Cp4.1LG03g08140 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g08140
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Calcium-binding EF hand family protein
Location	Cp4.1LG03: 2970048 .. 2970608 (-)
RNA-Seq Expression	Cp4.1LG03g08140
Synteny	Cp4.1LG03g08140

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACAACCCACAACCCACCATCAATCTGCAGCTCATCCCGTCAACCACGACAACGACACCGCCGCTCGTTTTCATCGATCGGGCATCGATCTTCGCCGACACAATACACGACCTCATCTCTCTAGTCTTCTCATTCTTTCAATCGCCCCCAAAAGAGAAGGCTGCACCACAAAGCAGAGCCTCGCAATCAGAGGGCAAGGAAGATTGGTTAATGAGCAGAGAAGAAGTGAAATCTGTGATGGAAAAATTAGAGCTTTATTGCAACGAAGAAGAGGATGATGAAATTGATGAATGTTTTGGAAGGGATGAAGTAAGAGGCATGTTTGAAGAGAATGAACCGAGCTTGGAAGAGTTGAAGCAAACGTTCAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCACAGGAATTGCACACAGTTCTGGGCCTGCTTGCCTCTAATAATACCATATTCATCCATGACTGCAAATCAATGATAGCCAGATTTGATCATAACAATGATCAAAAAATTGATTTCAATGAGTTTGTAAAGTTTATGGAGGTTGCCTTGTCCTAA

mRNA sequence

ATGCACAACCCACAACCCACCATCAATCTGCAGCTCATCCCGTCAACCACGACAACGACACCGCCGCTCGTTTTCATCGATCGGGCATCGATCTTCGCCGACACAATACACGACCTCATCTCTCTAGTCTTCTCATTCTTTCAATCGCCCCCAAAAGAGAAGGCTGCACCACAAAGCAGAGCCTCGCAATCAGAGGGCAAGGAAGATTGGTTAATGAGCAGAGAAGAAGTGAAATCTGTGATGGAAAAATTAGAGCTTTATTGCAACGAAGAAGAGGATGATGAAATTGATGAATGTTTTGGAAGGGATGAAGTAAGAGGCATGTTTGAAGAGAATGAACCGAGCTTGGAAGAGTTGAAGCAAACGTTCAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCACAGGAATTGCACACAGTTCTGGGCCTGCTTGCCTCTAATAATACCATATTCATCCATGACTGCAAATCAATGATAGCCAGATTTGATCATAACAATGATCAAAAAATTGATTTCAATGAGTTTGTAAAGTTTATGGAGGTTGCCTTGTCCTAA

Coding sequence (CDS)

ATGCACAACCCACAACCCACCATCAATCTGCAGCTCATCCCGTCAACCACGACAACGACACCGCCGCTCGTTTTCATCGATCGGGCATCGATCTTCGCCGACACAATACACGACCTCATCTCTCTAGTCTTCTCATTCTTTCAATCGCCCCCAAAAGAGAAGGCTGCACCACAAAGCAGAGCCTCGCAATCAGAGGGCAAGGAAGATTGGTTAATGAGCAGAGAAGAAGTGAAATCTGTGATGGAAAAATTAGAGCTTTATTGCAACGAAGAAGAGGATGATGAAATTGATGAATGTTTTGGAAGGGATGAAGTAAGAGGCATGTTTGAAGAGAATGAACCGAGCTTGGAAGAGTTGAAGCAAACGTTCAATGTGTTTGATAGAAACAGAGATGGGTTCATTGATGCACAGGAATTGCACACAGTTCTGGGCCTGCTTGCCTCTAATAATACCATATTCATCCATGACTGCAAATCAATGATAGCCAGATTTGATCATAACAATGATCAAAAAATTGATTTCAATGAGTTTGTAAAGTTTATGGAGGTTGCCTTGTCCTAA

Protein sequence

MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSRASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVALS

Homology

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.2e-22
Identity = 59/117 (50.43%), Postives = 75/117 (64.10%), Query Frame = 0


Query: 68  EDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFD 127
           +D  +SREE + VM  L L+ N   DD++ E +   EV  +FEE E SLEE+KQ F+VFD
Sbjct: 79  DDIDISREEAEMVMRSLGLFYN---DDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 138

Query: 128 RNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVA 185
            N+DGFIDA EL  VL +L      ++ +C  MI   D N D KIDFNEFVKFME +
Sbjct: 139 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 9.7e-18
Identity = 51/111 (45.95%), Postives = 71/111 (63.96%), Query Frame = 0


Query: 72  MSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFDRNRD 131
           + RE+V  VM+ L L   ++E++ + + +   EV  +FEE EPSLEE+KQ F+VFD NRD
Sbjct: 91  LCREDVGMVMKSLGL-STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRD 150

Query: 132 GFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFME 183
           GFID  +L  VL +L       + +C+ MI  FD + D +IDF  FVKFME
Sbjct: 151 GFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.7e-10
Identity = 50/168 (29.76%), Postives = 84/168 (50.00%), Query Frame = 0


Query: 23  LVFIDRASIFADTIHDLIS---LVFSFFQSPPKEKAAPQSRASQSEGKEDWLMSREEVKS 82
           ++  D +S F    H   S   ++F+  ++  +    P  +      + D   S EEVK+
Sbjct: 26  MIIQDFSSSFPFRFHLFFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKA 85

Query: 83  VMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL-KQTFNVFDRNRDGFIDAQE 142
           ++         ++ + + EC   +    + E+NE   +E+ K+ F +FD N+DGFID  E
Sbjct: 86  II---------DDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENE 145

Query: 143 LHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVALS 187
           L  VL LL  +    + +C+ M+  +D N D KIDF EFVK +E + S
Sbjct: 146 LKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 3.7e-09
Identity = 54/207 (26.09%), Postives = 91/207 (43.96%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 60
           ++N   +  L L+P    T  PL + D+      T+H       S     P   +AP  R
Sbjct: 7   LYNILNSFLLSLVPKKLRTLFPLSWFDK------TLHKNSPPSPSTMLPSPSSSSAPTKR 66

Query: 61  ASQSEGKE---------DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRD 120
              SE K          D  +++EE+   +E L +Y  +++        D   D C   D
Sbjct: 67  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 126

Query: 121 EVRGMF--------EENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIH 180
           E   ++         + E   E++K  FNVFD++ DGFI  +EL +V+  L       + 
Sbjct: 127 EFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 186

Query: 181 DCKSMIARFDHNNDQKIDFNEFVKFME 183
            CK MI + D + D ++++ EF++ M+
Sbjct: 187 GCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.3e-09
Identity = 38/125 (30.40%), Postives = 67/125 (53.60%), Query Frame = 0


Query: 69  DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRDEVRGMFE---ENEPSLE 128
           D  ++++E+   +E L +Y  +++        D   D     +E  G+++   E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 129 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEF 183
           ++++ FNVFD+NRDGFI  +EL +VL  L       + DCK MI++ D + D  ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

[Close]

BLAST of Cp4.1LG03g08140 vs. NCBI nr
Match: XP_023528818.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370 bits (951), Expect = 3.49e-129
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR
Sbjct: 11  MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 70

Query: 61  ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 71  ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 130

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF
Sbjct: 131 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF 190

Query: 181 MEVALS 186
           MEVALS
Sbjct: 191 MEVALS 196

[Close]

BLAST of Cp4.1LG03g08140 vs. NCBI nr
Match: KAG7017701.1 (putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362 bits (929), Expect = 5.54e-126
Identity = 181/186 (97.31%), Postives = 183/186 (98.39%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDL+SL FSFFQSPPK+KAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSE KEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFD NNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 186
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Cp4.1LG03g08140 vs. NCBI nr
Match: XP_022934595.1 (probable calcium-binding protein CML30 [Cucurbita moschata])

HSP 1 Score: 359 bits (922), Expect = 6.47e-125
Identity = 180/186 (96.77%), Postives = 182/186 (97.85%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFA TIHDL+SL FSFFQSPPK+KAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFAGTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSE KEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFD NNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 186
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Cp4.1LG03g08140 vs. NCBI nr
Match: KAG6580956.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 359 bits (922), Expect = 6.47e-125
Identity = 180/186 (96.77%), Postives = 182/186 (97.85%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDL+SL FSFFQSPPK+KAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSE K DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKGDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFD NNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 186
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Cp4.1LG03g08140 vs. NCBI nr
Match: XP_022983385.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 346 bits (888), Expect = 1.02e-119
Identity = 175/187 (93.58%), Postives = 179/187 (95.72%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPP-LVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQS 60
           MHNPQPTINLQLIPSTTT  PP LVFIDRASIFADTIHDL+SL FSFFQSPPKEKAAPQS
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKEKAAPQS 60

Query: 61  RASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL 120
           RASQSEGKEDWLMSREEVKSVMEKLELYCNEE DDEIDECFGR+EVRGMFEENEPSLEEL
Sbjct: 61  RASQSEGKEDWLMSREEVKSVMEKLELYCNEEGDDEIDECFGREEVRGMFEENEPSLEEL 120

Query: 121 KQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVK 180
           KQTFNVFDRNRDGFIDA ELHTVLGLL SNNTIFIHDCKSMIARFD NND+KIDFNEFVK
Sbjct: 121 KQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVK 180

Query: 181 FMEVALS 186
           FMEV+LS
Sbjct: 181 FMEVSLS 187

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 359 bits (922), Expect = 3.13e-125
Identity = 180/186 (96.77%), Postives = 182/186 (97.85%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 60
           MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFA TIHDL+SL FSFFQSPPK+KAAPQSR
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFAGTIHDLVSLAFSFFQSPPKDKAAPQSR 60

Query: 61  ASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120
           ASQSE KEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK
Sbjct: 61  ASQSESKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELK 120

Query: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKF 180
           QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFD NNDQKIDFNEFVKF
Sbjct: 121 QTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKIDFNEFVKF 180

Query: 181 MEVALS 186
           MEVALS
Sbjct: 181 MEVALS 186

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 346 bits (888), Expect = 4.96e-120
Identity = 175/187 (93.58%), Postives = 179/187 (95.72%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPP-LVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQS 60
           MHNPQPTINLQLIPSTTT  PP LVFIDRASIFADTIHDL+SL FSFFQSPPKEKAAPQS
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASIFADTIHDLVSLAFSFFQSPPKEKAAPQS 60

Query: 61  RASQSEGKEDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL 120
           RASQSEGKEDWLMSREEVKSVMEKLELYCNEE DDEIDECFGR+EVRGMFEENEPSLEEL
Sbjct: 61  RASQSEGKEDWLMSREEVKSVMEKLELYCNEEGDDEIDECFGREEVRGMFEENEPSLEEL 120

Query: 121 KQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVK 180
           KQTFNVFDRNRDGFIDA ELHTVLGLL SNNTIFIHDCKSMIARFD NND+KIDFNEFVK
Sbjct: 121 KQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDEKIDFNEFVK 180

Query: 181 FMEVALS 186
           FMEV+LS
Sbjct: 181 FMEVSLS 187

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 195 bits (495), Expect = 3.09e-60
Identity = 109/190 (57.37%), Postives = 135/190 (71.05%), Query Frame = 0


Query: 6   PTINLQLIPSTTTTTPPLVFIDRASI------FADTIHDLISLVFSFFQSPPKEKAAPQS 65
           P +NL+L P+       L+ +DR SI      F D IHDLIS  +S F SPPKEK +PQ 
Sbjct: 6   PIVNLKLAPT-------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKVSPQG 65

Query: 66  RASQSEGKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECF-GRDEVRGMFEENEPSL 125
            + +SE  +  +  +S+EEVK VMEKLE +C+   D+EI+E + G +E+  +F E+EPSL
Sbjct: 66  NSLESEATQHKNSSISKEEVKFVMEKLEFFCS---DEEIEESWLGGEEIGAIFYESEPSL 125

Query: 126 EELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNE 185
           EELKQTFNVFD+NRDG IDA EL +VLGLL  N  +FIHDC  MIARFDHN D KIDFNE
Sbjct: 126 EELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNE 185

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 193 bits (490), Expect = 1.77e-59
Identity = 109/190 (57.37%), Postives = 135/190 (71.05%), Query Frame = 0


Query: 6   PTINLQLIPSTTTTTPPLVFIDRASI------FADTIHDLISLVFSFFQSPPKEKAAPQS 65
           P +NL+L  +       L+ +DR SI      F D IHDLIS  +S F SPPKEKA+PQ 
Sbjct: 6   PVVNLKLALT-------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQG 65

Query: 66  RASQSEGKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECF-GRDEVRGMFEENEPSL 125
            + +SE  +  +  +S+EEVK VMEKLE +C+   D+EI+E + G +E+  +F E+EPSL
Sbjct: 66  NSLESEATQHKNSSISKEEVKFVMEKLEFFCS---DEEIEESWLGGEEIGAIFYESEPSL 125

Query: 126 EELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNE 185
           EELKQTFNVFD+NRDG IDA EL +VLGLL  N  +FIHDC  MIARFDHN D KIDFNE
Sbjct: 126 EELKQTFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNE 185

[Close]

BLAST of Cp4.1LG03g08140 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 178 bits (452), Expect = 2.24e-53
Identity = 113/196 (57.65%), Postives = 128/196 (65.31%), Query Frame = 0


Query: 9   NLQLIPSTTTTTPPLVFIDRASI------FADTIHDLI-SLVFSFFQSPPKEKAAPQSRA 68
           ++QL PS      P VF+DR SI      FAD I +L+ SL +   Q   KEKA  Q   
Sbjct: 21  SVQLDPS------PFVFVDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQCNC 80

Query: 69  SQSEGKEDWL--------MSREEVKSVMEKLELYCNEEEDDEI-DECFGRDE--VRGMFE 128
           S   G +  +        MSREEV+ VMEKLEL+  EE D    D+  G  +  +RGMFE
Sbjct: 81  SSKSGDQIIITTQQNCSSMSREEVRFVMEKLELFWREERDGGGGDKGIGESDEMIRGMFE 140

Query: 129 ENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQ 186
           ENEPSLEELKQTFNVFDRNRDGFID  EL  VL LL SN  IFIHDCK+MIARFD NND 
Sbjct: 141 ENEPSLEELKQTFNVFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDG 200

[Close]

BLAST of Cp4.1LG03g08140 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 107.1 bits (266), Expect = 1.6e-23
Identity = 59/117 (50.43%), Postives = 75/117 (64.10%), Query Frame = 0


Query: 68  EDWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFD 127
           +D  +SREE + VM  L L+ N   DD++ E +   EV  +FEE E SLEE+KQ F+VFD
Sbjct: 79  DDIDISREEAEMVMRSLGLFYN---DDQLQEQYSAKEVSSLFEEKEASLEEVKQAFDVFD 138

Query: 128 RNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVA 185
            N+DGFIDA EL  VL +L      ++ +C  MI   D N D KIDFNEFVKFME +
Sbjct: 139 ENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKFMETS 192

[Close]

BLAST of Cp4.1LG03g08140 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 91.7 bits (226), Expect = 6.9e-19
Identity = 51/111 (45.95%), Postives = 71/111 (63.96%), Query Frame = 0


Query: 72  MSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEELKQTFNVFDRNRD 131
           + RE+V  VM+ L L   ++E++ + + +   EV  +FEE EPSLEE+KQ F+VFD NRD
Sbjct: 91  LCREDVGMVMKSLGL-STDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDENRD 150

Query: 132 GFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFME 183
           GFID  +L  VL +L       + +C+ MI  FD + D +IDF  FVKFME
Sbjct: 151 GFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFVKFME 200

[Close]

BLAST of Cp4.1LG03g08140 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 65.9 bits (159), Expect = 4.0e-11
Identity = 50/168 (29.76%), Postives = 84/168 (50.00%), Query Frame = 0


Query: 23  LVFIDRASIFADTIHDLIS---LVFSFFQSPPKEKAAPQSRASQSEGKEDWLMSREEVKS 82
           ++  D +S F    H   S   ++F+  ++  +    P  +      + D   S EEVK+
Sbjct: 26  MIIQDFSSSFPFRFHLFFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKA 85

Query: 83  VMEKLELYCNEEEDDEIDECFGRDEVRGMFEENEPSLEEL-KQTFNVFDRNRDGFIDAQE 142
           ++         ++ + + EC   +    + E+NE   +E+ K+ F +FD N+DGFID  E
Sbjct: 86  II---------DDSEALYECLIEEGEEYLLEKNEMMGKEIVKEAFRLFDENQDGFIDENE 145

Query: 143 LHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEFVKFMEVALS 187
           L  VL LL  +    + +C+ M+  +D N D KIDF EFVK +E + S
Sbjct: 146 LKHVLSLLGYDECTKM-ECRKMVKVYDENRDGKIDFYEFVKLIEKSFS 183

[Close]

BLAST of Cp4.1LG03g08140 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 63.2 bits (152), Expect = 2.6e-10
Identity = 54/207 (26.09%), Postives = 91/207 (43.96%), Query Frame = 0


Query: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASIFADTIHDLISLVFSFFQSPPKEKAAPQSR 60
           ++N   +  L L+P    T  PL + D+      T+H       S     P   +AP  R
Sbjct: 7   LYNILNSFLLSLVPKKLRTLFPLSWFDK------TLHKNSPPSPSTMLPSPSSSSAPTKR 66

Query: 61  ASQSEGKE---------DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRD 120
              SE K          D  +++EE+   +E L +Y  +++        D   D C   D
Sbjct: 67  IDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDID 126

Query: 121 EVRGMF--------EENEPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIH 180
           E   ++         + E   E++K  FNVFD++ DGFI  +EL +V+  L       + 
Sbjct: 127 EFESLYSSIVDEHHNDGETEEEDMKDAFNVFDQDGDGFITVEELKSVMASLGLKQGKTLD 186

Query: 181 DCKSMIARFDHNNDQKIDFNEFVKFME 183
            CK MI + D + D ++++ EF++ M+
Sbjct: 187 GCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]

BLAST of Cp4.1LG03g08140 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 62.4 bits (150), Expect = 4.5e-10
Identity = 38/125 (30.40%), Postives = 67/125 (53.60%), Query Frame = 0


Query: 69  DWLMSREEVKSVMEKLELYCNEEE--------DDEIDECFGRDEVRGMFE---ENEPSLE 128
           D  ++++E+   +E L +Y  +++        D   D     +E  G+++   E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 129 ELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDHNNDQKIDFNEF 183
           ++++ FNVFD+NRDGFI  +EL +VL  L       + DCK MI++ D + D  ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g08140 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	2.2e-22	50.43	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	9.7e-18	45.95	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9SN89	5.7e-10	29.76	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]
O22845	3.7e-09	26.09	Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2	[more]
Q9SRR7	6.3e-09	30.40	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]

BLAST of Cp4.1LG03g08140 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528818.1	3.49e-129	100.00	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
KAG7017701.1	5.54e-126	97.31	putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022934595.1	6.47e-125	96.77	probable calcium-binding protein CML30 [Cucurbita moschata]	[more]
KAG6580956.1	6.47e-125	96.77	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022983385.1	1.02e-119	93.58	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG03g08140 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F854	3.13e-125	96.77	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1J5Q6	4.96e-120	93.58	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1KQS0	3.09e-60	57.37	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]
A0A6J1EIK8	1.77e-59	57.37	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A0A0LHQ5	2.24e-53	57.65	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]

BLAST of Cp4.1LG03g08140 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	1.6e-23	50.43	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	6.9e-19	45.95	Calcium-binding EF-hand family protein	[more]
AT3G47480.1	4.0e-11	29.76	Calcium-binding EF-hand family protein	[more]
AT2G43290.1	2.6e-10	26.09	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	4.5e-10	30.40	ARF-GAP domain 11	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 156..184 e-value: 0.03 score: 23.5 coord: 118..146 e-value: 3.1E-6 score: 36.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 117..182 e-value: 1.7E-9 score: 38.0
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 152..186 score: 9.882769
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 114..149 score: 15.015522
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 118..181 e-value: 2.34227E-13 score: 59.8689
None	No IPR available	GENE3D	1.10.238.10		coord: 100..182 e-value: 4.6E-18 score: 67.3
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 59..184
None	No IPR available	PANTHER	PTHR10891:SF805	CALCIUM-BINDING PROTEIN CML45-RELATED	coord: 59..184
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 165..177
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 127..139
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 69..181

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g08140.1	Cp4.1LG03g08140.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding