Cp4.1LG03g14780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g14780
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Alpha/beta-Hydrolases superfamily protein
Location	Cp4.1LG03: 8467518 .. 8470939 (+)
RNA-Seq Expression	Cp4.1LG03g14780
Synteny	Cp4.1LG03g14780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATGCGGGAAGGTTGAAATTAACGAAGCTTTTCACACTCAAACAGTGGGTTTAATGCCGATTTGCGTCTTCTTTACATTTGAGGATACATCCGATATACGCGTATTAGATGATAAGTTCAGTTGGGGACGCAGAATGGGCTAAGGTACCGATATAACCGCATCCCTTTTACTTTTCTTGATATATACGAATTGCTGGCTGTTCATCTGGAGGCGTCTTCTTTAGATTCATACTAAAAGGGGTTTCCTAAACATGGCTACTACCCGTAACAATGCTGGTAACGACGGCGATGATTTTAGATACCTTGTTGTGCGGCCGGAGAAGGGTGGAATCAGAGACGTTTTCCGGTGTTGGGCTTGGCCGGATGGCGATGGTGGACGGAGATTCTTCGAGGGCTCCGAGGTGGGTGTTTTTGGTGCGATGGTTTTGGGACGCAGGTGGGTCATAGTGGTGTCGATTTTGATTCGAAAAGTGATAGCCGCTCTGGCGAAACCAATGGAATGGACTGGCAATGTGGTGGAATTCAGTCTCAATCTTCTGTCTATGAATGGCAATATCTCGGGATTGTTCTGCAACATCGTGCGCGGTACGGGTCCACATGCTTTTGAATCTCTACCAACTTGGAACATCACATGTCTAGATTTTACAGAATAGGCATTGAAATCTCTGCTATTTTTGGATGCGCAGGTGAAGTGGTAGTGCCACAGAGGGGCACAGACACATTTATCAGCACCATTGGGCTGTTAGATGGGCGAATGAATCTATTAAATGAAGAACAAATTCTTGAAGGAGCTACTGATTCTGTTTATGAAGAAAGAGGCTTCAGACTTGAAATGGGCAATCGGACTCTAATGGACCTTTGCATCATGGCTTCAAAACTATCCTACGAGAATGAAAAGGTCATTCAGAATGTTGTTCTTCGCCGCTGGAAGGCAGGTTATTTCATTGCTTCTCATGTTGTAATTACGTGAGATCCCACATCGATTGAGGAGGTGAACAAAACACCTTTTATAAGGGTGTGGAAACCTCTCACTAACAGATGTGTTTTAGAAACCTTGAGGAGAAGCCCGAAAGGGAAATCTCAAAGAAGACAATATCTTCTAGCGGTGGGCTTGGACTGTTAAAAATGGTATCAAAGCTAGACACCGAGCGATGTGTCAACGAGGAGGCTGAGCCACGAAGGGGGTAGACATGTGGCAATGTGCCAACAAAGATGTTGGCCCTCTGAGGGGGTGGACTTGGTGGGGGTCCCACATCGATTGGAGAAAGGAACGAGTGCTAGCGAGGACGCTGGGCCTCGAAGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGAGAACGAAACACCCTTTATAAGTGTGTGGAAAGCTCTTCCTAGCATAGACGTTTTAAAAACCTTGAGGGGTGTCCTAGCATACGCGTTTTAAAAACTTAGAGAAGCATCCCAGAGAGGACAAGTTCTGTTAGCGGTGGGCTTGAGCGGTTACAATTTATGTGTCATTTCAGACTAACTTTGATCGTTAATAATGAAGCAGATGCATTTCGTGGGATTCTACAATTGCTGGAATGGTAAGAACAAAACTTGCATGATCATCTAGTGGAAAATACTGTCTTTAGTTTCATTACTTAAGCCAACTAATTGAATAACCTGGTTCTGTTTGTCTCTTGACCACCACAAAATTTGGTAGATTTCCTAAAGGACAGCTCTACGCAGGTGTTCATACTGTGTGATAAGCCTAAAGACGCCAATCTGATATTGATCAGCTTCAGGGGCACAGAACCTTTTGATGCAGATGACTGGTGCACTGATTTTGACTACTCTTGGTATGAGATCCCGGAAGTTGGGAAGATTCACATAGGCTTCTTAGAAGCTCTTGGTTTGGGCAACAGAAATGATGCCAACAGCTTCAAAGGTCACCATAAGTTAAGCTCCATTTCTTCAGACCTTTCCAACATTGAACAGGATAGATTACATCCATCTTTGGACTCTGAACAGTTCGACGCGGTTACGCCCGAAATGGTGCAGTTGTCTGCATACTATGCAGTGAAACTTAAGCTCAGGAGATTGCTTATGGAGCATAAAAATGCGAAGTTTGTGGTCACTGGTCATAGTTTAGGCGGGGCGCTTGCAGTTCTGTTCCCTACAGTTCTGGTGCTGCATAAGGAAATGGAAATAATGGACAGGCTATTAGGTGTATATACATTTGGGCAGCCTAGGATCGGCGACAAGCAACTGGCGCAGTTCATGGAGGCTCATTTGATGAAGCCTATTCCAAGGTACTTCAGGGTTGTCTACTGCAATGACCTTGTACCTAGGCTGCCTTACGACGATAAAGCTTTTTTCTATAAGCATTTTGGGGTTTGTCTATACTACGACAGCCTATTCACTGAGAATGTAAGTACATTTTCTGCCTCCTGTTTCTTGTAGTTTACTAAGCTAGAATTTGTAATAATGAACCTGAATCATATTAAGAATAAAATGTGCTGGTATGCTGTTGCTACTTGTGAGGTCCCACATCAGTTGGAGAGGAGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGTAGACACGTTTTAAAAACTTTGAGGGGAAGCCTAAAAGGAAAAGTCTAAAGAGGACAATATCTGCTAGCGGTGGCTTGAGTTGTTACAAATGGTATTAGAGTTAGACATCGAGTGGTGTGCCAGCAAAGATGCTGGGCTCCCAAGAGGGATGGATTATGAGGTCCCACAACGAGTGGAGAGGGGATGAAAGCATTTCTTATAAGGGTGTGGAAACCTCTTCGTAGTAGACACGTTTTAAAAACCTTGAGGAAAAACCCAAAAGGAAAAACCTAAAAGGGACAATATCTGCTAATAATGGGCTTAGACTCTTACACTATTAGTCGCCCCAATTTGTGGTTCTTATTGTGTGAATTTGTTTTTGCAATCTGCATATTTGAAGGAAGTAGATGAGGAATCGAACAAGAACTTCTTTGGGATTAGATACCTGATACCGGAATATCTCAATGCTTTATGGGAGCTGATCAGAAGCTTGCTGATGGGCTACACATATGGAGCTGAGTACAGGGAGGGATGGTTTTGCTTATTGCTAAGGGTAATAGGACTGGCACTTCCTGGCATTTCTGCTCATTGCCTCACAAATTATATCGACTCTGTGAGGCTTGGTAAGAAGAAGAACTCTCTTTGATGATTCCAATTATTGCTCACTTGGCAAGACAACAACAGCAAATCCACTTGGAGAAATTTTAAGGTTAGCAGTTTTCTGACCCAGATACGTTTTATGAGTAGGATAATTGTTTGTCGACATATACTGGGGAACAATATTTACTACAGGCTTTTCTCATCAGAAGAGGAATAAAAAACTTAACTTTCTTTATGATTAATTGCTAAGTGGGATTGGATTTGATA

mRNA sequence

TATGCGGGAAGGTTGAAATTAACGAAGCTTTTCACACTCAAACAGTGGGTTTAATGCCGATTTGCGTCTTCTTTACATTTGAGGATACATCCGATATACGCGTATTAGATGATAAGTTCAGTTGGGGACGCAGAATGGGCTAAGGTACCGATATAACCGCATCCCTTTTACTTTTCTTGATATATACGAATTGCTGGCTGTTCATCTGGAGGCGTCTTCTTTAGATTCATACTAAAAGGGGTTTCCTAAACATGGCTACTACCCGTAACAATGCTGGTAACGACGGCGATGATTTTAGATACCTTGTTGTGCGGCCGGAGAAGGGTGGAATCAGAGACGTTTTCCGGTGTTGGGCTTGGCCGGATGGCGATGGTGGACGGAGATTCTTCGAGGGCTCCGAGGTGGGTGTTTTTGGTGCGATGGTTTTGGGACGCAGGTGGGTCATAGTGGTGTCGATTTTGATTCGAAAAGTGATAGCCGCTCTGGCGAAACCAATGGAATGGACTGGCAATGTGGTGGAATTCAGTCTCAATCTTCTGTCTATGAATGGCAATATCTCGGGATTGTTCTGCAACATCGTGCGCGGTGAAGTGGTAGTGCCACAGAGGGGCACAGACACATTTATCAGCACCATTGGGCTGTTAGATGGGCGAATGAATCTATTAAATGAAGAACAAATTCTTGAAGGAGCTACTGATTCTGTTTATGAAGAAAGAGGCTTCAGACTTGAAATGGGCAATCGGACTCTAATGGACCTTTGCATCATGGCTTCAAAACTATCCTACGAGAATGAAAAGGACAGCTCTACGCAGGTGTTCATACTGTGTGATAAGCCTAAAGACGCCAATCTGATATTGATCAGCTTCAGGGGCACAGAACCTTTTGATGCAGATGACTGGTGCACTGATTTTGACTACTCTTGGTATGAGATCCCGGAAGTTGGGAAGATTCACATAGGCTTCTTAGAAGCTCTTGGTTTGGGCAACAGAAATGATGCCAACAGCTTCAAAGGTCACCATAAGTTAAGCTCCATTTCTTCAGACCTTTCCAACATTGAACAGGATAGATTACATCCATCTTTGGACTCTGAACAGTTCGACGCGGTTACGCCCGAAATGGTGCAGTTGTCTGCATACTATGCAGTGAAACTTAAGCTCAGGAGATTGCTTATGGAGCATAAAAATGCGAAGTTTGTGGTCACTGGTCATAGTTTAGGCGGGGCGCTTGCAGTTCTGTTCCCTACAGTTCTGGTGCTGCATAAGGAAATGGAAATAATGGACAGGCTATTAGGTGTATATACATTTGGGCAGCCTAGGATCGGCGACAAGCAACTGGCGCAGTTCATGGAGGCTCATTTGATGAAGCCTATTCCAAGGTACTTCAGGGTTGTCTACTGCAATGACCTTGTACCTAGGCTGCCTTACGACGATAAAGCTTTTTTCTATAAGCATTTTGGGGTTTGTCTATACTACGACAGCCTATTCACTGAGAATGAAGTAGATGAGGAATCGAACAAGAACTTCTTTGGGATTAGATACCTGATACCGGAATATCTCAATGCTTTATGGGAGCTGATCAGAAGCTTGCTGATGGGCTACACATATGGAGCTGAGTACAGGGAGGGATGGTTTTGCTTATTGCTAAGGGTAATAGGACTGGCACTTCCTGGCATTTCTGCTCATTGCCTCACAAATTATATCGACTCTGTGAGGCTTGGTAAGAAGAAGAACTCTCTTTGATGATTCCAATTATTGCTCACTTGGCAAGACAACAACAGCAAATCCACTTGGAGAAATTTTAAGGTTAGCAGTTTTCTGACCCAGATACGTTTTATGAGTAGGATAATTGTTTGTCGACATATACTGGGGAACAATATTTACTACAGGCTTTTCTCATCAGAAGAGGAATAAAAAACTTAACTTTCTTTATGATTAATTGCTAAGTGGGATTGGATTTGATA

Coding sequence (CDS)

ATGGCTACTACCCGTAACAATGCTGGTAACGACGGCGATGATTTTAGATACCTTGTTGTGCGGCCGGAGAAGGGTGGAATCAGAGACGTTTTCCGGTGTTGGGCTTGGCCGGATGGCGATGGTGGACGGAGATTCTTCGAGGGCTCCGAGGTGGGTGTTTTTGGTGCGATGGTTTTGGGACGCAGGTGGGTCATAGTGGTGTCGATTTTGATTCGAAAAGTGATAGCCGCTCTGGCGAAACCAATGGAATGGACTGGCAATGTGGTGGAATTCAGTCTCAATCTTCTGTCTATGAATGGCAATATCTCGGGATTGTTCTGCAACATCGTGCGCGGTGAAGTGGTAGTGCCACAGAGGGGCACAGACACATTTATCAGCACCATTGGGCTGTTAGATGGGCGAATGAATCTATTAAATGAAGAACAAATTCTTGAAGGAGCTACTGATTCTGTTTATGAAGAAAGAGGCTTCAGACTTGAAATGGGCAATCGGACTCTAATGGACCTTTGCATCATGGCTTCAAAACTATCCTACGAGAATGAAAAGGACAGCTCTACGCAGGTGTTCATACTGTGTGATAAGCCTAAAGACGCCAATCTGATATTGATCAGCTTCAGGGGCACAGAACCTTTTGATGCAGATGACTGGTGCACTGATTTTGACTACTCTTGGTATGAGATCCCGGAAGTTGGGAAGATTCACATAGGCTTCTTAGAAGCTCTTGGTTTGGGCAACAGAAATGATGCCAACAGCTTCAAAGGTCACCATAAGTTAAGCTCCATTTCTTCAGACCTTTCCAACATTGAACAGGATAGATTACATCCATCTTTGGACTCTGAACAGTTCGACGCGGTTACGCCCGAAATGGTGCAGTTGTCTGCATACTATGCAGTGAAACTTAAGCTCAGGAGATTGCTTATGGAGCATAAAAATGCGAAGTTTGTGGTCACTGGTCATAGTTTAGGCGGGGCGCTTGCAGTTCTGTTCCCTACAGTTCTGGTGCTGCATAAGGAAATGGAAATAATGGACAGGCTATTAGGTGTATATACATTTGGGCAGCCTAGGATCGGCGACAAGCAACTGGCGCAGTTCATGGAGGCTCATTTGATGAAGCCTATTCCAAGGTACTTCAGGGTTGTCTACTGCAATGACCTTGTACCTAGGCTGCCTTACGACGATAAAGCTTTTTTCTATAAGCATTTTGGGGTTTGTCTATACTACGACAGCCTATTCACTGAGAATGAAGTAGATGAGGAATCGAACAAGAACTTCTTTGGGATTAGATACCTGATACCGGAATATCTCAATGCTTTATGGGAGCTGATCAGAAGCTTGCTGATGGGCTACACATATGGAGCTGAGTACAGGGAGGGATGGTTTTGCTTATTGCTAAGGGTAATAGGACTGGCACTTCCTGGCATTTCTGCTCATTGCCTCACAAATTATATCGACTCTGTGAGGCTTGGTAAGAAGAAGAACTCTCTTTGA

Protein sequence

MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLGRRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGTDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYENEKDSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL

Homology

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BLAST of Cp4.1LG03g14780 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.4e-156
Identity = 284/550 (51.64%), Postives = 370/550 (67.27%), Query Frame = 0


Query: 16  RYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVF-----------------GAMV 75
           R+L+V  E GGI D+ R   W + + G +F   S  G                   G  V
Sbjct: 23  RFLIVSHENGGIWDLVRFGVWGNKESGDKFLHYSAGGGLLEEHLVRSDDSGGGDDRGGEV 82

Query: 76  LGRRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQ 135
              RWVI VSI++RK+IA   KPMEWTG +VEF LNL S+NGN  GL  NI+ G+VV+P 
Sbjct: 83  PDHRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPH 142

Query: 136 RGTDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSY 195
           RG++TFIS IG LDGR+NL   E + +   +  + +   ++ +G+R LMDLC+MASKL+Y
Sbjct: 143 RGSETFISAIGHLDGRINLYKSETLTKEIGEPDFWQ---KIGIGHRDLMDLCMMASKLAY 202

Query: 196 EN--------------------------EKDSSTQVFILCDKPKDANLILISFRGTEPFD 255
           EN                          EK+ STQVF+LCDKPKDANLIL+SFRGTEPFD
Sbjct: 203 ENEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFD 262

Query: 256 ADDWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIE--Q 315
           ADDW TDFDYSWYEIP++GK+H+GFLEALGLGNR +A++F  H +L   +   +N+E   
Sbjct: 263 ADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTF--HEQLFVNNLKFANLENVH 322

Query: 316 DRLHPSLDSE----------------QFDAVTP----------EMVQLSAYYAVKLKLRR 375
             + PS  S+                  D+  P          E+ + +AYY V+ KL+R
Sbjct: 323 ATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKR 382

Query: 376 LLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQF 435
           LL EHKNAKFVVTGHSLGGALA+LFP VLVLH+E+++M+RLLG+YT+GQPR+G++QL +F
Sbjct: 383 LLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRF 442

Query: 436 MEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTENEVDEESNKNF 495
           MEAHL  P+P+YFRVVYCNDLVPRLPYD+K F +KHFG+C YY+SL+ E  ++EE N N+
Sbjct: 443 MEAHLEHPVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNY 502

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BLAST of Cp4.1LG03g14780 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 9.7e-150
Identity = 270/517 (52.22%), Postives = 358/517 (69.25%), Query Frame = 0


Query: 7   NAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVL-----GR 66
           +  N G    YL+VRP +GG  D+FR     D     +F E  +   +  + +       
Sbjct: 9   SGSNPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDH 68

Query: 67  RWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGT 126
           RWVIVVSIL+RK+I  L  PME+TG VV+F LNL S NG   GL   +++ +VV+P+RG+
Sbjct: 69  RWVIVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGS 128

Query: 127 DTFISTIGLLDGRMNLLNEEQILE--GATDSVYEERGFRLEMGNRTLMDLCIMASKLSYE 186
            TF+STIG LDGR++L  E   +E     DSV   R  ++E+G+R LMDLC+MASKL+YE
Sbjct: 129 VTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGR-VKIELGSRGLMDLCVMASKLAYE 188

Query: 187 N--------------------------EKDSSTQVFILCDKPKDANLILISFRGTEPFDA 246
           N                          +K  STQVF+  DK KDANLI+ISFRGTEPFDA
Sbjct: 189 NAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDA 248

Query: 247 DDWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKL-SSISSDLSNIEQDR 306
           DDW TDFDYSWYE+P VGK+H+GFLEA+GLGNR+D  +F  H+ L    SS+  N +++ 
Sbjct: 249 DDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTF--HYNLFEQTSSEEENSKKNL 308

Query: 307 LHPSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTV 366
           L              +MV+ SAYYAV++ L+RLL EH+NA+FVVTGHSLGGALA+LFPT+
Sbjct: 309 L--------------DMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTL 368

Query: 367 LVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYD 426
           LVL++E EIM RLLGVYTFGQPRIG++++  FM+A L +P+ RYFRVVYCND+VPRLPYD
Sbjct: 369 LVLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYD 428

Query: 427 DKAFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYG 486
           DK F YKHFG+CL+YDS + E + ++E + N +G+RY I  ++ A+WEL+R L MGYT+G
Sbjct: 429 DKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHG 488

Query: 487 AEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLG 490
            +Y+EGWF +L R++GL +PG+S HC+T+Y++SVRLG
Sbjct: 489 PDYKEGWFRILFRLMGLVIPGLSDHCMTDYVNSVRLG 508

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BLAST of Cp4.1LG03g14780 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 9.8e-110
Identity = 217/511 (42.47%), Postives = 294/511 (57.53%), Query Frame = 0


Query: 17  YLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLGRRWVIVVSILIRKVIA 76
           +L+V P++G   D+F+     D   G +FF  S+  V        RW   VS+ I K++ 
Sbjct: 12  HLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRW--PVSVFICKILQ 71

Query: 77  ALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGTDTFISTIGLLDGRMN 136
               P    G++ +F LN    N    G+  NI    + +P+RG   FISTIG LD RM+
Sbjct: 72  LFQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGYLDSRMD 131

Query: 137 LLN--------EEQILEGATDSVYEERGFRLE--MGNRTLMDLCIMASKLSYENEK---- 196
           L          +E I      S+ E    +++  +GNR+LMDLCIMASKL+YEN K    
Sbjct: 132 LHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYENTKVVER 191

Query: 197 ----------------------DSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDF 256
                                   +T  FI CDKPKDANLI+ISFRGT PF   +WCTDF
Sbjct: 192 VVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPNWCTDF 251

Query: 257 DYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSE 316
           D+S   + + G +H+GFLEA+GLG+RN  +SF+      S  S     ++  + P     
Sbjct: 252 DFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMAPDHLVW 311

Query: 317 QFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEME 376
            +D V         Y+     L+ LL +HKNAKFVVTGHSLGGALA+LF  +L + +E E
Sbjct: 312 AYDGV---------YFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETE 371

Query: 377 IMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKH 436
           ++DRLL VYTFGQPRIG+  L  FM+  L  P  RYFRVVYCND+VPR+P+DD  F ++H
Sbjct: 372 VLDRLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEH 431

Query: 437 FGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWF 492
           FG C+YYDS F      EE ++N FGI   I  ++ A WEL RS ++ + YGAEY+E W 
Sbjct: 432 FGTCIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWE 491

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BLAST of Cp4.1LG03g14780 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 4.1e-07
Identity = 33/103 (32.04%), Postives = 56/103 (54.37%), Query Frame = 0


Query: 298 VKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQPRIG 357
           ++ K+   + EH + + V TGHSLGGALA       V   ++      + V+++G PR+G
Sbjct: 146 LRQKVEDAVREHPDYRVVFTGHSLGGALAT------VAGADLRGNGYDIDVFSYGAPRVG 205

Query: 358 DKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKH 401
           ++  A+F+       +   +R+ + ND+VPRLP   + F Y H
Sbjct: 206 NRAFAEFLTVQTGGTL---YRITHTNDIVPRLP--PREFGYSH 237

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BLAST of Cp4.1LG03g14780 vs. ExPASy Swiss-Prot
Match: P61871 (Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 1.6e-06
Identity = 36/98 (36.73%), Postives = 55/98 (56.12%), Query Frame = 0


Query: 306 LMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQFM 365
           L  H   K +VTGHSLGGA A+L    L   +E  +  + L ++T G PR+G+   A ++
Sbjct: 254 LTAHPTYKVIVTGHSLGGAQALLAGMDL-YQREPRLSPKNLSIFTVGGPRVGNPTFAYYV 313

Query: 366 EAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGV 404
           E+     IP + R V+  D+VP +P   ++F + H GV
Sbjct: 314 ES---TGIP-FQRTVHKRDIVPHVP--PQSFGFLHPGV 344

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BLAST of Cp4.1LG03g14780 vs. NCBI nr
Match: XP_023526824.1 (uncharacterized protein LOC111790207 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1000 bits (2586), Expect = 0.0
Identity = 495/510 (97.06%), Postives = 495/510 (97.06%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG
Sbjct: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN
Sbjct: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180

Query: 181 EK---------------DSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWY 240
           EK               DSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWY
Sbjct: 181 EKMHFVGFYNCWNDFLKDSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWY 240

Query: 241 EIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAV 300
           EIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAV
Sbjct: 241 EIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAV 300

Query: 301 TPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL 360
           TPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL
Sbjct: 301 TPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL 360

Query: 361 LGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL 420
           LGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL
Sbjct: 361 LGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL 420

Query: 421 YYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLR 480
           YYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLR
Sbjct: 421 YYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLR 480

Query: 481 VIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           VIGLALPGISAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 VIGLALPGISAHCLTNYIDSVRLGKKKNSL 510

[Close]

BLAST of Cp4.1LG03g14780 vs. NCBI nr
Match: XP_023526823.1 (uncharacterized protein LOC111790207 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 996 bits (2575), Expect = 0.0
Identity = 495/521 (95.01%), Postives = 495/521 (95.01%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG
Sbjct: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN
Sbjct: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180

Query: 181 EK--------------------------DSSTQVFILCDKPKDANLILISFRGTEPFDAD 240
           EK                          DSSTQVFILCDKPKDANLILISFRGTEPFDAD
Sbjct: 181 EKVIQNVVLRRWKMHFVGFYNCWNDFLKDSSTQVFILCDKPKDANLILISFRGTEPFDAD 240

Query: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLH 300
           DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLH
Sbjct: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLH 300

Query: 301 PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV 360
           PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV
Sbjct: 301 PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV 360

Query: 361 LHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDK 420
           LHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDK
Sbjct: 361 LHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDK 420

Query: 421 AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAE 480
           AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAE
Sbjct: 421 AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAE 480

Query: 481 YREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           YREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 YREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 521

[Close]

BLAST of Cp4.1LG03g14780 vs. NCBI nr
Match: KAG6581802.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 978 bits (2529), Expect = 0.0
Identity = 483/510 (94.71%), Postives = 489/510 (95.88%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATTRNNAGNDGDDF YLVVRPEKGGIRDVFRCW WPDGDGGRRFFEGSE GVFGAMVLG
Sbjct: 1   MATTRNNAGNDGDDFSYLVVRPEKGGIRDVFRCWVWPDGDGGRRFFEGSEEGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRL+MGNRTLMDLCIMASKLSYEN
Sbjct: 121 TETFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLQMGNRTLMDLCIMASKLSYEN 180

Query: 181 EK---------------DSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWY 240
           EK               DSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWY
Sbjct: 181 EKMHFVGFYNCWNDFLKDSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWY 240

Query: 241 EIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAV 300
           EIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISS+LSNIEQDRLHPSL+SEQFDAV
Sbjct: 241 EIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSNLSNIEQDRLHPSLNSEQFDAV 300

Query: 301 TPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL 360
           TPEMVQLSAYYAVK KLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL
Sbjct: 301 TPEMVQLSAYYAVKHKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL 360

Query: 361 LGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL 420
           LGVYTFGQPR+GDKQLAQFMEAHLMKP  RYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL
Sbjct: 361 LGVYTFGQPRVGDKQLAQFMEAHLMKPTTRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL 420

Query: 421 YYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLR 480
           YYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSL+MGYTYGAEYREGWFCLLLR
Sbjct: 421 YYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLVMGYTYGAEYREGWFCLLLR 480

Query: 481 VIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           VIGLALPGISAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 VIGLALPGISAHCLTNYIDSVRLGKKKNSL 510

[Close]

BLAST of Cp4.1LG03g14780 vs. NCBI nr
Match: XP_022955873.1 (uncharacterized protein LOC111457726 isoform X1 [Cucurbita moschata])

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 485/521 (93.09%), Postives = 490/521 (94.05%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATTRNNAGNDGDDF YLVVRPEKGGIRDVFRCW WPDGDGGRRFFEGSE GVFGAMVLG
Sbjct: 1   MATTRNNAGNDGDDFSYLVVRPEKGGIRDVFRCWVWPDGDGGRRFFEGSEEGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN
Sbjct: 121 TETFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180

Query: 181 EK--------------------------DSSTQVFILCDKPKDANLILISFRGTEPFDAD 240
           EK                          DSSTQVFILCDKPKDANLILISFRGTEPFDAD
Sbjct: 181 EKVIQNVVLRRWKMHFVGFYNCWNDFLKDSSTQVFILCDKPKDANLILISFRGTEPFDAD 240

Query: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLH 300
           DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQ RLH
Sbjct: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQVRLH 300

Query: 301 PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV 360
           PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV
Sbjct: 301 PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV 360

Query: 361 LHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDK 420
           LHKEMEIMDRLLGVYTFGQPR+G+KQLAQFMEAHLMKP PRYFRVVYCNDLVPRLPYDDK
Sbjct: 361 LHKEMEIMDRLLGVYTFGQPRVGNKQLAQFMEAHLMKPNPRYFRVVYCNDLVPRLPYDDK 420

Query: 421 AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAE 480
           AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSL+MGYTYGAE
Sbjct: 421 AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLVMGYTYGAE 480

Query: 481 YREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           YREGWFCLL+RVIGLALPGISAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 YREGWFCLLVRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 521

[Close]

BLAST of Cp4.1LG03g14780 vs. NCBI nr
Match: XP_022955874.1 (uncharacterized protein LOC111457726 isoform X2 [Cucurbita moschata])

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 480/501 (95.81%), Postives = 486/501 (97.01%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATTRNNAGNDGDDF YLVVRPEKGGIRDVFRCW WPDGDGGRRFFEGSE GVFGAMVLG
Sbjct: 1   MATTRNNAGNDGDDFSYLVVRPEKGGIRDVFRCWVWPDGDGGRRFFEGSEEGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN
Sbjct: 121 TETFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180

Query: 181 EKDSST------QVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIH 240
           EK          +VFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIH
Sbjct: 181 EKVIQNVVLRRWKVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIH 240

Query: 241 IGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAVTPEMVQLSA 300
           IGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQ RLHPSLDSEQFDAVTPEMVQLSA
Sbjct: 241 IGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQVRLHPSLDSEQFDAVTPEMVQLSA 300

Query: 301 YYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQP 360
           YYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQP
Sbjct: 301 YYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQP 360

Query: 361 RIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTEN 420
           R+G+KQLAQFMEAHLMKP PRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTEN
Sbjct: 361 RVGNKQLAQFMEAHLMKPNPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTEN 420

Query: 421 EVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLRVIGLALPGI 480
           EVDEESNKNFFGIRYLIPEYLNALWELIRSL+MGYTYGAEYREGWFCLL+RVIGLALPGI
Sbjct: 421 EVDEESNKNFFGIRYLIPEYLNALWELIRSLVMGYTYGAEYREGWFCLLVRVIGLALPGI 480

Query: 481 SAHCLTNYIDSVRLGKKKNSL 495
           SAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 SAHCLTNYIDSVRLGKKKNSL 501

[Close]

BLAST of Cp4.1LG03g14780 vs. ExPASy TrEMBL
Match: A0A6J1GV82 (uncharacterized protein LOC111457726 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457726 PE=4 SV=1)

HSP 1 Score: 978 bits (2527), Expect = 0.0
Identity = 485/521 (93.09%), Postives = 490/521 (94.05%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATTRNNAGNDGDDF YLVVRPEKGGIRDVFRCW WPDGDGGRRFFEGSE GVFGAMVLG
Sbjct: 1   MATTRNNAGNDGDDFSYLVVRPEKGGIRDVFRCWVWPDGDGGRRFFEGSEEGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN
Sbjct: 121 TETFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180

Query: 181 EK--------------------------DSSTQVFILCDKPKDANLILISFRGTEPFDAD 240
           EK                          DSSTQVFILCDKPKDANLILISFRGTEPFDAD
Sbjct: 181 EKVIQNVVLRRWKMHFVGFYNCWNDFLKDSSTQVFILCDKPKDANLILISFRGTEPFDAD 240

Query: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLH 300
           DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQ RLH
Sbjct: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQVRLH 300

Query: 301 PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV 360
           PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV
Sbjct: 301 PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV 360

Query: 361 LHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDK 420
           LHKEMEIMDRLLGVYTFGQPR+G+KQLAQFMEAHLMKP PRYFRVVYCNDLVPRLPYDDK
Sbjct: 361 LHKEMEIMDRLLGVYTFGQPRVGNKQLAQFMEAHLMKPNPRYFRVVYCNDLVPRLPYDDK 420

Query: 421 AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAE 480
           AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSL+MGYTYGAE
Sbjct: 421 AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLVMGYTYGAE 480

Query: 481 YREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           YREGWFCLL+RVIGLALPGISAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 YREGWFCLLVRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 521

[Close]

BLAST of Cp4.1LG03g14780 vs. ExPASy TrEMBL
Match: A0A6J1GXI2 (uncharacterized protein LOC111457726 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457726 PE=4 SV=1)

HSP 1 Score: 974 bits (2519), Expect = 0.0
Identity = 480/501 (95.81%), Postives = 486/501 (97.01%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATTRNNAGNDGDDF YLVVRPEKGGIRDVFRCW WPDGDGGRRFFEGSE GVFGAMVLG
Sbjct: 1   MATTRNNAGNDGDDFSYLVVRPEKGGIRDVFRCWVWPDGDGGRRFFEGSEEGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN
Sbjct: 121 TETFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180

Query: 181 EKDSST------QVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIH 240
           EK          +VFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIH
Sbjct: 181 EKVIQNVVLRRWKVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIH 240

Query: 241 IGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAVTPEMVQLSA 300
           IGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQ RLHPSLDSEQFDAVTPEMVQLSA
Sbjct: 241 IGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQVRLHPSLDSEQFDAVTPEMVQLSA 300

Query: 301 YYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQP 360
           YYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQP
Sbjct: 301 YYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVYTFGQP 360

Query: 361 RIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTEN 420
           R+G+KQLAQFMEAHLMKP PRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTEN
Sbjct: 361 RVGNKQLAQFMEAHLMKPNPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDSLFTEN 420

Query: 421 EVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLRVIGLALPGI 480
           EVDEESNKNFFGIRYLIPEYLNALWELIRSL+MGYTYGAEYREGWFCLL+RVIGLALPGI
Sbjct: 421 EVDEESNKNFFGIRYLIPEYLNALWELIRSLVMGYTYGAEYREGWFCLLVRVIGLALPGI 480

Query: 481 SAHCLTNYIDSVRLGKKKNSL 495
           SAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 SAHCLTNYIDSVRLGKKKNSL 501

[Close]

BLAST of Cp4.1LG03g14780 vs. ExPASy TrEMBL
Match: A0A6J1IU04 (uncharacterized protein LOC111479916 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479916 PE=4 SV=1)

HSP 1 Score: 952 bits (2460), Expect = 0.0
Identity = 476/530 (89.81%), Postives = 484/530 (91.32%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           M TTRNNAG+DGDDF YLVVRPEKGGIRDVFRCW WPDGDGGRRFFEG E GVFGAMVLG
Sbjct: 1   MVTTRNNAGSDGDDFSYLVVRPEKGGIRDVFRCWFWPDGDGGRRFFEGFEEGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKP+EWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPVEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFISTIGLLDGRMNLLNEEQILEGATDSVYEERGF L+MGNRTLMDLCIMASKLSYEN
Sbjct: 121 TETFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFGLKMGNRTLMDLCIMASKLSYEN 180

Query: 181 EK--------------------------DSSTQVFILCDKPKDANLILISFRGTEPFDAD 240
           EK                          DSSTQVFILCDK KDANLILISFRGTEPFDAD
Sbjct: 181 EKVIQNVVLRRWKMHFVGFYNCWNDFLKDSSTQVFILCDKLKDANLILISFRGTEPFDAD 240

Query: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLS-------- 300
           DWCTDFDYSWYEIP+VGKIHIGFLEALGLGNRNDANSFKGHHK  SISSD+S        
Sbjct: 241 DWCTDFDYSWYEIPDVGKIHIGFLEALGLGNRNDANSFKGHHKFISISSDVSRSTISNLH 300

Query: 301 -NIEQDRLHPSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGAL 360
            +IEQDRLHPSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGAL
Sbjct: 301 QSIEQDRLHPSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGAL 360

Query: 361 AVLFPTVLVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDL 420
           AVLFPTVLVLHKEMEIMDRLLGVYTFGQPR+GDKQLAQFMEAHLMKP PRYFRVVYCNDL
Sbjct: 361 AVLFPTVLVLHKEMEIMDRLLGVYTFGQPRVGDKQLAQFMEAHLMKPTPRYFRVVYCNDL 420

Query: 421 VPRLPYDDKAFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSL 480
           VPRLPYDDKAFFYKHFGVCLYYDSLFTE EVDEESNKNFFGIRYLIPEYLNALWELIRSL
Sbjct: 421 VPRLPYDDKAFFYKHFGVCLYYDSLFTEKEVDEESNKNFFGIRYLIPEYLNALWELIRSL 480

Query: 481 LMGYTYGAEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           LMGYTYGAEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 LMGYTYGAEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 530

[Close]

BLAST of Cp4.1LG03g14780 vs. ExPASy TrEMBL
Match: A0A6J1IRR6 (uncharacterized protein LOC111479916 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479916 PE=4 SV=1)

HSP 1 Score: 949 bits (2452), Expect = 0.0
Identity = 471/510 (92.35%), Postives = 480/510 (94.12%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           M TTRNNAG+DGDDF YLVVRPEKGGIRDVFRCW WPDGDGGRRFFEG E GVFGAMVLG
Sbjct: 1   MVTTRNNAGSDGDDFSYLVVRPEKGGIRDVFRCWFWPDGDGGRRFFEGFEEGVFGAMVLG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
           RRWVIVVSILIRKVIAALAKP+EWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG
Sbjct: 61  RRWVIVVSILIRKVIAALAKPVEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFISTIGLLDGRMNLLNEEQILEGATDSVYEERGF L+MGNRTLMDLCIMASKLSYEN
Sbjct: 121 TETFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFGLKMGNRTLMDLCIMASKLSYEN 180

Query: 181 EKDSST------QVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPEVGKIH 240
           EK          +VFILCDK KDANLILISFRGTEPFDADDWCTDFDYSWYEIP+VGKIH
Sbjct: 181 EKVIQNVVLRRWKVFILCDKLKDANLILISFRGTEPFDADDWCTDFDYSWYEIPDVGKIH 240

Query: 241 IGFLEALGLGNRNDANSFKGHHKLSSISSDLS---------NIEQDRLHPSLDSEQFDAV 300
           IGFLEALGLGNRNDANSFKGHHK  SISSD+S         +IEQDRLHPSLDSEQFDAV
Sbjct: 241 IGFLEALGLGNRNDANSFKGHHKFISISSDVSRSTISNLHQSIEQDRLHPSLDSEQFDAV 300

Query: 301 TPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL 360
           TPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL
Sbjct: 301 TPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRL 360

Query: 361 LGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL 420
           LGVYTFGQPR+GDKQLAQFMEAHLMKP PRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL
Sbjct: 361 LGVYTFGQPRVGDKQLAQFMEAHLMKPTPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCL 420

Query: 421 YYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLR 480
           YYDSLFTE EVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLR
Sbjct: 421 YYDSLFTEKEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLR 480

Query: 481 VIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           VIGLALPGISAHCLTNYIDSVRLGKKKNSL
Sbjct: 481 VIGLALPGISAHCLTNYIDSVRLGKKKNSL 510

[Close]

BLAST of Cp4.1LG03g14780 vs. ExPASy TrEMBL
Match: A0A1S3CKY2 (uncharacterized protein LOC103501974 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501974 PE=4 SV=1)

HSP 1 Score: 798 bits (2062), Expect = 4.66e-288
Identity = 399/533 (74.86%), Postives = 441/533 (82.74%), Query Frame = 0


Query: 1   MATTRNNAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLG 60
           MATT+     +GDDFRYLVVRPEKGGIRDVFRCW WPDGDGGRRFFE SE GVF A V G
Sbjct: 1   MATTQLK---NGDDFRYLVVRPEKGGIRDVFRCWVWPDGDGGRRFFESSEEGVFDAKVSG 60

Query: 61  RRWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRG 120
            RW+IVVS+LIRKVIA L KP+EWTGNVVEF+LNLLSMN N+ GL CNIVRGEVVVP+RG
Sbjct: 61  SRWIIVVSVLIRKVIAILGKPLEWTGNVVEFTLNLLSMNANLLGLLCNIVRGEVVVPRRG 120

Query: 121 TDTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN 180
           T+TFIST+GLLDGRM+LLNEE++L+G TD V EERG  LEMGNR L+DLC+MASKLSYEN
Sbjct: 121 TETFISTVGLLDGRMDLLNEEKLLKGTTDFVSEERGLGLEMGNRYLVDLCVMASKLSYEN 180

Query: 181 EK--------------------------DSSTQVFILCDKPKDANLILISFRGTEPFDAD 240
           EK                          D+ TQVFILC+KPKDANLILISFRGTEPFDAD
Sbjct: 181 EKVIQNIVLRYWKMHFVGFYNCWNDFQKDNCTQVFILCNKPKDANLILISFRGTEPFDAD 240

Query: 241 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKL-SSISSDLSNIEQDRL 300
           DW TDFDYSWYEIPEVGKIHIGFLEALGLGNRND NSF GH +  +SISS  S++     
Sbjct: 241 DWSTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDTNSFNGHLQAKTSISSIASDVSHGST 300

Query: 301 HP---------SLDS--EQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLG 360
            P         +LD   E FD VTPE+ QL+AYY VKL+LRRLLMEH+NAKFVVTGHSLG
Sbjct: 301 SPFGHTKSTISNLDQNIEHFDEVTPEVEQLTAYYTVKLQLRRLLMEHRNAKFVVTGHSLG 360

Query: 361 GALAVLFPTVLVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYC 420
           GALA+LFPTVLVLHKEMEIM RLLGVYTFGQPR+G+KQL QFME +L+ PIPRYFRVVYC
Sbjct: 361 GALAILFPTVLVLHKEMEIMGRLLGVYTFGQPRVGNKQLGQFMEPYLVNPIPRYFRVVYC 420

Query: 421 NDLVPRLPYDDKAFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELI 480
           ND+VPRLPYD+KAF +KHFGVCLYYDSLF E++VDEE NKNF GIRYLIPEYLNALWEL+
Sbjct: 421 NDIVPRLPYDNKAFLFKHFGVCLYYDSLFIEHKVDEEPNKNFLGIRYLIPEYLNALWELL 480

Query: 481 RSLLMGYTYGAEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLGKKKNSL 495
           RSLLMGYT+G EY+EGWFC+L+RVIGLA PGISAHCLTNYIDSVRLGKK  SL
Sbjct: 481 RSLLMGYTHGPEYKEGWFCILVRVIGLAFPGISAHCLTNYIDSVRLGKKSQSL 530

[Close]

BLAST of Cp4.1LG03g14780 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 531.6 bits (1368), Expect = 6.9e-151
Identity = 270/517 (52.22%), Postives = 358/517 (69.25%), Query Frame = 0


Query: 7   NAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVL-----GR 66
           +  N G    YL+VRP +GG  D+FR     D     +F E  +   +  + +       
Sbjct: 9   SGSNPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDH 68

Query: 67  RWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGT 126
           RWVIVVSIL+RK+I  L  PME+TG VV+F LNL S NG   GL   +++ +VV+P+RG+
Sbjct: 69  RWVIVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGS 128

Query: 127 DTFISTIGLLDGRMNLLNEEQILE--GATDSVYEERGFRLEMGNRTLMDLCIMASKLSYE 186
            TF+STIG LDGR++L  E   +E     DSV   R  ++E+G+R LMDLC+MASKL+YE
Sbjct: 129 VTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGR-VKIELGSRGLMDLCVMASKLAYE 188

Query: 187 N--------------------------EKDSSTQVFILCDKPKDANLILISFRGTEPFDA 246
           N                          +K  STQVF+  DK KDANLI+ISFRGTEPFDA
Sbjct: 189 NAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDA 248

Query: 247 DDWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKL-SSISSDLSNIEQDR 306
           DDW TDFDYSWYE+P VGK+H+GFLEA+GLGNR+D  +F  H+ L    SS+  N +++ 
Sbjct: 249 DDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTF--HYNLFEQTSSEEENSKKNL 308

Query: 307 LHPSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTV 366
           L              +MV+ SAYYAV++ L+RLL EH+NA+FVVTGHSLGGALA+LFPT+
Sbjct: 309 L--------------DMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTL 368

Query: 367 LVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYD 426
           LVL++E EIM RLLGVYTFGQPRIG++++  FM+A L +P+ RYFRVVYCND+VPRLPYD
Sbjct: 369 LVLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYD 428

Query: 427 DKAFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYG 486
           DK F YKHFG+CL+YDS + E + ++E + N +G+RY I  ++ A+WEL+R L MGYT+G
Sbjct: 429 DKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHG 488

Query: 487 AEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLG 490
            +Y+EGWF +L R++GL +PG+S HC+T+Y++SVRLG
Sbjct: 489 PDYKEGWFRILFRLMGLVIPGLSDHCMTDYVNSVRLG 508

[Close]

BLAST of Cp4.1LG03g14780 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 362.5 bits (929), Expect = 5.6e-100
Identity = 207/505 (40.99%), Postives = 283/505 (56.04%), Query Frame = 0


Query: 17  YLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMV-LGRRWVIVVSILIRKVI 76
           Y V+ P +  + D+     +    G R+F   SE  +   +    RRW+I VSI+I+K+I
Sbjct: 7   YFVLDPREATVSDLIHL-LFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQKLI 66

Query: 77  AALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGTDTFISTIGLLDGRM 136
               KP+ + G  +   LNLLS NG    +  N+ +G ++ P++ + TF S  G LD R+
Sbjct: 67  ILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDRRV 126

Query: 137 NLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN--------------- 196
            L            +   ERG       R    L IMASKLSYEN               
Sbjct: 127 EL------------NPKVERG-----SKRYKAMLSIMASKLSYENINFVSSVLHNHWKMD 186

Query: 197 -----------EKDSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPE 256
                      +K  ST+V ++ D   D NLI++SFRGT+PFDADDWCTD D SWYE+  
Sbjct: 187 LLGFYSCWNGYQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKN 246

Query: 257 VGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAVTPEM 316
           VGKIH GF++ALGL         +G  K                  +LD  Q +A T   
Sbjct: 247 VGKIHGGFMKALGLQK-------EGWPK----------------EVNLDETQ-NATT--- 306

Query: 317 VQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVY 376
             L AYY V+  L+ +L ++  +KF++TGHSLGGALA+LF  VLV+H E ++++RL GVY
Sbjct: 307 --LYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFTAVLVMHDEEQMLERLEGVY 366

Query: 377 TFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDS 436
           TFGQPR+GD++   FM+  L K   +Y R VYCND+VPRLP+DDK   +KHFG CLYYDS
Sbjct: 367 TFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLPFDDKTLMFKHFGACLYYDS 426

Query: 437 LFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLRVIGL 495
            +     +EE NKN+F + +++P+ +NALWELIRS +M Y  G E+REGWF    RV+ L
Sbjct: 427 FYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYWKGGEFREGWFLRCFRVVAL 464

[Close]

BLAST of Cp4.1LG03g14780 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 338.6 bits (867), Expect = 8.6e-93
Identity = 186/500 (37.20%), Postives = 282/500 (56.40%), Query Frame = 0


Query: 17  YLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLGR-RWVIVVSILIRKVI 76
           Y V+ P +  + D+ R   + D +  R+F + S   +   +   R RW+I VSI+++K++
Sbjct: 7   YFVLDPREATVTDLMRLLFYSDLE-NRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKLL 66

Query: 77  AALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGTDTFISTIGLLDGRM 136
             L KP+ + G  + F LNL S NG    +F N+V+G  + P++ + TF S  G LD ++
Sbjct: 67  IILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQKV 126

Query: 137 NLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYENE-------------- 196
                 ++  G +  + +ER   L         L IMASKL+YENE              
Sbjct: 127 ------ELGLGRSIKIGDERYKPL---------LSIMASKLAYENEHFIRSVLQDHWQMD 186

Query: 197 ------------KDSSTQVFILCDKPKDANLILISFRGTEPFDADDWCTDFDYSWYEIPE 256
                       +  ST+V ++ D   + NLI++SFRGT+PF+ADDWCTD D SW+ +  
Sbjct: 187 LLGFYSCPNDFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMN 246

Query: 257 VGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHPSLDSEQFDAVTPEM 316
           VGKIH GF++ALGL                          ++  +  ++S+Q    T ++
Sbjct: 247 VGKIHGGFMKALGL-------------------------PKEGWYEEINSDQTQNKTSQL 306

Query: 317 VQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLVLHKEMEIMDRLLGVY 376
               AYY +  +L+ +  ++  +KF+++GHSLGGALA+LF  VL++H E E+++RL GVY
Sbjct: 307 ----AYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILFTAVLIMHDEKEMLERLEGVY 366

Query: 377 TFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDKAFFYKHFGVCLYYDS 436
           TFGQPR+GD+    +M+  L +   +Y R VYCND+VPRLP+DDK   +KHFG CLY DS
Sbjct: 367 TFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRLPFDDKTLMFKHFGGCLYCDS 426

Query: 437 LFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAEYREGWFCLLLRVIGL 490
            +     +EE NKN+F I ++IP+ +NA+WELIRS ++    G EYREGW     R++ L
Sbjct: 427 FYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISCWQGREYREGWLLTCFRLVAL 461

[Close]

BLAST of Cp4.1LG03g14780 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 311.6 bits (797), Expect = 1.1e-84
Identity = 186/514 (36.19%), Postives = 279/514 (54.28%), Query Frame = 0


Query: 7   NAGNDGDDFRYLVVRPEKGGIRDVFRCWAWPDGDGGRRFFEGSEVGVFGAMVLGRRWVIV 66
           N+ +D +   YL++RPE+    ++ R     D +  R   + SE           RW+I 
Sbjct: 3   NSDDDDEPRGYLILRPEELRPWELVRLLFSGDIEKPRS-VDSSETEEHS---FRHRWLIF 62

Query: 67  VSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGTDTFIS 126
           VS+++ K++   +K +   G+ +EFSLN LS N + SGLF   +RGEVV+PQR ++ + S
Sbjct: 63  VSLVLLKLLRFFSKLLALVGSALEFSLNFLS-NNSFSGLF---LRGEVVMPQRTSENYQS 122

Query: 127 TIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN------ 186
            IG LD R++L                +     E G +    L IMASK++YEN      
Sbjct: 123 FIGHLDTRVSL----------------DMTLNREDGEKYYAALSIMASKIAYENAARIKH 182

Query: 187 --------------------EKDSSTQVFILCDKPK------DANLILISFRGTEPFDAD 246
                               ++  +TQ FI+               ++++FRGTE F+++
Sbjct: 183 VVENHWNMKYLGLVDYWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSE 242

Query: 247 DWCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLH 306
           DWC+DFD +W+E+P +G IH GF++ALGL N             S     LSN       
Sbjct: 243 DWCSDFDITWFELPNIGNIHGGFMKALGLQN-----------NCSWPKEPLSN------- 302

Query: 307 PSLDSEQFDAVTPEMVQLSAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTVLV 366
                       P+     AYY+++  L+ L+ ++KN KFV+TGHSLGGALA+LF  VLV
Sbjct: 303 ------------PDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGGALAILFTAVLV 362

Query: 367 LHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYDDK 426
           +H E E+++R+ GVYT+GQPR+GD +  +FME  L K   +Y+R VY ND+VPRLPYDDK
Sbjct: 363 IHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNNDIVPRLPYDDK 422

Query: 427 AFFYKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTYGAE 486
              +KHFG C+YYD  +    + E+S++NFF +R +I    +A+ E IRS  +    G+E
Sbjct: 423 DLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIRSFTIVAEKGSE 462

Query: 487 YREGWFCLLLRVIGLALPGISAHCLTNYIDSVRL 489
           Y EGW     R +G+ +PG+S H   +Y+++ RL
Sbjct: 483 YSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRL 462

[Close]

BLAST of Cp4.1LG03g14780 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 306.6 bits (784), Expect = 3.6e-83
Identity = 170/458 (37.12%), Postives = 248/458 (54.15%), Query Frame = 0


Query: 62  RWVIVVSILIRKVIAALAKPMEWTGNVVEFSLNLLSMNGNISGLFCNIVRGEVVVPQRGT 121
           RW++ ++I ++KV+  L+KP  + G  + + LNLL+ NG    L  N++ G++V P + +
Sbjct: 57  RWILALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSS 116

Query: 122 DTFISTIGLLDGRMNLLNEEQILEGATDSVYEERGFRLEMGNRTLMDLCIMASKLSYEN- 181
            T+ S IG  D R+ L  +E+I  G+ +  Y+               L IMASK+SYE+ 
Sbjct: 117 ATYTSFIGCSDRRIEL--DEKINVGSIE--YKSM-------------LSIMASKISYESK 176

Query: 182 -------------------------EKDSSTQVFILCDKPKDANLILISFRGTEPFDADD 241
                                    ++   TQ F+      + +LI++SFRGTEPF+A D
Sbjct: 177 PYITSVVKNTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAAD 236

Query: 242 WCTDFDYSWYEIPEVGKIHIGFLEALGLGNRNDANSFKGHHKLSSISSDLSNIEQDRLHP 301
           WCTD D SWYE+  VGK+H GF  ALGL                                
Sbjct: 237 WCTDLDLSWYEMKNVGKVHAGFSRALGL-------------------------------- 296

Query: 302 SLDSEQFDAVTPEMVQL---SAYYAVKLKLRRLLMEHKNAKFVVTGHSLGGALAVLFPTV 361
                Q D    E + L    AYY ++  LR  L  +KN K+++TGHSLGGALA LFP +
Sbjct: 297 -----QKDGWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPAI 356

Query: 362 LVLHKEMEIMDRLLGVYTFGQPRIGDKQLAQFMEAHLMKPIPRYFRVVYCNDLVPRLPYD 421
           L +H E E++D+L G+YTFGQPR+GD+   +FM+  + K    Y R VY ND+VPR+P+D
Sbjct: 357 LAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPFD 416

Query: 422 DKAFF-YKHFGVCLYYDSLFTENEVDEESNKNFFGIRYLIPEYLNALWELIRSLLMGYTY 481
           DK  F YKH+G C  ++SL+     ++  N N+F + +LIP+ L  LWE IRS ++ +  
Sbjct: 417 DKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFWK 460

Query: 482 GAEYREGWFCLLLRVIGLALPGISAHCLTNYIDSVRLG 490
           G EY+E W    +RV+G+  PG S H   +Y++S RLG
Sbjct: 477 GDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLG 460

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g14780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1S3ZP85	1.4e-156	51.64	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
F4JFU8	9.7e-150	52.22	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	9.8e-110	42.47	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	4.1e-07	32.04	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
P61871	1.6e-06	36.73	Lipase OS=Rhizopus niveus OX=4844 PE=1 SV=1	[more]

BLAST of Cp4.1LG03g14780 vs. NCBI nr


Match Name	Identity	Description
XP_023526824.1	97.06	uncharacterized protein LOC111790207 isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_023526823.1	95.01	uncharacterized protein LOC111790207 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6581802.1	94.71	Lipase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022955873.1	93.09	uncharacterized protein LOC111457726 isoform X1 [Cucurbita moschata]	[more]
XP_022955874.1	95.81	uncharacterized protein LOC111457726 isoform X2 [Cucurbita moschata]	[more]

BLAST of Cp4.1LG03g14780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GV82	0.0	93.09	uncharacterized protein LOC111457726 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GXI2	0.0	95.81	uncharacterized protein LOC111457726 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IU04	0.0	89.81	uncharacterized protein LOC111479916 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1IRR6	0.0	92.35	uncharacterized protein LOC111479916 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A1S3CKY2	4.66e-288	74.86	uncharacterized protein LOC103501974 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Cp4.1LG03g14780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14360.1	6.9e-151	52.22	alpha/beta-Hydrolases superfamily protein	[more]
AT5G42930.1	5.6e-100	40.99	alpha/beta-Hydrolases superfamily protein	[more]
AT1G56630.1	8.6e-93	37.20	alpha/beta-Hydrolases superfamily protein	[more]
AT5G67050.1	1.1e-84	36.19	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.1	3.6e-83	37.12	triacylglycerol lipase-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 203..393 e-value: 3.5E-43 score: 146.9
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 112..437 e-value: 3.1E-49 score: 170.2
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 174..413
None	No IPR available	PANTHER	PTHR46086:SF22	SUBFAMILY NOT NAMED	coord: 181..254 coord: 13..182 coord: 286..493
None	No IPR available	CDD	cd00519	Lipase_3	coord: 182..408 e-value: 2.48506E-42 score: 148.393
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 181..254 coord: 13..182
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 286..493

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g14780.1	Cp4.1LG03g14780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity