Cp4.1LG04g00220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG04g00220
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRNA helicase
LocationCp4.1LG04: 1973875 .. 1979099 (+)
RNA-Seq ExpressionCp4.1LG04g00220
SyntenyCp4.1LG04g00220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAAAGTCGGGAGACGTGTAAGAACCCATCCCATATAATCGTAAATATCTAAAATTTCTGCAATAAAAGAGTTAGGGGGTAAGCCCATTCGGTCCATTCGTTCTATCCTTATCGCTCTGTGTATCACACCCTATAAACCCATCTCCTCTTCCCTCCTCTTCTTCTGGTTTCTCAGTCCCTGATTTTTCTTCAAGGGACTTCGAGAAAGCAAGAGATGGCTTCCATTCTTGGAGTTACTGCAATTCATCATGCGCCAAATCTTGATATGTACAGAAGGATGGGCACGCCGGCGACGGCGACGGTTTCATCCACGCCTTCTCTTTCTTTTGCTGATAAGTCTCACTTCAATTCCTTGAAAGCTTTAACTAGTTTAAGTAGTAGTAGTAGCCGTCGCAACTTCTCCGGTTTCAGCTCTTCTGCAATTGCTACTCCCAATTCCATCCTTAGTGAAGAGGCTTTTAGAAGTCTTGATGGCTTCTCTAAAGACTCGTTGGATGATGATGGTCTTATCGATTCTGAAGCTAATTCTTCTCTTGCATTTTCTGACGATGATGAGCTCGCCATTTCCAAACTTGGCTTGCCCCAACGACTCTCTGATGCTCTTGAGAAGCGTGGGATTACACAACTCTTCGCCATTCAGGTACTCTCTTGCACTCTCTGTTGGTTTTCTTTATTGTCTTCCCTTTTGTTGTGGATTTTCTCCGTTTTCTTCCAGGAATTCTCTTCAGTTTGTTGAAGCTATTAGCTTAAAAATTTATTATCTCTTCTCTTAAGTTCAAGTTTCAAAATGTTCCAGGATGTGTGCAGTGCTCACATTATAATTCAATGTGCAGAAATTTTGAGCAGGCTGCATAACCCTTGCTTGTTTATGAATTGACCATGAATAAGTGTAGTCTTCTTTTATGATATAGATTCATTTTATTCTTAAGTTAGTTAGTCTTCTAGGTTATGTTAATTACACAAGCTGAAGTAAATTCTTTTGAGGGAATCATGTTCGAATCTCAAATATGTTTTGTCACAAGATATATTCGTTGATTAATTTCATTAGTCAAGTGATGTCATCGCATTTTTTTTTTTTTTTTTTTTTTTTCATTTGAAAAATACAGAGAGCTGTTCTTGTCCCTGCACTAGAAGGTCGGGACCTCATTGCTCGTGCGAAGACTGGTACTGGGAAGACATTAGCCTTTGGGATACCGATTATTAAAAAAATCACTGAAGATGATGAATTAAAGAGTCCACGGAGGTATGATTTTGTGACTCCCTGGAATCTGGTACATAGAGATTTGCCTCAGATTTTCTAACTTTTCTGTATTTGTTGACAGGCGTTCACGTCTTCCTAGAGTTTTGGTGTTAACACCCACCAGGGAGCTAGCCAAGCAAGTGGAAAAAGAGATAAAAGAATCTGCACCTTATCTGAACACTGTTTGCGTTTATGGAGGCGTTTCATATGTTACCCAGCAAAGCGCTCTTTCTCGTGGAGTTGATGTGGTAGTTGGAACACCTGGTCGAATAATAGACCTAATAAATGGTAACAGCCTCAATTTGGGTGAAGTTGAATATTTAGTGTTAGATGAAGCAGATCAGATGCTTGCTGTTGGGTTTGAGGAGGATGTGGAAGTAATTTTAGAAAAGCTTCCATCTCAGCGACAAAACATGCTTTTTTCTGCTACTATGCCAACGTGGGTTAAGAAACTTGCACGCAAATATTTGGACAATCCATTGACCATTGATTTGGTAATCTTTTACCTTCTTTTTTATCATCTTAAGTAATTACTTAGGTTTCTGATTTTTGACATCTGCAAATTTATAGGCTGCTTTGTTGTAAATAAACCATGAAATTGTCTTGCCGAAAGATGCATGCTTTTTGGATTACGGTGTACCTGAAGAGACGAGCTTGTTTCTTTTCATACATTGCCTTTTCATTCGTTCATTTTTTTAAAACTTTTCAGTGATATATGAGCATGCAATTTTATTGAAAGAAGTTAATTTGTTATGCATCTCTTATGTGACAATGTTGTCATGACAAGCTAGTGTATGTTAAAAGGAAAAAAGCCATTTACGATCTTAATTGAACAGGATCTGTTTTATAGGTTGTTCTACTGTGTCCTTGAGTTCAAAAGAAAAAGCCAATTGTAATATGAATGCCAACTGCAAGTTTTAAGGGGATGCAACAACTCACTTTGGAGATAGAAGTTGCATTTAACTGTCCATATAAAGATCGATTGCTGCTATGTAAAAATAATCACGCTGTTTCAAATTTGTAGGTTGGCGACCAAGATGAAAAACTTGCTGAAGGGATCAAACTTTATGCCATATTAACTACTGCAACTTCAAAGCAAACCATTCTCAGAGATCTTGTAACAGTAAGTTTTCAAGCATACCCCCCTCCCCCCTCCCCGTCTTCTTCATACACTTTATACCAGTTATTGGGACTTACTCTTTTGTACGTCGTAACTGCAACTCTGTTCTTTGATGTCTTAAGTGGTTAATGGTTAATTCCTTAAGTTTGAACTTTTTGTAAAAAATCAATTTCGATTATCGAAAATCTATCCTTCATCGAGGACAAATGGAAGATTAATTCTTTTATCTCCAAATTCTTAGTATTCACCTCCAAGGTTGTTTTTATACGAAACACTTAGCTTGATCATGTATAGGTATAAGCATGTGCGTGTATGTGTGTATATCTATACATATTCATTCCATTTAAAAAGTTATCTCAATTTTCATGTGATGGTAGAATCACACTTTTTATATGACTGTTTTTTCCTGTTGTAAAAATCATTTTTCTTGTGTGACCCCAATAATGTCATGATTTCAGGTTTATGCTAAGGGGGGCAAGACCATTGTTTTTACGCGGACAAAACGTGATGCTGATGAAGTCTCTTTGGCATTAGCTAATACCATAACATCAGAAGCATTGCATGGAGATATATCACAGCATCAACGGGAAAGAACCTTAAATGGCTTCCGGCAAGGGAAGTTTACTGTCCTGGTTGCAACCGACGTAGCTTCACGAGGATTAGACATTCCAAATGTTGATTTGGTAATTTCATTGGATTCTATTCTTATCTTTGTCAATTCCGTTTGCTATTTTCAATGTGAAACTTATTTCCTTAATCCCCACAAAAAAAGAAAAAAAAACAGGAAAGGTACTCAAACATGTTTTGATTTTTGGTCGTTCTCTAGGTCATAAAGCTATCATGTTCATTTTGGGGTAGTAAACATGTTAAAGTTTAATTTTCATTTTCATTTTTCTTTAGCTTGTAAAGTTAGACCGCAATGCTAAAATGTTCAATTTTTTATAGATTTTTTTTGTGATTTGACTCTCTCTTTACCTTCACTTCAGGTTATCCACTATGAACTTCCTAATGATCCTGAGACTTTCGTACATCGCTCTGGCCGTACTGGCCGTGCAGGAAAAGAGGGTACTGCCATTTTAATGTTTACAAATAGTCAAAGGAGAACAGTTAGATCTCTTGAGCGTGATGTGGGGTGCAAATTTGAATTTGCAAACCCGCCTGGCATTGAAAAGGTCTTAGAATCATCAGCCGAGCAAGTGGTTGTTACTCTTCGTGGAGTTCATCCCGAGTCTATTGAGTTTTTCATTCCAACTGCCCAGAAGTTAATTGATGAACAAGGAGCTGGTGCTCTAGCAGCTGCACTTGCACAATTAAGTGGGTTCTCCCGTCCTCCTTCATCACGATCACTTATCAATCACGAACAGGTAACTAAGTGTTCTGTTTGTGAGATTGATCTAATTAGAAAAACTATATTCCAGATGGCTATGTCATTGAAGCTAAAACATATTTATTGCAGGGATGGGTCACATTGCAGCTCACCAGGGATCCATCTTATTCTCGAGGCTTTTTGTCTGCTAGGTCGGTCACTGGATTTCTGTCTGACGTTTATTCCCCAGCTGCAGATGAAGTTGGAAAAATACATCTAATTGCAGATGAAAGGGTGAATATATCATATCTTAGCATGCTTCACCTATTTCATGCCTTTTGTTTTTAAATTTCCTACGCATATAATTTTGTTTCAACAAAACAATTAAATATTTATGTTGGTAATGCCAGATAAATGGAGCAGTATTTGATCTTCCCGAGGAAATAGCAAAAGAATTGCTGAATAAGGATCTGCCGGAAGGAAATACTCTTTTGAAGATCACCAAGGTATGTGTTTTCTTTCCTCCCCCTTCTCTCACAATCTTAATTGTTGCTTAATTAATAATAGTCCTGTATTGCTTCGAAACTAGTTTTCTTATCTCTTTGATGTATGACCATCATAAACATTGGGCTGTCAACGTACTTTTTGTTTGTAGTTACCTCCTCTGCAAGATGATGGCCCCCCAAGTGACAGCTATGGACGATTTTCTGGCCGAGAAAGAAGTAGCCGAAATAGCTCTAGGGATCGCAGAGGTTCTAGAACATCAAGGGGCTGGGGCGGCAGCAGCCGAGACTCCGATGATGATGGTGATCTATTTAGTCGAAATCGCGGTTTTAGATCTAACAGCAGCAGGGGCCGGAAATTTAAAAGCTCAGGAGATGACTGGCTAATTGGTGGAGGCAGCAGACGATCAAGCAGATCATCGTCCATGGATAGGTAAACTTTTTCTTTACTAATTCATTTGGGAAATTACTTTGCTTCTTTTTTCTTTTTTCTTTTTTCTTTTGCCTAAAAATATTATATTATTTGTAATTTTTCATCAAGAACATGTTGACAACCATATCATATGCTGAATTCTGGATGAGAGTGATGTAATTTGAATATACATATACATATTGCAGATTTGGAGGGTCCTGTTTTAACTGCGGAAGGATGGGGCACAGGGCATCAGAATGCCCCAACAATTGAGAGGAGCACGGCCATCAATCAAATATACTTTTGATTCAGCTTTTAGTTTCTTGTGGCGACTTATAAGTAAGTAGGTTTTTGGTGTCAGGTAGGCTATAGGTTGCATTTATCTTAATATTCAGTGGCGACTACAATATGCTCTCCTTGAAAGAGTTGCAGGAATGATGCTCACTCTCATGCTGCGTTACAACATCACCAACAATTCTCAGTCTGTTGTCCTATTAAGTTTTGCGCAGTGATGCATTTAGAAGTTGATTGTTTTGTGTATGAATTGAATTATATGAATCCCCCCCTTTTCGCCCCCATGTAGAATGTATATGTTCCAGAGACAGAATGAAGAAATGAAAACAGCATAGCATTCCCTCGTTAT

mRNA sequence

GAAAAAGTCGGGAGACGTGTAAGAACCCATCCCATATAATCGTAAATATCTAAAATTTCTGCAATAAAAGAGTTAGGGGGTAAGCCCATTCGGTCCATTCGTTCTATCCTTATCGCTCTGTGTATCACACCCTATAAACCCATCTCCTCTTCCCTCCTCTTCTTCTGGTTTCTCAGTCCCTGATTTTTCTTCAAGGGACTTCGAGAAAGCAAGAGATGGCTTCCATTCTTGGAGTTACTGCAATTCATCATGCGCCAAATCTTGATATGTACAGAAGGATGGGCACGCCGGCGACGGCGACGGTTTCATCCACGCCTTCTCTTTCTTTTGCTGATAAGTCTCACTTCAATTCCTTGAAAGCTTTAACTAGTTTAAGTAGTAGTAGTAGCCGTCGCAACTTCTCCGGTTTCAGCTCTTCTGCAATTGCTACTCCCAATTCCATCCTTAGTGAAGAGGCTTTTAGAAGTCTTGATGGCTTCTCTAAAGACTCGTTGGATGATGATGGTCTTATCGATTCTGAAGCTAATTCTTCTCTTGCATTTTCTGACGATGATGAGCTCGCCATTTCCAAACTTGGCTTGCCCCAACGACTCTCTGATGCTCTTGAGAAGCGTGGGATTACACAACTCTTCGCCATTCAGAGAGCTGTTCTTGTCCCTGCACTAGAAGGTCGGGACCTCATTGCTCGTGCGAAGACTGGTACTGGGAAGACATTAGCCTTTGGGATACCGATTATTAAAAAAATCACTGAAGATGATGAATTAAAGAGTCCACGGAGGCGTTCACGTCTTCCTAGAGTTTTGGTGTTAACACCCACCAGGGAGCTAGCCAAGCAAGTGGAAAAAGAGATAAAAGAATCTGCACCTTATCTGAACACTGTTTGCGTTTATGGAGGCGTTTCATATGTTACCCAGCAAAGCGCTCTTTCTCGTGGAGTTGATGTGGTAGTTGGAACACCTGGTCGAATAATAGACCTAATAAATGGTAACAGCCTCAATTTGGGTGAAGTTGAATATTTAGTGTTAGATGAAGCAGATCAGATGCTTGCTGTTGGGTTTGAGGAGGATGTGGAAGTAATTTTAGAAAAGCTTCCATCTCAGCGACAAAACATGCTTTTTTCTGCTACTATGCCAACGTGGGTTAAGAAACTTGCACGCAAATATTTGGACAATCCATTGACCATTGATTTGGTTGGCGACCAAGATGAAAAACTTGCTGAAGGGATCAAACTTTATGCCATATTAACTACTGCAACTTCAAAGCAAACCATTCTCAGAGATCTTGTAACAGTTTATGCTAAGGGGGGCAAGACCATTGTTTTTACGCGGACAAAACGTGATGCTGATGAAGTCTCTTTGGCATTAGCTAATACCATAACATCAGAAGCATTGCATGGAGATATATCACAGCATCAACGGGAAAGAACCTTAAATGGCTTCCGGCAAGGGAAGTTTACTGTCCTGGTTGCAACCGACGTAGCTTCACGAGGATTAGACATTCCAAATGTTGATTTGGTTATCCACTATGAACTTCCTAATGATCCTGAGACTTTCGTACATCGCTCTGGCCGTACTGGCCGTGCAGGAAAAGAGGGTACTGCCATTTTAATGTTTACAAATAGTCAAAGGAGAACAGTTAGATCTCTTGAGCGTGATGTGGGGTGCAAATTTGAATTTGCAAACCCGCCTGGCATTGAAAAGGTCTTAGAATCATCAGCCGAGCAAGTGGTTGTTACTCTTCGTGGAGTTCATCCCGAGTCTATTGAGTTTTTCATTCCAACTGCCCAGAAGTTAATTGATGAACAAGGAGCTGGTGCTCTAGCAGCTGCACTTGCACAATTAAGTGGGTTCTCCCGTCCTCCTTCATCACGATCACTTATCAATCACGAACAGGGATGGGTCACATTGCAGCTCACCAGGGATCCATCTTATTCTCGAGGCTTTTTGTCTGCTAGGTCGGTCACTGGATTTCTGTCTGACGTTTATTCCCCAGCTGCAGATGAAGTTGGAAAAATACATCTAATTGCAGATGAAAGGATAAATGGAGCAGTATTTGATCTTCCCGAGGAAATAGCAAAAGAATTGCTGAATAAGGATCTGCCGGAAGGAAATACTCTTTTGAAGATCACCAAGTTACCTCCTCTGCAAGATGATGGCCCCCCAAGTGACAGCTATGGACGATTTTCTGGCCGAGAAAGAAGTAGCCGAAATAGCTCTAGGGATCGCAGAGGTTCTAGAACATCAAGGGGCTGGGGCGGCAGCAGCCGAGACTCCGATGATGATGGTGATCTATTTAGTCGAAATCGCGGTTTTAGATCTAACAGCAGCAGGGGCCGGAAATTTAAAAGCTCAGGAGATGACTGGCTAATTGGTGGAGGCAGCAGACGATCAAGCAGATCATCGTCCATGGATAGATTTGGAGGGTCCTGTTTTAACTGCGGAAGGATGGGGCACAGGGCATCAGAATGCCCCAACAATTGAGAGGAGCACGGCCATCAATCAAATATACTTTTGATTCAGCTTTTAGTTTCTTGTGGCGACTTATAAAGTTGCAGGAATGATGCTCACTCTCATGCTGCGTTACAACATCACCAACAATTCTCAGTCTGTTGTCCTATTAAGTTTTGCGCAGTGATGCATTTAGAAGTTGATTGTTTTGTGTATGAATTGAATTATATGAATCCCCCCCTTTTCGCCCCCATGTAGAATGTATATGTTCCAGAGACAGAATGAAGAAATGAAAACAGCATAGCATTCCCTCGTTAT

Coding sequence (CDS)

ATGGCTTCCATTCTTGGAGTTACTGCAATTCATCATGCGCCAAATCTTGATATGTACAGAAGGATGGGCACGCCGGCGACGGCGACGGTTTCATCCACGCCTTCTCTTTCTTTTGCTGATAAGTCTCACTTCAATTCCTTGAAAGCTTTAACTAGTTTAAGTAGTAGTAGTAGCCGTCGCAACTTCTCCGGTTTCAGCTCTTCTGCAATTGCTACTCCCAATTCCATCCTTAGTGAAGAGGCTTTTAGAAGTCTTGATGGCTTCTCTAAAGACTCGTTGGATGATGATGGTCTTATCGATTCTGAAGCTAATTCTTCTCTTGCATTTTCTGACGATGATGAGCTCGCCATTTCCAAACTTGGCTTGCCCCAACGACTCTCTGATGCTCTTGAGAAGCGTGGGATTACACAACTCTTCGCCATTCAGAGAGCTGTTCTTGTCCCTGCACTAGAAGGTCGGGACCTCATTGCTCGTGCGAAGACTGGTACTGGGAAGACATTAGCCTTTGGGATACCGATTATTAAAAAAATCACTGAAGATGATGAATTAAAGAGTCCACGGAGGCGTTCACGTCTTCCTAGAGTTTTGGTGTTAACACCCACCAGGGAGCTAGCCAAGCAAGTGGAAAAAGAGATAAAAGAATCTGCACCTTATCTGAACACTGTTTGCGTTTATGGAGGCGTTTCATATGTTACCCAGCAAAGCGCTCTTTCTCGTGGAGTTGATGTGGTAGTTGGAACACCTGGTCGAATAATAGACCTAATAAATGGTAACAGCCTCAATTTGGGTGAAGTTGAATATTTAGTGTTAGATGAAGCAGATCAGATGCTTGCTGTTGGGTTTGAGGAGGATGTGGAAGTAATTTTAGAAAAGCTTCCATCTCAGCGACAAAACATGCTTTTTTCTGCTACTATGCCAACGTGGGTTAAGAAACTTGCACGCAAATATTTGGACAATCCATTGACCATTGATTTGGTTGGCGACCAAGATGAAAAACTTGCTGAAGGGATCAAACTTTATGCCATATTAACTACTGCAACTTCAAAGCAAACCATTCTCAGAGATCTTGTAACAGTTTATGCTAAGGGGGGCAAGACCATTGTTTTTACGCGGACAAAACGTGATGCTGATGAAGTCTCTTTGGCATTAGCTAATACCATAACATCAGAAGCATTGCATGGAGATATATCACAGCATCAACGGGAAAGAACCTTAAATGGCTTCCGGCAAGGGAAGTTTACTGTCCTGGTTGCAACCGACGTAGCTTCACGAGGATTAGACATTCCAAATGTTGATTTGGTTATCCACTATGAACTTCCTAATGATCCTGAGACTTTCGTACATCGCTCTGGCCGTACTGGCCGTGCAGGAAAAGAGGGTACTGCCATTTTAATGTTTACAAATAGTCAAAGGAGAACAGTTAGATCTCTTGAGCGTGATGTGGGGTGCAAATTTGAATTTGCAAACCCGCCTGGCATTGAAAAGGTCTTAGAATCATCAGCCGAGCAAGTGGTTGTTACTCTTCGTGGAGTTCATCCCGAGTCTATTGAGTTTTTCATTCCAACTGCCCAGAAGTTAATTGATGAACAAGGAGCTGGTGCTCTAGCAGCTGCACTTGCACAATTAAGTGGGTTCTCCCGTCCTCCTTCATCACGATCACTTATCAATCACGAACAGGGATGGGTCACATTGCAGCTCACCAGGGATCCATCTTATTCTCGAGGCTTTTTGTCTGCTAGGTCGGTCACTGGATTTCTGTCTGACGTTTATTCCCCAGCTGCAGATGAAGTTGGAAAAATACATCTAATTGCAGATGAAAGGATAAATGGAGCAGTATTTGATCTTCCCGAGGAAATAGCAAAAGAATTGCTGAATAAGGATCTGCCGGAAGGAAATACTCTTTTGAAGATCACCAAGTTACCTCCTCTGCAAGATGATGGCCCCCCAAGTGACAGCTATGGACGATTTTCTGGCCGAGAAAGAAGTAGCCGAAATAGCTCTAGGGATCGCAGAGGTTCTAGAACATCAAGGGGCTGGGGCGGCAGCAGCCGAGACTCCGATGATGATGGTGATCTATTTAGTCGAAATCGCGGTTTTAGATCTAACAGCAGCAGGGGCCGGAAATTTAAAAGCTCAGGAGATGACTGGCTAATTGGTGGAGGCAGCAGACGATCAAGCAGATCATCGTCCATGGATAGATTTGGAGGGTCCTGTTTTAACTGCGGAAGGATGGGGCACAGGGCATCAGAATGCCCCAACAATTGA

Protein sequence

MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN
Homology
BLAST of Cp4.1LG04g00220 vs. ExPASy Swiss-Prot
Match: Q8L7S8 (DEAD-box ATP-dependent RNA helicase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RH3 PE=1 SV=2)

HSP 1 Score: 967.2 bits (2499), Expect = 1.1e-280
Identity = 539/759 (71.01%), Postives = 611/759 (80.50%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MAS +GV +++  P+L++ +    P +   S+  SLS  DK  F  L    SL   + R 
Sbjct: 1   MASTVGVPSLYQVPHLEISK----PNSKKRSNCLSLSL-DKPFFTPL----SLVRRTRRI 60

Query: 61  NFSG-FSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISK 120
           + S     SA+ATPNS+LSEEAF+SL     D  D DG      N+++   D +ELAISK
Sbjct: 61  HSSSLLVPSAVATPNSVLSEEAFKSLGLSDHDEYDLDG-----DNNNVEADDGEELAISK 120

Query: 121 LGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITE 180
           L LPQRL ++LEKRGIT LF IQRAVLVPAL+GRD+IARAKTGTGKTLAFGIPIIK++TE
Sbjct: 121 LSLPQRLEESLEKRGITHLFPIQRAVLVPALQGRDIIARAKTGTGKTLAFGIPIIKRLTE 180

Query: 181 D-DELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240
           +  +  + RR  RLP+ LVL PTRELAKQVEKEIKESAPYL+TVCVYGGVSY  QQSAL+
Sbjct: 181 EAGDYTAFRRSGRLPKFLVLAPTRELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQSALT 240

Query: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300
           RGVDVVVGTPGRIIDLI G SL LGEVEYLVLDEADQMLAVGFEE VE ILE LP++RQ+
Sbjct: 241 RGVDVVVGTPGRIIDLIEGRSLKLGEVEYLVLDEADQMLAVGFEEAVESILENLPTKRQS 300

Query: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360
           MLFSATMPTWVKKLARKYLDNPL IDLVGDQDEKLAEGIKLYAI TT+TSK+TIL DL+T
Sbjct: 301 MLFSATMPTWVKKLARKYLDNPLNIDLVGDQDEKLAEGIKLYAIATTSTSKRTILSDLIT 360

Query: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420
           VYAKGGKTIVFT+TKRDADEVSLAL+N+I +EALHGDISQHQRERTLN FRQGKFTVLVA
Sbjct: 361 VYAKGGKTIVFTQTKRDADEVSLALSNSIATEALHGDISQHQRERTLNAFRQGKFTVLVA 420

Query: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480
           TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEG+AILM T+SQ+RTVRSLE
Sbjct: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGSAILMHTSSQKRTVRSLE 480

Query: 481 RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAA 540
           RDVGC FEF +PP +  +LESSA+QVV TL GVHP+SI+FF  TAQKL +E+G  ALAAA
Sbjct: 481 RDVGCHFEFISPPTVGDLLESSADQVVATLNGVHPDSIKFFSATAQKLYEEKGTDALAAA 540

Query: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600
           LA LSGFS+PPSSRSL++HE+GWVTLQL RDP+ +RGFLSARSVTGFLSD+Y  AADEVG
Sbjct: 541 LAHLSGFSQPPSSRSLLSHEKGWVTLQLIRDPTNARGFLSARSVTGFLSDLYRTAADEVG 600

Query: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660
           KI LIAD+RI GAVFDLPEEIAKELL KD+PEGN+L  ITKLPPLQDDGP SD+YGRFS 
Sbjct: 601 KIFLIADDRIQGAVFDLPEEIAKELLEKDVPEGNSLSMITKLPPLQDDGPSSDNYGRFSS 660

Query: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDS---DDDGDLFSRNRGFRSNSSRGRKFKSSGDD 720
           R+R  R      RGSR  RG     RDS   DDD      + G  S S  G   + S DD
Sbjct: 661 RDRMPRGGG-GSRGSRGGRGGSSRGRDSWGGDDDRGSRRSSGGGSSWSRGGSSSRGSSDD 720

Query: 721 WLIGGGSRRSSRSSSMDR-FGGSCFNCGRMGHRASECPN 754
           WLIGG S  SSR+ S +R FGGSCF CG+ GHRA++CP+
Sbjct: 721 WLIGGRSSSSSRAPSRERSFGGSCFICGKSGHRATDCPD 744

BLAST of Cp4.1LG04g00220 vs. ExPASy Swiss-Prot
Match: Q0DM51 (DEAD-box ATP-dependent RNA helicase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0827700 PE=2 SV=2)

HSP 1 Score: 921.8 bits (2381), Expect = 5.1e-267
Identity = 510/752 (67.82%), Postives = 588/752 (78.19%), Query Frame = 0

Query: 25  PATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRRNFSG--FSSSAIATPNSILSEEAF 84
           P+ +  + + S + A      SL+   +    + RR   G   ++ AIA+PNS+LSE AF
Sbjct: 8   PSLSLSNPSASAAAAGAGAAPSLRLRAAFRCWALRRAGGGRWAAAGAIASPNSVLSEHAF 67

Query: 85  RSLDGFSKDSLDDDGLIDSEANSSLAF----SDDDELAISKLGLPQRLSDALEKRGITQL 144
           + L    ++  +++G   S+     A      D+DELAI++LGLP++L   LEKRGIT L
Sbjct: 68  KRLQLSDEEEEEEEGAYGSDEEGVEAVGGGEGDEDELAIARLGLPEQLVSTLEKRGITHL 127

Query: 145 FAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITEDDELKSPRRRSRLPRVLVL 204
           F IQRAVL+PAL+GRDLIARAKTGTGKTLAFGIP+IK++ E+D+ +S  RR R+PRVLVL
Sbjct: 128 FPIQRAVLIPALDGRDLIARAKTGTGKTLAFGIPMIKQLMEEDDGRSV-RRGRIPRVLVL 187

Query: 205 TPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRGVDVVVGTPGRIIDLINGN 264
            PTRELAKQVEKEIKESAP L+TVCVYGGVSY  QQ+ALSRGVDVVVGTPGRIIDLING 
Sbjct: 188 APTRELAKQVEKEIKESAPKLSTVCVYGGVSYNVQQNALSRGVDVVVGTPGRIIDLINGG 247

Query: 265 SLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNMLFSATMPTWVKKLARKYLD 324
           SL LGEV+YLVLDEADQMLAVGFEEDVE IL++LP++RQ+MLFSATMP WVKKL+R+YL+
Sbjct: 248 SLQLGEVKYLVLDEADQMLAVGFEEDVETILQQLPAERQSMLFSATMPGWVKKLSRRYLN 307

Query: 325 NPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVYAKGGKTIVFTRTKRDADE 384
           NPLTIDLVGDQDEKLAEGIKLYAI  T+TSK+T+L DL+TVYAKGGKTIVFT+TKRDADE
Sbjct: 308 NPLTIDLVGDQDEKLAEGIKLYAIPLTSTSKRTVLSDLITVYAKGGKTIVFTKTKRDADE 367

Query: 385 VSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYE 444
           VSLAL N+I SEALHGDISQHQRERTLNGFRQGKFTVLVATDVA+RGLDIPNVDL+IHYE
Sbjct: 368 VSLALTNSIASEALHGDISQHQRERTLNGFRQGKFTVLVATDVAARGLDIPNVDLIIHYE 427

Query: 445 LPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERDVGCKFEFANPPGIEKVLE 504
           LPNDPETFVHRSGRTGRAGK GTAILMFTNSQRRTVRSLERDVGC+F+F +PP IE VLE
Sbjct: 428 LPNDPETFVHRSGRTGRAGKAGTAILMFTNSQRRTVRSLERDVGCRFDFISPPAIEDVLE 487

Query: 505 SSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALAQLSGFSRPPSSRSLINHE 564
           SSAE V+ TLRGVH ESI++FIP A++L +E G  ALA+ALA LSGFS+PPSSRSLI+HE
Sbjct: 488 SSAEHVIATLRGVHTESIQYFIPAAERLQEELGPNALASALAHLSGFSQPPSSRSLISHE 547

Query: 565 QGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKIHLIADERINGAVFDLPEE 624
           QGWVTLQLTRDP Y RGF S RSVTGFLSDV S AADEVGKI L ADE++ GAVFDLPEE
Sbjct: 548 QGWVTLQLTRDPGYGRGFFSPRSVTGFLSDVSSAAADEVGKIFLTADEKVQGAVFDLPEE 607

Query: 625 IAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRERSSRNSSRDRRGSRTSR- 684
           IA++LL+ +LP GNT+ K+TKLP LQDDGP +DSYGRFS  +R  RN      GSR  R 
Sbjct: 608 IARDLLSMELPPGNTITKVTKLPALQDDGPATDSYGRFSNSDRGFRNRRSRGGGSRGGRG 667

Query: 685 GW----------GG----SSRDSDDDGDLFSRNRGFRSNS--SRGRKFKSSGDDWLIGGG 744
           GW          GG    S  DS  D D     R  RS+S   RG  + S       G  
Sbjct: 668 GWDSDGEDRFRRGGRSFRSDNDSWSDDDFGGGRRSNRSSSFGGRGSSYGSRSSSSFGGRS 727

Query: 745 SRRSSRSSSMDRFGGSCFNCGRMGHRASECPN 754
           S   SR SS   F G+CFNCG  GHRAS+CPN
Sbjct: 728 SSFGSRDSSRS-FSGACFNCGESGHRASDCPN 757

BLAST of Cp4.1LG04g00220 vs. ExPASy Swiss-Prot
Match: A0A1D6LAB7 (DEAD-box ATP-dependent RNA helicase 3B, chloroplastic OS=Zea mays OX=4577 GN=RH3B PE=2 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 2.0e-263
Identity = 499/718 (69.50%), Postives = 566/718 (78.83%), Query Frame = 0

Query: 66  SSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSL--AFSDDDELAISKLGLP 125
           ++ A+A+PNS+LSE AF+ L     D  D +G   S+A+        D++ELAI++LGLP
Sbjct: 38  AAGALASPNSVLSEHAFKRLQ-LGSDDEDGEGPYGSDADEGFEAGEGDNEELAIARLGLP 97

Query: 126 QRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITEDDEL 185
             L   LEKRGIT LF IQRAVL+PALEGRDLIARAKTGTGKTLAFGIP+IK++ E D+ 
Sbjct: 98  DELVATLEKRGITHLFPIQRAVLIPALEGRDLIARAKTGTGKTLAFGIPMIKQLIEQDDG 157

Query: 186 KSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRGVDV 245
           +   RR R PRVLVL PTRELAKQVEKEIKESAP L TVCVYGGVSY  QQ+ALSRGVDV
Sbjct: 158 RI-TRRGRTPRVLVLAPTRELAKQVEKEIKESAPKLGTVCVYGGVSYNVQQNALSRGVDV 217

Query: 246 VVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNMLFSA 305
           VVGTPGRIIDLING SL LGEV+YLVLDEADQMLAVGFEEDVE IL++LP+ RQ+MLFSA
Sbjct: 218 VVGTPGRIIDLINGGSLQLGEVQYLVLDEADQMLAVGFEEDVETILQQLPAGRQSMLFSA 277

Query: 306 TMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVYAKG 365
           TMP+WVKKL+R+YL+NPLTIDLVGDQDEKLAEGIKLYAI  T TSK+T+L DL+TVYAKG
Sbjct: 278 TMPSWVKKLSRRYLNNPLTIDLVGDQDEKLAEGIKLYAIPLTTTSKRTVLSDLITVYAKG 337

Query: 366 GKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATDVAS 425
           GKTIVFTRTK+DADEVSLAL N+I SEALHGDISQHQRERTLNGFRQGKFTVLVATDVA+
Sbjct: 338 GKTIVFTRTKKDADEVSLALTNSIASEALHGDISQHQRERTLNGFRQGKFTVLVATDVAA 397

Query: 426 RGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERDVGC 485
           RGLDIPNVDL+IHYELPNDPETFVHRSGRTGRAGK GTAILMFT+SQ+RTV+SLERDVGC
Sbjct: 398 RGLDIPNVDLIIHYELPNDPETFVHRSGRTGRAGKAGTAILMFTSSQKRTVKSLERDVGC 457

Query: 486 KFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALAQLS 545
            FEF +PP IE+VLESSAE V+ TLRGVHPES ++F+  A+KL +E G  ALA+ALA LS
Sbjct: 458 NFEFISPPSIEEVLESSAEHVIATLRGVHPESTKYFLGAAEKLTEELGPHALASALAHLS 517

Query: 546 GFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKIHLI 605
           GFS+PPSSRSLI+HEQGWVTLQLTR+  + RGF S RSVTGFLSDV S AADEVGKI+L 
Sbjct: 518 GFSQPPSSRSLISHEQGWVTLQLTREQGFGRGFFSPRSVTGFLSDVCSAAADEVGKIYLT 577

Query: 606 ADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRERSS 665
           ADE + GAVFDLPEEIAK+LL  +LP GNTL KI+KLP LQDDGP +DSYGRFS  +R S
Sbjct: 578 ADENVQGAVFDLPEEIAKDLLTMELPPGNTLTKISKLPALQDDGPATDSYGRFS-NDRGS 637

Query: 666 RNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIGGGSR 725
           RN+ R  RG   SRG GG   D +D        RG RS  S    +  S DDW   GG R
Sbjct: 638 RNNRRS-RGGGASRGRGGWDTDGED-----RFRRGGRSLRSDNDSW--SDDDW--SGGGR 697

Query: 726 RSSRSSSMD----------------------------RFGGSCFNCGRMGHRASECPN 754
           +S+RSSS                               F G+CFNCG  GHRA++CPN
Sbjct: 698 KSNRSSSFGSRSSSYSSRGSPSFGGRSSSFGGRESNRSFSGACFNCGESGHRATDCPN 742

BLAST of Cp4.1LG04g00220 vs. ExPASy Swiss-Prot
Match: A0A1D6GDY8 (DEAD-box ATP-dependent RNA helicase 3A, chloroplastic OS=Zea mays OX=4577 GN=RH3A PE=3 SV=1)

HSP 1 Score: 904.4 bits (2336), Expect = 8.4e-262
Identity = 507/760 (66.71%), Postives = 588/760 (77.37%), Query Frame = 0

Query: 28  ATVSSTPSLSFADKSHFNS---LKALTSLSSSSSRRNFSGFSSSAIATPNSILSEEAFRS 87
           A++ + P+++F++ +  +    L+A      +  RR ++   ++A+A+PNS+LSE AF+ 
Sbjct: 2   ASLVTLPAIAFSNPATASGAVRLRAAAFRCWALRRRGWA--VAAAVASPNSVLSEHAFKR 61

Query: 88  LDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISKLGLPQRLSDALEKRGITQLFAIQRA 147
           L     D  D++G   S+A+      D++ELAI++LGLP  L   LEKRGIT LF IQRA
Sbjct: 62  LQ-LGSDDEDEEGPYGSDADEGFQ-GDEEELAIARLGLPDELVATLEKRGITHLFPIQRA 121

Query: 148 VLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITEDDELKSPRRRSRLPRVLVLTPTREL 207
           VL+PAL GRDLIARAKTGTGKTLAFGIP+IK++ E D+ +S  RR R PRVLVL PTREL
Sbjct: 122 VLIPALGGRDLIARAKTGTGKTLAFGIPMIKQLMEQDDGRS-TRRGRTPRVLVLAPTREL 181

Query: 208 AKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRGVDVVVGTPGRIIDLINGNSLNLGE 267
           AKQVEKEIKESAP L TVCVYGGVSY  QQ+ALSRGVDVVVGTPGRIIDLING SL LGE
Sbjct: 182 AKQVEKEIKESAPKLGTVCVYGGVSYNVQQNALSRGVDVVVGTPGRIIDLINGGSLQLGE 241

Query: 268 VEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNMLFSATMPTWVKKLARKYLDNPLTID 327
           V+YLVLDEADQMLAVGFEEDVE IL++LP+ RQ+MLFSATMP+WVKKL+R+YL+NPLTID
Sbjct: 242 VQYLVLDEADQMLAVGFEEDVETILQQLPADRQSMLFSATMPSWVKKLSRRYLNNPLTID 301

Query: 328 LVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVYAKGGKTIVFTRTKRDADEVSLALA 387
           LVGDQDEKLAEGIKL+AI  TATSK+TIL DL+TVYAKGGKTIVFTRTK+DADEVSLAL 
Sbjct: 302 LVGDQDEKLAEGIKLHAIPLTATSKRTILSDLITVYAKGGKTIVFTRTKKDADEVSLALT 361

Query: 388 NTITSEALHGDISQHQRERTLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPNDPE 447
            +I SEALHGDISQHQRERTLNGFRQGKFTVLVATDVA+RGLDIPNVDL+IHYELPNDPE
Sbjct: 362 TSIASEALHGDISQHQRERTLNGFRQGKFTVLVATDVAARGLDIPNVDLIIHYELPNDPE 421

Query: 448 TFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERDVGCKFEFANPPGIEKVLESSAEQV 507
           TFVHRSGRTGRAGK GTAILMFT+SQ+RTV SLERDVGCKFEF +PP IE+VLESSAE V
Sbjct: 422 TFVHRSGRTGRAGKAGTAILMFTSSQKRTVMSLERDVGCKFEFISPPSIEEVLESSAEHV 481

Query: 508 VVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALAQLSGFSRPPSSRSLINHEQGWVTL 567
           + TLRGVHPES ++F+  A+KL +E G  ALA+ALA LSGFS+PPSSRSLI++EQGWVTL
Sbjct: 482 IATLRGVHPESTQYFLGAAEKLTEELGPHALASALAHLSGFSQPPSSRSLISYEQGWVTL 541

Query: 568 QLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKIHLIADERINGAVFDLPEEIAKELL 627
           QLTR+P Y RGF S RSVTGFLSDV S AADEVGKI++ ADE + GAVFDLPEEIAK+LL
Sbjct: 542 QLTREPGYGRGFFSPRSVTGFLSDVCSAAADEVGKIYITADENVQGAVFDLPEEIAKDLL 601

Query: 628 NKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRERSSRNSSRDRRGSRTSRGWGGSSR 687
             ++P GNTL KI+KLP LQDD P +DSYGRFS  +R SRN  R  RG   SRG GG   
Sbjct: 602 TMEVPPGNTLTKISKLPALQDDSPATDSYGRFS-NDRGSRN--RRSRGGGASRGRGGWDT 661

Query: 688 DSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIGGGSRRSSRSS--------------- 747
           DS+D        RG RS  S    +  S DDW   GG R+S+RSS               
Sbjct: 662 DSED-----RYRRGGRSLRSDNDSW--SDDDW--SGGGRKSNRSSSSFGGRSSSYGSRGS 721

Query: 748 ----------------SMDRFGGSCFNCGRMGHRASECPN 754
                           S   F G+CFNCG  GHRAS+CPN
Sbjct: 722 PSPSFGVRSSSLGGRESSRSFSGACFNCGESGHRASDCPN 744

BLAST of Cp4.1LG04g00220 vs. ExPASy Swiss-Prot
Match: Q9LUW5 (DEAD-box ATP-dependent RNA helicase 53, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RH53 PE=1 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.2e-130
Identity = 235/373 (63.00%), Postives = 294/373 (78.82%), Query Frame = 0

Query: 113 DELAISKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIP 172
           D LAIS+LG+   +  AL  +GI +LF IQ+AVL PA+EGRD+I RA+TGTGKTLAFGIP
Sbjct: 102 DGLAISELGISPEIVKALSSKGIEKLFPIQKAVLEPAMEGRDMIGRARTGTGKTLAFGIP 161

Query: 173 IIKKITEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVT 232
           II KI    +  +   R R P  LVL PTRELA+QVEKE +ESAP L+T+C+YGG     
Sbjct: 162 IIDKII---KYNAKHGRGRNPLCLVLAPTRELARQVEKEFRESAPSLDTICLYGGTPIGQ 221

Query: 233 QQSALSRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKL 292
           Q   L  GVDV VGTPGR+IDL+   +LNL EV+++VLDEADQML VGF EDVE+ILEKL
Sbjct: 222 QMRQLDYGVDVAVGTPGRVIDLMKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILEKL 281

Query: 293 PSQRQNMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTI 352
           P +RQ+M+FSATMP+W++ L +KYL+NPLT+DLVGD D+KLA+GI  Y+I+  +  + +I
Sbjct: 282 PEKRQSMMFSATMPSWIRSLTKKYLNNPLTVDLVGDSDQKLADGITTYSIIADSYGRASI 341

Query: 353 LRDLVTVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGK 412
           +  LVT +AKGGK IVFT+TKRDAD +S ALA +   EALHGDISQ QRERTL GFR G 
Sbjct: 342 IGPLVTEHAKGGKCIVFTQTKRDADRLSYALARSFKCEALHGDISQSQRERTLAGFRDGH 401

Query: 413 FTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRR 472
           F +LVATDVA+RGLD+PNVDL+IHYELPN+ ETFVHR+GRTGRAGK+G+AIL+++  Q R
Sbjct: 402 FNILVATDVAARGLDVPNVDLIIHYELPNNTETFVHRTGRTGRAGKKGSAILIYSQDQSR 461

Query: 473 TVRSLERDVGCKF 486
            V+ +ER+VG +F
Sbjct: 462 AVKIIEREVGSRF 471

BLAST of Cp4.1LG04g00220 vs. NCBI nr
Match: XP_023530437.1 (DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1444 bits (3738), Expect = 0.0
Identity = 754/754 (100.00%), Postives = 754/754 (100.00%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR
Sbjct: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60

Query: 61  NFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISKL 120
           NFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISKL
Sbjct: 61  NFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISKL 120

Query: 121 GLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITED 180
           GLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITED
Sbjct: 121 GLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITED 180

Query: 181 DELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRG 240
           DELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRG
Sbjct: 181 DELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRG 240

Query: 241 VDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNML 300
           VDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNML
Sbjct: 241 VDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNML 300

Query: 301 FSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVY 360
           FSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVY
Sbjct: 301 FSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVY 360

Query: 361 AKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATD 420
           AKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATD
Sbjct: 361 AKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATD 420

Query: 421 VASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERD 480
           VASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERD
Sbjct: 421 VASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERD 480

Query: 481 VGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALA 540
           VGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALA
Sbjct: 481 VGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALA 540

Query: 541 QLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKI 600
           QLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKI
Sbjct: 541 QLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKI 600

Query: 601 HLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRE 660
           HLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRE
Sbjct: 601 HLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRE 660

Query: 661 RSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIGG 720
           RSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIGG
Sbjct: 661 RSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIGG 720

Query: 721 GSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 754
           GSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN
Sbjct: 721 GSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 754

BLAST of Cp4.1LG04g00220 vs. NCBI nr
Match: XP_022931681.1 (DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1429 bits (3700), Expect = 0.0
Identity = 751/757 (99.21%), Postives = 752/757 (99.34%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATAT--VSSTPSLSFADKSHFNSLKALTS-LSSSS 60
           MASILGVTAIHHAPNLDMYRRMGTPATAT  VSSTPSLSFADKSHFNSLKALTS LSSSS
Sbjct: 1   MASILGVTAIHHAPNLDMYRRMGTPATATATVSSTPSLSFADKSHFNSLKALTSFLSSSS 60

Query: 61  SRRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAI 120
           SRRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAI
Sbjct: 61  SRRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAI 120

Query: 121 SKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKI 180
           SKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKI
Sbjct: 121 SKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKI 180

Query: 181 TEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSAL 240
           TEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSAL
Sbjct: 181 TEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSAL 240

Query: 241 SRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQ 300
           SRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQ
Sbjct: 241 SRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQ 300

Query: 301 NMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLV 360
           NMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLV
Sbjct: 301 NMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLV 360

Query: 361 TVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLV 420
           TVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLV
Sbjct: 361 TVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLV 420

Query: 421 ATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSL 480
           ATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTN+QRRTVRSL
Sbjct: 421 ATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNNQRRTVRSL 480

Query: 481 ERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAA 540
           ERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFF PTAQKLIDEQGAGALAA
Sbjct: 481 ERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFTPTAQKLIDEQGAGALAA 540

Query: 541 ALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEV 600
           ALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEV
Sbjct: 541 ALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEV 600

Query: 601 GKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFS 660
           GKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFS
Sbjct: 601 GKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFS 660

Query: 661 GRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWL 720
           GRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGR FKSSGDDWL
Sbjct: 661 GRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRNFKSSGDDWL 720

Query: 721 IGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 754
           IGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN
Sbjct: 721 IGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 757

BLAST of Cp4.1LG04g00220 vs. NCBI nr
Match: XP_022969384.1 (DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1428 bits (3696), Expect = 0.0
Identity = 747/756 (98.81%), Postives = 751/756 (99.34%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSS-- 60
           MASILGVTAIHHAPNLDMYRRMGTPATATVSS+PSLSFADKSHFNSLK+LTSLSSSSS  
Sbjct: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSSPSLSFADKSHFNSLKSLTSLSSSSSSS 60

Query: 61  RRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAIS 120
           RRN SGFSSSAIATPNSILSEEAFRSLDGFSKDSLDD+GLIDSEANSSLAFSDDDELAIS
Sbjct: 61  RRNLSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDNGLIDSEANSSLAFSDDDELAIS 120

Query: 121 KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT 180
           KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT
Sbjct: 121 KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT 180

Query: 181 EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240
           EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS
Sbjct: 181 EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240

Query: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300
           RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN
Sbjct: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300

Query: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360
           MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT
Sbjct: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360

Query: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420
           VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA
Sbjct: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420

Query: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480
           TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE
Sbjct: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480

Query: 481 RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAA 540
           RDVGCKFEFANPPGIEKVLESSAEQVV+TLRGVHPESIEFF PTAQKLIDEQGAGALAAA
Sbjct: 481 RDVGCKFEFANPPGIEKVLESSAEQVVITLRGVHPESIEFFTPTAQKLIDEQGAGALAAA 540

Query: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600
           LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG
Sbjct: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600

Query: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660
           KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG
Sbjct: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660

Query: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLI 720
           RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGR FKSSGDDWLI
Sbjct: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRNFKSSGDDWLI 720

Query: 721 GGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 754
           GGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN
Sbjct: 721 GGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 756

BLAST of Cp4.1LG04g00220 vs. NCBI nr
Match: KAG6588515.1 (DEAD-box ATP-dependent RNA helicase 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1384 bits (3583), Expect = 0.0
Identity = 729/735 (99.18%), Postives = 729/735 (99.18%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATAT--VSSTPSLSFADKSHFNSLKALTSLSSSSS 60
           MASILGVTAIHHAPNLDMYRRMGTPATAT  VSSTPSLSFADKSHFNS KALTSLSSS S
Sbjct: 1   MASILGVTAIHHAPNLDMYRRMGTPATATATVSSTPSLSFADKSHFNSFKALTSLSSSGS 60

Query: 61  RRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAIS 120
           RRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAIS
Sbjct: 61  RRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAIS 120

Query: 121 KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT 180
           KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT
Sbjct: 121 KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT 180

Query: 181 EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240
           EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS
Sbjct: 181 EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240

Query: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300
           RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN
Sbjct: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300

Query: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360
           MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT
Sbjct: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360

Query: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420
           VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA
Sbjct: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420

Query: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480
           TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE
Sbjct: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480

Query: 481 RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAA 540
           RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFF PTAQKLIDEQGAGALAAA
Sbjct: 481 RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFTPTAQKLIDEQGAGALAAA 540

Query: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600
           LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG
Sbjct: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600

Query: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660
           KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG
Sbjct: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660

Query: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLI 720
           RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGR FKSSGDDWLI
Sbjct: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRNFKSSGDDWLI 720

Query: 721 GGGSRRSSRSSSMDR 733
           GGGSRRSSRSSSMDR
Sbjct: 721 GGGSRRSSRSSSMDR 735

BLAST of Cp4.1LG04g00220 vs. NCBI nr
Match: XP_038879136.1 (DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Benincasa hispida])

HSP 1 Score: 1314 bits (3401), Expect = 0.0
Identity = 696/758 (91.82%), Postives = 724/758 (95.51%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MAS+LGVTAIHHAPNLDMYRRMGT  TAT S TPSLSFA+KSH  +LKA T  S SS RR
Sbjct: 1   MASLLGVTAIHHAPNLDMYRRMGT--TATASPTPSLSFAEKSHLIALKAQTCFSGSS-RR 60

Query: 61  NFSGF-SSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFS-----DDDE 120
           N SGF SSSAIATPNSILSEEAFRSLDGFSKDSLD + L+DSE+NSSLAFS     DDDE
Sbjct: 61  NLSGFTSSSAIATPNSILSEEAFRSLDGFSKDSLDGN-LVDSESNSSLAFSTAPPADDDE 120

Query: 121 LAISKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPII 180
           LAISKL LPQRL+DAL+KRGIT LF IQRAVLVPALEGRDLIARAKTGTGKTLAFGIPI+
Sbjct: 121 LAISKLNLPQRLTDALQKRGITHLFPIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIL 180

Query: 181 KKITEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQ 240
           KK+TEDDEL+S RRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSY+TQQ
Sbjct: 181 KKLTEDDELRSSRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYITQQ 240

Query: 241 SALSRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPS 300
           +ALSRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPS
Sbjct: 241 NALSRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPS 300

Query: 301 QRQNMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILR 360
           QRQ+MLFSATMPTWVKKLARKYL+NPLTIDLVGDQDEKLAEGIKL+AILTTATSKQTILR
Sbjct: 301 QRQSMLFSATMPTWVKKLARKYLENPLTIDLVGDQDEKLAEGIKLHAILTTATSKQTILR 360

Query: 361 DLVTVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFT 420
           DLVTVYAKGGKTIVFT+TKRDADEVSLALAN+ITSEALHGDISQHQRERTLNGFRQGKFT
Sbjct: 361 DLVTVYAKGGKTIVFTQTKRDADEVSLALANSITSEALHGDISQHQRERTLNGFRQGKFT 420

Query: 421 VLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTV 480
           VLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTV
Sbjct: 421 VLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTV 480

Query: 481 RSLERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGA 540
           RSLERDVGCKFEFANPP IE+VLESSAEQVVVTLRGVHPESIEFF PTA+KLIDEQG GA
Sbjct: 481 RSLERDVGCKFEFANPPAIEEVLESSAEQVVVTLRGVHPESIEFFTPTARKLIDEQGPGA 540

Query: 541 LAAALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAA 600
           LAAALAQLSGFS PPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSD+YSPAA
Sbjct: 541 LAAALAQLSGFSHPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDIYSPAA 600

Query: 601 DEVGKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYG 660
           DEVGKIHLIADERINGAVFDLPEEIAKELLNK+LPEGNT+LK+TKLPPLQDDGPPSD+YG
Sbjct: 601 DEVGKIHLIADERINGAVFDLPEEIAKELLNKELPEGNTILKVTKLPPLQDDGPPSDNYG 660

Query: 661 RFSGRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGD 720
           RFSGRER+SRNSSRDRRG RTSRGWG SSRDSDDDGDLFSRNR FR+NSSRGR FKSSGD
Sbjct: 661 RFSGRERNSRNSSRDRRGFRTSRGWG-SSRDSDDDGDLFSRNRSFRTNSSRGRNFKSSGD 720

Query: 721 DWLIGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECP 752
           DWLIGG  RRSSRSSS+DRFGGSCFNCGRMGHRASECP
Sbjct: 721 DWLIGG--RRSSRSSSVDRFGGSCFNCGRMGHRASECP 751

BLAST of Cp4.1LG04g00220 vs. ExPASy TrEMBL
Match: A0A6J1EUB8 (RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111437838 PE=3 SV=1)

HSP 1 Score: 1429 bits (3700), Expect = 0.0
Identity = 751/757 (99.21%), Postives = 752/757 (99.34%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATAT--VSSTPSLSFADKSHFNSLKALTS-LSSSS 60
           MASILGVTAIHHAPNLDMYRRMGTPATAT  VSSTPSLSFADKSHFNSLKALTS LSSSS
Sbjct: 1   MASILGVTAIHHAPNLDMYRRMGTPATATATVSSTPSLSFADKSHFNSLKALTSFLSSSS 60

Query: 61  SRRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAI 120
           SRRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAI
Sbjct: 61  SRRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAI 120

Query: 121 SKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKI 180
           SKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKI
Sbjct: 121 SKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKI 180

Query: 181 TEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSAL 240
           TEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSAL
Sbjct: 181 TEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSAL 240

Query: 241 SRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQ 300
           SRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQ
Sbjct: 241 SRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQ 300

Query: 301 NMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLV 360
           NMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLV
Sbjct: 301 NMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLV 360

Query: 361 TVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLV 420
           TVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLV
Sbjct: 361 TVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLV 420

Query: 421 ATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSL 480
           ATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTN+QRRTVRSL
Sbjct: 421 ATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNNQRRTVRSL 480

Query: 481 ERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAA 540
           ERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFF PTAQKLIDEQGAGALAA
Sbjct: 481 ERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFTPTAQKLIDEQGAGALAA 540

Query: 541 ALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEV 600
           ALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEV
Sbjct: 541 ALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEV 600

Query: 601 GKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFS 660
           GKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFS
Sbjct: 601 GKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFS 660

Query: 661 GRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWL 720
           GRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGR FKSSGDDWL
Sbjct: 661 GRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRNFKSSGDDWL 720

Query: 721 IGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 754
           IGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN
Sbjct: 721 IGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 757

BLAST of Cp4.1LG04g00220 vs. ExPASy TrEMBL
Match: A0A6J1I2F6 (RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111468402 PE=3 SV=1)

HSP 1 Score: 1428 bits (3696), Expect = 0.0
Identity = 747/756 (98.81%), Postives = 751/756 (99.34%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSS-- 60
           MASILGVTAIHHAPNLDMYRRMGTPATATVSS+PSLSFADKSHFNSLK+LTSLSSSSS  
Sbjct: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSSPSLSFADKSHFNSLKSLTSLSSSSSSS 60

Query: 61  RRNFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAIS 120
           RRN SGFSSSAIATPNSILSEEAFRSLDGFSKDSLDD+GLIDSEANSSLAFSDDDELAIS
Sbjct: 61  RRNLSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDNGLIDSEANSSLAFSDDDELAIS 120

Query: 121 KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT 180
           KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT
Sbjct: 121 KLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKIT 180

Query: 181 EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240
           EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS
Sbjct: 181 EDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240

Query: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300
           RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN
Sbjct: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300

Query: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360
           MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT
Sbjct: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360

Query: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420
           VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA
Sbjct: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420

Query: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480
           TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE
Sbjct: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480

Query: 481 RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAA 540
           RDVGCKFEFANPPGIEKVLESSAEQVV+TLRGVHPESIEFF PTAQKLIDEQGAGALAAA
Sbjct: 481 RDVGCKFEFANPPGIEKVLESSAEQVVITLRGVHPESIEFFTPTAQKLIDEQGAGALAAA 540

Query: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600
           LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG
Sbjct: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600

Query: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660
           KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG
Sbjct: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660

Query: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLI 720
           RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGR FKSSGDDWLI
Sbjct: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRNFKSSGDDWLI 720

Query: 721 GGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 754
           GGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN
Sbjct: 721 GGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPNN 756

BLAST of Cp4.1LG04g00220 vs. ExPASy TrEMBL
Match: A0A1S3BQ06 (RNA helicase OS=Cucumis melo OX=3656 GN=LOC103492086 PE=3 SV=1)

HSP 1 Score: 1312 bits (3395), Expect = 0.0
Identity = 690/753 (91.63%), Postives = 719/753 (95.48%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MAS+LGVTAIHHAPNLD+YRRMGT  TAT S TPSLSF DKSH  +LK  T  S SS RR
Sbjct: 1   MASLLGVTAIHHAPNLDLYRRMGT--TATASPTPSLSFVDKSHLIALKVQTCFSGSS-RR 60

Query: 61  NFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFS-DDDELAISK 120
           N SGF+SSAIATPNSILSEEAFRSLDGFSKD LDD+ LID E NSSLAFS DDDELAISK
Sbjct: 61  NLSGFTSSAIATPNSILSEEAFRSLDGFSKDPLDDN-LIDPEPNSSLAFSADDDELAISK 120

Query: 121 LGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITE 180
           L LPQRL+DAL+KRGIT LF IQRAVLVPALEGRDLIARAKTGTGKTLAFGIPI+KK+TE
Sbjct: 121 LNLPQRLTDALQKRGITHLFPIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPILKKLTE 180

Query: 181 DDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSR 240
           DDE +S RRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQ+ALSR
Sbjct: 181 DDESRSLRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQNALSR 240

Query: 241 GVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNM 300
           GVDVVVGTPGR+IDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNM
Sbjct: 241 GVDVVVGTPGRLIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNM 300

Query: 301 LFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTV 360
           LFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKL+AILTTATSKQTILRDLVTV
Sbjct: 301 LFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLHAILTTATSKQTILRDLVTV 360

Query: 361 YAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVAT 420
           YAKGGKTIVFT+TKRDADEVSLALAN+ITSEALHGDISQHQRERTLNGFRQGKFTVLVAT
Sbjct: 361 YAKGGKTIVFTQTKRDADEVSLALANSITSEALHGDISQHQRERTLNGFRQGKFTVLVAT 420

Query: 421 DVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLER 480
           DVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLER
Sbjct: 421 DVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLER 480

Query: 481 DVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAAL 540
           DVGCKFEFANPP IE+VLESSAEQVVVTLRGVHPESIE+F PTAQKLIDEQG GALAAAL
Sbjct: 481 DVGCKFEFANPPAIEQVLESSAEQVVVTLRGVHPESIEYFTPTAQKLIDEQGPGALAAAL 540

Query: 541 AQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGK 600
           AQLSGFS PPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSD+YSPAADE+GK
Sbjct: 541 AQLSGFSHPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDIYSPAADEIGK 600

Query: 601 IHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGR 660
           IHLIADERINGAVFDLPEEIAKELLNK+LPEGNT+LK+TKLPPLQDDGPPSD+YGRFSGR
Sbjct: 601 IHLIADERINGAVFDLPEEIAKELLNKELPEGNTILKVTKLPPLQDDGPPSDNYGRFSGR 660

Query: 661 ERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIG 720
           ER+SRNSSRDRRG RTSRGWG SSRDSDDDGD+FSRNR FR+NSS+GR F+SSGDDWLIG
Sbjct: 661 ERNSRNSSRDRRGFRTSRGWG-SSRDSDDDGDMFSRNRSFRTNSSKGRNFRSSGDDWLIG 720

Query: 721 GGSRRSSRSSSMDRFGGSCFNCGRMGHRASECP 752
           G  RRSSRSSS+DRFGGSCFNCGRMGHRASECP
Sbjct: 721 G--RRSSRSSSVDRFGGSCFNCGRMGHRASECP 746

BLAST of Cp4.1LG04g00220 vs. ExPASy TrEMBL
Match: A0A0A0M1E9 (RNA helicase OS=Cucumis sativus OX=3659 GN=Csa_1G560830 PE=3 SV=1)

HSP 1 Score: 1299 bits (3361), Expect = 0.0
Identity = 683/753 (90.70%), Postives = 718/753 (95.35%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MAS+LGVTAI HAPNLD+YRR GT  TAT S TPSLSF DKSH  +LK  T  S SS RR
Sbjct: 1   MASLLGVTAILHAPNLDLYRRTGT--TATASPTPSLSFVDKSHLIALKVQTCFSGSS-RR 60

Query: 61  NFSGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFS-DDDELAISK 120
           N SGF+SSAIATPNSILSEEAFRS DGFS+DSLDD+ LID E NSSLAF+ DDDELAISK
Sbjct: 61  NLSGFTSSAIATPNSILSEEAFRSFDGFSEDSLDDN-LIDPEPNSSLAFAADDDELAISK 120

Query: 121 LGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITE 180
           L LPQRL+DAL+KRGIT LF IQRAVLVPALEGRDLIARAKTGTGKTLAFGIPI+KK+TE
Sbjct: 121 LNLPQRLTDALQKRGITHLFPIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPILKKLTE 180

Query: 181 DDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSR 240
           DDE +S RRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSY+TQQ+ALSR
Sbjct: 181 DDESRSLRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYITQQNALSR 240

Query: 241 GVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNM 300
           GVDVVVGTPGR+IDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNM
Sbjct: 241 GVDVVVGTPGRLIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNM 300

Query: 301 LFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTV 360
           LFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKL+AILTTATSKQTILRDLVTV
Sbjct: 301 LFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLHAILTTATSKQTILRDLVTV 360

Query: 361 YAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVAT 420
           YAKGGKTIVFT+TKRDADEVSLALAN+ITSEALHGDISQHQRERTLNGFRQGKFTVLVAT
Sbjct: 361 YAKGGKTIVFTQTKRDADEVSLALANSITSEALHGDISQHQRERTLNGFRQGKFTVLVAT 420

Query: 421 DVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLER 480
           DVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLER
Sbjct: 421 DVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLER 480

Query: 481 DVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAAL 540
           DVGCKFEFANPPG+E+VL+SSAEQVVVTLRGVHPESIE+F PTAQKLIDEQG GALAAAL
Sbjct: 481 DVGCKFEFANPPGMEEVLKSSAEQVVVTLRGVHPESIEYFTPTAQKLIDEQGLGALAAAL 540

Query: 541 AQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGK 600
           AQLSGF+ PPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADE+GK
Sbjct: 541 AQLSGFTHPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEIGK 600

Query: 601 IHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGR 660
           IHLIADERINGAVFDLPEEIAKELLNK+LPEGNT+LKITKLPPLQDDGPPSD+YGRFSGR
Sbjct: 601 IHLIADERINGAVFDLPEEIAKELLNKELPEGNTILKITKLPPLQDDGPPSDNYGRFSGR 660

Query: 661 ERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIG 720
           ERSSRNSSRDRRG +TSRGWG SSRDSDD+GD+FSRNR FR+N+S+GR F+SSGDDWLIG
Sbjct: 661 ERSSRNSSRDRRGLKTSRGWG-SSRDSDDNGDIFSRNRSFRTNNSKGRNFRSSGDDWLIG 720

Query: 721 GGSRRSSRSSSMDRFGGSCFNCGRMGHRASECP 752
           G  RRSSRSSS+DRFGGSCFNCGRMGHRASECP
Sbjct: 721 G--RRSSRSSSVDRFGGSCFNCGRMGHRASECP 746

BLAST of Cp4.1LG04g00220 vs. ExPASy TrEMBL
Match: A0A6J1DUC8 (RNA helicase OS=Momordica charantia OX=3673 GN=LOC111024523 PE=3 SV=1)

HSP 1 Score: 1280 bits (3312), Expect = 0.0
Identity = 684/770 (88.83%), Postives = 712/770 (92.47%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MASILGVTAIHHAPNLD+YRRMGT AT   S TPSLSFADKSH N+ KA TS SS S RR
Sbjct: 1   MASILGVTAIHHAPNLDIYRRMGTAATP--SPTPSLSFADKSHINASKAQTSFSSGS-RR 60

Query: 61  NF------------SGFSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLA 120
           N             SGFSSSAIATPNSILSEEAF+SLDGFSKD       IDS+ NSSL 
Sbjct: 61  NLAFSDGSSAGTFKSGFSSSAIATPNSILSEEAFKSLDGFSKDDF-----IDSQPNSSLG 120

Query: 121 FS-----DDDELAISKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGT 180
           FS     D+DELAISKLGLPQRL+++LEKRGIT LF IQRAVLVPALEGRDLIARAKTGT
Sbjct: 121 FSADSPADEDELAISKLGLPQRLTESLEKRGITSLFPIQRAVLVPALEGRDLIARAKTGT 180

Query: 181 GKTLAFGIPIIKKITEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVC 240
           GKTLAFGIPIIK++TEDDEL+S RRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVC
Sbjct: 181 GKTLAFGIPIIKRLTEDDELRSSRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVC 240

Query: 241 VYGGVSYVTQQSALSRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEE 300
           VYGGVSYVTQQ+AL+RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEE
Sbjct: 241 VYGGVSYVTQQNALTRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEE 300

Query: 301 DVEVILEKLPSQRQNMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAIL 360
           DVEVIL+KLP+QRQNMLFSATMPTWVKKLARKYL+NPLTIDLVG QDEKLAEGIKLYAIL
Sbjct: 301 DVEVILQKLPTQRQNMLFSATMPTWVKKLARKYLENPLTIDLVGXQDEKLAEGIKLYAIL 360

Query: 361 TTATSKQTILRDLVTVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRER 420
           TTATSKQTILRDLVTVYAKGGKTIVFT+TKRDADEVSLALAN+ITSEALHGDISQHQRER
Sbjct: 361 TTATSKQTILRDLVTVYAKGGKTIVFTQTKRDADEVSLALANSITSEALHGDISQHQRER 420

Query: 421 TLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAI 480
           TLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEG+AI
Sbjct: 421 TLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGSAI 480

Query: 481 LMFTNSQRRTVRSLERDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTA 540
           LMFTNSQRRTVRSLERDVGCKFEFANPP IE VLESSAEQVV TLRGVHPESIEFF PTA
Sbjct: 481 LMFTNSQRRTVRSLERDVGCKFEFANPPAIEDVLESSAEQVVATLRGVHPESIEFFTPTA 540

Query: 541 QKLIDEQGAGALAAALAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVT 600
           QKLIDEQGAGALAAALAQLSGFS PPSSRSLI+HEQGWVTLQLTRDPSYSRGFLSARSVT
Sbjct: 541 QKLIDEQGAGALAAALAQLSGFSHPPSSRSLISHEQGWVTLQLTRDPSYSRGFLSARSVT 600

Query: 601 GFLSDVYSPAADEVGKIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPL 660
           GFLSD+YSPAADEVGKIHLIADERI+GAVFDLPEEIAKELLNK LPEGNT+LK+TKLPPL
Sbjct: 601 GFLSDIYSPAADEVGKIHLIADERIHGAVFDLPEEIAKELLNKPLPEGNTILKVTKLPPL 660

Query: 661 QDDGPPSDSYGRFSGRERSSRNSSRDRRGSRTSRGWGGSSRDSDDDGDLFSRNRGFRSN- 720
           QDDGPPSD+YGRFSGRER+SRN+SRDRRG RTSRGWG S RDSDDDGDLFSRNR FR+N 
Sbjct: 661 QDDGPPSDNYGRFSGRERNSRNNSRDRRGFRTSRGWG-SGRDSDDDGDLFSRNRSFRTNN 720

Query: 721 SSRGRKFKSSGDDWLIGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECP 752
           SSR R FKSSGDDWLIGG  RRSSRSSSMD FGGSCFNCGRMGHRASECP
Sbjct: 721 SSRSRNFKSSGDDWLIGG--RRSSRSSSMDGFGGSCFNCGRMGHRASECP 759

BLAST of Cp4.1LG04g00220 vs. TAIR 10
Match: AT5G26742.1 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 969.5 bits (2505), Expect = 1.5e-282
Identity = 538/758 (70.98%), Postives = 610/758 (80.47%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MAS +GV +++  P+L++ +    P +   S+  SLS  DK  F  L    SL   + R 
Sbjct: 1   MASTVGVPSLYQVPHLEISK----PNSKKRSNCLSLSL-DKPFFTPL----SLVRRTRRI 60

Query: 61  NFSG-FSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISK 120
           + S     SA+ATPNS+LSEEAF+SL     D  D DG      N+++   D +ELAISK
Sbjct: 61  HSSSLLVPSAVATPNSVLSEEAFKSLGLSDHDEYDLDG-----DNNNVEADDGEELAISK 120

Query: 121 LGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITE 180
           L LPQRL ++LEKRGIT LF IQRAVLVPAL+GRD+IARAKTGTGKTLAFGIPIIK++TE
Sbjct: 121 LSLPQRLEESLEKRGITHLFPIQRAVLVPALQGRDIIARAKTGTGKTLAFGIPIIKRLTE 180

Query: 181 D-DELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240
           +  +  + RR  RLP+ LVL PTRELAKQVEKEIKESAPYL+TVCVYGGVSY  QQSAL+
Sbjct: 181 EAGDYTAFRRSGRLPKFLVLAPTRELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQSALT 240

Query: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300
           RGVDVVVGTPGRIIDLI G SL LGEVEYLVLDEADQMLAVGFEE VE ILE LP++RQ+
Sbjct: 241 RGVDVVVGTPGRIIDLIEGRSLKLGEVEYLVLDEADQMLAVGFEEAVESILENLPTKRQS 300

Query: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360
           MLFSATMPTWVKKLARKYLDNPL IDLVGDQDEKLAEGIKLYAI TT+TSK+TIL DL+T
Sbjct: 301 MLFSATMPTWVKKLARKYLDNPLNIDLVGDQDEKLAEGIKLYAIATTSTSKRTILSDLIT 360

Query: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420
           VYAKGGKTIVFT+TKRDADEVSLAL+N+I +EALHGDISQHQRERTLN FRQGKFTVLVA
Sbjct: 361 VYAKGGKTIVFTQTKRDADEVSLALSNSIATEALHGDISQHQRERTLNAFRQGKFTVLVA 420

Query: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480
           TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEG+AILM T+SQ+RTVRSLE
Sbjct: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGSAILMHTSSQKRTVRSLE 480

Query: 481 RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAA 540
           RDVGC FEF +PP +  +LESSA+QVV TL GVHP+SI+FF  TAQKL +E+G  ALAAA
Sbjct: 481 RDVGCHFEFISPPTVGDLLESSADQVVATLNGVHPDSIKFFSATAQKLYEEKGTDALAAA 540

Query: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600
           LA LSGFS+PPSSRSL++HE+GWVTLQL RDP+ +RGFLSARSVTGFLSD+Y  AADEVG
Sbjct: 541 LAHLSGFSQPPSSRSLLSHEKGWVTLQLIRDPTNARGFLSARSVTGFLSDLYRTAADEVG 600

Query: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660
           KI LIAD+RI GAVFDLPEEIAKELL KD+PEGN+L  ITKLPPLQDDGP SD+YGRFS 
Sbjct: 601 KIFLIADDRIQGAVFDLPEEIAKELLEKDVPEGNSLSMITKLPPLQDDGPSSDNYGRFSS 660

Query: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDS---DDDGDLFSRNRGFRSNSSRGRKFKSSGDD 720
           R+R  R      RGSR  RG     RDS   DDD      + G  S S  G   + S DD
Sbjct: 661 RDRMPRGGG-GSRGSRGGRGGSSRGRDSWGGDDDRGSRRSSGGGSSWSRGGSSSRGSSDD 720

Query: 721 WLIGGGSRRSSRSSSMDRFGGSCFNCGRMGHRASECPN 754
           WLIGG S  SSR+ S + FGGSCF CG+ GHRA++CP+
Sbjct: 721 WLIGGRSSSSSRAPSRESFGGSCFICGKSGHRATDCPD 743

BLAST of Cp4.1LG04g00220 vs. TAIR 10
Match: AT5G26742.2 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 967.2 bits (2499), Expect = 7.5e-282
Identity = 539/759 (71.01%), Postives = 611/759 (80.50%), Query Frame = 0

Query: 1   MASILGVTAIHHAPNLDMYRRMGTPATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRR 60
           MAS +GV +++  P+L++ +    P +   S+  SLS  DK  F  L    SL   + R 
Sbjct: 1   MASTVGVPSLYQVPHLEISK----PNSKKRSNCLSLSL-DKPFFTPL----SLVRRTRRI 60

Query: 61  NFSG-FSSSAIATPNSILSEEAFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISK 120
           + S     SA+ATPNS+LSEEAF+SL     D  D DG      N+++   D +ELAISK
Sbjct: 61  HSSSLLVPSAVATPNSVLSEEAFKSLGLSDHDEYDLDG-----DNNNVEADDGEELAISK 120

Query: 121 LGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITE 180
           L LPQRL ++LEKRGIT LF IQRAVLVPAL+GRD+IARAKTGTGKTLAFGIPIIK++TE
Sbjct: 121 LSLPQRLEESLEKRGITHLFPIQRAVLVPALQGRDIIARAKTGTGKTLAFGIPIIKRLTE 180

Query: 181 D-DELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALS 240
           +  +  + RR  RLP+ LVL PTRELAKQVEKEIKESAPYL+TVCVYGGVSY  QQSAL+
Sbjct: 181 EAGDYTAFRRSGRLPKFLVLAPTRELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQSALT 240

Query: 241 RGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQN 300
           RGVDVVVGTPGRIIDLI G SL LGEVEYLVLDEADQMLAVGFEE VE ILE LP++RQ+
Sbjct: 241 RGVDVVVGTPGRIIDLIEGRSLKLGEVEYLVLDEADQMLAVGFEEAVESILENLPTKRQS 300

Query: 301 MLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVT 360
           MLFSATMPTWVKKLARKYLDNPL IDLVGDQDEKLAEGIKLYAI TT+TSK+TIL DL+T
Sbjct: 301 MLFSATMPTWVKKLARKYLDNPLNIDLVGDQDEKLAEGIKLYAIATTSTSKRTILSDLIT 360

Query: 361 VYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVA 420
           VYAKGGKTIVFT+TKRDADEVSLAL+N+I +EALHGDISQHQRERTLN FRQGKFTVLVA
Sbjct: 361 VYAKGGKTIVFTQTKRDADEVSLALSNSIATEALHGDISQHQRERTLNAFRQGKFTVLVA 420

Query: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLE 480
           TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEG+AILM T+SQ+RTVRSLE
Sbjct: 421 TDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGSAILMHTSSQKRTVRSLE 480

Query: 481 RDVGCKFEFANPPGIEKVLESSAEQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAA 540
           RDVGC FEF +PP +  +LESSA+QVV TL GVHP+SI+FF  TAQKL +E+G  ALAAA
Sbjct: 481 RDVGCHFEFISPPTVGDLLESSADQVVATLNGVHPDSIKFFSATAQKLYEEKGTDALAAA 540

Query: 541 LAQLSGFSRPPSSRSLINHEQGWVTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVG 600
           LA LSGFS+PPSSRSL++HE+GWVTLQL RDP+ +RGFLSARSVTGFLSD+Y  AADEVG
Sbjct: 541 LAHLSGFSQPPSSRSLLSHEKGWVTLQLIRDPTNARGFLSARSVTGFLSDLYRTAADEVG 600

Query: 601 KIHLIADERINGAVFDLPEEIAKELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSG 660
           KI LIAD+RI GAVFDLPEEIAKELL KD+PEGN+L  ITKLPPLQDDGP SD+YGRFS 
Sbjct: 601 KIFLIADDRIQGAVFDLPEEIAKELLEKDVPEGNSLSMITKLPPLQDDGPSSDNYGRFSS 660

Query: 661 RERSSRNSSRDRRGSRTSRGWGGSSRDS---DDDGDLFSRNRGFRSNSSRGRKFKSSGDD 720
           R+R  R      RGSR  RG     RDS   DDD      + G  S S  G   + S DD
Sbjct: 661 RDRMPRGGG-GSRGSRGGRGGSSRGRDSWGGDDDRGSRRSSGGGSSWSRGGSSSRGSSDD 720

Query: 721 WLIGGGSRRSSRSSSMDR-FGGSCFNCGRMGHRASECPN 754
           WLIGG S  SSR+ S +R FGGSCF CG+ GHRA++CP+
Sbjct: 721 WLIGGRSSSSSRAPSRERSFGGSCFICGKSGHRATDCPD 744

BLAST of Cp4.1LG04g00220 vs. TAIR 10
Match: AT5G26742.3 (DEAD box RNA helicase (RH3) )

HSP 1 Score: 893.6 bits (2308), Expect = 1.1e-259
Identity = 475/616 (77.11%), Postives = 526/616 (85.39%), Query Frame = 0

Query: 143 RAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITED-DELKSPRRRSRLPRVLVLTPT 202
           RAVLVPAL+GRD+IARAKTGTGKTLAFGIPIIK++TE+  +  + RR  RLP+ LVL PT
Sbjct: 37  RAVLVPALQGRDIIARAKTGTGKTLAFGIPIIKRLTEEAGDYTAFRRSGRLPKFLVLAPT 96

Query: 203 RELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRGVDVVVGTPGRIIDLINGNSLN 262
           RELAKQVEKEIKESAPYL+TVCVYGGVSY  QQSAL+RGVDVVVGTPGRIIDLI G SL 
Sbjct: 97  RELAKQVEKEIKESAPYLSTVCVYGGVSYTIQQSALTRGVDVVVGTPGRIIDLIEGRSLK 156

Query: 263 LGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNMLFSATMPTWVKKLARKYLDNPL 322
           LGEVEYLVLDEADQMLAVGFEE VE ILE LP++RQ+MLFSATMPTWVKKLARKYLDNPL
Sbjct: 157 LGEVEYLVLDEADQMLAVGFEEAVESILENLPTKRQSMLFSATMPTWVKKLARKYLDNPL 216

Query: 323 TIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVYAKGGKTIVFTRTKRDADEVSL 382
            IDLVGDQDEKLAEGIKLYAI TT+TSK+TIL DL+TVYAKGGKTIVFT+TKRDADEVSL
Sbjct: 217 NIDLVGDQDEKLAEGIKLYAIATTSTSKRTILSDLITVYAKGGKTIVFTQTKRDADEVSL 276

Query: 383 ALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPN 442
           AL+N+I +EALHGDISQHQRERTLN FRQGKFTVLVATDVASRGLDIPNVDLVIHYELPN
Sbjct: 277 ALSNSIATEALHGDISQHQRERTLNAFRQGKFTVLVATDVASRGLDIPNVDLVIHYELPN 336

Query: 443 DPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERDVGCKFEFANPPGIEKVLESSA 502
           DPETFVHRSGRTGRAGKEG+AILM T+SQ+RTVRSLERDVGC FEF +PP +  +LESSA
Sbjct: 337 DPETFVHRSGRTGRAGKEGSAILMHTSSQKRTVRSLERDVGCHFEFISPPTVGDLLESSA 396

Query: 503 EQVVVTLRGVHPESIEFFIPTAQKLIDEQGAGALAAALAQLSGFSRPPSSRSLINHEQGW 562
           +QVV TL GVHP+SI+FF  TAQKL +E+G  ALAAALA LSGFS+PPSSRSL++HE+GW
Sbjct: 397 DQVVATLNGVHPDSIKFFSATAQKLYEEKGTDALAAALAHLSGFSQPPSSRSLLSHEKGW 456

Query: 563 VTLQLTRDPSYSRGFLSARSVTGFLSDVYSPAADEVGKIHLIADERINGAVFDLPEEIAK 622
           VTLQL RDP+ +RGFLSARSVTGFLSD+Y  AADEVGKI LIAD+RI GAVFDLPEEIAK
Sbjct: 457 VTLQLIRDPTNARGFLSARSVTGFLSDLYRTAADEVGKIFLIADDRIQGAVFDLPEEIAK 516

Query: 623 ELLNKDLPEGNTLLKITKLPPLQDDGPPSDSYGRFSGRERSSRNSSRDRRGSRTSRGWGG 682
           ELL KD+PEGN+L  ITKLPPLQDDGP SD+YGRFS R+R  R      RGSR  RG   
Sbjct: 517 ELLEKDVPEGNSLSMITKLPPLQDDGPSSDNYGRFSSRDRMPRGGG-GSRGSRGGRGGSS 576

Query: 683 SSRDS---DDDGDLFSRNRGFRSNSSRGRKFKSSGDDWLIGGGSRRSSRSSSMDR-FGGS 742
             RDS   DDD      + G  S S  G   + S DDWLIGG S  SSR+ S +R FGGS
Sbjct: 577 RGRDSWGGDDDRGSRRSSGGGSSWSRGGSSSRGSSDDWLIGGRSSSSSRAPSRERSFGGS 636

Query: 743 CFNCGRMGHRASECPN 754
           CF CG+ GHRA++CP+
Sbjct: 637 CFICGKSGHRATDCPD 651

BLAST of Cp4.1LG04g00220 vs. TAIR 10
Match: AT3G22330.1 (putative mitochondrial RNA helicase 2 )

HSP 1 Score: 468.8 bits (1205), Expect = 8.4e-132
Identity = 235/373 (63.00%), Postives = 294/373 (78.82%), Query Frame = 0

Query: 113 DELAISKLGLPQRLSDALEKRGITQLFAIQRAVLVPALEGRDLIARAKTGTGKTLAFGIP 172
           D LAIS+LG+   +  AL  +GI +LF IQ+AVL PA+EGRD+I RA+TGTGKTLAFGIP
Sbjct: 102 DGLAISELGISPEIVKALSSKGIEKLFPIQKAVLEPAMEGRDMIGRARTGTGKTLAFGIP 161

Query: 173 IIKKITEDDELKSPRRRSRLPRVLVLTPTRELAKQVEKEIKESAPYLNTVCVYGGVSYVT 232
           II KI    +  +   R R P  LVL PTRELA+QVEKE +ESAP L+T+C+YGG     
Sbjct: 162 IIDKII---KYNAKHGRGRNPLCLVLAPTRELARQVEKEFRESAPSLDTICLYGGTPIGQ 221

Query: 233 QQSALSRGVDVVVGTPGRIIDLINGNSLNLGEVEYLVLDEADQMLAVGFEEDVEVILEKL 292
           Q   L  GVDV VGTPGR+IDL+   +LNL EV+++VLDEADQML VGF EDVE+ILEKL
Sbjct: 222 QMRQLDYGVDVAVGTPGRVIDLMKRGALNLSEVQFVVLDEADQMLQVGFAEDVEIILEKL 281

Query: 293 PSQRQNMLFSATMPTWVKKLARKYLDNPLTIDLVGDQDEKLAEGIKLYAILTTATSKQTI 352
           P +RQ+M+FSATMP+W++ L +KYL+NPLT+DLVGD D+KLA+GI  Y+I+  +  + +I
Sbjct: 282 PEKRQSMMFSATMPSWIRSLTKKYLNNPLTVDLVGDSDQKLADGITTYSIIADSYGRASI 341

Query: 353 LRDLVTVYAKGGKTIVFTRTKRDADEVSLALANTITSEALHGDISQHQRERTLNGFRQGK 412
           +  LVT +AKGGK IVFT+TKRDAD +S ALA +   EALHGDISQ QRERTL GFR G 
Sbjct: 342 IGPLVTEHAKGGKCIVFTQTKRDADRLSYALARSFKCEALHGDISQSQRERTLAGFRDGH 401

Query: 413 FTVLVATDVASRGLDIPNVDLVIHYELPNDPETFVHRSGRTGRAGKEGTAILMFTNSQRR 472
           F +LVATDVA+RGLD+PNVDL+IHYELPN+ ETFVHR+GRTGRAGK+G+AIL+++  Q R
Sbjct: 402 FNILVATDVAARGLDVPNVDLIIHYELPNNTETFVHRTGRTGRAGKKGSAILIYSQDQSR 461

Query: 473 TVRSLERDVGCKF 486
            V+ +ER+VG +F
Sbjct: 462 AVKIIEREVGSRF 471

BLAST of Cp4.1LG04g00220 vs. TAIR 10
Match: AT3G22310.1 (putative mitochondrial RNA helicase 1 )

HSP 1 Score: 455.7 bits (1171), Expect = 7.3e-128
Identity = 257/484 (53.10%), Postives = 334/484 (69.01%), Query Frame = 0

Query: 25  PATATVSSTPSLSFADKSHFNSLKALTSLSSSSSRRNFSGFSSSAIATP----NSILSEE 84
           PA ATVS             N    + SL S+SS            + P    +SI+S  
Sbjct: 35  PAAATVSDLA----------NGATNVKSLPSNSSPFGVKVRDFHVKSVPSEFRSSIVSSA 94

Query: 85  AFRSLDGFSKDSLDDDGLIDSEANSSLAFSDDDELAISKLGLPQRLSDALEKRGITQLFA 144
            F + + ++    +D G+ DSE+  S      D LAI+ LG+   +  AL+ RGI +LF 
Sbjct: 95  GFAAQE-YAPSYENDGGIGDSESVGS--SGGGDGLAIADLGISPEIVKALKGRGIEKLFP 154

Query: 145 IQRAVLVPALEGRDLIARAKTGTGKTLAFGIPIIKKITEDDELKSPRRRSRLPRVLVLTP 204
           IQ+AVL PA+EGRD+I RA+TGTGKTLAFGIPII KI    +  +   R + P+ LVL P
Sbjct: 155 IQKAVLEPAMEGRDMIGRARTGTGKTLAFGIPIIDKII---KFNAKHGRGKNPQCLVLAP 214

Query: 205 TRELAKQVEKEIKESAPYLNTVCVYGGVSYVTQQSALSRGVDVVVGTPGRIIDLINGNSL 264
           TRELA+QVEKE +ESAP L+T+C+YGG     Q   L+ G+DV VGTPGRIIDL+   +L
Sbjct: 215 TRELARQVEKEFRESAPSLDTICLYGGTPIGQQMRELNYGIDVAVGTPGRIIDLMKRGAL 274

Query: 265 NLGEVEYLVLDEADQMLAVGFEEDVEVILEKLPSQRQNMLFSATMPTWVKKLARKYLDNP 324
           NL EV+++VLDEADQML VGF EDVE+IL+KLP++RQ+M+FSATMP+W++ L +KYL+NP
Sbjct: 275 NLSEVQFVVLDEADQMLQVGFAEDVEIILQKLPAKRQSMMFSATMPSWIRSLTKKYLNNP 334

Query: 325 LTIDLVGDQDEKLAEGIKLYAILTTATSKQTILRDLVTVYAKGGKTIVFTRTKRDADEVS 384
           LTIDLVGD D+KLA+GI +Y+I   +  + +I+  LV  + KGGK IVFT+TKRDAD ++
Sbjct: 335 LTIDLVGDSDQKLADGITMYSIAADSYGRASIIGPLVKEHGKGGKCIVFTQTKRDADRLA 394

Query: 385 LALANTITSEALHGDISQHQRERTLNGFRQGKFTVLVATDVASRGLDIPNVDLVIHYELP 444
             LA +   EALHGDISQ QRERTL GFR G F++LVATDVA+RGLD+PNVDLVIHYELP
Sbjct: 395 FGLAKSYKCEALHGDISQAQRERTLAGFRDGNFSILVATDVAARGLDVPNVDLVIHYELP 454

Query: 445 NDPETFVHRSGRTGRAGKEGTAILMFTNSQRRTVRSLERDVGCKFEFANPPGIEKVLESS 504
           N+ ETFVHR+GRTGRAGK+G+AIL+    Q R V+ +E++VG +F       +E+   S 
Sbjct: 455 NNTETFVHRTGRTGRAGKKGSAILIHGQDQTRAVKMIEKEVGSRFNELPSIAVERGSASM 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7S81.1e-28071.01DEAD-box ATP-dependent RNA helicase 3, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q0DM515.1e-26767.82DEAD-box ATP-dependent RNA helicase 3, chloroplastic OS=Oryza sativa subsp. japo... [more]
A0A1D6LAB72.0e-26369.50DEAD-box ATP-dependent RNA helicase 3B, chloroplastic OS=Zea mays OX=4577 GN=RH3... [more]
A0A1D6GDY88.4e-26266.71DEAD-box ATP-dependent RNA helicase 3A, chloroplastic OS=Zea mays OX=4577 GN=RH3... [more]
Q9LUW51.2e-13063.00DEAD-box ATP-dependent RNA helicase 53, mitochondrial OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_023530437.10.0100.00DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Cucurbita pepo subsp. pepo... [more]
XP_022931681.10.099.21DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Cucurbita moschata][more]
XP_022969384.10.098.81DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Cucurbita maxima][more]
KAG6588515.10.099.18DEAD-box ATP-dependent RNA helicase 3, chloroplastic, partial [Cucurbita argyros... [more]
XP_038879136.10.091.82DEAD-box ATP-dependent RNA helicase 3, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1EUB80.099.21RNA helicase OS=Cucurbita moschata OX=3662 GN=LOC111437838 PE=3 SV=1[more]
A0A6J1I2F60.098.81RNA helicase OS=Cucurbita maxima OX=3661 GN=LOC111468402 PE=3 SV=1[more]
A0A1S3BQ060.091.63RNA helicase OS=Cucumis melo OX=3656 GN=LOC103492086 PE=3 SV=1[more]
A0A0A0M1E90.090.70RNA helicase OS=Cucumis sativus OX=3659 GN=Csa_1G560830 PE=3 SV=1[more]
A0A6J1DUC80.088.83RNA helicase OS=Momordica charantia OX=3673 GN=LOC111024523 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G26742.11.5e-28270.98DEAD box RNA helicase (RH3) [more]
AT5G26742.27.5e-28271.01DEAD box RNA helicase (RH3) [more]
AT5G26742.31.1e-25977.11DEAD box RNA helicase (RH3) [more]
AT3G22330.18.4e-13263.00putative mitochondrial RNA helicase 2 [more]
AT3G22310.17.3e-12853.10putative mitochondrial RNA helicase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 737..753
e-value: 5.5E-5
score: 32.6
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 737..753
e-value: 2.2E-6
score: 27.5
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 738..753
score: 10.724101
IPR014001Helicase superfamily 1/2, ATP-binding domainSMARTSM00487ultradead3coord: 134..340
e-value: 5.1E-59
score: 212.1
IPR014001Helicase superfamily 1/2, ATP-binding domainPROSITEPS51192HELICASE_ATP_BIND_1coord: 146..323
score: 31.521574
IPR001650Helicase, C-terminalSMARTSM00490helicmild6coord: 377..457
e-value: 3.8E-31
score: 119.5
IPR001650Helicase, C-terminalPFAMPF00271Helicase_Ccoord: 351..457
e-value: 5.9E-31
score: 107.1
IPR001650Helicase, C-terminalPROSITEPS51194HELICASE_CTERcoord: 352..512
score: 22.813026
IPR011545DEAD/DEAH box helicase domainPFAMPF00270DEADcoord: 140..312
e-value: 1.0E-47
score: 162.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 338..529
e-value: 3.7E-51
score: 176.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 116..327
e-value: 1.7E-74
score: 251.7
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 193..478
IPR012562GUCTPFAMPF08152GUCTcoord: 544..644
e-value: 1.1E-17
score: 64.0
NoneNo IPR availableGENE3D4.10.60.10coord: 723..754
e-value: 5.4E-6
score: 27.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 638..754
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 657..695
NoneNo IPR availablePANTHERPTHR47959ATP-DEPENDENT RNA HELICASE RHLE-RELATEDcoord: 26..747
NoneNo IPR availablePANTHERPTHR47959:SF12SUBFAMILY NOT NAMEDcoord: 26..747
NoneNo IPR availableCDDcd00268DEADccoord: 126..324
e-value: 1.02525E-89
score: 277.786
NoneNo IPR availableCDDcd18787SF2_C_DEADcoord: 337..466
e-value: 6.91829E-54
score: 180.011
NoneNo IPR availableCDDcd12938GUCT_Heracoord: 551..631
e-value: 3.93104E-21
score: 85.8127
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 718..753

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG04g00220.1Cp4.1LG04g00220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000373 Group II intron splicing
biological_process GO:0006364 rRNA processing
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003724 RNA helicase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0004386 helicase activity
molecular_function GO:0003676 nucleic acid binding