Cp4.1LG03g10420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g10420
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPectin lyase-like superfamily protein
LocationCp4.1LG03: 11638772 .. 11640602 (-)
RNA-Seq ExpressionCp4.1LG03g10420
SyntenyCp4.1LG03g10420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCAAGAATGAAGCTTCTTGTTTCCTTTGTTTTCCTCTTTTCCATTGCTGTTTTAAGCTCATTGACAGAATCAGCTTCGTCGTATTTCAATGTTCTTGATTATGGAGCAAATGGAAATGGCAAAAAAGATGATTCAGAAGTAAGTAGTTTAATCTGAATGTCGATTTTTTAAAGAAGGGTTCTCTCTTTATACTTGTAATGACTTGTTTTATTTGTCTTTCTCTATGTCAAGGCTTTTTTGAAAGCATGGAATGATGTATGTGCATCAACAGAAGACAACCCAACGTTACGTGTACCATCTGGAAAAACTTTCCTATTGAACCCTCTCAAATTCCAGGGTCCTTGCAAGTCCAAACGAATCAATTTTGATGTAGCTTACCTAACCCCGTTTCTCATAAAATTGAAATTGAAAAATTTATGGTAAATTTATACACTATTTTGTGATTTTATAGCTTGGTGGAAGTGTAATGGCTCCAACCAAGGATGAATGGTCCGGAGATGGTGTTGATAAATGGATCCAATTTTACGACATAGATTCTCTCACCGTTAAAGGAGATGGACGGTTTGATGGCCAAGGTTCTTCTTGGTGGGACAAAGATTGTCATAAACATTGCAAACGGCCAACGGTAACTTGTTATTATTATTATTATTTTCTTTTAACCTTTTTACTTTTTTTTAATTATTACTACGGGTTAAATTTATAATATATGATTTTGCAGGCAATATTTTTCCACAACTGCAACGGTCTTCATCTAAGTAAAACGAAGCATGTTAATAGTGGCAAAAATCATATGTCCATAAATTGTTGCAATGATGTAATTATTTCAGATATTCATATTACTGCTCCTGATGAGAGTCCTAATACGGATGGAATTGATATTTCTGGATCAAAAAATGTTCGTATTCAAGATTCTTTTATAGGCACAGGTAACAAATTAATGTTATGATTATCCGTAATTATTAAGTACATAATTTTATGTTTTTATTTATTGACTTCAATGTTAAATATTGCAGGTGATGATTGTGTTGCAATTAATGATGGATCTTCAAATATCGATATAACAGGTCTCACTTGTGGCCCCGGTCACGGCATAAGGTAAAAATTACATTTTTTTTTCCATGTTTGATATTCATTGAAATTTTTAATTTTTTTAATATATTTTGTGGTGTTTATTTGACTGAAGCATTGGGAGCTTAGGAAAAAACGGTGATTATAATGTTGTTGAAAATGTCTATGTAAGTGATTGTATCTTACAAGATACTCAAAACGGAGTTAGAATCAAGACTTGGGAGGTGATAATACGTTTTAATTTATTTATAAATAATATTTAATATACCATATAAAGTTTGTAAATATTTTTTAATCGTAATTACAGGGTGGATATGGATATGCAAAAAATATCACATTCGAGAAAATAACACTCGAAAATGTAAAAAACCCAATCATTATCGATCAACATTACACAGTTTATGCATATGGAAGGAAAAATAAGGTACACAACAGATCAATTTCATCCCGTTCTCATTATTATTATTATTATTTTTATTATTTTTTTGTCATAGTTTATTATATTTGGTTTTTATGTTGGTTTGTTTGTAGGGAAGGGAAATAAAAGTGAGCGATGTGACATATCGTAACGTTCATGGAACATCAGCAAATGAAAAGGCAATTACATTGGACTGTTCTCGAGCTCGTTGCACAAATATTATTATGGAAGAAGTGAACATTACGACATCAACTTCTGACGAAGAAGCCAAATCTTTTTGCCAAAATGCTGATGGAAAATCCACATCTACAGTTCCACCGGTGCCTTGTTTATCAAAATCTCAT

mRNA sequence

CTCAAGAATGAAGCTTCTTGTTTCCTTTGTTTTCCTCTTTTCCATTGCTGTTTTAAGCTCATTGACAGAATCAGCTTCGTCGTATTTCAATGTTCTTGATTATGGAGCAAATGGAAATGGCAAAAAAGATGATTCAGAAGCTTTTTTGAAAGCATGGAATGATGTATGTGCATCAACAGAAGACAACCCAACGTTACGTGTACCATCTGGAAAAACTTTCCTATTGAACCCTCTCAAATTCCAGGGTCCTTGCAAGTCCAAACGAATCAATTTTGATCTTGGTGGAAGTGTAATGGCTCCAACCAAGGATGAATGGTCCGGAGATGGTGTTGATAAATGGATCCAATTTTACGACATAGATTCTCTCACCGTTAAAGGAGATGGACGGTTTGATGGCCAAGGTTCTTCTTGGTGGGACAAAGATTGTCATAAACATTGCAAACGGCCAACGGCAATATTTTTCCACAACTGCAACGGTCTTCATCTAAGTAAAACGAAGCATGTTAATAGTGGCAAAAATCATATGTCCATAAATTGTTGCAATGATGTAATTATTTCAGATATTCATATTACTGCTCCTGATGAGAGTCCTAATACGGATGGAATTGATATTTCTGGATCAAAAAATGTTCGTATTCAAGATTCTTTTATAGGCACAGGTGATGATTGTGTTGCAATTAATGATGGATCTTCAAATATCGATATAACAGGTCTCACTTGTGGCCCCGGTCACGGCATAAGCATTGGGAGCTTAGGAAAAAACGGTGATTATAATGTTGTTGAAAATGTCTATGTAAGTGATTGTATCTTACAAGATACTCAAAACGGAGTTAGAATCAAGACTTGGGAGGGTGGATATGGATATGCAAAAAATATCACATTCGAGAAAATAACACTCGAAAATGTAAAAAACCCAATCATTATCGATCAACATTACACAGTTTATGCATATGGAAGGAAAAATAAGGGAAGGGAAATAAAAGTGAGCGATGTGACATATCGTAACGTTCATGGAACATCAGCAAATGAAAAGGCAATTACATTGGACTGTTCTCGAGCTCGTTGCACAAATATTATTATGGAAGAAGTGAACATTACGACATCAACTTCTGACGAAGAAGCCAAATCTTTTTGCCAAAATGCTGATGGAAAATCCACATCTACAGTTCCACCGGTGCCTTGTTTATCAAAATCTCAT

Coding sequence (CDS)

ATGAAGCTTCTTGTTTCCTTTGTTTTCCTCTTTTCCATTGCTGTTTTAAGCTCATTGACAGAATCAGCTTCGTCGTATTTCAATGTTCTTGATTATGGAGCAAATGGAAATGGCAAAAAAGATGATTCAGAAGCTTTTTTGAAAGCATGGAATGATGTATGTGCATCAACAGAAGACAACCCAACGTTACGTGTACCATCTGGAAAAACTTTCCTATTGAACCCTCTCAAATTCCAGGGTCCTTGCAAGTCCAAACGAATCAATTTTGATCTTGGTGGAAGTGTAATGGCTCCAACCAAGGATGAATGGTCCGGAGATGGTGTTGATAAATGGATCCAATTTTACGACATAGATTCTCTCACCGTTAAAGGAGATGGACGGTTTGATGGCCAAGGTTCTTCTTGGTGGGACAAAGATTGTCATAAACATTGCAAACGGCCAACGGCAATATTTTTCCACAACTGCAACGGTCTTCATCTAAGTAAAACGAAGCATGTTAATAGTGGCAAAAATCATATGTCCATAAATTGTTGCAATGATGTAATTATTTCAGATATTCATATTACTGCTCCTGATGAGAGTCCTAATACGGATGGAATTGATATTTCTGGATCAAAAAATGTTCGTATTCAAGATTCTTTTATAGGCACAGGTGATGATTGTGTTGCAATTAATGATGGATCTTCAAATATCGATATAACAGGTCTCACTTGTGGCCCCGGTCACGGCATAAGCATTGGGAGCTTAGGAAAAAACGGTGATTATAATGTTGTTGAAAATGTCTATGTAAGTGATTGTATCTTACAAGATACTCAAAACGGAGTTAGAATCAAGACTTGGGAGGGTGGATATGGATATGCAAAAAATATCACATTCGAGAAAATAACACTCGAAAATGTAAAAAACCCAATCATTATCGATCAACATTACACAGTTTATGCATATGGAAGGAAAAATAAGGGAAGGGAAATAAAAGTGAGCGATGTGACATATCGTAACGTTCATGGAACATCAGCAAATGAAAAGGCAATTACATTGGACTGTTCTCGAGCTCGTTGCACAAATATTATTATGGAAGAAGTGAACATTACGACATCAACTTCTGACGAAGAAGCCAAATCTTTTTGCCAAAATGCTGATGGAAAATCCACATCTACAGTTCCACCGGTGCCTTGTTTATCAAAATCTCAT

Protein sequence

MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSLTVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEEVNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH
Homology
BLAST of Cp4.1LG03g10420 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 7.7e-97
Identity = 195/391 (49.87%), Postives = 250/391 (63.94%), Query Frame = 0

Query: 11  FSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKT 70
           FS+  L   T  +S+  +V  +GA G+G  DDS+AFLKAW  VC+ T D   + VP+G T
Sbjct: 10  FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFV-VPAGMT 69

Query: 71  FLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSLTVKGDGRFDG 130
           F+L PLKFQG CKS  +   + G ++AP+K  W GD  D+WI F DI+ L ++GDG  +G
Sbjct: 70  FMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEING 129

Query: 131 QGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITA 190
           QGSSWW+   HK   RPTA+ F +CN L LS   H++S   H+ I+ CN V IS + I A
Sbjct: 130 QGSSWWE---HKG-SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINA 189

Query: 191 PDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLG 250
           P+ SPNTDGID+  S NV IQD  I TGDDC+AIN G+SNI I+G+ CGPGHGISIGSLG
Sbjct: 190 PESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLG 249

Query: 251 KNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHY 310
           K+G+   VENV V +C  + T NG RIKTW+GG GYA+ ITF  ITL+NV+NPIIIDQ Y
Sbjct: 250 KDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFY 309

Query: 311 T--VYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCS-RARCTNIIMEEVNITT-- 370
                   +  K   ++VS V + N  GTS +E  +   CS R  CT I + ++ I T  
Sbjct: 310 NGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETAS 369

Query: 371 STSDEEAKSFCQNADGKSTSTVPPVPCLSKS 397
           S S + A+  C N  G ST  VP + CL  S
Sbjct: 370 SGSGQVAQGQCLNVRGASTIAVPGLECLELS 392

BLAST of Cp4.1LG03g10420 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 7.2e-79
Identity = 161/419 (38.42%), Postives = 240/419 (57.28%), Query Frame = 0

Query: 4   LVSFVFLFSIAVLSSLTESASSYFNVL---------------------DYGANGNGKKDD 63
           LV+ + L  +A   +LT +A+S+ ++L                     ++GA GNG  DD
Sbjct: 9   LVTLLLLLVVASSLALTANANSFESLLQLPRRQSRSTRPRSERLLHVGNFGAKGNGVTDD 68

Query: 64  SEAFLKAWNDVCASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPT-KD 123
           ++AF  AW   C+S      L VP   T LL P+   GPCK+ R+   + G+++AP   D
Sbjct: 69  TKAFADAWKTACSSKVKTRIL-VPENYTCLLRPIDLSGPCKA-RLTLQISGTIIAPNDPD 128

Query: 124 EWSGDGVDKWIQFYDIDSLTVKGDGRFDGQGSSWWDKDC-HKH---CK-RPTAIFFHNCN 183
            W G    KW+ F+ +  LTV+G G  +G G  WW++ C H H   C+  PTA+ FH C 
Sbjct: 129 VWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSNPCRGAPTALTFHKCK 188

Query: 184 GLHLSKTKHVNSGKNHMSINCCNDVIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIG 243
            + +     ++S + H+++  C  V IS + + AP  SPNTDGI IS S+ + I ++ + 
Sbjct: 189 NMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIHISVSRGIVIDNTTVS 248

Query: 244 TGDDCVAINDGSSNIDITGLTCGPGHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVR 303
           TGDDC++I   S+ I I+ + CGPGHGISIGSLGK+  +  V ++ V   I+ DT NGVR
Sbjct: 249 TGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDITVDTAIISDTANGVR 308

Query: 304 IKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHG 363
           IKTW+GG G    I F  I + NV NPIIIDQ+Y        N+   I + ++++ +V G
Sbjct: 309 IKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQTSAISIENISFVHVRG 368

Query: 364 TSANEKAITLDCS-RARCTNIIMEEVNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLS 395
           TSA+++AI + CS  + C NI+++++++  S  D   +SFC  A G S+  V P PCLS
Sbjct: 369 TSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAYGSSSGQVYPPPCLS 425

BLAST of Cp4.1LG03g10420 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 6.1e-78
Identity = 169/389 (43.44%), Postives = 237/389 (60.93%), Query Frame = 0

Query: 15  VLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKTFLLN 74
           + S  T   +S  +V ++GA G+GK DD++AF KAW   C ST    T  VP GKT+LL 
Sbjct: 56  ISSDETTLEASTVSVSNFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKTYLLK 115

Query: 75  PLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDK--WIQFYDIDSLTVKG--DGRFDG 134
             +F+GPCKS R NF + G++ A TK     D  DK  W+   D+++L++ G   G  +G
Sbjct: 116 STRFRGPCKSLR-NFQILGTLSASTK---RSDYKDKNHWLILEDVNNLSIDGGSTGIING 175

Query: 135 QGSSWWDKDC----HKHC-KRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISD 194
            G +WW   C     K C K PTA+  +N   L++   +  N+ +  +SI  CN V +S+
Sbjct: 176 NGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEVSN 235

Query: 195 IHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGIS 254
           + ITAP +SPNTDGI I+ ++N+R+ +S IGTGDDC++I DG+ N+ I  LTCGPGHGIS
Sbjct: 236 VEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHGIS 295

Query: 255 IGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPII 314
           IGSLG +     V  + V      ++ NGVRIKT++GG G AKNI F+ I +ENVKNPII
Sbjct: 296 IGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNPII 355

Query: 315 IDQHYTVYAYGRKNKGRE----IKVSDVTYRNVHGTSANEKAITLDCS-RARCTNIIMEE 374
           IDQ Y       K+K  +    ++V +V Y+N+ GTSA + AITL+CS +  C  I++E 
Sbjct: 356 IDQDYC-----DKDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLEN 415

Query: 375 VNITTSTSDEEAKSFCQNADGKSTSTVPP 390
           V I   T+       C+NA+ K+  TV P
Sbjct: 416 VKIKGGTAS------CKNANVKNQGTVSP 428

BLAST of Cp4.1LG03g10420 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 291.2 bits (744), Expect = 1.8e-77
Identity = 158/364 (43.41%), Postives = 219/364 (60.16%), Query Frame = 0

Query: 22  SASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKTFLLNPLKFQGP 81
           S  +  +V D+GA G+G  DD++AF KAW D C+S      L VP  K +LL  + F GP
Sbjct: 80  SPDTDISVDDFGARGDG-TDDTKAFEKAWKDACSS---GSVLIVPENKNYLLKQITFSGP 139

Query: 82  CKSKRINFDLGGSVMAPT-KDEWSGDGVDKWIQFYDIDSLTVKGDGRFDGQGSSWWDKDC 141
           CKS  +   + G++ A + + +W G    +WI+F DI +LT++G G  +G G +WWD  C
Sbjct: 140 CKSD-LRVKIRGTIEASSDQSDWVGHNRKRWIEFEDISNLTLEGGGTSNGNGETWWDSSC 199

Query: 142 HKH----CK-RPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITAPDESP 201
            +     CK  PTA+ F +C  L +S     +S K H+S + C DVI S++ +TAP+ SP
Sbjct: 200 KRKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVIASNLMVTAPEHSP 259

Query: 202 NTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLGKNGDY 261
           NTDGI I+G++ + + +S IGTGDDC++I  GS  +  T +TCGPGHGISIGSLG     
Sbjct: 260 NTDGIHITGTQRIHVMNSVIGTGDDCISIESGSKMVIATNITCGPGHGISIGSLGDRNSE 319

Query: 262 NVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTVYAY 321
             V  V V    L DT NG+RIKTW+GG G AKNI F+ I + NV NPIIIDQ+Y     
Sbjct: 320 AHVSGVLVDGGNLFDTTNGLRIKTWQGGSGSAKNIKFQNIVMHNVTNPIIIDQYYCDSKD 379

Query: 322 GRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRAR-CTNIIMEEVNITTSTSDEEAKS 379
               +   +KVS+V Y N+ GTSA+E A+  DCS++  C   I+  +N+  +   E   S
Sbjct: 380 PCPEQESAVKVSNVAYMNIRGTSASEVAVKFDCSKSSPCQGYIVGNINLVGNGGKETTMS 437

BLAST of Cp4.1LG03g10420 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 283.1 bits (723), Expect = 4.8e-75
Identity = 154/379 (40.63%), Postives = 220/379 (58.05%), Query Frame = 0

Query: 22  SASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKTFLLNPLKFQGP 81
           S+   FNV  +GA  NG  DDS+AF+KAW   C+ST     +  P  + ++L  + F GP
Sbjct: 66  SSPRSFNVNTFGAKANG-NDDSKAFMKAWEAACSST-GIVYIVAPKNRDYMLKAVTFSGP 125

Query: 82  CKSKRINFDLGGSVMAPTKDEWSGDGVDK----WIQFYDIDSLTVKGDGRFDGQGSSWWD 141
           CKS  I F + G + A     W      K    WI F ++++L V+G GR DG G  WW 
Sbjct: 126 CKSSLIIFKIYGRIEA-----WENPSDYKERRHWIVFENVNNLRVEGGGRIDGNGHIWWP 185

Query: 142 KDCHKHCK-----RPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITAPD 201
           K C  + +      PTA+ F  CN L +S  +  N+ + H++   C +V   ++ +T+P 
Sbjct: 186 KSCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPA 245

Query: 202 ESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLGKN 261
           +SPNTDGI +SG++N+ IQDS + TGDDC++I  GS N+  TG+TCGPGHGISIGSLG++
Sbjct: 246 DSPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGED 305

Query: 262 GDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTV 321
                V NV V+   L  T NGVRIKTW+GG+G AKNI F+ I ++NV NPIII+Q Y  
Sbjct: 306 NSEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCD 365

Query: 322 YAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEE 381
                  +   ++VS+V Y+N+ GTS+   A+   CS+   C  I M+ V +   T  + 
Sbjct: 366 RVEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDV 425

Query: 382 AKSFCQNADGKSTSTVPPV 391
           +K+ C N    +   V P+
Sbjct: 426 SKASCSNVKLDTRGNVSPL 437

BLAST of Cp4.1LG03g10420 vs. NCBI nr
Match: XP_023526924.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 790 bits (2040), Expect = 2.10e-288
Identity = 388/397 (97.73%), Postives = 388/397 (97.73%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN
Sbjct: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
           TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND
Sbjct: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP
Sbjct: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYT         GREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYT---------GREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH
Sbjct: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 388

BLAST of Cp4.1LG03g10420 vs. NCBI nr
Match: XP_022979578.1 (probable polygalacturonase At3g15720 [Cucurbita maxima])

HSP 1 Score: 780 bits (2014), Expect = 2.71e-284
Identity = 378/397 (95.21%), Postives = 387/397 (97.48%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLLVSFVFLFS AVLSSLTES SSYFNVLDYGANGNGKKDDSEAFLKAWND CASTEDN
Sbjct: 1   MKLLVSFVFLFSTAVLSSLTESTSSYFNVLDYGANGNGKKDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTLRVP GKTFLLNPLKFQGPCKS+RINFDLGG VMAP+KDEWS DGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPFGKTFLLNPLKFQGPCKSERINFDLGGIVMAPSKDEWSNDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
           TVKGDGRFDGQGSSWWDKDCHKHCKRPTA+FFHNCNGLHL+KTKHVNSG+NHMSIN CND
Sbjct: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTALFFHNCNGLHLNKTKHVNSGRNHMSINNCND 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDI GLTCGP
Sbjct: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+GDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKDGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYT YAY RKNKGREIKVSDVTYR+VHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYTAYAYRRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNITTS+S+EEAKSFCQNADGKSTSTVPPVPCLSKSH
Sbjct: 361 VNITTSSSNEEAKSFCQNADGKSTSTVPPVPCLSKSH 397

BLAST of Cp4.1LG03g10420 vs. NCBI nr
Match: XP_022956242.1 (probable polygalacturonase At3g15720 [Cucurbita moschata] >KAG7018737.1 putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 776 bits (2004), Expect = 9.07e-283
Identity = 376/397 (94.71%), Postives = 385/397 (96.98%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKL VSFVFLF I VLSSLTESASSYFNVLDYGA+GNGK DDSEAFLKAWND CASTEDN
Sbjct: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGG VMAP+KDEWS DGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
           TVKGDG+FDGQGSSWWDKDCHKHCKRPTAIFFHNCN LHLSKTKHVNSG+NHMSIN CND
Sbjct: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           VIISDIHITAP+ESPNTDGIDISGSKNVRI DSFIGTGDDC+AINDGSSNIDI GLTCGP
Sbjct: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGKNGDYNVVENVYVS+CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYT YAYGRKNKGREIKVSDVTYR+VHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNITTSTSDEEAKSFCQNADG+STSTVPPVPCLSKSH
Sbjct: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 397

BLAST of Cp4.1LG03g10420 vs. NCBI nr
Match: XP_023552838.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 663 bits (1711), Expect = 3.99e-238
Identity = 312/397 (78.59%), Postives = 352/397 (88.66%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLL + +FLF + VL SLT SA SYFNVLDYGA GNGK DDSEAFLKAWND CA  EDN
Sbjct: 1   MKLLFAVIFLFYVTVLRSLTVSALSYFNVLDYGATGNGKTDDSEAFLKAWNDACAVIEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTLRVP GKT+LL PLKFQGPCKSK++NFDLGG++MAP+KDEW  DG DKW+QFY+IDSL
Sbjct: 61  PTLRVPFGKTYLLKPLKFQGPCKSKQVNFDLGGALMAPSKDEWPSDGEDKWVQFYNIDSL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
           TV+GDGRF+GQGSSWW+KDCH HC RPTA+FFHNCNGL LSK KH+NSGKNH+SIN CN+
Sbjct: 121 TVQGDGRFNGQGSSWWNKDCHNHCNRPTALFFHNCNGLRLSKMKHINSGKNHISINDCNN 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           V+ISDIHI+AP++SPNTDGIDIS SKNV IQ+S I TGDDC+AIN+GSSNI+I G+TCGP
Sbjct: 181 VVISDIHISAPEQSPNTDGIDISQSKNVLIQNSLIATGDDCIAINNGSSNINIVGITCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+GDYNVVEN+YV +C L+DTQNGVRIKTWEGGYGYA+NITFEKI LEN 
Sbjct: 241 GHGISIGSLGKDGDYNVVENIYVRNCHLKDTQNGVRIKTWEGGYGYARNITFEKIILENT 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQ+Y+ +AY RKN GREIKVS+VTYR VHGTSANE AI L+CSRARCTNIIM  
Sbjct: 301 KNPIIIDQYYSAFAYARKNMGREIKVSNVTYREVHGTSANENAIILNCSRARCTNIIMNN 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNI TST D EAKS CQNADG++ S +PPVPCLSKSH
Sbjct: 361 VNIRTSTPDTEAKSVCQNADGRAISVIPPVPCLSKSH 397

BLAST of Cp4.1LG03g10420 vs. NCBI nr
Match: XP_038888676.1 (probable polygalacturonase At3g15720 [Benincasa hispida])

HSP 1 Score: 613 bits (1581), Expect = 2.48e-218
Identity = 295/398 (74.12%), Postives = 341/398 (85.68%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLL + +F+F+I +L+    SASS FNVLDYGA GNGK DDSEAFLKAWNDVC +TED+
Sbjct: 1   MKLLFALIFIFTIDILTLSIVSASSNFNVLDYGAIGNGKADDSEAFLKAWNDVCGATEDS 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTL VP GKT+LLNPLKFQGPCKS ++NFDL G++MAP+KDEW  DG DKW+QFYDI+ L
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSNQVNFDLRGTLMAPSKDEWPSDGADKWVQFYDIEGL 120

Query: 121 TVKGDGRFDGQGSSWWDKDC-HKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCN 180
            +KG G+FDGQGS WW KDC  KHCKRPTA+FFHNCNGL + + KH+NSG+NH+SIN CN
Sbjct: 121 NLKGAGKFDGQGSLWW-KDCDRKHCKRPTALFFHNCNGLQVKEMKHINSGRNHISINDCN 180

Query: 181 DVIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCG 240
           DVI S+I I AP+ESPNTDGIDIS SKNV IQDSFI TGDDC+AIN+GSS+I+I G+TCG
Sbjct: 181 DVIFSNIQILAPEESPNTDGIDISKSKNVLIQDSFIATGDDCIAINEGSSDINIIGITCG 240

Query: 241 PGHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLEN 300
           PGHGISIGSLGK+G YNVVEN++VS+C+LQ TQNGVRIKTW+GGYGYAKNITFEKITL+N
Sbjct: 241 PGHGISIGSLGKDGAYNVVENIHVSNCLLQGTQNGVRIKTWQGGYGYAKNITFEKITLKN 300

Query: 301 VKNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIME 360
            KNPIIIDQ+YT YAY RK KG +IKVSDVTYR V+GTSANE AITL+CSRARCTNII++
Sbjct: 301 AKNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEDAITLNCSRARCTNIILD 360

Query: 361 EVNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
            VNI   T  +EAK+ CQN DGK  STVP V CLSKSH
Sbjct: 361 NVNIKMITLGKEAKAICQNVDGKFLSTVPIVSCLSKSH 397

BLAST of Cp4.1LG03g10420 vs. ExPASy TrEMBL
Match: A0A6J1IWN4 (probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111479259 PE=3 SV=1)

HSP 1 Score: 780 bits (2014), Expect = 1.31e-284
Identity = 378/397 (95.21%), Postives = 387/397 (97.48%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLLVSFVFLFS AVLSSLTES SSYFNVLDYGANGNGKKDDSEAFLKAWND CASTEDN
Sbjct: 1   MKLLVSFVFLFSTAVLSSLTESTSSYFNVLDYGANGNGKKDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTLRVP GKTFLLNPLKFQGPCKS+RINFDLGG VMAP+KDEWS DGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPFGKTFLLNPLKFQGPCKSERINFDLGGIVMAPSKDEWSNDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
           TVKGDGRFDGQGSSWWDKDCHKHCKRPTA+FFHNCNGLHL+KTKHVNSG+NHMSIN CND
Sbjct: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTALFFHNCNGLHLNKTKHVNSGRNHMSINNCND 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDI GLTCGP
Sbjct: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+GDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKDGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYT YAY RKNKGREIKVSDVTYR+VHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYTAYAYRRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNITTS+S+EEAKSFCQNADGKSTSTVPPVPCLSKSH
Sbjct: 361 VNITTSSSNEEAKSFCQNADGKSTSTVPPVPCLSKSH 397

BLAST of Cp4.1LG03g10420 vs. ExPASy TrEMBL
Match: A0A6J1GVS6 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111457994 PE=3 SV=1)

HSP 1 Score: 776 bits (2004), Expect = 4.39e-283
Identity = 376/397 (94.71%), Postives = 385/397 (96.98%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKL VSFVFLF I VLSSLTESASSYFNVLDYGA+GNGK DDSEAFLKAWND CASTEDN
Sbjct: 1   MKLFVSFVFLFFIVVLSSLTESASSYFNVLDYGADGNGKTDDSEAFLKAWNDACASTEDN 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGG VMAP+KDEWS DGVDKWIQFYDIDSL
Sbjct: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGIVMAPSKDEWSSDGVDKWIQFYDIDSL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
           TVKGDG+FDGQGSSWWDKDCHKHCKRPTAIFFHNCN LHLSKTKHVNSG+NHMSIN CND
Sbjct: 121 TVKGDGQFDGQGSSWWDKDCHKHCKRPTAIFFHNCNRLHLSKTKHVNSGRNHMSINSCND 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           VIISDIHITAP+ESPNTDGIDISGSKNVRI DSFIGTGDDC+AINDGSSNIDI GLTCGP
Sbjct: 181 VIISDIHITAPNESPNTDGIDISGSKNVRILDSFIGTGDDCIAINDGSSNIDIIGLTCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGKNGDYNVVENVYVS+CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV
Sbjct: 241 GHGISIGSLGKNGDYNVVENVYVSECILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQHYT YAYGRKNKGREIKVSDVTYR+VHGTSANEKAITLDCSRARCTNIIMEE
Sbjct: 301 KNPIIIDQHYTAYAYGRKNKGREIKVSDVTYRDVHGTSANEKAITLDCSRARCTNIIMEE 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNITTSTSDEEAKSFCQNADG+STSTVPPVPCLSKSH
Sbjct: 361 VNITTSTSDEEAKSFCQNADGESTSTVPPVPCLSKSH 397

BLAST of Cp4.1LG03g10420 vs. ExPASy TrEMBL
Match: A0A5D3CYS8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00190 PE=3 SV=1)

HSP 1 Score: 603 bits (1554), Expect = 1.55e-214
Identity = 286/397 (72.04%), Postives = 338/397 (85.14%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLL++ +  +SIA+LSS T  +SS FNVLDYGA GNGK DDS+AFLKAWNDVC +T+D+
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTL VP GKT+LL+PLKFQGPCKS ++ FDLGG++MAPTKDEW  DG  KW+QF D++ L
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
            V+G GRFDGQGS WW    HK CKRPTA+FFHNCNGL ++  KHVNS KNH+SIN CND
Sbjct: 121 NVQGGGRFDGQGSCWWKGCDHKQCKRPTALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           VI S +HI+AP++SPNTDGIDIS S N+ IQDSFIGTGDDCVAIN+GSSNI+I  +TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+G+YNVVENV+VS+C+L++TQNG+RIKTWEGGYGYAKNITFEKITL+N 
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQ+YT YAY RK KG +IKVSDVTYR V+GTSANE+AIT +CS+ARC+NII++ 
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNI  +   EEAK+ CQN DGK  S VPPV CLSKSH
Sbjct: 361 VNIKMTNPGEEAKAVCQNVDGKVLSAVPPVSCLSKSH 397

BLAST of Cp4.1LG03g10420 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 2.10e-211
Identity = 285/398 (71.61%), Postives = 339/398 (85.18%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLL + +FL+SIA+LSS T   SS FNVLDYGA GNGK DDS+AFLKAWNDVC +TE++
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTL VP GKT+LLNPLKFQGPCKSK+    LGG++MAPTKDEW  DG  +WIQF+DI+ L
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSKQ----LGGTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 121 TVKGDGRFDGQGSSWWDKDC-HKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCN 180
            ++G GRFDGQGS WW KDC HKHCKRPTA+FFHNCNGL +   KH+NS KNH+SIN C+
Sbjct: 121 NLQGGGRFDGQGSLWW-KDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCH 180

Query: 181 DVIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCG 240
           DVI S +HI+AP++SPNTDGIDIS S N+ I+DSF+ TGDDC+AIN+GSSNI+I G+TCG
Sbjct: 181 DVIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCG 240

Query: 241 PGHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLEN 300
           PGHGISIGSLGK+G+YNVVENV+VS+C+L+ TQNG+RIKTWEGGYGYAKNITFEKIT++N
Sbjct: 241 PGHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKN 300

Query: 301 VKNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIME 360
            KNPIIIDQ+Y+ YAY RK KG +IKVSDVTYR V+GTSANE AIT +CS+ARC+NII++
Sbjct: 301 AKNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILD 360

Query: 361 EVNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
            VNI  +   E+AK+ CQNADGK  S VPPV CLSKSH
Sbjct: 361 NVNIKMTNPGEKAKAVCQNADGKVLSAVPPVSCLSKSH 393

BLAST of Cp4.1LG03g10420 vs. ExPASy TrEMBL
Match: A0A1S3BYT5 (probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=3 SV=1)

HSP 1 Score: 580 bits (1495), Expect = 9.86e-206
Identity = 279/397 (70.28%), Postives = 331/397 (83.38%), Query Frame = 0

Query: 1   MKLLVSFVFLFSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDN 60
           MKLL++ +  +SIA+LSS T  +SS FNVLDYGA GNGK DDS+AFLKAWNDVC +T+D+
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 61  PTLRVPSGKTFLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSL 120
           PTL VP GKT+LL+PLKFQGPCKS ++ FDLGG++MAPTKDEW  DG  KW+QF D++ L
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 121 TVKGDGRFDGQGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCND 180
            V+G GRFDGQGS WW            A+FFHNCNGL ++  KHVNS KNH+SIN CND
Sbjct: 121 NVQGGGRFDGQGSCWWK-----------ALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 181 VIISDIHITAPDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGP 240
           VI S +HI+AP++SPNTDGIDIS S N+ IQDSFIGTGDDCVAIN+GSSNI+I  +TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 241 GHGISIGSLGKNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENV 300
           GHGISIGSLGK+G+YNVVENV+VS+C+L++TQNG+RIKTWEGGYGYAKNITFEKITL+N 
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 301 KNPIIIDQHYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCSRARCTNIIMEE 360
           KNPIIIDQ+YT YAY RK KG +IKVSDVTYR V+GTSANE+AIT +CS+ARC+NII++ 
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

Query: 361 VNITTSTSDEEAKSFCQNADGKSTSTVPPVPCLSKSH 397
           VNI  +   EEAK+ CQN DGK  S VPPV CLSKSH
Sbjct: 361 VNIKMTNPGEEAKAVCQNVDGKVLSAVPPVSCLSKSH 386

BLAST of Cp4.1LG03g10420 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 5.4e-98
Identity = 195/391 (49.87%), Postives = 250/391 (63.94%), Query Frame = 0

Query: 11  FSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKT 70
           FS+  L   T  +S+  +V  +GA G+G  DDS+AFLKAW  VC+ T D   + VP+G T
Sbjct: 10  FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFV-VPAGMT 69

Query: 71  FLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSLTVKGDGRFDG 130
           F+L PLKFQG CKS  +   + G ++AP+K  W GD  D+WI F DI+ L ++GDG  +G
Sbjct: 70  FMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEING 129

Query: 131 QGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITA 190
           QGSSWW+   HK   RPTA+ F +CN L LS   H++S   H+ I+ CN V IS + I A
Sbjct: 130 QGSSWWE---HKG-SRPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINA 189

Query: 191 PDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLG 250
           P+ SPNTDGID+  S NV IQD  I TGDDC+AIN G+SNI I+G+ CGPGHGISIGSLG
Sbjct: 190 PESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLG 249

Query: 251 KNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHY 310
           K+G+   VENV V +C  + T NG RIKTW+GG GYA+ ITF  ITL+NV+NPIIIDQ Y
Sbjct: 250 KDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFY 309

Query: 311 T--VYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCS-RARCTNIIMEEVNITT-- 370
                   +  K   ++VS V + N  GTS +E  +   CS R  CT I + ++ I T  
Sbjct: 310 NGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETAS 369

Query: 371 STSDEEAKSFCQNADGKSTSTVPPVPCLSKS 397
           S S + A+  C N  G ST  VP + CL  S
Sbjct: 370 SGSGQVAQGQCLNVRGASTIAVPGLECLELS 392

BLAST of Cp4.1LG03g10420 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 348.2 bits (892), Expect = 8.7e-96
Identity = 194/391 (49.62%), Postives = 248/391 (63.43%), Query Frame = 0

Query: 11  FSIAVLSSLTESASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKT 70
           FS+  L   T  +S+  +V  +GA G+G  DDS+AFLKAW  VC+ T D   + VP+G T
Sbjct: 9   FSLFFLQIFT--SSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGTGDGQFV-VPAGMT 68

Query: 71  FLLNPLKFQGPCKSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSLTVKGDGRFDG 130
           F+L PLKFQG CKS  +   + G ++AP+K  W GD  D+WI F DI+ L ++GDG  +G
Sbjct: 69  FMLQPLKFQGSCKSTPVFVQMLGKLVAPSKGNWKGD-KDQWILFTDIEGLVIEGDGEING 128

Query: 131 QGSSWWDKDCHKHCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITA 190
           QGSSWW+   HK   RPT   F +CN L LS   H++S   H+ I+ CN V IS + I A
Sbjct: 129 QGSSWWE---HKG-SRPT---FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINA 188

Query: 191 PDESPNTDGIDISGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLG 250
           P+ SPNTDGID+  S NV IQD  I TGDDC+AIN G+SNI I+G+ CGPGHGISIGSLG
Sbjct: 189 PESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLG 248

Query: 251 KNGDYNVVENVYVSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHY 310
           K+G+   VENV V +C  + T NG RIKTW+GG GYA+ ITF  ITL+NV+NPIIIDQ Y
Sbjct: 249 KDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFY 308

Query: 311 T--VYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDCS-RARCTNIIMEEVNITT-- 370
                   +  K   ++VS V + N  GTS +E  +   CS R  CT I + ++ I T  
Sbjct: 309 NGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETAS 368

Query: 371 STSDEEAKSFCQNADGKSTSTVPPVPCLSKS 397
           S S + A+  C N  G ST  VP + CL  S
Sbjct: 369 SGSGQVAQGQCLNVRGASTIAVPGLECLELS 388

BLAST of Cp4.1LG03g10420 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 9.6e-95
Identity = 179/362 (49.45%), Postives = 236/362 (65.19%), Query Frame = 0

Query: 27  FNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKR 86
           +NVL++ A G+G+ DDSEAFL+AW   C    D  TL +PSGKTFLL P  FQGPCKS  
Sbjct: 24  YNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQGPCKSSS 83

Query: 87  INFDLGGSVMAPT-KDEWSGDGVDKWIQFYDIDSLTVKGDGRFDGQGSSWWDKDCHKHCK 146
           I   L G+++AP+ K  WS      WI+F  +  L + G G  D +GSS+W+ +  K  +
Sbjct: 84  IKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELNL-KASQ 143

Query: 147 RPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITAPDESPNTDGIDISGS 206
           RPTA+    C+ L ++    ++S KNH+SI  CN V IS+I++ AP+ SPNTDGIDIS S
Sbjct: 144 RPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPNTDGIDISDS 203

Query: 207 KNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLGKNGDYNVVENVYVSD 266
            N+ I DS I TGDDC+AIN GSSNI+ITG+ CGPGHGIS+GSLG  G    V +V V+ 
Sbjct: 204 TNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEAKVSDVQVTH 263

Query: 267 CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTVYAYGRKNKGREIK 326
           C    T NG RIKTW GG GYA+NI+F  ITL N KNPIIIDQHY     GR  +   + 
Sbjct: 264 CTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHY--IDKGRLTEESAVA 323

Query: 327 VSDVTYRNVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEEAKSFCQNADGKST 386
           +S+V + +  GTS+N+ AITL CS    C +++M+ ++IT +    + K  CQ  DG+S+
Sbjct: 324 ISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITMANGG-KPKVNCQYVDGESS 381

BLAST of Cp4.1LG03g10420 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 4.9e-91
Identity = 171/360 (47.50%), Postives = 231/360 (64.17%), Query Frame = 0

Query: 27  FNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKTFLLNPLKFQGPCKSKR 86
           +NVL +GA G+G+ DDS AFL+AWN  C   E+  TL +PSGKT+LL P++F+GPCKS  
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 87  INFDLGGSVMAPTK-DEWSGDGVDKWIQFYDIDSLTVKGDGRFDGQGSSWWDKDCHKHCK 146
           I   L G ++AP+    WS      WI F  +  L + G G  +G+GSS+W+        
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWE-------- 172

Query: 147 RPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITAPDESPNTDGIDISGS 206
              A+   NCN L ++    ++S K+H+SI  C+ V IS I+I AP+ SPNTDGIDIS S
Sbjct: 173 ---ALHICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYS 232

Query: 207 KNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLGKNGDYNVVENVYVSD 266
            NV I DS I TGDDC+AIN GSS+I+IT + CGPGHGIS+GSLG +G+   V +VYV+ 
Sbjct: 233 TNVNIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQ 292

Query: 267 CILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQHYTVYAYGRKNKGREIK 326
           C    T NG RIKTW+GG GYA+NI+FE ITL NV+NPIIIDQ YT        K   + 
Sbjct: 293 CTFNKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVA 352

Query: 327 VSDVTYRNVHGTSANEKAITLDCSR-ARCTNIIMEEVNITTSTSDEEAKSFCQNADGKST 385
           +S V Y    GT+ NE AI L CS    C +++++++ +T   + E+ K  C+N +G+S+
Sbjct: 353 ISSVKYVGFQGTTLNEDAIMLKCSAITYCKDMVIDDIEVTME-NGEKPKVECENVEGESS 400

BLAST of Cp4.1LG03g10420 vs. TAIR 10
Match: AT5G17200.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.7e-89
Identity = 169/381 (44.36%), Postives = 234/381 (61.42%), Query Frame = 0

Query: 23  ASSYFNVLDYGANGNGKKDDSEAFLKAWNDVCASTEDNPTLRVPSGKTFLLNPLKFQGPC 82
           ++ + +V  +GA G+GK DD++AFLKAW  VC     N  + VP GKTF+L PL F GPC
Sbjct: 35  SNGFISVTSFGAIGDGKTDDTKAFLKAWEAVCKGGH-NRKILVPQGKTFMLKPLTFIGPC 94

Query: 83  KSKRINFDLGGSVMAPTKDEWSGDGVDKWIQFYDIDSLTVKGDGRFDGQGSSWWDKDCHK 142
           KS  I+  + G+++AP    W       WI F  I+ L V G G  DG+GS WW    + 
Sbjct: 95  KSSTISLSIRGNLVAPGY-TWYAGRYTTWISFDSINGLVVTGGGTIDGRGSLWWGNVNN- 154

Query: 143 HCKRPTAIFFHNCNGLHLSKTKHVNSGKNHMSINCCNDVIISDIHITAPDESPNTDGIDI 202
              RP A+ F+NCNGL +S  +H+NS +NH+ ++C  ++ +  + +TAP +SPNTDGIDI
Sbjct: 155 ---RPCAMHFNNCNGLRISNLRHLNSPRNHVGLSCSQNIEVRGLRMTAPGDSPNTDGIDI 214

Query: 203 SGSKNVRIQDSFIGTGDDCVAINDGSSNIDITGLTCGPGHGISIGSLGKNGDYNVVENVY 262
           S    V I DS I TGDDC+AIN GSS+I+ITG+ CGPGHGIS+GSLG  GD+  VE V 
Sbjct: 215 SNCIGVHIHDSVIATGDDCIAINSGSSHINITGIFCGPGHGISVGSLGVTGDFETVEEVR 274

Query: 263 VSDCILQDTQNGVRIKTWEGGYGYAKNITFEKITLENVKNPIIIDQ-------------- 322
           V +C    TQNGVRIKT++ G GYA+ I+FE I +   +NPIIIDQ              
Sbjct: 275 VKNCTFTKTQNGVRIKTYQNGSGYARKISFEDINMVASENPIIIDQTYHNGGTNGGISKS 334

Query: 323 -------HYTVYAYGRKNKGREIKVSDVTYRNVHGTSANEKAITLDC-SRARCTNIIMEE 382
                  H T         G+ +KV+DV Y  + G+SA+++ ITL+C +   C++I+M+ 
Sbjct: 335 SSSYQNCHLTAKQRTPSGNGKGVKVTDVRYARIRGSSASDQDITLNCDADLGCSDIVMDN 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LW077.7e-9749.87Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q94AJ57.2e-7938.42Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
O231476.1e-7843.44Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1[more]
Q020961.8e-7743.41Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1[more]
Q9SFB74.8e-7540.63Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023526924.12.10e-28897.73probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo][more]
XP_022979578.12.71e-28495.21probable polygalacturonase At3g15720 [Cucurbita maxima][more]
XP_022956242.19.07e-28394.71probable polygalacturonase At3g15720 [Cucurbita moschata] >KAG7018737.1 putative... [more]
XP_023552838.13.99e-23878.59probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo][more]
XP_038888676.12.48e-21874.12probable polygalacturonase At3g15720 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1IWN41.31e-28495.21probable polygalacturonase At3g15720 OS=Cucurbita maxima OX=3661 GN=LOC111479259... [more]
A0A6J1GVS64.39e-28394.71probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114579... [more]
A0A5D3CYS81.55e-21472.04Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A0A0KZ952.10e-21171.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1[more]
A0A1S3BYT59.86e-20670.28probable polygalacturonase At3g15720 OS=Cucumis melo OX=3656 GN=LOC103494869 PE=... [more]
Match NameE-valueIdentityDescription
AT3G15720.15.4e-9849.87Pectin lyase-like superfamily protein [more]
AT3G15720.28.7e-9649.62Pectin lyase-like superfamily protein [more]
AT4G35670.19.6e-9549.45Pectin lyase-like superfamily protein [more]
AT5G27530.14.9e-9147.50Pectin lyase-like superfamily protein [more]
AT5G17200.12.7e-8944.36Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 228..248
e-value: 4500.0
score: 2.6
coord: 205..226
e-value: 240.0
score: 10.5
coord: 258..279
e-value: 12.0
score: 14.9
coord: 287..308
e-value: 1000.0
score: 7.2
coord: 178..204
e-value: 16.0
score: 14.4
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 61..380
e-value: 9.6E-96
score: 320.6
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 235..248
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 13..393
e-value: 1.7E-119
score: 401.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 378..397
NoneNo IPR availablePANTHERPTHR31375:SF232SUBFAMILY NOT NAMEDcoord: 8..393
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 8..393
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 13..393

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g10420.1Cp4.1LG03g10420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity