Cp4.1LG03g07420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g07420
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionMitogen-activated protein kinase kinase 3
LocationCp4.1LG03: 3470754 .. 3475123 (+)
RNA-Seq ExpressionCp4.1LG03g07420
SyntenyCp4.1LG03g07420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTAACGAATAAAGAAGCTTTCAAAACCGACACTGGGTGGCGAAATTTTATTGGCGAGATCACAGGCGTGCCTAATAGCAAGCAAATCAGCAACCCGAATTTCCCGATTCGGCGCGCTGTGGGACATGCTCGAATCTCTCCGACCAGATTTATCGGAGGAAAGCGATTTCGGGGTTTTTGAAGAGATTTTTCGATTGTGAGTGAGTGATTCGATCGCGTTTTTTATCGTTATAATTTCACTATTCCAAATTCGCTTACAATTCTCTGTTTTGCTGTTCATTGTTCGTTTCAGTTCTAGCTTCTTTCCCCCACGATCAGTATCTCTAGTGTTGTACAGATTATTCGAACATGGCGGGTCTCGAGGAACTCAAGAGAAAACTTACTCCCTTGTTTGATGCTGAAAAGGGGCTTTCTATGGATTCCCCTGTGGACCCTTCTGATTCATATACGGTAACCTAATTGCAATTTAAATTTTAGAAAAGGGGTTTTTCTTTCTGATTGTTAAGAATATGAATTAATTTTGAGTTAGTGGATGATTTAACCTAACATCATAGTTGAAGGTCTTAATATTCGTAGTATTCATAGTTTGTGCTAGTTTTCAAGCCTGCAAATGAAGTGTGGATGAATAGAAATAAATGATTGACACTAATTGTTTGAGACCTTTATTGTTGTTTCTGAAGTTTTCAGACAATGGGACTGTTAATTTGCTGAGCCGATCATATGGGGTTTACAATTTTAATGAGCTAGGGTTACAAAAATGTACGTCGTGGCTTGCCGATGATTCGGGGAGTAGCGAAAGGACATATCGATGTGCTTCCCGTGAAATGAGGATATTTGGAGCCATTGGTAGTGGGGCTAGCAGTGTTGTTCAGAGAGCTATCCACATTCCTGCTCATCGAATCATGGCATTGAAGAAGATTAATATCTTTGAAAAGGTTTGTGATTTGGCATTCTCACTGTTTCTTTGCTTGTTGATGGTTTCTTTTAGCAATGACGCGTAATCGTGATGTGTTGAAGAGGTCATCTTTGAGATTCAGAACATGTTCTTGTTTTAAAGAGAGAATGTTTATGGTCCAGAAGGCAGCATAGTTGAAATTCATAATCTTAACGACATTTATGTTCCTTAACGACATTTATGTGTTGGGCTACCCTATATGTTCCTTAACGACATTTATGTTATGGAAATAGAAGCATATTGATGTCTTAAACTAGTGGCCACCATAGTTTGAGTCCACGACTTCAAAGATCGTATGAGTATAGAACATTTCAACTTGCTAACTATGCTAATCCATAGTGGTTTTGTTTAAGCCAATTCATATGCTTACTGTACTTCATTATTTTGTTTGCAGCTAATGGTACTATGATCTTTTAGTGTAAACAAATCTCAAATGAATTAGAAGTCATCCATCTTAATCTTTTTCTTATGTTTCTAGGAGAAAAGGCAGCAGCTTCTTACCGAGATAAGGACACTATGCGAAGCACCCTGTTCCGAGGGCCTTGTGGAGTTTCATGGAGCATTTTATACCCCTGATTCTGGGCAAATTAGCATAGCTTTGGAGTACATGGATGGAGGGTCGTTGGCAGATGTCCTGCGTTTGAAAAAATGTATACCCGAGCCTGTCCTTTCGTCTATGTTTCAGAAACTTCTTCAAGTATAGTTTTCTTTTCCCTTTCTCATTTATTATCCCCAAAATTCTGGGGTATATTTCCAATAATCGGCTTACTTGTTCTATTAACTAATTGGTACCATTTCATTCTGGTTTTTAAGGGTCTAAGTTATCTGCATGGAATGAGACATTTGGTTCACAGAGATATAAAGCCAGCCAATTTACTTGTAAATCTTAAGGGTGAAGCAAAAATTACTGATTTTGGCATAAGTGCTGGCTTGGAGAATTCAATGGCAATGGTTAGTTTTAATAGTTAGCATCGTTACCATTTCCCTCGGTGTTATTGACCATTGAATTGGAGGCAACCGAGACAAGTTTTTGTTAACTCGGTTTTTGCACGTGATAACAGTGTGCTACTTTCGTCGGAACTGTCACGTACATGTCACCAGAGCGAATTCGAAACGAGAGCTATTCTTATCCAGCTGACATTTGGAGCCTTGGGCTTGCACTCTTTGAGTGTGGTACTGGTGAATTTCCATATTCAGCTACTGAAGGTCTAGTGAACCTCATGTTGCAGGTATGAGCTTATGATCAAAAGATATCCTGCTTTTTCTTCTACGTGTGAATTACACCATTTCTGGAACTTATTTGTTCGGTGTACTTGACCAGATTGCCTTTTAACTTATTTAATGTTGCATTCTTTTTGTGTTTTCTAAACTCTTTTCCTTTACGATAGATTTTGGATGACCCGTCTCCATCGCCATCAAAACATAAGTTTTCTCCGGAGTTTTGCTCGTTTGTTGATGCTTGCTTGCAGAAAGACGCAGATGCTAGGCCAACAGCGGAGCAGGTAAGTCTATGCAAATTAACACGTCTTTTTAGATTTTGATCGTGCATGCTGGTTCAAGATGTATTCAATTACTAAAATCCAAATCAGAACTAGATTAATATTTTCAAAGATTTTTTGTTTTTGCTATGTAAAGACCTTATATCTCTTGAGGTAGGCAACACTGGTTCAAGATGGATAATGTTTGTTGATACGTCTCTGATTGCAGCTGCTTTCACACCCATTTATCAAAAAATATGAGGATGAACAAGTAGACTTGGCAGCTTTTGTACAGAATGTGTTTGATCCAATACAGAGGATGAAGGACTTGGCCGATGTAAGTATATAATATTTATGTTCTTAGTCTATTACCTATTAAACGACATTACCCTTACAAAATTACGAATATCATTCTAAGAGCCAGCCGATGTTCGGTCTGATCTCGTATGCAGTTTTTGAAATAGTCCTTTGGGTTCTTCTAGTATGAATATGGTGGAGCTGTGCTGGTGAAATAATCGTAGTCTTACAAGAACCTGAAGTCCAATATCTTAAACACCATTGTGAATTTTTGTAACAGCTTAAACCCATTGCTAACAGATATTGTCCTAAGCTTTTCCGATGGAGAGGGGAACGAAGCATTCCTTATAAGGGTGTGGAAAACTCTCCCTACCATACGTGTTTTAAAACCTTGAAGGGAAGCTTGAAAGGAAAAGCCGAAAGAGGACAATATCTACAAGCGGTGGGCTTGGGCTATTACACTTTTGCACGGTAAGACTGGATGCTGACCTCGTCTTTTTTTTTTTTTTCTTTCTTTATTTTCTCTTACCAGATGTTGACCATTCATTATTACTTGCTCTTTGATGGACCTGATGAATTATGGCACCATACAAAGGCTTTATACCATGAAAGCTCAACTTTAAGGTAGTGTGTTGTTGTTTATGTTTTACCTCCTTCTCTATAAACAATGGAGTTGGGAGACAATCCTCTTGATGGTTAAATTGGAATAAGTCACGACTCTTGCATACTTTTATTTTCAGTTTCTCAGGGAAGCAATTTTCTGGTCCAAATGATATCTTTGCCAAACTATCAGACATTCGAAGTACATTAGGGGGAGACTGGCCTCTTGAAAAACTAGTTCATGTCGTCGAGAAACTCCAATGCCGAGCTCACGGTCAGGATGGAATTGCCGTAAGGGTGTCTGGATCCTTCATTGTTGGGAACCAGTTCCTCATATGCGGAGACGGTGTACAAGTAGAGGGACTGCCAAATTTTAAGGATCTCTCCATTGACATGGCGAGTAAGAAAATGGGATCCTTCCGTGAGCAGTTCATCATCGAACCGAGTGACCTCATCGGCCGATACTTCATAGCCAAACAAGAGCTTTATATAATTCAATAGGAAAACGCCACTGCTGTTTGATCCTGCTGGTATGTTCAAGCCTCGACTCGTCTTATTATGCTTTATGATTCTAACTTAGAAGCATGATCCTTGTTGAGCTGTTGATAAGTTTATGCAACTCTTTGCAGTTTGTACAACTTTTTGTGAATCTGCAAACAAAAATGGATCAATCTCATCTGTAATTCCCATAGCAGAAATTATGTATCGAAATGTACTCGACCGTCGTAATTTAGCAGCCAGGGGTCGCAGCCGGTTCGTACACAAGATGAGTCAGTCATCTTCAAAGCAGCAGCACTTGAGGCCTCTCTTAGTGAGAATACTATATATGTCAAACTTGTTTAGTTATCTTCAGGATGAGTCTCTTAATTGTATATTTGTTTAGTTACAAATGTGTTATATATCACGTGCTAATTTTTTGTTATTGGTTTTTAATCATAGTTACTTTTTTCTTGTAAATTTTTTCATTTTTTTAAAACTTAATTTAAAATGTTGTTTTATTTTTCATTTATTATTTTAGGTTCTTAAATTAAATCAATATC

mRNA sequence

AGTAACGAATAAAGAAGCTTTCAAAACCGACACTGGGTGGCGAAATTTTATTGGCGAGATCACAGGCGTGCCTAATAGCAAGCAAATCAGCAACCCGAATTTCCCGATTCGGCGCGCTGTGGGACATGCTCGAATCTCTCCGACCAGATTTATCGGAGGAAAGCGATTTCGGGGTTTTTGAAGAGATTTTTCGATTTTCTAGCTTCTTTCCCCCACGATCAGTATCTCTAGTGTTGTACAGATTATTCGAACATGGCGGGTCTCGAGGAACTCAAGAGAAAACTTACTCCCTTGTTTGATGCTGAAAAGGGGCTTTCTATGGATTCCCCTGTGGACCCTTCTGATTCATATACGTTTTCAGACAATGGGACTGTTAATTTGCTGAGCCGATCATATGGGGTTTACAATTTTAATGAGCTAGGGTTACAAAAATGTACGTCGTGGCTTGCCGATGATTCGGGGAGTAGCGAAAGGACATATCGATGTGCTTCCCGTGAAATGAGGATATTTGGAGCCATTGGTAGTGGGGCTAGCAGTGTTGTTCAGAGAGCTATCCACATTCCTGCTCATCGAATCATGGCATTGAAGAAGATTAATATCTTTGAAAAGGAGAAAAGGCAGCAGCTTCTTACCGAGATAAGGACACTATGCGAAGCACCCTGTTCCGAGGGCCTTGTGGAGTTTCATGGAGCATTTTATACCCCTGATTCTGGGCAAATTAGCATAGCTTTGGAGTACATGGATGGAGGGTCGTTGGCAGATGTCCTGCGTTTGAAAAAATGTATACCCGAGCCTGTCCTTTCGTCTATGTTTCAGAAACTTCTTCAAGGTCTAAGTTATCTGCATGGAATGAGACATTTGGTTCACAGAGATATAAAGCCAGCCAATTTACTTGTAAATCTTAAGGGTGAAGCAAAAATTACTGATTTTGGCATAAGTGCTGGCTTGGAGAATTCAATGGCAATGTGTGCTACTTTCGTCGGAACTGTCACGTACATGTCACCAGAGCGAATTCGAAACGAGAGCTATTCTTATCCAGCTGACATTTGGAGCCTTGGGCTTGCACTCTTTGAGTGTGGTACTGGTGAATTTCCATATTCAGCTACTGAAGGTCTAGTGAACCTCATGTTGCAGATTTTGGATGACCCGTCTCCATCGCCATCAAAACATAAGTTTTCTCCGGAGTTTTGCTCGTTTGTTGATGCTTGCTTGCAGAAAGACGCAGATGCTAGGCCAACAGCGGAGCAGCTGCTTTCACACCCATTTATCAAAAAATATGAGGATGAACAAGTAGACTTGGCAGCTTTTGTACAGAATGTGTTTGATCCAATACAGAGGATGAAGGACTTGGCCGATGCTTTATACCATGAAAGCTCAACTTTAAGTTTCTCAGGGAAGCAATTTTCTGGTCCAAATGATATCTTTGCCAAACTATCAGACATTCGAAGTACATTAGGGGGAGACTGGCCTCTTGAAAAACTAGTTCATGTCGTCGAGAAACTCCAATGCCGAGCTCACGGTCAGGATGGAATTGCCGTAAGGGTGTCTGGATCCTTCATTGTTGGGAACCAGTTCCTCATATGCGGAGACGGTGTACAAGTAGAGGGACTGCCAAATTTTAAGGATCTCTCCATTGACATGGCGAGTAAGAAAATGGGATCCTTCCGTGAGCAGTTCATCATCGAACCGAGTGACCTCATCGGCCGATACTTCATAGCCAAACAAGAGCTTTATATAATTCAATAGGAAAACGCCACTGCTGTTTGATCCTGCTGTTTGTACAACTTTTTGTGAATCTGCAAACAAAAATGGATCAATCTCATCTGTAATTCCCATAGCAGAAATTATGTATCGAAATGTACTCGACCGTCGTAATTTAGCAGCCAGGGGTCGCAGCCGGTTCGTACACAAGATGAGTCAGTCATCTTCAAAGCAGCAGCACTTGAGGCCTCTCTTAGTGAGAATACTATATATGTCAAACTTGTTTAGTTATCTTCAGGATGAGTCTCTTAATTGTATATTTGTTTAGTTACAAATGTGTTATATATCACGTGCTAATTTTTTGTTATTGGTTTTTAATCATAGTTACTTTTTTCTTGTAAATTTTTTCATTTTTTTAAAACTTAATTTAAAATGTTGTTTTATTTTTCATTTATTATTTTAGGTTCTTAAATTAAATCAATATC

Coding sequence (CDS)

ATGGCGGGTCTCGAGGAACTCAAGAGAAAACTTACTCCCTTGTTTGATGCTGAAAAGGGGCTTTCTATGGATTCCCCTGTGGACCCTTCTGATTCATATACGTTTTCAGACAATGGGACTGTTAATTTGCTGAGCCGATCATATGGGGTTTACAATTTTAATGAGCTAGGGTTACAAAAATGTACGTCGTGGCTTGCCGATGATTCGGGGAGTAGCGAAAGGACATATCGATGTGCTTCCCGTGAAATGAGGATATTTGGAGCCATTGGTAGTGGGGCTAGCAGTGTTGTTCAGAGAGCTATCCACATTCCTGCTCATCGAATCATGGCATTGAAGAAGATTAATATCTTTGAAAAGGAGAAAAGGCAGCAGCTTCTTACCGAGATAAGGACACTATGCGAAGCACCCTGTTCCGAGGGCCTTGTGGAGTTTCATGGAGCATTTTATACCCCTGATTCTGGGCAAATTAGCATAGCTTTGGAGTACATGGATGGAGGGTCGTTGGCAGATGTCCTGCGTTTGAAAAAATGTATACCCGAGCCTGTCCTTTCGTCTATGTTTCAGAAACTTCTTCAAGGTCTAAGTTATCTGCATGGAATGAGACATTTGGTTCACAGAGATATAAAGCCAGCCAATTTACTTGTAAATCTTAAGGGTGAAGCAAAAATTACTGATTTTGGCATAAGTGCTGGCTTGGAGAATTCAATGGCAATGTGTGCTACTTTCGTCGGAACTGTCACGTACATGTCACCAGAGCGAATTCGAAACGAGAGCTATTCTTATCCAGCTGACATTTGGAGCCTTGGGCTTGCACTCTTTGAGTGTGGTACTGGTGAATTTCCATATTCAGCTACTGAAGGTCTAGTGAACCTCATGTTGCAGATTTTGGATGACCCGTCTCCATCGCCATCAAAACATAAGTTTTCTCCGGAGTTTTGCTCGTTTGTTGATGCTTGCTTGCAGAAAGACGCAGATGCTAGGCCAACAGCGGAGCAGCTGCTTTCACACCCATTTATCAAAAAATATGAGGATGAACAAGTAGACTTGGCAGCTTTTGTACAGAATGTGTTTGATCCAATACAGAGGATGAAGGACTTGGCCGATGCTTTATACCATGAAAGCTCAACTTTAAGTTTCTCAGGGAAGCAATTTTCTGGTCCAAATGATATCTTTGCCAAACTATCAGACATTCGAAGTACATTAGGGGGAGACTGGCCTCTTGAAAAACTAGTTCATGTCGTCGAGAAACTCCAATGCCGAGCTCACGGTCAGGATGGAATTGCCGTAAGGGTGTCTGGATCCTTCATTGTTGGGAACCAGTTCCTCATATGCGGAGACGGTGTACAAGTAGAGGGACTGCCAAATTTTAAGGATCTCTCCATTGACATGGCGAGTAAGAAAATGGGATCCTTCCGTGAGCAGTTCATCATCGAACCGAGTGACCTCATCGGCCGATACTTCATAGCCAAACAAGAGCTTTATATAATTCAATAG

Protein sequence

MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQKCTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKEKRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPSPSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPIQRMKDLADALYHESSTLSFSGKQFSGPNDIFAKLSDIRSTLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDLSIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ
Homology
BLAST of Cp4.1LG03g07420 vs. ExPASy Swiss-Prot
Match: O80396 (Mitogen-activated protein kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MKK3 PE=1 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 1.5e-230
Identity = 393/516 (76.16%), Postives = 440/516 (85.27%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MA LEELK+KL+PLFDAEKG S  S +DP+DSY  SD GTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAALEELKKKLSPLFDAEKGFSSSSSLDPNDSYLLSDGGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTS   D+S SSE TY+CAS EMR+FGAIGSGASSVVQRAIHIP HRI+ALKKINIFE+E
Sbjct: 61  CTSSHVDESESSETTYQCASHEMRVFGAIGSGASSVVQRAIHIPNHRILALKKINIFERE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPC EGLV+FHGAFY+PDSGQISIALEYM+GGSLAD+L++ K IPE
Sbjct: 121 KRQQLLTEIRTLCEAPCHEGLVDFHGAFYSPDSGQISIALEYMNGGSLADILKVTKKIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSS+F KLLQGLSYLHG+RHLVHRDIKPANLL+NLKGE KITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSLFHKLLQGLSYLHGVRHLVHRDIKPANLLINLKGEPKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRN+SYSYPADIWSLGLALFECGTGEFPY A EG VNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNDSYSYPADIWSLGLALFECGTGEFPYIANEGPVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           P+P K +FSPEFCSF+DACLQKD DARPTA+QLLSHPFI K+E E+VDLA FVQ++FDP 
Sbjct: 301 PTPPKQEFSPEFCSFIDACLQKDPDARPTADQLLSHPFITKHEKERVDLATFVQSIFDPT 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QR+KDLAD                     +LY E+S  SFSGK  +G  +IF+ LSDIR+
Sbjct: 361 QRLKDLADMLTIHYYSLFDGFDDLWHHAKSLYTETSVFSFSGKHNTGSTEIFSALSDIRN 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TL GD P EKLVHVVEKL C+  G  G+ +R  GSFIVGNQFLICGDGVQ EGLP+FKDL
Sbjct: 421 TLTGDLPSEKLVHVVEKLHCKPCGSGGVIIRAVGSFIVGNQFLICGDGVQAEGLPSFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYI 496
             D+AS+++G F+EQF++E  DLIG+YF+AKQELYI
Sbjct: 481 GFDVASRRVGRFQEQFVVESGDLIGKYFLAKQELYI 516

BLAST of Cp4.1LG03g07420 vs. ExPASy Swiss-Prot
Match: Q9FJV0 (Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK6 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 2.1e-67
Identity = 134/304 (44.08%), Postives = 190/304 (62.50%), Query Frame = 0

Query: 69  SGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINI-FEKEKRQQLLT 128
           S S E  +   + ++     IG G+  VVQ   H    +  A+K I +  ++E R+Q++ 
Sbjct: 56  SDSKELDFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQ 115

Query: 129 EIR-TLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSM 188
           E++     + C   +V +H  ++   +G  S+ LEYMD GSLADV+R  K I EP L+ +
Sbjct: 116 ELKINQASSQCPHVVVCYHSFYH---NGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVV 175

Query: 189 FQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTV 248
            +++L GL YLH  RH++HRDIKP+NLLVN KGE KI+DFG+SA L +SM    TFVGT 
Sbjct: 176 CKQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTY 235

Query: 249 TYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVN------LMLQILDDPS 308
            YMSPERI   +Y Y +DIWSLG+++ EC  G FPY  +E   N      L+  I+++P 
Sbjct: 236 NYMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPP 295

Query: 309 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 365
           P+    +FSPEFCSFV AC+QKD  AR ++  LLSHPFIKK+ED+ +DL   V  +  P+
Sbjct: 296 PTAPSDQFSPEFCSFVSACIQKDPPARASSLDLLSHPFIKKFEDKDIDLGILVGTLEPPV 355

BLAST of Cp4.1LG03g07420 vs. ExPASy Swiss-Prot
Match: A9RWC9 (Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MKK1a PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.7e-67
Identity = 136/271 (50.18%), Postives = 184/271 (67.90%), Query Frame = 0

Query: 89  IGSGASSVVQRAIHIPAHRIMALKKINI-FEKEKRQQLLTEIRTLCEAPCSEGLVEFHGA 148
           +G G+  VVQ   H   +   ALK I++  E+  R+Q++ E++    + C   ++ +H A
Sbjct: 75  LGKGSGGVVQLVRHKWTNETYALKVIHMNIEETTRKQIVQELKINHASQCPYVVICYH-A 134

Query: 149 FYTPDSGQISIALEYMDGGSLADVLR--LKKCIPEPVLSSMFQKLLQGLSYLHGMRHLVH 208
           FY  ++G ISI LEYMDGGSLAD+++    KCI EP L  + +++L GL YLH  RH++H
Sbjct: 135 FY--NNGLISIVLEYMDGGSLADIMKELEPKCIKEPNLGVVCKQVLLGLMYLHQTRHIIH 194

Query: 209 RDIKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTVTYMSPERIRNESYSYPADI 268
           RDIKP+NLLVN KGE KI+DFG+SA L NSMA+  TFVGT TYMSPER+   +Y + +DI
Sbjct: 195 RDIKPSNLLVNHKGEVKISDFGVSAVLANSMAVRDTFVGTCTYMSPERVLGGTYGFDSDI 254

Query: 269 WSLGLALFECGTGEFPY---SATEGLVN---LMLQILDDPSPSPSKHKFSPEFCSFVDAC 328
           WSLGL L EC  G++PY    + EG +N   L+  I+D P P     +FSPEFCSF+ AC
Sbjct: 255 WSLGLTLLECALGKYPYQPPGSEEGWMNFYELLQTIVDQPPPVAPADQFSPEFCSFISAC 314

Query: 329 LQKDADARPTAEQLLSHPFIKKYEDEQVDLA 351
           LQKD   RPTA +LL+HPF+ KY++E+  LA
Sbjct: 315 LQKDPKCRPTAAELLNHPFVNKYDEEEYGLA 342

BLAST of Cp4.1LG03g07420 vs. ExPASy Swiss-Prot
Match: Q5QN75 (Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MKK1 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.6e-67
Identity = 145/350 (41.43%), Postives = 211/350 (60.29%), Query Frame = 0

Query: 22  SMDSPVDP--SDSYTFSDNGTVNLLSRSYGVYNFNELGLQKCTSWLADDSGSSE---RTY 81
           + ++PVD   + S TF D           G    N+ GLQ  +   AD+  S+       
Sbjct: 16  AQETPVDKFLTASGTFKD-----------GELRLNQRGLQLISEETADEPQSTNLKVEDV 75

Query: 82  RCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINI-FEKEKRQQLLTEIRTLCEA 141
           + +  ++ +   IG G+  +VQ   H     + ALK I +  ++  R+Q++ E++ + +A
Sbjct: 76  QLSMDDLEMIQVIGKGSGGIVQLVRHKWVGTLYALKGIQMNIQEAVRKQIVQELK-INQA 135

Query: 142 PCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSMFQKLLQGLS 201
             +  +V  H +FY   +G I + LEYMD GSLAD+++  K I EP L+ + +++L+GL 
Sbjct: 136 TQNAHIVLCHQSFY--HNGVIYLVLEYMDRGSLADIIKQVKTILEPYLAVLCKQVLEGLL 195

Query: 202 YLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTVTYMSPERIR 261
           YLH  RH++HRDIKP+NLLVN KGE KITDFG+SA L +SM    TFVGT  YM+PERI 
Sbjct: 196 YLHHERHVIHRDIKPSNLLVNRKGEVKITDFGVSAVLASSMGQRDTFVGTYNYMAPERIS 255

Query: 262 NESYSYPADIWSLGLALFECGTGEFPYSATEG-----LVNLMLQILDDPSPSPSKHKFSP 321
             SY Y +DIWSLGL + EC  G FPY  +EG        L+  I+D P PS    +FSP
Sbjct: 256 GSSYDYKSDIWSLGLVILECAIGRFPYIPSEGEGWLSFYELLEAIVDQPPPSAPADQFSP 315

Query: 322 EFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 361
           EFC+F+ +C+QKD   R +A +LL+HPFIKK+ED+ +DL   V+++  P+
Sbjct: 316 EFCAFISSCIQKDPAERMSASELLNHPFIKKFEDKDLDLRILVESLEPPM 351

BLAST of Cp4.1LG03g07420 vs. ExPASy Swiss-Prot
Match: Q9S7U9 (Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK2 PE=1 SV=2)

HSP 1 Score: 243.4 bits (620), Expect = 5.3e-63
Identity = 125/286 (43.71%), Postives = 181/286 (63.29%), Query Frame = 0

Query: 89  IGSGASSVVQRAIHIPAHRIMALKKINI-FEKEKRQQLLTEIRTLCEAPCSEGLVEFHGA 148
           IG G+S VVQ   H    +  ALK I +  ++  R+ +  E++    + C   LV  + +
Sbjct: 76  IGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKINQSSQC-PNLVTSYQS 135

Query: 149 FYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSMFQKLLQGLSYLHGMRHLVHRD 208
           FY  D+G IS+ LEYMDGGSLAD L+  K IP+  LS++F+++LQGL YLH  RH++HRD
Sbjct: 136 FY--DNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIYLHHDRHIIHRD 195

Query: 209 IKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTVTYMSPERIRNESYSYPADIWS 268
           +KP+NLL+N +GE KITDFG+S  + N+  +  TFVGT  YMSPERI    Y   +DIWS
Sbjct: 196 LKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERIVGNKYGNKSDIWS 255

Query: 269 LGLALFECGTGEFPYSATE------GLVNLMLQILDDPSPSPSKHKFSPEFCSFVDACLQ 328
           LGL + EC TG+FPY+          +  LM  I+D P P+     FSPE  SF+  CLQ
Sbjct: 256 LGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNFSPELSSFISTCLQ 315

Query: 329 KDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPIQRMKDLA 368
           KD ++R +A++L+ HPF+ KY+   ++LA++  +   P+  + +L+
Sbjct: 316 KDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLATLGNLS 358

BLAST of Cp4.1LG03g07420 vs. NCBI nr
Match: XP_023528784.1 (mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 984 bits (2545), Expect = 0.0
Identity = 497/518 (95.95%), Postives = 497/518 (95.95%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI
Sbjct: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDELWHHTKALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ
Sbjct: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. NCBI nr
Match: XP_022983519.1 (mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita maxima] >XP_022983520.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita maxima] >XP_022983521.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita maxima] >XP_022983522.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita maxima])

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 492/518 (94.98%), Postives = 497/518 (95.95%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLAD+SGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADNSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFSPEFC+FVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDP+
Sbjct: 301 PSPSKHKFSPEFCTFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPV 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDELWHHTKALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TLGGDWPLEKLVHVVEKLQCRAHG+DG+AVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLGGDWPLEKLVHVVEKLQCRAHGRDGVAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ
Sbjct: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. NCBI nr
Match: KAG7017780.1 (Mitogen-activated protein kinase kinase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 976 bits (2524), Expect = 0.0
Identity = 493/518 (95.17%), Postives = 495/518 (95.56%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELKRKLTPLFDAEKGLSMDS VDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKRKLTPLFDAEKGLSMDSSVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI
Sbjct: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHESSTLSFSGKQFSG NDIFAKLSDIRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDELWHHTKALYHESSTLSFSGKQFSGANDIFAKLSDIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TLGGDWPLEKLVHVVEKLQCRAHG+DG+AVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLGGDWPLEKLVHVVEKLQCRAHGRDGVAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ
Sbjct: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. NCBI nr
Match: XP_022935524.1 (mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita moschata] >XP_022935525.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita moschata] >XP_022935526.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita moschata] >XP_022935527.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita moschata] >XP_022935528.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita moschata])

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 492/518 (94.98%), Postives = 495/518 (95.56%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELKRKLTPLFDAEKGLS+DS VDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKRKLTPLFDAEKGLSVDSSVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI
Sbjct: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHE STLSFSGKQFSGPNDIFAKLSDIRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDELWHHTKALYHERSTLSFSGKQFSGPNDIFAKLSDIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TLGGDWPLEKLVHVVEKLQCRAHG+DG+AVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLGGDWPLEKLVHVVEKLQCRAHGRDGVAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ
Sbjct: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. NCBI nr
Match: XP_004141107.1 (mitogen-activated protein kinase kinase 3 isoform X1 [Cucumis sativus] >XP_011652294.1 mitogen-activated protein kinase kinase 3 isoform X1 [Cucumis sativus] >KGN59702.1 hypothetical protein Csa_001992 [Cucumis sativus])

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 480/518 (92.66%), Postives = 492/518 (94.98%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELK+KLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKKKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLS+MFQKLL+GLSYLHG+RHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSTMFQKLLRGLSYLHGVRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFS EFCSFVDACLQKDADARPTAEQLLSHPFIKKYE+EQVDLAAFVQNVFDP 
Sbjct: 301 PSPSKHKFSSEFCSFVDACLQKDADARPTAEQLLSHPFIKKYENEQVDLAAFVQNVFDPT 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     AL+HESSTLSFSGKQFSGPNDIF KLS+IRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDDFWHHTKALFHESSTLSFSGKQFSGPNDIFGKLSEIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TL GDWPLEKLVHVVEKLQCRAHG+DG+A+RVSGSFI+GNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLAGDWPLEKLVHVVEKLQCRAHGRDGVAIRVSGSFILGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDM SKKMGSFREQFIIEPS+LIGRYFIAKQELYIIQ
Sbjct: 481 SIDMESKKMGSFREQFIIEPSNLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. ExPASy TrEMBL
Match: A0A6J1IZL9 (mitogen-activated protein kinase kinase 3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482098 PE=4 SV=1)

HSP 1 Score: 979 bits (2531), Expect = 0.0
Identity = 492/518 (94.98%), Postives = 497/518 (95.95%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLAD+SGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADNSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFSPEFC+FVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDP+
Sbjct: 301 PSPSKHKFSPEFCTFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPV 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDELWHHTKALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TLGGDWPLEKLVHVVEKLQCRAHG+DG+AVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLGGDWPLEKLVHVVEKLQCRAHGRDGVAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ
Sbjct: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. ExPASy TrEMBL
Match: A0A6J1FAS5 (mitogen-activated protein kinase kinase 3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442373 PE=4 SV=1)

HSP 1 Score: 976 bits (2523), Expect = 0.0
Identity = 492/518 (94.98%), Postives = 495/518 (95.56%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELKRKLTPLFDAEKGLS+DS VDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKRKLTPLFDAEKGLSVDSSVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI
Sbjct: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHE STLSFSGKQFSGPNDIFAKLSDIRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDELWHHTKALYHERSTLSFSGKQFSGPNDIFAKLSDIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TLGGDWPLEKLVHVVEKLQCRAHG+DG+AVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLGGDWPLEKLVHVVEKLQCRAHGRDGVAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ
Sbjct: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. ExPASy TrEMBL
Match: A0A0A0LG73 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G839800 PE=4 SV=1)

HSP 1 Score: 958 bits (2476), Expect = 0.0
Identity = 480/518 (92.66%), Postives = 492/518 (94.98%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELK+KLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKKKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLS+MFQKLL+GLSYLHG+RHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSTMFQKLLRGLSYLHGVRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           PSPSKHKFS EFCSFVDACLQKDADARPTAEQLLSHPFIKKYE+EQVDLAAFVQNVFDP 
Sbjct: 301 PSPSKHKFSSEFCSFVDACLQKDADARPTAEQLLSHPFIKKYENEQVDLAAFVQNVFDPT 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     AL+HESSTLSFSGKQFSGPNDIF KLS+IRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDDFWHHTKALFHESSTLSFSGKQFSGPNDIFGKLSEIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TL GDWPLEKLVHVVEKLQCRAHG+DG+A+RVSGSFI+GNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLAGDWPLEKLVHVVEKLQCRAHGRDGVAIRVSGSFILGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDM SKKMGSFREQFIIEPS+LIGRYFIAKQELYIIQ
Sbjct: 481 SIDMESKKMGSFREQFIIEPSNLIGRYFIAKQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. ExPASy TrEMBL
Match: A0A1S3CP64 (mitogen-activated protein kinase kinase 3 OS=Cucumis melo OX=3656 GN=LOC103502674 PE=4 SV=1)

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 480/518 (92.66%), Postives = 490/518 (94.59%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELK+KLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKKKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLS+MFQKLLQGLSYLHG+RHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSTMFQKLLQGLSYLHGVRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           P PSKHKFS EFCSFVDACLQKDADARPTAEQLLSHPFIKKYE+EQVDLAAFVQNVFDPI
Sbjct: 301 PLPSKHKFSSEFCSFVDACLQKDADARPTAEQLLSHPFIKKYENEQVDLAAFVQNVFDPI 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHESSTLSFSGKQFSGP DIF KLS+IRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDDFWHHTKALYHESSTLSFSGKQFSGPIDIFGKLSEIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TL GDWPLEKLVHVVEKLQCRAHG+DG+A+RVSGSFI+GNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLAGDWPLEKLVHVVEKLQCRAHGRDGVAIRVSGSFILGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDM SKKMGSFREQFIIEPS+LIGRYFIA QELYIIQ
Sbjct: 481 SIDMESKKMGSFREQFIIEPSNLIGRYFIANQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. ExPASy TrEMBL
Match: A0A5A7UUT4 (Mitogen-activated protein kinase kinase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00020 PE=4 SV=1)

HSP 1 Score: 955 bits (2469), Expect = 0.0
Identity = 480/518 (92.66%), Postives = 490/518 (94.59%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MAGLEELK+KLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAGLEELKKKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE
Sbjct: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE
Sbjct: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLS+MFQKLLQGLSYLHG+RHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA
Sbjct: 181 PVLSTMFQKLLQGLSYLHGVRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           P PSKHKFS EFCSFVDACLQKDADARPTAEQLLSHPFIKKYE+EQVDLAAFVQNVFDPI
Sbjct: 301 PLPSKHKFSSEFCSFVDACLQKDADARPTAEQLLSHPFIKKYENEQVDLAAFVQNVFDPI 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QRMKDLAD                     ALYHESSTLSFSGKQFSGP DIF KLS+IRS
Sbjct: 361 QRMKDLADMLTIHYYLLFDGPDDFWHHTKALYHESSTLSFSGKQFSGPIDIFGKLSEIRS 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TL GDWPLEKLVHVVEKLQCRAHG+DG+A+RVSGSFI+GNQFLICGDGVQVEGLPNFKDL
Sbjct: 421 TLAGDWPLEKLVHVVEKLQCRAHGRDGVAIRVSGSFILGNQFLICGDGVQVEGLPNFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYIIQ 497
           SIDM SKKMGSFREQFIIEPS+LIGRYFIA QELYIIQ
Sbjct: 481 SIDMESKKMGSFREQFIIEPSNLIGRYFIANQELYIIQ 518

BLAST of Cp4.1LG03g07420 vs. TAIR 10
Match: AT5G40440.1 (mitogen-activated protein kinase kinase 3 )

HSP 1 Score: 800.0 bits (2065), Expect = 1.0e-231
Identity = 393/516 (76.16%), Postives = 440/516 (85.27%), Query Frame = 0

Query: 1   MAGLEELKRKLTPLFDAEKGLSMDSPVDPSDSYTFSDNGTVNLLSRSYGVYNFNELGLQK 60
           MA LEELK+KL+PLFDAEKG S  S +DP+DSY  SD GTVNLLSRSYGVYNFNELGLQK
Sbjct: 1   MAALEELKKKLSPLFDAEKGFSSSSSLDPNDSYLLSDGGTVNLLSRSYGVYNFNELGLQK 60

Query: 61  CTSWLADDSGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKE 120
           CTS   D+S SSE TY+CAS EMR+FGAIGSGASSVVQRAIHIP HRI+ALKKINIFE+E
Sbjct: 61  CTSSHVDESESSETTYQCASHEMRVFGAIGSGASSVVQRAIHIPNHRILALKKINIFERE 120

Query: 121 KRQQLLTEIRTLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPE 180
           KRQQLLTEIRTLCEAPC EGLV+FHGAFY+PDSGQISIALEYM+GGSLAD+L++ K IPE
Sbjct: 121 KRQQLLTEIRTLCEAPCHEGLVDFHGAFYSPDSGQISIALEYMNGGSLADILKVTKKIPE 180

Query: 181 PVLSSMFQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCA 240
           PVLSS+F KLLQGLSYLHG+RHLVHRDIKPANLL+NLKGE KITDFGISAGLENSMAMCA
Sbjct: 181 PVLSSLFHKLLQGLSYLHGVRHLVHRDIKPANLLINLKGEPKITDFGISAGLENSMAMCA 240

Query: 241 TFVGTVTYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVNLMLQILDDPS 300
           TFVGTVTYMSPERIRN+SYSYPADIWSLGLALFECGTGEFPY A EG VNLMLQILDDPS
Sbjct: 241 TFVGTVTYMSPERIRNDSYSYPADIWSLGLALFECGTGEFPYIANEGPVNLMLQILDDPS 300

Query: 301 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 360
           P+P K +FSPEFCSF+DACLQKD DARPTA+QLLSHPFI K+E E+VDLA FVQ++FDP 
Sbjct: 301 PTPPKQEFSPEFCSFIDACLQKDPDARPTADQLLSHPFITKHEKERVDLATFVQSIFDPT 360

Query: 361 QRMKDLAD---------------------ALYHESSTLSFSGKQFSGPNDIFAKLSDIRS 420
           QR+KDLAD                     +LY E+S  SFSGK  +G  +IF+ LSDIR+
Sbjct: 361 QRLKDLADMLTIHYYSLFDGFDDLWHHAKSLYTETSVFSFSGKHNTGSTEIFSALSDIRN 420

Query: 421 TLGGDWPLEKLVHVVEKLQCRAHGQDGIAVRVSGSFIVGNQFLICGDGVQVEGLPNFKDL 480
           TL GD P EKLVHVVEKL C+  G  G+ +R  GSFIVGNQFLICGDGVQ EGLP+FKDL
Sbjct: 421 TLTGDLPSEKLVHVVEKLHCKPCGSGGVIIRAVGSFIVGNQFLICGDGVQAEGLPSFKDL 480

Query: 481 SIDMASKKMGSFREQFIIEPSDLIGRYFIAKQELYI 496
             D+AS+++G F+EQF++E  DLIG+YF+AKQELYI
Sbjct: 481 GFDVASRRVGRFQEQFVVESGDLIGKYFLAKQELYI 516

BLAST of Cp4.1LG03g07420 vs. TAIR 10
Match: AT5G56580.1 (MAP kinase kinase 6 )

HSP 1 Score: 258.1 bits (658), Expect = 1.5e-68
Identity = 134/304 (44.08%), Postives = 190/304 (62.50%), Query Frame = 0

Query: 69  SGSSERTYRCASREMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINI-FEKEKRQQLLT 128
           S S E  +   + ++     IG G+  VVQ   H    +  A+K I +  ++E R+Q++ 
Sbjct: 56  SDSKELDFEITAEDLETVKVIGKGSGGVVQLVRHKWVGKFFAMKVIQMNIQEEIRKQIVQ 115

Query: 129 EIR-TLCEAPCSEGLVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSM 188
           E++     + C   +V +H  ++   +G  S+ LEYMD GSLADV+R  K I EP L+ +
Sbjct: 116 ELKINQASSQCPHVVVCYHSFYH---NGAFSLVLEYMDRGSLADVIRQVKTILEPYLAVV 175

Query: 189 FQKLLQGLSYLHGMRHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTV 248
            +++L GL YLH  RH++HRDIKP+NLLVN KGE KI+DFG+SA L +SM    TFVGT 
Sbjct: 176 CKQVLLGLVYLHNERHVIHRDIKPSNLLVNHKGEVKISDFGVSASLASSMGQRDTFVGTY 235

Query: 249 TYMSPERIRNESYSYPADIWSLGLALFECGTGEFPYSATEGLVN------LMLQILDDPS 308
            YMSPERI   +Y Y +DIWSLG+++ EC  G FPY  +E   N      L+  I+++P 
Sbjct: 236 NYMSPERISGSTYDYSSDIWSLGMSVLECAIGRFPYLESEDQQNPPSFYELLAAIVENPP 295

Query: 309 PSPSKHKFSPEFCSFVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPI 365
           P+    +FSPEFCSFV AC+QKD  AR ++  LLSHPFIKK+ED+ +DL   V  +  P+
Sbjct: 296 PTAPSDQFSPEFCSFVSACIQKDPPARASSLDLLSHPFIKKFEDKDIDLGILVGTLEPPV 355

BLAST of Cp4.1LG03g07420 vs. TAIR 10
Match: AT4G29810.1 (MAP kinase kinase 2 )

HSP 1 Score: 243.4 bits (620), Expect = 3.8e-64
Identity = 125/286 (43.71%), Postives = 181/286 (63.29%), Query Frame = 0

Query: 89  IGSGASSVVQRAIHIPAHRIMALKKINI-FEKEKRQQLLTEIRTLCEAPCSEGLVEFHGA 148
           IG G+S VVQ   H    +  ALK I +  ++  R+ +  E++    + C   LV  + +
Sbjct: 76  IGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKINQSSQC-PNLVTSYQS 135

Query: 149 FYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSMFQKLLQGLSYLHGMRHLVHRD 208
           FY  D+G IS+ LEYMDGGSLAD L+  K IP+  LS++F+++LQGL YLH  RH++HRD
Sbjct: 136 FY--DNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIYLHHDRHIIHRD 195

Query: 209 IKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTVTYMSPERIRNESYSYPADIWS 268
           +KP+NLL+N +GE KITDFG+S  + N+  +  TFVGT  YMSPERI    Y   +DIWS
Sbjct: 196 LKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERIVGNKYGNKSDIWS 255

Query: 269 LGLALFECGTGEFPYSATE------GLVNLMLQILDDPSPSPSKHKFSPEFCSFVDACLQ 328
           LGL + EC TG+FPY+          +  LM  I+D P P+     FSPE  SF+  CLQ
Sbjct: 256 LGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNFSPELSSFISTCLQ 315

Query: 329 KDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPIQRMKDLA 368
           KD ++R +A++L+ HPF+ KY+   ++LA++  +   P+  + +L+
Sbjct: 316 KDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLATLGNLS 358

BLAST of Cp4.1LG03g07420 vs. TAIR 10
Match: AT4G29810.2 (MAP kinase kinase 2 )

HSP 1 Score: 243.4 bits (620), Expect = 3.8e-64
Identity = 125/286 (43.71%), Postives = 181/286 (63.29%), Query Frame = 0

Query: 89  IGSGASSVVQRAIHIPAHRIMALKKINI-FEKEKRQQLLTEIRTLCEAPCSEGLVEFHGA 148
           IG G+S VVQ   H    +  ALK I +  ++  R+ +  E++    + C   LV  + +
Sbjct: 85  IGKGSSGVVQLVQHKWTGQFFALKVIQLNIDEAIRKAIAQELKINQSSQC-PNLVTSYQS 144

Query: 149 FYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSMFQKLLQGLSYLHGMRHLVHRD 208
           FY  D+G IS+ LEYMDGGSLAD L+  K IP+  LS++F+++LQGL YLH  RH++HRD
Sbjct: 145 FY--DNGAISLILEYMDGGSLADFLKSVKAIPDSYLSAIFRQVLQGLIYLHHDRHIIHRD 204

Query: 209 IKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTVTYMSPERIRNESYSYPADIWS 268
           +KP+NLL+N +GE KITDFG+S  + N+  +  TFVGT  YMSPERI    Y   +DIWS
Sbjct: 205 LKPSNLLINHRGEVKITDFGVSTVMTNTAGLANTFVGTYNYMSPERIVGNKYGNKSDIWS 264

Query: 269 LGLALFECGTGEFPYSATE------GLVNLMLQILDDPSPSPSKHKFSPEFCSFVDACLQ 328
           LGL + EC TG+FPY+          +  LM  I+D P P+     FSPE  SF+  CLQ
Sbjct: 265 LGLVVLECATGKFPYAPPNQEETWTSVFELMEAIVDQPPPALPSGNFSPELSSFISTCLQ 324

Query: 329 KDADARPTAEQLLSHPFIKKYEDEQVDLAAFVQNVFDPIQRMKDLA 368
           KD ++R +A++L+ HPF+ KY+   ++LA++  +   P+  + +L+
Sbjct: 325 KDPNSRSSAKELMEHPFLNKYDYSGINLASYFTDAGSPLATLGNLS 367

BLAST of Cp4.1LG03g07420 vs. TAIR 10
Match: AT4G26070.2 (MAP kinase/ ERK kinase 1 )

HSP 1 Score: 228.4 bits (581), Expect = 1.3e-59
Identity = 118/278 (42.45%), Postives = 177/278 (63.67%), Query Frame = 0

Query: 82  EMRIFGAIGSGASSVVQRAIHIPAHRIMALKKINIFEKEKRQQLLT-EIRTLCEAPCSEG 141
           ++ +   IG G+S  VQ   H    +  ALK I +  +E   + ++ E+R    + C   
Sbjct: 67  DLEVIKVIGKGSSGNVQLVKHKLTQQFFALKVIQLNTEESTCRAISQELRINLSSQCPY- 126

Query: 142 LVEFHGAFYTPDSGQISIALEYMDGGSLADVLRLKKCIPEPVLSSMFQKLLQGLSYLHGM 201
           LV  + +FY   +G +SI LE+MDGGSLAD+L+    +PE +LS++ +++L+GL Y+H  
Sbjct: 127 LVSCYQSFY--HNGLVSIILEFMDGGSLADLLKKVGKVPENMLSAICKRVLRGLCYIHHE 186

Query: 202 RHLVHRDIKPANLLVNLKGEAKITDFGISAGLENSMAMCATFVGTVTYMSPERIRNESYS 261
           R ++HRD+KP+NLL+N +GE KITDFG+S  L ++ ++  +FVGT  YMSPERI    YS
Sbjct: 187 RRIIHRDLKPSNLLINHRGEVKITDFGVSKILTSTSSLANSFVGTYPYMSPERISGSLYS 246

Query: 262 YPADIWSLGLALFECGTGEFPYSATE------GLVNLMLQILDDPSPSPSKHKFSPEFCS 321
             +DIWSLGL L EC TG+FPY+  E       +  L+  I+++P P    + FSPEFCS
Sbjct: 247 NKSDIWSLGLVLLECATGKFPYTPPEHKKGWSSVYELVDAIVENPPPCAPSNLFSPEFCS 306

Query: 322 FVDACLQKDADARPTAEQLLSHPFIKKYEDEQVDLAAF 353
           F+  C+QKD   R +A++LL H F+K +ED   +L+A+
Sbjct: 307 FISQCVQKDPRDRKSAKELLEHKFVKMFEDSDTNLSAY 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O803961.5e-23076.16Mitogen-activated protein kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Q9FJV02.1e-6744.08Mitogen-activated protein kinase kinase 6 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
A9RWC92.7e-6750.18Mitogen-activated protein kinase kinase 1a OS=Physcomitrium patens OX=3218 GN=MK... [more]
Q5QN753.6e-6741.43Mitogen-activated protein kinase kinase 1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9S7U95.3e-6343.71Mitogen-activated protein kinase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=MKK... [more]
Match NameE-valueIdentityDescription
XP_023528784.10.095.95mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita pepo subsp. pepo... [more]
XP_022983519.10.094.98mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita maxima] >XP_0229... [more]
KAG7017780.10.095.17Mitogen-activated protein kinase kinase 3 [Cucurbita argyrosperma subsp. argyros... [more]
XP_022935524.10.094.98mitogen-activated protein kinase kinase 3 isoform X1 [Cucurbita moschata] >XP_02... [more]
XP_004141107.10.092.66mitogen-activated protein kinase kinase 3 isoform X1 [Cucumis sativus] >XP_01165... [more]
Match NameE-valueIdentityDescription
A0A6J1IZL90.094.98mitogen-activated protein kinase kinase 3 isoform X1 OS=Cucurbita maxima OX=3661... [more]
A0A6J1FAS50.094.98mitogen-activated protein kinase kinase 3 isoform X1 OS=Cucurbita moschata OX=36... [more]
A0A0A0LG730.092.66Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G839800 PE=4 SV=1[more]
A0A1S3CP640.092.66mitogen-activated protein kinase kinase 3 OS=Cucumis melo OX=3656 GN=LOC10350267... [more]
A0A5A7UUT40.092.66Mitogen-activated protein kinase kinase 3 OS=Cucumis melo var. makuwa OX=1194695... [more]
Match NameE-valueIdentityDescription
AT5G40440.11.0e-23176.16mitogen-activated protein kinase kinase 3 [more]
AT5G56580.11.5e-6844.08MAP kinase kinase 6 [more]
AT4G29810.13.8e-6443.71MAP kinase kinase 2 [more]
AT4G29810.23.8e-6443.71MAP kinase kinase 2 [more]
AT4G26070.21.3e-5942.45MAP kinase/ ERK kinase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 83..339
e-value: 4.1E-81
score: 285.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 87..339
e-value: 7.1E-60
score: 202.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 83..339
score: 44.654266
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 164..352
e-value: 2.3E-52
score: 179.2
NoneNo IPR availableGENE3D3.10.450.50coord: 353..496
e-value: 2.2E-10
score: 42.6
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 59..163
e-value: 1.2E-16
score: 62.5
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 167..346
e-value: 1.1E-21
score: 74.9
NoneNo IPR availablePANTHERPTHR48013:SF22SUBFAMILY NOT NAMEDcoord: 2..368
NoneNo IPR availablePANTHERPTHR48013:SF22SUBFAMILY NOT NAMEDcoord: 369..496
NoneNo IPR availablePANTHERPTHR48013DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATEDcoord: 369..496
NoneNo IPR availablePANTHERPTHR48013DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 5-RELATEDcoord: 2..368
NoneNo IPR availableCDDcd06623PKc_MAPKK_plant_likecoord: 84..343
e-value: 2.58831E-146
score: 417.763
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 203..215
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 89..112
IPR018222Nuclear transport factor 2, eukaryotePROSITEPS50177NTF2_DOMAINcoord: 348..495
score: 14.422874
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 85..369
IPR032710NTF2-like domain superfamilySUPERFAMILY54427NTF2-likecoord: 351..495

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g07420.1Cp4.1LG03g07420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000165 MAPK cascade
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004708 MAP kinase kinase activity
molecular_function GO:0004672 protein kinase activity