Cp4.1LG03g12320 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g12320 vs. ExPASy Swiss-Prot
Match: F4ISQ7 (DNA topoisomerase 3-beta OS=Arabidopsis thaliana OX=3702 GN=At2g32000 PE=2 SV=1)

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 660/863 (76.48%), Postives = 749/863 (86.79%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GSTEVHEFDG F G  AHY+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  PARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREGENICF 125
           P ++Q+W   DP DLF A +IK ESNPKAHI +HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF   D ++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 SFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIMQN 245
           +F+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI ++
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNLLKVAS 305
           GYEL L+WER RLFD + AT+FQK V+E   A+V  +SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYVRMLL 365
           SALG+GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE MVG DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGNTSPPD 485
           + SI GE F+CTG  V  KGFT+IMPW AV+EKKLP FLKG++IEV +V+LY+GNT+PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIEQQIT VAKGQADH  VVQ+V+QQF++K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCPLDNFE 665
           PLA+SGRALSKCGKCLRYMK I++ P RL+CGTCEEVYYLPQ+G +KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFLCPHPT 725
           L+I+S+PGPEGKSFPLCPYCYNSPPFEGIDTL+GA+KT  +   +  G GMPC LCPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNEKCAEC 785
           C+HS+  QGVCACPEC GTLVLDPV  P WKLNCN C+C+V LP+GAHRI TT+ +C EC
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE GATL  GC+LCDE L SL+E+KHG+SF R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGRGRGRSSGKLMDPKMSFRDF 869
           GRG   GR   K +DPKMSFRDF
Sbjct: 847 GRG---GRRGSKSVDPKMSFRDF 865

BLAST of Cp4.1LG03g12320 vs. ExPASy Swiss-Prot
Match: Q0J0S6 (DNA topoisomerase 3-beta OS=Oryza sativa subsp. japonica OX=39947 GN=TOP3B PE=2 SV=1)

HSP 1 Score: 1335.9 bits (3456), Expect = 0.0e+00
Identity = 630/868 (72.58%), Postives = 736/868 (84.79%), Query Frame = 0

Query: 2   VASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHVF 61
           +A +VLMVAEKPSIALSIASALS G+MSTRKGST+VHEFDG F G HA +KVTSVIGHV 
Sbjct: 1   MAPTVLMVAEKPSIALSIASALSGGRMSTRKGSTDVHEFDGMFQGSHAFFKVTSVIGHVL 60

Query: 62  SIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREGE 121
           S+DFP  +Q+W+ TDP+DLF A V+++E NPKAHIR+HL++EARGC  LVLWLDCDREGE
Sbjct: 61  SVDFPPAYQNWEGTDPMDLFVAPVLRSECNPKAHIRRHLAQEARGCTYLVLWLDCDREGE 120

Query: 122 NICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDL 181
           NIC+EVI+CTG   ++  ++++RA+FSSVTEKDI+ A++NLV P+++EALAVDARQEIDL
Sbjct: 121 NICYEVIDCTGIPKSEVGRRIFRAKFSSVTEKDIMDAMNNLVLPSKDEALAVDARQEIDL 180

Query: 182 KVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPY 241
           KVGV+FTRFQT +FQGKYGNLDSR+ISYGPCQTPTLGFCVQR+ QITTFKPEKFWS+  Y
Sbjct: 181 KVGVAFTRFQTRYFQGKYGNLDSRVISYGPCQTPTLGFCVQRYQQITTFKPEKFWSLKTY 240

Query: 242 IMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNLL 301
           ++++G E+ L+W+R +LFD DV  MFQK V  DGI +VT IS K+E K RP GLNTVNLL
Sbjct: 241 VIKDGNEIQLEWDRKKLFDFDVTVMFQKMVASDGILKVTDISVKEECKARPPGLNTVNLL 300

Query: 302 KVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYV 361
           KVASSALG GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF+  L +L  NP W + V
Sbjct: 301 KVASSALGIGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFRSALAALAHNPLWSNDV 360

Query: 362 RMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKYI 421
           R LL  G+ KP+ G D GDHPPITPM LATE+ +G DAWRLYQY+CQHFIG++S DC+Y 
Sbjct: 361 RTLLDTGFVKPKQGHDAGDHPPITPMRLATEEALGTDAWRLYQYICQHFIGTVSPDCRYT 420

Query: 422 RTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGNT 481
           RT I+F+  GE F+C G+ V  KGFTSIMPWLAV+E  +P + KG  + + KVD+Y+G+T
Sbjct: 421 RTSIEFTSGGETFHCVGNRVTSKGFTSIMPWLAVSENNIPAYKKGDAVSIHKVDIYEGST 480

Query: 482 SPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGY 541
           +PPDYLSESELISLMEK+GIGTDASIPVH+NNI ERNYVQV +GRRLVPT LG TLIRGY
Sbjct: 481 TPPDYLSESELISLMEKNGIGTDASIPVHVNNICERNYVQVNSGRRLVPTPLGTTLIRGY 540

Query: 542 QCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALFE 601
           QCID DLCLPDIR FIEQQIT +AKG+ADH  VVQ+VLQQF +KYSYFVKKI++MDALFE
Sbjct: 541 QCIDADLCLPDIRRFIEQQITLIAKGEADHLQVVQHVLQQFMKKYSYFVKKIENMDALFE 600

Query: 602 AQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCPL 661
           AQFSPLA+SGR LSKCGKC RYMK+IS+QP RLYC TCEEVYYLPQ G+IKLYKE+ CPL
Sbjct: 601 AQFSPLADSGRLLSKCGKCARYMKYISTQPMRLYCVTCEEVYYLPQNGSIKLYKEIICPL 660

Query: 662 DNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFLC 721
           D FELL+FS+ GP+ KSFPLCP+CYNSPPFEGID L+GA K +  +G   KG GMPCFLC
Sbjct: 661 DGFELLLFSMVGPDAKSFPLCPFCYNSPPFEGIDKLFGALKLD-DTGKVGKGAGMPCFLC 720

Query: 722 PHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNEK 781
            HPTC+ S+ITQGVCACPEC+GTL+LDPV AP W+L CN+CNC+V LP  AH+I+TT++K
Sbjct: 721 LHPTCKQSMITQGVCACPECTGTLILDPVSAPKWRLYCNRCNCIVLLPHAAHKISTTDKK 780

Query: 782 CAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGR 841
           C  C+STIIEVDFNKKTTPL+ GATL  GCILCDE LHSL+EMKHGKSFF  RG RGRGR
Sbjct: 781 CPTCESTIIEVDFNKKTTPLKDGATLHEGCILCDELLHSLIEMKHGKSFFMRRG-RGRGR 840

Query: 842 GRGRGRGRGRG-RSSGKLMDPKMSFRDF 869
           GRGRGRG  RG R S +  DPKMSFRDF
Sbjct: 841 GRGRGRGSSRGRRGSSRHDDPKMSFRDF 866

BLAST of Cp4.1LG03g12320 vs. ExPASy Swiss-Prot
Match: Q9Z321 (DNA topoisomerase 3-beta-1 OS=Mus musculus OX=10090 GN=Top3b PE=1 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 4.5e-267
Identity = 456/856 (53.27%), Postives = 588/856 (68.69%), Query Frame = 0

Query: 5   SVLMVAEKPSIALSIASALSHGQMSTRK---GSTEVHEFDGRFLGHHAHYKVTSVIGHVF 64
           +VLMVAEKPS+A SIA  LS G MS+ K   G+  VH++ G F G   H+K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGNMSSHKGLNGACSVHKYTGTFAGQPVHFKMTSVCGHVM 62

Query: 65  SIDFPARFQDWKATDPLDLF-QASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 124
           ++DF  ++  W   DP +LF QA   K E+NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYVVLWLDCDKEG 122

Query: 125 ENICFEVIEC---TGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQ 184
           ENICFEV++         ++  K V+RARFSS+T+ DI  A+  L EP+ NEAL+VDARQ
Sbjct: 123 ENICFEVLDAVLPVMNNAHNGEKTVFRARFSSITDTDICNAMTRLSEPDHNEALSVDARQ 182

Query: 185 EIDLKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWS 244
           E+DL++G +FTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE +W 
Sbjct: 183 ELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPETYWV 242

Query: 245 VCPYIMQNGYE-LNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLN 304
           +   +  +  E L LDW+R R+FD ++A MF      +  A V   S K+++K RP  LN
Sbjct: 243 LQAKVHTDKEESLLLDWDRVRVFDWEIAQMFLNMTKLEKEAWVEATSRKEKAKQRPLALN 302

Query: 305 TVNLLKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPE 364
           TV +L+VASSALG GPQ AMQ+AERLYTQG+ISYPRTE+T YP +FD KG+L     +P 
Sbjct: 303 TVEMLRVASSALGMGPQHAMQIAERLYTQGYISYPRTETTHYPENFDLKGSLRQQANHPY 362

Query: 365 WGDYVRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSS 424
           W D V+ LL++G ++PR G D GDHPPITPM  ATE  +G DAWRLY+Y+ +HFI ++S 
Sbjct: 363 WADSVKQLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIATVSH 422

Query: 425 DCKYIRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVN-EKKLPQFLKGQKIEVAKVD 484
           DCKY+++ I F I  E F C G +V+  GFT IMPW +V  E+ LP   KG    V +V 
Sbjct: 423 DCKYLQSTISFRIGPEHFTCMGKTVISPGFTEIMPWQSVPLEESLPTCQKGDTFTVGEVK 482

Query: 485 LYQGNTSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 544
           + +  TSPPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTNLGI
Sbjct: 483 MLEKQTSPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTNLGI 542

Query: 545 TLIRGYQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDH 604
            L+ GY  ID +L LP IRS +E+Q+  +A+G+AD+  V+ + L  FK+K+ YFV  I  
Sbjct: 543 VLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYHQVLGHTLDIFKRKFHYFVDSIAG 602

Query: 605 MDALFEAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYK 664
           MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ G IKLYK
Sbjct: 603 MDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIKLYK 662

Query: 665 ELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMG 724
           EL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 KGMG
Sbjct: 663 ELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KKGMG 722

Query: 725 MPCFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHR 784
             C  C HPTC+HSL   G+  C EC +G LVLDP   P WK+ CN CN +    + AHR
Sbjct: 723 --CNECTHPTCQHSLSMLGIGQCVECENGVLVLDPTSGPKWKVACNTCNVVAHCFENAHR 782

Query: 785 IATTNEKCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFF-RG 844
           +  + + C  C++ +++VDFNK  +PL G  T   GCI CD     L+E+KH  S     
Sbjct: 783 VRVSADTCNTCEAALLDVDFNKAKSPLPGNETQHTGCIFCDPVFQELVELKHAASCHPMH 839

Query: 845 RGSRGRGRGRGRGRGR 850
           RG  GR +GRGRGRGR
Sbjct: 843 RGGPGRRQGRGRGRGR 839

BLAST of Cp4.1LG03g12320 vs. ExPASy Swiss-Prot
Match: O95985 (DNA topoisomerase 3-beta-1 OS=Homo sapiens OX=9606 GN=TOP3B PE=1 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 3.2e-265
Identity = 451/859 (52.50%), Postives = 589/859 (68.57%), Query Frame = 0

Query: 5   SVLMVAEKPSIALSIASALSHGQMSTRK---GSTEVHEFDGRFLGHHAHYKVTSVIGHVF 64
           +VLMVAEKPS+A SIA  LS G +S+ K   G+  VHE+ G F G    +K+TSV GHV 
Sbjct: 3   TVLMVAEKPSLAQSIAKILSRGSLSSHKGLNGACSVHEYTGTFAGQPVRFKMTSVCGHVM 62

Query: 65  SIDFPARFQDWKATDPLDLF-QASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 124
           ++DF  ++  W   DP +LF QA   K E+NPK ++ + L  E RGCD +VLWLDCD+EG
Sbjct: 63  TLDFLGKYNKWDKVDPAELFSQAPTEKKEANPKLNMVKFLQVEGRGCDYIVLWLDCDKEG 122

Query: 125 ENICFEVIECT------GFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVD 184
           ENICFEV++          GG    K V+RARFSS+T+ DI  A+  L EP+ NEAL+VD
Sbjct: 123 ENICFEVLDAVLPVMNKAHGG---EKTVFRARFSSITDTDICNAMACLGEPDHNEALSVD 182

Query: 185 ARQEIDLKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEK 244
           ARQE+DL++G +FTRFQT +FQGKYG+LDS LIS+GPCQTPTLGFCV+RH +I +FKPE 
Sbjct: 183 ARQELDLRIGCAFTRFQTKYFQGKYGDLDSSLISFGPCQTPTLGFCVERHDKIQSFKPET 242

Query: 245 FWSVCPYI-MQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPS 304
           +W +   +       L LDW+R R+FD ++A MF      +  A+V   S K+++K RP 
Sbjct: 243 YWVLQAKVNTDKDRSLLLDWDRVRVFDREIAQMFLNMTKLEKEAQVEATSRKEKAKQRPL 302

Query: 305 GLNTVNLLKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVK 364
            LNTV +L+VASS+LG GPQ AMQ AERLYTQG+ISYPRTE+T YP +FD KG+L     
Sbjct: 303 ALNTVEMLRVASSSLGMGPQHAMQTAERLYTQGYISYPRTETTHYPENFDLKGSLRQQAN 362

Query: 365 NPEWGDYVRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGS 424
           +P W D V+ LL++G ++PR G D GDHPPITPM  ATE  +G DAWRLY+Y+ +HFI +
Sbjct: 363 HPYWADTVKRLLAEGINRPRKGHDAGDHPPITPMKSATEAELGGDAWRLYEYITRHFIAT 422

Query: 425 LSSDCKYIRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVN-EKKLPQFLKGQKIEVA 484
           +S DCKY+++ I F I  E+F C+G +V+  GFT +MPW +V  E+ LP   +G    V 
Sbjct: 423 VSHDCKYLQSTISFRIGPELFTCSGKTVLSPGFTEVMPWQSVPLEESLPTCQRGDAFPVG 482

Query: 485 KVDLYQGNTSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTN 544
           +V + +  T+PPDYL+E+ELI+LMEKHGIGTDASIPVHINNI +RNYV V++GRRL PTN
Sbjct: 483 EVKMLEKQTNPPDYLTEAELITLMEKHGIGTDASIPVHINNICQRNYVTVESGRRLKPTN 542

Query: 545 LGITLIRGYQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKK 604
           LGI L+ GY  ID +L LP IRS +E+Q+  +A+G+AD+  V+ + L  FK+K+ YFV  
Sbjct: 543 LGIVLVHGYYKIDAELVLPTIRSAVEKQLNLIAQGKADYRQVLGHTLDVFKRKFHYFVDS 602

Query: 605 IDHMDALFEAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIK 664
           I  MD L E  FSPLA +G+ LS+CGKC R+MK+I ++P RL+C  C+E Y LPQ G IK
Sbjct: 603 IAGMDELMEVSFSPLAATGKPLSRCGKCHRFMKYIQAKPSRLHCSHCDETYTLPQNGTIK 662

Query: 665 LYKELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSK 724
           LYKEL CPLD+FEL+++S  G  GKS+PLCPYCYN PPF  +                 K
Sbjct: 663 LYKELRCPLDDFELVLWS-SGSRGKSYPLCPYCYNHPPFRDM----------------KK 722

Query: 725 GMGMPCFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVGAPNWKLNCNKCNCLVFLPQG 784
           GMG  C  C HP+C+HSL   G+  C EC SG LVLDP   P WK+ CNKCN +    + 
Sbjct: 723 GMG--CNECTHPSCQHSLSMLGIGQCVECESGVLVLDPTSGPKWKVACNKCNVVAHCFEN 782

Query: 785 AHRIATTNEKCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFF 844
           AHR+  + + C+ C++ +++VDFNK  +PL G  T  +GC+ CD     L+E+KH  S  
Sbjct: 783 AHRVRVSADTCSVCEAALLDVDFNKAKSPLPGDETQHMGCVFCDPVFQELVELKHAASCH 839

Query: 845 -RGRGSRGRGRGRGRGRGR 850
              RG  GR +GRGRGR R
Sbjct: 843 PMHRGGPGRRQGRGRGRAR 839

BLAST of Cp4.1LG03g12320 vs. ExPASy Swiss-Prot
Match: O96651 (DNA topoisomerase 3-beta OS=Drosophila melanogaster OX=7227 GN=Top3beta PE=2 SV=2)

HSP 1 Score: 830.5 bits (2144), Expect = 1.8e-239
Identity = 421/864 (48.73%), Postives = 563/864 (65.16%), Query Frame = 0

Query: 5   SVLMVAEKPSIALSIASALSHGQMSTRKGS---TEVHEFDGRFLGH-HAHYKVTSVIGHV 64
           SVLMVAEKPS+A S+A  LS+G+ + ++G+      HE+ G F      H+++TSV GHV
Sbjct: 3   SVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGNFRNEGSVHFRMTSVCGHV 62

Query: 65  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 124
            S+DF  ++  W   DP+ LF  +  K E+NPK ++R+ L+ EARGCD LVLWLDCD+EG
Sbjct: 63  MSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFLAHEARGCDYLVLWLDCDKEG 122

Query: 125 ENICFEVIECTGFG-GNDYRKKV-YRARFSSVTEKDILKALDNLVEPNRNEALAVDARQE 184
           ENICFEV++       N Y  +V YRA FS++TEKDI KA++ L  PN NEA +VDARQE
Sbjct: 123 ENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIKKAMETLGHPNENEAKSVDARQE 182

Query: 185 IDLKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSV 244
           +DL++G +FTRFQT FFQ +YG+LDS LISYGPCQTPTLGFCV+RH  I TFKPE FW +
Sbjct: 183 LDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQTPTLGFCVKRHDDIQTFKPESFWHL 242

Query: 245 CPYIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTV 304
              ++    E+ L+W RGR+F  D+A M   RV E   A V  ++ K+  K +P  LNTV
Sbjct: 243 --QLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEHKKATVESVASKEAYKSKPQALNTV 302

Query: 305 NLLKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWG 364
            L+++ SS LG GP  AMQ+AERLYTQG+ISYPRTE+  YP++FD    LH L  + ++G
Sbjct: 303 ELMRICSSGLGIGPFQAMQIAERLYTQGYISYPRTETNQYPTNFDLPAVLHVLKPSADFG 362

Query: 365 DYVRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDC 424
           +  R +L D    PR G D GDHPPITPM L       RD WR+Y+++C+HF+G++S D 
Sbjct: 363 EEARSILGD-IQTPRKGKDAGDHPPITPMKLGNRSDFDRDTWRVYEFICRHFMGTVSRDL 422

Query: 425 KYIRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAV-NEKKLPQFLKGQKIEVAKVDLY 484
           KY  T  + S+  E F+CT S ++  GFT +M W A   ++  P F++G ++ +  V L 
Sbjct: 423 KYRVTTAKLSVGMETFSCTASVLIDAGFTKVMTWSAFGKDEPQPPFVQGTQVAINDVRLI 482

Query: 485 QGNTSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITL 544
           +  T PPDYL+ESELI+LME+HGIGTDASIPVHINNI +RNYV ++ GR+L+PT LGI L
Sbjct: 483 ESQTGPPDYLTESELITLMEEHGIGTDASIPVHINNICQRNYVHIENGRKLMPTTLGIVL 542

Query: 545 IRGYQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMD 604
           + GYQ IDP+L LP +R+ +E+ +T +A+G A+   V+++ ++ FK K+ YFVK ID MD
Sbjct: 543 VHGYQKIDPELVLPTMRTEVERMLTLIAQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMD 602

Query: 605 ALFEAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKEL 664
           ALFE  FSPLAESG+A S+CGKC RYMK+I ++P RL+C  C+E Y LP  G +K+Y+E 
Sbjct: 603 ALFEVSFSPLAESGKAHSRCGKCRRYMKYIQTKPARLHCSHCDETYALP-IGNVKVYREF 662

Query: 665 TCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMP 724
            CPLD+F+LL FS  G +G+S+P CPYCYN PPF  +  L G                  
Sbjct: 663 KCPLDDFDLLAFS-TGVKGRSYPFCPYCYNHPPFSDMPHLGG------------------ 722

Query: 725 CFLCPHPTCRHSLITQGVCACPEC-SGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIA 784
           C  C +  C HSL T G+ +C EC +G LVLD   AP WKL CN+C+ ++   +GA +I 
Sbjct: 723 CNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKLGCNRCDVIINCFKGATKIT 782

Query: 785 TTNEKCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKH----GKSFFR 844
               KC EC +  + V +    +  + G+    GCI C      L+E KH     +    
Sbjct: 783 VEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCSADFSHLVE-KHRAVASRPVRS 842

Query: 845 GRGSRGRGRGRGRGRGRGRGRSSG 857
           G G RG   GRG G   G    SG
Sbjct: 843 GGGFRGGKAGRGGGGMGGAAFGSG 842

BLAST of Cp4.1LG03g12320 vs. NCBI nr
Match: XP_023528530.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1783 bits (4617), Expect = 0.0
Identity = 868/868 (100.00%), Postives = 868/868 (100.00%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRGRGRGRGRSSGKLMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868

BLAST of Cp4.1LG03g12320 vs. NCBI nr
Match: KAG6582102.1 (DNA topoisomerase 3-beta, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018517.1 DNA topoisomerase 3-beta, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1771 bits (4586), Expect = 0.0
Identity = 863/868 (99.42%), Postives = 865/868 (99.65%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPARFQDWKATDPLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN+YRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           +RTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRGRGRGR  SSGKLMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGR--SSGKLMDPKMSFRDF 866

BLAST of Cp4.1LG03g12320 vs. NCBI nr
Match: XP_022955609.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita moschata])

HSP 1 Score: 1769 bits (4582), Expect = 0.0
Identity = 862/868 (99.31%), Postives = 865/868 (99.65%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPARFQDWKATDPLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN+YRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VR+LLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRVLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           +RTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRGRGRGR  SSGKLMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGR--SSGKLMDPKMSFRDF 866

BLAST of Cp4.1LG03g12320 vs. NCBI nr
Match: XP_022979706.1 (DNA topoisomerase 3-beta isoform X1 [Cucurbita maxima])

HSP 1 Score: 1765 bits (4571), Expect = 0.0
Identity = 861/868 (99.19%), Postives = 863/868 (99.42%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPARFQDWKATDPLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN+ RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNENRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSSV+VKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVLVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGID LYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDALYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRGRGRGR  SSGKLMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGR--SSGKLMDPKMSFRDF 866

BLAST of Cp4.1LG03g12320 vs. NCBI nr
Match: XP_038891105.1 (DNA topoisomerase 3-beta isoform X1 [Benincasa hispida])

HSP 1 Score: 1699 bits (4399), Expect = 0.0
Identity = 820/868 (94.47%), Postives = 848/868 (97.70%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGST+VHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTDVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPA FQDWK+TDPLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPAGFQDWKSTDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN+ RKKVYRARFSSVTEKDILKALDNLV+PN+NEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEIRKKVYRARFSSVTEKDILKALDNLVQPNKNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTF PEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFTPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRV+EDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQ+AMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+L+KN EWGDY
Sbjct: 301 LKVASSALGFGPQIAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLMKNSEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           IRTKIQFSIAGE+FNCTGS V+VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGELFNCTGSRVIVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL PTNLGITL+RG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLAPTNLGITLVRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQIT VAKGQADH SVVQNVLQQFKQKY YFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITLVAKGQADHSSVVQNVLQQFKQKYCYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQ+GAIKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           C HPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+T NE
Sbjct: 721 CLHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTRNE 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKS FRGRGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSLFRGRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRG GRGRGR+SG+ MDPKMSFRDF
Sbjct: 841 RGRGRG-GRGRGRNSGRPMDPKMSFRDF 867

BLAST of Cp4.1LG03g12320 vs. ExPASy TrEMBL
Match: A0A6J1GWS0 (DNA topoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111457562 PE=3 SV=1)

HSP 1 Score: 1769 bits (4582), Expect = 0.0
Identity = 862/868 (99.31%), Postives = 865/868 (99.65%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPARFQDWKATDPLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN+YRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNEYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VR+LLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRVLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           +RTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN
Sbjct: 421 LRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRGRGRGR  SSGKLMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGR--SSGKLMDPKMSFRDF 866

BLAST of Cp4.1LG03g12320 vs. ExPASy TrEMBL
Match: A0A6J1IRJ0 (DNA topoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111479340 PE=3 SV=1)

HSP 1 Score: 1765 bits (4571), Expect = 0.0
Identity = 861/868 (99.19%), Postives = 863/868 (99.42%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV
Sbjct: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDFPARFQDWKATDPLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFPARFQDWKATDPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN+ RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNENRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY
Sbjct: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           IRTKIQFSIAGEIFNCTGSSV+VKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSSVLVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP
Sbjct: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGID LYGAAKTNPSSGASSKGMGMPCFL
Sbjct: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDALYGAAKTNPSSGASSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG
Sbjct: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRGRGRGR  SSGKLMDPKMSFRDF
Sbjct: 841 RGRGRGRGRGR--SSGKLMDPKMSFRDF 866

BLAST of Cp4.1LG03g12320 vs. ExPASy TrEMBL
Match: A0A1S3AYH7 (DNA topoisomerase OS=Cucumis melo OX=3656 GN=LOC103483959 PE=3 SV=1)

HSP 1 Score: 1683 bits (4358), Expect = 0.0
Identity = 816/869 (93.90%), Postives = 841/869 (96.78%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDF A FQDWK T+PLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRV+EDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM  ATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           IRTKIQFSIAGEIFNCTGS V VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QIT VAKGQADH SVV++VLQQFKQKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQ+G IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGIDTLYGA+KTNPSSG SSKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGRG-RGRSSGKLMDPKMSFRDF 868
           RGRGRGRGRG RGR+SGK MDPKMSFRDF
Sbjct: 841 RGRGRGRGRGGRGRNSGKPMDPKMSFRDF 869

BLAST of Cp4.1LG03g12320 vs. ExPASy TrEMBL
Match: A0A5A7VKD3 (DNA topoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold70G00170 PE=3 SV=1)

HSP 1 Score: 1682 bits (4356), Expect = 0.0
Identity = 816/871 (93.69%), Postives = 841/871 (96.56%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVAS+VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHA+YKVTSVIGH+
Sbjct: 1   MVASTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHI 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDF A FQDWK T+PLDLFQASVIK ESNPKAHIRQHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKTTNPLDLFQASVIKTESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YIMQNGYELNLDWERG+LFDCDV  MFQKRV+EDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YIMQNGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM  ATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCSATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           IRTKIQFSIAGEIFNCTGS V VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QIT VAKGQADH SVV++VLQQFKQKYSYFVKKID MDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVESVLQQFKQKYSYFVKKIDRMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQ+G IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLI SLPGPEGKSFPLCPYCYNSPPFEGIDTLYGA+KTNPSSG SSKGMGMPCFL
Sbjct: 661 LDNFELLILSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGASKTNPSSGTSSKGMGMPCFL 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLPQGAHRI+TT++
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPQGAHRISTTSD 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHG SF R R SRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGNSFIRNRFSRGRG 840

Query: 841 RGRGRGRGRGRG---RSSGKLMDPKMSFRDF 868
           RGRGRGRGRGRG   R+SGK MDPKMSFRDF
Sbjct: 841 RGRGRGRGRGRGGRGRNSGKPMDPKMSFRDF 871

BLAST of Cp4.1LG03g12320 vs. ExPASy TrEMBL
Match: A0A0A0L9C7 (DNA topoisomerase OS=Cucumis sativus OX=3659 GN=Csa_3G172970 PE=3 SV=1)

HSP 1 Score: 1682 bits (4356), Expect = 0.0
Identity = 814/868 (93.78%), Postives = 841/868 (96.89%), Query Frame = 0

Query: 1   MVASSVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHV 60
           MVA +VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHA+YKVTSVIGHV
Sbjct: 1   MVAPTVLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAYYKVTSVIGHV 60

Query: 61  FSIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREG 120
           FSIDF A FQDWK+TDPLDLFQASVIK ESNPKAHI QHLSREARGCDCLVLWLDCDREG
Sbjct: 61  FSIDFQAGFQDWKSTDPLDLFQASVIKTESNPKAHICQHLSREARGCDCLVLWLDCDREG 120

Query: 121 ENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180
           ENICFEVIECTGFGGN  RKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID
Sbjct: 121 ENICFEVIECTGFGGNGTRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 180

Query: 181 LKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240
           LKVGV+FTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP
Sbjct: 181 LKVGVAFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCP 240

Query: 241 YIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNL 300
           YI+Q GYELNLDWERG+LFDCDV  MFQKRV+EDGIAEVT+ISEKQE+KVRPSGLNTVNL
Sbjct: 241 YILQTGYELNLDWERGKLFDCDVTAMFQKRVIEDGIAEVTEISEKQENKVRPSGLNTVNL 300

Query: 301 LKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDY 360
           LKVASSALG+GPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLH+LVKNPEWGDY
Sbjct: 301 LKVASSALGFGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHTLVKNPEWGDY 360

Query: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKY 420
           VRMLLSDGYSKPRSGTDVGDHPPITPM LATEDM+GRDAWRLYQYVCQHFIGSLSSDCKY
Sbjct: 361 VRMLLSDGYSKPRSGTDVGDHPPITPMCLATEDMLGRDAWRLYQYVCQHFIGSLSSDCKY 420

Query: 421 IRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGN 480
           IRTKIQFSIAGEIFNCTGS V VKGFTSIMPWLAVNEKKLPQFLKG+KIEVA+VDLYQGN
Sbjct: 421 IRTKIQFSIAGEIFNCTGSRVTVKGFTSIMPWLAVNEKKLPQFLKGEKIEVARVDLYQGN 480

Query: 481 TSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540
           T+PPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG
Sbjct: 481 TTPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRG 540

Query: 541 YQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALF 600
           YQCIDPDLCLPDIRSFIE QIT VAKGQADH SVVQ+VLQQF+QKYSYFVKKIDHMDALF
Sbjct: 541 YQCIDPDLCLPDIRSFIEHQITLVAKGQADHSSVVQSVLQQFRQKYSYFVKKIDHMDALF 600

Query: 601 EAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCP 660
           EAQFSPLA+SGRALSKCGKCLRYMKFISSQPQRLYCG CEEVYYLPQ+G IKLYKELTCP
Sbjct: 601 EAQFSPLADSGRALSKCGKCLRYMKFISSQPQRLYCGICEEVYYLPQKGTIKLYKELTCP 660

Query: 661 LDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFL 720
           LDNFELLI+SLPGPEGKSFPLCPYCYNSPPFEGI+TLYGA+K NPSSG SSKGMGMPCF+
Sbjct: 661 LDNFELLIYSLPGPEGKSFPLCPYCYNSPPFEGINTLYGASKANPSSGTSSKGMGMPCFM 720

Query: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNE 780
           CPHPTCRHSLITQGVCACPECSGTLVLDPV APNWKLNCNKCNCLVFLP+GAHRI+TT +
Sbjct: 721 CPHPTCRHSLITQGVCACPECSGTLVLDPVSAPNWKLNCNKCNCLVFLPKGAHRISTTRD 780

Query: 781 KCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRG 840
           KCA+CDSTIIEVDFNKKTTPLE GATLFVGCILCDEFLHSLLEMKHGKSF R RGSRGRG
Sbjct: 781 KCADCDSTIIEVDFNKKTTPLEDGATLFVGCILCDEFLHSLLEMKHGKSFIRNRGSRGRG 840

Query: 841 RGRGRGRGRGRGRSSGKLMDPKMSFRDF 868
           RGRGRGRG GRGRSSGK MDPKMSFRDF
Sbjct: 841 RGRGRGRG-GRGRSSGKPMDPKMSFRDF 867

BLAST of Cp4.1LG03g12320 vs. TAIR 10
Match: AT2G32000.1 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 660/863 (76.48%), Postives = 749/863 (86.79%), Query Frame = 0

Query: 6   VLMVAEKPSIALSIASALSHGQMSTRKGSTEVHEFDGRFLGHHAHYKVTSVIGHVFSIDF 65
           VLMVAEKPSIALSIAS LSHGQMSTR+GSTEVHEFDG F G  AHY+VTSVIGHVFS+DF
Sbjct: 7   VLMVAEKPSIALSIASVLSHGQMSTRRGSTEVHEFDGMFRGFKAHYRVTSVIGHVFSVDF 66

Query: 66  PARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREGENICF 125
           P ++Q+W   DP DLF A +IK ESNPKAHI +HLS EARGC  +VLWLDCDREGENICF
Sbjct: 67  PEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLDCDREGENICF 126

Query: 126 EVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEIDLKVGV 185
           EVIE TGF   D ++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDARQEIDLKVGV
Sbjct: 127 EVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDARQEIDLKVGV 186

Query: 186 SFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKFWSVCPYIMQN 245
           +F+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKFW++ PYI ++
Sbjct: 187 AFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKFWALRPYIRKD 246

Query: 246 GYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGLNTVNLLKVAS 305
           GYEL L+WER RLFD + AT+FQK V+E   A+V  +SEKQE K RP+GLNTVNLLKVAS
Sbjct: 247 GYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGLNTVNLLKVAS 306

Query: 306 SALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNPEWGDYVRMLL 365
           SALG+GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP WG YV+ LL
Sbjct: 307 SALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNPVWGGYVQRLL 366

Query: 366 SDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLSSDCKYIRTKI 425
           SDG+  P+SGTD GDHPPITPM  ATE MVG DAWRLYQYVCQHF+G++S +CKYIRTK+
Sbjct: 367 SDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVSPNCKYIRTKV 426

Query: 426 QFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVDLYQGNTSPPD 485
           + SI GE F+CTG  V  KGFT+IMPW AV+EKKLP FLKG++IEV +V+LY+GNT+PPD
Sbjct: 427 ELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVELYEGNTAPPD 486

Query: 486 YLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGITLIRGYQCID 545
           YL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGITLIRGYQCID
Sbjct: 487 YLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGITLIRGYQCID 546

Query: 546 PDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDHMDALFEAQFS 605
           PDLCLPDIRSFIEQQIT VAKGQADH  VVQ+V+QQF++K+SYFV++I+HMDALFEAQFS
Sbjct: 547 PDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEHMDALFEAQFS 606

Query: 606 PLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYKELTCPLDNFE 665
           PLA+SGRALSKCGKCLRYMK I++ P RL+CGTCEEVYYLPQ+G +KLYKELTCPLDNFE
Sbjct: 607 PLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYKELTCPLDNFE 666

Query: 666 LLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMGMPCFLCPHPT 725
           L+I+S+PGPEGKSFPLCPYCYNSPPFEGIDTL+GA+KT  +   +  G GMPC LCPHPT
Sbjct: 667 LVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAGMPCSLCPHPT 726

Query: 726 CRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRIATTNEKCAEC 785
           C+HS+  QGVCACPEC GTLVLDPV  P WKLNCN C+C+V LP+GAHRI TT+ +C EC
Sbjct: 727 CQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRITTTSNRCPEC 786

Query: 786 DSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRGSRGRGRGRGR 845
           DS IIE+DFNKKTTPLE GATL  GC+LCDE L SL+E+KHG+SF R RG RGRGRGRGR
Sbjct: 787 DSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RGGRGRGRGRGR 846

Query: 846 GRGRGRGRSSGKLMDPKMSFRDF 869
           GRG   GR   K +DPKMSFRDF
Sbjct: 847 GRG---GRRGSKSVDPKMSFRDF 865

BLAST of Cp4.1LG03g12320 vs. TAIR 10
Match: AT2G32000.2 (DNA topoisomerase, type IA, core )

HSP 1 Score: 1305.0 bits (3376), Expect = 0.0e+00
Identity = 612/813 (75.28%), Postives = 700/813 (86.10%), Query Frame = 0

Query: 59  HVFSI---DFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLD 118
           H+ S+   DFP ++Q+W   DP DLF A +IK ESNPKAHI +HLS EARGC  +VLWLD
Sbjct: 30  HLLSVMFSDFPEKYQNWATIDPQDLFDAPIIKKESNPKAHICRHLSNEARGCSYMVLWLD 89

Query: 119 CDREGENICFEVIECTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDA 178
           CDREGENICFEVIE TGF   D ++KVYRARFSSVTEKDI KA+DNLVEPNR+EALAVDA
Sbjct: 90  CDREGENICFEVIESTGFDMKDSKRKVYRARFSSVTEKDISKAMDNLVEPNRDEALAVDA 149

Query: 179 RQEIDLKVGVSFTRFQTTFFQGKYGNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 238
           RQEIDLKVGV+F+RFQT++FQGKY NLD R+ISYGPCQTPTLGFCVQR++ I TFKPEKF
Sbjct: 150 RQEIDLKVGVAFSRFQTSYFQGKYQNLDCRVISYGPCQTPTLGFCVQRYMHINTFKPEKF 209

Query: 239 WSVCPYIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQESKVRPSGL 298
           W++ PYI ++GYEL L+WER RLFD + AT+FQK V+E   A+V  +SEKQE K RP+GL
Sbjct: 210 WALRPYIRKDGYELQLEWERRRLFDLEAATVFQKLVVEGRTAKVMDVSEKQEVKGRPAGL 269

Query: 299 NTVNLLKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVKNP 358
           NTVNLLKVASSALG+GPQ AM LAERLYTQGFISYPRTESTAYPSSFDF  TL + V NP
Sbjct: 270 NTVNLLKVASSALGFGPQTAMHLAERLYTQGFISYPRTESTAYPSSFDFTDTLRAQVSNP 329

Query: 359 EWGDYVRMLLSDGYSKPRSGTDVGDHPPITPMSLATEDMVGRDAWRLYQYVCQHFIGSLS 418
            WG YV+ LLSDG+  P+SGTD GDHPPITPM  ATE MVG DAWRLYQYVCQHF+G++S
Sbjct: 330 VWGGYVQRLLSDGFHMPKSGTDAGDHPPITPMRAATEVMVGGDAWRLYQYVCQHFLGTVS 389

Query: 419 SDCKYIRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQKIEVAKVD 478
            +CKYIRTK++ SI GE F+CTG  V  KGFT+IMPW AV+EKKLP FLKG++IEV +V+
Sbjct: 390 PNCKYIRTKVELSIGGETFHCTGQRVTEKGFTAIMPWSAVDEKKLPSFLKGERIEVLRVE 449

Query: 479 LYQGNTSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRLVPTNLGI 538
           LY+GNT+PPDYL+ESELISLMEKHGIGTDASI VHINNIGERNYVQVQ+GR++VPT LGI
Sbjct: 450 LYEGNTAPPDYLTESELISLMEKHGIGTDASIAVHINNIGERNYVQVQSGRKMVPTALGI 509

Query: 539 TLIRGYQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKYSYFVKKIDH 598
           TLIRGYQCIDPDLCLPDIRSFIEQQIT VAKGQADH  VVQ+V+QQF++K+SYFV++I+H
Sbjct: 510 TLIRGYQCIDPDLCLPDIRSFIEQQITLVAKGQADHSHVVQHVIQQFRRKFSYFVQQIEH 569

Query: 599 MDALFEAQFSPLAESGRALSKCGKCLRYMKFISSQPQRLYCGTCEEVYYLPQRGAIKLYK 658
           MDALFEAQFSPLA+SGRALSKCGKCLRYMK I++ P RL+CGTCEEVYYLPQ+G +KLYK
Sbjct: 570 MDALFEAQFSPLADSGRALSKCGKCLRYMKHITAVPPRLFCGTCEEVYYLPQKGTVKLYK 629

Query: 659 ELTCPLDNFELLIFSLPGPEGKSFPLCPYCYNSPPFEGIDTLYGAAKTNPSSGASSKGMG 718
           ELTCPLDNFEL+I+S+PGPEGKSFPLCPYCYNSPPFEGIDTL+GA+KT  +   +  G G
Sbjct: 630 ELTCPLDNFELVIYSVPGPEGKSFPLCPYCYNSPPFEGIDTLFGASKTPNAPAKTKTGAG 689

Query: 719 MPCFLCPHPTCRHSLITQGVCACPECSGTLVLDPVGAPNWKLNCNKCNCLVFLPQGAHRI 778
           MPC LCPHPTC+HS+  QGVCACPEC GTLVLDPV  P WKLNCN C+C+V LP+GAHRI
Sbjct: 690 MPCSLCPHPTCQHSVRNQGVCACPECEGTLVLDPVSFPKWKLNCNLCSCIVLLPEGAHRI 749

Query: 779 ATTNEKCAECDSTIIEVDFNKKTTPLEGGATLFVGCILCDEFLHSLLEMKHGKSFFRGRG 838
            TT+ +C ECDS IIE+DFNKKTTPLE GATL  GC+LCDE L SL+E+KHG+SF R RG
Sbjct: 750 TTTSNRCPECDSAIIEIDFNKKTTPLENGATLHQGCVLCDELLLSLVEVKHGRSFVR-RG 809

Query: 839 SRGRGRGRGRGRGRGRGRSSGKLMDPKMSFRDF 869
            RGRGRGRGRGRG   GR   K +DPKMSFRDF
Sbjct: 810 GRGRGRGRGRGRG---GRRGSKSVDPKMSFRDF 838

BLAST of Cp4.1LG03g12320 vs. TAIR 10
Match: AT5G63920.1 (topoisomerase 3alpha )

HSP 1 Score: 406.0 bits (1042), Expect = 7.7e-113
Identity = 237/640 (37.03%), Postives = 351/640 (54.84%), Query Frame = 0

Query: 5   SVLMVAEKPSIALSIASALSHGQMSTRKGST---EVHEFDGRFLGHHAHYKVTSVIGHVF 64
           +VL VAEKPS+A S+A  LS G   TR+G +   ++ EFD    G      +TSVIGH+ 
Sbjct: 10  TVLNVAEKPSVAKSVAGILSRGTFRTREGRSRYNKIFEFDYAINGQPCRMLMTSVIGHLM 69

Query: 65  SIDFPARFQDWKATDPLDLFQASVIKAESNPKAHIRQHLSREARGCDCLVLWLDCDREGE 124
            ++F  R++ W + DP DL+QA V+K     K  I++ L  EAR  D LVLWLDCDREGE
Sbjct: 70  ELEFADRYRKWHSCDPADLYQAPVMKHVPEDKKDIKKTLEEEARKSDWLVLWLDCDREGE 129

Query: 125 NICFEVIE-CTGFGGNDYRKKVYRARFSSVTEKDILKALDNLVEPNRNEALAVDARQEID 184
           NI FEV++ C     N +   + RA FS++ ++DI +A+ NL +PN+  A AVDARQEID
Sbjct: 130 NIAFEVVDVCRAVKHNLF---IRRAHFSALIDRDIHEAVQNLRDPNQLFAEAVDARQEID 189

Query: 185 LKVGVSFTRFQTTFFQGKY-----GNLDSRLISYGPCQTPTLGFCVQRHLQITTFKPEKF 244
           L++G SFTRFQT   + ++     G   SR+ISYGPCQ PTLGF V+R+ +I   +PE+F
Sbjct: 190 LRIGASFTRFQTMLLRDRFAIDSTGEERSRVISYGPCQFPTLGFIVERYWEIQAHEPEEF 249

Query: 245 WSV-CPYIMQNGYELNLDWERGRLFDCDVATMFQKRVMEDGIAEVTQISEKQES-KVRPS 304
           W++ C +  + G     +W RG LFD   A +  +  +E+  A V  +   +E  K  P 
Sbjct: 250 WTINCSHQSEEGL-ATFNWMRGHLFDYASAVILYEMCVEEPTATVMNVPHPRERFKYPPY 309

Query: 305 GLNTVNLLKVASSALGYGPQMAMQLAERLYTQGFISYPRTESTAYPSSFDFKGTLHSLVK 364
            LNT+ L K AS       +  M++AE LY  GFISYPRTE+ ++ S  D +  +    +
Sbjct: 310 PLNTIELEKRASRYFRLSSEHTMKVAEELYQAGFISYPRTETDSFSSRTDLRAMVEEQTR 369

Query: 365 NPEWGDYVRMLLSD----GYSKPRSGTDVGDHPPITPMSLAT-EDMVGRDAWRLYQYVCQ 424
           +P WG Y + LL        +    G D   HPPI P   ++ E    RD   +Y+ V +
Sbjct: 370 HPAWGSYAQRLLEPEGGLWRNPANGGHDDKAHPPIHPTKFSSGESNWSRDHLNVYELVVR 429

Query: 425 HFIGSLSSDCKYIRTKIQFSIAGEIFNCTGSSVMVKGFTSIMPWLAVNEKKLPQFLKGQK 484
           H++  +S       T ++  IAGE F+ +G +++ K +  +  + +     +P + KGQ+
Sbjct: 430 HYLACVSQPAVAAETTVEIDIAGERFSASGRAILAKNYLEVYRFESWGGSVIPVYEKGQQ 489

Query: 485 IEVAKVDLYQGNTSPPDYLSESELISLMEKHGIGTDASIPVHINNIGERNYVQVQAGRRL 544
                + L    T PP  L E++L+S M+K GIGTDA++  HI  + +R Y    A  R 
Sbjct: 490 FIPTTLTLDAAVTRPPPLLCEADLLSCMDKAGIGTDATMHDHIKKLLDRGYATKDANTRF 549

Query: 545 VPTNLGITLIRGYQCIDPDLCLPDIRSFIEQQITFVAKGQADHFSVVQNVLQQFKQKY-S 604
            PTNLG  L+ GY  +  +L  P++R+ +E  +  V+ G+     V++  LQQ K  +  
Sbjct: 550 SPTNLGEALVMGYDDMGYELWKPNLRALMEHDMNEVSVGRKTKAEVLETCLQQMKACFLD 609

Query: 605 YFVKK---IDHMDALFE----AQFSPLAESGRALSKCGKC 621
             VKK   ++ M   FE       S    +G  + +C  C
Sbjct: 610 ARVKKSKLLEAMTIFFERSNNTDESESQTAGEVVRRCNLC 645