Cp4.1LG03g17170 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g17170 vs. ExPASy Swiss-Prot
Match: Q9LZX8 (Brefeldin A-inhibited guanine nucleotide-exchange protein 2 OS=Arabidopsis thaliana OX=3702 GN=BIG2 PE=2 SV=1)

HSP 1 Score: 2537.3 bits (6575), Expect = 0.0e+00
Identity = 1339/1814 (73.81%), Postives = 1510/1814 (83.24%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEA SRLS+V+TPALEKIVKNASWRKHSKLA+ECK+VIERL S  K S P   S+ T
Sbjct: 1    MASSEADSRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQK-SPPPSSSAAT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSE+E +VPGPLNDGG  E+SLA+SE I SPLINA  +G+ KI +PA+DCIQKLIA+GY+
Sbjct: 61   DSESESSVPGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYI 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGE+DPSGG E  LL KLI+SVCKCHDLGD+++EL VLKTLLSA+ SISLRIHG CLL +
Sbjct: 121  RGESDPSGGAESLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLV 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            V+TCYDIYL SKNV+NQTTAKASLIQ+LVIVFRRMEADSSTVP+QPIVVAELMEP+EK+D
Sbjct: 181  VRTCYDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVS---IGAHDGAFETTTVETTNPADLLDS 300
            ADG+MTQFVQGFITKI+QDIDGVLNP   G  S    G  DGA+ TTTVETTNP DLLDS
Sbjct: 241  ADGTMTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDS 300

Query: 301  TDKDMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKL 360
            TDKDMLDAKYWEISMYK+ALEGRKGEL DG+ ERDDDLE+QI NKLRRDA LVFRALCKL
Sbjct: 301  TDKDMLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKL 360

Query: 361  SMKTPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNR 420
            SMK PPKE+ ADPQ M+GKI+ALELLKILLENAG               +  T +    +
Sbjct: 361  SMKAPPKESSADPQSMRGKILALELLKILLENAG--------------AVFRTSE----K 420

Query: 421  FLGAIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLE 480
            F   IKQ+LCLSLLKNSASTLMIIFQLSCSIFISLV+RFRAGLKAEIGVFFPMIVLRV+E
Sbjct: 421  FSADIKQFLCLSLLKNSASTLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVE 480

Query: 481  NVAQPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPP 540
            NVAQPNFQQKMIVLRF++KLC+DSQILVDIF+NYDCDVNSSNIFERMVNGLLKTAQGVPP
Sbjct: 481  NVAQPNFQQKMIVLRFLDKLCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPP 540

Query: 541  GVATTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNV 600
            G ATTL+PPQE  MKLEAMKCLVAILKSMGDWLN+QLR+P  +S  K +V E     G+ 
Sbjct: 541  GTATTLMPPQEAAMKLEAMKCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSP 600

Query: 601  PISNGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLIN 660
             ++NG  DE  +GSD++SE S   SD   IEQRRAYKLELQEGISLFNRKP KGIEFLIN
Sbjct: 601  QLANGNADESADGSDTYSESSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLIN 660

Query: 661  ANKVGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIR 720
            A KVG SPEEIA FLKDASGL+K+LIGDYLGEREDL+LKVMHAYVDSFDF+G+EFDEAIR
Sbjct: 661  AGKVGESPEEIAGFLKDASGLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIR 720

Query: 721  ALLKGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVK 780
              L+GFRLPGEAQKIDRIMEKFAERYCKCNPK F SAD+AYVLAYSVI+LNTDAHNPMVK
Sbjct: 721  TFLEGFRLPGEAQKIDRIMEKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVK 780

Query: 781  NKMSAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLG 840
            NKMSA+DFIRNNRGIDDGKDLP +Y++SLYERI+++EIKMK+D++  Q +Q  NSN++LG
Sbjct: 781  NKMSADDFIRNNRGIDDGKDLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLG 840

Query: 841  FDSILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCW 900
             D ILNIVIRK+W D   ETSDDL+KHMQEQFKE+ARKSES YYAATDVVIL+FMIE CW
Sbjct: 841  LDGILNIVIRKQWGDSYAETSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACW 900

Query: 901  APMLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA------------------------- 960
            APMLAAFSVPLD+SDD ++I +CLEGF +AIH T+                         
Sbjct: 901  APMLAAFSVPLDQSDDLIVINICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPA 960

Query: 961  -----------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQN 1020
                       AI+++ADEEGN+LQ+AWEHILTCVSRFE LHLLGEGAPPDATFFA  QN
Sbjct: 961  DIKQRNIEAIKAILRLADEEGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQN 1020

Query: 1021 ESEKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNAS-GVTSEQMNNLVSN 1080
            ESEKSKQ K  +LP+LK+KG G+ QYAA  V+RGSYDS  + G  S  V  EQM+++VSN
Sbjct: 1021 ESEKSKQPKQYILPVLKRKGPGKSQYAATGVLRGSYDSMSLGGKGSKNVRQEQMSSIVSN 1080

Query: 1081 LNMLEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIA 1140
            LN+LEQVG  EMN++F++SQKLNSEAI+DFVKALCKVSM+ELRS S+PRVFSLTKIVEIA
Sbjct: 1081 LNLLEQVG--EMNQVFSQSQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIA 1140

Query: 1141 HYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQ 1200
            HYNMNRIRLVWSSIW VLS FFVTIGCSENLSIAIFAMDSLRQLSMKFL+REELANYNFQ
Sbjct: 1141 HYNMNRIRLVWSSIWQVLSGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQ 1200

Query: 1201 NEFMKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNI 1260
            NEFM PFVIVMR+S+ VEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAA DDHKNI
Sbjct: 1201 NEFMTPFVIVMRRSNDVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNI 1260

Query: 1261 VLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATK 1320
            V L+FEIIEKIIR+YFPYITETETTTFTDCVNCL+AFTNNRF+KDISL++IAFLR+CATK
Sbjct: 1261 VFLSFEIIEKIIREYFPYITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATK 1320

Query: 1321 LAEGDLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSF 1380
            LAEGDL S S NK K  SGKI   S    K GK +      +NHLYFWFPLL+GLSELSF
Sbjct: 1321 LAEGDLNSPSTNKYKGTSGKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSF 1380

Query: 1381 DPRPEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPS--STSSEQ 1440
            DPRPEIRKSALQ++FDTLR HGH FSLPLWE+VFESVLFPIFDYVRH+IDPS    S++Q
Sbjct: 1381 DPRPEIRKSALQIMFDTLRNHGHLFSLPLWEKVFESVLFPIFDYVRHSIDPSGEDESADQ 1440

Query: 1441 GAD-SENGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSL 1500
            G+   E  ELD DAWLYETCTLALQLVVDLFVKFY+TVNPLL+KVL LLVSFIKRPHQSL
Sbjct: 1441 GSSGGEVDELDHDAWLYETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSL 1500

Query: 1501 AGIGIAAFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELN 1560
            AGIGIAAFVRLMS+A  LFSEEKW EVV AL EA   T PDF++ ++ +   RS R  LN
Sbjct: 1501 AGIGIAAFVRLMSDADGLFSEEKWLEVVSALKEAAKTTCPDFSYFLSEEYVARSQRSALN 1560

Query: 1561 -GESNAETNGSELPDDDTESLRT-QHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLS 1620
               SNAE+      D + ES RT  H+Y ++SDAKCRAAVQLLLIQAVME YNMYR  LS
Sbjct: 1561 IQNSNAESAAPTATDGNEESQRTATHLYAAISDAKCRAAVQLLLIQAVMEIYNMYRPQLS 1620

Query: 1621 TKNMLILFDALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFV 1680
             KN L+L DALHGVA HAH IN+++ LRS+LQE   +TQMQDPPLLRLENESYQICLTF+
Sbjct: 1621 AKNTLVLVDALHGVALHAHGINSNTILRSRLQELGPMTQMQDPPLLRLENESYQICLTFL 1680

Query: 1681 QNLILD---KPHSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPL 1740
            QNL+ D   K    EE E+E  LV +C EVL FY+ET+     +++ +S  ++  W IPL
Sbjct: 1681 QNLVADKTKKEEEEEEEEIESLLVNICQEVLNFYIETSSSAKKLQSESSRASEYRWRIPL 1740

Query: 1741 GSGKQRELATRAPLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTE 1767
            GSGK+REL+ RAPLIVA LQA+C L+EASFEKNL   FPLL++LISCEHGSNEVQ AL +
Sbjct: 1741 GSGKRRELSARAPLIVATLQAMCTLDEASFEKNLKCLFPLLANLISCEHGSNEVQTALAD 1793

BLAST of Cp4.1LG03g17170 vs. ExPASy Swiss-Prot
Match: Q9LPC5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 3 OS=Arabidopsis thaliana OX=3702 GN=BIG3 PE=1 SV=1)

HSP 1 Score: 2424.8 bits (6283), Expect = 0.0e+00
Identity = 1280/1807 (70.84%), Postives = 1475/1807 (81.63%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MAS+E  SRL +V+ PAL+K++KNASWRKHSKLAHECKSVIERL  SP+ SSP       
Sbjct: 1    MASTEVDSRLGRVVIPALDKVIKNASWRKHSKLAHECKSVIERL-RSPENSSP-----VA 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSE+  ++PGPL+DGG  E+SLAESE ILSPLINASS+GVLKI DPAVDCIQKLIA+GY+
Sbjct: 61   DSESGSSIPGPLHDGGAAEYSLAESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYV 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADP+GG E  LL+KLIE++CKCH+L D+ LELLVLKTLL+AVTSISLRIHGD LLQI
Sbjct: 121  RGEADPTGGPEALLLSKLIETICKCHELDDEGLELLVLKTLLTAVTSISLRIHGDSLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            V+TCY IYL S+NV+NQ TAKASL+QM VIVFRRMEADSSTVP+QPIVVAELMEP++K++
Sbjct: 181  VRTCYGIYLGSRNVVNQATAKASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSE 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            +D S TQ VQGFITKI+QDIDGV N A   K + G HDGAFET+   T NP DLLDSTDK
Sbjct: 241  SDPSTTQSVQGFITKIMQDIDGVFNSAN-AKGTFGGHDGAFETSLPGTANPTDLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYK+ALEGRKGELADGE E+DDD E+QIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKSALEGRKGELADGEVEKDDDSEVQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKE   DP+LM+GKIVALELLKILLENAG               +  T     +RFLG
Sbjct: 361  TPPKE---DPELMRGKIVALELLKILLENAG--------------AVFRTS----DRFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSAS LMIIFQLSCSI +SLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASNLMIIFQLSCSILLSLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QP+FQQKMIVLRF++KLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPG  
Sbjct: 481  QPDFQQKMIVLRFLDKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGTV 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQE  MKLEAMKCLVA+L+SMGDW+N+QLR+PDP+S K +E+ + + E G+ P+ 
Sbjct: 541  TTLLPPQEAAMKLEAMKCLVAVLRSMGDWVNKQLRLPDPYSAKMLEIVDRNLEEGSHPVE 600

Query: 601  NGTTD-EHG--EGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLIN 660
            NG  D  HG  E SDS SE+S+  SD   IEQRRAYKLELQEGIS+FN+KPKKGIEFLI 
Sbjct: 601  NGKGDGGHGGFERSDSQSELSSGNSDALAIEQRRAYKLELQEGISIFNQKPKKGIEFLIK 660

Query: 661  ANKVGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIR 720
            ANKVG SPEEIAAFLKDASGL+K+LIGDYLGEREDLSLKVMHAYVDSF+FQG+EFDEAIR
Sbjct: 661  ANKVGDSPEEIAAFLKDASGLNKTLIGDYLGEREDLSLKVMHAYVDSFEFQGMEFDEAIR 720

Query: 721  ALLKGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVK 780
            A L+GFRLPGEAQKIDRIMEKFAER+CKCNPK F SADTAYVLAYSVILLNTDAHNPMVK
Sbjct: 721  AFLRGFRLPGEAQKIDRIMEKFAERFCKCNPKDFSSADTAYVLAYSVILLNTDAHNPMVK 780

Query: 781  NKMSAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLG 840
            +KM+A+ FIRNNRGIDDGKDLPEEYL++LYERISRNEIKMKDD + PQ +Q TNS++LLG
Sbjct: 781  SKMTADGFIRNNRGIDDGKDLPEEYLRALYERISRNEIKMKDDGLGPQQKQPTNSSRLLG 840

Query: 841  FDSILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCW 900
             D+ILNIV+ +R +D NMETSDDLI+HMQE+FKE+ARKSESVYYAA+DV+IL+FM+EVCW
Sbjct: 841  LDTILNIVVPRRGDDMNMETSDDLIRHMQERFKEKARKSESVYYAASDVIILRFMVEVCW 900

Query: 901  APMLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA------------------------- 960
            APMLAAFSVPLD+SDD VI  LCLEGF +AIHVT+                         
Sbjct: 901  APMLAAFSVPLDQSDDAVITTLCLEGFHHAIHVTSVMSLKTHRDAFVTSLAKFTSLHSPA 960

Query: 961  -----------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQN 1020
                       AIVK+A+EEGN+LQ+AWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQ 
Sbjct: 961  DIKQKNIEAIKAIVKLAEEEGNYLQDAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQT 1020

Query: 1021 ESEKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASG-VTSEQMNNLVSN 1080
            ES  S  +K   +P +K++  G++QYAA+A++RGSYD +G+ G AS  VTSEQMNNL+SN
Sbjct: 1021 ESGNSPLAKPNSVPAIKERAPGKLQYAASAMIRGSYDGSGVAGKASNTVTSEQMNNLISN 1080

Query: 1081 LNMLEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIA 1140
            LN+LEQVG  +M+RIFTRSQ+LNSEAI+DFVKALCKVSM+ELRS SDPRVFSLTKIVEIA
Sbjct: 1081 LNLLEQVG--DMSRIFTRSQRLNSEAIIDFVKALCKVSMDELRSPSDPRVFSLTKIVEIA 1140

Query: 1141 HYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQ 1200
            HYNMNRIRLVWSSIWHVLSDFFVTIGCS+NLSIAIFAMDSLRQLSMKFL+REELANYNFQ
Sbjct: 1141 HYNMNRIRLVWSSIWHVLSDFFVTIGCSDNLSIAIFAMDSLRQLSMKFLEREELANYNFQ 1200

Query: 1201 NEFMKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNI 1260
            NEFMKPFV+VMRKS AVEIRELIIRCVSQMVLSRV+NVKSGWKSMFM+FTTAA D HKNI
Sbjct: 1201 NEFMKPFVVVMRKSGAVEIRELIIRCVSQMVLSRVDNVKSGWKSMFMIFTTAAHDAHKNI 1260

Query: 1261 VLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATK 1320
            V L+FE++EKIIRDYFP+ITETETTTFTDCVNCL+AFTN +F KDISL AIAFL++CA K
Sbjct: 1261 VFLSFEMVEKIIRDYFPHITETETTTFTDCVNCLVAFTNCKFEKDISLQAIAFLQYCARK 1320

Query: 1321 LAEGDLGSSSRNKDKELSGKISSLSPLRAKDGKHDT-EMTDKDNHLYFWFPLLAGLSELS 1380
            LAEG +GSS R            LSP   K GK D+ +  + D HLY WFPLLAGLSELS
Sbjct: 1321 LAEGYVGSSLRRN--------PPLSPQGGKIGKQDSGKFLESDEHLYSWFPLLAGLSELS 1380

Query: 1381 FDPRPEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQG 1440
            FDPR EIRK AL+VLFDTLR HG HFSL LWERVFESVLF IFDYVR  +DPS   S   
Sbjct: 1381 FDPRAEIRKVALKVLFDTLRNHGDHFSLALWERVFESVLFRIFDYVRQDVDPSEDDSTD- 1440

Query: 1441 ADSENGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAG 1500
                NGE+DQ++WLYETC+LALQLVVDLFV FY TVNPLLKKVL L VS IKRPHQSLAG
Sbjct: 1441 QRGYNGEVDQESWLYETCSLALQLVVDLFVNFYKTVNPLLKKVLMLFVSLIKRPHQSLAG 1500

Query: 1501 IGIAAFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGE 1560
             GIAA VRLM + G  FS E+W EVV  + EA  AT PDF+++ + D       L  +  
Sbjct: 1501 AGIAALVRLMRDVGHQFSNEQWLEVVSCIKEAADATSPDFSYVTSED-------LMEDVS 1560

Query: 1561 SNAETNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNM 1620
            +  ETN +    +D    R + ++  ++DAK +A++Q+ +IQAV + Y+MYR  L+  +M
Sbjct: 1561 NEDETNDN---SNDALRRRNRQLHAVVTDAKSKASIQIFVIQAVTDIYDMYRMSLTANHM 1620

Query: 1621 LILFDALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLI 1680
            L+LFDA+HG+ S+AH IN    LRSKLQE  S  + Q+ PLLRLENES+Q C+TF+ NLI
Sbjct: 1621 LMLFDAMHGIGSNAHKINADLLLRSKLQELGSSLESQEAPLLRLENESFQTCMTFLDNLI 1680

Query: 1681 LDKPHSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRE 1740
             D+P  Y EAE+E +L+ LC EVL+FY         +  S S      W +P GSGK++E
Sbjct: 1681 SDQPVGYNEAEIESHLISLCREVLEFY---------INISCSKEQSSRWAVPSGSGKKKE 1740

Query: 1741 LATRAPLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVG 1767
            L  RAPL+VA +Q + N+ E+ F+KNL   FPL+++LISCEHGS EVQ+AL++ML+ S+G
Sbjct: 1741 LTARAPLVVAAIQTLGNMGESLFKKNLPELFPLIATLISCEHGSGEVQVALSDMLQTSMG 1749

BLAST of Cp4.1LG03g17170 vs. ExPASy Swiss-Prot
Match: F4JSZ5 (Brefeldin A-inhibited guanine nucleotide-exchange protein 1 OS=Arabidopsis thaliana OX=3702 GN=BIG1 PE=2 SV=1)

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 838/1807 (46.38%), Postives = 1150/1807 (63.64%), Query Frame = 0

Query: 1    MASSE---AASRLSQVITPALEKIVKNASWRKHSKLAHECKSV---IERLASSPKPSSPS 60
            M+SS+    A+R  +VI P+L+KI+KNA+WRKH+ L   CKSV   +E L+ SP PSSP 
Sbjct: 1    MSSSQNLGGATRCGRVIGPSLDKIIKNAAWRKHTFLVSACKSVLDKLEALSDSPDPSSPL 60

Query: 61   PPSSPTDSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKL 120
               + +D++A                       +L PL+ +  +G  K+ +PA+DC  KL
Sbjct: 61   FGLTTSDADA-----------------------VLQPLLLSLDTGYAKVIEPALDCSFKL 120

Query: 121  IAYGYLRGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHG 180
             +   LRGE   S      LL KLI ++CK   +G++++EL VL+ LL+AV S  + I G
Sbjct: 121  FSLSLLRGEVCSSS--PDSLLYKLIHAICKVCGIGEESIELAVLRVLLAAVRSPRILIRG 180

Query: 181  DCLLQIVKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELME 240
            DCLL +V+TCY++YL   N  NQ  AK+ L Q+++IVF R EA+S    ++ + V +L+ 
Sbjct: 181  DCLLHLVRTCYNVYLGGFNGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVNDLLA 240

Query: 241  PIEKTDADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADL 300
              +K   +G+     QGFI  +I                          T  E   P D 
Sbjct: 241  ITDKNVNEGNSVHICQGFINDVI--------------------------TAGEAAPPPD- 300

Query: 301  LDSTDKDMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRAL 360
                            ++ +   EG      +G            G+K+R D FL+F+ L
Sbjct: 301  ---------------FALVQPPEEGASSTEDEG-----------TGSKIREDGFLLFKNL 360

Query: 361  CKLSMKTPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCL 420
            CKLSMK   +E   D  L++GK ++LELLK++++N G     D                 
Sbjct: 361  CKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLSD----------------- 420

Query: 421  YNRFLGAIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLR 480
              RFL AIKQ LCLSLLKNSA ++M IFQL C+IF +L+ ++R+G+K+E+G+FFPM+VLR
Sbjct: 421  -ERFLNAIKQLLCLSLLKNSALSVMSIFQLQCAIFTTLLRKYRSGMKSEVGIFFPMLVLR 480

Query: 481  VLENVAQPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQG 540
            VLENV QP+F QKM VL  +E +C D  +++DIF+N+DCDV S NIFER+VNGLLKTA G
Sbjct: 481  VLENVLQPSFVQKMTVLSLLENICHDPNLIIDIFVNFDCDVESPNIFERIVNGLLKTALG 540

Query: 541  VPPGVATTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSES 600
             PPG +T L P Q++T + E++KCLV+I+K+MG W+++QL + D    K +E    ++  
Sbjct: 541  PPPGSSTILSPVQDITFRHESVKCLVSIIKAMGTWMDQQLSVGDSLLPKSLENEAPANNH 600

Query: 601  GNVPISNGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEF 660
             N    +GTT +H    D H +++ + SD +T+EQRRAYK+E Q+G++LFNRKP KGIEF
Sbjct: 601  SNSNEEDGTTIDH----DFHPDLNPESSDAATLEQRRAYKIERQKGVTLFNRKPSKGIEF 660

Query: 661  LINANKVGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDE 720
            LI++ KVG+SP+E+ +FL++ +GL+ ++IGDYLGERED  +KVMHAYVDSFDF+ + F E
Sbjct: 661  LISSKKVGNSPDEVVSFLRNTTGLNATMIGDYLGEREDFPMKVMHAYVDSFDFKEMNFGE 720

Query: 721  AIRALLKGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNP 780
            AIR  L+GFRLPGEAQKIDRIMEKFAER+CKCNP +F SADTAYVLAYSVI+LNTDAHN 
Sbjct: 721  AIRFFLRGFRLPGEAQKIDRIMEKFAERFCKCNPNSFSSADTAYVLAYSVIMLNTDAHNI 780

Query: 781  MVKNKMSAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNK 840
            MVK KM+  DFIRNNRGIDDGKDLPEEYL +LY+++  NEIKM  D  AP+ RQS   NK
Sbjct: 781  MVKEKMTKADFIRNNRGIDDGKDLPEEYLGALYDQVVINEIKMSSDSSAPESRQSNGLNK 840

Query: 841  LLGFDSILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIE 900
            LLG D ILN+V   + E++ +  +  LIK +QE+F+ ++ KSES Y+  TDV IL+FM+E
Sbjct: 841  LLGLDGILNLVYWTQTEEKAVGANGLLIKDIQEKFRSKSGKSESAYHVVTDVAILRFMVE 900

Query: 901  VCWAPMLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------- 960
            V W PMLAAFSV LD+SDD +    CL GF+YA+HVTA                      
Sbjct: 901  VSWGPMLAAFSVTLDQSDDRLAAVECLRGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLH 960

Query: 961  --------------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAF 1020
                          AI+ IA E+GN LQ+AWEHILTC+SR EHL LLGEGAP DA++FA 
Sbjct: 961  CAGDMKQKNVDAVKAIISIAIEDGNHLQDAWEHILTCLSRIEHLQLLGEGAPSDASYFA- 1020

Query: 1021 PQNESEKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASG-VTSEQMNNL 1080
                S ++++ K+   P LKKKG  +     A V  GSYDS+ I  N  G V  +Q+NN 
Sbjct: 1021 ----STETEEKKALGFPNLKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNF 1080

Query: 1081 VSNLNMLEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIV 1140
            ++NLN+L+Q+GS ++N ++  SQ+L +EAIV FVKALCKVSM EL+S +DPRVFSLTK+V
Sbjct: 1081 IANLNLLDQIGSFQLNNVYAHSQRLKTEAIVAFVKALCKVSMSELQSPTDPRVFSLTKLV 1140

Query: 1141 EIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANY 1200
            EIAHYNMNRIRLVWS IW +LSDFFV++G SENLS+AIF MDSLRQLSMKFL+REELANY
Sbjct: 1141 EIAHYNMNRIRLVWSRIWSILSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANY 1200

Query: 1201 NFQNEFMKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDH 1260
            NFQNEF++PFVIVM+KSS+ EIRELI+RC+SQMVLSRV+NVKSGWKS+F VFTTAA+D+ 
Sbjct: 1201 NFQNEFLRPFVIVMQKSSSAEIRELIVRCISQMVLSRVSNVKSGWKSVFKVFTTAAADER 1260

Query: 1261 KNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFC 1320
            KNIVLLAFE +EKI+R+YF YITETE TTFTDCV CLI FTN+ F  D+SLNAIAFLRFC
Sbjct: 1261 KNIVLLAFETMEKIVREYFSYITETEATTFTDCVRCLITFTNSTFTSDVSLNAIAFLRFC 1320

Query: 1321 ATKLAEGDLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSE 1380
            A KLA+G L  + + +        S  +P+            D D ++ +W PLL GLS+
Sbjct: 1321 ALKLADGGLVWNEKGRSS------SPSTPVTDDHSPSTQNFMDADENISYWVPLLTGLSK 1380

Query: 1381 LSFDPRPEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSE 1440
            L+ D R  IRKS+L+VLF+ L+ HGH FS   W  VF SV++PIF+ V    D  S    
Sbjct: 1381 LTSDSRSAIRKSSLEVLFNILKDHGHIFSRTFWIGVFSSVIYPIFNSVWGENDLLSKDEH 1440

Query: 1441 QGADSE-NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQS 1500
                S  +    + +W  ET  +A Q +VDLFV F++ +   L  V++LL   I+ P Q 
Sbjct: 1441 SSFPSTFSSHPSEVSWDAETSAMAAQYLVDLFVSFFTVIRSQLSSVVSLLAGLIRSPAQG 1500

Query: 1501 LAGIGIAAFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLEL 1560
                G+ A +RL    GD FSE +W+E+  A+ EA S TL  F   + T   I       
Sbjct: 1501 PTVAGVGALLRLADELGDRFSENEWKEIFLAVNEAASLTLSSFMKTLRTMDDIPDE---- 1560

Query: 1561 NGESNAETNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLST 1620
              ++ ++ + S   D D +SL+T    ++ +  K    VQL ++Q V + Y +++  L  
Sbjct: 1561 --DTLSDQDFSNEDDIDEDSLQTMSYVVART--KSHITVQLQVVQVVTDLYRIHQQSLLA 1620

Query: 1621 KNMLILFDALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQ 1680
             ++ ++ + L  ++SHAH +N+   L+ K++   SI ++ +PP+L  EN+++Q  L  +Q
Sbjct: 1621 SHVTVILEILSSISSHAHQLNSDLILQKKVRRACSILELSEPPMLHFENDTFQNYLDILQ 1680

Query: 1681 NLILDKPHSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQP-HWPIPLGSG 1740
             ++ + P    E  VE  L+ +C ++L+ Y++     L          QP +W +P+G+ 
Sbjct: 1681 AIVTNNPGVSLELNVESQLMTVCMQILKMYLKCT---LFQGDELEETRQPKNWILPMGAA 1685

Query: 1741 KQRELATRAPLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLR 1763
             + E A R+PL+VA+L+A+  L   SF++    FFPLL  L+  EH S++V   L+ +  
Sbjct: 1741 SKEEAAARSPLVVAVLKALRELKRDSFKRYAPNFFPLLVELVRSEHSSSQVPQVLSTVFH 1685

BLAST of Cp4.1LG03g17170 vs. ExPASy Swiss-Prot
Match: F4JN05 (Brefeldin A-inhibited guanine nucleotide-exchange protein 4 OS=Arabidopsis thaliana OX=3702 GN=BIG4 PE=3 SV=1)

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 819/1796 (45.60%), Postives = 1133/1796 (63.08%), Query Frame = 0

Query: 7    ASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPTDSEAEG 66
            A+R  ++I P+L+KI+KNA+WRKH+ L   CKSV+++L S   P     PSS     A  
Sbjct: 10   ATRCGRIIGPSLDKIIKNAAWRKHTYLVSSCKSVLDKLES--LPDDFHDPSSVVSGLA-- 69

Query: 67   AVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYLRGEADP 126
                            ++++S+L P + +  +   K+ +P++DC  KL +   LRGE   
Sbjct: 70   ---------------ASDADSVLQPFLLSLETAYSKVVEPSLDCAFKLFSLSILRGEIQS 129

Query: 127  SGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQIVKTCYD 186
            S   +  +L KL+ +V K   + ++ ++L VL+ LL+AV S  + I GDCLL +VKTCY+
Sbjct: 130  S--KQDSILFKLVNAVSKVGAIAEEPIQLAVLRVLLAAVRSPCILIRGDCLLHVVKTCYN 189

Query: 187  IYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTDADGSMT 246
            IYL   +   Q  AK+ L QM++++F R E DS  V V+ I V EL+   +K+  +GS  
Sbjct: 190  IYLGGLSGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNELLTFTDKSVNEGSSV 249

Query: 247  QFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDKDMLDAK 306
             F QGF+ +++    G   P  P  + I   +   ET TV T +                
Sbjct: 250  YFCQGFVNEVMAAGQGSPLP-PPDVIQILLQNP--ETETVMTPDSPSF------------ 309

Query: 307  YWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMKTPPKEA 366
                          +G +A+GEG    D E    +K+R+DAFL+F+ LCKLSM+   KE 
Sbjct: 310  --------------RGYVANGEG----DSETGDMSKVRQDAFLLFKNLCKLSMRFSSKEN 369

Query: 367  MADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLGAIKQYL 426
              D  +++GK ++LELLK++++N G             W+   +       F+ A+KQYL
Sbjct: 370  NDDQIMVRGKTLSLELLKVIIDNGGSV-----------WRTNES-------FINAVKQYL 429

Query: 427  CLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPNFQQ 486
            CLSLLKNSA ++M IFQL C+IF+SL+S+ R+ LKAEIG+FFPMIVLRVLENV QP++ Q
Sbjct: 430  CLSLLKNSAVSIMSIFQLQCAIFMSLLSKLRSVLKAEIGIFFPMIVLRVLENVLQPSYLQ 489

Query: 487  KMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVATTLLPP 546
            KM VL  ++K+  D Q++VDIF+NYDCDV SSNI ER+VNGLLKTA G P G +TTL P 
Sbjct: 490  KMTVLNLLDKMSQDPQLMVDIFVNYDCDVESSNILERIVNGLLKTALGPPTGSSTTLSPA 549

Query: 547  QELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEV-AENSSESGNVPISNGTTD 606
            Q+ T + +++KCLV + K+MG+W+++QL++ +    K  +V A   S +  +    GT  
Sbjct: 550  QDSTFRNDSVKCLVNLAKAMGNWMDQQLKVNETVWPKGSQVYASMDSNASQISELEGTIS 609

Query: 607  EHGEGSDSHSEVST-DISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANKVGSS 666
            +     DS  + S  +  D S +EQRRAYK+ELQ+GISLFNRKP KG+EFLI+  K+GSS
Sbjct: 610  D----CDSQPDTSNPEAYDASMLEQRRAYKIELQKGISLFNRKPSKGVEFLISTKKIGSS 669

Query: 667  PEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALLKGFR 726
            PEE+A+FL   +GL+ ++IGDYLGER++L LKVMHAYVDSF+F+  +F EAIR  L+GFR
Sbjct: 670  PEEVASFLMKTAGLNGTVIGDYLGERDELPLKVMHAYVDSFNFEKKDFVEAIRFFLRGFR 729

Query: 727  LPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKMSAED 786
            LPGEAQKIDRIMEKFAE Y KCNP +F SADTAYVLAYSVI+LNTDAHN MVK+KM+  D
Sbjct: 730  LPGEAQKIDRIMEKFAEHYWKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKDKMTKAD 789

Query: 787  FIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDSILNI 846
            F+RNNRGIDDGKDLPEEYL SLY+R+ + EI+M  D +APQ +Q    NKLLG D ILN+
Sbjct: 790  FVRNNRGIDDGKDLPEEYLGSLYDRVVKEEIRMNSDTLAPQNKQVNGLNKLLGLDGILNL 849

Query: 847  VIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPMLAAF 906
            V   + +++    +  LI+ +QEQF+ +  KSESVY+  TD+ IL+F++EV W PMLAAF
Sbjct: 850  VSWMQPDEKPHGANGRLIRDIQEQFQAKPEKSESVYHTVTDISILRFILEVSWGPMLAAF 909

Query: 907  SVPLDRSDDEVIIALCLEGFQYAIHVTA-------------------------------- 966
            SV +D+SDD +  +LCL+GF+YA+HVTA                                
Sbjct: 910  SVTIDQSDDRLATSLCLQGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAADMKQKNV 969

Query: 967  ----AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESEKSKQ 1026
                AI+ IA E+GN L  +WEHILTC+SR EHL LLGE +P +  +   P  ++E    
Sbjct: 970  DAVKAIITIAIEDGNHLHGSWEHILTCLSRIEHLQLLGEVSPSEKRY--VPTKKAEVD-D 1029

Query: 1027 SKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASG-VTSEQMNNLVSNLNMLEQV 1086
             K+   P LKK+G  +     A V  GSYDS  +  +    VT EQ+ + ++NLN+L+Q+
Sbjct: 1030 KKALGFPNLKKRGSFQNPSVMAVVRGGSYDSTSLVKSVPKLVTPEQIKSFIANLNLLDQI 1089

Query: 1087 GSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNMNRI 1146
            G+ E+N ++  SQ+LNSEAIV FVKALCKVSM EL+S +DPRVFSLTK+VE AHYNMNRI
Sbjct: 1090 GNFELNHVYANSQRLNSEAIVSFVKALCKVSMSELQSPTDPRVFSLTKLVETAHYNMNRI 1149

Query: 1147 RLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPF 1206
            RLVWS IW+VLSDFFV++G SENLS+AIF MDSLRQLSMKFL+REELANY+FQ+EF++PF
Sbjct: 1150 RLVWSRIWNVLSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYHFQHEFLRPF 1209

Query: 1207 VIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLAFEI 1266
            V+VM+KSS+ EIRELI+RCVSQMVLSRV+NVKSGWK++F VFTTAA D+ KNIVLLAFE 
Sbjct: 1210 VVVMQKSSSAEIRELIVRCVSQMVLSRVSNVKSGWKNVFTVFTTAALDERKNIVLLAFET 1269

Query: 1267 IEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEGDLG 1326
            IEKI+RD+F  I ETE T + DC+ CLI FTN++F  DI  N I FLRFCA KL EG L 
Sbjct: 1270 IEKIVRDHFHCIIETEITVYADCIRCLITFTNSKFEGDIGFNTIEFLRFCALKLEEGGLV 1329

Query: 1327 SSSRNKDKELSGKISSLSPLRAKDGKHDTE-MTDKDNHLYFWFPLLAGLSELSFDPRPEI 1386
             + + K+  +S           K+   DT+  TD D  + +W PLL GL +   DPRP I
Sbjct: 1330 LNEKLKNNTISA---------LKEDFSDTQSFTDLDEQVSYWIPLLTGLCKQVSDPRPAI 1389

Query: 1387 RKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSENGE 1446
            RK +++VLF  L  HGH F+ P W  +F S++ P+F+ +R   D     S     S + +
Sbjct: 1390 RKRSIEVLFHILMDHGHLFTRPFWTGIFSSIILPVFNNIRSKTDMLFEESVDSPSSASLD 1449

Query: 1447 LDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAAFV 1506
             ++  W  ET TLALQL+VDL VKF+ +V   L  V++++V FIK P Q   G GI+  +
Sbjct: 1450 TEETTWDVETSTLALQLLVDLLVKFFRSVRSQLPSVVSIIVGFIKSPFQGSTGSGISVLL 1509

Query: 1507 RLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAETNG 1566
             L        SE++W+E+  AL EA S T   F  ++ T   I      L+G+S    N 
Sbjct: 1510 HLADGLARSASEDEWREIFLALKEAASLTFAGFMKVLRTMDDIEDVE-TLSGQS---VNI 1569

Query: 1567 SELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFDAL 1626
             +L DD    +     Y+ +S  K    V   +++ V + Y   +  LS  ++ IL D  
Sbjct: 1570 GDLDDDSLHIMS----YV-VSRTKKHIDVLSQIVEVVSDLYRRNQFSLSASHVDILADIF 1629

Query: 1627 HGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPHSY 1686
              +ASHA  +NT + LR K +   S+  + +P LL  ENE+Y+  + F+Q+++   P+  
Sbjct: 1630 SCIASHAQQLNTDTVLRRKFKRACSVQNLTEPQLLNFENEAYKSYMMFLQDMVTCNPNVS 1689

Query: 1687 EEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATRAPL 1746
            +E ++E  LV  C ++++ Y++        +          W +P+ S +  E   R  L
Sbjct: 1690 KELDLESRLVTECAKIVKIYLKCTD---PQQQEQQQRKPVLWVLPMESDRVEEATARTSL 1705

Query: 1747 IVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPIL 1763
            +V+ L+A+C+L   S +K+++ FFPLL  L+  EH S +V   L+ +L++ +GPIL
Sbjct: 1750 LVSSLEALCSLEAESLKKHVSSFFPLLVDLVRTEHCSPQVPYVLSNVLKSCIGPIL 1705

BLAST of Cp4.1LG03g17170 vs. ExPASy Swiss-Prot
Match: F4IXW2 (Brefeldin A-inhibited guanine nucleotide-exchange protein 5 OS=Arabidopsis thaliana OX=3702 GN=BIG5 PE=1 SV=2)

HSP 1 Score: 839.3 bits (2167), Expect = 7.8e-242
Identity = 635/1832 (34.66%), Postives = 902/1832 (49.24%), Query Frame = 0

Query: 83   AESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYLRGEADPSGGVEGKLLAKLIESV 142
            AE E +L PL  A  +  LKI D A+DC+ KLIAY +L G+    GG        ++  V
Sbjct: 102  AEVELVLKPLRLAFETKNLKIFDAALDCLHKLIAYDHLEGDPGLDGGKNSAPFTDILNMV 161

Query: 143  CKCHD-LGDDALELLVLKTLLSAVTSISLRIHGDCLLQIVKTCYDIYLDSKNVINQTTAK 202
            C C D    D+  L VLK LL+AV S   ++HG+ LL +++ CY+I L+SK+ INQ T+K
Sbjct: 162  CSCVDNSSPDSTVLQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPINQATSK 221

Query: 203  ASLIQMLVIVFRRMEAD----SSTVPVQPIVVAELMEP--IEKTDADGSMTQFVQGFITK 262
            A L QM+ IVFRRME D    SSTV  +  V  +   P   E T AD +  +   G    
Sbjct: 222  AMLTQMISIVFRRMETDIVSASSTVSQEEHVSGDTSSPKNEEITAADENEKEMTLG---- 281

Query: 263  IIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDKDMLDAKYWEISMYKT 322
                                A   A +TT         L+   D   L+A          
Sbjct: 282  -------------------DALTQAKDTTLASVEELHTLVGGADIKGLEA---------- 341

Query: 323  ALEGRKGELADGEG-ERDDDLE-IQIGNKLRRDAFLVFRALCKLSMKTPPKEAMADPQLM 382
            AL+ +   L DG+  +R  +LE + IG   +RDA LVFR LCK+ MK       +D    
Sbjct: 342  ALD-KAVHLEDGKKIKRGIELESMSIG---QRDALLVFRTLCKMGMKED-----SDEVTT 401

Query: 383  KGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLGAIKQYLCLSLLKN 442
            K +I++LELL+ +LE        +F                   F+ ++K YL  +LL+ 
Sbjct: 402  KTRILSLELLQGMLEGVSHSFTKNF------------------HFIDSVKAYLSYALLRA 461

Query: 443  SASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPNFQQKMIVLRF 502
            S S   +IFQ +  IF  L+ RFR  LK EIG+FFP+IVLR L+N   PN  QKM VLR 
Sbjct: 462  SVSQSSVIFQYASGIFSVLLLRFRDSLKGEIGIFFPIIVLRSLDNSECPN-DQKMGVLRM 521

Query: 503  VEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVATTLLPPQELTMKL 562
            +EK+C D Q+LVD+++NYDCD+ + N+FERMV  L K AQG         +  Q  ++K 
Sbjct: 522  LEKVCKDPQMLVDVYVNYDCDLEAPNLFERMVTTLSKIAQGSQSADPNPAMASQTASVKG 581

Query: 563  EAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPISNGTTDEHGEGSDS 622
             +++CLV +LKS+ DW             K    AENS+ + N        D    G   
Sbjct: 582  SSLQCLVNVLKSLVDW------------EKIRREAENSTRNAN-------EDSASTGEPI 641

Query: 623  HSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANKVGSSPEEIAAFLK 682
             ++   D+   S  E+ +A+K  ++  IS FNR   KG+E+LI    V  +P  +A FL+
Sbjct: 642  ETKSREDVP--SNFEKAKAHKSTMEAAISEFNRNSVKGVEYLIANKLVERNPASVAQFLR 701

Query: 683  DASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALLKGFRLPGEAQKID 742
              S L K +IGDYLG+ E+  L VMHAYVDS  F  ++F  AIR  LKGFRLPGEAQKID
Sbjct: 702  STSSLSKVMIGDYLGQHEEFPLAVMHAYVDSMKFSEMKFHSAIREFLKGFRLPGEAQKID 761

Query: 743  RIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKMSAEDFIRNNRGID 802
            RIMEKFAERYC  NP  F +ADTAYVLAY+VI+LNTDAHNPMV  KMS  DF R N   D
Sbjct: 762  RIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNATND 821

Query: 803  DGKDLPEEYLKSLYERISRNEIKMKDDEMAPQL-RQSTNSNKLLGFDSILNIVIRKRWE- 862
                 P E L+ +Y+ I + EIK+KDD+   +L  Q     +  G  SILN+ + KR   
Sbjct: 822  PEDCAPTELLEEIYDSIVQEEIKLKDDDTMKKLSSQRPGGEERGGLVSILNLGLPKRISA 881

Query: 863  -DQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPMLAAFSVPLDR 922
             D   ET +D+++  QE F++   K   V++    V I++ M+E    P+LAAFSV ++ 
Sbjct: 882  ADAKSET-EDIVRKTQEIFRKHGVK-RGVFHTVEQVDIIRPMVEAVGWPLLAAFSVTMEV 941

Query: 923  SDDEVIIALCLEGFQYAIHVT------------------------------------AAI 982
             D++  I LC+EGF+  IH+                                       +
Sbjct: 942  GDNKPRILLCMEGFKAGIHIAYVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRIL 1001

Query: 983  VKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESEKSKQSKSTML 1042
            + + D E + LQ+ W  +L CVSR E                                  
Sbjct: 1002 LGLCDSEPDTLQDTWNAVLECVSRLEF--------------------------------- 1061

Query: 1043 PLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNMLEQVGSSEMNR 1102
             ++   G+      AA VM GS        +  GV           +  L+++      +
Sbjct: 1062 -IISTPGI------AATVMHGSNQI-----SRDGV-----------VQSLKELAGRPAEQ 1121

Query: 1103 IFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNMNRIRLVWSSI 1162
            +F  S KL SE++V+F  ALC VS EEL+  S  RVFSL K+VEI++YN+ RIR+VW+ I
Sbjct: 1122 VFVNSVKLPSESVVEFFTALCGVSAEELKQ-SPARVFSLQKLVEISYYNIARIRMVWARI 1181

Query: 1163 WHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPFVIVMRKS 1222
            W VL++ FV+ G   +  IA++A+DSLRQL MK+L+R EL N+ FQN+ +KPFVI+MR +
Sbjct: 1182 WSVLAEHFVSAGSHHDEKIAMYAIDSLRQLGMKYLERAELTNFTFQNDILKPFVIIMRNT 1241

Query: 1223 SAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLAFEIIEKIIRD 1282
             +  IR LI+ C+ QM+ S+V ++KSGW+S+FM+FT AA D+ ++IV  +FE +E++I +
Sbjct: 1242 QSQTIRSLIVDCIVQMIKSKVGSIKSGWRSVFMIFTAAADDEVESIVEKSFENVEQVILE 1301

Query: 1283 YFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEGDLGSSSRNKD 1342
            +F  +       F DCVNCLI F NN+ +  ISL AIA LR C  +LAEG +        
Sbjct: 1302 HFDQVI---GDCFMDCVNCLIRFANNKASDRISLKAIALLRICEDRLAEGLIPG------ 1361

Query: 1343 KELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRPEIRKSALQVL 1402
                        L+  DG  D E  D   H  +WFP+LAGLS+L+ D RPE+R  AL+VL
Sbjct: 1362 ----------GVLKPVDGNED-ETFDVTEH--YWFPMLAGLSDLTSDYRPEVRNCALEVL 1421

Query: 1403 FDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSENGELDQDAWLY 1462
            FD L + G+ FS P WE +F  +LFPIFD+V HA   S  SS             D    
Sbjct: 1422 FDLLNERGNKFSTPFWESIFHRILFPIFDHVSHAGKESLISS------------GDVKFR 1481

Query: 1463 ETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAAFVRLMSNAGD 1522
            ET   +LQL+ +LF  FY  V  +L  +L+LL+   K+  Q++  I + A V L+   G 
Sbjct: 1482 ETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKKSDQTVVSISLGALVHLIEVGGH 1541

Query: 1523 LFSEEKWQEVVFALGEATSATLP------------------------------------- 1582
             FSE  W  ++ ++ +A+  T P                                     
Sbjct: 1542 QFSEGDWDMLLKSIRDASYTTQPLELLNALSFDNPKKNLVLAGDIEADASDSPRVDRNPD 1601

Query: 1583 ----DFTFLVNTDSTIRSHRLELNGESNAETNGSE------------------------- 1642
                +          I +H   L      + +GSE                         
Sbjct: 1602 DIKDNGKVSAQASPRIGTHGTSLESGIPPKADGSEGRPSSSGRAQKDVDDVNLQRSQTFG 1661

Query: 1643 ------------------------LP------DDDTESLRTQHVYISMSDAKCRAAVQLL 1702
                                    +P      +D TE    +    ++   + +   QLL
Sbjct: 1662 QRFMDNLFLRNLTSQPKSSVAEVTVPSSPYKHEDPTEPDSREEESPALGAIRGKCITQLL 1721

Query: 1703 LIQAVMETYNMYRSHLSTKNMLILFDALHGVASHAHSINTSSALRSKLQEFASITQMQDP 1762
            L+ A+      Y S+L T   + + D L      A S N+ S LR+++    +    + P
Sbjct: 1722 LLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFASSYNSYSNLRTRMNHIPT----ERP 1737

BLAST of Cp4.1LG03g17170 vs. NCBI nr
Match: XP_023526724.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3318 bits (8603), Expect = 0.0
Identity = 1742/1802 (96.67%), Postives = 1744/1802 (96.78%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT
Sbjct: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL
Sbjct: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD
Sbjct: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS
Sbjct: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM
Sbjct: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE
Sbjct: 961  RKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSEN 1440
            EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSEN
Sbjct: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSEN 1440

Query: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500
            GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA
Sbjct: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500

Query: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAET 1560
            FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAET
Sbjct: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAET 1560

Query: 1561 NGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFD 1620
            NGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFD
Sbjct: 1561 NGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFD 1620

Query: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680
            ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH
Sbjct: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680

Query: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATRA 1740
            SYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATRA
Sbjct: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATRA 1740

Query: 1741 PLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILLR 1766
            PLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILLR
Sbjct: 1741 PLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILLR 1784

BLAST of Cp4.1LG03g17170 vs. NCBI nr
Match: KAG7018950.1 (Brefeldin A-inhibited guanine nucleotide-exchange protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3283 bits (8512), Expect = 0.0
Identity = 1722/1802 (95.56%), Postives = 1733/1802 (96.17%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPS PSSPT
Sbjct: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL
Sbjct: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD
Sbjct: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS
Sbjct: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVST+ISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTEISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQS NSNKLLGFDS
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSANSNKLLGFDS 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARK+ESVYYAATDVVILKFMIEVCWAPM
Sbjct: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKTESVYYAATDVVILKFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE
Sbjct: 961  RKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQSKSTMLPLLKKKGVGRIQYAAA VMRGSYDSAGITGN+SGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAATVMRGSYDSAGITGNSSGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWSSIWHVLS+FFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSSIWHVLSEFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLGSSSRNKDKELSGKISSLSP RAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGSSSRNKDKELSGKISSLSPRRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSEN 1440
            EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTS EQGADSEN
Sbjct: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSLEQGADSEN 1440

Query: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500
            GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA
Sbjct: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500

Query: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAET 1560
            FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDF FLVNTDSTIRSHRLELNGESNAET
Sbjct: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFMFLVNTDSTIRSHRLELNGESNAET 1560

Query: 1561 NGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFD 1620
            NGSELPDDDTESL TQHVYIS+SDAKCRAAVQLLLIQAVMETYNMYRSHLSTKN+L+LFD
Sbjct: 1561 NGSELPDDDTESLTTQHVYISISDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNVLVLFD 1620

Query: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680
            ALHGVASHAHSINTSS LRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH
Sbjct: 1621 ALHGVASHAHSINTSSTLRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680

Query: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATRA 1740
            SYEEAEVELYLVKLCHEVLQFYVETARYGLV E SASSGTQPHWPIPLGSGKQRELATRA
Sbjct: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVAEPSASSGTQPHWPIPLGSGKQRELATRA 1740

Query: 1741 PLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILLR 1766
            PLIVAILQAIC+LNEASFEKNLAGFFPLLSSLISCEHGSNEVQ+ALTEML ASVGPILLR
Sbjct: 1741 PLIVAILQAICSLNEASFEKNLAGFFPLLSSLISCEHGSNEVQVALTEMLSASVGPILLR 1784

BLAST of Cp4.1LG03g17170 vs. NCBI nr
Match: KAG6582567.1 (Brefeldin A-inhibited guanine nucleotide-exchange protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3281 bits (8506), Expect = 0.0
Identity = 1721/1802 (95.50%), Postives = 1732/1802 (96.12%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPS PS PSSPT
Sbjct: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSFPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL
Sbjct: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD
Sbjct: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS
Sbjct: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVST+ISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTEISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQS NSNKLLGFDS
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSANSNKLLGFDS 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARK+ESVYYAATDVVILKFMIEVCWAPM
Sbjct: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKTESVYYAATDVVILKFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE
Sbjct: 961  RKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQSKSTMLPLLKKKGVGRIQYAAA VMRGSYDSAGITGN+SGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAATVMRGSYDSAGITGNSSGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWSSIWHVLS+FFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSSIWHVLSEFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLGSSSRNKDKELSGKISSLSP RAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGSSSRNKDKELSGKISSLSPRRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSEN 1440
            EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTS EQGADSEN
Sbjct: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSLEQGADSEN 1440

Query: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500
            GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA
Sbjct: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500

Query: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAET 1560
            FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDF FLVNTDSTIRSHRLELNGESNAET
Sbjct: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFMFLVNTDSTIRSHRLELNGESNAET 1560

Query: 1561 NGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFD 1620
            NGSELPDDDTESL TQHVYIS+SDAKCRAAVQLLLIQAVMETYNMYRSHLSTKN+L+LFD
Sbjct: 1561 NGSELPDDDTESLTTQHVYISISDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNVLVLFD 1620

Query: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680
            ALHGVASHAHSINTSS LRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH
Sbjct: 1621 ALHGVASHAHSINTSSTLRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680

Query: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATRA 1740
            SYEEAEVELYLVKLCHEVLQFYVETARYGLV E SASSGTQPHWPIPLGSGKQRELATRA
Sbjct: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVAEPSASSGTQPHWPIPLGSGKQRELATRA 1740

Query: 1741 PLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILLR 1766
            PLIVAILQAIC+LNEASFEKNLAGFFPLLSSLISCEHGSNEVQ+ALTEML ASVGPILLR
Sbjct: 1741 PLIVAILQAICSLNEASFEKNLAGFFPLLSSLISCEHGSNEVQVALTEMLSASVGPILLR 1784

BLAST of Cp4.1LG03g17170 vs. NCBI nr
Match: XP_022924338.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 2-like [Cucurbita moschata])

HSP 1 Score: 3279 bits (8503), Expect = 0.0
Identity = 1723/1803 (95.56%), Postives = 1734/1803 (96.17%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPS PSSPT
Sbjct: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL
Sbjct: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEG LLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGNLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVV EL+EPIEKTD
Sbjct: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVTELVEPIEKTD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS
Sbjct: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVST+ISD STIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTEISDASTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDD-EMAPQLRQSTNSNKLLGFD 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDD EMAPQLRQSTNSNKLLGFD
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDDEMAPQLRQSTNSNKLLGFD 840

Query: 841  SILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAP 900
            SILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARK+ESVYYAATDVVILKFMIEVCWAP
Sbjct: 841  SILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKTESVYYAATDVVILKFMIEVCWAP 900

Query: 901  MLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA--------------------------- 960
            MLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                           
Sbjct: 901  MLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADI 960

Query: 961  ---------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNES 1020
                     AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNES
Sbjct: 961  KRKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNES 1020

Query: 1021 EKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNM 1080
            EKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGN+SGVTSEQMNNLVSNLNM
Sbjct: 1021 EKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNSSGVTSEQMNNLVSNLNM 1080

Query: 1081 LEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYN 1140
            LEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYN
Sbjct: 1081 LEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYN 1140

Query: 1141 MNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEF 1200
            MNRIRLVWSSIWHVLS+FFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEF
Sbjct: 1141 MNRIRLVWSSIWHVLSEFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEF 1200

Query: 1201 MKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLL 1260
            MKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLL
Sbjct: 1201 MKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLL 1260

Query: 1261 AFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAE 1320
            AFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAE
Sbjct: 1261 AFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAE 1320

Query: 1321 GDLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPR 1380
            GDLGSSSRNKDKELSGKISSLSP RAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPR
Sbjct: 1321 GDLGSSSRNKDKELSGKISSLSPRRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPR 1380

Query: 1381 PEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSE 1440
            PEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSE
Sbjct: 1381 PEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSE 1440

Query: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500
            NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA
Sbjct: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500

Query: 1501 AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE 1560
            AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE
Sbjct: 1501 AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE 1560

Query: 1561 TNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILF 1620
            TNGSELPDDDTESL TQHVYIS+SDAKCRAAVQLLLIQAVMETYNMYRSHLSTKN+L+LF
Sbjct: 1561 TNGSELPDDDTESLTTQHVYISISDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNVLVLF 1620

Query: 1621 DALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP 1680
            DALHGVASHAHSINTS ALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP
Sbjct: 1621 DALHGVASHAHSINTSLALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP 1680

Query: 1681 HSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATR 1740
            HSYEEAEVELYLVKLCHEVLQFYVETARYGLV EASASSGTQPHWPIPLGSGKQRELATR
Sbjct: 1681 HSYEEAEVELYLVKLCHEVLQFYVETARYGLVAEASASSGTQPHWPIPLGSGKQRELATR 1740

Query: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILL 1766
            APLIVAILQAIC LNEASFEKNLAGFFPLLSSLISCEHGSNEVQ+ALTEML ASVGPILL
Sbjct: 1741 APLIVAILQAICCLNEASFEKNLAGFFPLLSSLISCEHGSNEVQVALTEMLSASVGPILL 1785

BLAST of Cp4.1LG03g17170 vs. NCBI nr
Match: XP_022980176.1 (brefeldin A-inhibited guanine nucleotide-exchange protein 2-like [Cucurbita maxima])

HSP 1 Score: 3270 bits (8478), Expect = 0.0
Identity = 1717/1803 (95.23%), Postives = 1734/1803 (96.17%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPS PSSPT
Sbjct: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGA+PGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL
Sbjct: 61   DSEAEGALPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD
Sbjct: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGV 
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVV 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS
Sbjct: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGF+S
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFES 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKRWEDQNMETS+DLIKHMQEQFKERARK+ESVYYAATDVVILKFMIEVCWAPM
Sbjct: 841  ILNIVIRKRWEDQNMETSNDLIKHMQEQFKERARKTESVYYAATDVVILKFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE
Sbjct: 961  RKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGN+SGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNSSGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCA+KLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCASKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLGSSSRNKDKELSGKISSLSP RAKDG HDTEMTDKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGSSSRNKDKELSGKISSLSPRRAKDGIHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSEN 1440
            EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSE+GADSEN
Sbjct: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEKGADSEN 1440

Query: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500
            GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVL LLVSFIKRPHQSLAGIGIAA
Sbjct: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLMLLVSFIKRPHQSLAGIGIAA 1500

Query: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAET 1560
            FVRLMSNAGDLFSEEKWQEVVFALGE TSATLPDFTFLVNTDSTIRS+R ELNGESNAET
Sbjct: 1501 FVRLMSNAGDLFSEEKWQEVVFALGETTSATLPDFTFLVNTDSTIRSYRFELNGESNAET 1560

Query: 1561 NGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFD 1620
            NGSELPD+DTESL TQHVYIS+SDAKCRAAVQLLLIQAVMETYNMYRSHLST+N+L+LFD
Sbjct: 1561 NGSELPDNDTESLTTQHVYISISDAKCRAAVQLLLIQAVMETYNMYRSHLSTRNVLVLFD 1620

Query: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680
            ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH
Sbjct: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680

Query: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSG-TQPHWPIPLGSGKQRELATR 1740
            SYEEAEVELYLVKLCHEVLQFYVETA+YGLVVEASASSG  QPHWPIPLGSGKQRELATR
Sbjct: 1681 SYEEAEVELYLVKLCHEVLQFYVETAQYGLVVEASASSGGAQPHWPIPLGSGKQRELATR 1740

Query: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILL 1766
            APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQ+ALTEML ASVGPILL
Sbjct: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQVALTEMLSASVGPILL 1785

BLAST of Cp4.1LG03g17170 vs. ExPASy TrEMBL
Match: A0A6J1EC54 (brefeldin A-inhibited guanine nucleotide-exchange protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431861 PE=4 SV=1)

HSP 1 Score: 3279 bits (8503), Expect = 0.0
Identity = 1723/1803 (95.56%), Postives = 1734/1803 (96.17%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPS PSSPT
Sbjct: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL
Sbjct: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEG LLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGNLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVV EL+EPIEKTD
Sbjct: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVTELVEPIEKTD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS
Sbjct: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVST+ISD STIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTEISDASTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDD-EMAPQLRQSTNSNKLLGFD 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDD EMAPQLRQSTNSNKLLGFD
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDDEMAPQLRQSTNSNKLLGFD 840

Query: 841  SILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAP 900
            SILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARK+ESVYYAATDVVILKFMIEVCWAP
Sbjct: 841  SILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKTESVYYAATDVVILKFMIEVCWAP 900

Query: 901  MLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA--------------------------- 960
            MLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                           
Sbjct: 901  MLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADI 960

Query: 961  ---------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNES 1020
                     AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNES
Sbjct: 961  KRKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNES 1020

Query: 1021 EKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNM 1080
            EKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGN+SGVTSEQMNNLVSNLNM
Sbjct: 1021 EKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNSSGVTSEQMNNLVSNLNM 1080

Query: 1081 LEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYN 1140
            LEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYN
Sbjct: 1081 LEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYN 1140

Query: 1141 MNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEF 1200
            MNRIRLVWSSIWHVLS+FFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEF
Sbjct: 1141 MNRIRLVWSSIWHVLSEFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEF 1200

Query: 1201 MKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLL 1260
            MKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLL
Sbjct: 1201 MKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLL 1260

Query: 1261 AFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAE 1320
            AFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAE
Sbjct: 1261 AFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAE 1320

Query: 1321 GDLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPR 1380
            GDLGSSSRNKDKELSGKISSLSP RAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPR
Sbjct: 1321 GDLGSSSRNKDKELSGKISSLSPRRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPR 1380

Query: 1381 PEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSE 1440
            PEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSE
Sbjct: 1381 PEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSE 1440

Query: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500
            NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA
Sbjct: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500

Query: 1501 AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE 1560
            AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE
Sbjct: 1501 AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE 1560

Query: 1561 TNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILF 1620
            TNGSELPDDDTESL TQHVYIS+SDAKCRAAVQLLLIQAVMETYNMYRSHLSTKN+L+LF
Sbjct: 1561 TNGSELPDDDTESLTTQHVYISISDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNVLVLF 1620

Query: 1621 DALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP 1680
            DALHGVASHAHSINTS ALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP
Sbjct: 1621 DALHGVASHAHSINTSLALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP 1680

Query: 1681 HSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATR 1740
            HSYEEAEVELYLVKLCHEVLQFYVETARYGLV EASASSGTQPHWPIPLGSGKQRELATR
Sbjct: 1681 HSYEEAEVELYLVKLCHEVLQFYVETARYGLVAEASASSGTQPHWPIPLGSGKQRELATR 1740

Query: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILL 1766
            APLIVAILQAIC LNEASFEKNLAGFFPLLSSLISCEHGSNEVQ+ALTEML ASVGPILL
Sbjct: 1741 APLIVAILQAICCLNEASFEKNLAGFFPLLSSLISCEHGSNEVQVALTEMLSASVGPILL 1785

BLAST of Cp4.1LG03g17170 vs. ExPASy TrEMBL
Match: A0A6J1ISX0 (brefeldin A-inhibited guanine nucleotide-exchange protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479644 PE=4 SV=1)

HSP 1 Score: 3270 bits (8478), Expect = 0.0
Identity = 1717/1803 (95.23%), Postives = 1734/1803 (96.17%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPS PSSPT
Sbjct: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGA+PGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL
Sbjct: 61   DSEAEGALPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD
Sbjct: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGV 
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVV 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS
Sbjct: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGF+S
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFES 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKRWEDQNMETS+DLIKHMQEQFKERARK+ESVYYAATDVVILKFMIEVCWAPM
Sbjct: 841  ILNIVIRKRWEDQNMETSNDLIKHMQEQFKERARKTESVYYAATDVVILKFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE
Sbjct: 961  RKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGN+SGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNSSGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCA+KLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCASKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLGSSSRNKDKELSGKISSLSP RAKDG HDTEMTDKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGSSSRNKDKELSGKISSLSPRRAKDGIHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSEN 1440
            EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSE+GADSEN
Sbjct: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEKGADSEN 1440

Query: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAA 1500
            GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVL LLVSFIKRPHQSLAGIGIAA
Sbjct: 1441 GELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLMLLVSFIKRPHQSLAGIGIAA 1500

Query: 1501 FVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAET 1560
            FVRLMSNAGDLFSEEKWQEVVFALGE TSATLPDFTFLVNTDSTIRS+R ELNGESNAET
Sbjct: 1501 FVRLMSNAGDLFSEEKWQEVVFALGETTSATLPDFTFLVNTDSTIRSYRFELNGESNAET 1560

Query: 1561 NGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFD 1620
            NGSELPD+DTESL TQHVYIS+SDAKCRAAVQLLLIQAVMETYNMYRSHLST+N+L+LFD
Sbjct: 1561 NGSELPDNDTESLTTQHVYISISDAKCRAAVQLLLIQAVMETYNMYRSHLSTRNVLVLFD 1620

Query: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680
            ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH
Sbjct: 1621 ALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPH 1680

Query: 1681 SYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSG-TQPHWPIPLGSGKQRELATR 1740
            SYEEAEVELYLVKLCHEVLQFYVETA+YGLVVEASASSG  QPHWPIPLGSGKQRELATR
Sbjct: 1681 SYEEAEVELYLVKLCHEVLQFYVETAQYGLVVEASASSGGAQPHWPIPLGSGKQRELATR 1740

Query: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILL 1766
            APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQ+ALTEML ASVGPILL
Sbjct: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQVALTEMLSASVGPILL 1785

BLAST of Cp4.1LG03g17170 vs. ExPASy TrEMBL
Match: A0A1S3AVC3 (brefeldin A-inhibited guanine nucleotide-exchange protein 2-like OS=Cucumis melo OX=3656 GN=LOC103483344 PE=4 SV=1)

HSP 1 Score: 3162 bits (8199), Expect = 0.0
Identity = 1654/1803 (91.74%), Postives = 1706/1803 (94.62%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQV++PALEKI+KNASWRKHSKLAHECKSVIERL SSPKPSSPS PSSPT
Sbjct: 1    MASSEAASRLSQVVSPALEKIIKNASWRKHSKLAHECKSVIERLTSSPKPSSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGAVPGPLNDGGPDE+SLAESE+ILSPLINASSSGVLKIADPAVDCIQKLIA+GYL
Sbjct: 61   DSEAEGAVPGPLNDGGPDEYSLAESEAILSPLINASSSGVLKIADPAVDCIQKLIAHGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNV+NQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEK D
Sbjct: 181  VKTCYDIYLDSKNVVNQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKAD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKI+QDIDGVLNP TPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIMQDIDGVLNPTTPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGE ERDDDLE+QIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEVERDDDLEVQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLC+DSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA
Sbjct: 481  QPNFQQKMIVLRFVEKLCIDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMK EAMKCLVAILKSMGDWLN+QLRIPDPHSTKKIEVAE +SES +VP+S
Sbjct: 541  TTLLPPQELTMKHEAMKCLVAILKSMGDWLNKQLRIPDPHSTKKIEVAEANSESVSVPMS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVST+ SDV TIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTETSDVLTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDK+LIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKTLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDE+APQ RQSTNSNKLLGFDS
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDELAPQQRQSTNSNKLLGFDS 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKR EDQNMETSDDLI+HMQEQFKE+ARK+ESVYYAATDVVIL+FMIEVCWAPM
Sbjct: 841  ILNIVIRKRGEDQNMETSDDLIRHMQEQFKEKARKTESVYYAATDVVILRFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEG+FLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE
Sbjct: 961  QKNIDAIKAIVKIADEEGDFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQSK+TMLP+LKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQSKATMLPVLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVS+EELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSVEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWS IWHVLSDFFV+IGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSRIWHVLSDFFVSIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAA DDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLGSSSRNKDKELSGK + LSP +AKDGKHD EM DKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGSSSRNKDKELSGKSTPLSPQKAKDGKHDAEMNDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSS-TSSEQGADSE 1440
            EIRKSALQVLFDTLRKHGH FSLPLWERVFESVLFPIFDYVRHAIDPSS +SSEQG DSE
Sbjct: 1381 EIRKSALQVLFDTLRKHGHLFSLPLWERVFESVLFPIFDYVRHAIDPSSASSSEQGVDSE 1440

Query: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500
            NGELDQDAWLYETCTLALQLVVDLFVKFY TVNPLLKKVLTLLVSFIKRPHQSLAGIGIA
Sbjct: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYGTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500

Query: 1501 AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE 1560
            AFVRLMSNAGDLFSEEKWQEVVF+L EAT+ATLPDFTFL+NT+STIRSHR+ELN E+NAE
Sbjct: 1501 AFVRLMSNAGDLFSEEKWQEVVFSLKEATTATLPDFTFLMNTNSTIRSHRVELNEENNAE 1560

Query: 1561 TNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILF 1620
            TNGSELP+DD+ESL  QHVY S+SDAKCRAAVQLLLIQAVME YNMYRSHLSTKN+L+LF
Sbjct: 1561 TNGSELPEDDSESLTVQHVYTSISDAKCRAAVQLLLIQAVMEIYNMYRSHLSTKNVLVLF 1620

Query: 1621 DALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP 1680
            DALH VASHAHSINTS  +R+KLQEFASITQMQDPPLLRLENESYQICL+FVQNLI+D+P
Sbjct: 1621 DALHSVASHAHSINTSGPIRTKLQEFASITQMQDPPLLRLENESYQICLSFVQNLIVDRP 1680

Query: 1681 HSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATR 1740
            H+YEEAEVELYL+KLC EVLQFYVETA+YG VVEAS SSGTQPHW IPLGSGK+RELA R
Sbjct: 1681 HNYEEAEVELYLIKLCREVLQFYVETAQYGCVVEASVSSGTQPHWAIPLGSGKRRELAAR 1740

Query: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILL 1766
            APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLAL+EML  SVGPILL
Sbjct: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALSEMLNTSVGPILL 1785

BLAST of Cp4.1LG03g17170 vs. ExPASy TrEMBL
Match: A0A0A0L924 (SEC7 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G126070 PE=4 SV=1)

HSP 1 Score: 3155 bits (8181), Expect = 0.0
Identity = 1651/1803 (91.57%), Postives = 1701/1803 (94.34%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLSQV++PALEKI+KNASWRKHSKLAHECKSVIERL SSPKPSSPS PSSPT
Sbjct: 1    MASSEAASRLSQVVSPALEKIIKNASWRKHSKLAHECKSVIERLTSSPKPSSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSEAEGAVPGPLNDGGPDE+SLAESE+ILSPLINASSSGVLKIADPAVDCIQKLIA+GYL
Sbjct: 61   DSEAEGAVPGPLNDGGPDEYSLAESEAILSPLINASSSGVLKIADPAVDCIQKLIAHGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGGVEGKLL+KLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGVEGKLLSKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNV+NQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEK D
Sbjct: 181  VKTCYDIYLDSKNVVNQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKAD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKI+QDIDGVLNP TPGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIMQDIDGVLNPTTPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGE ERDDDLE+QIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEVERDDDLEVQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLC+DSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPG A
Sbjct: 481  QPNFQQKMIVLRFVEKLCIDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGAA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMK EAMKCLVAILKSMGDWLN+QLRIPDPHSTKKIEV E SSES +VP+S
Sbjct: 541  TTLLPPQELTMKHEAMKCLVAILKSMGDWLNKQLRIPDPHSTKKIEVTEASSESVSVPMS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGTTDEHGEGSDSHSEVST+ SDV TIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTTDEHGEGSDSHSEVSTETSDVLTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL
Sbjct: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDE+APQ RQSTNSNKLLGFDS
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDELAPQQRQSTNSNKLLGFDS 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKR EDQNMETSDDLI+HMQEQFKE+ARK+ESVYYAATDVVIL+FMIEVCWAPM
Sbjct: 841  ILNIVIRKRGEDQNMETSDDLIRHMQEQFKEKARKTESVYYAATDVVILRFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNES+
Sbjct: 961  QKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESD 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQSK+TMLP+LKKKGVGRIQ+AAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQSKATMLPVLKKKGVGRIQFAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVS+EELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSVEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWS IWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSRIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAA DDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLGSSSRNKDKELSGK S LSP +AKDGKHD EM DKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGSSSRNKDKELSGKSSPLSPQKAKDGKHDAEMNDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSS-TSSEQGADSE 1440
            EIRKSALQVLFDTLRKHGH FSLPLWERVFESVLFPIFDYVRHAIDPSS +SSEQG DSE
Sbjct: 1381 EIRKSALQVLFDTLRKHGHLFSLPLWERVFESVLFPIFDYVRHAIDPSSASSSEQGVDSE 1440

Query: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500
            NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA
Sbjct: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500

Query: 1501 AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE 1560
            AFVRLMSNAGDLFSEEKWQEVVF+L EAT+ATLPDF FL+NT+STIRSHR+E N E+NAE
Sbjct: 1501 AFVRLMSNAGDLFSEEKWQEVVFSLKEATTATLPDFIFLLNTNSTIRSHRVESNEENNAE 1560

Query: 1561 TNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILF 1620
            TNGSELP+DD+ESL  QHVY S+SDAKCRAAVQLLLIQAVME YNMYRSHLSTKN+L+LF
Sbjct: 1561 TNGSELPEDDSESLTVQHVYTSISDAKCRAAVQLLLIQAVMEIYNMYRSHLSTKNVLVLF 1620

Query: 1621 DALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP 1680
            DALH VASHAH INTS  +R+KLQEFASITQMQDPPLLRLENESYQICL+FVQNLI+D+P
Sbjct: 1621 DALHSVASHAHVINTSGPIRTKLQEFASITQMQDPPLLRLENESYQICLSFVQNLIVDRP 1680

Query: 1681 HSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATR 1740
            HSYEEAEVELYL+KLCHEVLQFYVETA+YG VVEAS SSGTQPHW IPLGSGK+RELA R
Sbjct: 1681 HSYEEAEVELYLIKLCHEVLQFYVETAQYGCVVEASVSSGTQPHWAIPLGSGKRRELAAR 1740

Query: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILL 1766
            APLIVAILQAICNL+EASFEKNL G FPLLSSLISCEHGSNEVQLAL+EML  SVGPILL
Sbjct: 1741 APLIVAILQAICNLSEASFEKNLTGLFPLLSSLISCEHGSNEVQLALSEMLNTSVGPILL 1785

BLAST of Cp4.1LG03g17170 vs. ExPASy TrEMBL
Match: A0A6J1F2G4 (brefeldin A-inhibited guanine nucleotide-exchange protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111441526 PE=4 SV=1)

HSP 1 Score: 3132 bits (8119), Expect = 0.0
Identity = 1639/1803 (90.90%), Postives = 1694/1803 (93.95%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEAASRLS VI+PALEKI+KNASWRKHSKLA+ECKSV+ER  SS K SSPS PSSPT
Sbjct: 1    MASSEAASRLSLVISPALEKIIKNASWRKHSKLANECKSVLERFTSSSKASSPSSPSSPT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
             SEAEG++PGPLNDGGP E+SL ESESILSPL+NASSSGVLKIADPAVDCIQKLIA+GYL
Sbjct: 61   GSEAEGSLPGPLNDGGPIEYSLVESESILSPLMNASSSGVLKIADPAVDCIQKLIAHGYL 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADPSGG EGKLLAKLIESVCKCHDLGDDALELLVLK LLSAVTSISLRIHGDCLLQI
Sbjct: 121  RGEADPSGGAEGKLLAKLIESVCKCHDLGDDALELLVLKALLSAVTSISLRIHGDCLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            VKTCYDIYLDSKNV+NQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEK D
Sbjct: 181  VKTCYDIYLDSKNVVNQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKAD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            ADGSMTQFVQGFITKI+QDIDGVLNPA PGKVSIGAHDGAFETTTVETTNPADLLDSTDK
Sbjct: 241  ADGSMTQFVQGFITKIMQDIDGVLNPAIPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLE+QIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEVQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKEAMADPQLMKGKIVALELLKILLENAG               +  T +    RFLG
Sbjct: 361  TPPKEAMADPQLMKGKIVALELLKILLENAG--------------AVFRTSE----RFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA
Sbjct: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQELTMKLE+MKCLVAILKSMGDWLN+QLRIPDPHSTKKIEVAENSSE+ +VP+S
Sbjct: 541  TTLLPPQELTMKLESMKCLVAILKSMGDWLNKQLRIPDPHSTKKIEVAENSSEAVSVPMS 600

Query: 601  NGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660
            NGT+DEHGEGSDSHSEVST+ S+V TIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK
Sbjct: 601  NGTSDEHGEGSDSHSEVSTESSEVLTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANK 660

Query: 661  VGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALL 720
            VGSSPEEIAAFLKDASGLDK+LIGDYLGEREDLSLKVMHAYVDSFDF+GLEFDEAIR  L
Sbjct: 661  VGSSPEEIAAFLKDASGLDKALIGDYLGEREDLSLKVMHAYVDSFDFRGLEFDEAIRVFL 720

Query: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780
            KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM
Sbjct: 721  KGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKM 780

Query: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDS 840
            SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDE+APQ +QSTNSNKLLGFDS
Sbjct: 781  SAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDELAPQQKQSTNSNKLLGFDS 840

Query: 841  ILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPM 900
            ILNIVIRKR EDQNMETSDDLI+HMQEQFKE+ARK+ESVYYAATDVVIL+FMIEVCWAPM
Sbjct: 841  ILNIVIRKRGEDQNMETSDDLIRHMQEQFKEKARKTESVYYAATDVVILRFMIEVCWAPM 900

Query: 901  LAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------------- 960
            LAAFSVPLDRSDD+V+IALCLEGFQYAIHVTA                            
Sbjct: 901  LAAFSVPLDRSDDDVVIALCLEGFQYAIHVTAVMSMKTHRDAFVTSLAKFTSLHSPADIK 960

Query: 961  --------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020
                    AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE
Sbjct: 961  QKNIDAIKAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESE 1020

Query: 1021 KSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080
            KSKQ+K TMLPLLKKKG+GRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML
Sbjct: 1021 KSKQTKGTMLPLLKKKGLGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMNNLVSNLNML 1080

Query: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNM 1140
            EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVS+EELRSTSDPRVFSLTKIVEIAHYNM
Sbjct: 1081 EQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSVEELRSTSDPRVFSLTKIVEIAHYNM 1140

Query: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200
            NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM
Sbjct: 1141 NRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFM 1200

Query: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLA 1260
            KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAA DDHKNIVLLA
Sbjct: 1201 KPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNIVLLA 1260

Query: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320
            FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG
Sbjct: 1261 FEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEG 1320

Query: 1321 DLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380
            DLG SSRNKDKEL+GK SSLSP RAKDGKHD EMTDKDNHLYFWFPLLAGLSELSFDPRP
Sbjct: 1321 DLGFSSRNKDKELAGKSSSLSPRRAKDGKHDAEMTDKDNHLYFWFPLLAGLSELSFDPRP 1380

Query: 1381 EIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSS-TSSEQGADSE 1440
            EIRKSALQVLFDTLRKHGH FSLPLWERVFESVLFPIFDYVRHAIDPSS +SSEQG DSE
Sbjct: 1381 EIRKSALQVLFDTLRKHGHLFSLPLWERVFESVLFPIFDYVRHAIDPSSASSSEQGMDSE 1440

Query: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500
            NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA
Sbjct: 1441 NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIA 1500

Query: 1501 AFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAE 1560
            AFVRLMSNAGDLFSEEKWQEVVF+L EAT+ATLPDFTFLV++DSTIRSHR+EL GE NAE
Sbjct: 1501 AFVRLMSNAGDLFSEEKWQEVVFSLKEATTATLPDFTFLVDSDSTIRSHRIELKGEGNAE 1560

Query: 1561 TNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILF 1620
            +NGSELP DD+ESL  QHVY S+SDAKCRAAVQLLLIQAVME YNMYRSHLSTKN+L+LF
Sbjct: 1561 SNGSELPYDDSESLTVQHVYTSISDAKCRAAVQLLLIQAVMEIYNMYRSHLSTKNVLVLF 1620

Query: 1621 DALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKP 1680
            DALH VASHAH+IN SSA+RSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLI+D P
Sbjct: 1621 DALHSVASHAHNINASSAIRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLIVDGP 1680

Query: 1681 HSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATR 1740
            H YEEAEVE  L KLCHEVLQFY+ETARYG V EAS SSGTQPHWPIPLGSGK+RELA R
Sbjct: 1681 HDYEEAEVESCLTKLCHEVLQFYIETARYGNVAEASVSSGTQPHWPIPLGSGKRRELAAR 1740

Query: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPILL 1766
            APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQ AL++ML  +VGPILL
Sbjct: 1741 APLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQAALSDMLSTTVGPILL 1785

BLAST of Cp4.1LG03g17170 vs. TAIR 10
Match: AT3G60860.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 2537.3 bits (6575), Expect = 0.0e+00
Identity = 1339/1814 (73.81%), Postives = 1510/1814 (83.24%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MASSEA SRLS+V+TPALEKIVKNASWRKHSKLA+ECK+VIERL S  K S P   S+ T
Sbjct: 1    MASSEADSRLSRVVTPALEKIVKNASWRKHSKLANECKAVIERLNSLQK-SPPPSSSAAT 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSE+E +VPGPLNDGG  E+SLA+SE I SPLINA  +G+ KI +PA+DCIQKLIA+GY+
Sbjct: 61   DSESESSVPGPLNDGGSIEYSLADSELIFSPLINACGTGLAKIIEPAIDCIQKLIAHGYI 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGE+DPSGG E  LL KLI+SVCKCHDLGD+++EL VLKTLLSA+ SISLRIHG CLL +
Sbjct: 121  RGESDPSGGAESLLLFKLIDSVCKCHDLGDESIELPVLKTLLSAINSISLRIHGKCLLLV 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            V+TCYDIYL SKNV+NQTTAKASLIQ+LVIVFRRMEADSSTVP+QPIVVAELMEP+EK+D
Sbjct: 181  VRTCYDIYLGSKNVVNQTTAKASLIQILVIVFRRMEADSSTVPIQPIVVAELMEPLEKSD 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVS---IGAHDGAFETTTVETTNPADLLDS 300
            ADG+MTQFVQGFITKI+QDIDGVLNP   G  S    G  DGA+ TTTVETTNP DLLDS
Sbjct: 241  ADGTMTQFVQGFITKIMQDIDGVLNPTMSGSGSGSGSGGQDGAYGTTTVETTNPTDLLDS 300

Query: 301  TDKDMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKL 360
            TDKDMLDAKYWEISMYK+ALEGRKGEL DG+ ERDDDLE+QI NKLRRDA LVFRALCKL
Sbjct: 301  TDKDMLDAKYWEISMYKSALEGRKGELTDGDAERDDDLEVQIENKLRRDACLVFRALCKL 360

Query: 361  SMKTPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNR 420
            SMK PPKE+ ADPQ M+GKI+ALELLKILLENAG               +  T +    +
Sbjct: 361  SMKAPPKESSADPQSMRGKILALELLKILLENAG--------------AVFRTSE----K 420

Query: 421  FLGAIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLE 480
            F   IKQ+LCLSLLKNSASTLMIIFQLSCSIFISLV+RFRAGLKAEIGVFFPMIVLRV+E
Sbjct: 421  FSADIKQFLCLSLLKNSASTLMIIFQLSCSIFISLVARFRAGLKAEIGVFFPMIVLRVVE 480

Query: 481  NVAQPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPP 540
            NVAQPNFQQKMIVLRF++KLC+DSQILVDIF+NYDCDVNSSNIFERMVNGLLKTAQGVPP
Sbjct: 481  NVAQPNFQQKMIVLRFLDKLCLDSQILVDIFLNYDCDVNSSNIFERMVNGLLKTAQGVPP 540

Query: 541  GVATTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNV 600
            G ATTL+PPQE  MKLEAMKCLVAILKSMGDWLN+QLR+P  +S  K +V E     G+ 
Sbjct: 541  GTATTLMPPQEAAMKLEAMKCLVAILKSMGDWLNKQLRLPVSNSLNKSDVIEIDLGPGSP 600

Query: 601  PISNGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLIN 660
             ++NG  DE  +GSD++SE S   SD   IEQRRAYKLELQEGISLFNRKP KGIEFLIN
Sbjct: 601  QLANGNADESADGSDTYSESSGGTSDALAIEQRRAYKLELQEGISLFNRKPTKGIEFLIN 660

Query: 661  ANKVGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIR 720
            A KVG SPEEIA FLKDASGL+K+LIGDYLGEREDL+LKVMHAYVDSFDF+G+EFDEAIR
Sbjct: 661  AGKVGESPEEIAGFLKDASGLNKTLIGDYLGEREDLALKVMHAYVDSFDFRGMEFDEAIR 720

Query: 721  ALLKGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVK 780
              L+GFRLPGEAQKIDRIMEKFAERYCKCNPK F SAD+AYVLAYSVI+LNTDAHNPMVK
Sbjct: 721  TFLEGFRLPGEAQKIDRIMEKFAERYCKCNPKVFTSADSAYVLAYSVIMLNTDAHNPMVK 780

Query: 781  NKMSAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLG 840
            NKMSA+DFIRNNRGIDDGKDLP +Y++SLYERI+++EIKMK+D++  Q +Q  NSN++LG
Sbjct: 781  NKMSADDFIRNNRGIDDGKDLPADYMRSLYERITKHEIKMKEDDLRLQQKQYANSNRMLG 840

Query: 841  FDSILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCW 900
             D ILNIVIRK+W D   ETSDDL+KHMQEQFKE+ARKSES YYAATDVVIL+FMIE CW
Sbjct: 841  LDGILNIVIRKQWGDSYAETSDDLMKHMQEQFKEKARKSESTYYAATDVVILRFMIEACW 900

Query: 901  APMLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA------------------------- 960
            APMLAAFSVPLD+SDD ++I +CLEGF +AIH T+                         
Sbjct: 901  APMLAAFSVPLDQSDDLIVINICLEGFHHAIHATSLMSMKTHRDAFVTSLAKFTSLHSPA 960

Query: 961  -----------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQN 1020
                       AI+++ADEEGN+LQ+AWEHILTCVSRFE LHLLGEGAPPDATFFA  QN
Sbjct: 961  DIKQRNIEAIKAILRLADEEGNYLQDAWEHILTCVSRFEQLHLLGEGAPPDATFFASKQN 1020

Query: 1021 ESEKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNAS-GVTSEQMNNLVSN 1080
            ESEKSKQ K  +LP+LK+KG G+ QYAA  V+RGSYDS  + G  S  V  EQM+++VSN
Sbjct: 1021 ESEKSKQPKQYILPVLKRKGPGKSQYAATGVLRGSYDSMSLGGKGSKNVRQEQMSSIVSN 1080

Query: 1081 LNMLEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIA 1140
            LN+LEQVG  EMN++F++SQKLNSEAI+DFVKALCKVSM+ELRS S+PRVFSLTKIVEIA
Sbjct: 1081 LNLLEQVG--EMNQVFSQSQKLNSEAIIDFVKALCKVSMDELRSPSNPRVFSLTKIVEIA 1140

Query: 1141 HYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQ 1200
            HYNMNRIRLVWSSIW VLS FFVTIGCSENLSIAIFAMDSLRQLSMKFL+REELANYNFQ
Sbjct: 1141 HYNMNRIRLVWSSIWQVLSGFFVTIGCSENLSIAIFAMDSLRQLSMKFLEREELANYNFQ 1200

Query: 1201 NEFMKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNI 1260
            NEFM PFVIVMR+S+ VEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAA DDHKNI
Sbjct: 1201 NEFMTPFVIVMRRSNDVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAAYDDHKNI 1260

Query: 1261 VLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATK 1320
            V L+FEIIEKIIR+YFPYITETETTTFTDCVNCL+AFTNNRF+KDISL++IAFLR+CATK
Sbjct: 1261 VFLSFEIIEKIIREYFPYITETETTTFTDCVNCLVAFTNNRFSKDISLSSIAFLRYCATK 1320

Query: 1321 LAEGDLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSELSF 1380
            LAEGDL S S NK K  SGKI   S    K GK +      +NHLYFWFPLL+GLSELSF
Sbjct: 1321 LAEGDLNSPSTNKYKGTSGKIPQSSLHSGKSGKQENGEIVNNNHLYFWFPLLSGLSELSF 1380

Query: 1381 DPRPEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPS--STSSEQ 1440
            DPRPEIRKSALQ++FDTLR HGH FSLPLWE+VFESVLFPIFDYVRH+IDPS    S++Q
Sbjct: 1381 DPRPEIRKSALQIMFDTLRNHGHLFSLPLWEKVFESVLFPIFDYVRHSIDPSGEDESADQ 1440

Query: 1441 GAD-SENGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSL 1500
            G+   E  ELD DAWLYETCTLALQLVVDLFVKFY+TVNPLL+KVL LLVSFIKRPHQSL
Sbjct: 1441 GSSGGEVDELDHDAWLYETCTLALQLVVDLFVKFYTTVNPLLEKVLMLLVSFIKRPHQSL 1500

Query: 1501 AGIGIAAFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELN 1560
            AGIGIAAFVRLMS+A  LFSEEKW EVV AL EA   T PDF++ ++ +   RS R  LN
Sbjct: 1501 AGIGIAAFVRLMSDADGLFSEEKWLEVVSALKEAAKTTCPDFSYFLSEEYVARSQRSALN 1560

Query: 1561 -GESNAETNGSELPDDDTESLRT-QHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLS 1620
               SNAE+      D + ES RT  H+Y ++SDAKCRAAVQLLLIQAVME YNMYR  LS
Sbjct: 1561 IQNSNAESAAPTATDGNEESQRTATHLYAAISDAKCRAAVQLLLIQAVMEIYNMYRPQLS 1620

Query: 1621 TKNMLILFDALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFV 1680
             KN L+L DALHGVA HAH IN+++ LRS+LQE   +TQMQDPPLLRLENESYQICLTF+
Sbjct: 1621 AKNTLVLVDALHGVALHAHGINSNTILRSRLQELGPMTQMQDPPLLRLENESYQICLTFL 1680

Query: 1681 QNLILD---KPHSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPL 1740
            QNL+ D   K    EE E+E  LV +C EVL FY+ET+     +++ +S  ++  W IPL
Sbjct: 1681 QNLVADKTKKEEEEEEEEIESLLVNICQEVLNFYIETSSSAKKLQSESSRASEYRWRIPL 1740

Query: 1741 GSGKQRELATRAPLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTE 1767
            GSGK+REL+ RAPLIVA LQA+C L+EASFEKNL   FPLL++LISCEHGSNEVQ AL +
Sbjct: 1741 GSGKRRELSARAPLIVATLQAMCTLDEASFEKNLKCLFPLLANLISCEHGSNEVQTALAD 1793

BLAST of Cp4.1LG03g17170 vs. TAIR 10
Match: AT1G01960.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 2424.8 bits (6283), Expect = 0.0e+00
Identity = 1280/1807 (70.84%), Postives = 1475/1807 (81.63%), Query Frame = 0

Query: 1    MASSEAASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPT 60
            MAS+E  SRL +V+ PAL+K++KNASWRKHSKLAHECKSVIERL  SP+ SSP       
Sbjct: 1    MASTEVDSRLGRVVIPALDKVIKNASWRKHSKLAHECKSVIERL-RSPENSSP-----VA 60

Query: 61   DSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYL 120
            DSE+  ++PGPL+DGG  E+SLAESE ILSPLINASS+GVLKI DPAVDCIQKLIA+GY+
Sbjct: 61   DSESGSSIPGPLHDGGAAEYSLAESEIILSPLINASSTGVLKIVDPAVDCIQKLIAHGYV 120

Query: 121  RGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQI 180
            RGEADP+GG E  LL+KLIE++CKCH+L D+ LELLVLKTLL+AVTSISLRIHGD LLQI
Sbjct: 121  RGEADPTGGPEALLLSKLIETICKCHELDDEGLELLVLKTLLTAVTSISLRIHGDSLLQI 180

Query: 181  VKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTD 240
            V+TCY IYL S+NV+NQ TAKASL+QM VIVFRRMEADSSTVP+QPIVVAELMEP++K++
Sbjct: 181  VRTCYGIYLGSRNVVNQATAKASLVQMSVIVFRRMEADSSTVPIQPIVVAELMEPMDKSE 240

Query: 241  ADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDK 300
            +D S TQ VQGFITKI+QDIDGV N A   K + G HDGAFET+   T NP DLLDSTDK
Sbjct: 241  SDPSTTQSVQGFITKIMQDIDGVFNSAN-AKGTFGGHDGAFETSLPGTANPTDLLDSTDK 300

Query: 301  DMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMK 360
            DMLDAKYWEISMYK+ALEGRKGELADGE E+DDD E+QIGNKLRRDAFLVFRALCKLSMK
Sbjct: 301  DMLDAKYWEISMYKSALEGRKGELADGEVEKDDDSEVQIGNKLRRDAFLVFRALCKLSMK 360

Query: 361  TPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLG 420
            TPPKE   DP+LM+GKIVALELLKILLENAG               +  T     +RFLG
Sbjct: 361  TPPKE---DPELMRGKIVALELLKILLENAG--------------AVFRTS----DRFLG 420

Query: 421  AIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480
            AIKQYLCLSLLKNSAS LMIIFQLSCSI +SLVSRFRAGLKAEIGVFFPMIVLRVLENVA
Sbjct: 421  AIKQYLCLSLLKNSASNLMIIFQLSCSILLSLVSRFRAGLKAEIGVFFPMIVLRVLENVA 480

Query: 481  QPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVA 540
            QP+FQQKMIVLRF++KLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPG  
Sbjct: 481  QPDFQQKMIVLRFLDKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGTV 540

Query: 541  TTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSESGNVPIS 600
            TTLLPPQE  MKLEAMKCLVA+L+SMGDW+N+QLR+PDP+S K +E+ + + E G+ P+ 
Sbjct: 541  TTLLPPQEAAMKLEAMKCLVAVLRSMGDWVNKQLRLPDPYSAKMLEIVDRNLEEGSHPVE 600

Query: 601  NGTTD-EHG--EGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLIN 660
            NG  D  HG  E SDS SE+S+  SD   IEQRRAYKLELQEGIS+FN+KPKKGIEFLI 
Sbjct: 601  NGKGDGGHGGFERSDSQSELSSGNSDALAIEQRRAYKLELQEGISIFNQKPKKGIEFLIK 660

Query: 661  ANKVGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIR 720
            ANKVG SPEEIAAFLKDASGL+K+LIGDYLGEREDLSLKVMHAYVDSF+FQG+EFDEAIR
Sbjct: 661  ANKVGDSPEEIAAFLKDASGLNKTLIGDYLGEREDLSLKVMHAYVDSFEFQGMEFDEAIR 720

Query: 721  ALLKGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVK 780
            A L+GFRLPGEAQKIDRIMEKFAER+CKCNPK F SADTAYVLAYSVILLNTDAHNPMVK
Sbjct: 721  AFLRGFRLPGEAQKIDRIMEKFAERFCKCNPKDFSSADTAYVLAYSVILLNTDAHNPMVK 780

Query: 781  NKMSAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLG 840
            +KM+A+ FIRNNRGIDDGKDLPEEYL++LYERISRNEIKMKDD + PQ +Q TNS++LLG
Sbjct: 781  SKMTADGFIRNNRGIDDGKDLPEEYLRALYERISRNEIKMKDDGLGPQQKQPTNSSRLLG 840

Query: 841  FDSILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCW 900
             D+ILNIV+ +R +D NMETSDDLI+HMQE+FKE+ARKSESVYYAA+DV+IL+FM+EVCW
Sbjct: 841  LDTILNIVVPRRGDDMNMETSDDLIRHMQERFKEKARKSESVYYAASDVIILRFMVEVCW 900

Query: 901  APMLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA------------------------- 960
            APMLAAFSVPLD+SDD VI  LCLEGF +AIHVT+                         
Sbjct: 901  APMLAAFSVPLDQSDDAVITTLCLEGFHHAIHVTSVMSLKTHRDAFVTSLAKFTSLHSPA 960

Query: 961  -----------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQN 1020
                       AIVK+A+EEGN+LQ+AWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQ 
Sbjct: 961  DIKQKNIEAIKAIVKLAEEEGNYLQDAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQT 1020

Query: 1021 ESEKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASG-VTSEQMNNLVSN 1080
            ES  S  +K   +P +K++  G++QYAA+A++RGSYD +G+ G AS  VTSEQMNNL+SN
Sbjct: 1021 ESGNSPLAKPNSVPAIKERAPGKLQYAASAMIRGSYDGSGVAGKASNTVTSEQMNNLISN 1080

Query: 1081 LNMLEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIA 1140
            LN+LEQVG  +M+RIFTRSQ+LNSEAI+DFVKALCKVSM+ELRS SDPRVFSLTKIVEIA
Sbjct: 1081 LNLLEQVG--DMSRIFTRSQRLNSEAIIDFVKALCKVSMDELRSPSDPRVFSLTKIVEIA 1140

Query: 1141 HYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQ 1200
            HYNMNRIRLVWSSIWHVLSDFFVTIGCS+NLSIAIFAMDSLRQLSMKFL+REELANYNFQ
Sbjct: 1141 HYNMNRIRLVWSSIWHVLSDFFVTIGCSDNLSIAIFAMDSLRQLSMKFLEREELANYNFQ 1200

Query: 1201 NEFMKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNI 1260
            NEFMKPFV+VMRKS AVEIRELIIRCVSQMVLSRV+NVKSGWKSMFM+FTTAA D HKNI
Sbjct: 1201 NEFMKPFVVVMRKSGAVEIRELIIRCVSQMVLSRVDNVKSGWKSMFMIFTTAAHDAHKNI 1260

Query: 1261 VLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATK 1320
            V L+FE++EKIIRDYFP+ITETETTTFTDCVNCL+AFTN +F KDISL AIAFL++CA K
Sbjct: 1261 VFLSFEMVEKIIRDYFPHITETETTTFTDCVNCLVAFTNCKFEKDISLQAIAFLQYCARK 1320

Query: 1321 LAEGDLGSSSRNKDKELSGKISSLSPLRAKDGKHDT-EMTDKDNHLYFWFPLLAGLSELS 1380
            LAEG +GSS R            LSP   K GK D+ +  + D HLY WFPLLAGLSELS
Sbjct: 1321 LAEGYVGSSLRRN--------PPLSPQGGKIGKQDSGKFLESDEHLYSWFPLLAGLSELS 1380

Query: 1381 FDPRPEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQG 1440
            FDPR EIRK AL+VLFDTLR HG HFSL LWERVFESVLF IFDYVR  +DPS   S   
Sbjct: 1381 FDPRAEIRKVALKVLFDTLRNHGDHFSLALWERVFESVLFRIFDYVRQDVDPSEDDSTD- 1440

Query: 1441 ADSENGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAG 1500
                NGE+DQ++WLYETC+LALQLVVDLFV FY TVNPLLKKVL L VS IKRPHQSLAG
Sbjct: 1441 QRGYNGEVDQESWLYETCSLALQLVVDLFVNFYKTVNPLLKKVLMLFVSLIKRPHQSLAG 1500

Query: 1501 IGIAAFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGE 1560
             GIAA VRLM + G  FS E+W EVV  + EA  AT PDF+++ + D       L  +  
Sbjct: 1501 AGIAALVRLMRDVGHQFSNEQWLEVVSCIKEAADATSPDFSYVTSED-------LMEDVS 1560

Query: 1561 SNAETNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNM 1620
            +  ETN +    +D    R + ++  ++DAK +A++Q+ +IQAV + Y+MYR  L+  +M
Sbjct: 1561 NEDETNDN---SNDALRRRNRQLHAVVTDAKSKASIQIFVIQAVTDIYDMYRMSLTANHM 1620

Query: 1621 LILFDALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLI 1680
            L+LFDA+HG+ S+AH IN    LRSKLQE  S  + Q+ PLLRLENES+Q C+TF+ NLI
Sbjct: 1621 LMLFDAMHGIGSNAHKINADLLLRSKLQELGSSLESQEAPLLRLENESFQTCMTFLDNLI 1680

Query: 1681 LDKPHSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRE 1740
             D+P  Y EAE+E +L+ LC EVL+FY         +  S S      W +P GSGK++E
Sbjct: 1681 SDQPVGYNEAEIESHLISLCREVLEFY---------INISCSKEQSSRWAVPSGSGKKKE 1740

Query: 1741 LATRAPLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVG 1767
            L  RAPL+VA +Q + N+ E+ F+KNL   FPL+++LISCEHGS EVQ+AL++ML+ S+G
Sbjct: 1741 LTARAPLVVAAIQTLGNMGESLFKKNLPELFPLIATLISCEHGSGEVQVALSDMLQTSMG 1749

BLAST of Cp4.1LG03g17170 vs. TAIR 10
Match: AT4G38200.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 1493.0 bits (3864), Expect = 0.0e+00
Identity = 838/1807 (46.38%), Postives = 1150/1807 (63.64%), Query Frame = 0

Query: 1    MASSE---AASRLSQVITPALEKIVKNASWRKHSKLAHECKSV---IERLASSPKPSSPS 60
            M+SS+    A+R  +VI P+L+KI+KNA+WRKH+ L   CKSV   +E L+ SP PSSP 
Sbjct: 1    MSSSQNLGGATRCGRVIGPSLDKIIKNAAWRKHTFLVSACKSVLDKLEALSDSPDPSSPL 60

Query: 61   PPSSPTDSEAEGAVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKL 120
               + +D++A                       +L PL+ +  +G  K+ +PA+DC  KL
Sbjct: 61   FGLTTSDADA-----------------------VLQPLLLSLDTGYAKVIEPALDCSFKL 120

Query: 121  IAYGYLRGEADPSGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHG 180
             +   LRGE   S      LL KLI ++CK   +G++++EL VL+ LL+AV S  + I G
Sbjct: 121  FSLSLLRGEVCSSS--PDSLLYKLIHAICKVCGIGEESIELAVLRVLLAAVRSPRILIRG 180

Query: 181  DCLLQIVKTCYDIYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELME 240
            DCLL +V+TCY++YL   N  NQ  AK+ L Q+++IVF R EA+S    ++ + V +L+ 
Sbjct: 181  DCLLHLVRTCYNVYLGGFNGTNQICAKSVLAQIMLIVFTRSEANSMDASLKTVNVNDLLA 240

Query: 241  PIEKTDADGSMTQFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADL 300
              +K   +G+     QGFI  +I                          T  E   P D 
Sbjct: 241  ITDKNVNEGNSVHICQGFINDVI--------------------------TAGEAAPPPD- 300

Query: 301  LDSTDKDMLDAKYWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRAL 360
                            ++ +   EG      +G            G+K+R D FL+F+ L
Sbjct: 301  ---------------FALVQPPEEGASSTEDEG-----------TGSKIREDGFLLFKNL 360

Query: 361  CKLSMKTPPKEAMADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCL 420
            CKLSMK   +E   D  L++GK ++LELLK++++N G     D                 
Sbjct: 361  CKLSMKFSSQENTDDQILVRGKTLSLELLKVIIDNGGPIWLSD----------------- 420

Query: 421  YNRFLGAIKQYLCLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLR 480
              RFL AIKQ LCLSLLKNSA ++M IFQL C+IF +L+ ++R+G+K+E+G+FFPM+VLR
Sbjct: 421  -ERFLNAIKQLLCLSLLKNSALSVMSIFQLQCAIFTTLLRKYRSGMKSEVGIFFPMLVLR 480

Query: 481  VLENVAQPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQG 540
            VLENV QP+F QKM VL  +E +C D  +++DIF+N+DCDV S NIFER+VNGLLKTA G
Sbjct: 481  VLENVLQPSFVQKMTVLSLLENICHDPNLIIDIFVNFDCDVESPNIFERIVNGLLKTALG 540

Query: 541  VPPGVATTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSES 600
             PPG +T L P Q++T + E++KCLV+I+K+MG W+++QL + D    K +E    ++  
Sbjct: 541  PPPGSSTILSPVQDITFRHESVKCLVSIIKAMGTWMDQQLSVGDSLLPKSLENEAPANNH 600

Query: 601  GNVPISNGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEF 660
             N    +GTT +H    D H +++ + SD +T+EQRRAYK+E Q+G++LFNRKP KGIEF
Sbjct: 601  SNSNEEDGTTIDH----DFHPDLNPESSDAATLEQRRAYKIERQKGVTLFNRKPSKGIEF 660

Query: 661  LINANKVGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDE 720
            LI++ KVG+SP+E+ +FL++ +GL+ ++IGDYLGERED  +KVMHAYVDSFDF+ + F E
Sbjct: 661  LISSKKVGNSPDEVVSFLRNTTGLNATMIGDYLGEREDFPMKVMHAYVDSFDFKEMNFGE 720

Query: 721  AIRALLKGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNP 780
            AIR  L+GFRLPGEAQKIDRIMEKFAER+CKCNP +F SADTAYVLAYSVI+LNTDAHN 
Sbjct: 721  AIRFFLRGFRLPGEAQKIDRIMEKFAERFCKCNPNSFSSADTAYVLAYSVIMLNTDAHNI 780

Query: 781  MVKNKMSAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNK 840
            MVK KM+  DFIRNNRGIDDGKDLPEEYL +LY+++  NEIKM  D  AP+ RQS   NK
Sbjct: 781  MVKEKMTKADFIRNNRGIDDGKDLPEEYLGALYDQVVINEIKMSSDSSAPESRQSNGLNK 840

Query: 841  LLGFDSILNIVIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIE 900
            LLG D ILN+V   + E++ +  +  LIK +QE+F+ ++ KSES Y+  TDV IL+FM+E
Sbjct: 841  LLGLDGILNLVYWTQTEEKAVGANGLLIKDIQEKFRSKSGKSESAYHVVTDVAILRFMVE 900

Query: 901  VCWAPMLAAFSVPLDRSDDEVIIALCLEGFQYAIHVTA---------------------- 960
            V W PMLAAFSV LD+SDD +    CL GF+YA+HVTA                      
Sbjct: 901  VSWGPMLAAFSVTLDQSDDRLAAVECLRGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLH 960

Query: 961  --------------AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAF 1020
                          AI+ IA E+GN LQ+AWEHILTC+SR EHL LLGEGAP DA++FA 
Sbjct: 961  CAGDMKQKNVDAVKAIISIAIEDGNHLQDAWEHILTCLSRIEHLQLLGEGAPSDASYFA- 1020

Query: 1021 PQNESEKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASG-VTSEQMNNL 1080
                S ++++ K+   P LKKKG  +     A V  GSYDS+ I  N  G V  +Q+NN 
Sbjct: 1021 ----STETEEKKALGFPNLKKKGALQNPVMMAVVRGGSYDSSTIGPNMPGLVKQDQINNF 1080

Query: 1081 VSNLNMLEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIV 1140
            ++NLN+L+Q+GS ++N ++  SQ+L +EAIV FVKALCKVSM EL+S +DPRVFSLTK+V
Sbjct: 1081 IANLNLLDQIGSFQLNNVYAHSQRLKTEAIVAFVKALCKVSMSELQSPTDPRVFSLTKLV 1140

Query: 1141 EIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANY 1200
            EIAHYNMNRIRLVWS IW +LSDFFV++G SENLS+AIF MDSLRQLSMKFL+REELANY
Sbjct: 1141 EIAHYNMNRIRLVWSRIWSILSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANY 1200

Query: 1201 NFQNEFMKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDH 1260
            NFQNEF++PFVIVM+KSS+ EIRELI+RC+SQMVLSRV+NVKSGWKS+F VFTTAA+D+ 
Sbjct: 1201 NFQNEFLRPFVIVMQKSSSAEIRELIVRCISQMVLSRVSNVKSGWKSVFKVFTTAAADER 1260

Query: 1261 KNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFC 1320
            KNIVLLAFE +EKI+R+YF YITETE TTFTDCV CLI FTN+ F  D+SLNAIAFLRFC
Sbjct: 1261 KNIVLLAFETMEKIVREYFSYITETEATTFTDCVRCLITFTNSTFTSDVSLNAIAFLRFC 1320

Query: 1321 ATKLAEGDLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGLSE 1380
            A KLA+G L  + + +        S  +P+            D D ++ +W PLL GLS+
Sbjct: 1321 ALKLADGGLVWNEKGRSS------SPSTPVTDDHSPSTQNFMDADENISYWVPLLTGLSK 1380

Query: 1381 LSFDPRPEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSE 1440
            L+ D R  IRKS+L+VLF+ L+ HGH FS   W  VF SV++PIF+ V    D  S    
Sbjct: 1381 LTSDSRSAIRKSSLEVLFNILKDHGHIFSRTFWIGVFSSVIYPIFNSVWGENDLLSKDEH 1440

Query: 1441 QGADSE-NGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQS 1500
                S  +    + +W  ET  +A Q +VDLFV F++ +   L  V++LL   I+ P Q 
Sbjct: 1441 SSFPSTFSSHPSEVSWDAETSAMAAQYLVDLFVSFFTVIRSQLSSVVSLLAGLIRSPAQG 1500

Query: 1501 LAGIGIAAFVRLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLEL 1560
                G+ A +RL    GD FSE +W+E+  A+ EA S TL  F   + T   I       
Sbjct: 1501 PTVAGVGALLRLADELGDRFSENEWKEIFLAVNEAASLTLSSFMKTLRTMDDIPDE---- 1560

Query: 1561 NGESNAETNGSELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLST 1620
              ++ ++ + S   D D +SL+T    ++ +  K    VQL ++Q V + Y +++  L  
Sbjct: 1561 --DTLSDQDFSNEDDIDEDSLQTMSYVVART--KSHITVQLQVVQVVTDLYRIHQQSLLA 1620

Query: 1621 KNMLILFDALHGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQ 1680
             ++ ++ + L  ++SHAH +N+   L+ K++   SI ++ +PP+L  EN+++Q  L  +Q
Sbjct: 1621 SHVTVILEILSSISSHAHQLNSDLILQKKVRRACSILELSEPPMLHFENDTFQNYLDILQ 1680

Query: 1681 NLILDKPHSYEEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQP-HWPIPLGSG 1740
             ++ + P    E  VE  L+ +C ++L+ Y++     L          QP +W +P+G+ 
Sbjct: 1681 AIVTNNPGVSLELNVESQLMTVCMQILKMYLKCT---LFQGDELEETRQPKNWILPMGAA 1685

Query: 1741 KQRELATRAPLIVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLR 1763
             + E A R+PL+VA+L+A+  L   SF++    FFPLL  L+  EH S++V   L+ +  
Sbjct: 1741 SKEEAAARSPLVVAVLKALRELKRDSFKRYAPNFFPLLVELVRSEHSSSQVPQVLSTVFH 1685

BLAST of Cp4.1LG03g17170 vs. TAIR 10
Match: AT4G35380.1 (SEC7-like guanine nucleotide exchange family protein )

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 819/1796 (45.60%), Postives = 1133/1796 (63.08%), Query Frame = 0

Query: 7    ASRLSQVITPALEKIVKNASWRKHSKLAHECKSVIERLASSPKPSSPSPPSSPTDSEAEG 66
            A+R  ++I P+L+KI+KNA+WRKH+ L   CKSV+++L S   P     PSS     A  
Sbjct: 10   ATRCGRIIGPSLDKIIKNAAWRKHTYLVSSCKSVLDKLES--LPDDFHDPSSVVSGLA-- 69

Query: 67   AVPGPLNDGGPDEHSLAESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYLRGEADP 126
                            ++++S+L P + +  +   K+ +P++DC  KL +   LRGE   
Sbjct: 70   ---------------ASDADSVLQPFLLSLETAYSKVVEPSLDCAFKLFSLSILRGEIQS 129

Query: 127  SGGVEGKLLAKLIESVCKCHDLGDDALELLVLKTLLSAVTSISLRIHGDCLLQIVKTCYD 186
            S   +  +L KL+ +V K   + ++ ++L VL+ LL+AV S  + I GDCLL +VKTCY+
Sbjct: 130  S--KQDSILFKLVNAVSKVGAIAEEPIQLAVLRVLLAAVRSPCILIRGDCLLHVVKTCYN 189

Query: 187  IYLDSKNVINQTTAKASLIQMLVIVFRRMEADSSTVPVQPIVVAELMEPIEKTDADGSMT 246
            IYL   +   Q  AK+ L QM++++F R E DS  V V+ I V EL+   +K+  +GS  
Sbjct: 190  IYLGGLSGTTQICAKSVLAQMMLVIFTRSEEDSLDVSVKTIYVNELLTFTDKSVNEGSSV 249

Query: 247  QFVQGFITKIIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDKDMLDAK 306
             F QGF+ +++    G   P  P  + I   +   ET TV T +                
Sbjct: 250  YFCQGFVNEVMAAGQGSPLP-PPDVIQILLQNP--ETETVMTPDSPSF------------ 309

Query: 307  YWEISMYKTALEGRKGELADGEGERDDDLEIQIGNKLRRDAFLVFRALCKLSMKTPPKEA 366
                          +G +A+GEG    D E    +K+R+DAFL+F+ LCKLSM+   KE 
Sbjct: 310  --------------RGYVANGEG----DSETGDMSKVRQDAFLLFKNLCKLSMRFSSKEN 369

Query: 367  MADPQLMKGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLGAIKQYL 426
              D  +++GK ++LELLK++++N G             W+   +       F+ A+KQYL
Sbjct: 370  NDDQIMVRGKTLSLELLKVIIDNGGSV-----------WRTNES-------FINAVKQYL 429

Query: 427  CLSLLKNSASTLMIIFQLSCSIFISLVSRFRAGLKAEIGVFFPMIVLRVLENVAQPNFQQ 486
            CLSLLKNSA ++M IFQL C+IF+SL+S+ R+ LKAEIG+FFPMIVLRVLENV QP++ Q
Sbjct: 430  CLSLLKNSAVSIMSIFQLQCAIFMSLLSKLRSVLKAEIGIFFPMIVLRVLENVLQPSYLQ 489

Query: 487  KMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQGVPPGVATTLLPP 546
            KM VL  ++K+  D Q++VDIF+NYDCDV SSNI ER+VNGLLKTA G P G +TTL P 
Sbjct: 490  KMTVLNLLDKMSQDPQLMVDIFVNYDCDVESSNILERIVNGLLKTALGPPTGSSTTLSPA 549

Query: 547  QELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEV-AENSSESGNVPISNGTTD 606
            Q+ T + +++KCLV + K+MG+W+++QL++ +    K  +V A   S +  +    GT  
Sbjct: 550  QDSTFRNDSVKCLVNLAKAMGNWMDQQLKVNETVWPKGSQVYASMDSNASQISELEGTIS 609

Query: 607  EHGEGSDSHSEVST-DISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEFLINANKVGSS 666
            +     DS  + S  +  D S +EQRRAYK+ELQ+GISLFNRKP KG+EFLI+  K+GSS
Sbjct: 610  D----CDSQPDTSNPEAYDASMLEQRRAYKIELQKGISLFNRKPSKGVEFLISTKKIGSS 669

Query: 667  PEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDEAIRALLKGFR 726
            PEE+A+FL   +GL+ ++IGDYLGER++L LKVMHAYVDSF+F+  +F EAIR  L+GFR
Sbjct: 670  PEEVASFLMKTAGLNGTVIGDYLGERDELPLKVMHAYVDSFNFEKKDFVEAIRFFLRGFR 729

Query: 727  LPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNPMVKNKMSAED 786
            LPGEAQKIDRIMEKFAE Y KCNP +F SADTAYVLAYSVI+LNTDAHN MVK+KM+  D
Sbjct: 730  LPGEAQKIDRIMEKFAEHYWKCNPGSFTSADTAYVLAYSVIMLNTDAHNNMVKDKMTKAD 789

Query: 787  FIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQLRQSTNSNKLLGFDSILNI 846
            F+RNNRGIDDGKDLPEEYL SLY+R+ + EI+M  D +APQ +Q    NKLLG D ILN+
Sbjct: 790  FVRNNRGIDDGKDLPEEYLGSLYDRVVKEEIRMNSDTLAPQNKQVNGLNKLLGLDGILNL 849

Query: 847  VIRKRWEDQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKFMIEVCWAPMLAAF 906
            V   + +++    +  LI+ +QEQF+ +  KSESVY+  TD+ IL+F++EV W PMLAAF
Sbjct: 850  VSWMQPDEKPHGANGRLIRDIQEQFQAKPEKSESVYHTVTDISILRFILEVSWGPMLAAF 909

Query: 907  SVPLDRSDDEVIIALCLEGFQYAIHVTA-------------------------------- 966
            SV +D+SDD +  +LCL+GF+YA+HVTA                                
Sbjct: 910  SVTIDQSDDRLATSLCLQGFRYAVHVTAVMGMQTQRDAFVTSMAKFTNLHCAADMKQKNV 969

Query: 967  ----AIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATFFAFPQNESEKSKQ 1026
                AI+ IA E+GN L  +WEHILTC+SR EHL LLGE +P +  +   P  ++E    
Sbjct: 970  DAVKAIITIAIEDGNHLHGSWEHILTCLSRIEHLQLLGEVSPSEKRY--VPTKKAEVD-D 1029

Query: 1027 SKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASG-VTSEQMNNLVSNLNMLEQV 1086
             K+   P LKK+G  +     A V  GSYDS  +  +    VT EQ+ + ++NLN+L+Q+
Sbjct: 1030 KKALGFPNLKKRGSFQNPSVMAVVRGGSYDSTSLVKSVPKLVTPEQIKSFIANLNLLDQI 1089

Query: 1087 GSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTKIVEIAHYNMNRI 1146
            G+ E+N ++  SQ+LNSEAIV FVKALCKVSM EL+S +DPRVFSLTK+VE AHYNMNRI
Sbjct: 1090 GNFELNHVYANSQRLNSEAIVSFVKALCKVSMSELQSPTDPRVFSLTKLVETAHYNMNRI 1149

Query: 1147 RLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELANYNFQNEFMKPF 1206
            RLVWS IW+VLSDFFV++G SENLS+AIF MDSLRQLSMKFL+REELANY+FQ+EF++PF
Sbjct: 1150 RLVWSRIWNVLSDFFVSVGLSENLSVAIFVMDSLRQLSMKFLEREELANYHFQHEFLRPF 1209

Query: 1207 VIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASDDHKNIVLLAFEI 1266
            V+VM+KSS+ EIRELI+RCVSQMVLSRV+NVKSGWK++F VFTTAA D+ KNIVLLAFE 
Sbjct: 1210 VVVMQKSSSAEIRELIVRCVSQMVLSRVSNVKSGWKNVFTVFTTAALDERKNIVLLAFET 1269

Query: 1267 IEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLRFCATKLAEGDLG 1326
            IEKI+RD+F  I ETE T + DC+ CLI FTN++F  DI  N I FLRFCA KL EG L 
Sbjct: 1270 IEKIVRDHFHCIIETEITVYADCIRCLITFTNSKFEGDIGFNTIEFLRFCALKLEEGGLV 1329

Query: 1327 SSSRNKDKELSGKISSLSPLRAKDGKHDTE-MTDKDNHLYFWFPLLAGLSELSFDPRPEI 1386
             + + K+  +S           K+   DT+  TD D  + +W PLL GL +   DPRP I
Sbjct: 1330 LNEKLKNNTISA---------LKEDFSDTQSFTDLDEQVSYWIPLLTGLCKQVSDPRPAI 1389

Query: 1387 RKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTSSEQGADSENGE 1446
            RK +++VLF  L  HGH F+ P W  +F S++ P+F+ +R   D     S     S + +
Sbjct: 1390 RKRSIEVLFHILMDHGHLFTRPFWTGIFSSIILPVFNNIRSKTDMLFEESVDSPSSASLD 1449

Query: 1447 LDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQSLAGIGIAAFV 1506
             ++  W  ET TLALQL+VDL VKF+ +V   L  V++++V FIK P Q   G GI+  +
Sbjct: 1450 TEETTWDVETSTLALQLLVDLLVKFFRSVRSQLPSVVSIIVGFIKSPFQGSTGSGISVLL 1509

Query: 1507 RLMSNAGDLFSEEKWQEVVFALGEATSATLPDFTFLVNTDSTIRSHRLELNGESNAETNG 1566
             L        SE++W+E+  AL EA S T   F  ++ T   I      L+G+S    N 
Sbjct: 1510 HLADGLARSASEDEWREIFLALKEAASLTFAGFMKVLRTMDDIEDVE-TLSGQS---VNI 1569

Query: 1567 SELPDDDTESLRTQHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFDAL 1626
             +L DD    +     Y+ +S  K    V   +++ V + Y   +  LS  ++ IL D  
Sbjct: 1570 GDLDDDSLHIMS----YV-VSRTKKHIDVLSQIVEVVSDLYRRNQFSLSASHVDILADIF 1629

Query: 1627 HGVASHAHSINTSSALRSKLQEFASITQMQDPPLLRLENESYQICLTFVQNLILDKPHSY 1686
              +ASHA  +NT + LR K +   S+  + +P LL  ENE+Y+  + F+Q+++   P+  
Sbjct: 1630 SCIASHAQQLNTDTVLRRKFKRACSVQNLTEPQLLNFENEAYKSYMMFLQDMVTCNPNVS 1689

Query: 1687 EEAEVELYLVKLCHEVLQFYVETARYGLVVEASASSGTQPHWPIPLGSGKQRELATRAPL 1746
            +E ++E  LV  C ++++ Y++        +          W +P+ S +  E   R  L
Sbjct: 1690 KELDLESRLVTECAKIVKIYLKCTD---PQQQEQQQRKPVLWVLPMESDRVEEATARTSL 1705

Query: 1747 IVAILQAICNLNEASFEKNLAGFFPLLSSLISCEHGSNEVQLALTEMLRASVGPIL 1763
            +V+ L+A+C+L   S +K+++ FFPLL  L+  EH S +V   L+ +L++ +GPIL
Sbjct: 1750 LVSSLEALCSLEAESLKKHVSSFFPLLVDLVRTEHCSPQVPYVLSNVLKSCIGPIL 1705

BLAST of Cp4.1LG03g17170 vs. TAIR 10
Match: AT3G43300.1 (HOPM interactor 7 )

HSP 1 Score: 827.8 bits (2137), Expect = 1.7e-239
Identity = 635/1851 (34.31%), Postives = 902/1851 (48.73%), Query Frame = 0

Query: 83   AESESILSPLINASSSGVLKIADPAVDCIQKLIAYGYLRGEADPSGGVEGKLLAKLIESV 142
            AE E +L PL  A  +  LKI D A+DC+ KLIAY +L G+    GG        ++  V
Sbjct: 102  AEVELVLKPLRLAFETKNLKIFDAALDCLHKLIAYDHLEGDPGLDGGKNSAPFTDILNMV 161

Query: 143  CKCHD-LGDDALELLVLKTLLSAVTSISLRIHGDCLLQIVKTCYDIYLDSKNVINQTTAK 202
            C C D    D+  L VLK LL+AV S   ++HG+ LL +++ CY+I L+SK+ INQ T+K
Sbjct: 162  CSCVDNSSPDSTVLQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPINQATSK 221

Query: 203  ASLIQMLVIVFRRMEAD----SSTVPVQPIVVAELMEP--IEKTDADGSMTQFVQGFITK 262
            A L QM+ IVFRRME D    SSTV  +  V  +   P   E T AD +  +   G    
Sbjct: 222  AMLTQMISIVFRRMETDIVSASSTVSQEEHVSGDTSSPKNEEITAADENEKEMTLG---- 281

Query: 263  IIQDIDGVLNPATPGKVSIGAHDGAFETTTVETTNPADLLDSTDKDMLDAKYWEISMYKT 322
                                A   A +TT         L+   D   L+A          
Sbjct: 282  -------------------DALTQAKDTTLASVEELHTLVGGADIKGLEA---------- 341

Query: 323  ALEGRKGELADGEG-ERDDDLE-IQIGNKLRRDAFLVFRALCKLSMKTPPKEAMADPQLM 382
            AL+ +   L DG+  +R  +LE + IG   +RDA LVFR LCK+ MK       +D    
Sbjct: 342  ALD-KAVHLEDGKKIKRGIELESMSIG---QRDALLVFRTLCKMGMKED-----SDEVTT 401

Query: 383  KGKIVALELLKILLENAGKKCALDFLLLYFWWQIISTKKCLYNRFLGAIKQYLCLSLLKN 442
            K +I++LELL+ +LE        +F                   F+ ++K YL  +LL+ 
Sbjct: 402  KTRILSLELLQGMLEGVSHSFTKNF------------------HFIDSVKAYLSYALLRA 461

Query: 443  SASTLMIIFQLSCSIFISLVSRFRAGLK-------------------AEIGVFFPMIVLR 502
            S S   +IFQ +  IF  L+ RFR  LK                    EIG+FFP+IVLR
Sbjct: 462  SVSQSSVIFQYASGIFSVLLLRFRDSLKVSMDCYLSPYFSDPKSHSQGEIGIFFPIIVLR 521

Query: 503  VLENVAQPNFQQKMIVLRFVEKLCVDSQILVDIFINYDCDVNSSNIFERMVNGLLKTAQG 562
             L+N   PN  QKM VLR +EK+C D Q+LVD+++NYDCD+ + N+FERMV  L K AQG
Sbjct: 522  SLDNSECPN-DQKMGVLRMLEKVCKDPQMLVDVYVNYDCDLEAPNLFERMVTTLSKIAQG 581

Query: 563  VPPGVATTLLPPQELTMKLEAMKCLVAILKSMGDWLNRQLRIPDPHSTKKIEVAENSSES 622
                     +  Q  ++K  +++CLV +LKS+ DW             K    AENS+ +
Sbjct: 582  SQSADPNPAMASQTASVKGSSLQCLVNVLKSLVDW------------EKIRREAENSTRN 641

Query: 623  GNVPISNGTTDEHGEGSDSHSEVSTDISDVSTIEQRRAYKLELQEGISLFNRKPKKGIEF 682
             N        D    G    ++   D+   S  E+ +A+K  ++  IS FNR   KG+E+
Sbjct: 642  AN-------EDSASTGEPIETKSREDVP--SNFEKAKAHKSTMEAAISEFNRNSVKGVEY 701

Query: 683  LINANKVGSSPEEIAAFLKDASGLDKSLIGDYLGEREDLSLKVMHAYVDSFDFQGLEFDE 742
            LI    V  +P  +A FL+  S L K +IGDYLG+ E+  L VMHAYVDS  F  ++F  
Sbjct: 702  LIANKLVERNPASVAQFLRSTSSLSKVMIGDYLGQHEEFPLAVMHAYVDSMKFSEMKFHS 761

Query: 743  AIRALLKGFRLPGEAQKIDRIMEKFAERYCKCNPKAFISADTAYVLAYSVILLNTDAHNP 802
            AIR  LKGFRLPGEAQKIDRIMEKFAERYC  NP  F +ADTAYVLAY+VI+LNTDAHNP
Sbjct: 762  AIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNP 821

Query: 803  MVKNKMSAEDFIRNNRGIDDGKDLPEEYLKSLYERISRNEIKMKDDEMAPQL-RQSTNSN 862
            MV  KMS  DF R N   D     P E L+ +Y+ I + EIK+KDD+   +L  Q     
Sbjct: 822  MVWPKMSKSDFTRMNATNDPEDCAPTELLEEIYDSIVQEEIKLKDDDTMKKLSSQRPGGE 881

Query: 863  KLLGFDSILNIVIRKRWE--DQNMETSDDLIKHMQEQFKERARKSESVYYAATDVVILKF 922
            +  G  SILN+ + KR    D   ET +D+++  QE F++   K   V++    V I++ 
Sbjct: 882  ERGGLVSILNLGLPKRISAADAKSET-EDIVRKTQEIFRKHGVK-RGVFHTVEQVDIIRP 941

Query: 923  MIEVCWAPMLAAFSVPLDRSDDEVIIALCLEGFQYAIHVT-------------------- 982
            M+E    P+LAAFSV ++  D++  I LC+EGF+  IH+                     
Sbjct: 942  MVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFKAGIHIAYVLGMDTMRYAFLTSLVRFT 1001

Query: 983  ----------------AAIVKIADEEGNFLQEAWEHILTCVSRFEHLHLLGEGAPPDATF 1042
                              ++ + D E + LQ+ W  +L CVSR E               
Sbjct: 1002 FLHAPKEMRSKNVEALRILLGLCDSEPDTLQDTWNAVLECVSRLEF-------------- 1061

Query: 1043 FAFPQNESEKSKQSKSTMLPLLKKKGVGRIQYAAAAVMRGSYDSAGITGNASGVTSEQMN 1102
                                ++   G+      AA VM GS        +  GV      
Sbjct: 1062 --------------------IISTPGI------AATVMHGSNQI-----SRDGV------ 1121

Query: 1103 NLVSNLNMLEQVGSSEMNRIFTRSQKLNSEAIVDFVKALCKVSMEELRSTSDPRVFSLTK 1162
                 +  L+++      ++F  S KL SE++V+F  ALC VS EEL+  S  RVFSL K
Sbjct: 1122 -----VQSLKELAGRPAEQVFVNSVKLPSESVVEFFTALCGVSAEELKQ-SPARVFSLQK 1181

Query: 1163 IVEIAHYNMNRIRLVWSSIWHVLSDFFVTIGCSENLSIAIFAMDSLRQLSMKFLDREELA 1222
            +VEI++YN+ RIR+VW+ IW VL++ FV+ G   +  IA++A+DSLRQL MK+L+R EL 
Sbjct: 1182 LVEISYYNIARIRMVWARIWSVLAEHFVSAGSHHDEKIAMYAIDSLRQLGMKYLERAELT 1241

Query: 1223 NYNFQNEFMKPFVIVMRKSSAVEIRELIIRCVSQMVLSRVNNVKSGWKSMFMVFTTAASD 1282
            N+ FQN+ +KPFVI+MR + +  IR LI+ C+ QM+ S+V ++KSGW+S+FM+FT AA D
Sbjct: 1242 NFTFQNDILKPFVIIMRNTQSQTIRSLIVDCIVQMIKSKVGSIKSGWRSVFMIFTAAADD 1301

Query: 1283 DHKNIVLLAFEIIEKIIRDYFPYITETETTTFTDCVNCLIAFTNNRFNKDISLNAIAFLR 1342
            + ++IV  +FE +E++I ++F  +       F DCVNCLI F NN+ +  ISL AIA LR
Sbjct: 1302 EVESIVEKSFENVEQVILEHFDQVI---GDCFMDCVNCLIRFANNKASDRISLKAIALLR 1361

Query: 1343 FCATKLAEGDLGSSSRNKDKELSGKISSLSPLRAKDGKHDTEMTDKDNHLYFWFPLLAGL 1402
             C  +LAEG +                    L+  DG  D E  D   H  +WFP+LAGL
Sbjct: 1362 ICEDRLAEGLIPG----------------GVLKPVDGNED-ETFDVTEH--YWFPMLAGL 1421

Query: 1403 SELSFDPRPEIRKSALQVLFDTLRKHGHHFSLPLWERVFESVLFPIFDYVRHAIDPSSTS 1462
            S+L+ D RPE+R  AL+VLFD L + G+ FS P WE +F  +LFPIFD+V HA   S  S
Sbjct: 1422 SDLTSDYRPEVRNCALEVLFDLLNERGNKFSTPFWESIFHRILFPIFDHVSHAGKESLIS 1481

Query: 1463 SEQGADSENGELDQDAWLYETCTLALQLVVDLFVKFYSTVNPLLKKVLTLLVSFIKRPHQ 1522
            S             D    ET   +LQL+ +LF  FY  V  +L  +L+LL+   K+  Q
Sbjct: 1482 S------------GDVKFRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKKSDQ 1541

Query: 1523 SLAGIGIAAFVRLMSNAGDLFSEEKWQEVVFALGEATSATLP------------------ 1582
            ++  I + A V L+   G  FSE  W  ++ ++ +A+  T P                  
Sbjct: 1542 TVVSISLGALVHLIEVGGHQFSEGDWDMLLKSIRDASYTTQPLELLNALSFDNPKKNLVL 1601

Query: 1583 -----------------------DFTFLVNTDSTIRSHRLELNGESNAETNGSE------ 1642
                                   +          I +H   L      + +GSE      
Sbjct: 1602 AGDIEADASDSPRVDRNPDDIKDNGKVSAQASPRIGTHGTSLESGIPPKADGSEGRPSSS 1661

Query: 1643 -------------------------------------------LP------DDDTESLRT 1702
                                                       +P      +D TE    
Sbjct: 1662 GRAQKDVDDVNLQRSQTFGQRFMDNLFLRNLTSQPKSSVAEVTVPSSPYKHEDPTEPDSR 1721

Query: 1703 QHVYISMSDAKCRAAVQLLLIQAVMETYNMYRSHLSTKNMLILFDALHGVASHAHSINTS 1762
            +    ++   + +   QLLL+ A+      Y S+L T   + + D L      A S N+ 
Sbjct: 1722 EEESPALGAIRGKCITQLLLLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFASSYNSY 1756