSNSLPLENKEQTDKKRSTGFFHRICRRIVTGSNSPR 900

Query: 901 LVETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA 960
           LVETNGNAVGVAFFLHFSVHS+KAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA
Sbjct: 901 LVETNGNAVGVAFFLHFSVHSSKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA 960

Query: 961 PPGVAPKITLHGWNLSHRLTVC 980
           PPGVAPKITLHGWNLSH LTVC
Sbjct: 961 PPGVAPKITLHGWNLSHGLTVC 976

BLAST of Cp4.1LG04g00580 vs. ExPASy TrEMBL
Match: A0A6J1EKJ3 (mannosylglycoprotein endo-beta-mannosidase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433409 PE=3 SV=1)

HSP 1 Score: 1999 bits (5180), Expect = 0.0
Identity = 957/980 (97.65%), Postives = 964/980 (98.37%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKK KLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKKSKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA
Sbjct: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNVVIS+DV+GFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVVISIDVEGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN ALKKD
Sbjct: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALKKD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           LELHPHFQTSSENEKWMVDSSS SEDPSQYLDGTRIYVQGSMWDGFANGKG FTDGPYEI
Sbjct: 481 LELHPHFQTSSENEKWMVDSSSVSEDPSQYLDGTRIYVQGSMWDGFANGKGGFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPV ATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVVATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKPHKV+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPHKVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TSDE+SG+AIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK
Sbjct: 721 TSDELSGIAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LYNVSNYGIISRNFYWLHQSGGDYKQLE YRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS
Sbjct: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLEPYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPRLV 900
           KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICR IVTGSN+PRLV
Sbjct: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRRIVTGSNSPRLV 900

Query: 901 ETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960
           ETNGNAVGVAFFLHFSVHS+KAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP
Sbjct: 901 ETNGNAVGVAFFLHFSVHSSKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEAPP 960

Query: 961 GVAPKITLHGWNLSHRLTVC 980
           GV PKITLHGWNLS  LTVC
Sbjct: 961 GVTPKITLHGWNLSQGLTVC 974

BLAST of Cp4.1LG04g00580 vs. ExPASy TrEMBL
Match: A0A6J1CY37 (mannosylglycoprotein endo-beta-mannosidase OS=Momordica charantia OX=3673 GN=LOC111015278 PE=3 SV=1)

HSP 1 Score: 1812 bits (4694), Expect = 0.0
Identity = 864/982 (87.98%), Postives = 917/982 (93.38%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGKKLKLNSGWLAARSTE+EL+G QLTTTHPPSIGPSSPWMEAA+PGTVLGTLVKNK
Sbjct: 1   MAEIGKKLKLNSGWLAARSTEIELTGTQLTTTHPPSIGPSSPWMEAALPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           V+PDPFYGL+NETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSA+VYING
Sbjct: 61  VIPDPFYGLENETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSADVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           H+ VLPKGMFRRHSLDVS+V+ PDGTNLLAVLVHPPDHPGRIP +GGQGGDHEIGKDVAA
Sbjct: 121 HKSVLPKGMFRRHSLDVSEVLHPDGTNLLAVLVHPPDHPGRIPIEGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWMAPIRDRNTGIWDEVSISRTGPV+IIDPHLVSTF+D+Y+RVYLHATLE+QN+
Sbjct: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVRIIDPHLVSTFYDNYERVYLHATLELQNR 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSW ADCSV +QVTTELEGNICLVEH+Q++KVSVPA ST+QYT PQL+FYKPNLWWPN M
Sbjct: 241 SSWDADCSVKIQVTTELEGNICLVEHLQARKVSVPARSTVQYTIPQLFFYKPNLWWPNRM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQSLYNV ISVDVDGFGESDSW+HHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQSLYNVDISVDVDGFGESDSWSHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYH+CDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHFCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVP+SNP+GPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN ALK D
Sbjct: 421 DVDGRGVPISNPDGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALKDD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           L+LHP+FQ SS++E WM  SS    DPSQYLDGTR+Y+QGSMWDGFA+GKG+FTDGPYEI
Sbjct: 481 LKLHPYFQMSSKSENWMGISS----DPSQYLDGTRVYIQGSMWDGFADGKGNFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPVA TIRA MP EGW+IPLV KLP+GYVEEVPN I
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVATTIRAMMPPEGWQIPLVTKLPSGYVEEVPNHI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKP KV+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG
Sbjct: 601 WDYHKYIPYSKPDKVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLST CIE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTYCIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TS+EISGVAIEASVWDLEGTCPY+KVFEKLSLPPKQTSSIVEMEYPKS++ KPVYFLLLK
Sbjct: 721 TSEEISGVAIEASVWDLEGTCPYYKVFEKLSLPPKQTSSIVEMEYPKSENPKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LY VSNYGIISRNFYWLHQSGGDYK LE YR+ NIPIQVTSQV + GSTYEVR+NVQNKS
Sbjct: 781 LYEVSNYGIISRNFYWLHQSGGDYKLLEPYRERNIPIQVTSQVDVTGSTYEVRMNVQNKS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNS--LENKEQTDKKRST-GFFHRICRHIVTGSNNP 900
           KNAESSSLTY NNFI+R G+GD DSNS  L NKEQTD KRS+ G FHRICR I  G+++ 
Sbjct: 841 KNAESSSLTYKNNFIHRHGKGDFDSNSAILGNKEQTDDKRSSAGLFHRICRRIGMGNSSQ 900

Query: 901 RLVETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFE 960
           R VET+GN VGVAFFLHFSVH +K E KEGEDTRILPVHYSDNYFSLVPGE M IKISFE
Sbjct: 901 RSVETDGNDVGVAFFLHFSVHGSKVESKEGEDTRILPVHYSDNYFSLVPGEVMSIKISFE 960

Query: 961 APPGVAPKITLHGWNLSHRLTV 979
           APPGV PKITLHGWN    LT+
Sbjct: 961 APPGVTPKITLHGWNFPQGLTI 972

BLAST of Cp4.1LG04g00580 vs. ExPASy TrEMBL
Match: A0A5A7UB88 (Mannosylglycoprotein endo-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold761G00100 PE=3 SV=1)

HSP 1 Score: 1801 bits (4665), Expect = 0.0
Identity = 862/982 (87.78%), Postives = 907/982 (92.36%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAE+G K+KLNSGWLAARSTEVEL+G QLTTTHPPSI PSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEMGNKVKLNSGWLAARSTEVELTGTQLTTTHPPSITPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGL NETIIDIAD GREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLANETIIDIADCGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           H+KVLPKGMFRRHSLDVS+V+ PDGTNLLAVLVHPPDHPGRIP +GGQGGDHEIGKDVAA
Sbjct: 121 HKKVLPKGMFRRHSLDVSEVLHPDGTNLLAVLVHPPDHPGRIPDKGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWM PIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHAT+E+QN+
Sbjct: 181 QYVEGWDWMTPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATIEIQNR 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSV +QVTTELEGNICLVEH+Q+QKVSVPAGSTIQYT+PQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVKIQVTTELEGNICLVEHLQAQKVSVPAGSTIQYTYPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQ LYNVVIS+DVDGFGESDSW+H FGFRKIES ID  TGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQYLYNVVISIDVDGFGESDSWSHDFGFRKIESHIDPTTGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRG P SNP+GPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDIN AL+ D
Sbjct: 421 DVDGRGDPKSNPDGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINAALQDD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           L+LHPHFQ SS+N +WM  SS   EDPS+YLDGTRIYVQGSMWDGFA+GKG+FTDGPYEI
Sbjct: 481 LKLHPHFQVSSKNNQWMSVSSLTPEDPSEYLDGTRIYVQGSMWDGFADGKGNFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMP EGW+IPLV KLP+GYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPPEGWQIPLVNKLPSGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKP  V+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWN RMWKKYTG
Sbjct: 601 WDYHKYIPYSKPGHVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNSRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLST  IEA FS+IVVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTYRIEAGFSNIVVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TS+EISGVAIEAS WDLEG CPYFKVFEKLSLPPKQT SI EMEYP  ++SKPVYFLLLK
Sbjct: 721 TSNEISGVAIEASAWDLEGMCPYFKVFEKLSLPPKQTLSIAEMEYPTYENSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LY VSN GIISRNFYWLHQSGGDYK+LE YRK N+PIQVTS+V +KGS+YEVR+NVQN S
Sbjct: 781 LYEVSNDGIISRNFYWLHQSGGDYKKLEPYRKINVPIQVTSKVNVKGSSYEVRMNVQNNS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSL--ENKEQTDKKRSTGFFHRICRHIVTGSNNPR 900
           KNAESSSLTY NNFIN QG+GD DSNSL  ENKEQT++K ST FF +I R     +N  R
Sbjct: 841 KNAESSSLTYKNNFINSQGQGDLDSNSLLLENKEQTNEKCSTSFFSKIWRRRSIENNGSR 900

Query: 901 LVETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA 960
           LVETNGN VGVAFFLHF VH +KAE  E  DTRILPV YSDNYFSLVPGEAM I +SFEA
Sbjct: 901 LVETNGNDVGVAFFLHFEVHDSKAEENEEGDTRILPVRYSDNYFSLVPGEAMSINLSFEA 960

Query: 961 PPGVAPKITLHGWNLSHRLTVC 980
           P GV PKITLHGWNLS  L+VC
Sbjct: 961 PLGVTPKITLHGWNLSQSLSVC 982

BLAST of Cp4.1LG04g00580 vs. ExPASy TrEMBL
Match: A0A1S3BPQ9 (mannosylglycoprotein endo-beta-mannosidase OS=Cucumis melo OX=3656 GN=LOC103492174 PE=3 SV=1)

HSP 1 Score: 1787 bits (4628), Expect = 0.0
Identity = 859/982 (87.47%), Postives = 903/982 (91.96%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAE+G K+KLNSGWLAARSTEVEL+G QLTTTHPPSI PSSPWMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEMGNKVKLNSGWLAARSTEVELTGTQLTTTHPPSITPSSPWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
           VVPDPFYGL NETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING
Sbjct: 61  VVPDPFYGLANETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           H+KVLPKGMFRRHSLDVS+V+ PDGTNLLAVLVHPPDHPGRIP +GGQGGDHEIGKDVAA
Sbjct: 121 HKKVLPKGMFRRHSLDVSEVLHPDGTNLLAVLVHPPDHPGRIPDKGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYVEGWDWM PIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHAT+E+QN+
Sbjct: 181 QYVEGWDWMTPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATIEIQNR 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           SSWVADCSV +QVTTELEGNICLVEH+Q+QKVSVPAGSTIQYT+PQLYFYKPNLWWPNGM
Sbjct: 241 SSWVADCSVKIQVTTELEGNICLVEHLQAQKVSVPAGSTIQYTYPQLYFYKPNLWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQ LYNVVIS+DVDGFGESDSW+H FGFRKIES ID  TGGRLFKVNGQPIFIRGGNWI
Sbjct: 301 GKQYLYNVVISIDVDGFGESDSWSHDFGFRKIESHIDPTTGGRLFKVNGQPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRG P SNP+GPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPP DIN AL+ D
Sbjct: 421 DVDGRGDPKSNPDGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPLDINAALQDD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           L+LHPHFQ SS+N +WM  SS   EDPS+YLDGTRIYVQGSMWDGFA+GKG+FTDGPYEI
Sbjct: 481 LKLHPHFQVSSKNNQWMSVSSLTPEDPSEYLDGTRIYVQGSMWDGFADGKGNFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMP EGW+IPLV KLP+GYVEEVPNPI
Sbjct: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPPEGWQIPLVNKLPSGYVEEVPNPI 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYSKP  V+SQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWN RMWKKYTG
Sbjct: 601 WDYHKYIPYSKPGHVQSQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNSRMWKKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
           FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLST  IE      VVNT
Sbjct: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTYRIE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TS+EISGVAIEASVWDLEG CPYFKVFEKLSLPPKQT SI EMEYP  ++SKPVYFLLLK
Sbjct: 721 TSNEISGVAIEASVWDLEGMCPYFKVFEKLSLPPKQTLSIAEMEYPTYENSKPVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LY VSN GIISRNFYWLHQSGGDYK+LE YRK N+PIQVTS+V +KGS+YEVR+NVQN S
Sbjct: 781 LYEVSNDGIISRNFYWLHQSGGDYKKLEPYRKINVPIQVTSKVNVKGSSYEVRMNVQNNS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSL--ENKEQTDKKRSTGFFHRICRHIVTGSNNPR 900
           KNAESSSLTY NNFIN QG+GD DSNSL  ENKEQT++K ST FF +I R     +N  R
Sbjct: 841 KNAESSSLTYKNNFINSQGQGDLDSNSLLLENKEQTNEKCSTSFFSKIWRRRSIENNGSR 900

Query: 901 LVETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFEA 960
           LVETNGN VGVAFFLHF VH +KAE  E  DTRILPV YSDNYFSLVPGEAM I +SFEA
Sbjct: 901 LVETNGNDVGVAFFLHFEVHDSKAEENEEGDTRILPVRYSDNYFSLVPGEAMSINLSFEA 960

Query: 961 PPGVAPKITLHGWNLSHRLTVC 980
           P GV PKITLHGWNLS  L+VC
Sbjct: 961 PLGVTPKITLHGWNLSQSLSVC 976

BLAST of Cp4.1LG04g00580 vs. TAIR 10
Match: AT1G09010.1 (glycoside hydrolase family 2 protein )

HSP 1 Score: 1424.8 bits (3687), Expect = 0.0e+00
Identity = 670/984 (68.09%), Postives = 790/984 (80.28%), Query Frame = 0

Query: 1   MAEIGKKLKLNSGWLAARSTEVELSGAQLTTTHPPSIGPSSPWMEAAVPGTVLGTLVKNK 60
           MAEIGK + L+ GW+AARSTEV+++G QLTTT+PP+I   S WMEAAVPGTVLGTLVKNK
Sbjct: 1   MAEIGKTV-LDFGWIAARSTEVDVNGVQLTTTNPPAISSESRWMEAAVPGTVLGTLVKNK 60

Query: 61  VVPDPFYGLQNETIIDIADSGREYYTFWFFTTFQCKLSESQHLDLNFRAINYSAEVYING 120
            +PDPFYGL+NE I DIADSGR+YYTFWFFT FQC+   +Q++ LNFRAINYSA+V++NG
Sbjct: 61  AIPDPFYGLENEAITDIADSGRDYYTFWFFTKFQCQRLLNQYVHLNFRAINYSAQVFVNG 120

Query: 121 HQKVLPKGMFRRHSLDVSDVMRPDGTNLLAVLVHPPDHPGRIPAQGGQGGDHEIGKDVAA 180
           H+  LPKGMFRRH+LDV+D++ P+ +NLLA++VHPPDHPG IP +GGQGGDHEIGKDVAA
Sbjct: 121 HKTELPKGMFRRHTLDVTDILHPE-SNLLALIVHPPDHPGTIPPEGGQGGDHEIGKDVAA 180

Query: 181 QYVEGWDWMAPIRDRNTGIWDEVSISRTGPVKIIDPHLVSTFFDDYKRVYLHATLEMQNK 240
           QYV+GWDW+ PIRDRNTGIWDEVSIS TGPV+IIDPHLVSTFFDDYKR YLH T E++NK
Sbjct: 181 QYVQGWDWICPIRDRNTGIWDEVSISVTGPVRIIDPHLVSTFFDDYKRAYLHVTAELENK 240

Query: 241 SSWVADCSVNVQVTTELEGNICLVEHIQSQKVSVPAGSTIQYTFPQLYFYKPNLWWPNGM 300
           S+W  +CSVN+Q+T ELE  +CLVEH+Q++ V +PA   IQ+TF  LYFYKP LWWPNGM
Sbjct: 241 STWNTECSVNIQITAELENGVCLVEHLQTENVLIPAQGRIQHTFKPLYFYKPELWWPNGM 300

Query: 301 GKQSLYNVVISVDVDGFGESDSWNHHFGFRKIESDIDTATGGRLFKVNGQPIFIRGGNWI 360
           GKQ+LY+++I+V V+ FGESDSW   FGFRKIES ID+ TGGRLFK+NG+PIFIRGGNWI
Sbjct: 301 GKQNLYDILITVVVNEFGESDSWMQPFGFRKIESVIDSVTGGRLFKINGEPIFIRGGNWI 360

Query: 361 LSDGLLRLSEKRYHTDIKFHADMNFNMIRCWGGGLAERPEFYHYCDIYGLLVWQEFWITG 420
           LSDGLLRLS++RY TDIKFHADMN NMIRCWGGGLAERPEFYH+CDIYGLLVWQEFWITG
Sbjct: 361 LSDGLLRLSKERYRTDIKFHADMNMNMIRCWGGGLAERPEFYHFCDIYGLLVWQEFWITG 420

Query: 421 DVDGRGVPVSNPNGPLDHDLFLLCARDTVKLLRNHPSLALWVGGNEQVPPPDINTALKKD 480
           DVDGRGVPVSNPNGPLDH+LFLLCARDTVKLLRNHPSLALWVGGNEQVPP DIN ALK+D
Sbjct: 421 DVDGRGVPVSNPNGPLDHELFLLCARDTVKLLRNHPSLALWVGGNEQVPPKDINEALKQD 480

Query: 481 LELHPHFQTSSENEKWMVDSSSESEDPSQYLDGTRIYVQGSMWDGFANGKGDFTDGPYEI 540
           L LH +F+T   ++K          DPS YLDGTR+Y+QGSMWDGFA+GKG+FTDGPYEI
Sbjct: 481 LRLHSYFETQLLSDK--------DSDPSVYLDGTRVYIQGSMWDGFADGKGNFTDGPYEI 540

Query: 541 QYPENFFKDDFYKYGFNPEVGSVGMPVAATIRATMPSEGWEIPLVKKLPAGYVEEVPNPI 600
           QYPE+FFKD +YKYGFNPEVGSVGMPVA TIRATMP EGW IPL KK   G+++EVPN +
Sbjct: 541 QYPEDFFKDTYYKYGFNPEVGSVGMPVAETIRATMPPEGWTIPLFKKGLDGFIKEVPNRM 600

Query: 601 WDYHKYIPYSKPHKVESQIELYGSPKDLDDFCLKAQLANYIQYRALIEGWNCRMWKKYTG 660
           WDYHKYIPYS P KV  QI +YG+P++LDDFCLKAQL NYIQYRAL EGW+ +MW KYTG
Sbjct: 601 WDYHKYIPYSNPGKVHDQILMYGTPENLDDFCLKAQLVNYIQYRALFEGWSSQMWTKYTG 660

Query: 661 FLIWKTQNPWTGLRGQFYDHLLDQTAGFFGCRCAAEPIHVQLNLSTCCIEASFSSIVVNT 720
            LIWK QNPWTGLRGQFYDHLLDQTA F+GCR AAEP+HVQLNL++  +E      VVNT
Sbjct: 661 VLIWKNQNPWTGLRGQFYDHLLDQTASFYGCRSAAEPVHVQLNLASYFVE------VVNT 720

Query: 721 TSDEISGVAIEASVWDLEGTCPYFKVFEKLSLPPKQTSSIVEMEYPKSKDSKPVYFLLLK 780
           TS E+S VAIEASVWDL+G CPY+KVF+ +S PPK+   I E +YPK+ + K VYFLLLK
Sbjct: 721 TSKELSDVAIEASVWDLDGNCPYYKVFKIVSAPPKKVVKISEFKYPKAANPKHVYFLLLK 780

Query: 781 LYNVSNYGIISRNFYWLHQSGGDYKQLESYRKSNIPIQVTSQVIIKGSTYEVRVNVQNKS 840
           LY VS+  +ISRNFYWLH  G +Y  LE YRK  IP+++T   ++ GS YE+ VNV N S
Sbjct: 781 LYTVSDKAVISRNFYWLHLPGKNYTLLEPYRKKQIPLKITCNAVMVGSRYELEVNVHNTS 840

Query: 841 KNAESSSLTYNNNFINRQGEGDSDSNSLENKEQTDKKRSTGFFHRICRHIVTGSNNPR-- 900
           +                       +N  +N  Q D+KR  G   ++    V  +++ R  
Sbjct: 841 R-----------------------ANLAKNVVQEDEKRDLGLLQKLFSRCVVSADSNRGL 900

Query: 901 -LVETNGNAVGVAFFLHFSVHSAKAEGKEGEDTRILPVHYSDNYFSLVPGEAMPIKISFE 960
            +VE  G+  GVAFFL FSVH+A+    E +DTRILPVHYSDNYFSLVPGE+M  KISF 
Sbjct: 901 KVVEMKGSDSGVAFFLRFSVHNAET---EKQDTRILPVHYSDNYFSLVPGESMSFKISFA 942

Query: 961 APPGV--APKITLHGWNLSHRLTV 980
           AP G+  +P++ L GWN   R +V
Sbjct: 961 APTGMKKSPRVMLQGWNYPDRFSV 942