Cp4.1LG03g11700 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g11700
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionUbiquitin-conjugating enzyme E2 32
LocationCp4.1LG03: 10818695 .. 10821936 (-)
RNA-Seq ExpressionCp4.1LG03g11700
SyntenyCp4.1LG03g11700
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTGTCACGTTAAAACCCAATTTGCGATTCTCGTTCACATTTGCGGTTTGGCTTTGATTTCGATTCAAAGCCTACCGTCTGGTTCTGGGATCGCCTCTTGTTCTTTCCTTTTTAGAATCGTGATAGGATGGCGGTTGCAATCGGATCGGATCGGATCTGCGAGTGAATTGAAAACCTATGGCGGAGGATAAGTACAATCTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGTTGCAGTCGAATCCTTCCGATGATTTCACGAGTCTTCCTCTTGAGGTTTTTTGCTTTTGAATTGCTCTGGTTTTTGTTCCTGTTTTGTCGGTTTTACATTCTAATGGTTTGCGCATCTTTGTTGAATTAATTGATTCCCTGTGATTTTCGCCGATTGTTGCATTTGTATGATATATGTGGAAATGTGAGAAAGAAATCAATTATTAATCTGGTTTTCTTTTGATTTTTCATACCTGCTGGTGTCTGCTTCTATTGCGTATGAATGTTTTGATAGGGCTCAAAAAGGAAGAAAAGTGGAAATATTAACGGTTTGTTGCCAGATTTTTTATATGAAATTCATTCGATTTGATAATCATTGAATGTTCTTGAACAGCCGCAGTCCTTTGGTTTGAACCTTACTCTCTAGTTAGGCTAATAATCAATTTGTTTGAATTGATTACTGATTCTACAAATACACACTTGCATCCAGTCCTTTTCCATAAATCTTCCATGTATCTTATGCTATTCTCGCTATGTTCATGTGTGTTAATTCTTATAATTGTATCGACTTCATTGAATTTTGGTAAAAGAAGAACAATACCTGCTATGCGAACCATTGCTCGATTGATACACCAGTTCTGGTATTAATCCGCGTAGTTTTGTCTTTTTGACTGTCACAGAGTTTAAGGGTTTTGCCAAGGATAAATGTTGCTTTTTCAGGAGAATATATTTGAATGGCAATTTGCCATCCGTGGACCGGATGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATACAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGCTAACCGTAAGATAGCGATCTCTTTTCATGCTTGTTCCCCTTTCTGTTCCTTCTTGTCTTTTAAATGGAGCATGTTTGAGCTTGATTTTGAAAGACTTAAAATCGTTTTTGTCTTCTTTAGTACATCTTTAAACAAGATGGGTAACAGTCCAAGCCTACCGCTAGTAGATATTGTTTGCTTTGGCCCTTTACGTATCATTGTCAACCTCACGGTTTTAAAACACGTCTAATGGGGAGAGGTTTCCAGACCCTTATAAGGAATGATTTGTTCCTCTCTCTAACCAATGTAGGATCTCACAATCCCCCTTGGGGTCCCAGCGTCCTCGTTGTTATACCGCCTAATGTCTGACTCTGATACTAGTAACAGCCCAGGCTTACCGCTAGTAAATATTGTATGTTTTGGCTCATTAAGTATCGTCATCAGCCTTACGGTTTAAAACGCGTTCACTGGAAAAAGGTTTCCACACCTTTATAAGGCTTCTTTCCCCTCTTCAACCACTCTTGGATCTCACAAGATGCTCGTACAATCAAATTGATTTTGAATGATTAAAAGTTTTAGAGTTCTATCGCTGTCAGCCTCACGATTTAAAACGCGTCTACTAGGAAAAGGTTTCCACACCTTTATAAGGCTTCGTTCCCCTCTCCAACCACTCTTGGATCTCACAAGATGCTCGTACAATCAAATTGATTTTGTATGATTAAAAGTTTTAGAGTTGTATCGCCGTCAGACTCAGATATTTCCGTAAGCCTCATAGTTTAAAACACGTCCACTAGGAAAAGGTTTCCACACCTGTATAAGGCTTCATTCCCCTCTCCAATCACTCTTGGATCTCACAAGATGCTCGTATAATCAAATTGATTTTGAATGATTAAAAGTTTTAGAGTTATTTTGTGTGTTTATTTTGACACTTCCTTTCTTTCAGCCAAATGGGCGCTTTGAAACCCAAACGAAGATTTGCTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCGTCGTGGAGTGGTAGTTTTTTGTATCATTAATTCAACTTGTCTTGTTTTGTTATGTTAACTCATAGGATTTCCGTTGTCCGGTTTCTACATTTACGTTTAGTTGCTTCAAGTTCCACATGCTCTGATCTTTGTTCCTTTTTTCCCGCTTACCCTTTTCTGTTGATGCTTGTTCATAGTGCGCACTGCCTTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGAAAGAGCGACGAGAATTGGCCATCAAATCTCGAGAAGCGCCCCCCAGATTCGGTACTCCCGAACGTCAAAAGCTAATTAACGAGGTACTCCCGAGTTCTGTCGATATCTCGAAAGCTATGATACTGTTAGGTAATCAGCTTCTTTACGTCACAGATTCATGAATATCTGCTGAGTAAGGCGCCGCCTGTCCCTCGGCCGGGTTCTTCAGATGCCTCTGAGGAGAAACCCGCCAATGTCCAGGTAACTTCTGGAGCTGCTGTGGTAGAGATTCCGAATCCACCCGCAATCGACATGATTGTTGAGGAAGCAGTACCAGAGGGATTATCTGTGAATGTCAATCTCCAGCCTGAAGCAGCAAGAGTGGCAAGAGAGGTGCCATCGAGTCGCCCCACCGCTAAGGTGCAGCAGAGGCCGGAGACGAGAGTTCATAGGCCAGCGGATGACTGGTTGTTCAAATGGGCTGCCATCGGGCTCACTCTCGCAATTGTCGTTCTTTTGCTGAAGAAGTTCATGAAGGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAGATTACTGATATTTTTTGTCGGTTGATAGCTTTGCTCTGAGGCACAATGGTCGTATGTGTGTGAATATTTTAAGGGTAAATTTAAATGTCTTATAATTCAACAGGTAAAAAAGCAATCCAATGTTTAAATAGTATCCAAACACCTGAAGAACACAGCAACGAAGGCCAAGCCATCCATGAACATATGCATAATCACCATCTTAGAATAAGAGAATTCGAATGAGAAAATGTAGATTTAAGAGTGCTTTGTGGTGGGATGATGATGATGATGATCAGTGCTCATGATCTCCTTTCTTCACCACGGTGACTGGACATGCGCTATTGTTCACCACATAGTTGCTTACACTCCCCAATATTGCCCTAAA

mRNA sequence

CGTGTCACGTTAAAACCCAATTTGCGATTCTCGTTCACATTTGCGGTTTGGCTTTGATTTCGATTCAAAGCCTACCGTCTGGTTCTGGGATCGCCTCTTGTTCTTTCCTTTTTAGAATCGTGATAGGATGGCGGTTGCAATCGGATCGGATCGGATCTGCGAGTGAATTGAAAACCTATGGCGGAGGATAAGTACAATCTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGTTGCAGTCGAATCCTTCCGATGATTTCACGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGTGGACCGGATGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATACAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGCTAACCATTTGCTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCGTCGTGGAGTGTGCGCACTGCCTTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGAAAGAGCGACGAGAATTGGCCATCAAATCTCGAGAAGCGCCCCCCAGATTCGGTACTCCCGAACGTCAAAAGCTAATTAACGAGATTCATGAATATCTGCTGAGTAAGGCGCCGCCTGTCCCTCGGCCGGGTTCTTCAGATGCCTCTGAGGAGAAACCCGCCAATGTCCAGGTAACTTCTGGAGCTGCTGTGGTAGAGATTCCGAATCCACCCGCAATCGACATGATTGTTGAGGAAGCAGTACCAGAGGGATTATCTGTGAATGTCAATCTCCAGCCTGAAGCAGCAAGAGTGGCAAGAGAGGTGCCATCGAGTCGCCCCACCGCTAAGGTGCAGCAGAGGCCGGAGACGAGAGTTCATAGGCCAGCGGATGACTGGTTGTTCAAATGGGCTGCCATCGGGCTCACTCTCGCAATTGTCGTTCTTTTGCTGAAGAAGTTCATGAAGGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAGATTACTGATATTTTTTGTCGGTTGATAGCTTTGCTCTGAGGCACAATGGTCGTATGTGTGTGAATATTTTAAGGGTAAATTTAAATGTCTTATAATTCAACAGGTAAAAAAGCAATCCAATGTTTAAATAGTATCCAAACACCTGAAGAACACAGCAACGAAGGCCAAGCCATCCATGAACATATGCATAATCACCATCTTAGAATAAGAGAATTCGAATGAGAAAATGTAGATTTAAGAGTGCTTTGTGGTGGGATGATGATGATGATGATCAGTGCTCATGATCTCCTTTCTTCACCACGGTGACTGGACATGCGCTATTGTTCACCACATAGTTGCTTACACTCCCCAATATTGCCCTAAA

Coding sequence (CDS)

ATGGCGGAGGATAAGTACAATCTGAAGAACCCTGCGGTGAAGAGGATCCTTCAGGAGGTCAAGGAGTTGCAGTCGAATCCTTCCGATGATTTCACGAGTCTTCCTCTTGAGGAGAATATATTTGAATGGCAATTTGCCATCCGTGGACCGGATGATAGTGAATTTGAGGGTGGGATATATCATGGACGTATACAATTGCCAGCAGAATATCCATTCAAGCCACCTTCATTCATGTTGCTAACCATTTGCTTGAGCATATCAAATCACCATCCAGAGCATTGGCAACCGTCGTGGAGTGTGCGCACTGCCTTGGTTGCATTAATTGCTTTCATGCCCACAAACCCAAATGGCGCTCTTGGTTCCCTGGACTACAAGAAGAAAGAGCGACGAGAATTGGCCATCAAATCTCGAGAAGCGCCCCCCAGATTCGGTACTCCCGAACGTCAAAAGCTAATTAACGAGATTCATGAATATCTGCTGAGTAAGGCGCCGCCTGTCCCTCGGCCGGGTTCTTCAGATGCCTCTGAGGAGAAACCCGCCAATGTCCAGGTAACTTCTGGAGCTGCTGTGGTAGAGATTCCGAATCCACCCGCAATCGACATGATTGTTGAGGAAGCAGTACCAGAGGGATTATCTGTGAATGTCAATCTCCAGCCTGAAGCAGCAAGAGTGGCAAGAGAGGTGCCATCGAGTCGCCCCACCGCTAAGGTGCAGCAGAGGCCGGAGACGAGAGTTCATAGGCCAGCGGATGACTGGTTGTTCAAATGGGCTGCCATCGGGCTCACTCTCGCAATTGTCGTTCTTTTGCTGAAGAAGTTCATGAAGGCAAGCGCTCATGGTTCTGTGTTCATGGATGAATCTTAG

Protein sequence

MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPGSSDASEEKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPSSRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Homology
[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy Swiss-Prot
Match: Q9LSP7 (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 3.6e-96
Identity = 184/307 (59.93%), Postives = 223/307 (72.64%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKE+Q+NPSDDF SLPLEENIFEWQFAIRGP D+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLT          ICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPT+PNGALGS+DY K ERR LAIKSRE PP++G+PERQK+I+EIH+Y+LSKA  VP+P 
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SSDASE------EKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARV 240
             + S+      E  + V+      VVE  +    D IV++ + E  +  VN        
Sbjct: 181 PLECSQAPSIVSEAHSQVEPQEAITVVEERSIATTDTIVDDQIIEETAEAVNTAASVVPA 240

Query: 241 AREVPSSRPTAKVQQRPETRVHR-----PADDWLFKWAAIGLTLAIVVLLLKKFMKASAH 287
           A  +P+     K     E R+ R     P DD LF WAA+GLT+AI+VLLLKKF+K++ +
Sbjct: 241 AAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKSNGY 300

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy Swiss-Prot
Match: Q9Y385 (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-47
Identity = 88/146 (60.27%), Postives = 112/146 (76.71%), Query Frame = 0 

Query: 3   EDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  EL+ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT          ICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKKERRELAIKSRE 139
           T   GA+GSLDY  +ERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy Swiss-Prot
Match: Q9JJZ4 (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-47
Identity = 88/146 (60.27%), Postives = 112/146 (76.71%), Query Frame = 0 

Query: 3   EDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHG 62
           E +YNLK+PAVKR+++E  EL+ +P+D + + PLE+N+FEW F +RGP DS+F+GG+YHG
Sbjct: 2   ETRYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHG 61

Query: 63  RIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFMP 122
           RI LP EYP KPPS +LLT          ICLSIS HHPE WQPSWS+RTAL+A+I FMP
Sbjct: 62  RIVLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMP 121

Query: 123 TNPNGALGSLDYKKKERRELAIKSRE 139
           T   GA+GSLDY  +ERR LA KS++
Sbjct: 122 TKGEGAIGSLDYTPEERRALAKKSQD 146

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy Swiss-Prot
Match: Q6FQK7 (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 3.3e-25
Identity = 54/135 (40.00%), Postives = 81/135 (60.00%), Query Frame = 0 

Query: 12  AVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K +  NP     + P EENI EW + I GP D+ ++GG YHG +  P++YP
Sbjct: 6   AQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTFPSDYP 65

Query: 72  FKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           +KPP+  ++T          +CLS+S++HP+ W P WSV T L  L++FM T      GS
Sbjct: 66  YKPPAIRMITPNGRFKENTRLCLSMSDYHPDTWNPGWSVATILNGLLSFM-TGDESTTGS 125

Query: 132 LDYKKKERRELAIKS 137
           +   ++E++ LA KS
Sbjct: 126 ITTTQQEKKILAKKS 139

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy Swiss-Prot
Match: Q74Z34 (Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=UBC6 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.3e-24
Identity = 52/137 (37.96%), Postives = 81/137 (59.12%), Query Frame = 0 

Query: 12  AVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYP 71
           A KR+ +E K +  NP     + P E+NI  W + I GP ++ +E G YHG +  P +YP
Sbjct: 6   AYKRLSKEYKMMTENPPPYIVAAPKEDNILVWHYVITGPPETPYEDGQYHGTLVFPNDYP 65

Query: 72  FKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGS 131
           F PP+  +LT          +CLS+S++HP+ W PSWSV T L  L++FM T+   ++G+
Sbjct: 66  FNPPAIRMLTPNGRFRENTRLCLSMSDYHPDTWNPSWSVATILTGLLSFMTTD-ESSIGT 125

Query: 132 LDYKKKERRELAIKSRE 139
           ++     +R+ A KS+E
Sbjct: 126 MESTDSTKRKYAAKSKE 141

[Close]
BLAST of Cp4.1LG03g11700 vs. NCBI nr
Match: XP_023527846.1 (ubiquitin-conjugating enzyme E2 32-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566 bits (1458), Expect = 4.23e-203
Identity = 287/297 (96.63%), Postives = 287/297 (96.63%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG
Sbjct: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180

Query: 181 SSDASEEKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPS 240
           SSDASEEKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPS
Sbjct: 181 SSDASEEKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPS 240

Query: 241 SRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 287
           SRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 SRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 297

[Close]
BLAST of Cp4.1LG03g11700 vs. NCBI nr
Match: KAG7018574.1 (Ubiquitin-conjugating enzyme E2 32, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 549 bits (1415), Expect = 4.57e-196
Identity = 276/293 (94.20%), Postives = 282/293 (96.25%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYNL NPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 35  MAEDKYNLNNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 94

Query: 61  HGRIQLPAEYPFKPPSFMLLTICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALG 120
           HGRIQLPAEYPFKPPSFMLLTICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALG
Sbjct: 95  HGRIQLPAEYPFKPPSFMLLTICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALG 154

Query: 121 SLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPGSSDASEEKPA 180
           SLDYKKKERRELAIKSREAPP+FGTPERQKLINEIHEYLLSK PPVP+PGSS+ASEEKPA
Sbjct: 155 SLDYKKKERRELAIKSREAPPKFGTPERQKLINEIHEYLLSKVPPVPQPGSSNASEEKPA 214

Query: 181 NVQVTSGAA------VVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPSSRPT 240
           N QVTSGAA      V+EIPNPPAIDMIVEEA P+GLSVNVNLQPEAARVAREVPSSRPT
Sbjct: 215 NAQVTSGAAEVAAAAVLEIPNPPAIDMIVEEAAPKGLSVNVNLQPEAARVAREVPSSRPT 274

Query: 241 AKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 287
           A+VQQRPET VHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 275 AQVQQRPETGVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 327

[Close]
BLAST of Cp4.1LG03g11700 vs. NCBI nr
Match: XP_022955717.1 (ubiquitin-conjugating enzyme E2 32-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 542 bits (1396), Expect = 1.49e-193
Identity = 277/303 (91.42%), Postives = 282/303 (93.07%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYNL NPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNLNNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKKKERRELAIKSREAPP+FGTPERQKLINEIHEYLLSK PPVP+PG
Sbjct: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPKFGTPERQKLINEIHEYLLSKVPPVPQPG 180

Query: 181 SSDASEEKPANVQVTSGAA------VVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARV 240
           SS+ASEEKPANVQVTSGAA      VVEIPNPPAIDMIVEEA PEGLSVNVNLQPEAARV
Sbjct: 181 SSNASEEKPANVQVTSGAAEVAAAAVVEIPNPPAIDMIVEEAAPEGLSVNVNLQPEAARV 240

Query: 241 AREVPSSRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 287
           AREVPSSRPTA+VQQRPET  HRPA+DWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM
Sbjct: 241 AREVPSSRPTAQVQQRPETGAHRPANDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 300

[Close]
BLAST of Cp4.1LG03g11700 vs. NCBI nr
Match: XP_023527847.1 (ubiquitin-conjugating enzyme E2 32-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527 bits (1358), Expect = 4.41e-188
Identity = 273/297 (91.92%), Postives = 273/297 (91.92%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINE              PG
Sbjct: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINE--------------PG 180

Query: 181 SSDASEEKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPS 240
           SSDASEEKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPS
Sbjct: 181 SSDASEEKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARVAREVPS 240

Query: 241 SRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 287
           SRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 241 SRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFMDES 283

[Close]
BLAST of Cp4.1LG03g11700 vs. NCBI nr
Match: XP_022955718.1 (ubiquitin-conjugating enzyme E2 32-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 506 bits (1304), Expect = 9.31e-180
Identity = 265/303 (87.46%), Postives = 269/303 (88.78%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYNL NPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNLNNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKKKERRELAIKSREAPP+FGTPERQKLINE              PG
Sbjct: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPKFGTPERQKLINE--------------PG 180

Query: 181 SSDASEEKPANVQVTSGAA------VVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARV 240
           SS+ASEEKPANVQVTSGAA      VVEIPNPPAIDMIVEEA PEGLSVNVNLQPEAARV
Sbjct: 181 SSNASEEKPANVQVTSGAAEVAAAAVVEIPNPPAIDMIVEEAAPEGLSVNVNLQPEAARV 240

Query: 241 AREVPSSRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 287
           AREVPSSRPTA+VQQRPET  HRPA+DWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM
Sbjct: 241 AREVPSSRPTAQVQQRPETGAHRPANDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 289

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy TrEMBL
Match: A0A6J1GUE3 (ubiquitin-conjugating enzyme E2 32-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457632 PE=4 SV=1)

HSP 1 Score: 542 bits (1396), Expect = 7.20e-194
Identity = 277/303 (91.42%), Postives = 282/303 (93.07%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYNL NPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNLNNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKKKERRELAIKSREAPP+FGTPERQKLINEIHEYLLSK PPVP+PG
Sbjct: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPKFGTPERQKLINEIHEYLLSKVPPVPQPG 180

Query: 181 SSDASEEKPANVQVTSGAA------VVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARV 240
           SS+ASEEKPANVQVTSGAA      VVEIPNPPAIDMIVEEA PEGLSVNVNLQPEAARV
Sbjct: 181 SSNASEEKPANVQVTSGAAEVAAAAVVEIPNPPAIDMIVEEAAPEGLSVNVNLQPEAARV 240

Query: 241 AREVPSSRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 287
           AREVPSSRPTA+VQQRPET  HRPA+DWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM
Sbjct: 241 AREVPSSRPTAQVQQRPETGAHRPANDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 300

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy TrEMBL
Match: A0A6J1GUS1 (ubiquitin-conjugating enzyme E2 32-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457632 PE=4 SV=1)

HSP 1 Score: 506 bits (1304), Expect = 4.51e-180
Identity = 265/303 (87.46%), Postives = 269/303 (88.78%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYNL NPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNLNNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKKKERRELAIKSREAPP+FGTPERQKLINE              PG
Sbjct: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPKFGTPERQKLINE--------------PG 180

Query: 181 SSDASEEKPANVQVTSGAA------VVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARV 240
           SS+ASEEKPANVQVTSGAA      VVEIPNPPAIDMIVEEA PEGLSVNVNLQPEAARV
Sbjct: 181 SSNASEEKPANVQVTSGAAEVAAAAVVEIPNPPAIDMIVEEAAPEGLSVNVNLQPEAARV 240

Query: 241 AREVPSSRPTAKVQQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 287
           AREVPSSRPTA+VQQRPET  HRPA+DWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM
Sbjct: 241 AREVPSSRPTAQVQQRPETGAHRPANDWLFKWAAIGLTLAIVVLLLKKFMKASAHGSVFM 289

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy TrEMBL
Match: A0A6J1GX37 (ubiquitin-conjugating enzyme E2 32-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111457632 PE=4 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 1.03e-167
Identity = 242/270 (89.63%), Postives = 248/270 (91.85%), Query Frame = 0 

Query: 34  LPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLT----------IC 93
           L  +ENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLT          IC
Sbjct: 2   LLFQENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKIC 61

Query: 94  LSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKKERRELAIKSREAPPRF 153
           LSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKKERRELAIKSREAPP+F
Sbjct: 62  LSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSLDYKKKERRELAIKSREAPPKF 121

Query: 154 GTPERQKLINEIHEYLLSKAPPVPRPGSSDASEEKPANVQVTSGAA------VVEIPNPP 213
           GTPERQKLINEIHEYLLSK PPVP+PGSS+ASEEKPANVQVTSGAA      VVEIPNPP
Sbjct: 122 GTPERQKLINEIHEYLLSKVPPVPQPGSSNASEEKPANVQVTSGAAEVAAAAVVEIPNPP 181

Query: 214 AIDMIVEEAVPEGLSVNVNLQPEAARVAREVPSSRPTAKVQQRPETRVHRPADDWLFKWA 273
           AIDMIVEEA PEGLSVNVNLQPEAARVAREVPSSRPTA+VQQRPET  HRPA+DWLFKWA
Sbjct: 182 AIDMIVEEAAPEGLSVNVNLQPEAARVAREVPSSRPTAQVQQRPETGAHRPANDWLFKWA 241

Query: 274 AIGLTLAIVVLLLKKFMKASAHGSVFMDES 287
           AIGLTLAIVVLLLKKFMKASAHGSVFMDES
Sbjct: 242 AIGLTLAIVVLLLKKFMKASAHGSVFMDES 271

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy TrEMBL
Match: A0A5A7U7K5 (Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold209G001470 PE=4 SV=1)

HSP 1 Score: 463 bits (1192), Expect = 6.93e-163
Identity = 247/307 (80.46%), Postives = 261/307 (85.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKE+Q+NPSDDF SLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKK+ERR LAIKSREAPP+FGTPERQKLI+EIHEY+LSKAPPVP+ G
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDASEEKPAN----VQVTS-----GAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEA 240
           SSD S+EKP N    VQVTS      AA  E+ NPP I+ IVEEAVPE       +QPE 
Sbjct: 181 SSDVSDEKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPE-------VQPEV 240

Query: 241 ARVAREVPSSRPTAKV-QQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHG 287
           ARVA   PSSRPT +  QQRPETRVH+PADD LF WAAIGLTLAIVVLLLKKFMKASAHG
Sbjct: 241 ARVA---PSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHG 297

[Close]
BLAST of Cp4.1LG03g11700 vs. ExPASy TrEMBL
Match: A0A1S3AXJ7 (ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 SV=1)

HSP 1 Score: 462 bits (1189), Expect = 1.98e-162
Identity = 246/307 (80.13%), Postives = 261/307 (85.02%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MAEDKYN+KNPAVKRILQEVKE+Q+NPSDDF SLPLEENIFEWQFAIRGPDDSEFEGGIY
Sbjct: 1   MAEDKYNMKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPDDSEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPAEYPFKPPSFMLLT          ICLSISNHHPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPTNPNGALGSLDYKK+ERR LAIKSREAPP+FGTPERQKLI+EIHEY+LSKAPPVP+ G
Sbjct: 121 MPTNPNGALGSLDYKKEERRVLAIKSREAPPKFGTPERQKLIDEIHEYMLSKAPPVPQSG 180

Query: 181 SSDASEEKPAN----VQVTS-----GAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEA 240
           SSD S++KP N    VQVTS      AA  E+ NPP I+ IVEEAVPE       +QPE 
Sbjct: 181 SSDVSDQKPTNEESDVQVTSEPAELAAAGEELQNPPTINRIVEEAVPE-------VQPEV 240

Query: 241 ARVAREVPSSRPTAKV-QQRPETRVHRPADDWLFKWAAIGLTLAIVVLLLKKFMKASAHG 287
           ARVA   PSSRPT +  QQRPETRVH+PADD LF WAAIGLTLAIVVLLLKKFMKASAHG
Sbjct: 241 ARVA---PSSRPTTQAEQQRPETRVHKPADDRLFTWAAIGLTLAIVVLLLKKFMKASAHG 297

[Close]
BLAST of Cp4.1LG03g11700 vs. TAIR 10
Match: AT3G17000.1 (ubiquitin-conjugating enzyme 32 )

HSP 1 Score: 352.8 bits (904), Expect = 2.6e-97
Identity = 184/307 (59.93%), Postives = 223/307 (72.64%), Query Frame = 0 

Query: 1   MAEDKYNLKNPAVKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIY 60
           MA+++YN KNPAVKRILQEVKE+Q+NPSDDF SLPLEENIFEWQFAIRGP D+EFEGGIY
Sbjct: 1   MADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIY 60

Query: 61  HGRIQLPAEYPFKPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAF 120
           HGRIQLPA+YPFKPPSFMLLT          ICLSISN+HPEHWQPSWSVRTALVALIAF
Sbjct: 61  HGRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAF 120

Query: 121 MPTNPNGALGSLDYKKKERRELAIKSREAPPRFGTPERQKLINEIHEYLLSKAPPVPRPG 180
           MPT+PNGALGS+DY K ERR LAIKSRE PP++G+PERQK+I+EIH+Y+LSKA  VP+P 
Sbjct: 121 MPTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPL 180

Query: 181 SSDASE------EKPANVQVTSGAAVVEIPNPPAIDMIVEEAVPEGLSVNVNLQPEAARV 240
             + S+      E  + V+      VVE  +    D IV++ + E  +  VN        
Sbjct: 181 PLECSQAPSIVSEAHSQVEPQEAITVVEERSIATTDTIVDDQIIEETAEAVNTAASVVPA 240

Query: 241 AREVPSSRPTAKVQQRPETRVHR-----PADDWLFKWAAIGLTLAIVVLLLKKFMKASAH 287
           A  +P+     K     E R+ R     P DD LF WAA+GLT+AI+VLLLKKF+K++ +
Sbjct: 241 AAPLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKSNGY 300

[Close]
BLAST of Cp4.1LG03g11700 vs. TAIR 10
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 102.1 bits (253), Expect = 7.8e-22
Identity = 48/134 (35.82%), Postives = 73/134 (54.48%), Query Frame = 0 

Query: 13  VKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + L   P     + P   +I EW + + G + + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + T          ICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKKERRELAIKS 137
           +    E++ LA  S
Sbjct: 127 NTSVAEKQRLAKSS 139

[Close]
BLAST of Cp4.1LG03g11700 vs. TAIR 10
Match: AT5G50430.2 (ubiquitin-conjugating enzyme 33 )

HSP 1 Score: 102.1 bits (253), Expect = 7.8e-22
Identity = 48/134 (35.82%), Postives = 73/134 (54.48%), Query Frame = 0 

Query: 13  VKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + L   P     + P   +I EW + + G + + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFMPTNPNGALGSL 132
           KPP   + T          ICLS+S+ HPE W P WSV + L  L++FM  N +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDN-SPTTGSV 126

Query: 133 DYKKKERRELAIKS 137
           +    E++ LA  S
Sbjct: 127 NTSVAEKQRLAKSS 139

[Close]
BLAST of Cp4.1LG03g11700 vs. TAIR 10
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 99.8 bits (247), Expect = 3.9e-21
Identity = 56/191 (29.32%), Postives = 89/191 (46.60%), Query Frame = 0 

Query: 13  VKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + L   P     + P   +I EW + + G + + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFM----PTNPNGA 132
           KPP   + T          ICLS+S+ HPE W P WSV + L  L++FM    PT  +  
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSPTTGSVN 126

Query: 133 LGSLDYKKKERRELAIKSREAPPRFGTPERQKLIN--EIHEYLLSKAPPVPRPGSSDASE 188
              ++ ++  +  LA   +    R   PE  +  N  ++ E   ++      P  SD   
Sbjct: 127 TTVIEKQRLAKSSLAFNCKTPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKSDTKV 186

[Close]
BLAST of Cp4.1LG03g11700 vs. TAIR 10
Match: AT1G17280.2 (ubiquitin-conjugating enzyme 34 )

HSP 1 Score: 99.8 bits (247), Expect = 3.9e-21
Identity = 56/191 (29.32%), Postives = 89/191 (46.60%), Query Frame = 0 

Query: 13  VKRILQEVKELQSNPSDDFTSLPLEENIFEWQFAIRGPDDSEFEGGIYHGRIQLPAEYPF 72
           +KR+ +E + L   P     + P   +I EW + + G + + F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 73  KPPSFMLLT----------ICLSISNHHPEHWQPSWSVRTALVALIAFM----PTNPNGA 132
           KPP   + T          ICLS+S+ HPE W P WSV + L  L++FM    PT  +  
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSPTTGSVN 126

Query: 133 LGSLDYKKKERRELAIKSREAPPRFGTPERQKLIN--EIHEYLLSKAPPVPRPGSSDASE 188
              ++ ++  +  LA   +    R   PE  +  N  ++ E   ++      P  SD   
Sbjct: 127 TTVIEKQRLAKSSLAFNCKTPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKSDTKV 186

The following BLAST results are available for this feature:
BLAST of Cp4.1LG03g11700 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LSP73.6e-9659.93Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana OX=3702 GN=UBC32 PE=2... [more]
Q9Y3852.4e-4760.27Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens OX=9606 GN=UBE2J1 PE=1 SV=2[more]
Q9JJZ42.4e-4760.27Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus OX=10090 GN=Ube2j1 PE=1 SV=2[more]
Q6FQK73.3e-2540.00Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata (strain ATCC 2001 / CBS 13... [more]
Q74Z341.3e-2437.96Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 10... [more]
BLAST of Cp4.1LG03g11700 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023527846.14.23e-20396.63ubiquitin-conjugating enzyme E2 32-like isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7018574.14.57e-19694.20Ubiquitin-conjugating enzyme E2 32, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022955717.11.49e-19391.42ubiquitin-conjugating enzyme E2 32-like isoform X1 [Cucurbita moschata][more]
XP_023527847.14.41e-18891.92ubiquitin-conjugating enzyme E2 32-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022955718.19.31e-18087.46ubiquitin-conjugating enzyme E2 32-like isoform X2 [Cucurbita moschata][more]
BLAST of Cp4.1LG03g11700 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GUE37.20e-19491.42ubiquitin-conjugating enzyme E2 32-like isoform X1 OS=Cucurbita moschata OX=3662... [more]
A0A6J1GUS14.51e-18087.46ubiquitin-conjugating enzyme E2 32-like isoform X2 OS=Cucurbita moschata OX=3662... [more]
A0A6J1GX371.03e-16789.63ubiquitin-conjugating enzyme E2 32-like isoform X3 OS=Cucurbita moschata OX=3662... [more]
A0A5A7U7K56.93e-16380.46Ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3AXJ71.98e-16280.13ubiquitin-conjugating enzyme E2 32 OS=Cucumis melo OX=3656 GN=LOC103483888 PE=4 ... [more]
BLAST of Cp4.1LG03g11700 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G17000.12.6e-9759.93ubiquitin-conjugating enzyme 32 [more]
AT5G50430.17.8e-2235.82ubiquitin-conjugating enzyme 33 [more]
AT5G50430.27.8e-2235.82ubiquitin-conjugating enzyme 33 [more]
AT1G17280.13.9e-2129.32ubiquitin-conjugating enzyme 34 [more]
AT1G17280.23.9e-2129.32ubiquitin-conjugating enzyme 34 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 14..164
e-value: 1.8E-19
score: 80.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 224..243
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 162..183
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 7..287
NoneNo IPR availablePANTHERPTHR24068:SF303BNAC01G21910D PROTEINcoord: 7..287
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 2..162
e-value: 9.3E-40
score: 138.0
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 12..122
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 15..116
e-value: 1.0E-16
score: 60.8
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 11..156
score: 23.144825
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 13..138
e-value: 7.03315E-40
score: 133.867

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g11700.1Cp4.1LG03g11700.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0061631 ubiquitin conjugating enzyme activity