Cp4.1LG03g08620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g08620
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	ribosomal RNA small subunit methyltransferase NEP1
Location	Cp4.1LG03: 2597644 .. 2600800 (-)
RNA-Seq Expression	Cp4.1LG03g08620
Synteny	Cp4.1LG03g08620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCCCAGCTGCAGGGCACGCTTTAAAGTTTCAATGACAAAACCATATCGAGTTCAAGGGTTTCAGCGCTAAGCTTCGGCTACAACCCTCTCTTCGCGCCTACTGTTAAGCGAGTTCGAGCTCAAAGAAGAACAATGGTTCGACCGTTTGCGGCGAAAGGGAAGAAGAGAAAGAAGACGGAAAGATACGACAGAGACGATGATGGCGAAGAATCGACCGCTTCGGCCAAGAAGGCTATGGTGGAGAACGAACCTGATGAACAACCAGAGAATGAAGCAGCCGAAGAAACCTTCCACGAGCTTGAAGGTATTCCAATCGTTCCGAAAGACCCCAAGAGTGATAGTAAGGCTGGTGTCATCTTCGTCCTCGAACGAGCTTCTCTGGAGGTCGCCAAAGTTGGGAAGGTAGGTATTCTCTTTTTTATGCATCGTTTTTTGGCTTGCTGCTTTGGAGTTTTAGGATTTAATTCAATTATGGCGTTTAACTGAGCTTTTTTTATGCGTTTTTGAAGTTTGAACTTACTATTTACTTTCCATTCTCTGTTTTTGTAAACTGGATTTCAATTTTCGTGGTTTTAGAATTACCAGCTGTTGAATTCCGATGACCACTCCAATTTTTTGCGGAGAAACAATAGAAATCCTGGGGATTACAGACCCGACATCCTGCATCAAGTAAGCAGCTGTAGCTCACTGTTGTTTTTCCAATTGGATAGGAGAGAGCGAATTGTTTATGTTGATATTTGAGTACCATAAGTGATATTAAATATAAATGATTCAGGCTCTCTTGGCGATTTTCGATAGCCGCATTGCAAAGGCTGGGAGGTTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATCAAACCATATGTTCGTCTTCCAAGGACGCAGAAGCGGTTTTATGGTGTTATGTGTAAGTTGATTTTGTTGTTCTAATTTGGCTGAACATCTATCGAATTGGAGTCTGTTTTGAATCCTTTTCATTGGCGTTTTGCCAAATACTTTGCAGTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGCAAGCGTGAGAAGCTTTTCAGGGTGATAAAAAACCCTGTAACACAGTATTTACCTTCCAATTCTCGTAAAATGGGTAAGCCTATATAATGCTGTTTCCCCATTGTGAGTTTTGGGGTTTGGTAGAAACTTTTCGTAATGTTTTCTTGCATATCAGGCTTCTCGCATAGTTCAGACAAGTTGGTCAAGGTGCGAACTTATCTGGACGCTGTTGAAGATGATGTAGACCTTGTTTTTGTGGTATCTTTAAACTCCCTACTGGAAATTTCACTTTTACATGCTTCATTGTTATGTTTATACATATGACAACTCCCCCTTACTTCCCTTTACTGCAATGTGTATTAGGTGGGTGCAATGGCTCATGGGAAAATTGAGACTGGTTACACCGATGATCTTTTAGCAAGTAAGTTCGTATTCTTTCGTGTTTATTTCAAGTAATTGGATAGGAGGAAATGACTACATTAGTACATTTACTTTTAACTTCAGAAGATTTTTGAAGGATGAAGTTTTAATATCATTTACTTTCAAGGACGTCTTCTAAATTGTAGCCAAGCATAAGCATTGAGATAGAAGGGCTTTTTAAGTTTTGACCATTTGAAAGCACTTCCAAAGATATCATAGAGTGGTTTAAGCATTACATTTGTGAAAAATTGACTAGTTGGCAAATTGCTCGAACTTTTACTGTTATTTCTGGGCATTATTATCTTGACTCTGTTTCTTATCCGAAAAGAAAAGAGATTATCTTGGCCGTGCAATCCTGAATTGTTTGACGAATTTTTGTGTTATATTTGCAGTTTCTGAATATCCTCTCAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGATAAATGGAATGTAGGGTGAACCATACCTACAACTATGCATCCAGTTAAGTCTGATAATCAGTGTCTCAACTCTGTTGTTTCATATTTATTAGTCATTATTTCAGAGAATCTGCCTGCAGATGTCTTAGGCGTATTAAACCTTACCATAGCTATTTCTGTGTAGCGAATTCTTAGTCGATTATGTTACCAATGCCTCTTCTTCTAAATATAATTGTAAGCAGTTGATTTCTAGTTCTTAAAAGTTCGATCAGAAGTGAAGTTCTTTGCTGTGCGTTAGAATATCAATTGTGAAAGGCAAATATGTTCACTTTTTCATGCCCAACATGAATAAGTTTGAAGTTGAAATCGTACAGTTTATTGATGTGTTTAATCTCTCAAGGTGAATAAGTTACAAATTTAGTTTTCAAATGGCAAATAGTTCTAATTATGCTTCTAAATTTTAAATAATCCCTAGTTTTTGCGATTAACATTGCTGAGTTTACCTGATATGATGTGATGCTAGATGAATAAATATGAGCCAGAAAGGAAGTAATTTATTGCAAGGAGGCTATTTTCTACACATATTAGACCAATAAGACGATCCCTTTTGACAACCAGGCAGTAACAAGTAGGATGTTGCCACTATGCATTCTTGAAACAATGAAATGTCAAAAGTATAGTTTCAAATATGAAGAGTATTTTTTTTGTAATTAAACCTAGAAGATAAATAGAAACAATTTGTTGTTTCTCGAGCTATTACAAAAGGAATATATACACGATGTAAACAGTCAATGAGGTTACAACCTAGTAAAAGAAATAAAGATACAGTTTTAGCTGATGTACAAAGAAACATGTACAAACAAGGTGCATTGATGGATGAATGAATGAGGACTTAGACTTGAGCTAAAGGGTTAGTAAAATTTAACATTATGGATACACATGAATAAGTATTTACCTTCATAGTCCTTGTACAGAAGCAAGCTGCTTCCTAAGATTAAGGTTCTCGATACATTCAGAAGCCAACTTTTTCTTCAAGTCTGCAGTAGCCTTCTCATACTTTCTATCCATAAGATCAGACTCATCCACTGCTCCCTGATATCTAATGCGGAATGCATCCAACTTTGCTTGAGTTCCTCGCAGCCTCATTCGAAGCTTAGCCTTCTCTTTGCCGTTCTGCACGGCATCCTGTTTGCAAGCATGTTAGATTTCCCTTGAACAACGTAAGATAATAATGGTAGATACATATGCTGTCTTATTTTATGCTCGCAAAG

mRNA sequence

AGCCCAGCTGCAGGGCACGCTTTAAAGTTTCAATGACAAAACCATATCGAGTTCAAGGGTTTCAGCGCTAAGCTTCGGCTACAACCCTCTCTTCGCGCCTACTGTTAAGCGAGTTCGAGCTCAAAGAAGAACAATGGTTCGACCGTTTGCGGCGAAAGGGAAGAAGAGAAAGAAGACGGAAAGATACGACAGAGACGATGATGGCGAAGAATCGACCGCTTCGGCCAAGAAGGCTATGGTGGAGAACGAACCTGATGAACAACCAGAGAATGAAGCAGCCGAAGAAACCTTCCACGAGCTTGAAGGTATTCCAATCGTTCCGAAAGACCCCAAGAGTGATAGTAAGGCTGGTGTCATCTTCGTCCTCGAACGAGCTTCTCTGGAGGTCGCCAAAGTTGGGAAGAATTACCAGCTGTTGAATTCCGATGACCACTCCAATTTTTTGCGGAGAAACAATAGAAATCCTGGGGATTACAGACCCGACATCCTGCATCAAGCTCTCTTGGCGATTTTCGATAGCCGCATTGCAAAGGCTGGGAGGTTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATCAAACCATATGTTCGTCTTCCAAGGACGCAGAAGCGGTTTTATGGTGTTATGTTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGCAAGCGTGAGAAGCTTTTCAGGGTGATAAAAAACCCTGTAACACAGTATTTACCTTCCAATTCTCGTAAAATGGGCTTCTCGCATAGTTCAGACAAGTTGGTCAAGGTGCGAACTTATCTGGACGCTGTTGAAGATGATGTAGACCTTGTTTTTGTGGTGGGTGCAATGGCTCATGGGAAAATTGAGACTGGTTACACCGATGATCTTTTAGCAATTTCTGAATATCCTCTCAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGATAAATGGAATGTAGGGTGAACCATACCTACAACTATGCATCCAATGAATAAATATGAGCCAGAAAGGAAGTAATTTATTGCAAGGAGGCTATTTTCTACACATATTAGACCAATAAGACGATCCCTTTTGACAACCAGGCAGTAACAAGTAGGATGTTGCCACTATGCATTCTTGAAACAATGAAATGTCAAAAGTATAGTTTCAAATATGAAGAGTATTTTTTTTGTAATTAAACCTAGAAGATAAATAGAAACAATTTGTTGTTTCTCGAGCTATTACAAAAGGAATATATACACGATGTAAACAGTCAATGAGGTTACAACCTAGTAAAAGAAATAAAGATACAGTTTTAGCTGATGTACAAAGAAACATGTACAAACAAGGTGCATTGATGGATGAATGAATGAGGACTTAGACTTGAGCTAAAGGGTTAGTAAAATTTAACATTATGGATACACATGAATAAGTATTTACCTTCATAGTCCTTGTACAGAAGCAAGCTGCTTCCTAAGATTAAGGTTCTCGATACATTCAGAAGCCAACTTTTTCTTCAAGTCTGCAGTAGCCTTCTCATACTTTCTATCCATAAGATCAGACTCATCCACTGCTCCCTGATATCTAATGCGGAATGCATCCAACTTTGCTTGAGTTCCTCGCAGCCTCATTCGAAGCTTAGCCTTCTCTTTGCCGTTCTGCACGGCATCCTGTTTGCAAGCATGTTAGATTTCCCTTGAACAACGTAAGATAATAATGGTAGATACATATGCTGTCTTATTTTATGCTCGCAAAG

Coding sequence (CDS)

ATGGTTCGACCGTTTGCGGCGAAAGGGAAGAAGAGAAAGAAGACGGAAAGATACGACAGAGACGATGATGGCGAAGAATCGACCGCTTCGGCCAAGAAGGCTATGGTGGAGAACGAACCTGATGAACAACCAGAGAATGAAGCAGCCGAAGAAACCTTCCACGAGCTTGAAGGTATTCCAATCGTTCCGAAAGACCCCAAGAGTGATAGTAAGGCTGGTGTCATCTTCGTCCTCGAACGAGCTTCTCTGGAGGTCGCCAAAGTTGGGAAGAATTACCAGCTGTTGAATTCCGATGACCACTCCAATTTTTTGCGGAGAAACAATAGAAATCCTGGGGATTACAGACCCGACATCCTGCATCAAGCTCTCTTGGCGATTTTCGATAGCCGCATTGCAAAGGCTGGGAGGTTGAAAGTTGTTTATGTGAAAACTGAGAAAGGTTTATTAATTGAAATCAAACCATATGTTCGTCTTCCAAGGACGCAGAAGCGGTTTTATGGTGTTATGTTGCAATTGCTACAAAAGTTAAGCATCACAGCTGCGGGCAAGCGTGAGAAGCTTTTCAGGGTGATAAAAAACCCTGTAACACAGTATTTACCTTCCAATTCTCGTAAAATGGGCTTCTCGCATAGTTCAGACAAGTTGGTCAAGGTGCGAACTTATCTGGACGCTGTTGAAGATGATGTAGACCTTGTTTTTGTGGTGGGTGCAATGGCTCATGGGAAAATTGAGACTGGTTACACCGATGATCTTTTAGCAATTTCTGAATATCCTCTCAGTGCTAGTTGCTGTATTGCGGATATTTGCAAAGATTTGGCAGATAAATGGAATGTAGGGTGA

Protein sequence

MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIPIVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG

Homology

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BLAST of Cp4.1LG03g08620 vs. ExPASy Swiss-Prot
Match: O35130 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=Emg1 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 9.6e-46
Identity = 100/212 (47.17%), Postives = 137/212 (64.62%), Query Frame = 0


Query: 70  SKAG---VIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILHQALLAI 129
           SK G   +I VLE ASLE  KVGK Y+LLN D H + L +N R+PG+ RPDI HQ+LL +
Sbjct: 36  SKCGGRRLIVVLEGASLETVKVGKTYELLNCDRHKSMLLKNGRDPGEVRPDITHQSLLML 95

Query: 130 FDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREK 189
            DS + +AG L+ VY+ T+K +LIE+ P  R+PRT  RF G+M+QLL KLS+ AA   +K
Sbjct: 96  MDSPLNRAGLLQ-VYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQK 155

Query: 190 LFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAHGKIETG 249
           L +VIKNPV+ + P    K+G S S + +  +R   + V     +VFVVGA AHGK+   
Sbjct: 156 LLKVIKNPVSDHFPVGCMKIGTSFSVEDISDIR---ELVPSSDPVVFVVGAFAHGKVSVE 215

Query: 250 YTDDLLAISEYPLSASCCIADICKDLADKWNV 279
           YT+ +++IS YPLSA+   A +     + W V
Sbjct: 216 YTEKMVSISNYPLSAALTCAKVTTAFEEVWGV 243

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy Swiss-Prot
Match: Q92979 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EMG1 PE=1 SV=4)

HSP 1 Score: 184.5 bits (467), Expect = 1.6e-45
Identity = 106/241 (43.98%), Postives = 149/241 (61.83%), Query Frame = 0


Query: 43  QPENEAAEETFHELEGIPIVPKDPK--SDSKAG---VIFVLERASLEVAKVGKNYQLLNS 102
           +P   +  E   + + +P  PK P+  + +K G   +I VLE ASLE  KVGK Y+LLN 
Sbjct: 9   KPRERSGGEQAQDWDALP--PKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNC 68

Query: 103 DDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVR 162
           D H + L +N R+PG+ RPDI HQ+LL + DS + +AG L+ VY+ T+K +LIE+ P  R
Sbjct: 69  DKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQ-VYIHTQKNVLIEVNPQTR 128

Query: 163 LPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVK 222
           +PRT  RF G+M+QLL KLS+ AA   +KL +VIKNPV+ + P    K+G S S   +  
Sbjct: 129 IPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSD 188

Query: 223 VRTYLDAVEDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLADKWN 279
           VR   + V     +VFVVGA AHGK+   YT+ +++IS YPLSA+   A +     + W 
Sbjct: 189 VR---ELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWG 243

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy Swiss-Prot
Match: Q9XX15 (Ribosomal RNA small subunit methyltransferase nep-1 OS=Caenorhabditis elegans OX=6239 GN=Y39A1A.14 PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.1e-44
Identity = 98/214 (45.79%), Postives = 135/214 (63.08%), Query Frame = 0


Query: 65  DPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILHQALL 124
           D + + K  +  VLE  SLE AKVG  Y +L+SD H+NFLR+  ++P DYRPDILHQ LL
Sbjct: 19  DNQLEDKKILYVVLEGCSLETAKVGGEYAILSSDKHANFLRKQKKDPADYRPDILHQCLL 78

Query: 125 AIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKR 184
            + DS + +AG+L+ V+ +T K +L+++ P  R+PRT  RF G+M+QLL KLSI AA   
Sbjct: 79  NLLDSPLNRAGKLR-VFFRTSKNVLVDVSPQCRIPRTFDRFCGLMVQLLHKLSIRAAETT 138

Query: 185 EKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAHGKIE 244
           +KL  V+KNPV+ +LP  SRKM  S +  +L  +   L A E D  LV ++G +A GKI 
Sbjct: 139 QKLMSVVKNPVSNHLPVGSRKMLMSFNVPELT-MANKLVAPETDEPLVLIIGGIARGKIV 198

Query: 245 TGYTDDLLAISEYPLSASCCIADICKDLADKWNV 279
             Y D    IS YPLSA+   A +   L + W +
Sbjct: 199 VDYNDSETKISNYPLSAALTCAKVTSGLEEIWGI 230

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy Swiss-Prot
Match: Q06287 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EMG1 PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.1e-44
Identity = 100/231 (43.29%), Postives = 140/231 (60.61%), Query Frame = 0


Query: 61  IVPKDP-----KSDSKAGVIFVLERASLEVAKVGKN------YQLLNSDDHSNFLRRNNR 120
           +VP+ P     K      +I VL  ASLE  K+  N      Y LLN DDH   L++  R
Sbjct: 23  LVPQAPPVLTSKDKITKRMIVVLAMASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGR 82

Query: 121 NPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVM 180
           +  + RPDI HQ LL + DS I KAG+L+ VY++T +G+LIE+ P VR+PRT KRF G+M
Sbjct: 83  DISEARPDITHQCLLTLLDSPINKAGKLQ-VYIQTSRGILIEVNPTVRIPRTFKRFSGLM 142

Query: 181 LQLLQKLSITAAGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDV 240
           +QLL KLSI +    EKL +VIKNP+T +LP+  RK+  S  +  +++V+ Y++ ++DD 
Sbjct: 143 VQLLHKLSIRSVNSEEKLLKVIKNPITDHLPTKCRKVTLSFDA-PVIRVQDYIEKLDDDE 202

Query: 241 DLVFVVGAMAHGK--IETGYTDDLLAISEYPLSASCCIADICKDLADKWNV 279
            +   VGAMA GK      Y D+ + +S YPLSAS   +  C    D WN+
Sbjct: 203 SICVFVGAMARGKDNFADEYVDEKVGLSNYPLSASVACSKFCHGAEDAWNI 251

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy Swiss-Prot
Match: Q10107 (Ribosomal RNA small subunit methyltransferase mra1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mra1 PE=1 SV=2)

HSP 1 Score: 173.3 bits (438), Expect = 3.8e-42
Identity = 110/261 (42.15%), Postives = 147/261 (56.32%), Query Frame = 0


Query: 20  RDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIPIVPKDPKSDSKAGVIFVLE 79
           + D+ +E    A    V+  P   P       T H +  IP         +   +I VL+
Sbjct: 99  KSDEEKEDPNGASSKTVKARP--LPAGSVHRVTTH-MAPIPARSIGSHDTTTQRLIVVLD 158

Query: 80  RASLEVAKVGK----NYQLLNSDDHSNFLRRNNRNPGDYRPDILHQALLAIFDSRIAKAG 139
           +A LE+ KVGK     YQLLN DDH   L++ NRN    RPDI HQ LL + DS + KAG
Sbjct: 159 QACLEIYKVGKAKDAKYQLLNCDDHQGILKKLNRNIAQARPDITHQCLLTLLDSPLNKAG 218

Query: 140 RLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITAAGKREKLFRVIKNPV 199
           RL+ VY+ T K +LIE+ P VR+PRT KRF G+M+QLL KLSI +    EKL +VIKNPV
Sbjct: 219 RLQ-VYIHTAKKVLIEVNPSVRIPRTFKRFSGLMVQLLHKLSIRSVNGNEKLLKVIKNPV 278

Query: 200 TQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAHG--KIETGYTDDLLA 259
           T YLP N RK   S  +   V  R YL+ ++ +  +   +GAMAHG      G+ D+ ++
Sbjct: 279 TDYLPPNCRKATLSFDA-PTVPPRKYLETLQPNQSVCIAIGAMAHGPDDFSDGWVDEKIS 338

Query: 260 ISEYPLSASCCIADICKDLAD 275
           IS+YPLSAS   +     + D
Sbjct: 339 ISDYPLSASIACSKFLHSMED 354

[Close]

BLAST of Cp4.1LG03g08620 vs. NCBI nr
Match: XP_023527836.1 (ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 542 bits (1397), Expect = 3.13e-194
Identity = 279/279 (100.00%), Postives = 279/279 (100.00%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

[Close]

BLAST of Cp4.1LG03g08620 vs. NCBI nr
Match: KAG7017643.1 (Ribosomal RNA small subunit methyltransferase NEP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540 bits (1392), Expect = 1.81e-193
Identity = 278/279 (99.64%), Postives = 278/279 (99.64%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEE FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

[Close]

BLAST of Cp4.1LG03g08620 vs. NCBI nr
Match: XP_022935622.1 (ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita moschata] >KAG6580885.1 Ribosomal RNA small subunit methyltransferase NEP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 538 bits (1387), Expect = 1.05e-192
Identity = 277/279 (99.28%), Postives = 277/279 (99.28%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKK ERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEE FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

[Close]

BLAST of Cp4.1LG03g08620 vs. NCBI nr
Match: XP_022983847.1 (ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita maxima])

HSP 1 Score: 538 bits (1386), Expect = 1.49e-192
Identity = 277/279 (99.28%), Postives = 277/279 (99.28%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKKTERYDRDDDGEES ASAKKAMVENEPDEQPENEAAEE FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKTERYDRDDDGEESIASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

[Close]

BLAST of Cp4.1LG03g08620 vs. NCBI nr
Match: XP_008443268.1 (PREDICTED: ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo] >KAA0053876.1 ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo var. makuwa] >TYK25528.1 ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo var. makuwa])

HSP 1 Score: 494 bits (1272), Expect = 3.05e-175
Identity = 252/279 (90.32%), Postives = 266/279 (95.34%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKK E+YDRD+DGEES++ AKK MVENEPDE+P     +E FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEP----PKEDFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPK+D+ AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKTDNNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVK+R YLDAV+DDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKMRNYLDAVKDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 241 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 275

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy TrEMBL
Match: A0A6J1F634 (ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita moschata OX=3662 GN=LOC111442430 PE=3 SV=1)

HSP 1 Score: 538 bits (1387), Expect = 5.07e-193
Identity = 277/279 (99.28%), Postives = 277/279 (99.28%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKK ERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEE FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy TrEMBL
Match: A0A6J1J0G6 (ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita maxima OX=3661 GN=LOC111482341 PE=3 SV=1)

HSP 1 Score: 538 bits (1386), Expect = 7.20e-193
Identity = 277/279 (99.28%), Postives = 277/279 (99.28%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKKTERYDRDDDGEES ASAKKAMVENEPDEQPENEAAEE FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKTERYDRDDDGEESIASAKKAMVENEPDEQPENEAAEENFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG
Sbjct: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy TrEMBL
Match: A0A5D3DPQ6 (Ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006800 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 1.47e-175
Identity = 252/279 (90.32%), Postives = 266/279 (95.34%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKK E+YDRD+DGEES++ AKK MVENEPDE+P     +E FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEP----PKEDFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPK+D+ AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKTDNNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVK+R YLDAV+DDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKMRNYLDAVKDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 241 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 275

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy TrEMBL
Match: A0A1S3B7L0 (ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo OX=3656 GN=LOC103486891 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 1.47e-175
Identity = 252/279 (90.32%), Postives = 266/279 (95.34%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKK E+YDRD+DGEES++ AKK MVENEPDE+P     +E FHELEGIP
Sbjct: 1   MVRPFAAKGKKRKKAEKYDRDEDGEESSSPAKKVMVENEPDEEP----PKEDFHELEGIP 60

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           IVPKDPK+D+ AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 61  IVPKDPKTDNNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 120

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVK+R YLDAV+DDVDLVFVVGAMAH
Sbjct: 181 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKMRNYLDAVKDDVDLVFVVGAMAH 240

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 241 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 275

[Close]

BLAST of Cp4.1LG03g08620 vs. ExPASy TrEMBL
Match: A0A0A0LFI3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G821550 PE=3 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 4.73e-175
Identity = 252/279 (90.32%), Postives = 266/279 (95.34%), Query Frame = 0


Query: 1   MVRPFAAKGKKRKKTERYDRDDDGEESTASAKKAMVENEPDEQPENEAAEETFHELEGIP 60
           MVRPFAAKGKKRKK+E+YDRD+D EEST+ +KK M+ENEPDE+P    A+E FHELEGIP
Sbjct: 33  MVRPFAAKGKKRKKSEKYDRDEDAEESTSPSKKVMLENEPDEEP----AKEDFHELEGIP 92

Query: 61  IVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSNFLRRNNRNPGDYRPDILH 120
           I PKDPK+DS AGVIF+LERASLEVAKVGKNYQLLNSDDHSN+LRRNNRNPGDYRPDILH
Sbjct: 93  IAPKDPKNDSNAGVIFILERASLEVAKVGKNYQLLNSDDHSNYLRRNNRNPGDYRPDILH 152

Query: 121 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 180
           QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA
Sbjct: 153 QALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQKRFYGVMLQLLQKLSITA 212

Query: 181 AGKREKLFRVIKNPVTQYLPSNSRKMGFSHSSDKLVKVRTYLDAVEDDVDLVFVVGAMAH 240
           AGKREKLFRVIKNPVTQYLP+N RKMGFSHSSDKLVKVR YLDAV+DDVDLVFVVGAMAH
Sbjct: 213 AGKREKLFRVIKNPVTQYLPANCRKMGFSHSSDKLVKVRNYLDAVKDDVDLVFVVGAMAH 272

Query: 241 GKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNVG 279
           GKIET YTDDLLAISEYPLSASCCIADICKDLA+KWNVG
Sbjct: 273 GKIETDYTDDLLAISEYPLSASCCIADICKDLAEKWNVG 307

[Close]

BLAST of Cp4.1LG03g08620 vs. TAIR 10
Match: AT3G57000.1 (nucleolar essential protein-related )

HSP 1 Score: 307.4 bits (786), Expect = 1.2e-83
Identity = 168/298 (56.38%), Postives = 211/298 (70.81%), Query Frame = 0


Query: 1   MVRPFAAKGKKRK-KTERYDRDDD-------------GEESTASAKKAMVENEPDEQPEN 60
           MVRP+  K  KRK + ERYD+++D               ES+  AKK       ++  E 
Sbjct: 1   MVRPYGIKVNKRKEREERYDKEEDEVEEQPKFEQKQKARESSKKAKKESTSRAEEDNDEE 60

Query: 61  EAAEETFHELE----GIPIVPKDPKSDSKAGVIFVLERASLEVAKVGKNYQLLNSDDHSN 120
           E   E     E    GIPIV   P  + K+G++FVLE+ASLEVAKVGK YQLLNSDDH+N
Sbjct: 61  EVTVEATAAAEDIVGGIPIVLNAPNKE-KSGIVFVLEKASLEVAKVGKTYQLLNSDDHAN 120

Query: 121 FLRRNNRNPGDYRPDILHQALLAIFDSRIAKAGRLKVVYVKTEKGLLIEIKPYVRLPRTQ 180
           FL++NNRNP DYRPDI HQALL I DS + KAGRLK VYV+TEKG+L E+KP+VR+PRT 
Sbjct: 121 FLKKNNRNPADYRPDITHQALLMILDSPVNKAGRLKAVYVRTEKGVLFEVKPHVRIPRTF 180

Query: 181 KRFYGVMLQLLQKLSITAAGKREKLFRVIKNPVTQ-YLPSNSRKMGFSHSSDKLVKVRTY 240
           KRF G+MLQLLQKLSITA   REKL R +KNP+ + +LP NS ++GFSHSS+KLV ++ +
Sbjct: 181 KRFAGIMLQLLQKLSITAVNSREKLLRCVKNPIEEHHLPVNSHRIGFSHSSEKLVNMQKH 240

Query: 241 LDAV-EDDVDLVFVVGAMAHGKIETGYTDDLLAISEYPLSASCCIADICKDLADKWNV 279
           L  V +DD D VFVVGAMAHGKI+  Y D+ +++SEYPLSA+ CI+ IC+ LA  WN+
Sbjct: 241 LATVCDDDRDTVFVVGAMAHGKIDCNYIDEFVSVSEYPLSAAYCISRICEALATNWNI 297

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g08620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O35130	9.6e-46	47.17	Ribosomal RNA small subunit methyltransferase NEP1 OS=Mus musculus OX=10090 GN=E...	[more]
Q92979	1.6e-45	43.98	Ribosomal RNA small subunit methyltransferase NEP1 OS=Homo sapiens OX=9606 GN=EM...	[more]
Q9XX15	1.1e-44	45.79	Ribosomal RNA small subunit methyltransferase nep-1 OS=Caenorhabditis elegans OX...	[more]
Q06287	3.1e-44	43.29	Ribosomal RNA small subunit methyltransferase NEP1 OS=Saccharomyces cerevisiae (...	[more]
Q10107	3.8e-42	42.15	Ribosomal RNA small subunit methyltransferase mra1 OS=Schizosaccharomyces pombe ...	[more]

BLAST of Cp4.1LG03g08620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527836.1	3.13e-194	100.00	ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita pepo subsp. pepo]	[more]
KAG7017643.1	1.81e-193	99.64	Ribosomal RNA small subunit methyltransferase NEP1, partial [Cucurbita argyrospe...	[more]
XP_022935622.1	1.05e-192	99.28	ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita moschata] >KAG658...	[more]
XP_022983847.1	1.49e-192	99.28	ribosomal RNA small subunit methyltransferase nep-1 [Cucurbita maxima]	[more]
XP_008443268.1	3.05e-175	90.32	PREDICTED: ribosomal RNA small subunit methyltransferase NEP1 [Cucumis melo] >KA...	[more]

BLAST of Cp4.1LG03g08620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F634	5.07e-193	99.28	ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita moschata OX=366...	[more]
A0A6J1J0G6	7.20e-193	99.28	ribosomal RNA small subunit methyltransferase nep-1 OS=Cucurbita maxima OX=3661 ...	[more]
A0A5D3DPQ6	1.47e-175	90.32	Ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo var. makuwa O...	[more]
A0A1S3B7L0	1.47e-175	90.32	ribosomal RNA small subunit methyltransferase NEP1 OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A0A0LFI3	4.73e-175	90.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G821550 PE=3 SV=1	[more]

BLAST of Cp4.1LG03g08620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G57000.1	1.2e-83	56.38	nucleolar essential protein-related	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	PFAM	PF03587	EMG1	coord: 76..271 e-value: 4.0E-65 score: 219.3
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	PANTHER	PTHR12636	NEP1/MRA1	coord: 10..278
IPR005304	Ribosomal biogenesis, methyltransferase, EMG1/NEP1	CDD	cd18088	Nep1-like	coord: 74..269 e-value: 2.75579E-95 score: 277.489
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 74..278 e-value: 5.7E-81 score: 272.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..51
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 12..40
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 67..278

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g08620.1	Cp4.1LG03g08620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070475	rRNA base methylation
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0032040	small-subunit processome
molecular_function	GO:0019843	rRNA binding
molecular_function	GO:0070037	rRNA (pseudouridine) methyltransferase activity