Cp4.1LG03g12730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g12730
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Ribonuclease
Location	Cp4.1LG03: 10065231 .. 10068944 (+)
RNA-Seq Expression	Cp4.1LG03g12730
Synteny	Cp4.1LG03g12730

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCGTAAAGAATTAGGGCCGATTGAGCGGGAAGCTGTGAGAGGTATCAGATAAAAGCTTCCATGGCGGGAATCGACCTAAAAGGAACACACAATCGAAAACGCTCTAGAGGGGAAGTGAGATTTGGTAGTAAACCGACGTAGTGAGAAGAACTCAGCAATGTCTTCGCAAGATCCACTCCCGAAATGGGCATCGAAGCCTTGTGTTATGGGCATCGACGAAGCTGGTCGAGGCCCTGTTTTAGGTTTTCTATTTTCCTATTCGTTTTCTTCCATTTCTGCATCTTTAACACCGCTTCGATTATCTTATTTTGGGATGTGATTTTGTGTATTTTGTTTCTGATATATTACTGCGTTTGATCTCAGGGCCAATGGTGTATGGATGCTTGTACTGTACTCGTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGGTTTAGCTTTTCTAAACGATTGATGCTTCTACCGATTTTATTTTAAGTGTTTTTCATCATTTTTCAGTTTCGTTTACTTTTCTTCCAATTTTTGATGTTTTGTTTTGCATCTAGACTCAAAAACATTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAGGTTTGAAGGCTAATGAGTCAATTGGATGGGCTGTTGATGTCATAGATCCTCGGGAACTTTCATCCAAAATGCTAAATAAGTCAGTATTTGGTGGTTTATAAATTCTTTCATTTGCACAAACACTCTAAGCTAACGGATAATGATTCTTTAACAACGGAAGGCAAAATGTTAAGTCCAACATAATGTATCATATGAGAACTTTCACGCCTGGGTTTTTTTCTGAAGTTTTTGTTTTTATTGTTCAACAGAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTTACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTAACTCTCTCCCTGGTTTCTTGCATTCATGAGTCAGTTATGCGATTTTAATATAGTATTTCTTTCAACAAGAAATGAAATTGCCGTTGATAGATGAAAAGAAATAGAAATTGTTAAGAACACACTCTCTCAACAGAGAAAGCAGAGGCAACAACTTGAGAAGATTGGATAGCCTTTGGTAACTTCCAATTATTTTTTGTATGCCTTTGTATATTCGTTCATGAGAGTTAGCAGGATGCGACATGTTCTTTATGAGGAAGATCTGTAAAAATTAAAATTTGGAAAGTTATATTCATGATTTATTCAGGTCCATAAGATAGAGGGAAAGAAAATCCCCATTCAGCATATCTCATCTTCCATTGCCTTCAAACTTTTTTGTTATCAATTGATGATTTGAAGTCATTTGCAGATTTGTTCTGCCCCAAGAACAATGTTAGATCAATCACTTCTATTTATTTTCAGGTTTATGTGGATACTGTTGGAGATCCTGAGAAGTACAGAATCAAGTTATCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAGGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTTGCAAAGGTAATGCTGATGCGTTCTTTTAAGATCTGGCATTCCAACCGTTTCCCACTCAAATTCATCACTTTGACTTGTAGGTCACAAGAGATCGTGTGTTGCGGGATTGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGGTTAATTTCATCCTCTAAATATTTTTTGCAGCATACTATAATCACGTTTTCACTTCACTGGGTGAAATCACTAGTGCTCTTATGATTTAATCTATGTAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACATTGTTGAAGTATCATGGTTAGTTTTTACTCTGCCTTGCAAGTATTATATTTCTTTGCAGAATTAGCTCATTATGCCCTTTTATGTTCTCCACGGTATAAAAAGGACAGGCTTTGTTGTTTAAAATGCTATATATTTTCTTTTAGCTCGTTATCCCCTATTGGACTAGGTATGTTGTTTGAAGTGCGTTTGTTAGTGATGGTGGGATTGATTTAATGAAAGCAGTCTTAAAAACAAGTTTGAATATCATATGGAGTTTAGTAATTAATGATGTACAATTGTTTATTGATGTAATGCACTTCGCATTCTTGCTGGATCATCATCCTGCTCTGATGTACTGAGATGTTCGGTTCTTTCCCATTCTAAATTAGAACTTGATTTGTTCACTGAAAATGAATGATTCTTCCTTTATTCTAGAATATAGTTTTGTTTGGTGCATCCCACTTTTATTGTACAATAGTTAGATATCCAAATAGTTTTGGTTCGTTCTTCGGGTCTACTTCGTGATACACCCACGTTCAGTGTTTTTTTAAACATTAGTGAGTGTTTAGACTCTAATCTCACAGGGCAAAACCCTGACATTTAGTTGTCAAGAAACTCACAAGATTGTAATATTCTAGGTAAGTGACCATGACCTCCATACAATTTGAACCCATTCTTCAAAATCCTTTGCGGCTTGGCCAACCTAGGATGGCTATGCCTAGTTTCTGTTCATCAATTTAGCTTCTCATAAATCATCAAATAAGCATAATATGATCCATTTGTTTACATATGAGCAGAAAATTTGTAACGGCCTAAGCCCACCGCCTTTTCTTCTTTGGGCTTTACTTTTCGGGCTTTTCCTCAAGGTTTATAAAACGCATCTGTTAAGGAGAGGTTTCCACACCCTTATAAAGAGTGTTTCGTTCTCCTCTCCAACCGATGTGAGATCACCCTCTTTGGGGCCTAGCGTCCTTGCTAGCACTCGTTCCTTTCTCCAATCAATGTTGGACCCCCACCAAATCCACCCCCTTCGAGGCCCAGCGTCCTTGCTGGCACATCGCCTCATGTCCACCCCCCTTTGGAACTCAGCCTCCTCACTGACACATCGCTCGATGTTCGGCTCTGATACCATTTGTAACGGCCCAAGTCCACTGCTAGTAGATATTGTCCTCTTTGGACTTTACTTTTCGGACTTCCCCACAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGGGTGTTTCATTCTCCTTCCCAACTGACGTGGGATTTCAAAGAATTAGACGCCGAAACTTCTCATACTTCCTTGACACTGACTCAAACTTCAAAGTGTTATGGAAGTTGTTTTGATTATGGAATCACTATGATGTAAGTTTTCTTTTCTCTATGTGCTTGATGTCCAGGGAGTCAGACAAAGTGGATGAAGATGGGGCAACGGGTGGAAGTGGTAAGAAACAATTAAGACTAAGCAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCGAAAGGACGGTGCAAATTTTTCCAGGCTAGAAAACTCGAACAACTCACTCACTTCTGAGAGTTCAATTTCATTCTATTGTTTCGACCATCCTCCTCTTTTTGATAATCAACTCAATAGCTGATAAGTGCTATTATCTGGTTTGGCTGTGTACTAATGTAGTTATAGATAACTAGCTATGGTACTGGCATCAATGTACATGACTTCCTCCATACCCATTTCAATGTCTGATAATTGTTTTGGAATTCTTGAAACGTTCTTGTTTATATACATTTTGAGAAGTGTTCTTAGTTCTCTCAC

mRNA sequence

TGCGTAAAGAATTAGGGCCGATTGAGCGGGAAGCTGTGAGAGGTATCAGATAAAAGCTTCCATGGCGGGAATCGACCTAAAAGGAACACACAATCGAAAACGCTCTAGAGGGGAAGTGAGATTTGGTAGTAAACCGACGTAGTGAGAAGAACTCAGCAATGTCTTCGCAAGATCCACTCCCGAAATGGGCATCGAAGCCTTGTGTTATGGGCATCGACGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCTTGTACTGTACTCGTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGACTCAAAAACATTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAGGTTTGAAGGCTAATGAGTCAATTGGATGGGCTGTTGATGTCATAGATCCTCGGGAACTTTCATCCAAAATGCTAAATAAAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTTACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTTTATGTGGATACTGTTGGAGATCCTGAGAAGTACAGAATCAAGTTATCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAGGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTTGCAAAGGTCACAAGAGATCGTGTGTTGCGGGATTGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACATTGTTGAAGTATCATGGGAGTCAGACAAAGTGGATGAAGATGGGGCAACGGGTGGAAGTGGTAAGAAACAATTAAGACTAAGCAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCGAAAGGACGGTGCAAATTTTTCCAGGCTAGAAAACTCGAACAACTCACTCACTTCTGAGAGTTCAATTTCATTCTATTGTTTCGACCATCCTCCTCTTTTTGATAATCAACTCAATAGCTGATAAGTGCTATTATCTGGTTTGGCTGTGTACTAATGTAGTTATAGATAACTAGCTATGGTACTGGCATCAATGTACATGACTTCCTCCATACCCATTTCAATGTCTGATAATTGTTTTGGAATTCTTGAAACGTTCTTGTTTATATACATTTTGAGAAGTGTTCTTAGTTCTCTCAC

Coding sequence (CDS)

ATGTCTTCGCAAGATCCACTCCCGAAATGGGCATCGAAGCCTTGTGTTATGGGCATCGACGAAGCTGGTCGAGGCCCTGTTTTAGGGCCAATGGTGTATGGATGCTTGTACTGTACTCGTTCCTATTTGAAAGAACTTTCGTCTTTGAATTTTGCAGACTCAAAAACATTGAAAGAAGAGAAGAGGGAGGAATTGTTTGAAGGTTTGAAGGCTAATGAGTCAATTGGATGGGCTGTTGATGTCATAGATCCTCGGGAACTTTCATCCAAAATGCTAAATAAAGTAAAGATAAACCTAAATGAAATATCACATGACTCTGCAATGGGTCTGGTTACCAAGGTTCTAGGCATGGGAGTTCTTTTAACTGAGGTTTATGTGGATACTGTTGGAGATCCTGAGAAGTACAGAATCAAGTTATCTGAAAAATTTCCCAATGTAAAATTTGTAGTTGCAAAGAAGGCTGATAGTTTATATCCTGTTGTCAGTGGGGCAAGCATAGTTGCAAAGGTCACAAGAGATCGTGTGTTGCGGGATTGGGCATTGGAGGAAACAGCTGAAAACATGCATAGAAACTTTGGTTCTGGATACCCTGGAGATCCAACAACTAAATGTTGGTTAGAAGATCACAAGCACTCGGTATTTGGGTTCCCCACATTGGTCCGCTTCAGTTGGGGTACCTGCACTTCATATTTCAAAGACATTGTTGAAGTATCATGGGAGTCAGACAAAGTGGATGAAGATGGGGCAACGGGTGGAAGTGGTAAGAAACAATTAAGACTAAGCAATGTTGGTATCACCACATCCAAGAGAAAGAGTGAAGAAATTGAATCAAATGCGAAAGGACGGTGCAAATTTTTCCAGGCTAGAAAACTCGAACAACTCACTCACTTCTGA

Protein sequence

MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF

Homology

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BLAST of Cp4.1LG03g12730 vs. ExPASy Swiss-Prot
Match: Q9SEZ6 (Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2)

HSP 1 Score: 444.9 bits (1143), Expect = 7.2e-124
Identity = 211/297 (71.04%), Postives = 256/297 (86.20%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREEL+E LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTE Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTRDR L++W 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK WL  HKHSVFGFP+LVRFSWGTCT++ K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           +D+ +E G  G S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLT F
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy Swiss-Prot
Match: Q5U209 (Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.3e-66
Identity = 120/245 (48.98%), Postives = 165/245 (67.35%), Query Frame = 0


Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 66  LFEGLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTE 125
           LF  ++ + + +GWA+D++ P  +S+ ML +VK NLN +SHD+A GL+   +   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDILSPNLISTSMLGRVKYNLNSMSHDTAAGLIQHAMDQNVKVTQ 138

Query: 126 VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEE 185
           V+VDTVG PE Y+ +L ++FP ++  V  KADSL+PVVS ASI+AKV RD+ +++W   E
Sbjct: 139 VFVDTVGMPETYQARLQQRFPGIEVTVKAKADSLFPVVSAASIIAKVARDQAVKNWQFVE 198

Query: 186 TAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYF-KDIVEVSWESD 245
           + + +  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  +   K+   V+WE  
Sbjct: 199 SLQGLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQAILEKEAESVTWEDS 258

Query: 246 KVDED 248
             +ED
Sbjct: 259 AAEED 263

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy Swiss-Prot
Match: Q9CWY8 (Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2)

HSP 1 Score: 252.7 bits (644), Expect = 5.2e-66
Identity = 122/245 (49.80%), Postives = 164/245 (66.94%), Query Frame = 0


Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC  S L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLADLEALKVADSKTLTENERER 78

Query: 66  LFEGLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTE 125
           LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L   V +T+
Sbjct: 79  LFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTAAGLIQYALDQNVNVTQ 138

Query: 126 VYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEE 185
           V+VDTVG PE Y+ +L + FP ++  V  KADSL+PVVS ASI AKV RD+ +++W   E
Sbjct: 139 VFVDTVGMPETYQARLQQHFPGIEVTVKAKADSLFPVVSAASIFAKVARDKAVKNWQFVE 198

Query: 186 TAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYF-KDIVEVSWESD 245
             +++  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  +   K+  +V WE  
Sbjct: 199 NLQDLDSDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWSTAQAILEKEAEDVIWEDS 258

Query: 246 KVDED 248
           + +ED
Sbjct: 259 EAEED 263

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy Swiss-Prot
Match: O75792 (Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2)

HSP 1 Score: 250.4 bits (638), Expect = 2.6e-65
Identity = 130/292 (44.52%), Postives = 180/292 (61.64%), Query Frame = 0


Query: 6   PLPKWASK-PCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEEKREE 65
           P+P    K PCV+G+DEAGRGPVLGPMVY   YC    L +L +L  ADSKTL E +RE 
Sbjct: 19  PVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERER 78

Query: 66  LFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVLLTEV 125
           LF  ++  + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GL+   L  GV +T+V
Sbjct: 79  LFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQV 138

Query: 126 YVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWALEET 185
           +VDTVG PE Y+ +L + FP ++  V  KAD+LYPVVS ASI AKV RD+ ++ W   E 
Sbjct: 139 FVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEK 198

Query: 186 AENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTC-TSYFKDIVEVSWESDK 245
            +++  ++GSGYP DP TK WL++H   VFGFP  VRFSW T  T   K+  +V WE   
Sbjct: 199 LQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWE--- 258

Query: 246 VDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT 296
             +  +    G +++        TS   +E  ++  +   ++F  R LE  T
Sbjct: 259 --DSASENQEGLRKI--------TSYFLNEGSQARPRSSHRYFLERGLESAT 297

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy Swiss-Prot
Match: Q2TBT5 (Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 1.7e-64
Identity = 135/297 (45.45%), Postives = 181/297 (60.94%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           +SS  P P    +PCV+G+DEAGRGPVLGPMVY   YC  S L++L +L  ADSKTL E 
Sbjct: 16  LSSPVP-PVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLEDLEALKVADSKTLSES 75

Query: 61  KREELFEGLKAN-ESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGV 120
           +R+ LF  ++ + + +GWA+DV+ P  +S+ ML +VK NLN +SHD+A GLV   L  GV
Sbjct: 76  ERDRLFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNALSHDTATGLVQFALDQGV 135

Query: 121 LLTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDW 180
            + +V+VDTVG PE Y+ +L ++FP ++  V  KAD+LYPVVS ASI AKV RD+ +++W
Sbjct: 136 NVAQVFVDTVGLPETYQERLQQRFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKNW 195

Query: 181 ALEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVE-VS 240
              E  +++  ++GSGYP DP TK WL  H   VFGFP  VRFSW T  S  +   E V 
Sbjct: 196 KFVEKLQDLDTDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWRTAQSILESEAEDVK 255

Query: 241 WESDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT 296
           WE     E G   G GK                SE  ++  +   ++FQ R LE  T
Sbjct: 256 WED---SETGDPKGPGK-----------IKSYFSESPQTCLRLPHRYFQERGLESAT 297

[Close]

BLAST of Cp4.1LG03g12730 vs. NCBI nr
Match: XP_023527419.1 (ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602 bits (1551), Expect = 4.21e-217
Identity = 297/297 (100.00%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. NCBI nr
Match: XP_022979887.1 (ribonuclease H2 subunit A [Cucurbita maxima])

HSP 1 Score: 599 bits (1544), Expect = 4.91e-216
Identity = 295/297 (99.33%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVV+KKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFP+LVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPSLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. NCBI nr
Match: XP_022955431.1 (ribonuclease H2 subunit A [Cucurbita moschata])

HSP 1 Score: 598 bits (1542), Expect = 9.92e-216
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. NCBI nr
Match: KAG6582048.1 (Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 597 bits (1539), Expect = 2.84e-215
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLT F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTQF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. NCBI nr
Match: XP_038902264.1 (ribonuclease H2 subunit A [Benincasa hispida])

HSP 1 Score: 578 bits (1491), Expect = 5.91e-208
Identity = 286/297 (96.30%), Postives = 291/297 (97.98%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQ P+PKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQYPVPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLG+GVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGIGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFP+VKFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPSVKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGAT GSGKKQLRLS+VGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATTGSGKKQLRLSSVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy TrEMBL
Match: A0A6J1IPY0 (Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1)

HSP 1 Score: 599 bits (1544), Expect = 2.38e-216
Identity = 295/297 (99.33%), Postives = 297/297 (100.00%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVV+KKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVSKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFP+LVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPSLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy TrEMBL
Match: A0A6J1GTR5 (Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1)

HSP 1 Score: 598 bits (1542), Expect = 4.80e-216
Identity = 295/297 (99.33%), Postives = 295/297 (99.33%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSK LKEE
Sbjct: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKALKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFEGLK NESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL
Sbjct: 61  KREELFEGLKTNESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy TrEMBL
Match: A0A5A7TT92 (Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 PE=3 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 3.34e-207
Identity = 284/297 (95.62%), Postives = 288/297 (96.97%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MS QD LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQL+ F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy TrEMBL
Match: A0A1S3BXP1 (Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1)

HSP 1 Score: 576 bits (1484), Expect = 3.34e-207
Identity = 284/297 (95.62%), Postives = 288/297 (96.97%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MS QD LPKWASKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLKELSSLNFADSKTLKEE
Sbjct: 1   MSLQDSLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKELSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISH SAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHGSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQL+ F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. ExPASy TrEMBL
Match: A0A0A0KTZ4 (Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1)

HSP 1 Score: 575 bits (1481), Expect = 9.57e-207
Identity = 282/297 (94.95%), Postives = 288/297 (96.97%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           MSSQD LPKW SKPCVMGIDEAGRGPVLGPMVYGCLYC  SYLK+LSSLNFADSKTLKEE
Sbjct: 1   MSSQDSLPKWTSKPCVMGIDEAGRGPVLGPMVYGCLYCASSYLKQLSSLNFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREELFE LKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVL MGVL
Sbjct: 61  KREELFEDLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLSMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTEVYVDTVGDPEKYRIKLSEKFPN+KFVVAKKADSLYPVVSGASIVAKVTRDR LR+WA
Sbjct: 121 LTEVYVDTVGDPEKYRIKLSEKFPNIKFVVAKKADSLYPVVSGASIVAKVTRDRALREWA 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKD+VEVSWE
Sbjct: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDVVEVSWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 297
           SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIES+ KGRCKFFQARKLEQL+ F
Sbjct: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESSGKGRCKFFQARKLEQLSRF 297

[Close]

BLAST of Cp4.1LG03g12730 vs. TAIR 10
Match: AT2G25100.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 444.9 bits (1143), Expect = 5.1e-125
Identity = 211/297 (71.04%), Postives = 256/297 (86.20%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREEL+E LK ++S+GWAVDVIDPRELS+KML K K NLNEISH+SAMGL+ +VL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKMLAKNKTNLNEISHNSAMGLIKRVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTE Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTRDR L++W 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK WL  HKHSVFGFP+LVRFSWGTCT++ K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           +D+ +E G  G S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLT F
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 296

[Close]

BLAST of Cp4.1LG03g12730 vs. TAIR 10
Match: AT2G25100.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 409.1 bits (1050), Expect = 3.1e-114
Identity = 198/297 (66.67%), Postives = 240/297 (80.81%), Query Frame = 0


Query: 1   MSSQDPLPKWASKPCVMGIDEAGRGPVLGPMVYGCLYCTRSYLKELSSLNFADSKTLKEE 60
           M S+   P+WAS+PC+MGIDEAGRGPVLGPMVYGC+YC  SY   L+SL+FADSKTLKEE
Sbjct: 1   MESECLTPEWASQPCLMGIDEAGRGPVLGPMVYGCMYCPISYQSSLASLHFADSKTLKEE 60

Query: 61  KREELFEGLKANESIGWAVDVIDPRELSSKMLNKVKINLNEISHDSAMGLVTKVLGMGVL 120
           KREEL+E LK ++S+GWAVDVIDPRELS+KML                    KVL MGVL
Sbjct: 61  KREELYESLKLDKSLGWAVDVIDPRELSAKML-------------------AKVLDMGVL 120

Query: 121 LTEVYVDTVGDPEKYRIKLSEKFPNVKFVVAKKADSLYPVVSGASIVAKVTRDRVLRDWA 180
           LTE Y+DTVGDP+KYRIKLSE+FP++KFVV+KKADSL+P+VSGASIVAKVTRDR L++W 
Sbjct: 121 LTEAYLDTVGDPDKYRIKLSERFPSIKFVVSKKADSLFPIVSGASIVAKVTRDRALKEWL 180

Query: 181 LEETAENMHRNFGSGYPGDPTTKCWLEDHKHSVFGFPTLVRFSWGTCTSYFKDIVEVSWE 240
           +EET E+++RNFGSGYPGDP TK WL  HKHSVFGFP+LVRFSWGTCT++ K  VEV+WE
Sbjct: 181 VEETGEDINRNFGSGYPGDPETKAWLVQHKHSVFGFPSLVRFSWGTCTTHLKGEVEVAWE 240

Query: 241 SDKVDEDGATGGSGKKQLRLSNVGITTSKRKSEEIESNAKGRCKFFQARKLEQLTHF 298
           +D+ +E G  G S K+Q +LS+ G  T +++SEEIES+ KGRCKF QARK++QLT F
Sbjct: 241 ADENEESG-NGSSSKRQAKLSSFGFKTCEKRSEEIESSGKGRCKFLQARKIQQLTQF 277

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g12730 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SEZ6	7.2e-124	71.04	Ribonuclease H2 subunit A OS=Arabidopsis thaliana OX=3702 GN=At2g25100 PE=2 SV=2	[more]
Q5U209	2.3e-66	48.98	Ribonuclease H2 subunit A OS=Rattus norvegicus OX=10116 GN=Rnaseh2a PE=2 SV=1	[more]
Q9CWY8	5.2e-66	49.80	Ribonuclease H2 subunit A OS=Mus musculus OX=10090 GN=Rnaseh2a PE=1 SV=2	[more]
O75792	2.6e-65	44.52	Ribonuclease H2 subunit A OS=Homo sapiens OX=9606 GN=RNASEH2A PE=1 SV=2	[more]
Q2TBT5	1.7e-64	45.45	Ribonuclease H2 subunit A OS=Bos taurus OX=9913 GN=RNASEH2A PE=1 SV=1	[more]

BLAST of Cp4.1LG03g12730 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527419.1	4.21e-217	100.00	ribonuclease H2 subunit A [Cucurbita pepo subsp. pepo]	[more]
XP_022979887.1	4.91e-216	99.33	ribonuclease H2 subunit A [Cucurbita maxima]	[more]
XP_022955431.1	9.92e-216	99.33	ribonuclease H2 subunit A [Cucurbita moschata]	[more]
KAG6582048.1	2.84e-215	99.33	Ribonuclease H2 subunit A, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038902264.1	5.91e-208	96.30	ribonuclease H2 subunit A [Benincasa hispida]	[more]

BLAST of Cp4.1LG03g12730 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IPY0	2.38e-216	99.33	Ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111479443 PE=3 SV=1	[more]
A0A6J1GTR5	4.80e-216	99.33	Ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111457459 PE=3 SV=1	[more]
A0A5A7TT92	3.34e-207	95.62	Ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G001310 ...	[more]
A0A1S3BXP1	3.34e-207	95.62	Ribonuclease OS=Cucumis melo OX=3656 GN=LOC103494542 PE=3 SV=1	[more]
A0A0A0KTZ4	9.57e-207	94.95	Ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_4G015800 PE=3 SV=1	[more]

BLAST of Cp4.1LG03g12730 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G25100.1	5.1e-125	71.04	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G25100.2	3.1e-114	66.67	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024567	Ribonuclease HII/HIII domain	PFAM	PF01351	RNase_HII	coord: 17..227 e-value: 3.8E-52 score: 177.0
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 2..186 e-value: 6.9E-67 score: 227.0
IPR004649	Ribonuclease H2, subunit A	TIGRFAM	TIGR00729	TIGR00729	coord: 16..228 e-value: 1.2E-54 score: 183.5
IPR023160	Ribonuclease HII, helix-loop-helix cap domain superfamily	GENE3D	1.10.10.460	Ribonuclease hii. Domain 2	coord: 187..235 e-value: 6.7E-20 score: 72.5
None	No IPR available	PANTHER	PTHR10954:SF19	RIBONUCLEASE	coord: 7..296
None	No IPR available	CDD	cd07181	RNase_HII_eukaryota_like	coord: 17..233 e-value: 5.8372E-139 score: 389.185
IPR001352	Ribonuclease HII/HIII	PANTHER	PTHR10954	RIBONUCLEASE H2 SUBUNIT A	coord: 7..296
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 15..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g12730.1	Cp4.1LG03g12730.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043137	DNA replication, removal of RNA primer
biological_process	GO:0006298	mismatch repair
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0016070	RNA metabolic process
cellular_component	GO:0032299	ribonuclease H2 complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0003676	nucleic acid binding