Cp4.1LG03g05730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g05730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein-lysine N-methyltransferase LOC111442402
LocationCp4.1LG03: 4583819 .. 4588840 (-)
RNA-Seq ExpressionCp4.1LG03g05730
SyntenyCp4.1LG03g05730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAGCCCAAATTTGACGTCTTATTACAAGCTTGACCCACCGTCCGCTGCGATCGCCTCCGTGTCGTACCAAGTTTTTTCCGGTTCAACTCCATTCCTCAGATTCCTTCTAGGGTTTTTTTTTTTTTTTCTTTTCTTCCATTTTTCCGATCATGGCTGCGATGCGATTGACGCCGGATGATGCTCGGGGTGCTCCGTCCGCCGTTGATTTGGTCTCTGACGACGATCGCTCAGTGGCCGCTGATTCTTGGTCCATCAAGAGTGAGTATGGTAGCACCCTCGACGATGACCAGCGTAATGCCGACGCTGCCGAGGCTCTATCTGCTGGAAATTTGCGTGCCGCTTCGGATTACAGGTCTAGCGTAGTTTCTTCTTTCGAACTGTGTGAATCTAACTGGAAATAGTTGAAACCGCGCTGTTTTAAAACTCTGTTTTTGTTTGACTNTTTCAGTTTTTTTTTTTTTTTTTTTTTCTTTTGGTTAGACTGATTCCTAAAGCATTCAATGATCATGTTATTCTCGTTGATTTCTGGAATAGATGAATACAGCTCCGCGGTGCAGTTCATGTAGTTTATCTTTCTCGTACACTTGAATTCCTGAAGGTGTGAGGAGAGTCCCGTTTCCCCATCTCTTGCTATTTCTTTCCTCAGTCTAATGGACTTCGTGTGGACTTCCTATACTGTTTTTCCTTTAGCTCTTCAATCTGGAGGAATGGGAGAACCTTTGTAATTGTGTCCTTTTTTATTTGCCCAGAGATTAGATCCTTGTTTCGCAACTTTTAGCTTTAACTACTGTCACAATATCAGTTGATTAGAAGCCATCATGGGTTGGCCGGACTAATGAAGAAGTAGGTTTAAAGGTAGCTACTTACTGGGATCTAATATTCTATGAGTGTCTTCAGCAACAAAATGAAGTAGAGTTTGATGGCTATCCTGCGAGATTAGTCGAGATACATATAAATAGACTTATGCACTCATGGATATCAAAGAAACCTTTGCTTAGAATTTTTGTGACCGGGAAGTTGAATATTCGATTTTTACTGCATGACCATATTAATTTGGTATAATGGTTTTGGTAATGAGCGTATTTTCATTCTAAACAAGGTTTGTTACATTTTCAGTTCTGACAAGGATGAAACAGAACCAGATGCTGAAGCTGTGACATCAATGCTAGGTCTCCAAAGTTATTGGGATTCTCAATATGCGGATGAATTGACAAACTTCCATGAACATGGTCATGTTGGTGAAGTCTGGTACGTAAGAATTGCTTAAGGTATCTTGTTTTCTTATATTCTTGTAATGTTATTATGTTTTCTGGCCTTCTGTGCTATTCAACTATAGATCATTTTCTCTAATCACTTTTGCCTGTCAATTTACTATATGTTGGTGCTGTTCTACTGTTAGTTCCATCGAGAAATTAAAGAACTATTCTTAGACTCTTAACTTTTTGAACATGATGTTGGGTATTGTTCATTCAAAGGATTATTGTACCTGATAATTCTTCTCAATAATACATCTCGAGGACTATGGTTCTTTTAAATGCAATTTGTGTTGTTAAACTGTGATCGAAAAGATAAAGTAGATTTTCTAGATTGACTGGGCAAGAATTTTTTTTGGCTAAGAAACAAGCACAACTTCATTGACGGTTAGAATCAAAGAGAGACTTGACTTGTATTTGTTCATCTAGCTTTCTATTTTTATACTTTCTTCATTCTTCAGGTTCGGACCTGAAGTTATGGAAACTGTTGCTTCTTGGACAAAAAGTTTGTGTTCTGATGTTTCGCAAGGTCGGTTTCCCAATCAAGCTGATAATGTTAAGTCACTGAATGGTGATCAGGGTTTCAAATTCTTGTCTAGTTGGAGTGTACTTGATATTGGGACGGGAAATGGTTTGCTGCTTCAAGAACTTGCTAAGGAGGGGTATGTTTCGAAATTTTGTTTTTTGATTCTTGCTAACATTTCAGAAAGGTTTTCTTTTTGACTTATATCTTATTTCAATTGGATAAACTTTTTCGTAATAGATTTACCGATTGAAGGCATAAGCTTCGGTACTTACTGTTTGTCATCTTGAACTTGCTTGCAGATTCGCTAATTTGACAGGAACTGATTATAGTGAAGGGGCAATAGATCTTGCTAGAAGCCTTGCAGAGCGAGATGGTTTTTCCAAGATTAAATTTTTGGTATGACTTGGCATGAGGATATTTTGATTACCGTACTTATTCTCTTTCAAAATCTTCACAAAGCACCTTGTCTCTCTCTTGGATGTGCTATGGAACATAGTTCTTACCTTCTACTTACAAAATTGGTGGTGTTTCTGGACTTTGGGAATTGGTAGGGATTTATTTTCGTTTTTTTGTAGAACGTGTATTTTCTTTTCCCCATGTACGTTCCTGGCTNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAATTTAAATGTTTTCTAAATGACTGTCACCATAACTATTAGGCTAATATGACTTTTGTGGCACATGTGATGTGAGTAGTGCATTGTCAATCTTGAAATTTAGGTAACCTTATTTGGCAGTTAGCCATAGGCATGAAAGGGTAAGTAATTGGGTCAATTGATCATTTTTTTTAAGGGTAAATTAGATTTTTGACTATTATTCTTTGTCTATCCTTGTGTCAATTTGATTGTTTGATCCTTGAGTTTTCAGAATTAGATCTAAATAGACTCAATATTTGTTTTTTTTCTACTTTTGTAAGGGACGACAATTGACAGCTTTGATTTGGGAGACAGATTAGGCATGTAATAGAGTTGGAGGATACCTGTTTAGTCAACTTCATAAATCCTTCCCATTGTTTGTCAGATTATGTTGCATCTAAGTTTCAAGAAATGATTGTCACGTCTGTATATTGTCAATCATTGATATCAAGCATGATTTGATGTCTAGAGATTATTTGTGAAACTTTTTAAAGTTGAGTAAATTAGTTTCCAAAAAACATATTAATAAATAGGGATAGTTGTTAAATGCCCTTGGATCGTACAAGCTGGTCATTGGTTCAATGCCTTTTTGAGCTATATAACTGGGTTATTAAATTACGGGGATCAAGCATTGTATGGTGTAGTTTGAAATTTTCAGCGTCGTGAATGCAAATCATCTGCCGATCCAGAAAGCAAGTGGAAATACAGAATTTTACACGCACAAAAGTTACAAACTCATGTCCTATCAACCTGTCTTAGAATTATTCTACTCAACCCTTTTGCTACTCCGATCTTTGGAAGTTTCTAATTCCATATGTATTAGTGGACATGTGTGCAGAAATTTTGAAGCTCAACACTTCTGTGATTCGTGAATAGTCAGGATTGGAAATATTAGTCCATTTTTTTAATTTTAAAGCTCAACATTTTATATTCAGTTTATGCATGATATAGTGCGATCATCATAATTGTACTGTTTTACATTGGCTTAGGTTGATGATGTTCTCGAGACAAAGTTGGAAGGGCAATTTCAACTCGTTGTGGATAAAGGCACTTTAGATGCAATTGGATTGCATCCAGATGGTCCCCTTAAAAGGTAACGATAAGCAAATATTTGGGCACGGCATATGCTTCATAATAATTAGATAATTGGTTGTTTTCAAGTTTCAGTTCGATGGATATTACTGCATAGAAGGTTGAAGTAATTGGACATTTGTTTTTTTTTTTTAATTATTTCCAGGATCATGTATTGGGAGTCAGTATCGAAACTGGTGGCTCCGGGTGGGCTGTTGGTAAGGGCTTTCTTTCCCCCTCCCACCGATCTGGCAGAAAAGGAAAGAATAATAATAATATATACTTCATGCTAGCGACCATGAAGTATCCTAGTGTTTTCATATCATGGGACTAAGTGATAAAGTAAAAGTAATTTAGAGTTCAAAACCTATGGCCACCCATCTAAGATTTTTAAATTCTACGAGCCTAACATCCAAATATTCATCTTTCAAAAGTTGCAATATTACCTTTCATCTCTCATAATGTTTCTAGATTACCCTTAGAGTAGAATTCCATTAAAATGTTGGACAAAAAAATTTAGACTTAGGAAGGTTGGAATGTGACTTGAAACAGAAATTGGGACCCAACACCACCACATTTGTTCTAAGTCTAAGCTTCCGTCTGAAGTCCTAATAGATATCTACACCGGTAGTTTTGAAACATGTAAAGGTGTAATATTGCAACTCCTTTGGGTCAAGGCTCTTCTTTTCCCCTTATTATATATTTCATATATCGTGAGATAAAATCAATGCGTGTGAACTCTGATTTTCCTCATTGTACATTGTATCTCCTTTGGCAATATTTCGTTCTCTTGGAGCTTGTGCATAGACATGTAGCTTCCTTTGATGAAACTGCAATGTGAACTGCCACTGTTAATTGCTGTAGGTGATCACTTCCTGCAACAGTACAAAAGATGAACTTGTGCAAGAAGTTGAGAACTTCAATCAAAGAAGGGTTAATTCCTTTGCAGAATCAGAGTCGTCCAATGGAACACAAAGAGAATCGCCTACATTTCAATACCTCAACCATGTTCGTACCTACCCGACATTCACCTTTGGAGGATCTGTGGGATCTCGCGTTGCCACAGTGGCATTTCTTAGGAACTGATTATGTGCTAGCTTGTCACCTCGGATTGAAGTCATTTTTTAATGTTAGACACATCTATGGATTTGCAGTGTTTGAGCCTACCACAGTTTGCCTCCCAATAGTCAACACTTATTATTATTTTTTTTTTTGGCTTGGAAACCACTAGACTGTGTTCTTGTTATAACTTCGAACTATTTTTCAGACTGATGTAATTACGGTGGGGAGTAAAAGTGGAGAATTGCTGAATTGCAGCTTTTGTCAGTGGGATATTGAGAATCAATGCCTTCCTCAGGATTTTAACATACAACTATGAACTATATTCCAAACTACATGAATCACCCAACCTGCCAATCACGCTATATGCGAATTTGACTTATTGATATTATTTTGCTTATGGAATCATTACTAGATTATTGTCTTTAAATATCAATGTACTAAAG

mRNA sequence

GAAAGCCCAAATTTGACGTCTTATTACAAGCTTGACCCACCGTCCGCTGCGATCGCCTCCGTGTCGTACCAAGGTTTTTTTTTTTTTTTCTTTTCTTCCATTTTTCCGATCATGGCTGCGATGCGATTGACGCCGGATGATGCTCGGGGTGCTCCGTCCGCCGTTGATTTGGTCTCTGACGACGATCGCTCAGTGGCCGCTGATTCTTGGTCCATCAAGAGTGAGTATGGTAGCACCCTCGACGATGACCAGCGTAATGCCGACGCTGCCGAGGCTCTATCTGCTGGAAATTTGCGTGCCGCTTCGGATTACAGTTCTGACAAGGATGAAACAGAACCAGATGCTGAAGCTGTGACATCAATGCTAGGTCTCCAAAGTTATTGGGATTCTCAATATGCGGATGAATTGACAAACTTCCATGAACATGGTCATGTTGGTGAAGTCTGGTTCGGACCTGAAGTTATGGAAACTGTTGCTTCTTGGACAAAAAGTCGGTTTCCCAATCAAGCTGATAATGTTAAGTCACTGAATGGTGATCAGGGTTTCAAATTCTTGTCTAGTTGGAGTGTACTTGATATTGGGACGGGAAATGGTTTGCTGCTTCAAGAACTTGCTAAGGAGGGATTCGCTAATTTGACAGGAACTGATTATAGTGAAGGGGCAATAGATCTTGCTAGAAGCCTTGCAGAGCGAGATGGTTTTTCCAAGATTAAATTTTTGGTTGATGATGTTCTCGAGACAAAGTTGGAAGGGCAATTTCAACTCGTTGTGGATAAAGGCACTTTAGATGCAATTGGATTGCATCCAGATGGTCCCCTTAAAAGGATCATGTATTGGGAGTCAGTATCGAAACTGGTGGCTCCGGGTGGGCTGTTGGTGATCACTTCCTGCAACAGTACAAAAGATGAACTTGTGCAAGAAGTTGAGAACTTCAATCAAAGAAGGGTTAATTCCTTTGCAGAATCAGAGTCGTCCAATGGAACACAAAGAGAATCGCCTACATTTCAATACCTCAACCATGTTCGTACCTACCCGACATTCACCTTTGGAGGATCTGTGGGATCTCGCGTTGCCACAGTGGCATTTCTTAGGAACTGATTATGTGCTAGCTTGTCACCTCGGATTGAAGTCATTTTTTAATGTTAGACACATCTATGGATTTGCAGTGTTTGAGCCTACCACAGTTTGCCTCCCAATAGTCAACACTTATTATTATTTTTTTTTTTGGCTTGGAAACCACTAGACTGTGTTCTTGTTATAACTTCGAACTATTTTTCAGACTGATGTAATTACGGTGGGGAGTAAAAGTGGAGAATTGCTGAATTGCAGCTTTTGTCAGTGGGATATTGAGAATCAATGCCTTCCTCAGGATTTTAACATACAACTATGAACTATATTCCAAACTACATGAATCACCCAACCTGCCAATCACGCTATATGCGAATTTGACTTATTGATATTATTTTGCTTATGGAATCATTACTAGATTATTGTCTTTAAATATCAATGTACTAAAG

Coding sequence (CDS)

GAAAGCCCAAATTTGACGTCTTATTACAAGCTTGACCCACCGTCCGCTGCGATCGCCTCCGTGTCGTACCAAGGTTTTTTTTTTTTTTTCTTTTCTTCCATTTTTCCGATCATGGCTGCGATGCGATTGACGCCGGATGATGCTCGGGGTGCTCCGTCCGCCGTTGATTTGGTCTCTGACGACGATCGCTCAGTGGCCGCTGATTCTTGGTCCATCAAGAGTGAGTATGGTAGCACCCTCGACGATGACCAGCGTAATGCCGACGCTGCCGAGGCTCTATCTGCTGGAAATTTGCGTGCCGCTTCGGATTACAGTTCTGACAAGGATGAAACAGAACCAGATGCTGAAGCTGTGACATCAATGCTAGGTCTCCAAAGTTATTGGGATTCTCAATATGCGGATGAATTGACAAACTTCCATGAACATGGTCATGTTGGTGAAGTCTGGTTCGGACCTGAAGTTATGGAAACTGTTGCTTCTTGGACAAAAAGTCGGTTTCCCAATCAAGCTGATAATGTTAAGTCACTGAATGGTGATCAGGGTTTCAAATTCTTGTCTAGTTGGAGTGTACTTGATATTGGGACGGGAAATGGTTTGCTGCTTCAAGAACTTGCTAAGGAGGGATTCGCTAATTTGACAGGAACTGATTATAGTGAAGGGGCAATAGATCTTGCTAGAAGCCTTGCAGAGCGAGATGGTTTTTCCAAGATTAAATTTTTGGTTGATGATGTTCTCGAGACAAAGTTGGAAGGGCAATTTCAACTCGTTGTGGATAAAGGCACTTTAGATGCAATTGGATTGCATCCAGATGGTCCCCTTAAAAGGATCATGTATTGGGAGTCAGTATCGAAACTGGTGGCTCCGGGTGGGCTGTTGGTGATCACTTCCTGCAACAGTACAAAAGATGAACTTGTGCAAGAAGTTGAGAACTTCAATCAAAGAAGGGTTAATTCCTTTGCAGAATCAGAGTCGTCCAATGGAACACAAAGAGAATCGCCTACATTTCAATACCTCAACCATGTTCGTACCTACCCGACATTCACCTTTGGAGGATCTGTGGGATCTCGCGTTGCCACAGTGGCATTTCTTAGGAACTGA

Protein sequence

ESPNLTSYYKLDPPSAAIASVSYQGFFFFFFSSIFPIMAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKSRFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGFANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQRESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN
Homology
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BLAST of Cp4.1LG03g05730 vs. ExPASy Swiss-Prot
Match: Q5JPI9 (EEF1A lysine methyltransferase 2 OS=Homo sapiens OX=9606 GN=EEF1AKMT2 PE=1 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-36
Identity = 94/212 (44.34%), Postives = 122/212 (57.55%), Query Frame = 0 

Query: 100 AASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVA 159
           AA    SDK     D   V S LG + +WD+ Y  EL  F E+G  GE+WFG E M  + 
Sbjct: 11  AAVAARSDKGSPGEDG-FVPSALGTREHWDAVYERELQTFREYGDTGEIWFGEESMNRLI 70

Query: 160 SW-TKSRFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGFANLTGTDYS 219
            W  K + P  A                  SVLDIGTGNG+ L ELAK GF+N+TG DYS
Sbjct: 71  RWMQKHKIPLDA------------------SVLDIGTGNGVFLVELAKFGFSNITGIDYS 130

Query: 220 EGAIDLARSLAERDGFSKIKFLVDDV--LETKLEGQFQLVVDKGTLDAIGLHPDGPL-KR 279
             AI L+ S+ E++G S IK  V+D   L T+L G F + +DKGT DAI L+PD  + KR
Sbjct: 131 PSAIQLSGSIIEKEGLSNIKLKVEDFLNLSTQLSG-FHICIDKGTFDAISLNPDNAIEKR 190

Query: 280 IMYWESVSKLVAPGGLLVITSCNSTKDELVQE 308
             Y +S+S+++   G  +ITSCN TK+EL+ E
Sbjct: 191 KQYVKSLSRVLKVKGFFLITSCNWTKEELLNE 202

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BLAST of Cp4.1LG03g05730 vs. ExPASy Swiss-Prot
Match: Q9D853 (EEF1A lysine methyltransferase 2 OS=Mus musculus OX=10090 GN=Eef1akmt2 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.1e-36
Identity = 108/281 (38.43%), Postives = 143/281 (50.89%), Query Frame = 0 

Query: 86  NADAAEALSAGNLRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHV 145
           NADA     A     + + SS  D+  P A      LG + +WD+ Y  EL  F E+G  
Sbjct: 2   NADAEGHSGAVVPAQSPEGSSAADDFVPSA------LGTREHWDAVYERELRTFQEYGDT 61

Query: 146 GEVWFGPEVMETVASW-TKSRFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQEL 205
           GE+WFG E M  +  W  K + P  A                  SVLDIGTGNG+ L EL
Sbjct: 62  GEIWFGEESMNRLIRWMQKHKIPLDA------------------SVLDIGTGNGVFLVEL 121

Query: 206 AKEGFANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVL--ETKLEGQFQLVVDKGTL 265
            K GF+N+TG DYS  AI L+ S+ E++G S I   V+D L   TKL G F + VDKGT 
Sbjct: 122 VKHGFSNITGIDYSPSAIKLSASILEKEGLSNINLKVEDFLNPSTKLSG-FHVCVDKGTY 181

Query: 266 DAIGLHPDGPL-KRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAE 325
           DAI L+PD  + KR  Y  S+S+++   G  +ITSCN TK EL           +++F+E
Sbjct: 182 DAISLNPDNAIEKRKQYVMSLSRVLEVKGFFLITSCNWTKAEL-----------LDAFSE 233

Query: 326 SESSNGTQRESPTFQYLNHVRTYPTFTFGGSVGSRVATVAF 363
                        F+    + T P F+FGG  G+ VA + F
Sbjct: 242 G------------FELFEELPT-PKFSFGGRSGNTVAALVF 233

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BLAST of Cp4.1LG03g05730 vs. ExPASy Swiss-Prot
Match: Q5D013 (EEF1A lysine methyltransferase 2 OS=Danio rerio OX=7955 GN=eef1akmt2 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 1.4e-33
Identity = 87/246 (35.37%), Postives = 128/246 (52.03%), Query Frame = 0 

Query: 120 SMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKSRFPNQADNVKSLNGD 179
           S LG + YWD  Y  EL  + + G VGE+WFG E M  V  W +++  N ++N       
Sbjct: 26  SKLGTKEYWDGAYKRELQTYKDIGDVGEIWFGEESMHRVIRWMEAQ--NISENA------ 85

Query: 180 QGFKFLSSWSVLDIGTGNGLLLQELAKEGFANLTGTDYSEGAIDLARSLAERDGFSKIKF 239
                    ++LDIGTGNG+ L ELA+ GF+NLTG DYS+ A++L  ++   +G   I  
Sbjct: 86  ---------AILDIGTGNGMFLVELARHGFSNLTGIDYSKAALELTTNILVEEGLKNINI 145

Query: 240 LVDDVL--ETKLEGQFQLVVDKGTLDAIGLHP-DGPLKRIMYWESVSKLVAPGGLLVITS 299
            V+D L   T+L+G F + +DKGT DAI L+P D    +  Y  S+  ++ P G  +ITS
Sbjct: 146 QVEDFLNPSTELKG-FDVCIDKGTFDAISLNPEDREEAKKHYVTSLRAVMRPNGFFIITS 205

Query: 300 CNSTKDELVQEVENFNQRRVNSFAESESSNGTQRESPTFQYLNHVRTYPTFTFGGSVGSR 359
           CN TK++L++  +                       P F+ +  + T P F FGG  G+ 
Sbjct: 206 CNWTKEQLLEIFK-----------------------PGFELVRELPT-PNFQFGGVTGNS 229

Query: 360 VATVAF 363
           V  + F
Sbjct: 266 VTALVF 229

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BLAST of Cp4.1LG03g05730 vs. ExPASy Swiss-Prot
Match: Q9P7Z3 (Protein-lysine N-methyltransferase efm4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=see1 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 6.5e-26
Identity = 86/255 (33.73%), Postives = 120/255 (47.06%), Query Frame = 0 

Query: 120 SMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKSRFPNQADNVKSLNGD 179
           S LG + YWD+ Y  E++NF E    GEVWFG E  E +  W +         V      
Sbjct: 7   SKLGTKQYWDNVYEREVSNFTEFNDEGEVWFGEEAEERIVQWLEDHISTSFREVSE---- 66

Query: 180 QGFKFLSSWSVLDIGTGNGLLLQELAKE------GFANLTGTDYSEGAIDLARSLAERDG 239
                 + + VLD+GTGNG LL  L +E          L G DYSE AI LA+++A    
Sbjct: 67  -----AAPFRVLDLGTGNGHLLFRLLEEEDTLLPSPCQLVGVDYSEAAIVLAKNIARHRQ 126

Query: 240 FS-KIKFLVDDVLETK--LEGQFQLVVDKGTLDAIGLHP---DGPLKRIMYWESVSKLVA 299
           FS K+KF   D+++        + L++DKGT DAI L     DG     +Y + V  +++
Sbjct: 127 FSDKVKFQQLDIIKDSKFCSKDWDLILDKGTFDAISLSGELLDGRPLNSVYVDRVRGMLS 186

Query: 300 PGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQRESPTFQYLNHVRTYPTF 359
           P G+ +ITSCN T    +QE+E           E  + NG          ++     P F
Sbjct: 187 PNGIFLITSCNWT----IQELE-----------ERFTKNGF--------IVHSTVPVPVF 229

Query: 360 TFGGSVGSRVATVAF 363
            F GS GS  + +AF
Sbjct: 247 EFQGSTGSSTSVIAF 229

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BLAST of Cp4.1LG03g05730 vs. ExPASy Swiss-Prot
Match: P40516 (Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=EFM4 PE=1 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 1.6e-24
Identity = 84/258 (32.56%), Postives = 127/258 (49.22%), Query Frame = 0 

Query: 115 AEAVTSMLGLQSYWDSQYADELTNFHEHGH-VGEVWFGPEVMETVASWTKSRFPNQADNV 174
           A+  TS LG + YWD  YA EL NF  +    G+ WF     E      +       DN+
Sbjct: 33  ADLSTSKLGTKKYWDELYALELENFRRNPQDTGDCWFSDSDAE------QKMIDFLVDNI 92

Query: 175 KSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF-ANLTGTDYSEGAIDLARSLAERD 234
            +      ++   + SV+D+GTGNG +L EL +  F   L G DYSE ++ LA ++AE  
Sbjct: 93  GA------YRISENASVVDLGTGNGHMLFELHQTEFQGKLVGIDYSEESVKLASNIAEAT 152

Query: 235 GFSK-IKFLVDDVLETKLE-GQFQLVVDKGTLDAI---GLHPDGPLKRI-MYWESVSKLV 294
           G    I F   D+     + G++ +V+DKGTLDAI   G+  +G L  + +Y   V +++
Sbjct: 153 GVDNFISFQQADIFSGDWKPGKYDIVLDKGTLDAISLSGMKINGKLDVVDVYAGVVERIL 212

Query: 295 APGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQRESPTFQYLNHVRTYPT 354
              G+ +ITSCN T+DELV+ +E  N +   +                         YP 
Sbjct: 213 KKDGIFLITSCNFTQDELVKIIETDNLKMWKTI-----------------------KYPV 255

Query: 355 FTFGGSVGSRVATVAFLR 365
           F FGG  G+ + +VAF++
Sbjct: 273 FQFGGVQGATICSVAFVK 255

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BLAST of Cp4.1LG03g05730 vs. NCBI nr
Match: KAG7017959.1 (EEF1A lysine methyltransferase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681 bits (1758), Expect = 4.40e-246
Identity = 356/382 (93.19%), Postives = 359/382 (93.98%), Query Frame = 0 

Query: 1   ESPNLTSYYKLDPPSAAIASVSYQGF------------FFFF----FSSIFPIMAAMRLT 60
           ESPNLTSYYKLDPPSA I SVSYQGF            FFFF    FSSIF IMAAMRLT
Sbjct: 1   ESPNLTSYYKLDPPSAVIGSVSYQGFSGSTPFLRFLLGFFFFSFLFFSSIFLIMAAMRLT 60

Query: 61  PDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLRAASDY 120
           PDDARGAPSAVDLVSDDDRSVAADSWSIKS+YGSTLDDDQRNADAAEALSAGNLRAASDY
Sbjct: 61  PDDARGAPSAVDLVSDDDRSVAADSWSIKSDYGSTLDDDQRNADAAEALSAGNLRAASDY 120

Query: 121 SSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKS 180
           SSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKS
Sbjct: 121 SSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKS 180

Query: 181 RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEG-FANLTGTDYSEGAID 240
           RFPNQADNVKSLNGDQG KFLSSWSVLDIGTGNGLLLQELAKEG F+NLTGTDYSEGAID
Sbjct: 181 RFPNQADNVKSLNGDQGSKFLSSWSVLDIGTGNGLLLQELAKEGGFSNLTGTDYSEGAID 240

Query: 241 LARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGPLKRIMYWESVS 300
           LARSLAERDGFS IKFLVDDVLETKLEG+FQLVVDKGTLDAIGLHPDGPLKRIMYWESVS
Sbjct: 241 LARSLAERDGFSTIKFLVDDVLETKLEGKFQLVVDKGTLDAIGLHPDGPLKRIMYWESVS 300

Query: 301 KLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQRESPTFQYLNHVRT 360
           KLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNS AESESSNGTQRESPTFQYLNHVRT
Sbjct: 301 KLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSIAESESSNGTQRESPTFQYLNHVRT 360

Query: 361 YPTFTFGGSVGSRVATVAFLRN 365
           YPTFTFGGSVGSRVATVAFLRN
Sbjct: 361 YPTFTFGGSVGSRVATVAFLRN 382

[Close]
BLAST of Cp4.1LG03g05730 vs. NCBI nr
Match: XP_023528807.1 (EEF1A lysine methyltransferase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641 bits (1653), Expect = 7.90e-231
Identity = 328/336 (97.62%), Postives = 328/336 (97.62%), Query Frame = 0 

Query: 38  MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 97
           MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN
Sbjct: 1   MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 60

Query: 98  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 157
           LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET
Sbjct: 61  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 120

Query: 158 VASWTKS--------RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF 217
           VASWTKS        RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF
Sbjct: 121 VASWTKSLCSDVSQGRFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF 180

Query: 218 ANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHP 277
           ANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHP
Sbjct: 181 ANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHP 240

Query: 278 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 337
           DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ
Sbjct: 241 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 300

Query: 338 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 365
           RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 301 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 336

[Close]
BLAST of Cp4.1LG03g05730 vs. NCBI nr
Match: KAG6581228.1 (EEF1A lysine methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 626 bits (1614), Expect = 7.19e-225
Identity = 323/337 (95.85%), Postives = 325/337 (96.44%), Query Frame = 0 

Query: 38  MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 97
           MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN
Sbjct: 1   MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 60

Query: 98  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 157
           LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET
Sbjct: 61  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 120

Query: 158 VASWTKS--------RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEG- 217
           VASWTKS        RFPNQADNVKSLNGDQG KFLSSWSVLDIGTGNGLLLQELAKEG 
Sbjct: 121 VASWTKSLCSDVSQGRFPNQADNVKSLNGDQGSKFLSSWSVLDIGTGNGLLLQELAKEGG 180

Query: 218 FANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLH 277
           F+NLTGTDYSEGAIDLARSLAERDGFS IKFLVDDVLETKLEG+FQLVVDKGTLDAIGLH
Sbjct: 181 FSNLTGTDYSEGAIDLARSLAERDGFSTIKFLVDDVLETKLEGKFQLVVDKGTLDAIGLH 240

Query: 278 PDGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGT 337
           PDGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNS AESESSNGT
Sbjct: 241 PDGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSIAESESSNGT 300

Query: 338 QRESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 365
           QRESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 301 QRESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 337

[Close]
BLAST of Cp4.1LG03g05730 vs. NCBI nr
Match: XP_022935565.1 (EEF1A lysine methyltransferase 2-like [Cucurbita moschata])

HSP 1 Score: 625 bits (1613), Expect = 9.83e-225
Identity = 320/336 (95.24%), Postives = 322/336 (95.83%), Query Frame = 0 

Query: 38  MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 97
           MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN
Sbjct: 1   MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 60

Query: 98  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 157
           LRAASDYSSDKDETEPDAEAV SMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET
Sbjct: 61  LRAASDYSSDKDETEPDAEAVASMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 120

Query: 158 VASWTK--------SRFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF 217
           VASWTK         RFPNQADNVKSLNGDQG KFLSSWSVLDIGTGNGLLLQELAKEGF
Sbjct: 121 VASWTKILCSDVSQGRFPNQADNVKSLNGDQGSKFLSSWSVLDIGTGNGLLLQELAKEGF 180

Query: 218 ANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHP 277
           +NLTGTDYSEGAIDLARSLAERDGFS IKFLVDDVLET LEGQFQLVVDKGTLDAIGLHP
Sbjct: 181 SNLTGTDYSEGAIDLARSLAERDGFSTIKFLVDDVLETNLEGQFQLVVDKGTLDAIGLHP 240

Query: 278 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 337
           DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNS AESESSNGTQ
Sbjct: 241 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSIAESESSNGTQ 300

Query: 338 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 365
           RESPTFQYLNHVRTYPTFTFGGSVGSRVATV+FLRN
Sbjct: 301 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVSFLRN 336

[Close]
BLAST of Cp4.1LG03g05730 vs. NCBI nr
Match: XP_022984107.1 (EEF1A lysine methyltransferase 2-like [Cucurbita maxima])

HSP 1 Score: 622 bits (1604), Expect = 2.31e-223
Identity = 319/336 (94.94%), Postives = 322/336 (95.83%), Query Frame = 0 

Query: 38  MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 97
           MAAMRLTPDDARGAPSAVD+VSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN
Sbjct: 1   MAAMRLTPDDARGAPSAVDMVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 60

Query: 98  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 157
            RAASDYSSDKDE EPDAE VTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET
Sbjct: 61  FRAASDYSSDKDEIEPDAENVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 120

Query: 158 VASWTKS--------RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF 217
           VASWTKS        RF NQADNVKSLNGDQG KFLSSWSVLDIGTGNGLLLQELAKEGF
Sbjct: 121 VASWTKSLCYDVSQGRFSNQADNVKSLNGDQGSKFLSSWSVLDIGTGNGLLLQELAKEGF 180

Query: 218 ANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHP 277
           +NLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLE QFQLVVDKGTLDAIGLHP
Sbjct: 181 SNLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEWQFQLVVDKGTLDAIGLHP 240

Query: 278 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 337
           +GPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ
Sbjct: 241 EGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 300

Query: 338 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 365
           RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 301 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 336

[Close]
BLAST of Cp4.1LG03g05730 vs. ExPASy TrEMBL
Match: A0A6J1FB00 (Protein-lysine N-methyltransferase LOC111442402 OS=Cucurbita moschata OX=3662 GN=LOC111442402 PE=3 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 4.76e-225
Identity = 320/336 (95.24%), Postives = 322/336 (95.83%), Query Frame = 0 

Query: 38  MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 97
           MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN
Sbjct: 1   MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 60

Query: 98  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 157
           LRAASDYSSDKDETEPDAEAV SMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET
Sbjct: 61  LRAASDYSSDKDETEPDAEAVASMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 120

Query: 158 VASWTK--------SRFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF 217
           VASWTK         RFPNQADNVKSLNGDQG KFLSSWSVLDIGTGNGLLLQELAKEGF
Sbjct: 121 VASWTKILCSDVSQGRFPNQADNVKSLNGDQGSKFLSSWSVLDIGTGNGLLLQELAKEGF 180

Query: 218 ANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHP 277
           +NLTGTDYSEGAIDLARSLAERDGFS IKFLVDDVLET LEGQFQLVVDKGTLDAIGLHP
Sbjct: 181 SNLTGTDYSEGAIDLARSLAERDGFSTIKFLVDDVLETNLEGQFQLVVDKGTLDAIGLHP 240

Query: 278 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 337
           DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNS AESESSNGTQ
Sbjct: 241 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSIAESESSNGTQ 300

Query: 338 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 365
           RESPTFQYLNHVRTYPTFTFGGSVGSRVATV+FLRN
Sbjct: 301 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVSFLRN 336

[Close]
BLAST of Cp4.1LG03g05730 vs. ExPASy TrEMBL
Match: A0A6J1J197 (Protein-lysine N-methyltransferase LOC111482514 OS=Cucurbita maxima OX=3661 GN=LOC111482514 PE=3 SV=1)

HSP 1 Score: 622 bits (1604), Expect = 1.12e-223
Identity = 319/336 (94.94%), Postives = 322/336 (95.83%), Query Frame = 0 

Query: 38  MAAMRLTPDDARGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 97
           MAAMRLTPDDARGAPSAVD+VSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN
Sbjct: 1   MAAMRLTPDDARGAPSAVDMVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGN 60

Query: 98  LRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 157
            RAASDYSSDKDE EPDAE VTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET
Sbjct: 61  FRAASDYSSDKDEIEPDAENVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMET 120

Query: 158 VASWTKS--------RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGF 217
           VASWTKS        RF NQADNVKSLNGDQG KFLSSWSVLDIGTGNGLLLQELAKEGF
Sbjct: 121 VASWTKSLCYDVSQGRFSNQADNVKSLNGDQGSKFLSSWSVLDIGTGNGLLLQELAKEGF 180

Query: 218 ANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHP 277
           +NLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLE QFQLVVDKGTLDAIGLHP
Sbjct: 181 SNLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEWQFQLVVDKGTLDAIGLHP 240

Query: 278 DGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 337
           +GPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ
Sbjct: 241 EGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQ 300

Query: 338 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 365
           RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN
Sbjct: 301 RESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 336

[Close]
BLAST of Cp4.1LG03g05730 vs. ExPASy TrEMBL
Match: A0A5D3BTP8 (Protein-lysine N-methyltransferase E5676_scaffold451G00020 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G00020 PE=3 SV=1)

HSP 1 Score: 586 bits (1510), Expect = 2.19e-208
Identity = 316/381 (82.94%), Postives = 323/381 (84.78%), Query Frame = 0 

Query: 13  PPSAAIASVSYQGFFF------------FFFSSIFPIMAAMRLTPDDA------RGAPSA 72
           PPSA I SVSYQ  FF             FF    PIMAA+RLTP+DA      R APSA
Sbjct: 17  PPSAVIGSVSYQPRFFRFNSTTQIPSRVSFFLPFLPIMAAVRLTPEDAELLQQARAAPSA 76

Query: 73  VDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLRAASDYSSDKDETEPD 132
            DLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLR ASDYSSDKDE EPD
Sbjct: 77  ADLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLRPASDYSSDKDEMEPD 136

Query: 133 AEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKS--------RF 192
           AEAVTSMLGLQSYWDSQYADELTNF EHGHVGEVWFG  VMETVASWTKS        RF
Sbjct: 137 AEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFGSGVMETVASWTKSLCYDVSQGRF 196

Query: 193 PNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGFANLTGTDYSEGAIDLAR 252
           PNQA NVK+LN DQG  FLSSW VLDIGTGNGLLLQELAKEGF+NLTGTDYSEGAIDLAR
Sbjct: 197 PNQAGNVKTLNVDQGSIFLSSWRVLDIGTGNGLLLQELAKEGFSNLTGTDYSEGAIDLAR 256

Query: 253 SLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGPLKRIMYWESVSKLV 312
           SLAERDGFS I FLVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGP+KRIMYW SVSKLV
Sbjct: 257 SLAERDGFSNINFLVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGPIKRIMYWVSVSKLV 316

Query: 313 APGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQRES--PTFQYLNHVRTY 365
           APGGLLVITSCNSTKDELVQEVENFNQRRVNSF E ESS+ TQ     PTFQYL+HVRTY
Sbjct: 317 APGGLLVITSCNSTKDELVQEVENFNQRRVNSFVEPESSDETQPGELLPTFQYLSHVRTY 376

[Close]
BLAST of Cp4.1LG03g05730 vs. ExPASy TrEMBL
Match: A0A1S3C4B8 (Protein-lysine N-methyltransferase LOC103496859 OS=Cucumis melo OX=3656 GN=LOC103496859 PE=3 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 3.30e-206
Identity = 316/384 (82.29%), Postives = 323/384 (84.11%), Query Frame = 0 

Query: 13  PPSAAIASVSYQGFFF------------FFFSSIFPIMAAMRLTPDDA------RGAPSA 72
           PPSA I SVSYQ  FF             FF    PIMAA+RLTP+DA      R APSA
Sbjct: 17  PPSAVIGSVSYQPRFFRFNSTTQIPSRVSFFLPFLPIMAAVRLTPEDAELLQQARAAPSA 76

Query: 73  VDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLRAASDYSSDKDETEPD 132
            DLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLR ASDYSSDKDE EPD
Sbjct: 77  ADLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAEALSAGNLRPASDYSSDKDEMEPD 136

Query: 133 AEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFGPEVMETVASWTKS--------RF 192
           AEAVTSMLGLQSYWDSQYADELTNF EHGHVGEVWFG  VMETVASWTKS        RF
Sbjct: 137 AEAVTSMLGLQSYWDSQYADELTNFREHGHVGEVWFGSGVMETVASWTKSLCYDVSQGRF 196

Query: 193 PNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQELAKEGFANLTGTDYSEGAIDLAR 252
           PNQA NVK+LN DQG  FLSSW VLDIGTGNGLLLQELAKEGF+NLTGTDYSEGAIDLAR
Sbjct: 197 PNQAGNVKTLNVDQGSIFLSSWRVLDIGTGNGLLLQELAKEGFSNLTGTDYSEGAIDLAR 256

Query: 253 SLAERDGFSKIKF---LVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGPLKRIMYWESVS 312
           SLAERDGFS I F   LVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGP+KRIMYW SVS
Sbjct: 257 SLAERDGFSNINFWYDLVDDVLETKLEGQFQLVVDKGTLDAIGLHPDGPIKRIMYWVSVS 316

Query: 313 KLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESESSNGTQRES--PTFQYLNHV 365
           KLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSF E ESS+ TQ     PTFQYL+HV
Sbjct: 317 KLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFVEPESSDETQPGELLPTFQYLSHV 376

[Close]
BLAST of Cp4.1LG03g05730 vs. ExPASy TrEMBL
Match: A0A6J1CQQ0 (Protein-lysine N-methyltransferase LOC111013281 OS=Momordica charantia OX=3673 GN=LOC111013281 PE=3 SV=1)

HSP 1 Score: 565 bits (1456), Expect = 4.88e-201
Identity = 293/342 (85.67%), Postives = 309/342 (90.35%), Query Frame = 0 

Query: 38  MAAMRLTPDDA------RGAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 97
           MAA+RLTP+DA      R APSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE
Sbjct: 1   MAAVRLTPEDAELLHQARAAPSAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQRNADAAE 60

Query: 98  ALSAGNLRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGHVGEVWFG 157
           ALSAGNLR ASDYSSDKDE EPDAEAVTSMLGLQSYWDSQYADEL NF EHGHVGEVWFG
Sbjct: 61  ALSAGNLRPASDYSSDKDEMEPDAEAVTSMLGLQSYWDSQYADELANFREHGHVGEVWFG 120

Query: 158 PEVMETVASWTKS--------RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTGNGLLLQE 217
            EVMETVASWTKS        + PNQ+DNVKSL+ +QG KFL+SWSVLDIGTGNGLLLQE
Sbjct: 121 SEVMETVASWTKSLCSDVSQGQIPNQSDNVKSLDVEQGSKFLTSWSVLDIGTGNGLLLQE 180

Query: 218 LAKEGFANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLVVDKGTLD 277
           LAKEGF+NLTGTDYSEGAI+LARSLAERDGFS I FLVDD+LETKL  QF+LVVDKGTLD
Sbjct: 181 LAKEGFSNLTGTDYSEGAINLARSLAERDGFSNINFLVDDILETKLGRQFELVVDKGTLD 240

Query: 278 AIGLHPDGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRRVNSFAESE 337
           AIGLHPDGP+KRIMYWESVSKLV+PGG+LVITSCNSTKDELVQEVENFNQRRVNS AE+E
Sbjct: 241 AIGLHPDGPIKRIMYWESVSKLVSPGGILVITSCNSTKDELVQEVENFNQRRVNSSAETE 300

Query: 338 SSNGTQRESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLRN 365
           S + TQRE PTFQYL HV TYPTFTFGGSVGSRVATVAFLRN
Sbjct: 301 SPSETQRELPTFQYLGHVGTYPTFTFGGSVGSRVATVAFLRN 342

[Close]
BLAST of Cp4.1LG03g05730 vs. TAIR 10
Match: AT1G66680.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.2e-120
Identity = 222/358 (62.01%), Postives = 268/358 (74.86%), Query Frame = 0 

Query: 38  MAAMRLTPDDARGAP-------------SAVDLVSDDDRSVAADSWSIKSEYGSTLDDDQ 97
           MA +RL P++    P             +   L SDDDRS+AADSWSIKSEYGSTLDDDQ
Sbjct: 1   MAGIRLLPEEPETTPQQQARAAAAVTTTTTDSLASDDDRSIAADSWSIKSEYGSTLDDDQ 60

Query: 98  RNADAAEALSAGNLRAASDYSSDKDETEPDAEAVTSMLGLQSYWDSQYADELTNFHEHGH 157
           R+ADAAEALS+ N R +SDYSSDK+E + D     SMLGLQSYWD+ Y+DELTNF EHGH
Sbjct: 61  RHADAAEALSSANFRVSSDYSSDKEEPDADGGG-QSMLGLQSYWDAAYSDELTNFREHGH 120

Query: 158 VGEVWFGPEVMETVASWTKS--------RFPNQADNVKSLNGDQGFKFLSSWSVLDIGTG 217
            GEVWFG +VME V SWTK               ++V +   DQ  K+LSSW+VLD+GTG
Sbjct: 121 AGEVWFGDDVMEIVTSWTKDLCVEISQRNMSVSENDVTTEVNDQADKYLSSWNVLDLGTG 180

Query: 218 NGLLLQELAKEGFANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLETKLEGQFQLV 277
           NGLLL +LAKEGF++LTGTDYS+GA++LA+ L++RDGF  I+F+VDD+L+TKLE QF+LV
Sbjct: 181 NGLLLHQLAKEGFSDLTGTDYSDGAVELAQHLSQRDGFPNIRFMVDDILDTKLEQQFKLV 240

Query: 278 VDKGTLDAIGLHPDGPLKRIMYWESVSKLVAPGGLLVITSCNSTKDELVQEVENFNQRR- 337
           +DKGTLDAIGLHPDGP+KR+MYW+SVSKLVAPGG+LVITSCN TKDELV+EVENFN R+ 
Sbjct: 241 MDKGTLDAIGLHPDGPVKRVMYWDSVSKLVAPGGILVITSCNHTKDELVEEVENFNIRKS 300

Query: 338 ---------VNSFAESESSNGTQRESPTFQYLNHVRTYPTFTFGGSVGSRVATVAFLR 365
                     N+   S S   ++ + P F+YL+HVRTYPTF F GSVGSRVATVAFLR
Sbjct: 301 NLCRGDGNDANNVLSSGSEAASRIDQPPFEYLSHVRTYPTFMFSGSVGSRVATVAFLR 357

[Close]
BLAST of Cp4.1LG03g05730 vs. TAIR 10
Match: AT4G34360.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.7e-08
Identity = 35/106 (33.02%), Postives = 60/106 (56.60%), Query Frame = 0 

Query: 187 SWSVLDIGTGNGLLLQELAKEGFANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVLE 246
           S SVL++G GN  L +EL K+G  ++T  D S  A++  +S     G+ +IK +  D+L+
Sbjct: 51  SSSVLELGCGNSQLCEELYKDGIVDITCIDLSSVAVEKMQSRLLPKGYKEIKVVQADMLD 110

Query: 247 TKLEGQ-FQLVVDKGTLDAIGLHPDGPLKRIMYW----ESVSKLVA 288
              + + F +V++KGT+D + +    P      W    E+VSK++A
Sbjct: 111 LPFDSESFDVVIEKGTMDVLFVDAGDP------WNPRPETVSKVMA 150

[Close]
BLAST of Cp4.1LG03g05730 vs. TAIR 10
Match: AT3G60910.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 3.7e-08
Identity = 32/118 (27.12%), Postives = 61/118 (51.69%), Query Frame = 0 

Query: 186 SSWSVLDIGTGNGLLLQELAKEGFANLTGTDYSEGAIDLARSLAERDGFSKIKFLVDDVL 245
           +S  VL +G GN L+ +++ K+G+ ++   D S  AI++ ++  +     ++K++  DV 
Sbjct: 49  TSSRVLMVGCGNSLMSEDMVKDGYEDIMNVDISSVAIEMMQT--KYASVPQLKYMQMDVR 108

Query: 246 ETKL--EGQFQLVVDKGTLDAIGLHPDGPLKRIMYWESVSKLVAPGGLLVITSCNSTK 302
           +     +  F  ++DKGTLD++    D  L        VS+L+ PGG   + +    K
Sbjct: 109 DMSYFEDDSFDTIIDKGTLDSLMCGSDALLSASRMLGEVSRLIKPGGTYFLITYGDPK 164

[Close]
BLAST of Cp4.1LG03g05730 vs. TAIR 10
Match: AT2G30920.1 (coenzyme Q 3 )

HSP 1 Score: 43.5 bits (101), Expect = 4.2e-04
Identity = 38/123 (30.89%), Postives = 61/123 (49.59%), Query Frame = 0 

Query: 180 QGFKFLSSWSVLDIGTGNGLLLQELAKEGFANLTGTDYSEGAIDLARSLAERDGF-SKIK 239
           +G KF      +DIG G GLL + LA+ G A +TG D  +  + +AR  A+ D   S I+
Sbjct: 131 EGLKF------IDIGCGGGLLSEPLARMG-ATVTGVDAVDKNVKIARLHADMDPVTSTIE 190

Query: 240 FLVDDVLETKLEG-QFQLVVDKGTLDAIGLHPDGPLKRIMYWESVSKLVAPGGLLVITSC 299
           +L     +   EG +F  V+    ++    H   P +   + +S+S L  P G  V+++ 
Sbjct: 191 YLCTTAEKLADEGRKFDAVLSLEVIE----HVANPAE---FCKSLSALTIPNGATVLSTI 239

Query: 300 NST 301
           N T
Sbjct: 251 NRT 239

The following BLAST results are available for this feature:
BLAST of Cp4.1LG03g05730 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q5JPI91.4e-3644.34EEF1A lysine methyltransferase 2 OS=Homo sapiens OX=9606 GN=EEF1AKMT2 PE=1 SV=2[more]
Q9D8533.1e-3638.43EEF1A lysine methyltransferase 2 OS=Mus musculus OX=10090 GN=Eef1akmt2 PE=1 SV=1[more]
Q5D0131.4e-3335.37EEF1A lysine methyltransferase 2 OS=Danio rerio OX=7955 GN=eef1akmt2 PE=2 SV=1[more]
Q9P7Z36.5e-2633.73Protein-lysine N-methyltransferase efm4 OS=Schizosaccharomyces pombe (strain 972... [more]
P405161.6e-2432.56Protein-lysine N-methyltransferase EFM4 OS=Saccharomyces cerevisiae (strain ATCC... [more]
BLAST of Cp4.1LG03g05730 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7017959.14.40e-24693.19EEF1A lysine methyltransferase 2, partial [Cucurbita argyrosperma subsp. argyros... [more]
XP_023528807.17.90e-23197.62EEF1A lysine methyltransferase 2-like [Cucurbita pepo subsp. pepo][more]
KAG6581228.17.19e-22595.85EEF1A lysine methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022935565.19.83e-22595.24EEF1A lysine methyltransferase 2-like [Cucurbita moschata][more]
XP_022984107.12.31e-22394.94EEF1A lysine methyltransferase 2-like [Cucurbita maxima][more]
BLAST of Cp4.1LG03g05730 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FB004.76e-22595.24Protein-lysine N-methyltransferase LOC111442402 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1J1971.12e-22394.94Protein-lysine N-methyltransferase LOC111482514 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A5D3BTP82.19e-20882.94Protein-lysine N-methyltransferase E5676_scaffold451G00020 OS=Cucumis melo var. ... [more]
A0A1S3C4B83.30e-20682.29Protein-lysine N-methyltransferase LOC103496859 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1CQQ04.88e-20185.67Protein-lysine N-methyltransferase LOC111013281 OS=Momordica charantia OX=3673 G... [more]
BLAST of Cp4.1LG03g05730 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G66680.11.2e-12062.01S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT4G34360.11.7e-0833.02S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT3G60910.13.7e-0827.12S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G30920.14.2e-0430.89coenzyme Q 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 122..338
e-value: 1.2E-32
score: 114.8
NoneNo IPR availablePANTHERPTHR12843:SF12PROTEIN-LYSINE N-METHYLTRANSFERASE 102587567coord: 38..365
NoneNo IPR availablePANTHERPTHR12843PROTEIN-LYSINE N-METHYLTRANSFERASE METTL10coord: 38..365
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 190..295
e-value: 1.95432E-14
score: 66.6846
IPR025714Methyltransferase domainPFAMPF13847Methyltransf_31coord: 189..317
e-value: 2.4E-19
score: 69.6
IPR026635Protein-lysine N-methyltransferase Efm4/METTL10HAMAPMF_03188Methyltr_EFM4coord: 114..365
score: 27.517794
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 121..311

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g05730.1Cp4.1LG03g05730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018022 peptidyl-lysine methylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016279 protein-lysine N-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity