Cp4.1LG03g16900 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g16900 vs. ExPASy Swiss-Prot
Match: Q50EK3 (Cytochrome P450 704C1 OS=Pinus taeda OX=3352 GN=CYP704C1 PE=2 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 6.8e-130
Identity = 242/509 (47.54%), Postives = 357/509 (70.14%), Query Frame = 0

Query: 18  ISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLARKHKTYRL 77
           ++V+AL L  S+ +   L    +K  Y P+ GT+ +  +NF RLHDY TD A+++KT+R+
Sbjct: 10  VTVSALALACSLWIASYLRNWRKKGVYPPVVGTMLNHAINFERLHDYHTDQAQRYKTFRV 69

Query: 78  LGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQRKIS 137
           + P  + ++T+DP NVE++L+TNF N+ KG+ N+ I++DLLGDGIF VDG+KWRQQRK++
Sbjct: 70  VYPTCSYVFTTDPVNVEHILKTNFANYDKGTFNYDIMKDLLGDGIFNVDGDKWRQQRKLA 129

Query: 138 SYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVSFGID 197
           S EF+++VL+++SS +F   A KLA I+++A+    ++++QDLFM+S+LDSI  V FGID
Sbjct: 130 SSEFASKVLKDFSSGVFCNNAAKLANILAQAAKLNLSVEMQDLFMRSSLDSICKVVFGID 189

Query: 198 LDSLHGVNEETG---KFCNAFEASSTMTLWRY-VDVAWPLKKILNIGSEATLKENIKIVD 257
           ++SL     E+G    F  AF+ ++ M   R+ V   W +++  N+GSEA L++NIK+VD
Sbjct: 190 INSLSSSKAESGPEASFAKAFDVANAMVFHRHMVGSFWKVQRFFNVGSEAILRDNIKMVD 249

Query: 258 EYIYKLIHSALEQRQKLGDKTSMRRENIVSRFL---QAET---ADPKYLRDIILNFVIAG 317
           +++YK+IH    +++    +    R +I+SR++     ET      KYLRD+ILNF++A 
Sbjct: 250 DFLYKVIH--FRRQEMFSAEKENVRPDILSRYIIISDKETDGKVSDKYLRDVILNFMVAA 309

Query: 318 KDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVE------DITDFAEFAARLTEESLE 377
           +DTTA+ L+WFIYMLCKH  VQE++ +EI   T+V       +  D A FA  LT+E+L 
Sbjct: 310 RDTTAIALSWFIYMLCKHQHVQEKLLEEIISSTSVHEDQYSTECNDIASFAQSLTDEALG 369

Query: 378 KMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGD 437
           KM +L A+++ETLRLYPA+PV      ++DTLPDGF V+KGD V++ PYAMGRM ++WGD
Sbjct: 370 KMHYLHASLSETLRLYPALPVDGKYVVNEDTLPDGFKVKKGDSVNFLPYAMGRMSYLWGD 429

Query: 438 DAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKM 497
           DA+EF+PERW+  DG   P+SPFKF AFQAGPR C+GK+FAY Q+KI+ +VLV  FKF+ 
Sbjct: 430 DAKEFKPERWIQ-DGIFHPKSPFKFPAFQAGPRTCLGKDFAYLQMKIVAAVLVRFFKFEA 489

Query: 498 SDEKKIVKYQPMINL-LIKGGLQVCAIPR 507
              K+ V+Y+ M+ L + + GL V   PR
Sbjct: 490 VKTKE-VRYRTMLTLHMNEDGLNVQVTPR 514

BLAST of Cp4.1LG03g16900 vs. ExPASy Swiss-Prot
Match: Q9C788 (Cytochrome P450 704B1 OS=Arabidopsis thaliana OX=3702 GN=CYP704B1 PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.2e-103
Identity = 216/524 (41.22%), Postives = 313/524 (59.73%), Query Frame = 0

Query: 23  LLLLISIVVWQILTRKLRKK----KYHPIAGTVFHQLLNFHRLHDYMTDLARKHKTYRLL 82
           L++   +  W  L R  ++     K  P+ G    QL NF R+HD++ +     +T  + 
Sbjct: 5   LVIACMVTSWIFLHRWGQRNKSGPKTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVP 64

Query: 83  GPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQRKISS 142
            PF    Y +DP NVEY+L+TNF N+ KG   H  +  LLGDGIF  DGE WR+QRK +S
Sbjct: 65  MPFTTYTYIADPINVEYVLKTNFSNYPKGETYHSYMEVLLGDGIFNSDGELWRKQRKTAS 124

Query: 143 YEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVSFGIDL 202
           +EF+++ LR++S+V+F+ Y++KL  I+S+AS   Q +D+Q+L M+ TLDSI  V FG+++
Sbjct: 125 FEFASKNLRDFSTVVFKEYSLKLFTILSQASFKEQQVDMQELLMRMTLDSICKVGFGVEI 184

Query: 203 DSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKIVDEYIY- 262
            +L     E   F  AF+ ++ +   R++D  W +KK LNIGSEA L ++IK+V+++ Y 
Sbjct: 185 GTLAPELPE-NHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYS 244

Query: 263 -------KLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADP------KYLRDIILNFV 322
                  +L+ + +       +  +  + +I+SRF++  + DP      K LRDI+LNFV
Sbjct: 245 VIRRRKAELLEAQISPTNNNNNNNNKVKHDILSRFIEI-SDDPDSKETEKSLRDIVLNFV 304

Query: 323 IAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVT--------------NVEDITDF-- 382
           IAG+DTTA TL W IYM+  +  V E++  E++E+               + ED   F  
Sbjct: 305 IAGRDTTATTLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFNE 364

Query: 383 --AEFAARLTEESLEKMQFLTATINETLRLYPAVPVI---CFSDDTLPDGFSVRKGDMVS 442
              EFA  L  +SL K+ +L A I ETLRLYPAVP        DD LP+G  V+ G MV+
Sbjct: 365 KVTEFAGLLNYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVT 424

Query: 443 YQPYAMGRMKFIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQI 502
           Y PY+MGRM++ WG DA  F+PERWL  DG  Q  SPFKFTAFQAGPRIC+GK+ AY Q+
Sbjct: 425 YVPYSMGRMEYNWGSDAALFKPERWLK-DGVFQNASPFKFTAFQAGPRICLGKDSAYLQM 484

Query: 503 KIMLSVLVGSFKFKMSDEKKIVKYQPMINLLIKGGLQVCAIPRS 508
           K+ +++L   +KF +      VKY+ M  L +  GL+V    RS
Sbjct: 485 KMAMAILCRFYKFHLVPNHP-VKYRMMTILSMAHGLKVTVSRRS 524

BLAST of Cp4.1LG03g16900 vs. ExPASy Swiss-Prot
Match: Q9FMY1 (Cytochrome P450 86B1 OS=Arabidopsis thaliana OX=3702 GN=CYP86B1 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 4.9e-80
Identity = 187/512 (36.52%), Postives = 298/512 (58.20%), Query Frame = 0

Query: 20  VAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNF------HRLHDYMTD-LARKH 79
           V  L LLI+I V+ +    LR+KKY  +    F  +L          ++++++D L  ++
Sbjct: 36  VQILELLIAIFVF-VAIHALRQKKYQGLPVWPFLGMLPSLAFGLRGNIYEWLSDVLCLQN 95

Query: 80  KTYRLLGPF---RNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEK 139
            T++  GP+    N   T DP NVE++L+  F  F KGS+    LRDLLGDGIF  D E 
Sbjct: 96  GTFQFRGPWFSSLNSTITCDPRNVEHLLKNRFSVFPKGSYFRDNLRDLLGDGIFNADDET 155

Query: 140 WRQQRKISSYEFSTRVLREYSS-VIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDS 199
           W++QRK +S EF +   R+ ++  +F     +L  ++  +  S   ID+QD+ ++ T D+
Sbjct: 156 WQRQRKTASIEFHSAKFRQLTTQSLFELVHKRLLPVLETSVKSSSPIDLQDVLLRLTFDN 215

Query: 200 IFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYV--DVAWPLKKILNIGSEATLK 259
           +  ++FG+D   L G ++    F  AFE ++   + R+V     W   + L+IG+E  LK
Sbjct: 216 VCMIAFGVDPGCL-GPDQPVIPFAKAFEDATEAAVVRFVMPTCVWKFMRYLDIGTEKKLK 275

Query: 260 ENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFL-----QAETADPKYLRDIIL 319
           E+IK VD++  ++I +  ++    G+ T  +R ++++ F+     + E+   K+LRDI +
Sbjct: 276 ESIKGVDDFADEVIRTRKKELSLEGETT--KRSDLLTVFMGLRDEKGESFSDKFLRDICV 335

Query: 320 NFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAE---FAARLTEE 379
           NF++AG+DT++V L+WF ++L K+P V+E++  E+ ++    D    AE   +      E
Sbjct: 336 NFILAGRDTSSVALSWFFWLLEKNPEVEEKIMVEMCKILRQRDDHGNAEKSDYEPVFGPE 395

Query: 380 SLEKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFI 439
            ++KM +L A ++E LRLYP+VPV       DD  PDG  ++KGD V Y  YAMGRM+ I
Sbjct: 396 EIKKMDYLQAALSEALRLYPSVPVDHKEVQEDDVFPDGTMLKKGDKVIYAIYAMGRMEAI 455

Query: 440 WGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFK 499
           WG D  EFRPERWL  DG    +S +KFTAF  GPR+C+GK+FAY Q+K   + +V  +K
Sbjct: 456 WGKDCLEFRPERWL-RDGRFMSESAYKFTAFNGGPRLCLGKDFAYYQMKSTAAAIVYRYK 515

Query: 500 FKMSDEKKIVKYQPMINLLIKGGLQVCAIPRS 508
            K+ +  K V+ +  + + +K GL V  I RS
Sbjct: 516 VKVVNGHK-VEPKLALTMYMKHGLMVNLINRS 541

BLAST of Cp4.1LG03g16900 vs. ExPASy Swiss-Prot
Match: Q9CAD6 (Cytochrome P450 86A7 OS=Arabidopsis thaliana OX=3702 GN=CYP86A7 PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 3.9e-77
Identity = 172/503 (34.19%), Postives = 297/503 (59.05%), Query Frame = 0

Query: 23  LLLLISIVVWQI-LTRKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLAR----KHKTYRL 82
           L L+++ ++W + L R  +  +  P+ G++   + N HR+HD++ D  R     ++T   
Sbjct: 10  LTLIVTYIIWFVSLRRSYKGPRVWPLVGSLPALITNAHRMHDFIADNLRMCGGTYQTCIF 69

Query: 83  LGPF-----RNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQ 142
             PF      +   T DP N+E++L+T F+N+ KG     +  DLLGDGIF  DG+ WR 
Sbjct: 70  PIPFLAKKQGHVTVTCDPKNLEHILKTRFDNYPKGPSWQSVFHDLLGDGIFNSDGDTWRF 129

Query: 143 QRKISSYEFSTRVLRE-YSSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFH 202
           QRK ++ EF+TR LR+  +  + R    +L  I+  A +  + ID+QD+ ++ T D+I  
Sbjct: 130 QRKTAALEFTTRTLRQAMARWVDRAIKNRLVPILESARSRAEPIDLQDVLLRLTFDNICG 189

Query: 203 VSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYV--DVAWPLKKILNIGSEATLKENI 262
           ++FG D  +L     E G F  AF+ ++  TL R++  +  W ++K L +G E  +  +I
Sbjct: 190 LTFGKDPRTLSPEFPENG-FAVAFDGATEATLQRFIMPEFIWKIRKWLRLGLEDDMSRSI 249

Query: 263 KIVDEYIYKLIHSALEQRQKLG-DKTSMRRENIVSRFL-QAETADPKYLRDIILNFVIAG 322
             VD Y+ ++I++   + + LG  +   R ++++SRF+ + E+   KYL+ + LNF++AG
Sbjct: 250 SHVDNYLSEIINT--RKLELLGQQQDESRHDDLLSRFMKKKESYSDKYLKYVALNFILAG 309

Query: 323 KDTTAVTLAWFIYMLCKHPAVQERVAKEIKEV------TNVEDITDFAEFAARLTEESLE 382
           +DT++V ++WF +++  +P V+E++  EI  +      TNV   TD       LT + ++
Sbjct: 310 RDTSSVAMSWFFWLVSLNPRVEEKIINEICTILIKTRDTNVSKWTD-----EPLTFDEID 369

Query: 383 KMQFLTATINETLRLYPAVP---VICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGD 442
           ++ +L A ++ETLRLYP+VP       ++D LPDG  V  G  V+Y  Y++GRMKFIWG+
Sbjct: 370 QLVYLKAALSETLRLYPSVPEDSKFVVANDVLPDGTFVPSGSNVTYSIYSVGRMKFIWGE 429

Query: 443 DAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKM 502
           D  EF+PERWL+ +   +  + +KF AF AGPRIC+GK+ AY Q+K + + ++   +  +
Sbjct: 430 DCLEFKPERWLE-ESRDEKCNQYKFVAFNAGPRICLGKDLAYLQMKSITASILLRHRLTV 489

BLAST of Cp4.1LG03g16900 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 288.9 bits (738), Expect = 1.1e-76
Identity = 158/477 (33.12%), Postives = 280/477 (58.70%), Query Frame = 0

Query: 43  KYHPIAGTVFHQLLNFHRLHDYMTDLAR--KHKTYRLLGPF-RNEIYTSDPPNVEYMLRT 102
           K +P+ G+      N HR   +++D+ +     T++L G   + +I T +P  V+++L+ 
Sbjct: 49  KSYPLIGSYLSFRKNLHRRIQWLSDIVQISPSATFQLDGTLGKRQIITGNPSTVQHILKN 108

Query: 103 NFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQRKISSYEFSTRVLREY-SSVIFRRYA 162
            F N+ KG+     L D LG GIF  +G  W+ QR+++S+EF+T+ +R +   ++     
Sbjct: 109 QFSNYQKGTTFTNTLSDFLGTGIFNTNGPNWKFQRQVASHEFNTKSIRNFVEHIVDTELT 168

Query: 163 VKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEAS 222
            +L  I++ ++ +   +D QD+  + T D+I +++FG D + L   +    KF  A+E +
Sbjct: 169 NRLIPILTSSTQTNNILDFQDILQRFTFDNICNIAFGYDPEYL-TPSTNRSKFAEAYEDA 228

Query: 223 STMTLWRY---VDVAWPLKKILNIGSEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTS 282
           + ++  R+   + + W +KK  NIGSE  LKE +  V  +  KL+    E++++L +K+S
Sbjct: 229 TEISSKRFRLPLPIIWKIKKYFNIGSEKRLKEAVTEVRSFAKKLVR---EKKRELEEKSS 288

Query: 283 MRRENIVSRFLQAETADPKYLRDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKE 342
           +  E+++SRFL +  +D  ++ DI+++F++AGKDTT+  L WF ++L K+P V+E +  E
Sbjct: 289 LETEDMLSRFLSSGHSDEDFVADIVISFILAGKDTTSAALTWFFWLLWKNPRVEEEIVNE 348

Query: 343 IKEVTNVEDITDFAEFAARLTEESLEKMQFLTATINETLRLYPAVPV---ICFSDDTLPD 402
           + + + +            +  + +++M +  A ++E++RLYP VP+      +DD LPD
Sbjct: 349 LSKKSEL------------MVYDEVKEMVYTHAALSESMRLYPPVPMDSKEAVNDDVLPD 408

Query: 403 GFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFRPERWLD-----GDGNVQPQSPFKFTAFQ 462
           G+ V+KG +V+Y  YAMGRMK +WGDD  EFRPERWL+     G      +  + +  FQ
Sbjct: 409 GWVVKKGTIVTYHVYAMGRMKSLWGDDWAEFRPERWLEKDEVNGKWVFVGRDSYSYPVFQ 468

Query: 463 AGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEKKIVKYQPMINLL---IKGGLQV 502
           AGPR+C+GKE A+ Q+K +++ +VG  KFK+  E  + +    I+ L   ++GG  V
Sbjct: 469 AGPRVCLGKEMAFMQMKRIVAGIVG--KFKVVPEAHLAQEPGFISFLSSQMEGGFPV 507

BLAST of Cp4.1LG03g16900 vs. NCBI nr
Match: XP_023526604.1 (cytochrome P450 704C1-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1018 bits (2631), Expect = 0.0
Identity = 513/516 (99.42%), Postives = 513/516 (99.42%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII 300
           SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII
Sbjct: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII 300

Query: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL 360
           LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL
Sbjct: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL 360

Query: 361 EKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420
           EKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG
Sbjct: 361 EKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420

Query: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480
           DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK
Sbjct: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480

Query: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS
Sbjct: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 516

BLAST of Cp4.1LG03g16900 vs. NCBI nr
Match: KAG6582575.1 (Cytochrome P450 704C1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018959.1 Cytochrome P450, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 998 bits (2581), Expect = 0.0
Identity = 504/516 (97.67%), Postives = 509/516 (98.64%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVV QILTRKLRKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVRQILTRKLRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRR AVKLAKIVSKASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHV FGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVLFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII 300
           SEATLKENIKIVDEYIYKLIH+ALEQRQKLGDKTSMRRENIVSRFLQAET DPKYLRDII
Sbjct: 241 SEATLKENIKIVDEYIYKLIHNALEQRQKLGDKTSMRRENIVSRFLQAETTDPKYLRDII 300

Query: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL 360
           LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEI+EVTNVED+TDFAEFAARLTEESL
Sbjct: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLTEESL 360

Query: 361 EKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420
           EKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG
Sbjct: 361 EKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420

Query: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480
           DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK
Sbjct: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480

Query: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           MSDE+KIVKYQPMINLLIKGGLQVCAIPRSP+HFNS
Sbjct: 481 MSDEEKIVKYQPMINLLIKGGLQVCAIPRSPDHFNS 516

BLAST of Cp4.1LG03g16900 vs. NCBI nr
Match: XP_022924418.1 (cytochrome P450 704C1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 997 bits (2577), Expect = 0.0
Identity = 503/516 (97.48%), Postives = 508/516 (98.45%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVV QIL RKLRKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVRQILARKLRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRR AVKLAKIVSKASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII 300
           SEATLKENIKIVDEYIYKLIH+ALEQRQKLGDKTSMRRENIVSRFLQAET DPKYLRDII
Sbjct: 241 SEATLKENIKIVDEYIYKLIHNALEQRQKLGDKTSMRRENIVSRFLQAETTDPKYLRDII 300

Query: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL 360
           LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEI+EVTNVED+TDFAEFAARLTEESL
Sbjct: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLTEESL 360

Query: 361 EKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420
           EKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG
Sbjct: 361 EKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420

Query: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480
           DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK
Sbjct: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480

Query: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           MSDE+KIVKYQPMINLLIKGGLQVCA PRSP+HFNS
Sbjct: 481 MSDEEKIVKYQPMINLLIKGGLQVCATPRSPDHFNS 516

BLAST of Cp4.1LG03g16900 vs. NCBI nr
Match: XP_022924417.1 (cytochrome P450 704C1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 991 bits (2562), Expect = 0.0
Identity = 503/520 (96.73%), Postives = 508/520 (97.69%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVV QIL RKLRKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVRQILARKLRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRR AVKLAKIVSKASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTS----MRRENIVSRFLQAETADPKYL 300
           SEATLKENIKIVDEYIYKLIH+ALEQRQKLGDKTS    MRRENIVSRFLQAET DPKYL
Sbjct: 241 SEATLKENIKIVDEYIYKLIHNALEQRQKLGDKTSVDEFMRRENIVSRFLQAETTDPKYL 300

Query: 301 RDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLT 360
           RDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEI+EVTNVED+TDFAEFAARLT
Sbjct: 301 RDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLT 360

Query: 361 EESLEKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMK 420
           EESLEKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMK
Sbjct: 361 EESLEKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMK 420

Query: 421 FIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGS 480
           FIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGS
Sbjct: 421 FIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGS 480

Query: 481 FKFKMSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           FKFKMSDE+KIVKYQPMINLLIKGGLQVCA PRSP+HFNS
Sbjct: 481 FKFKMSDEEKIVKYQPMINLLIKGGLQVCATPRSPDHFNS 520

BLAST of Cp4.1LG03g16900 vs. NCBI nr
Match: XP_022980270.1 (cytochrome P450 704C1 [Cucurbita maxima])

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 501/516 (97.09%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVV QILTRK RKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVRQILTRKQRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLG FRNEIYTSDPPNVEY+LRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGLFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRR AVKLAKIVS+ASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSEASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII 300
           SEATLKENIKIVDEYIYKLIH+ LEQRQKL DKTSMRRENIVSRFLQAET DPKYLRDII
Sbjct: 241 SEATLKENIKIVDEYIYKLIHNTLEQRQKLCDKTSMRRENIVSRFLQAETTDPKYLRDII 300

Query: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL 360
           LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVED+TDFA+FAARLTEESL
Sbjct: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDVTDFAQFAARLTEESL 360

Query: 361 EKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420
           EKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG
Sbjct: 361 EKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420

Query: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480
           DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK
Sbjct: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480

Query: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS
Sbjct: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 516

BLAST of Cp4.1LG03g16900 vs. ExPASy TrEMBL
Match: A0A6J1ECD4 (cytochrome P450 704C1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431924 PE=3 SV=1)

HSP 1 Score: 997 bits (2577), Expect = 0.0
Identity = 503/516 (97.48%), Postives = 508/516 (98.45%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVV QIL RKLRKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVRQILARKLRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRR AVKLAKIVSKASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII 300
           SEATLKENIKIVDEYIYKLIH+ALEQRQKLGDKTSMRRENIVSRFLQAET DPKYLRDII
Sbjct: 241 SEATLKENIKIVDEYIYKLIHNALEQRQKLGDKTSMRRENIVSRFLQAETTDPKYLRDII 300

Query: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL 360
           LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEI+EVTNVED+TDFAEFAARLTEESL
Sbjct: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLTEESL 360

Query: 361 EKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420
           EKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG
Sbjct: 361 EKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420

Query: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480
           DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK
Sbjct: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480

Query: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           MSDE+KIVKYQPMINLLIKGGLQVCA PRSP+HFNS
Sbjct: 481 MSDEEKIVKYQPMINLLIKGGLQVCATPRSPDHFNS 516

BLAST of Cp4.1LG03g16900 vs. ExPASy TrEMBL
Match: A0A6J1E8W4 (cytochrome P450 704C1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431924 PE=3 SV=1)

HSP 1 Score: 991 bits (2562), Expect = 0.0
Identity = 503/520 (96.73%), Postives = 508/520 (97.69%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVV QIL RKLRKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVRQILARKLRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRR AVKLAKIVSKASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSKASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTS----MRRENIVSRFLQAETADPKYL 300
           SEATLKENIKIVDEYIYKLIH+ALEQRQKLGDKTS    MRRENIVSRFLQAET DPKYL
Sbjct: 241 SEATLKENIKIVDEYIYKLIHNALEQRQKLGDKTSVDEFMRRENIVSRFLQAETTDPKYL 300

Query: 301 RDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLT 360
           RDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEI+EVTNVED+TDFAEFAARLT
Sbjct: 301 RDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIEEVTNVEDVTDFAEFAARLT 360

Query: 361 EESLEKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMK 420
           EESLEKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMK
Sbjct: 361 EESLEKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMK 420

Query: 421 FIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGS 480
           FIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGS
Sbjct: 421 FIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGS 480

Query: 481 FKFKMSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           FKFKMSDE+KIVKYQPMINLLIKGGLQVCA PRSP+HFNS
Sbjct: 481 FKFKMSDEEKIVKYQPMINLLIKGGLQVCATPRSPDHFNS 520

BLAST of Cp4.1LG03g16900 vs. ExPASy TrEMBL
Match: A0A6J1IVT5 (cytochrome P450 704C1 OS=Cucurbita maxima OX=3661 GN=LOC111479691 PE=3 SV=1)

HSP 1 Score: 990 bits (2560), Expect = 0.0
Identity = 501/516 (97.09%), Postives = 506/516 (98.06%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHR 60
           MICSVATMVAAKFLSNPISVAALLLLISIVV QILTRK RKKKYHPIAGTVFHQLLNFHR
Sbjct: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVRQILTRKQRKKKYHPIAGTVFHQLLNFHR 60

Query: 61  LHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGD 120
           LHDYMTDLARKHKTYRLLG FRNEIYTSDPPNVEY+LRTNFENFGKGSHNHRILRDLLGD
Sbjct: 61  LHDYMTDLARKHKTYRLLGLFRNEIYTSDPPNVEYILRTNFENFGKGSHNHRILRDLLGD 120

Query: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDL 180
           GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRR AVKLAKIVS+ASTSGQAIDIQDL
Sbjct: 121 GIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRSAVKLAKIVSEASTSGQAIDIQDL 180

Query: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240
           FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG
Sbjct: 181 FMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIG 240

Query: 241 SEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDII 300
           SEATLKENIKIVDEYIYKLIH+ LEQRQKL DKTSMRRENIVSRFLQAET DPKYLRDII
Sbjct: 241 SEATLKENIKIVDEYIYKLIHNTLEQRQKLCDKTSMRRENIVSRFLQAETTDPKYLRDII 300

Query: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESL 360
           LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVED+TDFA+FAARLTEESL
Sbjct: 301 LNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDVTDFAQFAARLTEESL 360

Query: 361 EKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420
           EKMQFLTATINETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG
Sbjct: 361 EKMQFLTATINETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWG 420

Query: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480
           DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK
Sbjct: 421 DDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFK 480

Query: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 513
           MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS
Sbjct: 481 MSDEKKIVKYQPMINLLIKGGLQVCAIPRSPNHFNS 516

BLAST of Cp4.1LG03g16900 vs. ExPASy TrEMBL
Match: A0A6J1E944 (cytochrome P450 704C1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111431924 PE=3 SV=1)

HSP 1 Score: 945 bits (2442), Expect = 0.0
Identity = 472/488 (96.72%), Postives = 477/488 (97.75%), Query Frame = 0

Query: 33  QILTRKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLARKHKTYRLLGPFRNEIYTSDPPN 92
           QIL RKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLARKHKTYRLLGPFRNEIYTSDPPN
Sbjct: 9   QILARKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLARKHKTYRLLGPFRNEIYTSDPPN 68

Query: 93  VEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQRKISSYEFSTRVLREYSSV 152
           VEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQRKISSYEFSTRVLREYSSV
Sbjct: 69  VEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQRKISSYEFSTRVLREYSSV 128

Query: 153 IFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVSFGIDLDSLHGVNEETGKFC 212
           IFRR AVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVSFGIDLDSLHGVNEETGKFC
Sbjct: 129 IFRRSAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVSFGIDLDSLHGVNEETGKFC 188

Query: 213 NAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKIVDEYIYKLIHSALEQRQKLGD 272
           NAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKIVDEYIYKLIH+ALEQRQKLGD
Sbjct: 189 NAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKIVDEYIYKLIHNALEQRQKLGD 248

Query: 273 KTS----MRRENIVSRFLQAETADPKYLRDIILNFVIAGKDTTAVTLAWFIYMLCKHPAV 332
           KTS    MRRENIVSRFLQAET DPKYLRDIILNFVIAGKDTTAVTLAWFIYMLCKHPAV
Sbjct: 249 KTSVDEFMRRENIVSRFLQAETTDPKYLRDIILNFVIAGKDTTAVTLAWFIYMLCKHPAV 308

Query: 333 QERVAKEIKEVTNVEDITDFAEFAARLTEESLEKMQFLTATINETLRLYPAVPV---ICF 392
           QERVAKEI+EVTNVED+TDFAEFAARLTEESLEKMQFLTATINETLRLYPAVPV   ICF
Sbjct: 309 QERVAKEIEEVTNVEDVTDFAEFAARLTEESLEKMQFLTATINETLRLYPAVPVDAKICF 368

Query: 393 SDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFRPERWLDGDGNVQPQSPFKFTA 452
           SDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFRPERWLDGDGNVQPQSPFKFTA
Sbjct: 369 SDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFRPERWLDGDGNVQPQSPFKFTA 428

Query: 453 FQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEKKIVKYQPMINLLIKGGLQVCAIP 512
           FQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDE+KIVKYQPMINLLIKGGLQVCA P
Sbjct: 429 FQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEEKIVKYQPMINLLIKGGLQVCATP 488

BLAST of Cp4.1LG03g16900 vs. ExPASy TrEMBL
Match: A0A0A0L912 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa_3G124940 PE=3 SV=1)

HSP 1 Score: 885 bits (2286), Expect = 0.0
Identity = 439/500 (87.80%), Postives = 469/500 (93.80%), Query Frame = 0

Query: 12  KFLSNPISVAALLLLISIVVWQILTRKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLARK 71
           +FLSNPI VAA+LLLISIVV QILTRKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLARK
Sbjct: 2   EFLSNPILVAAVLLLISIVVMQILTRKLRKKKYHPIAGTVFHQLLNFHRLHDYMTDLARK 61

Query: 72  HKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWR 131
           HKTY+LLGPFR+E+YTSDPPNVEYMLRTNFENFGKGSHN+RILRDLLGDGIFTVDGEKWR
Sbjct: 62  HKTYKLLGPFRDEVYTSDPPNVEYMLRTNFENFGKGSHNYRILRDLLGDGIFTVDGEKWR 121

Query: 132 QQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFH 191
           +QRKISSYEFST+VLR+YS+VIFR+ AVKLA IVS+A+ S + IDIQDLFMKSTLDSIFH
Sbjct: 122 EQRKISSYEFSTKVLRDYSTVIFRKTAVKLANIVSEAAISNRVIDIQDLFMKSTLDSIFH 181

Query: 192 VSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKI 251
           VSFGIDL+SLHG NEE  KFC+AFEASST TLWRYVDV+WP+KK LNIGSEA LKEN+KI
Sbjct: 182 VSFGIDLESLHGSNEEISKFCSAFEASSTNTLWRYVDVSWPIKKALNIGSEAVLKENMKI 241

Query: 252 VDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADPKYLRDIILNFVIAGKDTT 311
           VDEYIYKLIHSALEQRQKLGD   MRRENIVSRFLQAET DPKYLRDIILNFVIAGKDTT
Sbjct: 242 VDEYIYKLIHSALEQRQKLGDNALMRRENIVSRFLQAETTDPKYLRDIILNFVIAGKDTT 301

Query: 312 AVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTEESLEKMQFLTATIN 371
           AVTLAWFI MLCKHP+VQERVA+EI+EVTNV D+TDF+EFAARLTEE+LEKMQFL ATIN
Sbjct: 302 AVTLAWFICMLCKHPSVQERVAREIEEVTNVGDVTDFSEFAARLTEEALEKMQFLIATIN 361

Query: 372 ETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFRPERW 431
           ETLRLYPAVPV   ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEF PERW
Sbjct: 362 ETLRLYPAVPVDAKICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFLPERW 421

Query: 432 LDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEKKIVKYQ 491
           LDGDG  QPQSPFKFTAFQAGPRIC+GKEFAYRQIKI  ++L+  FKFKMSDEKKIV+YQ
Sbjct: 422 LDGDGKFQPQSPFKFTAFQAGPRICLGKEFAYRQIKIFSALLLMFFKFKMSDEKKIVEYQ 481

Query: 492 PMINLLIKGGLQVCAIPRSP 508
           PMINLLIKGGLQVCAIPR P
Sbjct: 482 PMINLLIKGGLQVCAIPRRP 501

BLAST of Cp4.1LG03g16900 vs. TAIR 10
Match: AT2G45510.1 (cytochrome P450, family 704, subfamily A, polypeptide 2 )

HSP 1 Score: 485.0 bits (1247), Expect = 7.7e-137
Identity = 257/517 (49.71%), Postives = 353/517 (68.28%), Query Frame = 0

Query: 1   MICSVATMVAAKFLSNPISVAALLLLISIVVWQILTRKLRK-KKYHPIAGTVFHQLLNFH 60
           ++ S+A  VA        ++  +L     ++ +I T K R  K+Y P+  TVF  L +  
Sbjct: 3   ILTSIAITVAT-------TIFIVLCFTIYLMIRIFTGKSRNDKRYAPVHATVFDLLFHSD 62

Query: 61  RLHDYMTDLARKHKTYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLG 120
            L+DY T++AR+  TYR L P ++EI T+DP NVE++L+T F+N+ KG  +   + DLLG
Sbjct: 63  ELYDYETEIAREKPTYRFLSPGQSEILTADPRNVEHILKTRFDNYSKGHSSRENMADLLG 122

Query: 121 DGIFTVDGEKWRQQRKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQD 180
            GIF VDGEKWRQQRK+SS+EFSTRVLR++S  +FRR A KL   VS+ + SG+A D QD
Sbjct: 123 HGIFAVDGEKWRQQRKLSSFEFSTRVLRDFSCSVFRRNASKLVGFVSEFALSGKAFDAQD 182

Query: 181 LFMKSTLDSIFHVSFGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNI 240
           L M+ TLDSIF V FG++L  L G ++E  +F  AF+  +  T  R++D  W LK   NI
Sbjct: 183 LLMRCTLDSIFKVGFGVELKCLDGFSKEGQEFMEAFDEGNVATSSRFIDPLWKLKWFFNI 242

Query: 241 GSEATLKENIKIVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADP-----K 300
           GS++ LK++I  +D+++Y LI +  ++  K  ++ ++ RE+I+SRFL     DP     K
Sbjct: 243 GSQSKLKKSIATIDKFVYSLITTKRKELAK--EQNTVVREDILSRFLVESEKDPENMNDK 302

Query: 301 YLRDIILNFVIAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVT-NVEDITDFAEFAA 360
           YLRDIILNF+IAGKDTTA  L+WF+YMLCK+P VQE++ +EI++VT + E  TD   F  
Sbjct: 303 YLRDIILNFMIAGKDTTAALLSWFLYMLCKNPLVQEKIVQEIRDVTFSHEKTTDVNGFVE 362

Query: 361 RLTEESLEKMQFLTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMG 420
            + EE+L++M +L A ++ETLRLYP VPV      +DD LPDG  V KGD + Y  YAMG
Sbjct: 363 SINEEALDEMHYLHAALSETLRLYPPVPVDMRCAENDDVLPDGHRVSKGDNIYYIAYAMG 422

Query: 421 RMKFIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVL 480
           RM +IWG DAEEF+PERWL  DG  QP+SPFKF +F AGPRIC+GK+FAYRQ+KI+   L
Sbjct: 423 RMTYIWGQDAEEFKPERWLK-DGLFQPESPFKFISFHAGPRICLGKDFAYRQMKIVSMAL 482

Query: 481 VGSFKFKMSDEKKIVKYQPMINLLIKGGLQVCAIPRS 508
           +  F+FKM+DE   V Y+ M+ L + GGL +CAIPR+
Sbjct: 483 LHFFRFKMADENSKVYYKRMLTLHVDGGLHLCAIPRT 509

BLAST of Cp4.1LG03g16900 vs. TAIR 10
Match: AT2G44890.1 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 473.8 bits (1218), Expect = 1.8e-133
Identity = 248/503 (49.30%), Postives = 343/503 (68.19%), Query Frame = 0

Query: 18  ISVAALLLLISIVVW---QILTRKLRK-KKYHPIAGTVFHQLLNFHRLHDYMTDLARKHK 77
           I V  + +L+S  ++   +I T K R  K+Y P+  T+F    + H+L+DY T++AR   
Sbjct: 4   IVVTTIFILLSFALYLTIRIFTGKSRNDKRYTPVHATIFDLFFHSHKLYDYETEIARTKP 63

Query: 78  TYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQ 137
           T+R L P ++EI+T+DP NVE++L+T F N+ KG      L DLLG GIF VDGEKW+QQ
Sbjct: 64  TFRFLSPGQSEIFTADPRNVEHILKTRFHNYSKGPVGTVNLADLLGHGIFAVDGEKWKQQ 123

Query: 138 RKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVS 197
           RK+ S+EFSTRVLR +S  +FR  A KL   +++ + SG++ D QD+ MK TLDSIF V 
Sbjct: 124 RKLVSFEFSTRVLRNFSYSVFRTSASKLVGFIAEFALSGKSFDFQDMLMKCTLDSIFKVG 183

Query: 198 FGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKIVD 257
           FG++L  L G ++E  +F  AF+  +  T  R  D  W LK  LNIGSE+ LK++I I+D
Sbjct: 184 FGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESRLKKSIAIID 243

Query: 258 EYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADP-----KYLRDIILNFVIAGK 317
           +++Y LI +  ++  K  ++ +  RE+I+S+FL     DP     KYLRDIILN ++AGK
Sbjct: 244 KFVYSLITTKRKELSK--EQNTSVREDILSKFLLESEKDPENMNDKYLRDIILNVMVAGK 303

Query: 318 DTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNV-EDITDFAEFAARLTEESLEKMQFLT 377
           DTTA +L+WF+YMLCK+P VQE++ +EI++VT+  E  TD   F   +TEE+L +MQ+L 
Sbjct: 304 DTTAASLSWFLYMLCKNPLVQEKIVQEIRDVTSSHEKTTDVNGFIESVTEEALAQMQYLH 363

Query: 378 ATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFR 437
           A ++ET+RLYP VP       +DD LPDG  V KGD + Y  YAMGRM +IWG DAEEF+
Sbjct: 364 AALSETMRLYPPVPEHMRCAENDDVLPDGHRVSKGDNIYYISYAMGRMTYIWGQDAEEFK 423

Query: 438 PERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEKKI 497
           PERWL  DG  QP+S FKF +F AGPRIC+GK+FAYRQ+KI+   L+  F+FKM+DE   
Sbjct: 424 PERWLK-DGVFQPESQFKFISFHAGPRICIGKDFAYRQMKIVSMALLHFFRFKMADENSK 483

Query: 498 VKYQPMINLLIKGGLQVCAIPRS 508
           V Y+ M+ L + GGL +CAIPR+
Sbjct: 484 VSYKKMLTLHVDGGLHLCAIPRT 503

BLAST of Cp4.1LG03g16900 vs. TAIR 10
Match: AT2G44890.2 (cytochrome P450, family 704, subfamily A, polypeptide 1 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.6e-124
Identity = 239/503 (47.51%), Postives = 330/503 (65.61%), Query Frame = 0

Query: 18  ISVAALLLLISIVVW---QILTRKLRK-KKYHPIAGTVFHQLLNFHRLHDYMTDLARKHK 77
           I V  + +L+S  ++   +I T K R  K+Y P+  T+F    + H+L+DY T++AR   
Sbjct: 10  IVVTTIFILLSFALYLTIRIFTGKSRNDKRYTPVHATIFDLFFHSHKLYDYETEIARTKP 69

Query: 78  TYRLLGPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQ 137
           T+R L P ++EI+T+DP NVE++L+T F N+ KG      L DLLG GIF VDGEKW+QQ
Sbjct: 70  TFRFLSPGQSEIFTADPRNVEHILKTRFHNYSKGPVGTVNLADLLGHGIFAVDGEKWKQQ 129

Query: 138 RKISSYEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVS 197
           RK+ S+EFSTRVLR +S  +FR  A KL   +++ + SG++ D QD+ MK TLDSIF V 
Sbjct: 130 RKLVSFEFSTRVLRNFSYSVFRTSASKLVGFIAEFALSGKSFDFQDMLMKCTLDSIFKVG 189

Query: 198 FGIDLDSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKIVD 257
           FG++L  L G ++E  +F  AF+  +  T  R  D  W LK  LNIGSE+ LK++I I+D
Sbjct: 190 FGVELGCLDGFSKEGEEFMKAFDEGNGATSSRVTDPFWKLKCFLNIGSESRLKKSIAIID 249

Query: 258 EYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADP-----KYLRDIILNFVIAGK 317
           +++Y LI +  ++  K       +  +I+S+FL     DP     KYLRDIILN ++AGK
Sbjct: 250 KFVYSLITTKRKELSK------EQNTDILSKFLLESEKDPENMNDKYLRDIILNVMVAGK 309

Query: 318 DTTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNV-EDITDFAEFAARLTEESLEKMQFLT 377
           DTTA +L+WF+YMLCK+P VQE++ +EI++VT+  E  TD   F   +TEE+L +MQ+L 
Sbjct: 310 DTTAASLSWFLYMLCKNPLVQEKIVQEIRDVTSSHEKTTDVNGFIESVTEEALAQMQYLH 369

Query: 378 ATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEEFR 437
           A ++ET+RLYP VP       +DD LPDG  V KGD + Y  YAMGRM +IWG DAEEF+
Sbjct: 370 AALSETMRLYPPVPEHMRCAENDDVLPDGHRVSKGDNIYYISYAMGRMTYIWGQDAEEFK 429

Query: 438 PERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDEKKI 497
           PERWL  DG              AGPRIC+GK+FAYRQ+KI+   L+  F+FKM+DE   
Sbjct: 430 PERWLK-DG--------------AGPRICIGKDFAYRQMKIVSMALLHFFRFKMADENSK 489

Query: 498 VKYQPMINLLIKGGLQVCAIPRS 508
           V Y+ M+ L + GGL +CAIPR+
Sbjct: 490 VSYKKMLTLHVDGGLHLCAIPRT 491

BLAST of Cp4.1LG03g16900 vs. TAIR 10
Match: AT1G69500.1 (cytochrome P450, family 704, subfamily B, polypeptide 1 )

HSP 1 Score: 377.1 bits (967), Expect = 2.3e-104
Identity = 216/524 (41.22%), Postives = 313/524 (59.73%), Query Frame = 0

Query: 23  LLLLISIVVWQILTRKLRKK----KYHPIAGTVFHQLLNFHRLHDYMTDLARKHKTYRLL 82
           L++   +  W  L R  ++     K  P+ G    QL NF R+HD++ +     +T  + 
Sbjct: 5   LVIACMVTSWIFLHRWGQRNKSGPKTWPLVGAAIEQLTNFDRMHDWLVEYLYNSRTVVVP 64

Query: 83  GPFRNEIYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQRKISS 142
            PF    Y +DP NVEY+L+TNF N+ KG   H  +  LLGDGIF  DGE WR+QRK +S
Sbjct: 65  MPFTTYTYIADPINVEYVLKTNFSNYPKGETYHSYMEVLLGDGIFNSDGELWRKQRKTAS 124

Query: 143 YEFSTRVLREYSSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVSFGIDL 202
           +EF+++ LR++S+V+F+ Y++KL  I+S+AS   Q +D+Q+L M+ TLDSI  V FG+++
Sbjct: 125 FEFASKNLRDFSTVVFKEYSLKLFTILSQASFKEQQVDMQELLMRMTLDSICKVGFGVEI 184

Query: 203 DSLHGVNEETGKFCNAFEASSTMTLWRYVDVAWPLKKILNIGSEATLKENIKIVDEYIY- 262
            +L     E   F  AF+ ++ +   R++D  W +KK LNIGSEA L ++IK+V+++ Y 
Sbjct: 185 GTLAPELPE-NHFAKAFDTANIIVTLRFIDPLWKMKKFLNIGSEALLGKSIKVVNDFTYS 244

Query: 263 -------KLIHSALEQRQKLGDKTSMRRENIVSRFLQAETADP------KYLRDIILNFV 322
                  +L+ + +       +  +  + +I+SRF++  + DP      K LRDI+LNFV
Sbjct: 245 VIRRRKAELLEAQISPTNNNNNNNNKVKHDILSRFIEI-SDDPDSKETEKSLRDIVLNFV 304

Query: 323 IAGKDTTAVTLAWFIYMLCKHPAVQERVAKEIKEVT--------------NVEDITDF-- 382
           IAG+DTTA TL W IYM+  +  V E++  E++E+               + ED   F  
Sbjct: 305 IAGRDTTATTLTWAIYMIMMNENVAEKLYSELQELEKESAEATNTSLHQYDTEDFNSFNE 364

Query: 383 --AEFAARLTEESLEKMQFLTATINETLRLYPAVPVI---CFSDDTLPDGFSVRKGDMVS 442
              EFA  L  +SL K+ +L A I ETLRLYPAVP        DD LP+G  V+ G MV+
Sbjct: 365 KVTEFAGLLNYDSLGKLHYLHAVITETLRLYPAVPQDPKGVLEDDMLPNGTKVKAGGMVT 424

Query: 443 YQPYAMGRMKFIWGDDAEEFRPERWLDGDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQI 502
           Y PY+MGRM++ WG DA  F+PERWL  DG  Q  SPFKFTAFQAGPRIC+GK+ AY Q+
Sbjct: 425 YVPYSMGRMEYNWGSDAALFKPERWLK-DGVFQNASPFKFTAFQAGPRICLGKDSAYLQM 484

Query: 503 KIMLSVLVGSFKFKMSDEKKIVKYQPMINLLIKGGLQVCAIPRS 508
           K+ +++L   +KF +      VKY+ M  L +  GL+V    RS
Sbjct: 485 KMAMAILCRFYKFHLVPNHP-VKYRMMTILSMAHGLKVTVSRRS 524

BLAST of Cp4.1LG03g16900 vs. TAIR 10
Match: AT3G56630.1 (cytochrome P450, family 94, subfamily D, polypeptide 2 )

HSP 1 Score: 313.5 bits (802), Expect = 3.1e-85
Identity = 185/506 (36.56%), Postives = 293/506 (57.91%), Query Frame = 0

Query: 24  LLLISIVVWQILTRKLRKKKY----HPIAGTVFHQLLNFHRLHDYMTDLARKHKTYRLLG 83
           LL   IV++ I   K    ++    +PI G++   + N HR  D+  +   +  T   + 
Sbjct: 9   LLCFPIVIFFIFFPKNSSSEFGFKSYPIVGSLPGLVNNRHRFLDWTVETLSRCPTQTAI- 68

Query: 84  PFRNE-----IYTSDPPNVEYMLRTNFENFGKGSHNHRILRDLLGDGIFTVDGEKWRQQR 143
            FR       + T++P NVEYML+T FE+F KG     IL D LG GIF  DGE W +QR
Sbjct: 69  -FRRPGKLQFVMTANPANVEYMLKTKFESFPKGERFISILEDFLGRGIFNSDGEMWWKQR 128

Query: 144 KISSYEFSTRVLREY-SSVIFRRYAVKLAKIVSKASTSGQAIDIQDLFMKSTLDSIFHVS 203
           K +SYEFST+ LR++  S +      +L  ++++A+T+G+ ID+QD+  +   D+I  ++
Sbjct: 129 KTASYEFSTKSLRDFVMSNVTVEINTRLVPVLAEAATNGKLIDLQDILERFAFDNICKLA 188

Query: 204 FGIDLDSLHGVNEETGKFCNAFEASSTMTLWRY---VDVAWPLKKILNIGSEATLKENIK 263
           F +D   L         F  AFE ++T+   R+   +  +W +KK LNIGSE  L+E+I 
Sbjct: 189 FNVDSACLGDDGAAGVNFMQAFETAATIISQRFQSVISYSWKIKKKLNIGSERVLRESIM 248

Query: 264 IVDEYIYKLIHSALEQRQKLGDKTSMRRENIVSRFLQAETAD-PKYLRDIILNFVIAGKD 323
           IV ++  +++ + +EQ      K S  +E+++SRF+  E  + P+ LRDI+++F++AG+D
Sbjct: 249 IVHKFADEIVRNRIEQ-----GKVSDHKEDLLSRFISKEEMNSPEILRDIVISFILAGRD 308

Query: 324 TTAVTLAWFIYMLCKHPAVQERVAKEIKEVTNVEDITDFAEFAARLTE----ESLEKMQF 383
           TT+  L+WF ++L  HP V++++ +E+  +              R+ E    E L+ M +
Sbjct: 309 TTSSALSWFFWLLSMHPEVKDKILQELNSIRE--------RTGKRIGEVYGFEDLKLMNY 368

Query: 384 LTATINETLRLYPAVPV---ICFSDDTLPDGFSVRKGDMVSYQPYAMGRMKFIWGDDAEE 443
           L A I E+LRLYP VPV    C  D+ LPDG  + K   +SY  YAMGRM+ IWG D + 
Sbjct: 369 LHAAITESLRLYPPVPVDTMSCAEDNVLPDGTFIGKDWGISYNAYAMGRMESIWGKDCDR 428

Query: 444 FRPERWLD-GDGNVQPQSPFKFTAFQAGPRICMGKEFAYRQIKIMLSVLVGSFKFKMSDE 503
           F PERW+D  +G  + ++P+KF AF AGPR+C+GKE AY Q+K +++ ++  F  ++  +
Sbjct: 429 FDPERWIDETNGGFRGENPYKFPAFHAGPRMCLGKEMAYIQMKSIVAAVLERFVVEVPGK 488

Query: 504 KKIVKYQPMINLLIKGGLQVCAIPRS 508
           K+  +    + L I+GGL V    RS
Sbjct: 489 KERPEILMSVTLRIRGGLNVRVQERS 499