Cp4.1LG03g12920 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g12920
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionWRKY DNA-binding protein 75
LocationCp4.1LG03: 9956281 .. 9957935 (+)
RNA-Seq ExpressionCp4.1LG03g12920
SyntenyCp4.1LG03g12920
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGGAAAATTTCCCAAATTTTCTGACGACTCCATCTTCTTCAACCTCCCTCTCTCTGCAGCAAATGCTGATTTCTCGAGCTTCCGAGCTCGCCGGAATCCATGGAGGAGTCTCCGGCGGGGTTCCTCTGTTGTCGGATGGAGTAGATTTGTCGGAGAAGAAGGTGGGTGTGCCGGCGGCCGGACGGGGAGGTTATGAACAGAAAAAGAAGACGTTGAGCCGGAGATTCTGTTTTCAAACAAGAAGTCAAGTTGATATTCTTGATGATGGTTATAAATGGAGAAAGTATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGGTATTTATTATTATTATTTTTTTAATTTTAGAACTCAACATCGAACCCGATTAAGAGTGAGAGTGGGTGCGACGTAAAAACTAAGAGTATACACGAGTTGAGTTGGGCTAACTTAAAATTTTGAATTAGTTAGGTTAGTAATCCTCTGTGATTGGGTTGGGTCGAGTCGGGTCGAACTATCAGGTTAAAAAAACAAAGTTTAGACTAGTTATTCTAATGGGTTGGTTGGGCATGCGACTAAGAGTGAGAGTGGGTGCGAAAATCCACCCCACCACCAGCAGATATTGTCCTCTTTGGATTTTTCCTTTCAAACTTTTCTAAAACATGTATATTAGGGAGAAGTTTCCACACTTTTATAAAGAATGTTTCATTATCCTCTCTAACCAATGCGGGATCTCAAGCGGTGTGTTGACGCTGAGCGACGAGCAGCGTGCGAATGAACAAGATCGAGAATAGGTTAAATATTTTTTGAATTAGTTAGGTTAGTAATCCTCTGTGTTTGAATTGGGTTGGGTCGAACTATATCAGGTTATAAAACAAAATTTAGACCGGTTATTCTAATGGGTTGGTTGGGCATGCGACTAACAGTGAGAGTGGGTGCGAAAATCCACGCCCATCACTAGCAGATATTATCCTTTTTAGACTTTTCCTTTCAAACTTTTCTAAAACATGTATGCTAGGGAGAAGTTTCCACACTTTTATAAAGAATGTTTCATTATCCTCTCTAACCAATGCGGGATCTCAAGCGGTGTGTTGACGCTGAGCGACGAGCAGCGTGCGAATGAACAAGATCGAGAATAGGTTAAATTTTTTTTGAATTAGTTAGGTTAGTAATCCTCTGTGTTTGGATTGGGTTGGGTTGAGTTGGGTCGAGCTATCAGGTTAAAAAAAAGGTTTAGACTAGTTATTCTAATGAGTTGGTTAGGCATGCGACAAGAGTGAGAATGGGTGCATGGTGTGCGTTTGAGCGTGACACACGTAGTGATAGTGCGGTGGTGCAGGCTTGACAACATTGGTGTGGCGGCACTGAGAAAAAAAACCCTAAAGTGGAAGGTTAAAGTTAGTCATCTAGGTTGGGTTGATCGGTTTTTTTCGAGTTTTTGGATCGAGTATATACCCATCGATTGATTAAACTCTGAATAAAATTGCAGAAGCTACTACAGATGTACACACCAAGGGTGCAAAGTGAAGAAGCAAGTGCAAAGGCTAACTAGAGATGAAGGGGTGGTGGTCACAACTTATGAAGGCATCCACTCTCATCCCATTCTCAAATCCGCTGATAACTTCGAGTATATTTTGAGTCGCATGCAAATTTACGCTTCTTATTGA

mRNA sequence

ATGATGGAAAATTTCCCAAATTTTCTGACGACTCCATCTTCTTCAACCTCCCTCTCTCTGCAGCAAATGCTGATTTCTCGAGCTTCCGAGCTCGCCGGAATCCATGGAGGAGTCTCCGGCGGGGTTCCTCTGTTGTCGGATGGAGTAGATTTGTCGGAGAAGAAGGTGGGTGTGCCGGCGGCCGGACGGGGAGGTTATGAACAGAAAAAGAAGACGTTGAGCCGGAGATTCTGTTTTCAAACAAGAAGTCAAGTTGATATTCTTGATGATGGTTATAAATGGAGAAAGTATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGAAGCTACTACAGATGTACACACCAAGGGTGCAAAGTGAAGAAGCAAGTGCAAAGGCTAACTAGAGATGAAGGGGTGGTGGTCACAACTTATGAAGGCATCCACTCTCATCCCATTCTCAAATCCGCTGATAACTTCGAGTATATTTTGAGTCGCATGCAAATTTACGCTTCTTATTGA

Coding sequence (CDS)

ATGATGGAAAATTTCCCAAATTTTCTGACGACTCCATCTTCTTCAACCTCCCTCTCTCTGCAGCAAATGCTGATTTCTCGAGCTTCCGAGCTCGCCGGAATCCATGGAGGAGTCTCCGGCGGGGTTCCTCTGTTGTCGGATGGAGTAGATTTGTCGGAGAAGAAGGTGGGTGTGCCGGCGGCCGGACGGGGAGGTTATGAACAGAAAAAGAAGACGTTGAGCCGGAGATTCTGTTTTCAAACAAGAAGTCAAGTTGATATTCTTGATGATGGTTATAAATGGAGAAAGTATGGACAAAAGGCTGTGAAGAACAACAAATTCCCAAGAAGCTACTACAGATGTACACACCAAGGGTGCAAAGTGAAGAAGCAAGTGCAAAGGCTAACTAGAGATGAAGGGGTGGTGGTCACAACTTATGAAGGCATCCACTCTCATCCCATTCTCAAATCCGCTGATAACTTCGAGTATATTTTGAGTCGCATGCAAATTTACGCTTCTTATTGA

Protein sequence

MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGGVSGGVPLLSDGVDLSEKKVGVPAAGRGGYEQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY
Homology
BLAST of Cp4.1LG03g12920 vs. ExPASy Swiss-Prot
Match: Q9FYA2 (Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 PE=1 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 5.6e-41
Identity = 83/125 (66.40%), Postives = 97/125 (77.60%), Query Frame = 0

Query: 43  PLLSDGVDLSEKKVGVPAAGRGGYEQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAV 102
           P L  G + S  KV      +     KKK   +R+ FQTRSQVDILDDGY+WRKYGQKAV
Sbjct: 22  PELHQGEEES-SKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAV 81

Query: 103 KNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQ 162
           KNNKFPRSYYRCT+ GC VKKQVQRLT D+ VVVTTYEG+HSHPI KS +NFE+IL++MQ
Sbjct: 82  KNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQ 141

Query: 163 IYASY 168
           IY+S+
Sbjct: 142 IYSSF 145

BLAST of Cp4.1LG03g12920 vs. ExPASy Swiss-Prot
Match: Q9S763 (Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 9.2e-36
Identity = 67/101 (66.34%), Postives = 84/101 (83.17%), Query Frame = 0

Query: 67  EQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQ 126
           ++KKK    R+ FQTRSQVDILDDGY+WRKYGQKAVKNN FPRSYY+CT +GC+VKKQVQ
Sbjct: 43  KKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEEGCRVKKQVQ 102

Query: 127 RLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 168
           R   DEGVVVTTY+G+H+H + K +DNF +IL++M I+  +
Sbjct: 103 RQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIFPPF 143

BLAST of Cp4.1LG03g12920 vs. ExPASy Swiss-Prot
Match: Q9FFS3 (Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 7.1e-28
Identity = 59/108 (54.63%), Postives = 75/108 (69.44%), Query Frame = 0

Query: 62  GRGGYEQKKKTLSR--RFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGC 121
           G  G E K+K   +  R  F TRS  D+LDDGY+WRKYGQK+VK+N  PRSYYRCT+  C
Sbjct: 69  GEKGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTC 128

Query: 122 KVKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 168
            VKKQVQRL +D  VVVTTYEG+H+HP  K  +    +L ++Q  +S+
Sbjct: 129 NVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSSF 176

BLAST of Cp4.1LG03g12920 vs. ExPASy Swiss-Prot
Match: Q8VWQ4 (Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.6e-27
Identity = 72/152 (47.37%), Postives = 94/152 (61.84%), Query Frame = 0

Query: 12  PSSSTSLSLQQMLISRASELAGIHGGVSGGVPLLSDGVDLSEKKVGVPAAGRGGYEQKKK 71
           PSSS+       L ++     G  GG  GG  L+S+  + S+        G+G     K+
Sbjct: 45  PSSSSCTPAGYHLSAQLENFRG-GGGEMGG--LVSNNSNNSDHNKNC-NKGKG-----KR 104

Query: 72  TLS-RRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTR 131
           TL+ +R  F TRS  D+LDDGY+WRKYGQK+VKNN  PRSYYRCT+  C VKKQVQRL +
Sbjct: 105 TLAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQVQRLAK 164

Query: 132 DEGVVVTTYEGIHSHPILKSADNFEYILSRMQ 163
           D  VVVTTYEG+H+HP  K  +    +L ++Q
Sbjct: 165 DPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

BLAST of Cp4.1LG03g12920 vs. ExPASy Swiss-Prot
Match: Q93WY4 (Probable WRKY transcription factor 12 OS=Arabidopsis thaliana OX=3702 GN=WRKY12 PE=1 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.3e-26
Identity = 73/164 (44.51%), Postives = 93/164 (56.71%), Query Frame = 0

Query: 7   NFLT--TPSSSTSLSLQQMLISRASELA------GIHGGVSGGVPLLSDGVDLSEKKVGV 66
           N LT  + SSS+SLS     I  +S         G    + GG PL S  V+  ++  G 
Sbjct: 38  NVLTFFSSSSSSSLSSPSFPIHNSSSTTTTHAPLGFSNNLQGGGPLGSKVVNDDQENFGG 97

Query: 67  PA-------------AGRGGYEQK----KKTLSRRFCFQTRSQVDILDDGYKWRKYGQKA 126
                          +G G  + K    +K    RFCFQT+S VD+LDDGYKWRKYGQK 
Sbjct: 98  GTNNDAHSNSWWRSNSGSGDMKNKVKIRRKLREPRFCFQTKSDVDVLDDGYKWRKYGQKV 157

Query: 127 VKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 146
           VKN+  PRSYYRCTH  C+VKK+V+RL+ D  +V+TTYEG H+H
Sbjct: 158 VKNSLHPRSYYRCTHNNCRVKKRVERLSEDCRMVITTYEGRHNH 201

BLAST of Cp4.1LG03g12920 vs. NCBI nr
Match: XP_022956055.1 (probable WRKY transcription factor 75 [Cucurbita moschata] >KAG6582031.1 putative WRKY transcription factor 75, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018457.1 putative WRKY transcription factor 75, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 327 bits (838), Expect = 9.85e-113
Identity = 164/167 (98.20%), Postives = 164/167 (98.20%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGGVSGGVPLLSDGVDLSEKKVGVPA 60
           MMENFPNF TTPSSSTSLSLQQMLISRASELAGIHGGVSGGV LLSDGVDLSEKKVGVPA
Sbjct: 1   MMENFPNFPTTPSSSTSLSLQQMLISRASELAGIHGGVSGGVTLLSDGVDLSEKKVGVPA 60

Query: 61  AGRGGYEQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120
           AGRGGYEQKKKTLSRRF FQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK
Sbjct: 61  AGRGGYEQKKKTLSRRFSFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120

Query: 121 VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 167
           VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY
Sbjct: 121 VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 167

BLAST of Cp4.1LG03g12920 vs. NCBI nr
Match: XP_022979950.1 (probable WRKY transcription factor 75 [Cucurbita maxima])

HSP 1 Score: 315 bits (806), Expect = 7.47e-108
Identity = 156/167 (93.41%), Postives = 160/167 (95.81%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGGVSGGVPLLSDGVDLSEKKVGVPA 60
           MMENFPNF TTPSSSTSLSLQ+MLISRASEL GIHGGVSGGVPLLSDGVDLSEKK GVPA
Sbjct: 1   MMENFPNFPTTPSSSTSLSLQKMLISRASELGGIHGGVSGGVPLLSDGVDLSEKKEGVPA 60

Query: 61  AGRGGYEQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120
           AG+GGYEQKKKT+SRRF FQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK
Sbjct: 61  AGQGGYEQKKKTMSRRFSFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120

Query: 121 VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 167
           VKKQVQRLTRDE VVVTTYEGIH+HPILK  DNFEYILSRMQIYASY
Sbjct: 121 VKKQVQRLTRDEEVVVTTYEGIHTHPILKPTDNFEYILSRMQIYASY 167

BLAST of Cp4.1LG03g12920 vs. NCBI nr
Match: XP_038902977.1 (probable WRKY transcription factor 50 [Benincasa hispida])

HSP 1 Score: 240 bits (613), Expect = 1.02e-77
Identity = 136/217 (62.67%), Postives = 146/217 (67.28%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGG--------VSGGVPLLSD----- 60
           MMENFPNFL+T SSS+SLS  QML SRASEL G HGG        VSGG PL  D     
Sbjct: 1   MMENFPNFLSTSSSSSSLSFHQMLFSRASELVGSHGGDHYRQPPPVSGGFPLSRDVLGDD 60

Query: 61  ------------------------------GVD-LSEKKVGVPAA------GRGGYEQKK 120
                                         GVD L +K+V    A      GRG +++KK
Sbjct: 61  MSFDASSSMKDDDVIVSLSKSFRVDESARIGVDGLEKKEVATAGATTTTGTGRGDHDKKK 120

Query: 121 KTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTR 167
           K  SRRF FQTRSQVDILDDGY+WRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTR
Sbjct: 121 KMRSRRFAFQTRSQVDILDDGYRWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTR 180

BLAST of Cp4.1LG03g12920 vs. NCBI nr
Match: XP_022932710.1 (probable WRKY transcription factor 75 [Cucurbita moschata])

HSP 1 Score: 234 bits (596), Expect = 2.32e-75
Identity = 128/202 (63.37%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHG------GVSGGVPLLSDGVDLSEK 60
           MME++P+FL+  SSSTSLS Q+MLISRASE  GIHG      G+SGG PL  D V  S  
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGSHFREAGISGGFPLFPD-VSGSYM 60

Query: 61  KVG-----------------------------VPAAGRGGYEQKKKTLSRRFCFQTRSQV 120
            VG                             + AA +G  ++KKKT SRRF FQTRSQV
Sbjct: 61  SVGTRSSMKEDMVSVSKGSAVEVVEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQV 120

Query: 121 DILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 167
           DILDDGY+WRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH
Sbjct: 121 DILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 180

BLAST of Cp4.1LG03g12920 vs. NCBI nr
Match: XP_023539084.1 (probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >AXF54192.1 WRKY1 [Cucurbita pepo])

HSP 1 Score: 231 bits (590), Expect = 1.89e-74
Identity = 127/202 (62.87%), Postives = 142/202 (70.30%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGG------VSGGVPLLSDGVDLSEK 60
           MME++P+FL+  SSSTSLS Q+MLISRASE  GIHG       +SGG PL  D V  S  
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGSQFREAAISGGFPLFPD-VSGSYM 60

Query: 61  KVG-----------------------------VPAAGRGGYEQKKKTLSRRFCFQTRSQV 120
            VG                             + AA +G  ++KKKT SRRF FQTRSQV
Sbjct: 61  SVGTRSSTKEDMVSVSKGSAVEVVEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQV 120

Query: 121 DILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 167
           DILDDGY+WRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH
Sbjct: 121 DILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 180

BLAST of Cp4.1LG03g12920 vs. ExPASy TrEMBL
Match: A0A6J1GWS5 (probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111457864 PE=4 SV=1)

HSP 1 Score: 327 bits (838), Expect = 4.77e-113
Identity = 164/167 (98.20%), Postives = 164/167 (98.20%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGGVSGGVPLLSDGVDLSEKKVGVPA 60
           MMENFPNF TTPSSSTSLSLQQMLISRASELAGIHGGVSGGV LLSDGVDLSEKKVGVPA
Sbjct: 1   MMENFPNFPTTPSSSTSLSLQQMLISRASELAGIHGGVSGGVTLLSDGVDLSEKKVGVPA 60

Query: 61  AGRGGYEQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120
           AGRGGYEQKKKTLSRRF FQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK
Sbjct: 61  AGRGGYEQKKKTLSRRFSFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120

Query: 121 VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 167
           VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY
Sbjct: 121 VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 167

BLAST of Cp4.1LG03g12920 vs. ExPASy TrEMBL
Match: A0A6J1IUS6 (probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC111479484 PE=4 SV=1)

HSP 1 Score: 315 bits (806), Expect = 3.61e-108
Identity = 156/167 (93.41%), Postives = 160/167 (95.81%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGGVSGGVPLLSDGVDLSEKKVGVPA 60
           MMENFPNF TTPSSSTSLSLQ+MLISRASEL GIHGGVSGGVPLLSDGVDLSEKK GVPA
Sbjct: 1   MMENFPNFPTTPSSSTSLSLQKMLISRASELGGIHGGVSGGVPLLSDGVDLSEKKEGVPA 60

Query: 61  AGRGGYEQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120
           AG+GGYEQKKKT+SRRF FQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK
Sbjct: 61  AGQGGYEQKKKTMSRRFSFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCK 120

Query: 121 VKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 167
           VKKQVQRLTRDE VVVTTYEGIH+HPILK  DNFEYILSRMQIYASY
Sbjct: 121 VKKQVQRLTRDEEVVVTTYEGIHTHPILKPTDNFEYILSRMQIYASY 167

BLAST of Cp4.1LG03g12920 vs. ExPASy TrEMBL
Match: A0A6J1F2W9 (probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439177 PE=4 SV=1)

HSP 1 Score: 234 bits (596), Expect = 1.12e-75
Identity = 128/202 (63.37%), Postives = 143/202 (70.79%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHG------GVSGGVPLLSDGVDLSEK 60
           MME++P+FL+  SSSTSLS Q+MLISRASE  GIHG      G+SGG PL  D V  S  
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGSHFREAGISGGFPLFPD-VSGSYM 60

Query: 61  KVG-----------------------------VPAAGRGGYEQKKKTLSRRFCFQTRSQV 120
            VG                             + AA +G  ++KKKT SRRF FQTRSQV
Sbjct: 61  SVGTRSSMKEDMVSVSKGSAVEVVEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQV 120

Query: 121 DILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 167
           DILDDGY+WRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH
Sbjct: 121 DILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 180

BLAST of Cp4.1LG03g12920 vs. ExPASy TrEMBL
Match: A0A345BTD5 (WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 231 bits (590), Expect = 9.17e-75
Identity = 127/202 (62.87%), Postives = 142/202 (70.30%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHGG------VSGGVPLLSDGVDLSEK 60
           MME++P+FL+  SSSTSLS Q+MLISRASE  GIHG       +SGG PL  D V  S  
Sbjct: 1   MMESYPDFLSASSSSTSLSFQKMLISRASEPTGIHGSQFREAAISGGFPLFPD-VSGSYM 60

Query: 61  KVG-----------------------------VPAAGRGGYEQKKKTLSRRFCFQTRSQV 120
            VG                             + AA +G  ++KKKT SRRF FQTRSQV
Sbjct: 61  SVGTRSSTKEDMVSVSKGSAVEVVEGGAETTEMTAARQGDNDKKKKTRSRRFAFQTRSQV 120

Query: 121 DILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 167
           DILDDGY+WRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH
Sbjct: 121 DILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 180

BLAST of Cp4.1LG03g12920 vs. ExPASy TrEMBL
Match: A0A6J1I419 (probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC111470780 PE=4 SV=1)

HSP 1 Score: 228 bits (581), Expect = 2.52e-73
Identity = 127/206 (61.65%), Postives = 142/206 (68.93%), Query Frame = 0

Query: 1   MMENFPNFLTTPSSSTSLSLQQMLISRASELAGIHG------GVSGGVPLLSD------G 60
           MME++P+FL+  SSS SLS Q+ LISRASE  GIHG      G+SGG PL  D       
Sbjct: 1   MMESYPDFLSASSSSNSLSFQKTLISRASEPTGIHGSHFREAGISGGFPLFPDVSRSYMS 60

Query: 61  VD----LSEKKVGVP-----------------------AAGRGGYEQKKKTLSRRFCFQT 120
           VD    + E  V V                        AA +G  ++KKKT SRRF FQT
Sbjct: 61  VDTRSSMKEDMVSVSKGFHADGSAGEVVEGGAETTEMAAARQGDNDKKKKTRSRRFAFQT 120

Query: 121 RSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEG 167
           RSQVDILDDGY+WRKYGQK VKNNKFPRSYY+CTHQGCKVKKQVQRLTRDEGVVVTTYEG
Sbjct: 121 RSQVDILDDGYRWRKYGQKGVKNNKFPRSYYKCTHQGCKVKKQVQRLTRDEGVVVTTYEG 180

BLAST of Cp4.1LG03g12920 vs. TAIR 10
Match: AT5G13080.1 (WRKY DNA-binding protein 75 )

HSP 1 Score: 168.7 bits (426), Expect = 4.0e-42
Identity = 83/125 (66.40%), Postives = 97/125 (77.60%), Query Frame = 0

Query: 43  PLLSDGVDLSEKKVGVPAAGRGGYEQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAV 102
           P L  G + S  KV      +     KKK   +R+ FQTRSQVDILDDGY+WRKYGQKAV
Sbjct: 22  PELHQGEEES-SKVRSEGCSKSVESSKKKGKKQRYAFQTRSQVDILDDGYRWRKYGQKAV 81

Query: 103 KNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQ 162
           KNNKFPRSYYRCT+ GC VKKQVQRLT D+ VVVTTYEG+HSHPI KS +NFE+IL++MQ
Sbjct: 82  KNNKFPRSYYRCTYGGCNVKKQVQRLTVDQEVVVTTYEGVHSHPIEKSTENFEHILTQMQ 141

Query: 163 IYASY 168
           IY+S+
Sbjct: 142 IYSSF 145

BLAST of Cp4.1LG03g12920 vs. TAIR 10
Match: AT3G01970.1 (WRKY DNA-binding protein 45 )

HSP 1 Score: 151.4 bits (381), Expect = 6.6e-37
Identity = 67/101 (66.34%), Postives = 84/101 (83.17%), Query Frame = 0

Query: 67  EQKKKTLSRRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQ 126
           ++KKK    R+ FQTRSQVDILDDGY+WRKYGQKAVKNN FPRSYY+CT +GC+VKKQVQ
Sbjct: 43  KKKKKEREARYAFQTRSQVDILDDGYRWRKYGQKAVKNNPFPRSYYKCTEEGCRVKKQVQ 102

Query: 127 RLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 168
           R   DEGVVVTTY+G+H+H + K +DNF +IL++M I+  +
Sbjct: 103 RQWGDEGVVVTTYQGVHTHAVDKPSDNFHHILTQMHIFPPF 143

BLAST of Cp4.1LG03g12920 vs. TAIR 10
Match: AT5G41570.1 (WRKY DNA-binding protein 24 )

HSP 1 Score: 125.2 bits (313), Expect = 5.0e-29
Identity = 59/108 (54.63%), Postives = 75/108 (69.44%), Query Frame = 0

Query: 62  GRGGYEQKKKTLSR--RFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGC 121
           G  G E K+K   +  R  F TRS  D+LDDGY+WRKYGQK+VK+N  PRSYYRCT+  C
Sbjct: 69  GEKGKELKEKRSRKVPRIAFHTRSDDDVLDDGYRWRKYGQKSVKHNAHPRSYYRCTYHTC 128

Query: 122 KVKKQVQRLTRDEGVVVTTYEGIHSHPILKSADNFEYILSRMQIYASY 168
            VKKQVQRL +D  VVVTTYEG+H+HP  K  +    +L ++Q  +S+
Sbjct: 129 NVKKQVQRLAKDPNVVVTTYEGVHNHPCEKLMETLNPLLRQLQFLSSF 176

BLAST of Cp4.1LG03g12920 vs. TAIR 10
Match: AT1G64000.1 (WRKY DNA-binding protein 56 )

HSP 1 Score: 124.0 bits (310), Expect = 1.1e-28
Identity = 72/152 (47.37%), Postives = 94/152 (61.84%), Query Frame = 0

Query: 12  PSSSTSLSLQQMLISRASELAGIHGGVSGGVPLLSDGVDLSEKKVGVPAAGRGGYEQKKK 71
           PSSS+       L ++     G  GG  GG  L+S+  + S+        G+G     K+
Sbjct: 45  PSSSSCTPAGYHLSAQLENFRG-GGGEMGG--LVSNNSNNSDHNKNC-NKGKG-----KR 104

Query: 72  TLS-RRFCFQTRSQVDILDDGYKWRKYGQKAVKNNKFPRSYYRCTHQGCKVKKQVQRLTR 131
           TL+ +R  F TRS  D+LDDGY+WRKYGQK+VKNN  PRSYYRCT+  C VKKQVQRL +
Sbjct: 105 TLAMQRIAFHTRSDDDVLDDGYRWRKYGQKSVKNNAHPRSYYRCTYHTCNVKKQVQRLAK 164

Query: 132 DEGVVVTTYEGIHSHPILKSADNFEYILSRMQ 163
           D  VVVTTYEG+H+HP  K  +    +L ++Q
Sbjct: 165 DPNVVVTTYEGVHNHPCEKLMETLSPLLRQLQ 187

BLAST of Cp4.1LG03g12920 vs. TAIR 10
Match: AT2G44745.1 (WRKY family transcription factor )

HSP 1 Score: 120.9 bits (302), Expect = 9.5e-28
Identity = 73/164 (44.51%), Postives = 93/164 (56.71%), Query Frame = 0

Query: 7   NFLT--TPSSSTSLSLQQMLISRASELA------GIHGGVSGGVPLLSDGVDLSEKKVGV 66
           N LT  + SSS+SLS     I  +S         G    + GG PL S  V+  ++  G 
Sbjct: 38  NVLTFFSSSSSSSLSSPSFPIHNSSSTTTTHAPLGFSNNLQGGGPLGSKVVNDDQENFGG 97

Query: 67  PA-------------AGRGGYEQK----KKTLSRRFCFQTRSQVDILDDGYKWRKYGQKA 126
                          +G G  + K    +K    RFCFQT+S VD+LDDGYKWRKYGQK 
Sbjct: 98  GTNNDAHSNSWWRSNSGSGDMKNKVKIRRKLREPRFCFQTKSDVDVLDDGYKWRKYGQKV 157

Query: 127 VKNNKFPRSYYRCTHQGCKVKKQVQRLTRDEGVVVTTYEGIHSH 146
           VKN+  PRSYYRCTH  C+VKK+V+RL+ D  +V+TTYEG H+H
Sbjct: 158 VKNSLHPRSYYRCTHNNCRVKKRVERLSEDCRMVITTYEGRHNH 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FYA25.6e-4166.40Probable WRKY transcription factor 75 OS=Arabidopsis thaliana OX=3702 GN=WRKY75 ... [more]
Q9S7639.2e-3666.34Probable WRKY transcription factor 45 OS=Arabidopsis thaliana OX=3702 GN=WRKY45 ... [more]
Q9FFS37.1e-2854.63Probable WRKY transcription factor 24 OS=Arabidopsis thaliana OX=3702 GN=WRKY24 ... [more]
Q8VWQ41.6e-2747.37Probable WRKY transcription factor 56 OS=Arabidopsis thaliana OX=3702 GN=WRKY56 ... [more]
Q93WY41.3e-2644.51Probable WRKY transcription factor 12 OS=Arabidopsis thaliana OX=3702 GN=WRKY12 ... [more]
Match NameE-valueIdentityDescription
XP_022956055.19.85e-11398.20probable WRKY transcription factor 75 [Cucurbita moschata] >KAG6582031.1 putativ... [more]
XP_022979950.17.47e-10893.41probable WRKY transcription factor 75 [Cucurbita maxima][more]
XP_038902977.11.02e-7762.67probable WRKY transcription factor 50 [Benincasa hispida][more]
XP_022932710.12.32e-7563.37probable WRKY transcription factor 75 [Cucurbita moschata][more]
XP_023539084.11.89e-7462.87probable WRKY transcription factor 75 isoform X1 [Cucurbita pepo subsp. pepo] >A... [more]
Match NameE-valueIdentityDescription
A0A6J1GWS54.77e-11398.20probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111457... [more]
A0A6J1IUS63.61e-10893.41probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC11147948... [more]
A0A6J1F2W91.12e-7563.37probable WRKY transcription factor 75 OS=Cucurbita moschata OX=3662 GN=LOC111439... [more]
A0A345BTD59.17e-7562.87WRKY1 OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1I4192.52e-7361.65probable WRKY transcription factor 75 OS=Cucurbita maxima OX=3661 GN=LOC11147078... [more]
Match NameE-valueIdentityDescription
AT5G13080.14.0e-4266.40WRKY DNA-binding protein 75 [more]
AT3G01970.16.6e-3766.34WRKY DNA-binding protein 45 [more]
AT5G41570.15.0e-2954.63WRKY DNA-binding protein 24 [more]
AT1G64000.11.1e-2847.37WRKY DNA-binding protein 56 [more]
AT2G44745.19.5e-2844.51WRKY family transcription factor [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 88..147
e-value: 1.0E-37
score: 141.3
IPR003657WRKY domainPFAMPF03106WRKYcoord: 89..146
e-value: 1.2E-25
score: 89.5
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 83..148
score: 29.241034
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 73..147
e-value: 1.6E-33
score: 116.3
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 80..147
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 67..164
NoneNo IPR availablePANTHERPTHR31221:SF83WRKY TRANSCRIPTION FACTOR 75-RELATEDcoord: 67..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g12920.1Cp4.1LG03g12920.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding