Cp4.1LG03g08070 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g08070
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionaquaporin NIP2-1-like
LocationCp4.1LG03: 3002717 .. 3005286 (+)
RNA-Seq ExpressionCp4.1LG03g08070
SyntenyCp4.1LG03g08070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATAACACATCAGGGTTCTTCCCCCTTTGGCATTACTAAATTAAATAGCCAAACCTTGAGATCTTCCCACTTTCAAATACAATAAAAAGATGAGTTGTGTTCAAAATCCTGAGCTTCCCAACCCAGAAACTGTTGTCCATGTTCAAGATTTGGTCTCAATTGAAAACCCAGATTCCAAACACTCCATTTTTGCTTCTTTGTTTCAAAACCATTACCCTCCTGGGTTTTCCCGAAAGGTACCTAAACCCTTTTCTCACAATAACATGTTTTTTTTATTCCTATTTTTTTGGGTCTTTGATTACTGAACTGAACTCTTTTGTGTTTCCATAACCAAAACAGCTGGTGGCGGAGGTGATAGCCACGTACTTGCTAGTGTTTGTGACTTGTGGAGCAGCCGCATTGAGCGCCAGCGACGAACGGAGAGTCTCACAGCTTGGAGCCTCCGTGGCTGGCGGCCTCATCGTGACGGTAATGATCTACGCTGTCGGACACATTTCTGGCGCCCATATGAACCCGGCTGTCACCTTAGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTCCCACTCTCTATAACTCTTTTATTTCTTACTTAATTCAATATCCTAATCATTTCCTCTTTTTCTTAATTATTTAATACAAATTTAACATAACCACGTCTTTGTTGAGCATTGATATGCAAAGCACGAGAGTTTATGCTCAAACTGAACAACCGTGATTCCAAAATTTTCAAATTTAATATTAATATTTAGGATTTAGGGCGGTGCTGAGAATATGGGAACGAGGAGCCCTGTTCTTTATATTTATATATATATATATATATATATATATATATAAACTTTCTCTAATTTGTATTATTATTGTAGATATTGTTAGACGAACACAACTCTCCATAATGGTATGATATTGTCCACTATGAGCGTAAGCTCTCGTAGCTTTGCTTTGGACTTCCTCAAAAGACCCCATATGAATGGAGAGACTATTCTTTGTTTATAAACTCATGATCATTTCCTAAATTAGGTGATGTGAGACTTTCATCATCTAATAGATATAAATAAATAGGGGTAGATAGAAAAAAGAAGGAAGAACGCAGGAAAGCCGTTAAAATTGGTTTCGACTTTATTTGACAATAATTAAGAGAAGAATTTTGTAGAAATCAAAATAACAAAATATTGGTTTAGAAGCGTAGAAGAATGAGTAGAAAGGAGGAGGATGAGATAAAAGAGGGAAATGTAGGCCATGTTTAGTGGCCACATGATGTATGGACAAGCTACTCCAAATATTATTCTTAAGCCACGGCCACAAGGAGGAGAAGCTTGTGTGATCGCTTTGTGGCCACAACCTTGGGTTCATTTTTCCGACCAAAGAATGGGATGGGACATTTAGGCAGCATAATTCCAACACTCACTCAAATTTGGGATAGAGATAGAAATAGAGATAGAGATGTCCACCAAAAGTGGTTGCCCAATTGTGAGTGGAAGGTATACCAAAGGACAACACATATCTCTTCATGTGTGTTTTGATGGATATCAATTCACTTTTTCAAATTCCAATCAAGCAATCAAACAAATTATTTTAAATTCTTTATGAGTGATGTTGGTTTGGTTTGGTTTGGTTTGGTTTGGTTGGTAGGTTCCATTGTATGGAGCAGCACAATTGAGTGGTGCAACTTGTGCGGCCTTTACACTTCGCATATTATTGCATCCCATAAAACAATTGGGCACAACAACGCCATCGGGGACAGACTTTCAAGCATTGGTCATGGAGATTGTGGTTACTTTCTCAATGATGTTCGTCACATCCGCTGTTGCAACTGACACTAAAGCAGTAAGAATCAATGACCAATGCTTTCAATTTCATGTTTTGATTTGATTCGATTCATTTGAGAAATGGTTGTTTGTTTGGCAGATAGGAGAGCTAGCCGGTATGGCAGTTGGGTCAGCTGTGTGTATCACTTCCATCTTAGCTGGGTAAGGAAACATAGACATTTTTTTGGTTCGACTAACTCTTTTTCTTTTTAAAAAGTGATTAAAAAAAAATCCGTTTTGCATATGGACATATTACATTAAACAAATGAATCTAATGAGGGTTTGTGTTAGCGACTATTCAGACCTGTATCAGGAGGGTCAATGAATCCTGTGAGGACATTAGGACCTGCATTGGCAAGTGAATATTATAAAGGACTTTGGGTGTACTTTGTTGGGCCGGTTACTGGAACTCTATTGGGGGCATGGTCATATAAATTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCTCACTCATTTTCATTCAAACTTCGAAGAATGTCAAGATCAGATGTTAGTGAGGGTGGAAAGTGAGCGTTGGCTCCTCATCTTCTTCCATCTCTAAAATATTTGGAATGTTGGAAAAGATGTGAAATAGTTTGAGTGTTTCATGAGCATGATCTCGTGTCGTGGTGTACTATGCTATAAGCTTCTTTATTATAGCTTAGCGATGTATGAGAAGTAGTGATTGTAACCTCATCTTCTATGTATGAAAATTTTCTACCTTTGGTTACTTTTGTTCTTTTTAGTTC

mRNA sequence

TATAACACATCAGGGTTCTTCCCCCTTTGGCATTACTAAATTAAATAGCCAAACCTTGAGATCTTCCCACTTTCAAATACAATAAAAAGATGAGTTGTGTTCAAAATCCTGAGCTTCCCAACCCAGAAACTGTTGTCCATGTTCAAGATTTGGTCTCAATTGAAAACCCAGATTCCAAACACTCCATTTTTGCTTCTTTGTTTCAAAACCATTACCCTCCTGGGTTTTCCCGAAAGCTGGTGGCGGAGGTGATAGCCACGTACTTGCTAGTGTTTGTGACTTGTGGAGCAGCCGCATTGAGCGCCAGCGACGAACGGAGAGTCTCACAGCTTGGAGCCTCCGTGGCTGGCGGCCTCATCGTGACGGTAATGATCTACGCTGTCGGACACATTTCTGGCGCCCATATGAACCCGGCTGTCACCTTAGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTTCCATTGTATGGAGCAGCACAATTGAGTGGTGCAACTTGTGCGGCCTTTACACTTCGCATATTATTGCATCCCATAAAACAATTGGGCACAACAACGCCATCGGGGACAGACTTTCAAGCATTGGTCATGGAGATTGTGGTTACTTTCTCAATGATGTTCGTCACATCCGCTGTTGCAACTGACACTAAAGCAATAGGAGAGCTAGCCGGTATGGCAGTTGGGTCAGCTGTGTGTATCACTTCCATCTTAGCTGGACCTGTATCAGGAGGGTCAATGAATCCTGTGAGGACATTAGGACCTGCATTGGCAAGTGAATATTATAAAGGACTTTGGGTGTACTTTGTTGGGCCGGTTACTGGAACTCTATTGGGGGCATGGTCATATAAATTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCTCACTCATTTTCATTCAAACTTCGAAGAATGTCAAGATCAGATGTTAGTGAGGGTGGAAAGTGAGCGTTGGCTCCTCATCTTCTTCCATCTCTAAAATATTTGGAATGTTGGAAAAGATGTGAAATAGTTTGAGTGTTTCATGAGCATGATCTCGTGTCGTGGTGTACTATGCTATAAGCTTCTTTATTATAGCTTAGCGATGTATGAGAAGTAGTGATTGTAACCTCATCTTCTATGTATGAAAATTTTCTACCTTTGGTTACTTTTGTTCTTTTTAGTTC

Coding sequence (CDS)

ATGAGTTGTGTTCAAAATCCTGAGCTTCCCAACCCAGAAACTGTTGTCCATGTTCAAGATTTGGTCTCAATTGAAAACCCAGATTCCAAACACTCCATTTTTGCTTCTTTGTTTCAAAACCATTACCCTCCTGGGTTTTCCCGAAAGCTGGTGGCGGAGGTGATAGCCACGTACTTGCTAGTGTTTGTGACTTGTGGAGCAGCCGCATTGAGCGCCAGCGACGAACGGAGAGTCTCACAGCTTGGAGCCTCCGTGGCTGGCGGCCTCATCGTGACGGTAATGATCTACGCTGTCGGACACATTTCTGGCGCCCATATGAACCCGGCTGTCACCTTAGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTTCCATTGTATGGAGCAGCACAATTGAGTGGTGCAACTTGTGCGGCCTTTACACTTCGCATATTATTGCATCCCATAAAACAATTGGGCACAACAACGCCATCGGGGACAGACTTTCAAGCATTGGTCATGGAGATTGTGGTTACTTTCTCAATGATGTTCGTCACATCCGCTGTTGCAACTGACACTAAAGCAATAGGAGAGCTAGCCGGTATGGCAGTTGGGTCAGCTGTGTGTATCACTTCCATCTTAGCTGGACCTGTATCAGGAGGGTCAATGAATCCTGTGAGGACATTAGGACCTGCATTGGCAAGTGAATATTATAAAGGACTTTGGGTGTACTTTGTTGGGCCGGTTACTGGAACTCTATTGGGGGCATGGTCATATAAATTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCTCACTCATTTTCATTCAAACTTCGAAGAATGTCAAGATCAGATGTTAGTGAGGGTGGAAAGTGA

Protein sequence

MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK
Homology
BLAST of Cp4.1LG03g08070 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 328.9 bits (842), Expect = 5.6e-89
Identity = 168/268 (62.69%), Postives = 207/268 (77.24%), Query Frame = 0

Query: 18  VQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDERR 77
           + D+ + +N     + +     + +PP   +K+V+EV++T+LLVFVTCGAA +  SD+ R
Sbjct: 17  IHDIGTAQNSSMPPTYYDRSLADIFPPHLLKKVVSEVVSTFLLVFVTCGAAGIYGSDKDR 76

Query: 78  VSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGAT 137
           +SQLG SVAGGLIVTVMIYAVGHISGAHMNPAVTLAFA  RHFPW QVP Y AAQ +G+ 
Sbjct: 77  ISQLGQSVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGSI 136

Query: 138 CAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMA 197
           CA+F L+ +LHPI  LGTTTP+G  + +LV+EI+VTF+MMFVT AVATDT+A+GELAG+A
Sbjct: 137 CASFVLKAVLHPIAVLGTTTPTGPHWHSLVIEIIVTFNMMFVTLAVATDTRAVGELAGLA 196

Query: 198 VGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIR 257
           VGSAVCITSI AG VSGGSMNP RTLGPALAS  Y GLW+YF+GPV GTL GAW+Y +IR
Sbjct: 197 VGSAVCITSIFAGAVSGGSMNPARTLGPALASNLYTGLWIYFLGPVLGTLSGAWTYTYIR 256

Query: 258 ASDKPVHL-ISPHSFSFKLRRMSRSDVS 285
             + P H  +S    SFKLRR+    V+
Sbjct: 257 FEEAPSHKDMSQKLSSFKLRRLQSQSVA 284

BLAST of Cp4.1LG03g08070 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.1e-87
Identity = 164/262 (62.60%), Postives = 202/262 (77.10%), Query Frame = 0

Query: 18  VQDLVSIENPDSKHSIFA-SLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDER 77
           + DL +++N       +      + +PP   +K+V+EV+AT+LLVF+TCGAA +S SD  
Sbjct: 18  IHDLSTVQNGTMPTMYYGEKAIADFFPPHLLKKVVSEVVATFLLVFMTCGAAGISGSDLS 77

Query: 78  RVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGA 137
           R+SQLG S+AGGLIVTVMIYAVGHISGAHMNPAVTLAFA  RHFPW QVP Y AAQ +GA
Sbjct: 78  RISQLGQSIAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGA 137

Query: 138 TCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGM 197
            CA+F L+ ++HP+  +GTTTP G  + +LV+E++VTF+MMFVT AVATDT+A+GELAG+
Sbjct: 138 ICASFVLKAVIHPVDVIGTTTPVGPHWHSLVVEVIVTFNMMFVTLAVATDTRAVGELAGL 197

Query: 198 AVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFI 257
           AVGSAVCITSI AG +SGGSMNP RTLGPALAS  + GLW+YF+GPV GTL GAW+Y FI
Sbjct: 198 AVGSAVCITSIFAGAISGGSMNPARTLGPALASNKFDGLWIYFLGPVMGTLSGAWTYTFI 257

Query: 258 RASDKPVHLISPHSFSFKLRRM 279
           R  D P    S    SFKLRR+
Sbjct: 258 RFEDTPKEGSSQKLSSFKLRRL 279

BLAST of Cp4.1LG03g08070 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 1.1e-87
Identity = 162/237 (68.35%), Postives = 192/237 (81.01%), Query Frame = 0

Query: 42  YPPGFSRKLVAEVIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHI 101
           +PP   +K+++EV+AT+LLVFVTCGAA++   D RR+SQLG SVAGGLIVTVMIYA GHI
Sbjct: 46  FPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNRRISQLGQSVAGGLIVTVMIYATGHI 105

Query: 102 SGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGT 161
           SGAHMNPAVTL+FA  RHFPW QVP Y AAQ +GA CAAF L+ +LHPI  +GTTTPSG 
Sbjct: 106 SGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLHPIAVIGTTTPSGP 165

Query: 162 DFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVR 221
            + AL++EIVVTF+MMFVT AVATD++A+GELAG+AVGSAVCITSI AGPVSGGSMNP R
Sbjct: 166 HWHALLIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNPAR 225

Query: 222 TLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRM 279
           TL PA+AS  + GLW+YF+GPV GTL GAW Y +IR  + P    +    SFKLRRM
Sbjct: 226 TLAPAVASNVFTGLWIYFLGPVIGTLSGAWVYTYIRFEEAPAAKDTQRLSSFKLRRM 282

BLAST of Cp4.1LG03g08070 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 321.6 bits (823), Expect = 8.9e-87
Identity = 166/272 (61.03%), Postives = 208/272 (76.47%), Query Frame = 0

Query: 18  VQDLVSIENPDSKHSIF--ASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDE 77
           + DL ++++  +  S++     F + +PP   +K+++EV+AT+LLVFVTCGAA++   D 
Sbjct: 20  IHDLSTVQSVSAVPSVYYPEKSFADIFPPNLLKKVISEVVATFLLVFVTCGAASIYGEDM 79

Query: 78  RRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSG 137
           +R+SQLG SV GGLIVTVMIYA GHISGAHMNPAVTL+FA  RHFPW QVP Y AAQ +G
Sbjct: 80  KRISQLGQSVVGGLIVTVMIYATGHISGAHMNPAVTLSFAFFRHFPWIQVPFYWAAQFTG 139

Query: 138 ATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAG 197
           A CAAF LR +L+PI+ LGTTTP+G  + ALV+EIVVTF+MMFVT AVATD++A+GELAG
Sbjct: 140 AMCAAFVLRAVLYPIEVLGTTTPTGPHWHALVIEIVVTFNMMFVTCAVATDSRAVGELAG 199

Query: 198 MAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKF 257
           +AVGSAVCITSI AGPVSGGSMNP RTL PA+AS  Y GLW+YF+GPV GTL GAW Y +
Sbjct: 200 LAVGSAVCITSIFAGPVSGGSMNPARTLAPAVASNVYTGLWIYFLGPVVGTLSGAWVYTY 259

Query: 258 IRASDKPVHL--ISPHSF-SFKLRRMSRSDVS 285
           IR  + P      +P    SFKLRR+    ++
Sbjct: 260 IRFEEAPAAAGGAAPQKLSSFKLRRLQSQSMA 291

BLAST of Cp4.1LG03g08070 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 8.3e-85
Identity = 166/254 (65.35%), Postives = 195/254 (76.77%), Query Frame = 0

Query: 27  PDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSASDERRVSQLGASVA 86
           PDSK    A +F    PP   +K+++EV+AT+LLVFVTCGAA++   D  R+SQLG SVA
Sbjct: 40  PDSKS--IADIF----PPHLGKKVISEVVATFLLVFVTCGAASIYGEDNARISQLGQSVA 99

Query: 87  GGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRIL 146
           GGLIVTVMIYA GHISGAHMNPAVTL+FA  RHFPW QVP Y AAQ +GA CAAF L+ +
Sbjct: 100 GGLIVTVMIYATGHISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAV 159

Query: 147 LHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMAVGSAVCITS 206
           L PI  +GTTTPSG  + AL +EIVVTF+MMFVT AVATD++A+GELAG+AVGSAVCITS
Sbjct: 160 LQPIAVIGTTTPSGPHWHALAIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITS 219

Query: 207 ILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIRASDKPVHL- 266
           I AGPVSGGSMNP RTL PA+AS  + GLW+YF+GPV GTL GAW Y +IR  + P    
Sbjct: 220 IFAGPVSGGSMNPARTLAPAVASNVFTGLWIYFLGPVVGTLSGAWVYTYIRFEEAPAAAK 279

Query: 267 -ISPHSFSFKLRRM 279
             +    SFKLRRM
Sbjct: 280 PDTQRLSSFKLRRM 287

BLAST of Cp4.1LG03g08070 vs. NCBI nr
Match: XP_023526670.1 (aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563 bits (1450), Expect = 5.23e-202
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
           SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288
           GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288

BLAST of Cp4.1LG03g08070 vs. NCBI nr
Match: XP_022934054.1 (aquaporin NIP2-1-like [Cucurbita moschata])

HSP 1 Score: 562 bits (1449), Expect = 7.43e-202
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MSCVQNPELPNPETVVHVQDLVS+ENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSVENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
           SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288
           GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288

BLAST of Cp4.1LG03g08070 vs. NCBI nr
Match: XP_022983845.1 (aquaporin NIP2-1-like [Cucurbita maxima])

HSP 1 Score: 556 bits (1434), Expect = 1.44e-199
Identity = 285/288 (98.96%), Postives = 286/288 (99.31%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MSCVQNPELPNPETVVHVQDLVS+ENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSLENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
           SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288
           GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSE  K
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSESEK 288

BLAST of Cp4.1LG03g08070 vs. NCBI nr
Match: KAG7017705.1 (Aquaporin NIP2-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 558 bits (1439), Expect = 4.58e-197
Identity = 285/288 (98.96%), Postives = 287/288 (99.65%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MSCVQNPELPNPETVVHVQDLVS+ENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSVENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSG+DFQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGSDFQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
           SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288
           GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSE GK
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSESGK 288

BLAST of Cp4.1LG03g08070 vs. NCBI nr
Match: KAG6580962.1 (Aquaporin NIP2-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548 bits (1411), Expect = 5.97e-196
Identity = 282/295 (95.59%), Postives = 286/295 (96.95%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLV-------AE 60
           MSCVQNPELPNPETVVHVQDLVS+ENPDSKH+IFASLFQNHYPPGFSRK+         E
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSVENPDSKHTIFASLFQNHYPPGFSRKVPKPFSHTGGE 60

Query: 61  VIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLA 120
           VIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLA
Sbjct: 61  VIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLA 120

Query: 121 FAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVT 180
           FAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSG+DFQALVMEIVVT
Sbjct: 121 FAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGSDFQALVMEIVVT 180

Query: 181 FSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYK 240
           FSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYK
Sbjct: 181 FSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYK 240

Query: 241 GLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288
           GLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK
Sbjct: 241 GLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 295

BLAST of Cp4.1LG03g08070 vs. ExPASy TrEMBL
Match: A0A6J1F1I0 (aquaporin NIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111441338 PE=3 SV=1)

HSP 1 Score: 562 bits (1449), Expect = 3.60e-202
Identity = 287/288 (99.65%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MSCVQNPELPNPETVVHVQDLVS+ENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSVENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
           SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288
           GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288

BLAST of Cp4.1LG03g08070 vs. ExPASy TrEMBL
Match: A0A6J1J724 (aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482339 PE=3 SV=1)

HSP 1 Score: 556 bits (1434), Expect = 6.96e-200
Identity = 285/288 (98.96%), Postives = 286/288 (99.31%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MSCVQNPELPNPETVVHVQDLVS+ENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSCVQNPELPNPETVVHVQDLVSLENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF
Sbjct: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
           SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV
Sbjct: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEGGK 288
           GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSE  K
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSESEK 288

BLAST of Cp4.1LG03g08070 vs. ExPASy TrEMBL
Match: G8XUV3 (Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1)

HSP 1 Score: 496 bits (1276), Expect = 8.35e-176
Identity = 252/285 (88.42%), Postives = 266/285 (93.33%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MS +QNP+L N E VV V + VS+ENPDSK S F S F+N YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAAL+ASD RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT+AFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLR+LLHPIK LGTTTPSG+D QALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
            AVATDTKA+GELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALAS+YYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSE 285
           GPV GT LGAWSYKFIRASDKPVHLISPHSFS K+RRMSRSDVSE
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSE 285

BLAST of Cp4.1LG03g08070 vs. ExPASy TrEMBL
Match: A0A1L7B7J0 (LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1)

HSP 1 Score: 496 bits (1276), Expect = 8.35e-176
Identity = 252/285 (88.42%), Postives = 266/285 (93.33%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MS +QNP+L N E VV V + VS+ENPDSK S F S F+N YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAAL+ASD RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT+AFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLR+LLHPIK LGTTTPSG+D QALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
            AVATDTKA+GELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALAS+YYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSE 285
           GPV GT LGAWSYKFIRASDKPVHLISPHSFS K+RRMSRSDVSE
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSE 285

BLAST of Cp4.1LG03g08070 vs. ExPASy TrEMBL
Match: Q7X9T0 (Nod26-like protein OS=Cucurbita pepo OX=3663 GN=NIP1 PE=2 SV=1)

HSP 1 Score: 491 bits (1263), Expect = 7.98e-174
Identity = 250/286 (87.41%), Postives = 266/286 (93.01%), Query Frame = 0

Query: 1   MSCVQNPELPNPETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLL 60
           MS  Q+P+L   ++VV V++ VS+ENPDSK S F SLF+NHYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSSQDPQLVQQQSVVDVEEFVSVENPDSKRSQFGSLFKNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHF 120
           VFVTCGAAAL+ SD +RVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVT AFAATRHF
Sbjct: 61  VFVTCGAAALNGSDAQRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTTAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQALVMEIVVTFSMMFVT 180
           PWKQVPLY AAQLSGATCAAFTLR+LLHPIK LGTTTPSG+D QALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYAAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 SAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFV 240
            AVATDTKA+GELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPA+AS+ YKGLWVYFV
Sbjct: 181 CAVATDTKAVGELAGLAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASDNYKGLWVYFV 240

Query: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSEG 286
           GPVTGTLLGAWSYKFIRASDKPVHLISPHSFS KLRRMSRSDV EG
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVGEG 286

BLAST of Cp4.1LG03g08070 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 219.9 bits (559), Expect = 2.6e-57
Identity = 116/244 (47.54%), Postives = 161/244 (65.98%), Query Frame = 0

Query: 46  FSRKLVAEVIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAH 105
           F +KL+AEV+ TY L+F  C A A++   ++ V+  G ++  GL V V++Y++GHISGAH
Sbjct: 50  FLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISGAH 109

Query: 106 MNPAVTLAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGT--------TT 165
            NPAVT+AFA+   FP KQVP Y  +Q+ G+T AA TLR+L    + + +        T 
Sbjct: 110 FNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGTL 169

Query: 166 PSGTDFQALVMEIVVTFSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSM 225
           PSG++ Q+ V+E ++TF +MFV S VATD +AIGELAG+AVGS V +  I+AGPVSG SM
Sbjct: 170 PSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGASM 229

Query: 226 NPVRTLGPALASEYYKGLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRR 282
           NP R+LGPA+    Y+GLW+Y V P+ G + GAW Y  +R +DKP+  I+      K  R
Sbjct: 230 NPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTDKPLREITKSGSFLKTVR 289

BLAST of Cp4.1LG03g08070 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 214.2 bits (544), Expect = 1.4e-55
Identity = 116/237 (48.95%), Postives = 159/237 (67.09%), Query Frame = 0

Query: 47  SRKLVAEVIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHM 106
           ++KL+AE+I TY ++F  CG   ++      ++  G  V  GLIV VMIY+ GHISGAH 
Sbjct: 42  TQKLIAEMIGTYFIIFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHF 101

Query: 107 NPAVTLAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPI-KQLGTTTPSGTDFQA 166
           NPAVT+ FA  R FPW QVPLY  AQL+G+  A+ TLR++ +   K    TTP+ +  QA
Sbjct: 102 NPAVTVTFAVFRRFPWYQVPLYIGAQLTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQA 161

Query: 167 LVMEIVVTFSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGP 226
           LV EI+++F +MFV S VATD++A GELAG+AVG  + +   +AGP+SG SMNP R+LGP
Sbjct: 162 LVAEIIISFLLMFVISGVATDSRATGELAGIAVGMTIILNVFVAGPISGASMNPARSLGP 221

Query: 227 ALASEYYKGLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSD 283
           A+    YKG+WVY VGP  G   G + Y F+R +DKP+  ++  S SF LR +++ D
Sbjct: 222 AIVMGRYKGIWVYIVGPFVGIFAGGFVYNFMRFTDKPLRELT-KSASF-LRSVAQKD 276

BLAST of Cp4.1LG03g08070 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 211.5 bits (537), Expect = 9.2e-55
Identity = 121/275 (44.00%), Postives = 169/275 (61.45%), Query Frame = 0

Query: 13  ETVVHVQDLVSIENPDSKHSIFASLFQNHYPPGFSRKLVAEVIATYLLVFVTCGAAALSA 72
           + V H Q++  I NP        SL     P  F +KL+AE + TY LVF  C +  ++ 
Sbjct: 23  DEVEHQQEMEDIHNPRPLKK-QDSLLSVSVP--FLQKLIAEFLGTYFLVFTGCASVVVNM 82

Query: 73  SDERRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAATRHFPWKQVPLYGAAQ 132
            ++  V+  G ++  GL + V+IY++GHISGAH+NPAVT+AFA+   FP KQVP Y  +Q
Sbjct: 83  QNDNVVTLPGIAIVWGLTIMVLIYSLGHISGAHINPAVTIAFASCGRFPLKQVPAYVISQ 142

Query: 133 LSGATCAAFTLRILL---HPIKQ------LGTTTPSGTDFQALVMEIVVTFSMMFVTSAV 192
           + G+T AA TLR+L    H +        +G ++P G+D QA  ME +VTF +MF+ S V
Sbjct: 143 VIGSTLAAATLRLLFGLDHDVCSGKHDVFIG-SSPVGSDLQAFTMEFIVTFYLMFIISGV 202

Query: 193 ATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASEYYKGLWVYFVGPV 252
           ATD +AIGELAG+A+GS V +  ++A PVS  SMNP R+LGPAL    YKG+W+Y V P 
Sbjct: 203 ATDNRAIGELAGLAIGSTVLLNVLIAAPVSSASMNPGRSLGPALVYGCYKGIWIYLVAPT 262

Query: 253 TGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRM 279
            G + GAW Y  +R +DKP+  I+      K  R+
Sbjct: 263 LGAIAGAWVYNTVRYTDKPLREITKSGSFLKTVRI 293

BLAST of Cp4.1LG03g08070 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 210.3 bits (534), Expect = 2.1e-54
Identity = 117/243 (48.15%), Postives = 157/243 (64.61%), Query Frame = 0

Query: 47  SRKLVAEVIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHM 106
           ++KL+AE+I TY +VF  CG   ++      ++  G  V  GLIV VMIY+ GHISGAH 
Sbjct: 42  TQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHF 101

Query: 107 NPAVTLAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQ-LGTTTPSGTDFQA 166
           NPAVT+ FA  R FPW QVPLY  AQ +G+  A+ TLR++     +    TTP+ +  +A
Sbjct: 102 NPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFGTTPADSPARA 161

Query: 167 LVMEIVVTFSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGP 226
           LV EI+++F +MFV S VATD +A+GELAG+AVG  + +   +AGP+SG SMNP R+LGP
Sbjct: 162 LVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLGP 221

Query: 227 ALASEYYKGLWVYFVGPVTGTLLGAWSYKFIRASDKPVHLISPHSFSFKLRRMSRSDVSE 286
           AL    YK +WVY VGPV G + G + Y  IR +DKP+  ++  S SF LR +S S    
Sbjct: 222 ALVMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDKPLRELT-KSASF-LRAVSPSHKGS 281

Query: 287 GGK 289
             K
Sbjct: 282 SSK 282

BLAST of Cp4.1LG03g08070 vs. TAIR 10
Match: AT4G10380.1 (NOD26-like intrinsic protein 5;1 )

HSP 1 Score: 205.7 bits (522), Expect = 5.1e-53
Identity = 102/214 (47.66%), Postives = 146/214 (68.22%), Query Frame = 0

Query: 47  SRKLVAEVIATYLLVFVTCGAAALSASDERRVSQLGASVAGGLIVTVMIYAVGHISGAHM 106
           +RKL AE + T++L+F       ++   +   + +G +   GL V ++I + GHISGAH+
Sbjct: 77  TRKLGAEFVGTFILIFTATAGPIVNQKYDGAETLIGNAACAGLAVMIIILSTGHISGAHL 136

Query: 107 NPAVTLAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRILLHPIKQLGTTTPSGTDFQAL 166
           NP++T+AFAA RHFPW  VP Y AAQ+S + CA+F L+ + HP    G T PS +  QA 
Sbjct: 137 NPSLTIAFAALRHFPWAHVPAYIAAQVSASICASFALKGVFHPFMSGGVTIPSVSLGQAF 196

Query: 167 VMEIVVTFSMMFVTSAVATDTKAIGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPA 226
            +E ++TF ++FV +AVATDT+A+GELAG+AVG+ V +  ++AGP +GGSMNPVRTLGPA
Sbjct: 197 ALEFIITFILLFVVTAVATDTRAVGELAGIAVGATVMLNILVAGPSTGGSMNPVRTLGPA 256

Query: 227 LASEYYKGLWVYFVGPVTGTLLGAWSYKFIRASD 261
           +AS  Y+ LWVY V P  G + GA  Y  ++ +D
Sbjct: 257 VASGNYRSLWVYLVAPTLGAISGAAVYTGVKLND 290

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q19KC15.6e-8962.69Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2[more]
Q6Z2T31.1e-8762.60Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1[more]
Q9ATN21.1e-8768.35Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1[more]
Q67WJ88.9e-8761.03Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1[more]
Q9AT748.3e-8565.35Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023526670.15.23e-202100.00aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo][more]
XP_022934054.17.43e-20299.65aquaporin NIP2-1-like [Cucurbita moschata][more]
XP_022983845.11.44e-19998.96aquaporin NIP2-1-like [Cucurbita maxima][more]
KAG7017705.14.58e-19798.96Aquaporin NIP2-1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6580962.15.97e-19695.59Aquaporin NIP2-1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1F1I03.60e-20299.65aquaporin NIP2-1-like OS=Cucurbita moschata OX=3662 GN=LOC111441338 PE=3 SV=1[more]
A0A6J1J7246.96e-20098.96aquaporin NIP2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111482339 PE=3 SV=1[more]
G8XUV38.35e-17688.42Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1[more]
A0A1L7B7J08.35e-17688.42LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1[more]
Q7X9T07.98e-17487.41Nod26-like protein OS=Cucurbita pepo OX=3663 GN=NIP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT4G18910.12.6e-5747.54NOD26-like intrinsic protein 1;2 [more]
AT5G37820.11.4e-5548.95NOD26-like intrinsic protein 4;2 [more]
AT4G19030.19.2e-5544.00NOD26-like major intrinsic protein 1 [more]
AT5G37810.12.1e-5448.15NOD26-like intrinsic protein 4;1 [more]
AT4G10380.15.1e-5347.66NOD26-like intrinsic protein 5;1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 199..221
score: 34.94
coord: 124..143
score: 36.12
coord: 48..67
score: 38.08
coord: 87..111
score: 50.67
coord: 168..186
score: 27.2
coord: 236..256
score: 36.26
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 46..253
e-value: 3.4E-53
score: 180.7
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 48..256
e-value: 6.53705E-56
score: 178.216
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 16..263
e-value: 3.3E-67
score: 228.1
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 42..266
NoneNo IPR availablePANTHERPTHR45724:SF30MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATEDcoord: 22..279
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 22..279
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 105..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g08070.1Cp4.1LG03g08070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity