Cp4.1LG03g08100 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g08100 vs. ExPASy Swiss-Prot
Match: Q43847 (Granule-bound starch synthase 2, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 GN=SS2 PE=1 SV=3)

HSP 1 Score: 946.4 bits (2445), Expect = 2.1e-274
Identity = 475/734 (64.71%), Postives = 564/734 (76.84%), Query Frame = 0

Query: 88  RARAATSDLNSGQRSDDPDQAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKGTSLDS 147
           R +A   +      +D+ + A+Q TIEKSKKVLAMQQ LL+QIAER+K+VSSIK +  ++
Sbjct: 43  RVKATGENSGEAASADESNDALQVTIEKSKKVLAMQQDLLQQIAERRKVVSSIKSSLANA 102

Query: 148 KEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNGSSLPVSNDNLDVRKQLDAVTTFSSRPS 207
           K   +          IPD   ++   TV S   + +   + N       D V +      
Sbjct: 103 KGTYDGGSGSLSDVDIPDV-DKDYNVTVPSTAATPITDVDKNTPPAISQDFVESKREIKR 162

Query: 208 DKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYSDVLPNRKDQLKGSSKKVLPD--SKHQL 267
           D  DE+     +  I+++       SS +        N   +   SS++ L D  S+  L
Sbjct: 163 DLADERAPPLSRSSITASSQISSTVSSKRTL------NVPPETPKSSQETLLDVNSRKSL 222

Query: 268 KGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSEPTLKVDSDAAESESVDVKPSPLAGV 327
                KK   ++P+ L  +SS S V E++N      + + + +  +  ++D KP PLAG 
Sbjct: 223 VDVPGKKIQSYMPS-LRKESSASHV-EQRNENLEGSSAEANEETEDPVNIDEKPPPLAGT 282

Query: 328 NVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGVRKR 387
           NVMN+I+VA+ECAPWSKTGGLGDVAG+LPKALARRGHRVMV+ PRY NY   QD+GVRK 
Sbjct: 283 NVMNIILVASECAPWSKTGGLGDVAGALPKALARRGHRVMVVAPRYDNYPEPQDSGVRKI 342

Query: 388 YKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYGGDHQDILKRMTFFCKAAVEVP 447
           YKVDGQD+EV +FQ ++DGVDFVFID++MF H   NIYGG+  DILKRM  FCKAA+EVP
Sbjct: 343 YKVDGQDVEVTYFQAFIDGVDFVFIDSHMFRHIGNNIYGGNRVDILKRMVLFCKAAIEVP 402

Query: 448 WHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHNIAHQGRGP 507
           WHVPCGGVCYGDGNLVF+ANDWHTA+LPVYLKAYYRDNG+M +TRS+LVIHNIAHQGRGP
Sbjct: 403 WHVPCGGVCYGDGNLVFIANDWHTALLPVYLKAYYRDNGIMNYTRSVLVIHNIAHQGRGP 462

Query: 508 VDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGLKAADRVVTVSHGYAWELKTIEGGWG 567
           ++DF  VDLP HYMD FK+YDPVGGEHFNIFAAGLK ADRVVTVSHGY+WELKT +GGWG
Sbjct: 463 LEDFSYVDLPPHYMDPFKLYDPVGGEHFNIFAAGLKTADRVVTVSHGYSWELKTSQGGWG 522

Query: 568 LHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTNYSIETLQTGKPQCKAALQKEL 627
           LH IINE DWK+ GIVNGID+KEW+P+ D+HL SDGY NYS++TLQTGKPQCKAALQKEL
Sbjct: 523 LHQIINENDWKLQGIVNGIDTKEWNPELDVHLQSDGYMNYSLDTLQTGKPQCKAALQKEL 582

Query: 628 GLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQLVLLGTGRQDLEQMLRDFENQ 687
           GLPVR+DVPLIGFIGRLD QKGVD+IAEA+PWM+GQDVQLV+LGTGR+DLEQMLR FE Q
Sbjct: 583 GLPVRDDVPLIGFIGRLDPQKGVDLIAEAVPWMMGQDVQLVMLGTGRRDLEQMLRQFECQ 642

Query: 688 HRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDT 747
           H DK+RGWVGFSVK +HRITAG DILLMPSRFEPCGLNQLYAM YGTIPVVH+VGGLRDT
Sbjct: 643 HNDKIRGWVGFSVKTSHRITAGADILLMPSRFEPCGLNQLYAMKYGTIPVVHAVGGLRDT 702

Query: 748 VIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAA 807
           V PFDPFNESG GWTF  AEA++LIHALGNCLLTYR+YKKSWEG+Q R MTQDLSWD+AA
Sbjct: 703 VQPFDPFNESGLGWTFSRAEASQLIHALGNCLLTYREYKKSWEGIQTRCMTQDLSWDNAA 762

Query: 808 ERYEEVLVAAKYQW 820
           + YEEVL+AAKYQW
Sbjct: 763 QNYEEVLIAAKYQW 767

BLAST of Cp4.1LG03g08100 vs. ExPASy Swiss-Prot
Match: Q9MAC8 (Starch synthase 2, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=SS2 PE=1 SV=1)

HSP 1 Score: 941.4 bits (2432), Expect = 6.7e-273
Identity = 477/732 (65.16%), Postives = 560/732 (76.50%), Query Frame = 0

Query: 98  SGQRSDDPDQAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQM 157
           SG   D+P+ A+QATI+KSKKVLAMQ+ LL QIAER+KLVSSIK ++ D  + + S++Q 
Sbjct: 63  SGSDDDEPEDALQATIDKSKKVLAMQRNLLHQIAERRKLVSSIKESTPDLDDAKASSKQE 122

Query: 158 DGSFPIPDFPSRNDEGTVDSLNGSSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEK---Q 217
             S    +  +   E      NGS  P +     + K+ +A T   S  S KN ++    
Sbjct: 123 SASSVNANTDATKKEIMDGDANGSVSPSTYGKSSLSKEPEAKTFSPSTESLKNRKQSSAS 182

Query: 218 VVDLKKIISSAGPKDQL---KSSSKIKYSDVLPNRKDQLKGSSKKVLPDSKHQLKGSSKK 277
           V+    + S   P D     K  S +  S V P  K     +S +   D        SK 
Sbjct: 183 VISSSPVTSPQKPSDVATNGKPWSSVVASSVDPPYKPSSVMTSPEKTSDPVTSPGKPSKS 242

Query: 278 KS----SDFVPAFLASKSSYSAVKEEKNGQPSEPTLKVDSDAAESESVDVKPSPLAGVNV 337
           ++    SD +P++L      S +K EK  + +       S+  E    + KP PLAG NV
Sbjct: 243 RAGAFWSDPLPSYLTKAPQTSTMKTEKYVEKTPDV--ASSETNEPGKDEEKPPPLAGANV 302

Query: 338 MNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGVRKRYK 397
           MNVI+VAAECAP+SKTGGLGDVAG+LPK+LARRGHRVMV+VPRYA YA  +D GVRKRYK
Sbjct: 303 MNVILVAAECAPFSKTGGLGDVAGALPKSLARRGHRVMVVVPRYAEYAEAKDLGVRKRYK 362

Query: 398 VDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYGGDHQDILKRMTFFCKAAVEVPWH 457
           V GQD+EV +F  ++DGVDFVFID+  F H   NIYGG+  DILKRM  FCKAAVEVPW+
Sbjct: 363 VAGQDMEVMYFHAFIDGVDFVFIDSPEFRHLSNNIYGGNRLDILKRMVLFCKAAVEVPWY 422

Query: 458 VPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHNIAHQGRGPVD 517
           VPCGGVCYGDGNL F+ANDWHTA+LPVYLKAYYRD+G+M +TRS+LVIHNIAHQGRGPVD
Sbjct: 423 VPCGGVCYGDGNLAFIANDWHTALLPVYLKAYYRDHGIMKYTRSVLVIHNIAHQGRGPVD 482

Query: 518 DFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGLKAADRVVTVSHGYAWELKTIEGGWGLH 577
           DF  VDLP HY+D FK+YDPVGGEHFNIFAAGLKAADRV+TVSHGY+WE+KT+EGGWGLH
Sbjct: 483 DFSYVDLPSHYLDSFKLYDPVGGEHFNIFAAGLKAADRVLTVSHGYSWEVKTLEGGWGLH 542

Query: 578 GIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTNYSIETLQTGKPQCKAALQKELGL 637
            IINE DWK  GIVNGID++EW+P++D +L SD YTNYS+E L  GKPQCKAALQKELGL
Sbjct: 543 NIINENDWKFRGIVNGIDTQEWNPEFDTYLHSDDYTNYSLENLHIGKPQCKAALQKELGL 602

Query: 638 PVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQLVLLGTGRQDLEQMLRDFENQHR 697
           PVR DVPLIGFIGRLDHQKGVD+IAEA+PWM+ QDVQLV+LGTGR DLE++LR  E+Q+R
Sbjct: 603 PVRPDVPLIGFIGRLDHQKGVDLIAEAVPWMMSQDVQLVMLGTGRPDLEEVLRQMEHQYR 662

Query: 698 DKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDTVI 757
           DK RGWVGFSVK AHRITAG DILLMPSRFEPCGLNQLYAMNYGTIPVVH+VGGLRDTV 
Sbjct: 663 DKARGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLRDTVQ 722

Query: 758 PFDPFNESGFGWTFDSAEANKLIHALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAAER 817
            FDP++E+G GWTFDSAEA KLIHALGNCLLTYR+YK+SWEGLQRRGMTQDLSWD+AAE+
Sbjct: 723 QFDPYSETGLGWTFDSAEAGKLIHALGNCLLTYREYKESWEGLQRRGMTQDLSWDNAAEK 782

Query: 818 YEEVLVAAKYQW 820
           YEEVLVAAKY W
Sbjct: 783 YEEVLVAAKYHW 792

BLAST of Cp4.1LG03g08100 vs. ExPASy Swiss-Prot
Match: Q43093 (Granule-bound starch synthase 2, chloroplastic/amyloplastic OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 2.3e-265
Identity = 473/758 (62.40%), Postives = 561/758 (74.01%), Query Frame = 0

Query: 68  GVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVLAMQQQLL 127
           GVG L    V  N K       ++  +D N     DD    + ATIEKSKKVLA+Q++L+
Sbjct: 39  GVGRLNCGSVRLNHKQHVRAVGKSFGADENGDGSEDD---VVNATIEKSKKVLALQRELI 98

Query: 128 EQIAERKKLVSSIKGTSLDSKEEQN-SNEQMDGSFPIPDFPSRNDEGTVDSLNGSSLPVS 187
           +QIAERKKLVSSI   S+   E    S E  + S      P +       S +GS++   
Sbjct: 99  QQIAERKKLVSSIDSDSIPGLEGNGVSYESSEKSLSRDSNPQKG-----SSSSGSAVETK 158

Query: 188 N----DNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYSDV 247
                  L   K+ +     S   +   DE +    KK        D +K+SSK+ +++ 
Sbjct: 159 RWHCFQQLCRSKETETWAVSSVGINQGFDEIE----KK-------NDAVKASSKLHFNEQ 218

Query: 248 LPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSEPT 307
           + N+  +         PD+K                  ++S    S++K E     +EP+
Sbjct: 219 IKNKLYE--------RPDTKD-----------------ISSSIRTSSLKFENFEGANEPS 278

Query: 308 LK-VDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRG 367
            K V ++A   ES   KP PLAG NVMN+I+V+AECAPWSKTGGLGDVAGSLPKALARRG
Sbjct: 279 SKEVANEAENFESGGEKPPPLAGTNVMNIILVSAECAPWSKTGGLGDVAGSLPKALARRG 338

Query: 368 HRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQN 427
           HRVM++ P Y NYA   D GVRKRYKV GQD+EV +F TY+DGVD VFID+ +F + E N
Sbjct: 339 HRVMIVAPHYGNYAEAHDIGVRKRYKVAGQDMEVTYFHTYIDGVDIVFIDSPIFRNLESN 398

Query: 428 IYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYR 487
           IYGG+  DIL+RM  FCKAAVEVPWHVPCGG+CYGDGNLVF+ANDWHTA+LPVYLKAYYR
Sbjct: 399 IYGGNRLDILRRMVLFCKAAVEVPWHVPCGGICYGDGNLVFIANDWHTALLPVYLKAYYR 458

Query: 488 DNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGLK 547
           D+GLM +TRS+LVIHNIAHQGRGPV+DF  VDL  +Y+DLFKMYDPVGGEHFNIFAAGLK
Sbjct: 459 DHGLMNYTRSVLVIHNIAHQGRGPVEDFNTVDLSGNYLDLFKMYDPVGGEHFNIFAAGLK 518

Query: 548 AADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDG 607
            ADR+VTVSHGYAWELKT EGGWGLH IINE DWK  GIVNG+D+K+W+PQ+D +LTSDG
Sbjct: 519 TADRIVTVSHGYAWELKTSEGGWGLHNIINESDWKFRGIVNGVDTKDWNPQFDAYLTSDG 578

Query: 608 YTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQ 667
           YTNY+++TLQTGK QCKAALQ+ELGLPVREDVP+I FIGRLDHQKGVD+IAEAIPWM+  
Sbjct: 579 YTNYNLKTLQTGKRQCKAALQRELGLPVREDVPIISFIGRLDHQKGVDLIAEAIPWMMSH 638

Query: 668 DVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCG 727
           DVQLV+LGTGR DLEQML++FE QH DK+R WVGFSVKMAHRITAG DILLMPSRFEPCG
Sbjct: 639 DVQLVMLGTGRADLEQMLKEFEAQHCDKIRSWVGFSVKMAHRITAGSDILLMPSRFEPCG 698

Query: 728 LNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTYR 787
           LNQLYAM+YGT+PVVH VGGLRDTV PF+PF+ESG GWTFD AEANKL+ AL NCLLTY+
Sbjct: 699 LNQLYAMSYGTVPVVHGVGGLRDTVQPFNPFDESGVGWTFDRAEANKLMAALWNCLLTYK 752

Query: 788 QYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 820
            YKKSWEG+Q RGM+QDLSWD+AA++YEEVLVAAKYQW
Sbjct: 759 DYKKSWEGIQERGMSQDLSWDNAAQQYEEVLVAAKYQW 752

BLAST of Cp4.1LG03g08100 vs. ExPASy Swiss-Prot
Match: P0C586 (Soluble starch synthase 2-3, chloroplastic/amyloplastic OS=Oryza sativa subsp. indica OX=39946 GN=SSII-3 PE=1 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 1.1e-243
Identity = 389/515 (75.53%), Postives = 445/515 (86.41%), Query Frame = 0

Query: 306 DSDAAESE-SVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRV 365
           DSDA++SE   D    PLAG NVMNVI+VAAEC+PW KTGGLGDVAG+LPKALARRGHRV
Sbjct: 296 DSDASDSEIDQDDDSGPLAGENVMNVIVVAAECSPWCKTGGLGDVAGALPKALARRGHRV 355

Query: 366 MVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYG 425
           MV+VPRY +YA  QD G+RK YK  GQDLEV +F  ++DGVDFVFID  +F HR+ +IYG
Sbjct: 356 MVVVPRYGDYAEAQDVGIRKYYKAAGQDLEVKYFHAFIDGVDFVFIDAPLFRHRQDDIYG 415

Query: 426 GDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNG 485
           G+ Q+I+KRM  FCKAAVEVPWHVPCGGV YGDGNLVF+ANDWHTA+LPVYLKAYYRDNG
Sbjct: 416 GNRQEIMKRMILFCKAAVEVPWHVPCGGVPYGDGNLVFLANDWHTALLPVYLKAYYRDNG 475

Query: 486 LMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGLKAAD 545
           +M +TRS+LVIHNIA+QGRGPVD+F  ++LPEHY+D FK+YDPVGGEH NIF AGLK AD
Sbjct: 476 MMQYTRSVLVIHNIAYQGRGPVDEFPYMELPEHYLDHFKLYDPVGGEHANIFGAGLKMAD 535

Query: 546 RVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTN 605
           RVVTVS GY WELKT EGGWGLH II E DWKM GIVNGID +EW+P+ D+HL SDGY N
Sbjct: 536 RVVTVSPGYLWELKTTEGGWGLHDIIRENDWKMNGIVNGIDYREWNPEVDVHLQSDGYAN 595

Query: 606 YSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQ 665
           Y++ +L +GKP+CKAALQ+ELGL VR+DVPLIGFIGRLD QKGVDII +A+PW+ GQDVQ
Sbjct: 596 YTVASLDSGKPRCKAALQRELGLEVRDDVPLIGFIGRLDGQKGVDIIGDAMPWIAGQDVQ 655

Query: 666 LVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQ 725
           LVLLG+GR+DLE ML+ FE QH  KVRGWVGFSVKMAHRITAG D+L+MPSRFEPCGLNQ
Sbjct: 656 LVLLGSGRRDLEVMLQRFEAQHNSKVRGWVGFSVKMAHRITAGADVLVMPSRFEPCGLNQ 715

Query: 726 LYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTYRQYK 785
           LYAM YGT+PVVH+VGGLRDTV  FDPF ++G GWTFD AE +KLI ALG+CL TYR+YK
Sbjct: 716 LYAMAYGTVPVVHAVGGLRDTVSAFDPFEDTGLGWTFDRAEPHKLIEALGHCLETYRKYK 775

Query: 786 KSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 820
           +SW GLQ RGM+QDLSWDHAAE YEEVLV AKYQW
Sbjct: 776 ESWRGLQVRGMSQDLSWDHAAELYEEVLVKAKYQW 810

BLAST of Cp4.1LG03g08100 vs. ExPASy Swiss-Prot
Match: Q0DDE3 (Soluble starch synthase 2-3, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=SSII-3 PE=1 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 1.2e-242
Identity = 387/515 (75.15%), Postives = 444/515 (86.21%), Query Frame = 0

Query: 306 DSDAAESE-SVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRV 365
           DSDA++SE   D    PLAG NVMNVI+VAAEC+PW KTGGLGDVAG+LPKALARRGHRV
Sbjct: 296 DSDASDSEIDQDDDSGPLAGENVMNVIVVAAECSPWCKTGGLGDVAGALPKALARRGHRV 355

Query: 366 MVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYG 425
           MV+VPRY +YA  QD G+RK YK  GQDLEV +F  ++DGVDFVFID  +F HR+ +IYG
Sbjct: 356 MVVVPRYGDYAEAQDVGIRKYYKAAGQDLEVKYFHAFIDGVDFVFIDAPLFRHRQDDIYG 415

Query: 426 GDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNG 485
           G+ Q+I+KRM  FCKAAVEVPWHVPCGGV YGDGNLVF+ANDWHTA+LPVYLKAYYRDNG
Sbjct: 416 GNRQEIMKRMILFCKAAVEVPWHVPCGGVPYGDGNLVFLANDWHTALLPVYLKAYYRDNG 475

Query: 486 LMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGLKAAD 545
           +M +TRS+LVIHNIA+QGRGPVD+F  ++LPEHY+D FK+YDPVGGEH NIF AGLK AD
Sbjct: 476 MMQYTRSVLVIHNIAYQGRGPVDEFPYMELPEHYLDHFKLYDPVGGEHANIFGAGLKMAD 535

Query: 546 RVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTN 605
           RVVTVS GY WELKT EGGWGLH II E DWKM GIVNGID +EW+P+ D+HL SDGY N
Sbjct: 536 RVVTVSPGYLWELKTTEGGWGLHDIIRENDWKMNGIVNGIDYREWNPEVDVHLQSDGYAN 595

Query: 606 YSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQ 665
           Y++ +L + KP+CKAALQ+ELGL VR+DVPLIGFIGRLD QKGVDII +A+PW+ GQDVQ
Sbjct: 596 YTVASLDSSKPRCKAALQRELGLEVRDDVPLIGFIGRLDGQKGVDIIGDAMPWIAGQDVQ 655

Query: 666 LVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQ 725
           LVLLG+GR+DLE ML+ FE QH  KVRGWVGFSVKMAHRITAG D+L+MPSRFEPCGLNQ
Sbjct: 656 LVLLGSGRRDLEVMLQRFEAQHNSKVRGWVGFSVKMAHRITAGADVLVMPSRFEPCGLNQ 715

Query: 726 LYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTYRQYK 785
           LYAM YGT+PVVH+VGGLRDT+  FDPF ++G GWTFD AE +KLI ALG+CL TYR+YK
Sbjct: 716 LYAMAYGTVPVVHAVGGLRDTMSAFDPFEDTGLGWTFDRAEPHKLIEALGHCLETYRKYK 775

Query: 786 KSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 820
           +SW GLQ RGM+QDLSWDHAAE YEEVLV AKYQW
Sbjct: 776 ESWRGLQVRGMSQDLSWDHAAELYEEVLVKAKYQW 810

BLAST of Cp4.1LG03g08100 vs. NCBI nr
Match: XP_023526580.1 (granule-bound starch synthase 2, chloroplastic/amyloplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1647 bits (4265), Expect = 0.0
Identity = 819/819 (100.00%), Postives = 819/819 (100.00%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG
Sbjct: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Cp4.1LG03g08100 vs. NCBI nr
Match: KAG7017703.1 (Granule-bound starch synthase 2, chloroplastic/amyloplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1641 bits (4250), Expect = 0.0
Identity = 816/819 (99.63%), Postives = 817/819 (99.76%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTE SMIVLSNTYHRTVCSSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTETSMIVLSNTYHRTVCSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG
Sbjct: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAV TFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVPTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLP+RKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPDRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Cp4.1LG03g08100 vs. NCBI nr
Match: XP_022935687.1 (granule-bound starch synthase 2, chloroplastic/amyloplastic-like isoform X1 [Cucurbita moschata] >XP_022935688.1 granule-bound starch synthase 2, chloroplastic/amyloplastic-like isoform X1 [Cucurbita moschata] >XP_022935690.1 granule-bound starch synthase 2, chloroplastic/amyloplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1637 bits (4240), Expect = 0.0
Identity = 814/819 (99.39%), Postives = 815/819 (99.51%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTE SMIVLSNTYHRTVCSSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTETSMIVLSNTYHRTVCSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQ IQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQVIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAERKKLV SIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG
Sbjct: 121 AMQQQLLEQIAERKKLVLSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAV TFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVPTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLP+RKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPDRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Cp4.1LG03g08100 vs. NCBI nr
Match: XP_022983709.1 (granule-bound starch synthase 2, chloroplastic/amyloplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 1619 bits (4192), Expect = 0.0
Identity = 804/819 (98.17%), Postives = 812/819 (99.15%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMV +GSLPFTIEAYTE SMIVLSNTYHRTV SSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVSVGSLPFTIEAYTETSMIVLSNTYHRTVSSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGR+GKWPTPLRARAATSDLNSGQRSDDPD+AIQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRSGKWPTPLRARAATSDLNSGQRSDDPDEAIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAER+KLVSSIKGTSLDSKEEQNSNEQMDGSFPIP FPS NDEGTVDSLNG
Sbjct: 121 AMQQQLLEQIAERRKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPGFPSCNDEGTVDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAV TFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKS SKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVPTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSISKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLP+RKDQLKGSSKK+LPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPDRKDQLKGSSKKILPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDF+RVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFHRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKL+HALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLVHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Cp4.1LG03g08100 vs. NCBI nr
Match: XP_023526581.1 (granule-bound starch synthase 2, chloroplastic/amyloplastic isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1570 bits (4064), Expect = 0.0
Identity = 788/819 (96.21%), Postives = 788/819 (96.21%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAERKKLVSSIKGT                               VDSLNG
Sbjct: 121 AMQQQLLEQIAERKKLVSSIKGT-------------------------------VDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 788

BLAST of Cp4.1LG03g08100 vs. ExPASy TrEMBL
Match: A0A6J1F6A5 (Starch synthase, chloroplastic/amyloplastic OS=Cucurbita moschata OX=3662 GN=LOC111442471 PE=3 SV=1)

HSP 1 Score: 1637 bits (4240), Expect = 0.0
Identity = 814/819 (99.39%), Postives = 815/819 (99.51%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTE SMIVLSNTYHRTVCSSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTETSMIVLSNTYHRTVCSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQ IQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQVIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAERKKLV SIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG
Sbjct: 121 AMQQQLLEQIAERKKLVLSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAV TFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVPTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLP+RKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPDRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Cp4.1LG03g08100 vs. ExPASy TrEMBL
Match: A0A6J1J895 (Starch synthase, chloroplastic/amyloplastic OS=Cucurbita maxima OX=3661 GN=LOC111482246 PE=3 SV=1)

HSP 1 Score: 1619 bits (4192), Expect = 0.0
Identity = 804/819 (98.17%), Postives = 812/819 (99.15%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMV +GSLPFTIEAYTE SMIVLSNTYHRTV SSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVSVGSLPFTIEAYTETSMIVLSNTYHRTVSSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGR+GKWPTPLRARAATSDLNSGQRSDDPD+AIQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRSGKWPTPLRARAATSDLNSGQRSDDPDEAIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAER+KLVSSIKGTSLDSKEEQNSNEQMDGSFPIP FPS NDEGTVDSLNG
Sbjct: 121 AMQQQLLEQIAERRKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPGFPSCNDEGTVDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAV TFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKS SKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVPTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSISKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLP+RKDQLKGSSKK+LPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPDRKDQLKGSSKKILPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDF+RVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFHRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKL+HALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLVHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819

BLAST of Cp4.1LG03g08100 vs. ExPASy TrEMBL
Match: A0A6J1F649 (Starch synthase, chloroplastic/amyloplastic OS=Cucurbita moschata OX=3662 GN=LOC111442471 PE=3 SV=1)

HSP 1 Score: 1560 bits (4039), Expect = 0.0
Identity = 783/819 (95.60%), Postives = 784/819 (95.73%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTE SMIVLSNTYHRTVCSSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTETSMIVLSNTYHRTVCSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQ IQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQVIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAERKKLV SIKGT                               VDSLNG
Sbjct: 121 AMQQQLLEQIAERKKLVLSIKGT-------------------------------VDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAV TFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVPTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLP+RKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPDRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 788

BLAST of Cp4.1LG03g08100 vs. ExPASy TrEMBL
Match: A0A6J1J6P0 (Starch synthase, chloroplastic/amyloplastic OS=Cucurbita maxima OX=3661 GN=LOC111482246 PE=3 SV=1)

HSP 1 Score: 1547 bits (4006), Expect = 0.0
Identity = 775/819 (94.63%), Postives = 783/819 (95.60%), Query Frame = 0

Query: 1   MRASSGSPSISGPSLGFLLPLRREMVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQ 60
           MRASSGSPSISGPSLGFLLPLRREMV +GSLPFTIEAYTE SMIVLSNTYHRTV SSLKQ
Sbjct: 1   MRASSGSPSISGPSLGFLLPLRREMVSVGSLPFTIEAYTETSMIVLSNTYHRTVSSSLKQ 60

Query: 61  QNQRSAEGVGFLVDKMVGRNGKWPTPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVL 120
           QNQRSAEGVGFLVDKMVGR+GKWPTPLRARAATSDLNSGQRSDDPD+AIQATIEKSKKVL
Sbjct: 61  QNQRSAEGVGFLVDKMVGRSGKWPTPLRARAATSDLNSGQRSDDPDEAIQATIEKSKKVL 120

Query: 121 AMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNG 180
           AMQQQLLEQIAER+KLVSSIKGT                               VDSLNG
Sbjct: 121 AMQQQLLEQIAERRKLVSSIKGT-------------------------------VDSLNG 180

Query: 181 SSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYS 240
           SSLPVSNDNLDVRKQLDAV TFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKS SKIKYS
Sbjct: 181 SSLPVSNDNLDVRKQLDAVPTFSSRPSDKNDEKQVVDLKKIISSAGPKDQLKSISKIKYS 240

Query: 241 DVLPNRKDQLKGSSKKVLPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300
           DVLP+RKDQLKGSSKK+LPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE
Sbjct: 241 DVLPDRKDQLKGSSKKILPDSKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSE 300

Query: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360
           PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR
Sbjct: 301 PTLKVDSDAAESESVDVKPSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARR 360

Query: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420
           GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ
Sbjct: 361 GHRVMVIVPRYANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQ 420

Query: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480
           NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY
Sbjct: 421 NIYGGDHQDILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYY 480

Query: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540
           RDNGLMIFTRSILVIHNIAHQGRGPVDDF+RVDLPEHYMDLFKMYDPVGGEHFNIFAAGL
Sbjct: 481 RDNGLMIFTRSILVIHNIAHQGRGPVDDFHRVDLPEHYMDLFKMYDPVGGEHFNIFAAGL 540

Query: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600
           KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD
Sbjct: 541 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 600

Query: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660
           GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG
Sbjct: 601 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 660

Query: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720
           QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC
Sbjct: 661 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 720

Query: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTY 780
           GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKL+HALGNCLLTY
Sbjct: 721 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNESGFGWTFDSAEANKLVHALGNCLLTY 780

Query: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 819
           RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW
Sbjct: 781 RQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 788

BLAST of Cp4.1LG03g08100 vs. ExPASy TrEMBL
Match: A0A5D3DQS9 (Starch synthase, chloroplastic/amyloplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007900 PE=3 SV=1)

HSP 1 Score: 1360 bits (3519), Expect = 0.0
Identity = 680/799 (85.11%), Postives = 729/799 (91.24%), Query Frame = 0

Query: 25  MVFIGSLPFTIEAYTEASMIVLSNTYHRTVCSSLKQQNQRSAEGVGFLVDKMVGRNGKWP 84
           M  +GSLPFTIEA TE S+ VLS  YHR+  SSLK QNQR AEG+ FL DKM+ R GK P
Sbjct: 1   MASLGSLPFTIEAKTETSLFVLSKNYHRSF-SSLKPQNQRPAEGLSFLGDKMILRCGKLP 60

Query: 85  TPLRARAATSDLNSGQRSDDPDQAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKGTS 144
            P++ARAA SD +SGQRSDDPD+AIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIK T 
Sbjct: 61  KPMQARAAISD-SSGQRSDDPDEAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKST- 120

Query: 145 LDSKEEQNSNEQMDGSFPIPDFPSRNDEGTVDSLNGSSLPVSNDNLDVRKQLDAVTTFSS 204
           +DS E+Q+SNEQMDG FP PDFPSRNDE +VD LNGSSL VSN N+ VRKQLDAV + SS
Sbjct: 121 VDSTEKQDSNEQMDGLFPSPDFPSRNDEISVDVLNGSSLSVSNGNVAVRKQLDAVPSSSS 180

Query: 205 RPSDKNDEKQVVDLKKIISSAGPKDQLKSSSKIKYSDVLPNRKDQLKGSSKK----VLPD 264
           RPSDK+DEKQVVDLKK I SA PKDQLK SS+  YSD+L + KDQLKGSSK     +L D
Sbjct: 181 RPSDKDDEKQVVDLKKKIYSADPKDQLKGSSRTTYSDILADPKDQLKGSSKSKSSDILQD 240

Query: 265 SKHQLKGSSKKKSSDFVPAFLASKSSYSAVKEEKNGQPSEPTLKVDSDAAESESVDVKPS 324
           S+ QLKGSSKK SSDFVPAFL+SKSS+S+VKE K+ QPSEPTLK DSD  E+  VDVKP 
Sbjct: 241 SEDQLKGSSKKNSSDFVPAFLSSKSSHSSVKEGKDRQPSEPTLKDDSDTVETAGVDVKPP 300

Query: 325 PLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDT 384
           PLAGVNVMN++IVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDT
Sbjct: 301 PLAGVNVMNIVIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDT 360

Query: 385 GVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYGGDHQDILKRMTFFCKA 444
           GVRKRYKV GQD EV++FQTYVDGVDFVFIDN+MFH+REQNIYGGD  +ILKRM  FCKA
Sbjct: 361 GVRKRYKVHGQDYEVSYFQTYVDGVDFVFIDNSMFHNREQNIYGGDRLEILKRMVLFCKA 420

Query: 445 AVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHNIAH 504
           A+EVPW+VPCGGVCYGDGNLVFVANDWHTA+LPVYLKAYYRDNG+M FTRSILVIHNIAH
Sbjct: 421 AIEVPWYVPCGGVCYGDGNLVFVANDWHTALLPVYLKAYYRDNGIMKFTRSILVIHNIAH 480

Query: 505 QGRGPVDDFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGLKAADRVVTVSHGYAWELKTI 564
           QGRGPVDDFY VDLPEHYMD FK+YDP+GGEHFNIFAAGLK ADRVVTVSHGYAWELKT 
Sbjct: 481 QGRGPVDDFYHVDLPEHYMDHFKLYDPIGGEHFNIFAAGLKTADRVVTVSHGYAWELKTS 540

Query: 565 EGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTNYSIETLQTGKPQCKAA 624
           EGGWGLHGIINEIDWKM GIVNGIDSKEWSP+YD+HLTSDGYTNYS+ETLQTGKPQCKAA
Sbjct: 541 EGGWGLHGIINEIDWKMMGIVNGIDSKEWSPEYDVHLTSDGYTNYSLETLQTGKPQCKAA 600

Query: 625 LQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQLVLLGTGRQDLEQMLR 684
           LQKELGLP+REDVPLIGFIGRLD QKGVDIIAEAIPWMVGQDVQLV+LGTGRQDLEQMLR
Sbjct: 601 LQKELGLPIREDVPLIGFIGRLDQQKGVDIIAEAIPWMVGQDVQLVMLGTGRQDLEQMLR 660

Query: 685 DFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVG 744
           DFEN+HRDKVRGWVGFS+KMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVH VG
Sbjct: 661 DFENKHRDKVRGWVGFSIKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHRVG 720

Query: 745 GLRDTVIPFDPFNESGFGWTFDSAEANKLIHALGNCLLTYRQYKKSWEGLQRRGMTQDLS 804
           GLRDTVIPFDPFNESG+GWTFDSAEANKLIHALGNCLLTYR+YKKSWEGLQ RGMTQDLS
Sbjct: 721 GLRDTVIPFDPFNESGYGWTFDSAEANKLIHALGNCLLTYREYKKSWEGLQIRGMTQDLS 780

Query: 805 WDHAAERYEEVLVAAKYQW 819
           WDHAAE+YEEVLVAAKYQW
Sbjct: 781 WDHAAEKYEEVLVAAKYQW 796

BLAST of Cp4.1LG03g08100 vs. TAIR 10
Match: AT3G01180.1 (starch synthase 2 )

HSP 1 Score: 941.4 bits (2432), Expect = 4.8e-274
Identity = 477/732 (65.16%), Postives = 560/732 (76.50%), Query Frame = 0

Query: 98  SGQRSDDPDQAIQATIEKSKKVLAMQQQLLEQIAERKKLVSSIKGTSLDSKEEQNSNEQM 157
           SG   D+P+ A+QATI+KSKKVLAMQ+ LL QIAER+KLVSSIK ++ D  + + S++Q 
Sbjct: 63  SGSDDDEPEDALQATIDKSKKVLAMQRNLLHQIAERRKLVSSIKESTPDLDDAKASSKQE 122

Query: 158 DGSFPIPDFPSRNDEGTVDSLNGSSLPVSNDNLDVRKQLDAVTTFSSRPSDKNDEK---Q 217
             S    +  +   E      NGS  P +     + K+ +A T   S  S KN ++    
Sbjct: 123 SASSVNANTDATKKEIMDGDANGSVSPSTYGKSSLSKEPEAKTFSPSTESLKNRKQSSAS 182

Query: 218 VVDLKKIISSAGPKDQL---KSSSKIKYSDVLPNRKDQLKGSSKKVLPDSKHQLKGSSKK 277
           V+    + S   P D     K  S +  S V P  K     +S +   D        SK 
Sbjct: 183 VISSSPVTSPQKPSDVATNGKPWSSVVASSVDPPYKPSSVMTSPEKTSDPVTSPGKPSKS 242

Query: 278 KS----SDFVPAFLASKSSYSAVKEEKNGQPSEPTLKVDSDAAESESVDVKPSPLAGVNV 337
           ++    SD +P++L      S +K EK  + +       S+  E    + KP PLAG NV
Sbjct: 243 RAGAFWSDPLPSYLTKAPQTSTMKTEKYVEKTPDV--ASSETNEPGKDEEKPPPLAGANV 302

Query: 338 MNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGVRKRYK 397
           MNVI+VAAECAP+SKTGGLGDVAG+LPK+LARRGHRVMV+VPRYA YA  +D GVRKRYK
Sbjct: 303 MNVILVAAECAPFSKTGGLGDVAGALPKSLARRGHRVMVVVPRYAEYAEAKDLGVRKRYK 362

Query: 398 VDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYGGDHQDILKRMTFFCKAAVEVPWH 457
           V GQD+EV +F  ++DGVDFVFID+  F H   NIYGG+  DILKRM  FCKAAVEVPW+
Sbjct: 363 VAGQDMEVMYFHAFIDGVDFVFIDSPEFRHLSNNIYGGNRLDILKRMVLFCKAAVEVPWY 422

Query: 458 VPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHNIAHQGRGPVD 517
           VPCGGVCYGDGNL F+ANDWHTA+LPVYLKAYYRD+G+M +TRS+LVIHNIAHQGRGPVD
Sbjct: 423 VPCGGVCYGDGNLAFIANDWHTALLPVYLKAYYRDHGIMKYTRSVLVIHNIAHQGRGPVD 482

Query: 518 DFYRVDLPEHYMDLFKMYDPVGGEHFNIFAAGLKAADRVVTVSHGYAWELKTIEGGWGLH 577
           DF  VDLP HY+D FK+YDPVGGEHFNIFAAGLKAADRV+TVSHGY+WE+KT+EGGWGLH
Sbjct: 483 DFSYVDLPSHYLDSFKLYDPVGGEHFNIFAAGLKAADRVLTVSHGYSWEVKTLEGGWGLH 542

Query: 578 GIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTNYSIETLQTGKPQCKAALQKELGL 637
            IINE DWK  GIVNGID++EW+P++D +L SD YTNYS+E L  GKPQCKAALQKELGL
Sbjct: 543 NIINENDWKFRGIVNGIDTQEWNPEFDTYLHSDDYTNYSLENLHIGKPQCKAALQKELGL 602

Query: 638 PVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQLVLLGTGRQDLEQMLRDFENQHR 697
           PVR DVPLIGFIGRLDHQKGVD+IAEA+PWM+ QDVQLV+LGTGR DLE++LR  E+Q+R
Sbjct: 603 PVRPDVPLIGFIGRLDHQKGVDLIAEAVPWMMSQDVQLVMLGTGRPDLEEVLRQMEHQYR 662

Query: 698 DKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGTIPVVHSVGGLRDTVI 757
           DK RGWVGFSVK AHRITAG DILLMPSRFEPCGLNQLYAMNYGTIPVVH+VGGLRDTV 
Sbjct: 663 DKARGWVGFSVKTAHRITAGADILLMPSRFEPCGLNQLYAMNYGTIPVVHAVGGLRDTVQ 722

Query: 758 PFDPFNESGFGWTFDSAEANKLIHALGNCLLTYRQYKKSWEGLQRRGMTQDLSWDHAAER 817
            FDP++E+G GWTFDSAEA KLIHALGNCLLTYR+YK+SWEGLQRRGMTQDLSWD+AAE+
Sbjct: 723 QFDPYSETGLGWTFDSAEAGKLIHALGNCLLTYREYKESWEGLQRRGMTQDLSWDNAAEK 782

Query: 818 YEEVLVAAKYQW 820
           YEEVLVAAKY W
Sbjct: 783 YEEVLVAAKYHW 792

BLAST of Cp4.1LG03g08100 vs. TAIR 10
Match: AT5G24300.1 (Glycogen/starch synthases, ADP-glucose type )

HSP 1 Score: 485.3 bits (1248), Expect = 9.4e-137
Identity = 254/523 (48.57%), Postives = 330/523 (63.10%), Query Frame = 0

Query: 319 PSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRY------- 378
           PS  A V V N++ V +E AP+SKTGGLGDV GSLP ALA RGHRVMVI PRY       
Sbjct: 134 PSGKAEV-VNNLVFVTSEAAPYSKTGGLGDVCGSLPIALAGRGHRVMVISPRYLNGTAAD 193

Query: 379 ANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYG---GDHQ 438
            NYA  +D G+R      G   EV+F+  Y DGVD+VF+D+  + HR  N YG   G   
Sbjct: 194 KNYARAKDLGIRVTVNCFGGSQEVSFYHEYRDGVDWVFVDHKSY-HRPGNPYGDSKGAFG 253

Query: 439 DILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIF 498
           D   R T  C AA E P  +P GG  YG+ +L F+ NDWH  ++P+ L A YR  G+   
Sbjct: 254 DNQFRFTLLCHAACEAPLVLPLGGFTYGEKSL-FLVNDWHAGLVPILLAAKYRPYGVYKD 313

Query: 499 TRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPV--------GGEHFNIFAAGL 558
            RSIL+IHN+AHQG  P   +  + LP  +        P          GE  N+    +
Sbjct: 314 ARSILIIHNLAHQGVEPAATYTNLGLPSEWYGAVGWVFPTWARTHALDTGEAVNVLKGAI 373

Query: 559 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 618
             +DR++TVS GYAWE+ T+EGG+GL  +++     + GI NGI+  EW+P  D H+   
Sbjct: 374 VTSDRIITVSQGYAWEITTVEGGYGLQDLLSSRKSVINGITNGINVDEWNPSTDEHIP-- 433

Query: 619 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 678
              +YS + + + K +CK ALQKELGLP+R + P+IGFIGRLD+QKG+D+I  A P ++ 
Sbjct: 434 --FHYSADDV-SEKIKCKMALQKELGLPIRPECPMIGFIGRLDYQKGIDLIQTAGPDLMV 493

Query: 679 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 738
            D+Q V+LG+G    E  +R  E  +RDK RGWVGF+V ++HRITAG DILLMPSRFEPC
Sbjct: 494 DDIQFVMLGSGDPKYESWMRSMEETYRDKFRGWVGFNVPISHRITAGCDILLMPSRFEPC 553

Query: 739 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNE----SGFGWTFDSAEANKLIHALGNC 798
           GLNQLYAM YGTIPVVH  GGLRDTV  F+P+ E    +G GW F     + ++ AL   
Sbjct: 554 GLNQLYAMRYGTIPVVHGTGGLRDTVENFNPYAEGGAGTGTGWVFTPLSKDSMVSALRLA 613

Query: 799 LLTYRQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 820
             TYR+YK+SWEGL RRGMT++ SW++AA +YE+V     +QW
Sbjct: 614 AATYREYKQSWEGLMRRGMTRNYSWENAAVQYEQV-----FQW 643

BLAST of Cp4.1LG03g08100 vs. TAIR 10
Match: AT5G24300.2 (Glycogen/starch synthases, ADP-glucose type )

HSP 1 Score: 485.3 bits (1248), Expect = 9.4e-137
Identity = 254/523 (48.57%), Postives = 330/523 (63.10%), Query Frame = 0

Query: 319 PSPLAGVNVMNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRY------- 378
           PS  A V V N++ V +E AP+SKTGGLGDV GSLP ALA RGHRVMVI PRY       
Sbjct: 134 PSGKAEV-VNNLVFVTSEAAPYSKTGGLGDVCGSLPIALAGRGHRVMVISPRYLNGTAAD 193

Query: 379 ANYAGLQDTGVRKRYKVDGQDLEVAFFQTYVDGVDFVFIDNNMFHHREQNIYG---GDHQ 438
            NYA  +D G+R      G   EV+F+  Y DGVD+VF+D+  + HR  N YG   G   
Sbjct: 194 KNYARAKDLGIRVTVNCFGGSQEVSFYHEYRDGVDWVFVDHKSY-HRPGNPYGDSKGAFG 253

Query: 439 DILKRMTFFCKAAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIF 498
           D   R T  C AA E P  +P GG  YG+ +L F+ NDWH  ++P+ L A YR  G+   
Sbjct: 254 DNQFRFTLLCHAACEAPLVLPLGGFTYGEKSL-FLVNDWHAGLVPILLAAKYRPYGVYKD 313

Query: 499 TRSILVIHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYDPV--------GGEHFNIFAAGL 558
            RSIL+IHN+AHQG  P   +  + LP  +        P          GE  N+    +
Sbjct: 314 ARSILIIHNLAHQGVEPAATYTNLGLPSEWYGAVGWVFPTWARTHALDTGEAVNVLKGAI 373

Query: 559 KAADRVVTVSHGYAWELKTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSD 618
             +DR++TVS GYAWE+ T+EGG+GL  +++     + GI NGI+  EW+P  D H+   
Sbjct: 374 VTSDRIITVSQGYAWEITTVEGGYGLQDLLSSRKSVINGITNGINVDEWNPSTDEHIP-- 433

Query: 619 GYTNYSIETLQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVG 678
              +YS + + + K +CK ALQKELGLP+R + P+IGFIGRLD+QKG+D+I  A P ++ 
Sbjct: 434 --FHYSADDV-SEKIKCKMALQKELGLPIRPECPMIGFIGRLDYQKGIDLIQTAGPDLMV 493

Query: 679 QDVQLVLLGTGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPC 738
            D+Q V+LG+G    E  +R  E  +RDK RGWVGF+V ++HRITAG DILLMPSRFEPC
Sbjct: 494 DDIQFVMLGSGDPKYESWMRSMEETYRDKFRGWVGFNVPISHRITAGCDILLMPSRFEPC 553

Query: 739 GLNQLYAMNYGTIPVVHSVGGLRDTVIPFDPFNE----SGFGWTFDSAEANKLIHALGNC 798
           GLNQLYAM YGTIPVVH  GGLRDTV  F+P+ E    +G GW F     + ++ AL   
Sbjct: 554 GLNQLYAMRYGTIPVVHGTGGLRDTVENFNPYAEGGAGTGTGWVFTPLSKDSMVSALRLA 613

Query: 799 LLTYRQYKKSWEGLQRRGMTQDLSWDHAAERYEEVLVAAKYQW 820
             TYR+YK+SWEGL RRGMT++ SW++AA +YE+V     +QW
Sbjct: 614 AATYREYKQSWEGLMRRGMTRNYSWENAAVQYEQV-----FQW 643

BLAST of Cp4.1LG03g08100 vs. TAIR 10
Match: AT1G32900.1 (UDP-Glycosyltransferase superfamily protein )

HSP 1 Score: 363.2 bits (931), Expect = 5.4e-100
Identity = 211/507 (41.62%), Postives = 287/507 (56.61%), Query Frame = 0

Query: 328 MNVIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRYANYAGLQDTGVRKRYK 387
           M+VI + AE  PWSKTGGLGDV G LP ALA RGHRVM I PRY  Y    DT V  + K
Sbjct: 84  MSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTICPRYDQYKDAWDTCVVVQIK 143

Query: 388 VDGQDLEVAFFQTYVDGVDFVFIDNNMFHHR-----EQNIYGG----DHQDILKRMTFFC 447
           V  +   V FF  Y  GVD VF+D+ +F  +        IYG     D+ D   R +  C
Sbjct: 144 VGDKVENVRFFHCYKRGVDRVFVDHPIFLAKVVGKTGSKIYGPITGVDYNDNQLRFSLLC 203

Query: 448 KAAVEVPWHVPCGGVCYGDG----NLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILV 507
           +AA+E P  +      Y  G    ++VFVANDWHTA+LP YLK+ Y+  G+ +  + +  
Sbjct: 204 QAALEAPQVLNLNSSKYFSGPYGEDVVFVANDWHTALLPCYLKSMYQSRGVYMNAKVVFC 263

Query: 508 IHNIAHQGRGPVDDFYRVDLPEHYMDLFKMYD----PVGGEHFNIFAAGLKAADRVVTVS 567
           IHNIA+QGR   DD+  ++LP  +   F   D    PV G   N   A +  A RV+TVS
Sbjct: 264 IHNIAYQGRFAFDDYSLLNLPISFKSSFDFMDGYEKPVKGRKINWMKAAILEAHRVLTVS 323

Query: 568 HGYAWEL-KTIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTNYSIET 627
             YA EL   ++ G  LH  +      ++GI+NG+D +EW+P  D ++       Y I T
Sbjct: 324 PYYAQELISGVDRGVELHKYLRM--KTVSGIINGMDVQEWNPSTDKYID----IKYDITT 383

Query: 628 LQTGKPQCKAALQKELGLPVREDVPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQLVLLG 687
           +   KP  K ALQ  +GLPV  DVP+IGFIGRL+ QKG DI+ EAI   +G +VQ+V+LG
Sbjct: 384 VTDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKFMGLNVQMVILG 443

Query: 688 TGRQDLEQMLRDFENQHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMN 747
           TG++ +E  + + E +   K  G   F+V +AH ITAG D +++PSRFEPCGL QL+AM 
Sbjct: 444 TGKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSRFEPCGLIQLHAMR 503

Query: 748 YGTIPVVHSVGGLRDTVIP-FDPFNESGFGWTFDSAEANKLIHALGNCLLTYRQYKKS-W 807
           YGT+P+V S GGL DTV   +  F+   F    +  + + +I            Y  S  
Sbjct: 504 YGTVPIVASTGGLVDTVKDGYTGFHIGRFNVKCEVVDPDDVIATAKAVTRAVAVYGTSAM 563

Query: 808 EGLQRRGMTQDLSWDHAAERYEEVLVA 815
           + + +  M QD SW   A  +E+VL++
Sbjct: 564 QEMVKNCMDQDFSWKGPARLWEKVLLS 584

BLAST of Cp4.1LG03g08100 vs. TAIR 10
Match: AT4G18240.1 (starch synthase 4 )

HSP 1 Score: 230.7 bits (587), Expect = 4.2e-60
Identity = 168/502 (33.47%), Postives = 251/502 (50.00%), Query Frame = 0

Query: 330  VIIVAAECAPWSKTGGLGDVAGSLPKALARRGHRVMVIVPRY--ANYAGLQ-----DTGV 389
            V+ +AAE AP +K GGLGDV   L KAL R+GH V +I+P+Y    Y  ++     DT V
Sbjct: 544  VVHIAAEMAPVAKVGGLGDVVAGLGKALQRKGHLVEIILPKYDCMQYDRVRDLRALDTVV 603

Query: 390  RKRYKVDGQDLEVAFFQTYVDGVDFVFID---NNMFHHREQNIYGGDHQDILKRMTFFCK 449
               +  DG+  +   +   V+G+   FI+    + F  R Q  YG   QD  +R ++F +
Sbjct: 604  ESYF--DGKLYKNKIWIGTVEGLPVHFIEPQHPSKFFWRGQ-FYG--EQDDFRRFSYFSR 663

Query: 450  AAVEVPWHVPCGGVCYGDGNLVFVANDWHTAMLPVYLKAYYRDNGLMIFTRSILVIHNIA 509
            AA+E+        +  G    +   +DW TA +       Y   GL    R     HN  
Sbjct: 664  AALELL-------LQSGKKPDIIHCHDWQTAFVAPLYWDLYAPKGL-DSARICFTCHNFE 723

Query: 510  HQGRGPVDDFYRVDLPEHYMDL-FKMYDPVGGEHFNIFAAGLKAADRVVTVSHGYAWELK 569
            +QG     +     L  + ++   +M D   G+  N     +  ++ V TVS  YA E++
Sbjct: 724  YQGTASASELGSCGLDVNQLNRPDRMQDHSSGDRVNPVKGAIIFSNIVTTVSPTYAQEVR 783

Query: 570  TIEGGWGLHGIINEIDWKMTGIVNGIDSKEWSPQYDIHLTSDGYTNYSIETLQTGKPQCK 629
            T EGG GLH  +N    K  GI+NGID+  W+P  D  L +     ++ + LQ GK + K
Sbjct: 784  TAEGGKGLHSTLNFHSKKFIGILNGIDTDSWNPATDPFLKA----QFNAKDLQ-GKEENK 843

Query: 630  AALQKELGLPVRED-VPLIGFIGRLDHQKGVDIIAEAIPWMVGQDVQLVLLGTGRQDLEQ 689
             AL+K+LGL   E   PL+G I RL  QKGV +I  AI   +    Q VLLG+    +  
Sbjct: 844  HALRKQLGLSSAESRRPLVGCITRLVPQKGVHLIRHAIYRTLELGGQFVLLGS--SPVPH 903

Query: 690  MLRDFEN-----QHRDKVRGWVGFSVKMAHRITAGIDILLMPSRFEPCGLNQLYAMNYGT 749
            + R+FE      +  D VR  + +   ++H I A  D+ ++PS FEPCGL Q+ AM YG+
Sbjct: 904  IQREFEGIEQQFKSHDHVRLLLKYDEALSHTIYAASDLFIIPSIFEPCGLTQMIAMRYGS 963

Query: 750  IPVVHSVGGLRDTVIPFDPF---NESGFGWTFDSAEANKLIHALGNCLLTYRQYKKSWEG 809
            IP+    GGL D+V   D      +   G+TF +A+     +AL      Y++ ++ W  
Sbjct: 964  IPIARKTGGLNDSVFDIDDDTIPTQFQNGFTFQTADEQGFNYALERAFNHYKKDEEKWMR 1023

Query: 810  LQRRGMTQDLSWDHAAERYEEV 812
            L  + M+ D SW  +A +YEE+
Sbjct: 1024 LVEKVMSIDFSWGSSATQYEEL 1025