Cp4.1LG03g17300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g17300
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG03: 12742450 .. 12743349 (-)
RNA-Seq Expression	Cp4.1LG03g17300
Synteny	Cp4.1LG03g17300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGTGATTCATAGCCGTTGGATCCTTATTGTTCTTGTTGTTCTTAATTTTCCGGCCGCCATTTTTAGTTATTCCGAAAAAGAATTAAAAAATTGGTGTAGCCAAACACCATATCCAGCTCCATGCGAGCAATTTATCAAAGCCAAAGCCACCGCCAAACATTCCCCAATCGCCACTAAATCCCATTTCCTTAAACTCTTGGTCGAAACGGCACTCGACGGCGCCGTTTCGGCCCAAAAAACCGCGCTGTCTCTCGGCCCAAAATGCCACAATGGCCGAGAAAAATCAGCATTGACCGACTGCGTTGACCTCTACAATCAAATCATTTCCCGTCTCAACCGCTCCGCCGACCGCTGCTCCTCCGTCGACGCCCAGACTTGGCTCAGCGCCTCCCTCACCGCCCTCGACACCTGCCGGAACAGCCTCCAGGAGCTCGGGGTTTCGGCCTTCGGCTACCCATTGACGAGCAATAATGTTTCCAAGCTCATTAGCGATGGGTTGTCTGTGAATAAGCCAACGTCACCCCAGGGGTTTGAGCCAACTATGACGGAGGGGTTTCCGACGTGGGTTAGGCCCGGTGACCGGAAACTGCTGCAGTCGGGGTCTCCGAGTGCGGACGTGGTGGTGGCGAAGGACGGGTCTGGGAATTTTAAGACGGTGAAGGAGGCGGTCGCCGCTGCGAAAGGCGGCGGGCGATTTGTAATATATGTGAAGGCGGGGGTTTATAGTGAAAACCTTGAGATTAATGCTAAGAATCTTATGTTGATCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGTAGAAGTGTCGGCGGCGGTTTCACCACGTTTAGATCCGCCACCGTTGGTATGTCTCTATCTCTCCTTATAAGCTTTTCTAAAATCTTTTTATAA

mRNA sequence

ATGGCAGTGATTCATAGCCGTTGGATCCTTATTGTTCTTGTTGTTCTTAATTTTCCGGCCGCCATTTTTAGTTATTCCGAAAAAGAATTAAAAAATTGGTGTAGCCAAACACCATATCCAGCTCCATGCGAGCAATTTATCAAAGCCAAAGCCACCGCCAAACATTCCCCAATCGCCACTAAATCCCATTTCCTTAAACTCTTGGTCGAAACGGCACTCGACGGCGCCGTTTCGGCCCAAAAAACCGCGCTGTCTCTCGGCCCAAAATGCCACAATGGCCGAGAAAAATCAGCATTGACCGACTGCGTTGACCTCTACAATCAAATCATTTCCCGTCTCAACCGCTCCGCCGACCGCTGCTCCTCCGTCGACGCCCAGACTTGGCTCAGCGCCTCCCTCACCGCCCTCGACACCTGCCGGAACAGCCTCCAGGAGCTCGGGGTTTCGGCCTTCGGCTACCCATTGACGAGCAATAATGTTTCCAAGCTCATTAGCGATGGGTTGTCTGTGAATAAGCCAACGTCACCCCAGGGGTTTGAGCCAACTATGACGGAGGGGTTTCCGACGTGGGTTAGGCCCGGTGACCGGAAACTGCTGCAGTCGGGGTCTCCGAGTGCGGACGTGGTGGTGGCGAAGGACGGGTCTGGGAATTTTAAGACGGTGAAGGAGGCGGTCGCCGCTGCGAAAGGCGGCGGGCGATTTGTAATATATGTGAAGGCGGGGGTTTATAGTGAAAACCTTGAGATTAATGCTAAGAATCTTATGTTGATCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGTAGAAGTGTCGGCGGCGGTTTCACCACGTTTAGATCCGCCACCGTTGGTATGTCTCTATCTCTCCTTATAAGCTTTTCTAAAATCTTTTTATAA

Coding sequence (CDS)

ATGGCAGTGATTCATAGCCGTTGGATCCTTATTGTTCTTGTTGTTCTTAATTTTCCGGCCGCCATTTTTAGTTATTCCGAAAAAGAATTAAAAAATTGGTGTAGCCAAACACCATATCCAGCTCCATGCGAGCAATTTATCAAAGCCAAAGCCACCGCCAAACATTCCCCAATCGCCACTAAATCCCATTTCCTTAAACTCTTGGTCGAAACGGCACTCGACGGCGCCGTTTCGGCCCAAAAAACCGCGCTGTCTCTCGGCCCAAAATGCCACAATGGCCGAGAAAAATCAGCATTGACCGACTGCGTTGACCTCTACAATCAAATCATTTCCCGTCTCAACCGCTCCGCCGACCGCTGCTCCTCCGTCGACGCCCAGACTTGGCTCAGCGCCTCCCTCACCGCCCTCGACACCTGCCGGAACAGCCTCCAGGAGCTCGGGGTTTCGGCCTTCGGCTACCCATTGACGAGCAATAATGTTTCCAAGCTCATTAGCGATGGGTTGTCTGTGAATAAGCCAACGTCACCCCAGGGGTTTGAGCCAACTATGACGGAGGGGTTTCCGACGTGGGTTAGGCCCGGTGACCGGAAACTGCTGCAGTCGGGGTCTCCGAGTGCGGACGTGGTGGTGGCGAAGGACGGGTCTGGGAATTTTAAGACGGTGAAGGAGGCGGTCGCCGCTGCGAAAGGCGGCGGGCGATTTGTAATATATGTGAAGGCGGGGGTTTATAGTGAAAACCTTGAGATTAATGCTAAGAATCTTATGTTGATCGGCGACGGAATCGGAAAAACTGTCATCACCGGAAGTAGAAGTGTCGGCGGCGGTTTCACCACGTTTAGATCCGCCACCGTTGGTATGTCTCTATCTCTCCTTATAAGCTTTTCTAAAATCTTTTTATAA

Protein sequence

MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGMSLSLLISFSKIFL

Homology

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BLAST of Cp4.1LG03g17300 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 296.6 bits (758), Expect = 3.2e-79
Identity = 153/279 (54.84%), Postives = 200/279 (71.68%), Query Frame = 0


Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I  V++ +   + +  Y++K++K WCSQTP P PCE F+     + + PI ++S FLK+ 
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL--THNSNNEPIKSESEFLKIS 68

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDA 128
           ++  LD A+ A+  A +LGPKC + REK+A  DC+ LY+  +S++N + D   +CS +DA
Sbjct: 69  MKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDA 128

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT LDTCR    ELGV+    PL SNNVS L+ + L++NK   P  + P   +
Sbjct: 129 QTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINK--VPFNYTPPEKD 188

Query: 189 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 248
           GFP+WV+PGDRKLLQS +P  + VVAKDGSGNFKT+KEA+ AA G GRFVIYVK GVYSE
Sbjct: 189 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYSE 248

Query: 249 NLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV 285
           NLEI  KN+ML GDGIGKT+ITGS+SVGGG TTF SATV
Sbjct: 249 NLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-73
Identity = 149/283 (52.65%), Postives = 201/283 (71.02%), Query Frame = 0


Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I + LV+ +     F YS +E+K+WC +TP P PCE F+  K     + I   + F K+ 
Sbjct: 7   ITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEYFLTQKTDV--TSIKQDTDFYKIS 66

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNR---SADRCSSVDA 128
           ++ AL+ A +AQ    +LG KC N REK+A  DC +LY   + +LN+   S+  C+ VD 
Sbjct: 67  LQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDK 126

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT L+TCR SL++LGV  +  PL SNNV+KLIS+ LS+NK   P   EP+  +
Sbjct: 127 QTWLSTALTNLETCRASLEDLGVPEYVLPLLSNNVTKLISNTLSLNK--VPYN-EPSYKD 186

Query: 189 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAK--GGGRFVIYVKAGVY 248
           GFPTWV+PGDRKLLQ+ +P A++VVA+DGSGN KT++EAVAAA   GG R+VIY+KAG Y
Sbjct: 187 GFPTWVKPGDRKLLQT-TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTY 246

Query: 249 SENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           +EN+E+  KN+M +GDGIGKT+ITGS+SVGGG TTF+SATV +
Sbjct: 247 NENIEVKLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAV 283

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 3.3e-52
Identity = 128/297 (43.10%), Postives = 176/297 (59.26%), Query Frame = 0


Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQ-FIKAKATAKHSPIATKSHFLKL 68
           +LI+L+ L     +   +   +  WC +TPYP PC++ FIK       + I   S F  L
Sbjct: 17  LLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQI---SEFRVL 76

Query: 69  LVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD--------R 128
           LVE A+D AVSA     +    C + ++++ L DC++LY   + +LNR+          R
Sbjct: 77  LVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRR 136

Query: 129 CSSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQG 188
           C+  DAQTWLS +LT  +TCR    +L VS F  P+ SN  +S LIS+ L+VN      G
Sbjct: 137 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 196

Query: 189 FEPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----KGG 248
              + T   +GFPTWV   +R+LLQ  S  A++VVAKDGSG+FKTV+ A+  A       
Sbjct: 197 KNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTS 256

Query: 249 GRFVIYVKAGVYSENLEI--NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           GRFVIYVK G+Y ENL +  N  N+ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 257 GRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.0e-49
Identity = 119/296 (40.20%), Postives = 175/296 (59.12%), Query Frame = 0


Query: 11  IVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVE 70
           ++L+VL     + + +++   +WC +TPYP PC+ + K        P    S F  +LVE
Sbjct: 15  LILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFK-NHNGFQQP-TQLSEFRVMLVE 74

Query: 71  TALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNR----------SADRC 130
            A+D A+SA+    + G  C + ++++ L DC+DLY   I +LNR          +A  C
Sbjct: 75  AAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSC 134

Query: 131 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGF 190
           +  DAQTWLS +LT  +TCR    +L V+ F  P+ SN  +S LIS+ L+VN      G 
Sbjct: 135 TDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTAGN 194

Query: 191 EPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----KGGG 250
           +   T   +GFPTW+   D++LL+  +  A++VVAKDGSG+F TV+ A+  A       G
Sbjct: 195 KGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRKVTSG 254

Query: 251 RFVIYVKAGVYSENLEINAKN--LMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           RFVIYVK G+Y EN+ +   N  +ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 255 RFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGI 306

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 6.3e-43
Identity = 112/291 (38.49%), Postives = 169/291 (58.08%), Query Frame = 0


Query: 33  WCSQTPYPAPCEQFIK--AKATAKHSPIATKSHFLKLLVETALDGAVSAQKTALSLGPKC 92
           WC++TP+   C  + +  ++      P   +S FL++LV+ ALD AV      +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 93  HNGREKSALTDCVDLYNQIISRLNR---------SAD-RCSSVDAQTWLSASLTALDTCR 152
            N + K+A +DCV+L+   +++LNR         S+D +C+  DAQTWLS + T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 153 NSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGFEPTMT---EGFPTWVRPGDR 212
           +  ++L VS F  P+ SN N+S LI + L+VN     Q    T     E FP+WV   +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 213 KLLQSGS---PSADVVVAKDGSGNFKTVKEAV---AAAKGGGRFVIYVKAGVYSENLEI- 272
           +LL S S    S  +VVA+D SG+F++++ A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 273 -NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGMSLSLLISFSKIFL 300
            +  N+ML+GDG  KT+IT  RSV  G+TT+ SAT G      ++    F+
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFI 377

[Close]

BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: XP_023527134.1 (pectinesterase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 559 bits (1441), Expect = 5.58e-197
Identity = 284/286 (99.30%), Postives = 285/286 (99.65%), Query Frame = 0


Query: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60
           MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT
Sbjct: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60

Query: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120
           KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC
Sbjct: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120

Query: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFE 180
           SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFE
Sbjct: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFE 180

Query: 181 PTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240
           PTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA
Sbjct: 181 PTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240

Query: 241 GVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           GVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV +
Sbjct: 241 GVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVAV 286

[Close]

BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: XP_022924664.1 (pectinesterase 2-like [Cucurbita moschata] >KAG6582550.1 Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018932.1 Pectinesterase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 554 bits (1428), Expect = 5.29e-195
Identity = 281/286 (98.25%), Postives = 283/286 (98.95%), Query Frame = 0


Query: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60
           MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT
Sbjct: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60

Query: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120
           KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC
Sbjct: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120

Query: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFE 180
           SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKP SPQGFE
Sbjct: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPASPQGFE 180

Query: 181 PTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240
           PTM EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA
Sbjct: 181 PTMAEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240

Query: 241 GVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           GVYSENLEINAKN+MLIGDGIGKTVITGSRSVGGGFTTFRSATV +
Sbjct: 241 GVYSENLEINAKNVMLIGDGIGKTVITGSRSVGGGFTTFRSATVAV 286

[Close]

BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: XP_022980272.1 (pectinesterase 2-like [Cucurbita maxima])

HSP 1 Score: 551 bits (1420), Expect = 8.70e-194
Identity = 279/286 (97.55%), Postives = 281/286 (98.25%), Query Frame = 0


Query: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60
           MAVIHSRWILIVLVVLNFP AIF YSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT
Sbjct: 1   MAVIHSRWILIVLVVLNFPGAIFGYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60

Query: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120
           KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC
Sbjct: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120

Query: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFE 180
           SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKP SPQGFE
Sbjct: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPASPQGFE 180

Query: 181 PTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240
           PTM EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA
Sbjct: 181 PTMAEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240

Query: 241 GVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           GVYSENLEINAKN+MLIGDGIGKTVITGSRSVGGGFTTFRSATV +
Sbjct: 241 GVYSENLEINAKNVMLIGDGIGKTVITGSRSVGGGFTTFRSATVAV 286

[Close]

BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: XP_038877776.1 (pectinesterase 2 [Benincasa hispida])

HSP 1 Score: 452 bits (1162), Expect = 1.30e-154
Identity = 223/281 (79.36%), Postives = 256/281 (91.10%), Query Frame = 0


Query: 7   RWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLK 66
           R +++V++VL FP  I+ YSE E+KNWCSQTPYPAPCE+F+K KAT K SPIATKSHF +
Sbjct: 6   RVLVVVVIVLIFPLDIYCYSEIEIKNWCSQTPYPAPCEEFLKTKATTKRSPIATKSHFFE 65

Query: 67  LLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVDAQ 126
           +LVETAL+ AVSA++ ALSLGPKC N +E++A TDCVDLY+QII+RLNR+ DRC+ VDAQ
Sbjct: 66  ILVETALERAVSARQNALSLGPKCRNSKERTAWTDCVDLYDQIITRLNRTVDRCTPVDAQ 125

Query: 127 TWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEP-TMTE 186
           TWLSA+LTAL+TCR  L+ELGVSAFGYPLT+NNVSKLISDGLSVNKP SP+GFEP TM +
Sbjct: 126 TWLSAALTALETCRTGLEELGVSAFGYPLTANNVSKLISDGLSVNKPASPEGFEPPTMED 185

Query: 187 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 246
           GFPTWVRPGDR+LLQSGSP A+VVVA+DGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE
Sbjct: 186 GFPTWVRPGDRRLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 245

Query: 247 NLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           N+EINAKN+MLIGDGIGKTVITGSRSVGGG TTFRSATV +
Sbjct: 246 NVEINAKNVMLIGDGIGKTVITGSRSVGGGSTTFRSATVAV 286

[Close]

BLAST of Cp4.1LG03g17300 vs. NCBI nr
Match: XP_008438193.1 (PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 439 bits (1128), Expect = 1.99e-149
Identity = 217/285 (76.14%), Postives = 251/285 (88.07%), Query Frame = 0


Query: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHS-PIATKSH 64
           HS   L+VLVVL FP  +  YSEKE+KNWCSQTPYPAPCE+F+K KA+ K S PI TKSH
Sbjct: 4   HSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKSH 63

Query: 65  FLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSV 124
           F ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY++II+RLNR++DRC+  
Sbjct: 64  FFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTPA 123

Query: 125 DAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPT- 184
           DAQTWLSA+LTAL+TCR    ELG+SAFGYPLT+NNVSKLISDGLSVNKP SP+G+EPT 
Sbjct: 124 DAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 183

Query: 185 -MTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAG 244
            M +GFPTWVRPGDRKLLQSGSP A+VVVA+DGSGNFKTVKEAVAAAKGGGRFVIYVK+G
Sbjct: 184 IMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKSG 243

Query: 245 VYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           VY+ENL+I AKN+M++GDGIGKT+ITGSRSVGGG TTFRSATV +
Sbjct: 244 VYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAV 288

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy TrEMBL
Match: A0A6J1E9U7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111432099 PE=3 SV=1)

HSP 1 Score: 554 bits (1428), Expect = 2.56e-195
Identity = 281/286 (98.25%), Postives = 283/286 (98.95%), Query Frame = 0


Query: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60
           MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT
Sbjct: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60

Query: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120
           KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC
Sbjct: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120

Query: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFE 180
           SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKP SPQGFE
Sbjct: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPASPQGFE 180

Query: 181 PTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240
           PTM EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA
Sbjct: 181 PTMAEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240

Query: 241 GVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           GVYSENLEINAKN+MLIGDGIGKTVITGSRSVGGGFTTFRSATV +
Sbjct: 241 GVYSENLEINAKNVMLIGDGIGKTVITGSRSVGGGFTTFRSATVAV 286

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy TrEMBL
Match: A0A6J1IQX9 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479692 PE=3 SV=1)

HSP 1 Score: 551 bits (1420), Expect = 4.21e-194
Identity = 279/286 (97.55%), Postives = 281/286 (98.25%), Query Frame = 0


Query: 1   MAVIHSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60
           MAVIHSRWILIVLVVLNFP AIF YSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT
Sbjct: 1   MAVIHSRWILIVLVVLNFPGAIFGYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIAT 60

Query: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120
           KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC
Sbjct: 61  KSHFLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRC 120

Query: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFE 180
           SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKP SPQGFE
Sbjct: 121 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPASPQGFE 180

Query: 181 PTMTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240
           PTM EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA
Sbjct: 181 PTMAEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKA 240

Query: 241 GVYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           GVYSENLEINAKN+MLIGDGIGKTVITGSRSVGGGFTTFRSATV +
Sbjct: 241 GVYSENLEINAKNVMLIGDGIGKTVITGSRSVGGGFTTFRSATVAV 286

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy TrEMBL
Match: A0A5A7U603 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G002830 PE=3 SV=1)

HSP 1 Score: 439 bits (1128), Expect = 9.61e-150
Identity = 217/285 (76.14%), Postives = 251/285 (88.07%), Query Frame = 0


Query: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHS-PIATKSH 64
           HS   L+VLVVL FP  +  YSEKE+KNWCSQTPYPAPCE+F+K KA+ K S PI TKSH
Sbjct: 4   HSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKSH 63

Query: 65  FLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSV 124
           F ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY++II+RLNR++DRC+  
Sbjct: 64  FFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTPA 123

Query: 125 DAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPT- 184
           DAQTWLSA+LTAL+TCR    ELG+SAFGYPLT+NNVSKLISDGLSVNKP SP+G+EPT 
Sbjct: 124 DAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 183

Query: 185 -MTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAG 244
            M +GFPTWVRPGDRKLLQSGSP A+VVVA+DGSGNFKTVKEAVAAAKGGGRFVIYVK+G
Sbjct: 184 IMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKSG 243

Query: 245 VYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           VY+ENL+I AKN+M++GDGIGKT+ITGSRSVGGG TTFRSATV +
Sbjct: 244 VYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAV 288

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy TrEMBL
Match: A0A1S3AVW4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1)

HSP 1 Score: 439 bits (1128), Expect = 9.61e-150
Identity = 217/285 (76.14%), Postives = 251/285 (88.07%), Query Frame = 0


Query: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHS-PIATKSH 64
           HS   L+VLVVL FP  +  YSEKE+KNWCSQTPYPAPCE+F+K KA+ K S PI TKSH
Sbjct: 4   HSIVFLVVLVVLIFPWDVLCYSEKEIKNWCSQTPYPAPCEEFLKTKASTKKSTPITTKSH 63

Query: 65  FLKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSV 124
           F ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY++II+RLNR++DRC+  
Sbjct: 64  FFEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDEIITRLNRTSDRCTPA 123

Query: 125 DAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPT- 184
           DAQTWLSA+LTAL+TCR    ELG+SAFGYPLT+NNVSKLISDGLSVNKP SP+G+EPT 
Sbjct: 124 DAQTWLSAALTALETCRTGFDELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTT 183

Query: 185 -MTEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAG 244
            M +GFPTWVRPGDRKLLQSGSP A+VVVA+DGSGNFKTVKEAVAAAKGGGRFVIYVK+G
Sbjct: 184 IMDDGFPTWVRPGDRKLLQSGSPKANVVVAQDGSGNFKTVKEAVAAAKGGGRFVIYVKSG 243

Query: 245 VYSENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           VY+ENL+I AKN+M++GDGIGKT+ITGSRSVGGG TTFRSATV +
Sbjct: 244 VYNENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAV 288

[Close]

BLAST of Cp4.1LG03g17300 vs. ExPASy TrEMBL
Match: A0A0A0L7N4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1)

HSP 1 Score: 437 bits (1123), Expect = 5.15e-149
Identity = 211/283 (74.56%), Postives = 250/283 (88.34%), Query Frame = 0


Query: 5   HSRWILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHF 64
           H+    +VL+VL FP+ I  YSEKE+KNWCSQTPYPAPCE+F+K KAT K +PI TKSHF
Sbjct: 4   HTIVFFVVLIVLIFPSDILCYSEKEIKNWCSQTPYPAPCEEFLKTKATTKKTPITTKSHF 63

Query: 65  LKLLVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSADRCSSVD 124
            ++LVETAL+ AVSA K ALSLGPKC N +EK+A TDCVDLY+QII+RLNR++ RCS  D
Sbjct: 64  FEILVETALERAVSAHKNALSLGPKCRNSKEKTAWTDCVDLYDQIITRLNRTSARCSPAD 123

Query: 125 AQTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPT-M 184
           AQTWLSA+LTAL+TCR   +ELG+SAFGYPLT+NNVSKLISDGLSVNKP SP+G+EPT M
Sbjct: 124 AQTWLSAALTALETCRTGFEELGLSAFGYPLTANNVSKLISDGLSVNKPASPEGYEPTTM 183

Query: 185 TEGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVY 244
           T+GFPTWV PG+RKLLQS SP ADVVVA+DGSGNFKTVK+A++AAKGGGRFVIY+K+GVY
Sbjct: 184 TDGFPTWVSPGNRKLLQSESPKADVVVAQDGSGNFKTVKDAISAAKGGGRFVIYIKSGVY 243

Query: 245 SENLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 286
           +ENL+I AKN+M++GDGIGKT+ITGSRSVGGG TTFRSATV +
Sbjct: 244 NENLDIKAKNVMMVGDGIGKTIITGSRSVGGGSTTFRSATVAV 286

[Close]

BLAST of Cp4.1LG03g17300 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 296.6 bits (758), Expect = 2.3e-80
Identity = 153/279 (54.84%), Postives = 200/279 (71.68%), Query Frame = 0


Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLL 68
           I  V++ +   + +  Y++K++K WCSQTP P PCE F+     + + PI ++S FLK+ 
Sbjct: 9   INFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFL--THNSNNEPIKSESEFLKIS 68

Query: 69  VETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD---RCSSVDA 128
           ++  LD A+ A+  A +LGPKC + REK+A  DC+ LY+  +S++N + D   +CS +DA
Sbjct: 69  MKLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDA 128

Query: 129 QTWLSASLTALDTCRNSLQELGVSAFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTE 188
           QTWLS +LT LDTCR    ELGV+    PL SNNVS L+ + L++NK   P  + P   +
Sbjct: 129 QTWLSTALTNLDTCRAGFLELGVTDIVLPLMSNNVSNLLCNTLAINK--VPFNYTPPEKD 188

Query: 189 GFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAAKGGGRFVIYVKAGVYSE 248
           GFP+WV+PGDRKLLQS +P  + VVAKDGSGNFKT+KEA+ AA G GRFVIYVK GVYSE
Sbjct: 189 GFPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAASGSGRFVIYVKQGVYSE 248

Query: 249 NLEINAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATV 285
           NLEI  KN+ML GDGIGKT+ITGS+SVGGG TTF SATV
Sbjct: 249 NLEIRKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATV 283

[Close]

BLAST of Cp4.1LG03g17300 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 206.8 bits (525), Expect = 2.4e-53
Identity = 128/297 (43.10%), Postives = 176/297 (59.26%), Query Frame = 0


Query: 9   ILIVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQ-FIKAKATAKHSPIATKSHFLKL 68
           +LI+L+ L     +   +   +  WC +TPYP PC++ FIK       + I   S F  L
Sbjct: 17  LLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQI---SEFRVL 76

Query: 69  LVETALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNRSAD--------R 128
           LVE A+D AVSA     +    C + ++++ L DC++LY   + +LNR+          R
Sbjct: 77  LVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRR 136

Query: 129 CSSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQG 188
           C+  DAQTWLS +LT  +TCR    +L VS F  P+ SN  +S LIS+ L+VN      G
Sbjct: 137 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 196

Query: 189 FEPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----KGG 248
              + T   +GFPTWV   +R+LLQ  S  A++VVAKDGSG+FKTV+ A+  A       
Sbjct: 197 KNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTS 256

Query: 249 GRFVIYVKAGVYSENLEI--NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           GRFVIYVK G+Y ENL +  N  N+ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 257 GRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGI 310

[Close]

BLAST of Cp4.1LG03g17300 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 197.6 bits (501), Expect = 1.4e-50
Identity = 119/296 (40.20%), Postives = 175/296 (59.12%), Query Frame = 0


Query: 11  IVLVVLNFPAAIFSYSEKELKNWCSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVE 70
           ++L+VL     + + +++   +WC +TPYP PC+ + K        P    S F  +LVE
Sbjct: 15  LILLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFK-NHNGFQQP-TQLSEFRVMLVE 74

Query: 71  TALDGAVSAQKTALSLGPKCHNGREKSALTDCVDLYNQIISRLNR----------SADRC 130
            A+D A+SA+    + G  C + ++++ L DC+DLY   I +LNR          +A  C
Sbjct: 75  AAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPKAGAAKSC 134

Query: 131 SSVDAQTWLSASLTALDTCRNSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGF 190
           +  DAQTWLS +LT  +TCR    +L V+ F  P+ SN  +S LIS+ L+VN      G 
Sbjct: 135 TDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNGALLTAGN 194

Query: 191 EPTMT---EGFPTWVRPGDRKLLQSGSPSADVVVAKDGSGNFKTVKEAVAAA----KGGG 250
           +   T   +GFPTW+   D++LL+  +  A++VVAKDGSG+F TV+ A+  A       G
Sbjct: 195 KGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAGRRKVTSG 254

Query: 251 RFVIYVKAGVYSENLEINAKN--LMLIGDGIGKTVITGSRSVGGGFTTFRSATVGM 287
           RFVIYVK G+Y EN+ +   N  +ML+GDG+  T+ITG RSV GG+TT+ SAT G+
Sbjct: 255 RFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSATAGI 306

[Close]

BLAST of Cp4.1LG03g17300 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 176.0 bits (445), Expect = 4.5e-44
Identity = 112/291 (38.49%), Postives = 169/291 (58.08%), Query Frame = 0


Query: 33  WCSQTPYPAPCEQFIK--AKATAKHSPIATKSHFLKLLVETALDGAVSAQKTALSLGPKC 92
           WC++TP+   C  + +  ++      P   +S FL++LV+ ALD AV      +  GP C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 93  HNGREKSALTDCVDLYNQIISRLNR---------SAD-RCSSVDAQTWLSASLTALDTCR 152
            N + K+A +DCV+L+   +++LNR         S+D +C+  DAQTWLS + T ++TCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 153 NSLQELGVSAFGYPLTSN-NVSKLISDGLSVNKPTSPQGFEPTMT---EGFPTWVRPGDR 212
           +  ++L VS F  P+ SN N+S LI + L+VN     Q    T     E FP+WV   +R
Sbjct: 207 SGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSWVSRHER 266

Query: 213 KLLQSGS---PSADVVVAKDGSGNFKTVKEAV---AAAKGGGRFVIYVKAGVYSENLEI- 272
           +LL S S    S  +VVA+D SG+F++++ A+   A  +   RFVIYVK GVY EN+++ 
Sbjct: 267 RLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYRENIDVG 326

Query: 273 -NAKNLMLIGDGIGKTVITGSRSVGGGFTTFRSATVGMSLSLLISFSKIFL 300
            +  N+ML+GDG  KT+IT  RSV  G+TT+ SAT G      ++    F+
Sbjct: 327 NDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFI 377

[Close]

BLAST of Cp4.1LG03g17300 vs. TAIR 10
Match: AT4G00190.1 (pectin methylesterase 38 )

HSP 1 Score: 165.2 bits (417), Expect = 7.9e-41
Identity = 107/260 (41.15%), Postives = 153/260 (58.85%), Query Frame = 0


Query: 34  CSQTPYPAPCEQFIKAKATAKHSPIATKSHFLKLLVETALDGAVSAQKTALSLGPKCHNG 93
           C +TP+P  C+  +      KH PI + + FL++ VE  LDGAV A+     L P+  + 
Sbjct: 8   CDETPHPGECKTLL-----IKHKPIRSTTQFLQVSVERTLDGAVKAKSDTYFLEPQFGS- 67

Query: 94  REKSALTDCVDLYNQIISRLNRSA----DRCSSVDAQTWLSASLTALDTCRNSLQELGVS 153
             K A  +C+DLY Q I RLN S     + CS  D Q WLS +LT LDTC+  + ELGVS
Sbjct: 68  --KQAWEECMDLYEQTIHRLNESVLCPKNVCSRSDVQAWLSTALTNLDTCQEEMSELGVS 127

Query: 154 AFGYPLTSNNVSKLISDGLSVNKPTSPQGFEPTMTEGFPTWVRPGDRKLLQSGSPSADVV 213
           +  + L S  +   + + L++NK     G E  +++     +  G++          DVV
Sbjct: 128 S--HSLESITID--VINTLAINKRMEQNGKEFGISKITMKTLSIGEK---------VDVV 187

Query: 214 VAKDGSGNFKTVKEAVAAA----KGGGRFVIYVKAGVYSE--NLEINAKNLMLIGDGIGK 273
           VA+DGSG++KT++EAV  A    KG  R+VI+VK GVY E  N+ I + N+M+ GDGIGK
Sbjct: 188 VAQDGSGDYKTIQEAVNGAGERLKGSPRYVIHVKQGVYEEYVNVGIKSNNIMITGDGIGK 246

Query: 274 TVITGSRSVGGGFTTFRSAT 284
           T+ITG +S G GF+T++SAT
Sbjct: 248 TIITGDKSKGRGFSTYKSAT 246

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g17300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22149	3.2e-79	54.84	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
O04887	1.2e-73	52.65	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9FHN4	3.3e-52	43.10	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	2.0e-49	40.20	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	6.3e-43	38.49	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Cp4.1LG03g17300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527134.1	5.58e-197	99.30	pectinesterase 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022924664.1	5.29e-195	98.25	pectinesterase 2-like [Cucurbita moschata] >KAG6582550.1 Pectinesterase 2, parti...	[more]
XP_022980272.1	8.70e-194	97.55	pectinesterase 2-like [Cucurbita maxima]	[more]
XP_038877776.1	1.30e-154	79.36	pectinesterase 2 [Benincasa hispida]	[more]
XP_008438193.1	1.99e-149	76.14	PREDICTED: pectinesterase 2 [Cucumis melo] >KAA0049055.1 pectinesterase 2 [Cucum...	[more]

BLAST of Cp4.1LG03g17300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E9U7	2.56e-195	98.25	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111432099 PE=3 SV=1	[more]
A0A6J1IQX9	4.21e-194	97.55	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479692 PE=3 SV=1	[more]
A0A5A7U603	9.61e-150	76.14	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00283...	[more]
A0A1S3AVW4	9.61e-150	76.14	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483370 PE=3 SV=1	[more]
A0A0A0L7N4	5.15e-149	74.56	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G126810 PE=3 SV=1	[more]

BLAST of Cp4.1LG03g17300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	2.3e-80	54.84	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	2.4e-53	43.10	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	1.4e-50	40.20	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	4.5e-44	38.49	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G00190.1	7.9e-41	41.15	pectin methylesterase 38	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 25..170 e-value: 5.9E-29 score: 112.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..159 e-value: 1.3E-22 score: 77.9
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 30..167 e-value: 5.7E-23 score: 81.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 202..292 e-value: 1.1E-24 score: 89.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 24..172 e-value: 5.6E-23 score: 83.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..171
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 207..287 e-value: 9.7E-25 score: 86.9
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 11..285
None	No IPR available	PANTHER	PTHR31707:SF300	PECTINESTERASE	coord: 11..285
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 33..172 e-value: 4.6918E-26 score: 98.6644
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 204..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g17300.1	Cp4.1LG03g17300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity