Cp4.1LG03g05510 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g05510 vs. ExPASy Swiss-Prot
Match: Q5JK32 (Potassium transporter 5 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK5 PE=2 SV=2)

HSP 1 Score: 776.2 bits (2003), Expect = 3.6e-223
Identity = 409/787 (51.97%), Postives = 532/787 (67.60%), Query Frame = 0

Query: 26  QETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLFFSLLFSSFSVPYVHNNLKSNWQKK 85
           ++T  +   L+R DSL++E+ K+ G                            +S+  K 
Sbjct: 24  EKTLQTTTRLQRFDSLHMEAGKIPGG---------------------------QSHTAKV 83

Query: 86  KWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIFYTLTLIPVIK 145
            W+  L LAFQSIGVVYGD+GTSPLYV++  F  GIK  +D+LG MSLI YT+ L+P+IK
Sbjct: 84  GWATTLHLAFQSIGVVYGDMGTSPLYVFSSTFTNGIKDTNDILGVMSLIIYTVVLLPLIK 143

Query: 146 YVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRI 205
           Y FIVLRANDNGDGGTFALYSLI RYA++ LIPN + ED  VS+Y+L  P+ R KRA  I
Sbjct: 144 YCFIVLRANDNGDGGTFALYSLISRYARISLIPNQQAEDAMVSHYKLESPSNRVKRAHWI 203

Query: 206 QSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPAMTEGRIVWAS 265
           +  +ENS   K+ L   T+L T+MVIGDGVLTPCISVLSAVGGI E+  ++T+G+I   +
Sbjct: 204 KEKMENSPNFKIILFLVTILATSMVIGDGVLTPCISVLSAVGGIKESAKSLTQGQIAGIA 263

Query: 266 VVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYY 325
           + IL++LF+ QR GTDKVGY+F PII  WF FIA  GVYN FK+D  V+KA NP YI+ Y
Sbjct: 264 IAILIVLFLVQRFGTDKVGYSFGPIILTWFIFIAGTGVYNLFKHDTGVLKAFNPKYIVDY 323

Query: 326 FQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLICAYAGQSSFL 385
           F+RN K  WISLGGV+LCITGTEA+FAD+GHF+VR+IQ+  S+V  PS++ AY GQ+++L
Sbjct: 324 FERNGKQGWISLGGVILCITGTEAMFADLGHFNVRAIQIGFSVVLLPSVLLAYIGQAAYL 383

Query: 386 RKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVRV 445
           R + + V+ TFYKS P  LYWP FVVAV A+IIASQAMISG F+II QS  LGCFPRVRV
Sbjct: 384 RIYPEHVADTFYKSIPDPLYWPTFVVAVAAAIIASQAMISGAFAIIAQSQILGCFPRVRV 443

Query: 446 IHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMTLTSSFLVLIM 505
           IHTS K  GQVY+PEINY+LM+ CV VT +F+ T KIGNAYGIAVVFVM +T+  + L+M
Sbjct: 444 IHTSTKFHGQVYIPEINYVLMVLCVAVTAIFQTTDKIGNAYGIAVVFVMFITTLLVTLVM 503

Query: 506 VMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAIMYVWNSVFRK 565
           VMIWKT +L +  + VI G  EL+YLSS  YKF QGGYLPL F+A++M IM  W+ V   
Sbjct: 504 VMIWKTSLLWIALFPVIFGGAELIYLSSAFYKFTQGGYLPLVFSAILMFIMATWHYVHVH 563

Query: 566 KYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLV 625
           +Y YEL +K+S     E+    +  RLPG+ F Y+ELVQGIPPI+ H VE VP++ SVLV
Sbjct: 564 RYKYELRNKVSNNYVAELAVKQNLARLPGIGFLYSELVQGIPPILPHLVEKVPSIHSVLV 623

Query: 626 FVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFEKILLERLEKF 685
            ++IK LP+SK+   ERFLFR VE KE  VFRCVVRYGY +   +   FE +++E L++F
Sbjct: 624 IISIKYLPISKIETKERFLFRYVEPKEYRVFRCVVRYGYNDKVEDPAEFESLVIENLKQF 683

Query: 686 ESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQ--------------------------- 745
             +      S   +E   E +   GGV +   +                           
Sbjct: 684 IHEESLYSQSSHSLE--GESIKEIGGVTDPTSEVQDAMSSRNNSDQHTTEPRNGCMDEIQ 743

Query: 746 --------GVVHFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLK 778
                   GVVH +GET VVA+P A F  +I+++Y Y+F+++N R+ +++  +PH R+L+
Sbjct: 744 SIHKEMGNGVVHLLGETNVVAEPNADFLKKIIVDYVYNFIRKNFRQPEKITCVPHNRLLR 781

BLAST of Cp4.1LG03g05510 vs. ExPASy Swiss-Prot
Match: Q9M7K4 (Potassium transporter 5 OS=Arabidopsis thaliana OX=3702 GN=POT5 PE=1 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 4.5e-218
Identity = 403/796 (50.63%), Postives = 538/796 (67.59%), Query Frame = 0

Query: 26  QETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLFFSLLFSSFSVPYVHNNLKSNWQKK 85
           +E ++    +  H++   E +K  G+ +  D      S +  +   P  +   +S     
Sbjct: 4   EEHQIDGDEVNNHENKLNEKKKSWGKLYRPD------SFIIEAGQTP-TNTGRRS---LM 63

Query: 86  KWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIFYTLTLIPVIK 145
            W   + LAFQS+GVVYGDIGTSPLYVYA  F +GI  +DDV+G +SLI YT+TL+ ++K
Sbjct: 64  SWRTTMSLAFQSLGVVYGDIGTSPLYVYASTFTDGINDKDDVVGVLSLIIYTITLVALLK 123

Query: 146 YVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRI 205
           YVFIVL+ANDNG+GGTFALYSLICRYAK+GLIPN E ED E+SNY L LP  + +RA  I
Sbjct: 124 YVFIVLQANDNGEGGTFALYSLICRYAKMGLIPNQEPEDVELSNYTLELPTTQLRRAHMI 183

Query: 206 QSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPAMTEGRIVWAS 265
           +  LENS FAK+ L   T++GT+MVIGDG+LTP ISVLSAV GI     ++ +  +V  S
Sbjct: 184 KEKLENSKFAKIILFLVTIMGTSMVIGDGILTPSISVLSAVSGI----KSLGQNTVVGVS 243

Query: 266 VVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYY 325
           V IL++LF  QR GTDKVG++FAPII +WF F+  IG++N FK+D TV+KALNP YIIYY
Sbjct: 244 VAILIVLFAFQRFGTDKVGFSFAPIILVWFTFLIGIGLFNLFKHDITVLKALNPLYIIYY 303

Query: 326 FQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLICAYAGQSSFL 385
           F+R  +  WISLGGV LCITGTEA+FAD+GHFSVR++Q+S S V YP+L+  Y GQ+++L
Sbjct: 304 FRRTGRQGWISLGGVFLCITGTEAMFADLGHFSVRAVQISFSCVAYPALVTIYCGQAAYL 363

Query: 386 RKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVRV 445
            KH+  VS+TFY S P  LYWP FVVAV ASIIASQAMISG FS+I QSL +GCFPRV+V
Sbjct: 364 TKHTYNVSNTFYDSIPDPLYWPTFVVAVAASIIASQAMISGAFSVISQSLRMGCFPRVKV 423

Query: 446 IHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMTLTSSFLVLIM 505
           +HTSAK+EGQVY+PEINYLLMLAC+ VTL F+ T KIG+AYGIAVV VM +T+  + LIM
Sbjct: 424 VHTSAKYEGQVYIPEINYLLMLACIAVTLAFRTTEKIGHAYGIAVVTVMVITTLMVTLIM 483

Query: 506 VMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAIMYVWNSVFRK 565
           ++IWKT+I+ +  ++V+ GS+E++YLSSV+YKF  GGYLPL    V+MA+M +W  V   
Sbjct: 484 LVIWKTNIVWIAIFLVVFGSIEMLYLSSVMYKFTSGGYLPLTITVVLMAMMAIWQYVHVL 543

Query: 566 KYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLV 625
           KY YEL  KIS E   +M ++    R+PG+  FYTELV GI P+  HY+ ++ ++ SV V
Sbjct: 544 KYRYELREKISRENAIQMATSPDVNRVPGIGLFYTELVNGITPLFSHYISNLSSVHSVFV 603

Query: 626 FVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFEKILLERLEKF 685
            ++IK+LPV++V + ERF FR V  K+  +FRCVVRYGY E   E + FE+  +  L++F
Sbjct: 604 LISIKTLPVNRVTSSERFFFRYVGPKDSGMFRCVVRYGYKEDIEEPDEFERHFVYYLKEF 663

Query: 686 ESDRRALCNSDGRVEE--DEEDVNG----------------FGG---------------- 745
                 +    G V+E   EE+ N                  G                 
Sbjct: 664 IHHEHFMSGGGGEVDETDKEEEPNAETTVVPSSNYVPSSGRIGSAHSSSSDKIRSGRVVQ 723

Query: 746 ----------VAEAWKQGVVHFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVF 778
                     V +A ++G+V+ +GETE+ A+  +    + ++N+AY+FLK+N RE D+  
Sbjct: 724 VQSVEDQTELVEKAREKGMVYLMGETEITAEKESSLFKKFIVNHAYNFLKKNCREGDKAL 783

BLAST of Cp4.1LG03g05510 vs. ExPASy Swiss-Prot
Match: Q6H4M2 (Potassium transporter 19 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK19 PE=2 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 2.3e-206
Identity = 372/757 (49.14%), Postives = 512/757 (67.64%), Query Frame = 0

Query: 35  LRRHDSLYLESRKLHGRAHSEDDVGLFFSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLA 94
           LRRHDSLY ++ K+                           +N K +     W+  LQLA
Sbjct: 16  LRRHDSLYGDAEKV---------------------------SNNKRHGAGGSWARTLQLA 75

Query: 95  FQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRAN 154
           FQSIGVVYGD+GTSPLYVY+  FP GIKH DD++G +SLI YTL LIP++KYVFIVL AN
Sbjct: 76  FQSIGVVYGDVGTSPLYVYSSTFPNGIKHPDDLVGVLSLILYTLILIPMVKYVFIVLYAN 135

Query: 155 DNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRIQSALENSHF 214
           DNGDGGTFALYSLI R+AK+ +IPN + ED  VSNY +  P+ + +RA  ++  LE+S+ 
Sbjct: 136 DNGDGGTFALYSLISRHAKIRMIPNDQTEDANVSNYSIEAPSSQLRRAEWVKQKLESSNA 195

Query: 215 AKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPAMTEGRIVWASVVILVLLFM 274
           AK+ L   T+LGT+MV+GDG LTP ISVLSAV GI E  P +T+ ++VW SV IL +LF 
Sbjct: 196 AKIALFTITILGTSMVMGDGTLTPAISVLSAVSGIREKAPNLTQSQVVWISVAILFVLFS 255

Query: 275 AQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAW 334
            QR GTDKVGYTFAP+I +WF  IA IG+YN   ++ T+++A NP YI+ YF+RN K+AW
Sbjct: 256 MQRFGTDKVGYTFAPVISVWFLLIAGIGMYNLTVHEITILRAFNPKYIVDYFRRNGKEAW 315

Query: 335 ISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSH 394
           +SLGGVVLCITGTEA+FAD+GHF++R+IQLS + V +PS+   Y GQ+++LRK  + V  
Sbjct: 316 VSLGGVVLCITGTEAMFADLGHFNIRAIQLSFTCVLFPSVALCYMGQAAYLRKFPENVGD 375

Query: 395 TFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEG 454
           TFY+S P  L+WP+FVVA++ +IIASQAM+SG F+I+ ++LSLGCFPRV V+HTS K+EG
Sbjct: 376 TFYRSIPAPLFWPVFVVAIMGAIIASQAMLSGAFAILSKALSLGCFPRVEVVHTSNKYEG 435

Query: 455 QVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHIL 514
           QVY+PE+N+L+  A V VTL F+ TA IGNAYGI VV V ++T+  + ++M++IWK  + 
Sbjct: 436 QVYIPEVNFLIGAASVAVTLAFQTTANIGNAYGICVVTVFSITTHLMTVVMLLIWKVRLP 495

Query: 515 LVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHK 574
            +  +    G  E +YLSS+L KF +GGYLP  F+ V+MA+M  W+ V  K+Y+YEL+  
Sbjct: 496 FIAAFYAAFGLAEFLYLSSILSKFAEGGYLPFCFSLVLMALMATWHYVHVKRYWYELDRV 555

Query: 575 ISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPV 634
           +   +   +++    RR+PG+   Y+ELVQGIPP+    V+ +P++ +V VF++IK LPV
Sbjct: 556 VPAAETTALLARRDVRRVPGVGLLYSELVQGIPPVFPRLVDKIPSVHAVFVFMSIKHLPV 615

Query: 635 SKVPADERFLFRRVEAKEID----VFRCVVRYGYTEVRAEHELFEKILLERLEKFESDRR 694
            +V   ERF+FRRV   +      +FRCV RYGYT+     + F   LL+RL+ F  +  
Sbjct: 616 PRVAPAERFIFRRVVGADAGAGHRLFRCVARYGYTDQLEGAKEFAAFLLDRLKVFVHEES 675

Query: 695 ALCNSDGRVEED----------EEDVNGFGGVAEAWKQGVVHFIGETEVVAKPGAGFGDR 754
               S G  ++D          EE+       AE   +GVV+ +GE  V A  G+    R
Sbjct: 676 VFACSRGDNDDDDAMRRAQAMAEEEKRVIDAEAE---RGVVYLMGEANVTAAAGSSVMKR 735

Query: 755 IMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 778
           I++NY Y+ L++NLRE  +   +P  ++LKVG+TYE+
Sbjct: 736 IVVNYVYTLLRKNLREGHKALSVPKDQLLKVGITYEI 742

BLAST of Cp4.1LG03g05510 vs. ExPASy Swiss-Prot
Match: Q6H4L9 (Potassium transporter 20 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK20 PE=2 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 5.2e-206
Identity = 372/760 (48.95%), Postives = 521/760 (68.55%), Query Frame = 0

Query: 35  LRRHDSLYLESRKLHGRAHSEDDVGLFFSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLA 94
           LRRHDS Y ++ K+                           +N KS+   + W+  LQLA
Sbjct: 17  LRRHDSFYGDAEKV---------------------------SNDKSHGTGENWARTLQLA 76

Query: 95  FQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRAN 154
           FQSIGVVYGD+GTSPLYVY+  FP+G+KH DD++G +SL+ YTL LIP++KYVFIVL AN
Sbjct: 77  FQSIGVVYGDVGTSPLYVYSSTFPDGVKHPDDLVGVLSLMLYTLILIPMVKYVFIVLYAN 136

Query: 155 DNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRIQSALENSHF 214
           DNGDGGTFALYSLI R+AK+ +IPN + ED  VSNY +  P+ + +RA  ++  LE+S+ 
Sbjct: 137 DNGDGGTFALYSLISRHAKIRMIPNDQTEDANVSNYSIEAPSSQLRRAEWVKQKLESSNA 196

Query: 215 AKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPAMTEGRIVWASVVILVLLFM 274
           AK+ L   T+LGT+MV+GDG LTP ISVLSAV GI E  P++T+ ++VW SV IL++LF 
Sbjct: 197 AKIALFTITILGTSMVMGDGTLTPAISVLSAVSGIREKAPSLTQLQVVWISVPILIVLFS 256

Query: 275 AQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAW 334
            QR GTDKVGY+FAP+I +WF  IA IG YN   ++ T+++A NP YII YF+RN K+AW
Sbjct: 257 VQRFGTDKVGYSFAPVISVWFVLIAGIGAYNLAVHEITILRAFNPMYIIDYFRRNGKEAW 316

Query: 335 ISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSH 394
           +SLGG VLCITGTEA+FAD+GHF++R+IQLS + V +PS+   Y GQ+++LRK  + V  
Sbjct: 317 VSLGGAVLCITGTEAMFADLGHFNIRAIQLSFTCVLFPSVALCYMGQAAYLRKFPEDVGD 376

Query: 395 TFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEG 454
           TFYKS P  L+WP+FVVA++A+IIASQAM+SG F+I+ ++L LGCFPRV V+HTS K+EG
Sbjct: 377 TFYKSLPAPLFWPVFVVAIMAAIIASQAMLSGAFAILSKALPLGCFPRVEVVHTSNKYEG 436

Query: 455 QVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHIL 514
           QVY+PE+N+L+ +A V +T+ F+ TA IGNAYGI VV V ++T+  + ++M++IWK  + 
Sbjct: 437 QVYIPEVNFLIGVASVAITVAFQTTANIGNAYGICVVMVFSITTHLMTVVMLLIWKVRLP 496

Query: 515 LVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHK 574
            +  + V+    E +YLSS+L KF +GGYLP  F+ V+MA+M  W+ V  K+Y+YEL+H 
Sbjct: 497 FIAAFYVVFTFTEFLYLSSILSKFAEGGYLPFCFSLVLMALMATWHYVHVKRYWYELDHI 556

Query: 575 ISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPV 634
           + P++   +++    RR+PG+   YTELVQGIPP+    V+ +P++ +V VF++IK LP+
Sbjct: 557 VPPDEMAALLARRDVRRVPGVGLLYTELVQGIPPVFPRLVDKIPSVHAVFVFMSIKHLPI 616

Query: 635 SKVPADERFLFRRVEAKE-IDVFRCVVRYGYTEVRAEHELFEKILLERLEKF---ESDRR 694
            +V   ERF+F+RV       +FRCV RYGYT+     + F   LL+RL+ F   E+   
Sbjct: 617 PRVAPAERFIFQRVGPDAGHRIFRCVARYGYTDPLEGAKEFAAFLLDRLKVFVYEEAVFA 676

Query: 695 ALCNSDG-------------RVEEDEEDVNGFGGVAEAWKQGVVHFIGETEVVAKPGAGF 754
             C  DG             R EE   +       AEA ++G+V+ +GE  V A PG+  
Sbjct: 677 CQCAEDGGGGGGGDDDGVLRRAEEMAAEEKRLID-AEA-ERGLVYLMGEANVEAAPGSSL 736

Query: 755 GDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 778
             +I++NY Y+ L++NLRE  +   IP  ++LKVG+TYE+
Sbjct: 737 MKQIVVNYVYTRLRKNLREEHKALSIPKDQLLKVGITYEI 747

BLAST of Cp4.1LG03g05510 vs. ExPASy Swiss-Prot
Match: Q6VVA6 (Potassium transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=HAK1 PE=1 SV=2)

HSP 1 Score: 696.8 bits (1797), Expect = 2.7e-199
Identity = 373/823 (45.32%), Postives = 512/823 (62.21%), Query Frame = 0

Query: 14  QVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLFFSLLFSSFSVPY 73
           +V  SGS    P  T  +   L+RHDSL+ ++ K+ G  H                    
Sbjct: 6   EVEGSGSPGVEPAATATA-SRLKRHDSLFGDAEKVSGGKH-------------------- 65

Query: 74  VHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSL 133
                        W+V L LAFQS+G++YGDIGTSPLYVY+  FP+GI H DD++G +SL
Sbjct: 66  ------HGGSAVSWAVTLHLAFQSVGIIYGDIGTSPLYVYSSTFPDGIGHRDDLVGVLSL 125

Query: 134 IFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLN 193
           I YTL +IP++KYVFIVL ANDNGDGGTFALYSLI RYAK+ +IPN + ED  VSNY + 
Sbjct: 126 ILYTLIIIPMLKYVFIVLYANDNGDGGTFALYSLISRYAKIRMIPNQQAEDAMVSNYSIE 185

Query: 194 LPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEAT 253
            P+ + +RA  ++  LE+S  AK+ L F T+LGT+MV+GDG LTP ISVLSAV GI E  
Sbjct: 186 APSSQLRRAQWVKHKLESSRAAKMALFFLTILGTSMVMGDGTLTPAISVLSAVSGIREKA 245

Query: 254 PAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTV 313
           P +T+ ++V  SV IL +LF  QR GTDKVGYTFAPII +WF  IA IG+YN   ++ T+
Sbjct: 246 PNLTQTQVVLISVAILFMLFSVQRFGTDKVGYTFAPIISVWFLLIAGIGLYNLVVHEITI 305

Query: 314 IKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPS 373
           +KA NP YI+ YF+RN K  W+SLGGVVLC+TGTE +FAD+GHF++R++Q+S + + +PS
Sbjct: 306 LKAFNPWYIVQYFRRNGKKGWVSLGGVVLCVTGTEGMFADLGHFNIRAVQISFNCILFPS 365

Query: 374 LICAYAGQSSFLRKHSDLVSHTFYKSTPGS---------LYWPMFVVAVLASIIASQAMI 433
           +   Y GQ+++LRK  + VS TFYKS PG          L+WP F+VA+LA+IIASQAM+
Sbjct: 366 VALCYIGQAAYLRKFPENVSDTFYKSIPGKYRDRLNFGPLFWPTFIVAILAAIIASQAML 425

Query: 434 SGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGN 493
           SG F+I+ ++LSLGC PRVRVIHTS K+EGQVY+PE+N+++ LA + VT+ F+ T  IGN
Sbjct: 426 SGAFAILSKALSLGCLPRVRVIHTSKKYEGQVYIPEVNFMMGLASIIVTIAFRTTTSIGN 485

Query: 494 AYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYL 553
           AYGI VV    +T+  + ++M++IWK H++ ++ +  + G  E+VYLSS+L KF  GGYL
Sbjct: 486 AYGICVVTTFMVTTHLMTVVMLLIWKKHLVFILLFYCVFGFTEVVYLSSILSKFVDGGYL 545

Query: 554 PLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTT-SFRRLPGMAFFYTELV 613
           P  FA V+M +M  W+ V  ++Y+YEL+H +   +   ++      RR+PG+   YTELV
Sbjct: 546 PFCFAMVLMTMMATWHYVHVRRYWYELDHIVPTAELASLLEENGGVRRVPGVGLLYTELV 605

Query: 614 QGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYG 673
           QGIPP+    V  +P++ +V VF++IK LP+  V A ERFLFR+V  +   VFRCV RYG
Sbjct: 606 QGIPPLFPRLVRKIPSVHAVFVFISIKHLPIPHVAAAERFLFRQVGPRARRVFRCVARYG 665

Query: 674 YTEVRAEHELFEKILLERLEKF------------------------------------ES 733
           YT+   E   F   L++ L+ F                                     S
Sbjct: 666 YTDALEEPREFAAFLVDGLKMFIQEESAFAPHQEMIDAAADDDDEAAARPRRSTSSAVHS 725

Query: 734 DRRALCNSDGRVEEDEEDVNGFGGVAEAWK-------------QGVVHFIGETEVVAKPG 778
           +      S GR       +   G    A               +GVV+ +GE  V A P 
Sbjct: 726 EEAIQAASSGRTTASSVQLQAGGEPPAAMDVEEEKRLIDREVGRGVVYLMGEANVSAGPN 785

BLAST of Cp4.1LG03g05510 vs. NCBI nr
Match: XP_023526482.1 (potassium transporter 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1439 bits (3726), Expect = 0.0
Identity = 749/776 (96.52%), Postives = 750/776 (96.65%), Query Frame = 0

Query: 2   KEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLF 61
           +EVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDD    
Sbjct: 10  QEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDD---- 69

Query: 62  FSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 121
                                 KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI
Sbjct: 70  ----------------------KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 129

Query: 122 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 181
           KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE
Sbjct: 130 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 189

Query: 182 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 241
           MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS
Sbjct: 190 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 249

Query: 242 VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 301
           VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI
Sbjct: 250 VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 309

Query: 302 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 361
           GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS
Sbjct: 310 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 369

Query: 362 IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 421
           IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ
Sbjct: 370 IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 429

Query: 422 AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK 481
           AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK
Sbjct: 430 AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK 489

Query: 482 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 541
           IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG
Sbjct: 490 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 549

Query: 542 GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 601
           GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE
Sbjct: 550 GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 609

Query: 602 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 661
           LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR
Sbjct: 610 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 669

Query: 662 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV 721
           YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV
Sbjct: 670 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV 729

Query: 722 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 777
           HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL
Sbjct: 730 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 759

BLAST of Cp4.1LG03g05510 vs. NCBI nr
Match: KAG6581254.1 (Potassium transporter 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1431 bits (3704), Expect = 0.0
Identity = 732/765 (95.69%), Postives = 749/765 (97.91%), Query Frame = 0

Query: 2   KEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLF 61
           +EVSAVVAIE+E++APSGSDVPTPQE KMSWQNLRRHDSLYLESRKLHGR HSEDDVGLF
Sbjct: 10  EEVSAVVAIEEEEIAPSGSDVPTPQERKMSWQNLRRHDSLYLESRKLHGRDHSEDDVGLF 69

Query: 62  FSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 121
           F+LLFSSFSV Y++NNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI
Sbjct: 70  FTLLFSSFSVLYINNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 129

Query: 122 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 181
           KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE
Sbjct: 130 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 189

Query: 182 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 241
           +EDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKV LLFATMLGTAMVIGDGVLTPCIS
Sbjct: 190 VEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVSLLFATMLGTAMVIGDGVLTPCIS 249

Query: 242 VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 301
           VLSAVGGITEAT AMTEGRIVW SVVILV LFMAQRLGTDKVGYTFAPIIFIWFAFIAAI
Sbjct: 250 VLSAVGGITEATSAMTEGRIVWTSVVILVFLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 309

Query: 302 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 361
           GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITG EALFADVGHFSVRS
Sbjct: 310 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGAEALFADVGHFSVRS 369

Query: 362 IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 421
           IQLSMSLVTYP+LIC YAGQSSFLRK+SDLVSHTFYKSTP SLYWPMFVVAVLASIIASQ
Sbjct: 370 IQLSMSLVTYPALICVYAGQSSFLRKNSDLVSHTFYKSTPESLYWPMFVVAVLASIIASQ 429

Query: 422 AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK 481
           AMISGTFSIIQQSLSLGCFPRV+V+HTSAKHEGQVYVPEINYLLMLACVCVTLVFKNT  
Sbjct: 430 AMISGTFSIIQQSLSLGCFPRVKVVHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTTN 489

Query: 482 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 541
           IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG
Sbjct: 490 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 549

Query: 542 GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 601
           GYLPLAFAAVMM IMYVWNSVFRKKYFYELNHKISPE FNEMVSTTSFRRLPGMAFFYTE
Sbjct: 550 GYLPLAFAAVMMTIMYVWNSVFRKKYFYELNHKISPEMFNEMVSTTSFRRLPGMAFFYTE 609

Query: 602 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 661
           LVQGIPPIIKHYVEHVPALQSVLVF+TIKSLP+SKVPADERFLFRRVEAKEIDVFRCVVR
Sbjct: 610 LVQGIPPIIKHYVEHVPALQSVLVFITIKSLPISKVPADERFLFRRVEAKEIDVFRCVVR 669

Query: 662 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV 721
           YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEED NGFGG AEAWKQGVV
Sbjct: 670 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDENGFGGAAEAWKQGVV 729

Query: 722 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHK 766
           HFIGETEVVAKPGAGFGDRIMINYAY+FLKRNLR+T+QVFEIPHK
Sbjct: 730 HFIGETEVVAKPGAGFGDRIMINYAYNFLKRNLRQTNQVFEIPHK 774

BLAST of Cp4.1LG03g05510 vs. NCBI nr
Match: KAG6581255.1 (Potassium transporter 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1428 bits (3697), Expect = 0.0
Identity = 734/776 (94.59%), Postives = 751/776 (96.78%), Query Frame = 0

Query: 2   KEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLF 61
           +EVSAVV IE+E+VAPSGSDVPTPQE KMSWQNLRRHDSLYLESRKLHGR HSEDDVGLF
Sbjct: 10  EEVSAVVPIEEEEVAPSGSDVPTPQERKMSWQNLRRHDSLYLESRKLHGRDHSEDDVGLF 69

Query: 62  FSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 121
           F+LLFSSFSVPY++NNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI
Sbjct: 70  FTLLFSSFSVPYINNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 129

Query: 122 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 181
           KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE
Sbjct: 130 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 189

Query: 182 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 241
           MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKV LLFATMLGTAMVIGDG+LTPCIS
Sbjct: 190 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVSLLFATMLGTAMVIGDGILTPCIS 249

Query: 242 VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 301
           VLSAVGGITEAT AMTEGRIVW SVVILV LFM QRLGTDKVGYTFAPIIFIWFAFIAAI
Sbjct: 250 VLSAVGGITEATSAMTEGRIVWTSVVILVFLFMVQRLGTDKVGYTFAPIIFIWFAFIAAI 309

Query: 302 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 361
           GVYNFFKYDPTVIKALNPAYIIYYFQRNKK+AWISLGGVVLCITGTEALFADVGHFSVRS
Sbjct: 310 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKNAWISLGGVVLCITGTEALFADVGHFSVRS 369

Query: 362 IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 421
           IQLSMSLVTYP+LICAYAGQSSFLRKH+DLVSHTFYKSTP           VLASIIASQ
Sbjct: 370 IQLSMSLVTYPALICAYAGQSSFLRKHNDLVSHTFYKSTP-----------VLASIIASQ 429

Query: 422 AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK 481
           AMISGTFSIIQQSLSLGCFPRV+V+HTSAKHEGQVYVPEINYLLMLACVC+TLVFKNT  
Sbjct: 430 AMISGTFSIIQQSLSLGCFPRVKVVHTSAKHEGQVYVPEINYLLMLACVCITLVFKNTTN 489

Query: 482 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 541
           IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG
Sbjct: 490 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 549

Query: 542 GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 601
           GYLPLAFAAV+M IMYVWNSVFRKKYFYELNHKISPE FNEMVSTT+FRRLPGMAFFYTE
Sbjct: 550 GYLPLAFAAVLMTIMYVWNSVFRKKYFYELNHKISPEMFNEMVSTTTFRRLPGMAFFYTE 609

Query: 602 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 661
           LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRV+AKEIDVFRCVVR
Sbjct: 610 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVKAKEIDVFRCVVR 669

Query: 662 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV 721
           YGYTEVRAEHELFEKILLERLE+FESDRRALCN DGRVEEDEED NGFGGVAEAWKQGVV
Sbjct: 670 YGYTEVRAEHELFEKILLERLEEFESDRRALCNGDGRVEEDEEDGNGFGGVAEAWKQGVV 729

Query: 722 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 777
           HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL
Sbjct: 730 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 774

BLAST of Cp4.1LG03g05510 vs. NCBI nr
Match: KAG6581252.1 (Potassium transporter 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1411 bits (3653), Expect = 0.0
Identity = 731/798 (91.60%), Postives = 746/798 (93.48%), Query Frame = 0

Query: 3   EVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLFF 62
           E SAVVAIE+E+VAPSGSDVPTP E KMSWQNLRRHDSLYLESRKLHGR HSEDDVGLFF
Sbjct: 11  ESSAVVAIEEEEVAPSGSDVPTPPERKMSWQNLRRHDSLYLESRKLHGRDHSEDDVGLFF 70

Query: 63  SLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK 122
           SLLFSSFSVPY++NNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK
Sbjct: 71  SLLFSSFSVPYINNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK 130

Query: 123 HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDG----------------------- 182
           HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDG                       
Sbjct: 131 HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGKSLMRFFCDSESESELESDFDFD 190

Query: 183 -----------GTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRIQSA 242
                      GTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRIQSA
Sbjct: 191 FDFDFDFDSPGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRIQSA 250

Query: 243 LENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPAMTEGRIVWASVVI 302
           LENSHFAKV LLFATMLGTAMVIGDG+LTPCISVLSAVGGITEAT AMTEGRIVWASVVI
Sbjct: 251 LENSHFAKVSLLFATMLGTAMVIGDGILTPCISVLSAVGGITEATSAMTEGRIVWASVVI 310

Query: 303 LVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYYFQR 362
           LV LFM QRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYYFQR
Sbjct: 311 LVFLFMVQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYYFQR 370

Query: 363 NKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLICAYAGQSSFLRKH 422
           NKKDAWISLGGVVLCITG EALFADVGHFSV+SIQLSMSLVTYP+LIC YAGQSSFLRK+
Sbjct: 371 NKKDAWISLGGVVLCITGAEALFADVGHFSVQSIQLSMSLVTYPALICVYAGQSSFLRKN 430

Query: 423 SDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVRVIHT 482
           SDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRV+V+HT
Sbjct: 431 SDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVKVVHT 490

Query: 483 SAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMTLTSSFLVLIMVMI 542
           SAKHEGQVYVPEINYLLMLACVCVTLVFKNT  IGNAYGIAVVFVMTLTSSFLVLIMVMI
Sbjct: 491 SAKHEGQVYVPEINYLLMLACVCVTLVFKNTTNIGNAYGIAVVFVMTLTSSFLVLIMVMI 550

Query: 543 WKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAIMYVWNSVFRKKYF 602
           WKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMM IMYVWNSVFRKKYF
Sbjct: 551 WKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMTIMYVWNSVFRKKYF 610

Query: 603 YELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLVFVT 662
           YELNHKISPE FNEMVSTT+FRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLVFVT
Sbjct: 611 YELNHKISPEMFNEMVSTTTFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLVFVT 670

Query: 663 IKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFEKILLERLEKFESD 722
           IKSLP+SKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFEK LLERLEKFESD
Sbjct: 671 IKSLPISKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFEKNLLERLEKFESD 730

Query: 723 RRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVVHFIGETEVVAKPGAGFGDRIMINYAYS 766
           RRALCNSDGRVEEDEED NGFGG  EAWKQGVVHFIGETEVVAKPGAGFGDRIMINYAY+
Sbjct: 731 RRALCNSDGRVEEDEEDGNGFGGAVEAWKQGVVHFIGETEVVAKPGAGFGDRIMINYAYN 790

BLAST of Cp4.1LG03g05510 vs. NCBI nr
Match: XP_022935305.1 (potassium transporter 5-like [Cucurbita moschata] >XP_022935306.1 potassium transporter 5-like [Cucurbita moschata])

HSP 1 Score: 1397 bits (3617), Expect = 0.0
Identity = 725/775 (93.55%), Postives = 735/775 (94.84%), Query Frame = 0

Query: 3   EVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLFF 62
           EVSAVVAIE+E++APSGSDVPTPQE KMSWQNLRRHDSLYLESRKLHGR HSEDD     
Sbjct: 11  EVSAVVAIEEEEIAPSGSDVPTPQERKMSWQNLRRHDSLYLESRKLHGRDHSEDD----- 70

Query: 63  SLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK 122
                                KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK
Sbjct: 71  ---------------------KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK 130

Query: 123 HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEM 182
           HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEM
Sbjct: 131 HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEM 190

Query: 183 EDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISV 242
           EDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKV LLFATMLGTAMVIGDGVLTPCISV
Sbjct: 191 EDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVALLFATMLGTAMVIGDGVLTPCISV 250

Query: 243 LSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIG 302
           LSAVGGITEAT AMTEGRIVW SVVILV LFMAQRLGTDKVGYTFAPIIFIWFAFIAAIG
Sbjct: 251 LSAVGGITEATSAMTEGRIVWTSVVILVFLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIG 310

Query: 303 VYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSI 362
           VYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITG EALFADVGHFSVRSI
Sbjct: 311 VYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGAEALFADVGHFSVRSI 370

Query: 363 QLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQA 422
           QLSMSLVTYP+LIC YAGQSSFLRK+SDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQA
Sbjct: 371 QLSMSLVTYPALICVYAGQSSFLRKNSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQA 430

Query: 423 MISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKI 482
           MISGTFSIIQQSLSLGCFPRV+V+HTSAKHEGQVYVPEINYLLMLACVCVTLVFKNT  I
Sbjct: 431 MISGTFSIIQQSLSLGCFPRVKVVHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTTNI 490

Query: 483 GNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGG 542
           GNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGG
Sbjct: 491 GNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGG 550

Query: 543 YLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTEL 602
           YLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPE FNEMVSTTSFRRLPGMAFFYTEL
Sbjct: 551 YLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEMFNEMVSTTSFRRLPGMAFFYTEL 610

Query: 603 VQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRY 662
           VQGIPPIIKHYVEHVPALQSVLVFVTIKSLP+SKVPADERFLFRRVEAKEIDVFRCVVRY
Sbjct: 611 VQGIPPIIKHYVEHVPALQSVLVFVTIKSLPISKVPADERFLFRRVEAKEIDVFRCVVRY 670

Query: 663 GYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVVH 722
           GYTEVRAEHELFEKILLERLEKFESDRRALC SDGRVEEDEED NGFGG AEAWKQGVVH
Sbjct: 671 GYTEVRAEHELFEKILLERLEKFESDRRALCISDGRVEEDEEDGNGFGGAAEAWKQGVVH 730

Query: 723 FIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 777
           FIGETEVVAKPGAGFGDRIMINYAY+FLKRNLR+TDQVFEIPHKRMLKVGMTYEL
Sbjct: 731 FIGETEVVAKPGAGFGDRIMINYAYNFLKRNLRQTDQVFEIPHKRMLKVGMTYEL 759

BLAST of Cp4.1LG03g05510 vs. ExPASy TrEMBL
Match: A0A6J1F467 (Potassium transporter OS=Cucurbita moschata OX=3662 GN=LOC111442230 PE=3 SV=1)

HSP 1 Score: 1397 bits (3617), Expect = 0.0
Identity = 725/775 (93.55%), Postives = 735/775 (94.84%), Query Frame = 0

Query: 3   EVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLFF 62
           EVSAVVAIE+E++APSGSDVPTPQE KMSWQNLRRHDSLYLESRKLHGR HSEDD     
Sbjct: 11  EVSAVVAIEEEEIAPSGSDVPTPQERKMSWQNLRRHDSLYLESRKLHGRDHSEDD----- 70

Query: 63  SLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK 122
                                KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK
Sbjct: 71  ---------------------KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIK 130

Query: 123 HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEM 182
           HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEM
Sbjct: 131 HEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEM 190

Query: 183 EDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISV 242
           EDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKV LLFATMLGTAMVIGDGVLTPCISV
Sbjct: 191 EDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVALLFATMLGTAMVIGDGVLTPCISV 250

Query: 243 LSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIG 302
           LSAVGGITEAT AMTEGRIVW SVVILV LFMAQRLGTDKVGYTFAPIIFIWFAFIAAIG
Sbjct: 251 LSAVGGITEATSAMTEGRIVWTSVVILVFLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIG 310

Query: 303 VYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSI 362
           VYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITG EALFADVGHFSVRSI
Sbjct: 311 VYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGAEALFADVGHFSVRSI 370

Query: 363 QLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQA 422
           QLSMSLVTYP+LIC YAGQSSFLRK+SDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQA
Sbjct: 371 QLSMSLVTYPALICVYAGQSSFLRKNSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQA 430

Query: 423 MISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKI 482
           MISGTFSIIQQSLSLGCFPRV+V+HTSAKHEGQVYVPEINYLLMLACVCVTLVFKNT  I
Sbjct: 431 MISGTFSIIQQSLSLGCFPRVKVVHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTTNI 490

Query: 483 GNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGG 542
           GNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGG
Sbjct: 491 GNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGG 550

Query: 543 YLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTEL 602
           YLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPE FNEMVSTTSFRRLPGMAFFYTEL
Sbjct: 551 YLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEMFNEMVSTTSFRRLPGMAFFYTEL 610

Query: 603 VQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRY 662
           VQGIPPIIKHYVEHVPALQSVLVFVTIKSLP+SKVPADERFLFRRVEAKEIDVFRCVVRY
Sbjct: 611 VQGIPPIIKHYVEHVPALQSVLVFVTIKSLPISKVPADERFLFRRVEAKEIDVFRCVVRY 670

Query: 663 GYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVVH 722
           GYTEVRAEHELFEKILLERLEKFESDRRALC SDGRVEEDEED NGFGG AEAWKQGVVH
Sbjct: 671 GYTEVRAEHELFEKILLERLEKFESDRRALCISDGRVEEDEEDGNGFGGAAEAWKQGVVH 730

Query: 723 FIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 777
           FIGETEVVAKPGAGFGDRIMINYAY+FLKRNLR+TDQVFEIPHKRMLKVGMTYEL
Sbjct: 731 FIGETEVVAKPGAGFGDRIMINYAYNFLKRNLRQTDQVFEIPHKRMLKVGMTYEL 759

BLAST of Cp4.1LG03g05510 vs. ExPASy TrEMBL
Match: A0A6J1F519 (Potassium transporter OS=Cucurbita moschata OX=3662 GN=LOC111442228 PE=3 SV=1)

HSP 1 Score: 1395 bits (3611), Expect = 0.0
Identity = 722/776 (93.04%), Postives = 735/776 (94.72%), Query Frame = 0

Query: 2   KEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLF 61
           +EVSAVV IE+EQVAPSGSDVPTPQE KMSWQNLRRHDSLYLESRKLHGR HSEDD    
Sbjct: 10  EEVSAVVPIEEEQVAPSGSDVPTPQERKMSWQNLRRHDSLYLESRKLHGRDHSEDD---- 69

Query: 62  FSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 121
                                 KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI
Sbjct: 70  ----------------------KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 129

Query: 122 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 181
           KHEDDVLGAMSLIFYTLTL+PVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE
Sbjct: 130 KHEDDVLGAMSLIFYTLTLLPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 189

Query: 182 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 241
           MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKV LLFATMLGTAMVIGDGVLTPCIS
Sbjct: 190 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVSLLFATMLGTAMVIGDGVLTPCIS 249

Query: 242 VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 301
           VLSAVGGITEAT AMTEGRIVWASVVILV LFM QRLGTDKVGYTFAPIIFIWFAFIA I
Sbjct: 250 VLSAVGGITEATSAMTEGRIVWASVVILVFLFMVQRLGTDKVGYTFAPIIFIWFAFIATI 309

Query: 302 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 361
           GVYNFFKYDPTVIKALNPAYIIYYFQRNKK+AWISLGGVVLCITGTEALFADVGHFSVRS
Sbjct: 310 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKNAWISLGGVVLCITGTEALFADVGHFSVRS 369

Query: 362 IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 421
           IQLSMSLVTYP+LICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ
Sbjct: 370 IQLSMSLVTYPALICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 429

Query: 422 AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK 481
           AMISGTFSIIQQSLSLGCFPRV+V+HTSAKHEGQVYVPEINYLLMLACVC+TLVFKNT  
Sbjct: 430 AMISGTFSIIQQSLSLGCFPRVKVVHTSAKHEGQVYVPEINYLLMLACVCITLVFKNTTN 489

Query: 482 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 541
           IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG
Sbjct: 490 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 549

Query: 542 GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 601
           GYLPLAFAAV+M IMYVWNSVFRKKYFYELNHKISPE FNEMVSTTSFRRLPGMAFFYTE
Sbjct: 550 GYLPLAFAAVLMTIMYVWNSVFRKKYFYELNHKISPEMFNEMVSTTSFRRLPGMAFFYTE 609

Query: 602 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 661
           LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRV+AKEIDVFRCVVR
Sbjct: 610 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVKAKEIDVFRCVVR 669

Query: 662 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV 721
           YGYTEVRAEHELFEKILLERLE+FE DRRALCN DGRVEEDEED NGFGGVA+AWKQGVV
Sbjct: 670 YGYTEVRAEHELFEKILLERLEEFEGDRRALCNDDGRVEEDEEDGNGFGGVAKAWKQGVV 729

Query: 722 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 777
           HFIGETEVVAKPGAGFGDRIMINYAY+FLKRNLRETDQVFEIPHKRMLKVGMTYEL
Sbjct: 730 HFIGETEVVAKPGAGFGDRIMINYAYTFLKRNLRETDQVFEIPHKRMLKVGMTYEL 759

BLAST of Cp4.1LG03g05510 vs. ExPASy TrEMBL
Match: A0A6J1J7D5 (Potassium transporter OS=Cucurbita maxima OX=3661 GN=LOC111482046 PE=3 SV=1)

HSP 1 Score: 1389 bits (3595), Expect = 0.0
Identity = 720/776 (92.78%), Postives = 734/776 (94.59%), Query Frame = 0

Query: 2   KEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLF 61
           +EVSAVV+IE+E+VAPSGSDVPTPQE KMSWQNLRRHDSLYLESRKLHGR HS+ D    
Sbjct: 10  QEVSAVVSIEEEEVAPSGSDVPTPQERKMSWQNLRRHDSLYLESRKLHGRDHSDAD---- 69

Query: 62  FSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 121
                                 KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFP+GI
Sbjct: 70  ----------------------KKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPKGI 129

Query: 122 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 181
           KH+DDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE
Sbjct: 130 KHQDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 189

Query: 182 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 241
           MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS
Sbjct: 190 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 249

Query: 242 VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 301
           VLSAVGGIT ATPAMTEGRIVW SV ILV LFMAQRLGTDKVGYTFAPIIFIWFAFIAAI
Sbjct: 250 VLSAVGGITAATPAMTEGRIVWTSVAILVFLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 309

Query: 302 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 361
           GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS
Sbjct: 310 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 369

Query: 362 IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 421
           IQLSMSLVTYP+LICAYAGQSSFLRKHSDLVS+ FYKSTPGSLYWPMFVVAVLASIIASQ
Sbjct: 370 IQLSMSLVTYPALICAYAGQSSFLRKHSDLVSNAFYKSTPGSLYWPMFVVAVLASIIASQ 429

Query: 422 AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK 481
           AMISGTFSIIQQSLSLGCFPRV+V+HTSAKHEGQVYVPEINYLLMLACVCVTLVFKNT  
Sbjct: 430 AMISGTFSIIQQSLSLGCFPRVKVVHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTTN 489

Query: 482 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 541
           IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG
Sbjct: 490 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 549

Query: 542 GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 601
           GYLPLAFAAV+M IMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE
Sbjct: 550 GYLPLAFAAVLMTIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 609

Query: 602 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 661
           LVQGIPPIIKHY+EHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR
Sbjct: 610 LVQGIPPIIKHYIEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 669

Query: 662 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV 721
           YGYTEVRAEHELFEKILLERLE+FESD RALCN DGRVEE EED NGFGGVAEAWKQGVV
Sbjct: 670 YGYTEVRAEHELFEKILLERLEEFESDGRALCNGDGRVEEAEEDRNGFGGVAEAWKQGVV 729

Query: 722 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 777
           HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHK MLKVGMTYEL
Sbjct: 730 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKCMLKVGMTYEL 759

BLAST of Cp4.1LG03g05510 vs. ExPASy TrEMBL
Match: A0A6J1HLM3 (Potassium transporter OS=Cucurbita moschata OX=3662 GN=LOC111464714 PE=3 SV=1)

HSP 1 Score: 1378 bits (3567), Expect = 0.0
Identity = 716/776 (92.27%), Postives = 735/776 (94.72%), Query Frame = 0

Query: 2   KEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLF 61
           +EVSAVVAIE+E+VAPSGSDVPTPQE KMSWQNLRRHDSLYLESRKLHGR HS+ D    
Sbjct: 10  EEVSAVVAIEEEEVAPSGSDVPTPQERKMSWQNLRRHDSLYLESRKLHGRDHSDAD---- 69

Query: 62  FSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGI 121
                                 KKKWSVILQLAF SIGVVYGDIGTSPLYVYAGIFPEGI
Sbjct: 70  ----------------------KKKWSVILQLAFLSIGVVYGDIGTSPLYVYAGIFPEGI 129

Query: 122 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAE 181
           KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAK+GLIPNAE
Sbjct: 130 KHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKLGLIPNAE 189

Query: 182 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCIS 241
           MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKV LLFATMLGTAMVIGDGVLTPCIS
Sbjct: 190 MEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVSLLFATMLGTAMVIGDGVLTPCIS 249

Query: 242 VLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAI 301
           VLSAVGGITEATPAMTEGRIVWASVVILV LFMAQRLGT+KVGYTFAPIIFIWFAFIAAI
Sbjct: 250 VLSAVGGITEATPAMTEGRIVWASVVILVFLFMAQRLGTNKVGYTFAPIIFIWFAFIAAI 309

Query: 302 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRS 361
           GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITG EALFADVGHFS++S
Sbjct: 310 GVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGAEALFADVGHFSIQS 369

Query: 362 IQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQ 421
           IQLSMSLVTYP+LICAYAGQSSFLRKHSDLVS+TFYKSTP SLYWPMFVVAVLASIIASQ
Sbjct: 370 IQLSMSLVTYPALICAYAGQSSFLRKHSDLVSNTFYKSTPESLYWPMFVVAVLASIIASQ 429

Query: 422 AMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAK 481
           AMISGTFSIIQQSLSLGCFPRV+V+HTSAKHEGQVYVPEINYLLMLACVC+TLVFKNT K
Sbjct: 430 AMISGTFSIIQQSLSLGCFPRVKVVHTSAKHEGQVYVPEINYLLMLACVCITLVFKNTTK 489

Query: 482 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 541
           IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG
Sbjct: 490 IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQG 549

Query: 542 GYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTE 601
           GYLPLAFAAV+M IMYVWNSVFRKKYFYEL+HKISPEKFN+MVSTTSFRRLPGMAFFYTE
Sbjct: 550 GYLPLAFAAVLMIIMYVWNSVFRKKYFYELSHKISPEKFNQMVSTTSFRRLPGMAFFYTE 609

Query: 602 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 661
           LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR
Sbjct: 610 LVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVR 669

Query: 662 YGYTEVRAEHELFEKILLERLEKFESDRRALCNSDGRVEEDEEDVNGFGGVAEAWKQGVV 721
           YGYTEVRAEHELFEKILL+RLE+FESDRRALCN DGRVEE EED N FGGVAEAWKQGVV
Sbjct: 670 YGYTEVRAEHELFEKILLKRLEEFESDRRALCNCDGRVEEVEEDGNEFGGVAEAWKQGVV 729

Query: 722 HFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKRMLKVGMTYEL 777
           HFIGETEVVAK GAGFGDRIMINYAYSFL+RNLRETDQVFEIPHKRMLKVGMTYEL
Sbjct: 730 HFIGETEVVAKLGAGFGDRIMINYAYSFLRRNLRETDQVFEIPHKRMLKVGMTYEL 759

BLAST of Cp4.1LG03g05510 vs. ExPASy TrEMBL
Match: A0A1S4E0I1 (Potassium transporter OS=Cucumis melo OX=3656 GN=LOC103494897 PE=3 SV=1)

HSP 1 Score: 1139 bits (2947), Expect = 0.0
Identity = 605/790 (76.58%), Postives = 662/790 (83.80%), Query Frame = 0

Query: 1   MKEVSAVVAIEQEQVAPSGSDVPTPQETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGL 60
           M    A+V  E E+V    S   +  E K SWQ LRR+DSL LESRKLHG  H +D    
Sbjct: 1   MSSSDAIVKEEDEEVVVGPSS--STSERKPSWQKLRRYDSLDLESRKLHG--HDDD---- 60

Query: 61  FFSLLFSSFSVPYVHNNLKSNWQKKKWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEG 120
                         H+  K N     WSVIL LAFQSIG+VYGDIGTSPLYV++  FP G
Sbjct: 61  --------------HSYAKEN----NWSVILHLAFQSIGIVYGDIGTSPLYVFSSTFPGG 120

Query: 121 IKHEDDVLGAMSLIFYTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNA 180
           IKH DD+LG +SLI YT+TLIPVIKYVFIVL+ANDNG+GGTFALYSLICRYAKVGLIPNA
Sbjct: 121 IKHNDDILGVLSLIIYTITLIPVIKYVFIVLKANDNGEGGTFALYSLICRYAKVGLIPNA 180

Query: 181 EMEDGEVSNYQLNLPNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCI 240
           E+ED EVSNYQL+LPN R+KRASRIQS LE SHFAKVFLLFATMLGT+MVIGDGVLTPCI
Sbjct: 181 EVEDREVSNYQLSLPNEREKRASRIQSKLEKSHFAKVFLLFATMLGTSMVIGDGVLTPCI 240

Query: 241 SVLSAVGGITEATPAMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAA 300
           SVLSA+GGI EATPAMTE RIVWASV ILV LFM QR GTDKVGYTFAPIIF+WFA  A+
Sbjct: 241 SVLSAMGGIKEATPAMTEERIVWASVGILVCLFMVQRFGTDKVGYTFAPIIFVWFALNAS 300

Query: 301 IGVYNFFKYDPTVIKALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVR 360
           IGVYNF KYDP V+KALNP YII +FQRNK DAWISLGGVVL ITGTEALFADVGHFSVR
Sbjct: 301 IGVYNFIKYDPAVLKALNPNYIIQFFQRNKMDAWISLGGVVLAITGTEALFADVGHFSVR 360

Query: 361 SIQLSMSLVTYPSLICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIAS 420
           SIQLSMS +TYP+LICAY GQ+SFLRKH+DLVS TFYKS PG LYWPMFVVAV ASIIAS
Sbjct: 361 SIQLSMSAITYPALICAYVGQASFLRKHNDLVSDTFYKSIPGGLYWPMFVVAVSASIIAS 420

Query: 421 QAMISGTFSIIQQSLSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTA 480
           QAMISGTFSIIQQSLSLGCFPRV+V+HTS K+EGQVYVPEINYLLMLACV VTL FKNT 
Sbjct: 421 QAMISGTFSIIQQSLSLGCFPRVKVVHTSDKYEGQVYVPEINYLLMLACVGVTLGFKNTT 480

Query: 481 KIGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQ 540
           +IGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHIL +ITY++ IG+VELVYLSSVLYKFDQ
Sbjct: 481 QIGNAYGIAVVFVMTLTSSFLVLIMVMIWKTHILFIITYILTIGTVELVYLSSVLYKFDQ 540

Query: 541 GGYLPLAFAAVMMAIMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYT 600
           GGYLPLAFAA +M IMYVWNSVFRKKYFYELNHKI+ EK NE+VSTT+FRR+PG+AFFY+
Sbjct: 541 GGYLPLAFAAALMTIMYVWNSVFRKKYFYELNHKITSEKLNEIVSTTNFRRIPGIAFFYS 600

Query: 601 ELVQGIPPIIKHYVEHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVV 660
           ELVQGIPPI KHYV+HVPALQSVL+F+TIKSLPVSKVP DERFLFR+VEAKEIDVFRCVV
Sbjct: 601 ELVQGIPPIFKHYVDHVPALQSVLIFITIKSLPVSKVPVDERFLFRKVEAKEIDVFRCVV 660

Query: 661 RYGYTEVRAEHELFEKILLERLEKFESDR-RALCNS-----DGRVEEDE-------EDVN 720
           RYGYT+VR EHE FEKILLERLE+FE +R RA         DGRVE+DE       ++  
Sbjct: 661 RYGYTDVRTEHESFEKILLERLEEFERERVRADSKEENGVLDGRVEKDESCNEEEEDNYK 720

Query: 721 GFGGVAEAWKQGVVHFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVFEIPHKR 777
             G + EA K GVVH +GE+EVVAK GA FG RIMINYAYSFLKRNLR++DQVF IP  R
Sbjct: 721 AIGRIEEARKDGVVHLVGESEVVAKKGASFGKRIMINYAYSFLKRNLRQSDQVFGIPRMR 764

BLAST of Cp4.1LG03g05510 vs. TAIR 10
Match: AT4G13420.1 (high affinity K+ transporter 5 )

HSP 1 Score: 759.2 bits (1959), Expect = 3.2e-219
Identity = 403/796 (50.63%), Postives = 538/796 (67.59%), Query Frame = 0

Query: 26  QETKMSWQNLRRHDSLYLESRKLHGRAHSEDDVGLFFSLLFSSFSVPYVHNNLKSNWQKK 85
           +E ++    +  H++   E +K  G+ +  D      S +  +   P  +   +S     
Sbjct: 4   EEHQIDGDEVNNHENKLNEKKKSWGKLYRPD------SFIIEAGQTP-TNTGRRS---LM 63

Query: 86  KWSVILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIFYTLTLIPVIK 145
            W   + LAFQS+GVVYGDIGTSPLYVYA  F +GI  +DDV+G +SLI YT+TL+ ++K
Sbjct: 64  SWRTTMSLAFQSLGVVYGDIGTSPLYVYASTFTDGINDKDDVVGVLSLIIYTITLVALLK 123

Query: 146 YVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRI 205
           YVFIVL+ANDNG+GGTFALYSLICRYAK+GLIPN E ED E+SNY L LP  + +RA  I
Sbjct: 124 YVFIVLQANDNGEGGTFALYSLICRYAKMGLIPNQEPEDVELSNYTLELPTTQLRRAHMI 183

Query: 206 QSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPAMTEGRIVWAS 265
           +  LENS FAK+ L   T++GT+MVIGDG+LTP ISVLSAV GI     ++ +  +V  S
Sbjct: 184 KEKLENSKFAKIILFLVTIMGTSMVIGDGILTPSISVLSAVSGI----KSLGQNTVVGVS 243

Query: 266 VVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYY 325
           V IL++LF  QR GTDKVG++FAPII +WF F+  IG++N FK+D TV+KALNP YIIYY
Sbjct: 244 VAILIVLFAFQRFGTDKVGFSFAPIILVWFTFLIGIGLFNLFKHDITVLKALNPLYIIYY 303

Query: 326 FQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLICAYAGQSSFL 385
           F+R  +  WISLGGV LCITGTEA+FAD+GHFSVR++Q+S S V YP+L+  Y GQ+++L
Sbjct: 304 FRRTGRQGWISLGGVFLCITGTEAMFADLGHFSVRAVQISFSCVAYPALVTIYCGQAAYL 363

Query: 386 RKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVRV 445
            KH+  VS+TFY S P  LYWP FVVAV ASIIASQAMISG FS+I QSL +GCFPRV+V
Sbjct: 364 TKHTYNVSNTFYDSIPDPLYWPTFVVAVAASIIASQAMISGAFSVISQSLRMGCFPRVKV 423

Query: 446 IHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMTLTSSFLVLIM 505
           +HTSAK+EGQVY+PEINYLLMLAC+ VTL F+ T KIG+AYGIAVV VM +T+  + LIM
Sbjct: 424 VHTSAKYEGQVYIPEINYLLMLACIAVTLAFRTTEKIGHAYGIAVVTVMVITTLMVTLIM 483

Query: 506 VMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAIMYVWNSVFRK 565
           ++IWKT+I+ +  ++V+ GS+E++YLSSV+YKF  GGYLPL    V+MA+M +W  V   
Sbjct: 484 LVIWKTNIVWIAIFLVVFGSIEMLYLSSVMYKFTSGGYLPLTITVVLMAMMAIWQYVHVL 543

Query: 566 KYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLV 625
           KY YEL  KIS E   +M ++    R+PG+  FYTELV GI P+  HY+ ++ ++ SV V
Sbjct: 544 KYRYELREKISRENAIQMATSPDVNRVPGIGLFYTELVNGITPLFSHYISNLSSVHSVFV 603

Query: 626 FVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFEKILLERLEKF 685
            ++IK+LPV++V + ERF FR V  K+  +FRCVVRYGY E   E + FE+  +  L++F
Sbjct: 604 LISIKTLPVNRVTSSERFFFRYVGPKDSGMFRCVVRYGYKEDIEEPDEFERHFVYYLKEF 663

Query: 686 ESDRRALCNSDGRVEE--DEEDVNG----------------FGG---------------- 745
                 +    G V+E   EE+ N                  G                 
Sbjct: 664 IHHEHFMSGGGGEVDETDKEEEPNAETTVVPSSNYVPSSGRIGSAHSSSSDKIRSGRVVQ 723

Query: 746 ----------VAEAWKQGVVHFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETDQVF 778
                     V +A ++G+V+ +GETE+ A+  +    + ++N+AY+FLK+N RE D+  
Sbjct: 724 VQSVEDQTELVEKAREKGMVYLMGETEITAEKESSLFKKFIVNHAYNFLKKNCREGDKAL 783

BLAST of Cp4.1LG03g05510 vs. TAIR 10
Match: AT1G60160.1 (Potassium transporter family protein )

HSP 1 Score: 641.0 bits (1652), Expect = 1.3e-183
Identity = 337/737 (45.73%), Postives = 478/737 (64.86%), Query Frame = 0

Query: 91  LQLAFQSIGVVYGDIGTSPLYVYAGIFPE-GIKHEDDVLGAMSLIFYTLTLIPVIKYVFI 150
           L +AFQ++GVVYGD+GTSPLYV++ +F +  I+ E DVLGA+SL+ YT+ +IP+ KYVF+
Sbjct: 89  LGIAFQTLGVVYGDMGTSPLYVFSDVFSKVPIRSEVDVLGALSLVIYTIAVIPLAKYVFV 148

Query: 151 VLRANDNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLPNRRQKRASRIQSAL 210
           VL+ANDNG+GGTFALYSLICRYAKV  +PN +  D ++S+++L LP    +RA  I+ AL
Sbjct: 149 VLKANDNGEGGTFALYSLICRYAKVNKLPNQQPADEQISSFRLKLPTPELERALGIKEAL 208

Query: 211 ENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPAMTEGRIVWASVVIL 270
           E   + K  LL   ++GT+M+IGDG+LTP +SV+SA+ G+           +V +S+VIL
Sbjct: 209 ETKGYLKTLLLLLVLMGTSMIIGDGILTPAMSVMSAMSGLQGEVKGFGTNALVMSSIVIL 268

Query: 271 VLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIKALNPAYIIYYFQRN 330
           V LF  QR GT KVG+ FAP++ +WF  + AIG+YN  KYD TVI+ALNP YI+ +F +N
Sbjct: 269 VALFSIQRFGTGKVGFLFAPVLALWFFSLGAIGIYNLLKYDFTVIRALNPFYIVLFFNKN 328

Query: 331 KKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLICAYAGQSSFLRKHS 390
            K AW +LGG VLCITG EA+FAD+GHFSVRSIQ++ + V +P L+ AY GQ+++L KH 
Sbjct: 329 SKQAWSALGGCVLCITGAEAMFADLGHFSVRSIQMAFTCVVFPCLLLAYMGQAAYLTKHP 388

Query: 391 DLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSLSLGCFPRVRVIHTS 450
           +  +  FY S P SL+WP+FV+A LA++IASQAMIS TFS ++Q+++LGCFPR+++IHTS
Sbjct: 389 EASARIFYDSVPKSLFWPVFVIATLAAMIASQAMISATFSCVKQAMALGCFPRLKIIHTS 448

Query: 451 AKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMTLTSSFLVLIMVMIW 510
            K  GQ+Y+P IN+ LM+ C+ V  +F++T  I NAYGIA V VM +++  + L+M++IW
Sbjct: 449 KKRIGQIYIPVINWFLMIMCILVVSIFRSTTHIANAYGIAEVGVMMVSTVLVTLVMLLIW 508

Query: 511 KTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAIMYVWNSVFRKKYFY 570
           +T+I L + + +I GSVE +YL +VL K  +GG++PL FA   + +MY+WN     KY  
Sbjct: 509 QTNIFLALCFPLIFGSVETIYLLAVLTKILEGGWVPLVFATFFLTVMYIWNYGSVLKYQS 568

Query: 571 ELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVEHVPALQSVLVFVTI 630
           E+  +IS +   E+ ST    R+PG+   Y ELVQGIP I   ++  +PA+ S ++FV I
Sbjct: 569 EVRERISMDFMRELGSTLGTIRIPGIGLLYNELVQGIPSIFGQFLLTLPAIHSTIIFVCI 628

Query: 631 KSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAE-HELFEKILLERLEKF--- 690
           K +PV  VP +ERFLFRRV  K+  +FRC+ RYGY +VR E   +FE++L+E LEKF   
Sbjct: 629 KYVPVPVVPQEERFLFRRVCPKDYHMFRCIARYGYKDVRKEDSRVFEQLLIESLEKFLRC 688

Query: 691 ---------------------------------------------ESDRRALCNSD--GR 750
                                                        E D   L +S     
Sbjct: 689 EALEDALESTLNDFDPDRVSVASDTYTDDLMAPLIHRAKRSEPEQELDSEVLPSSSVGSS 748

Query: 751 VEEDEEDVNGFGGVAEAWKQGVVHFIGETEVVAKPGAGFGDRIMINYAYSFLKRNLRETD 776
           +EED         + EA   G+ + +   +V AK  + F  +++INY Y+FL+RN R   
Sbjct: 749 MEEDPALEYELAALREATDSGLTYLLAHGDVRAKKNSIFVKKLVINYFYAFLRRNCRAGA 808

BLAST of Cp4.1LG03g05510 vs. TAIR 10
Match: AT1G31120.1 (K+ uptake permease 10 )

HSP 1 Score: 638.3 bits (1645), Expect = 8.2e-183
Identity = 339/754 (44.96%), Postives = 486/754 (64.46%), Query Frame = 0

Query: 78  LKSNWQKKKWS--VILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIF 137
           L++ +++KK+S  ++LQL+FQS+GVVYGD+GTSPLYV+   FP GIK  +D++GA+SLI 
Sbjct: 42  LRNMYREKKFSAFLLLQLSFQSLGVVYGDLGTSPLYVFYNTFPRGIKDPEDIIGALSLII 101

Query: 138 YTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLP 197
           Y+LTLIP++KYVF+V +ANDNG GGTFALYSL+CR+AKV  IPN    D E++ Y     
Sbjct: 102 YSLTLIPLLKYVFVVCKANDNGQGGTFALYSLLCRHAKVSTIPNQHRTDEELTTYSRTTF 161

Query: 198 NRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPA 257
           + R   A++ +  LEN    K  LL   ++GT MVIGDG+LTP ISVLSA GG+    P 
Sbjct: 162 HER-SFAAKTKRWLENGTSRKNALLILVLVGTCMVIGDGILTPAISVLSAAGGLRVNLPH 221

Query: 258 MTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIK 317
           +  G +V  +VVILV LF  Q  GTD+VG+ FAPI+F+WF FIA+IG++N +K+DP+V+K
Sbjct: 222 INNGIVVVVAVVILVSLFSVQHYGTDRVGWLFAPIVFLWFLFIASIGMFNIWKHDPSVLK 281

Query: 318 ALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLI 377
           A +P YI  YF+R  +D W SLGG++L ITG EALFAD+ HF V ++Q + +++ +P L+
Sbjct: 282 AFSPVYIFRYFKRGGQDRWTSLGGIMLSITGIEALFADLSHFPVSAVQFAFTVIVFPCLL 341

Query: 378 CAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSL 437
            AY+GQ+++LRK+   V   FY+S P  +YWPMF++A  A+I+ASQA IS TFS+I+Q+L
Sbjct: 342 LAYSGQAAYLRKYPHHVEDAFYQSIPKRVYWPMFIIATAAAIVASQATISATFSLIKQAL 401

Query: 438 SLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMT 497
           + GCFPRV+V+HTS K  GQ+YVP+IN++LM+ C+ VT  FKN  +IGNAYG AVV VM 
Sbjct: 402 AHGCFPRVKVVHTSRKFLGQIYVPDINWILMILCIAVTAGFKNQNQIGNAYGTAVVIVML 461

Query: 498 LTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAI 557
           +T+  ++LIM+++W+ H +LV+ + ++   VE  Y S+VL+K +QGG++PL  AA  + I
Sbjct: 462 VTTLLMMLIMILVWRCHWVLVLLFTLLSLVVECTYFSAVLFKVNQGGWVPLVIAAAFLVI 521

Query: 558 MYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVE 617
           MYVW+    K+Y +E++ K+S      +  +    R+PG+   YTEL  G+P I  H++ 
Sbjct: 522 MYVWHYGTLKRYEFEMHSKVSMAWILGLGPSLGLVRVPGIGLVYTELASGVPHIFSHFIT 581

Query: 618 HVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFE 677
           ++PA  SV++FV +K+LPV  VP +ERFL +R+  K   +FRCV RYGY ++  + + FE
Sbjct: 582 NLPATHSVVIFVCVKNLPVYTVPQEERFLVKRIGPKNFHMFRCVARYGYRDLHKKDDDFE 641

Query: 678 KILLERLEKF-----------ESDRRALCNSDGRVEEDEEDVNG---------------- 737
           K L E L  F           +S+  ++C S  R   D  + NG                
Sbjct: 642 KRLFESLFLFLRLESMMEGCSDSEDYSVCGSQQRQSRDGVNGNGNEIRNVSTFDTFDSIE 701

Query: 738 ---------------------FGGVAE------AWKQGVVHFIGETEVVAKPGAGFGDRI 776
                                 GG  E          GVVH +G T V A+  A F  RI
Sbjct: 702 SVIAPTTTKRTSHTVTGSSQMSGGGDEVEFINGCRDAGVVHIMGNTVVRARREARFYKRI 761

BLAST of Cp4.1LG03g05510 vs. TAIR 10
Match: AT2G35060.1 (K+ uptake permease 11 )

HSP 1 Score: 632.9 bits (1631), Expect = 3.5e-181
Identity = 333/749 (44.46%), Postives = 493/749 (65.82%), Query Frame = 0

Query: 78  LKSNWQKKKWS--VILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIF 137
           L++ +++KK+S  ++LQL+FQS+GVVYGD+GTSPLYV+   FP GIK  +D++GA+SLI 
Sbjct: 43  LRNMYREKKFSALLLLQLSFQSLGVVYGDLGTSPLYVFYNTFPHGIKDPEDIIGALSLII 102

Query: 138 YTLTLIPVIKYVFIVLRANDNGDGGTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNLP 197
           Y+LTLIP++KYVF+V +ANDNG GGTFALYSL+CR+AKV  I N    D E++ Y     
Sbjct: 103 YSLTLIPLLKYVFVVCKANDNGQGGTFALYSLLCRHAKVKTIQNQHRTDEELTTYS-RTT 162

Query: 198 NRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATPA 257
                 A++ +  LE     K  LL   ++GT MVIGDG+LTP ISVLSA GG+    P 
Sbjct: 163 FHEHSFAAKTKRWLEKRTSRKTALLILVLVGTCMVIGDGILTPAISVLSAAGGLRVNLPH 222

Query: 258 MTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVIK 317
           ++ G +V+ +VVILV LF  Q  GTD+VG+ FAPI+F+WF  IA+IG+YN +K+D +V+K
Sbjct: 223 ISNGVVVFVAVVILVSLFSVQHYGTDRVGWLFAPIVFLWFLSIASIGMYNIWKHDTSVLK 282

Query: 318 ALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSLI 377
           A +P YI  YF+R  +D W SLGG++L ITG EALFAD+ HF V ++Q++ +++ +P L+
Sbjct: 283 AFSPVYIYRYFKRGGRDRWTSLGGIMLSITGIEALFADLSHFPVSAVQIAFTVIVFPCLL 342

Query: 378 CAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQSL 437
            AY+GQ++++R++ D V+  FY+S PGS+YWPMF++A  A+I+ASQA IS TFS+++Q+L
Sbjct: 343 LAYSGQAAYIRRYPDHVADAFYRSIPGSVYWPMFIIATAAAIVASQATISATFSLVKQAL 402

Query: 438 SLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVMT 497
           + GCFPRV+V+HTS K  GQ+YVP+IN++LM+ C+ VT  FKN ++IGNAYG AVV VM 
Sbjct: 403 AHGCFPRVKVVHTSRKFLGQIYVPDINWILMILCIAVTAGFKNQSQIGNAYGTAVVIVML 462

Query: 498 LTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMAI 557
           +T+  + LIM+++W+ H +LV+ + V+   VE  Y S++L+K DQGG++PL  AA  + I
Sbjct: 463 VTTLLMTLIMILVWRCHWVLVLIFTVLSLVVECTYFSAMLFKIDQGGWVPLVIAAAFLLI 522

Query: 558 MYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYVE 617
           M+VW+    K+Y +E++ ++S      +  +    R+PG+   YTEL  G+P I  H++ 
Sbjct: 523 MWVWHYGTLKRYEFEMHCRVSMAWILGLGPSLGLVRVPGVGLVYTELASGVPHIFSHFIT 582

Query: 618 HVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELFE 677
           ++PA+ SV+VFV +K+LPV  VP +ERFL +R+  K   +FRCV RYGY ++  + + FE
Sbjct: 583 NLPAIHSVVVFVCVKNLPVYTVPEEERFLVKRIGPKNFHMFRCVARYGYRDLHKKDDDFE 642

Query: 678 KILLE------RLEKF------ESDRRALCNSDGRVEE------DEEDVNGF-------- 737
           K L E      RLE        +SD  ++C S  ++++      + E++  F        
Sbjct: 643 KRLFESLFLYVRLESMMEGGCSDSDDYSICGSQQQLKDTLGNGNENENLATFDTFDSIES 702

Query: 738 -----------------GGVAE------AWKQGVVHFIGETEVVAKPGAGFGDRIMINYA 776
                             GV E          GVVH +G T V A+  A F  +I I+Y 
Sbjct: 703 ITPVKRVSNTVTASSQMSGVDELEFINGCRDAGVVHIMGNTVVRARREARFYKKIAIDYV 762

BLAST of Cp4.1LG03g05510 vs. TAIR 10
Match: AT2G35060.2 (K+ uptake permease 11 )

HSP 1 Score: 628.2 bits (1619), Expect = 8.5e-180
Identity = 333/750 (44.40%), Postives = 493/750 (65.73%), Query Frame = 0

Query: 78  LKSNWQKKKWS--VILQLAFQSIGVVYGDIGTSPLYVYAGIFPEGIKHEDDVLGAMSLIF 137
           L++ +++KK+S  ++LQL+FQS+GVVYGD+GTSPLYV+   FP GIK  +D++GA+SLI 
Sbjct: 43  LRNMYREKKFSALLLLQLSFQSLGVVYGDLGTSPLYVFYNTFPHGIKDPEDIIGALSLII 102

Query: 138 YTLTLIPVIKYVFIVLRANDNGDG-GTFALYSLICRYAKVGLIPNAEMEDGEVSNYQLNL 197
           Y+LTLIP++KYVF+V +ANDNG G GTFALYSL+CR+AKV  I N    D E++ Y    
Sbjct: 103 YSLTLIPLLKYVFVVCKANDNGQGSGTFALYSLLCRHAKVKTIQNQHRTDEELTTYS-RT 162

Query: 198 PNRRQKRASRIQSALENSHFAKVFLLFATMLGTAMVIGDGVLTPCISVLSAVGGITEATP 257
                  A++ +  LE     K  LL   ++GT MVIGDG+LTP ISVLSA GG+    P
Sbjct: 163 TFHEHSFAAKTKRWLEKRTSRKTALLILVLVGTCMVIGDGILTPAISVLSAAGGLRVNLP 222

Query: 258 AMTEGRIVWASVVILVLLFMAQRLGTDKVGYTFAPIIFIWFAFIAAIGVYNFFKYDPTVI 317
            ++ G +V+ +VVILV LF  Q  GTD+VG+ FAPI+F+WF  IA+IG+YN +K+D +V+
Sbjct: 223 HISNGVVVFVAVVILVSLFSVQHYGTDRVGWLFAPIVFLWFLSIASIGMYNIWKHDTSVL 282

Query: 318 KALNPAYIIYYFQRNKKDAWISLGGVVLCITGTEALFADVGHFSVRSIQLSMSLVTYPSL 377
           KA +P YI  YF+R  +D W SLGG++L ITG EALFAD+ HF V ++Q++ +++ +P L
Sbjct: 283 KAFSPVYIYRYFKRGGRDRWTSLGGIMLSITGIEALFADLSHFPVSAVQIAFTVIVFPCL 342

Query: 378 ICAYAGQSSFLRKHSDLVSHTFYKSTPGSLYWPMFVVAVLASIIASQAMISGTFSIIQQS 437
           + AY+GQ++++R++ D V+  FY+S PGS+YWPMF++A  A+I+ASQA IS TFS+++Q+
Sbjct: 343 LLAYSGQAAYIRRYPDHVADAFYRSIPGSVYWPMFIIATAAAIVASQATISATFSLVKQA 402

Query: 438 LSLGCFPRVRVIHTSAKHEGQVYVPEINYLLMLACVCVTLVFKNTAKIGNAYGIAVVFVM 497
           L+ GCFPRV+V+HTS K  GQ+YVP+IN++LM+ C+ VT  FKN ++IGNAYG AVV VM
Sbjct: 403 LAHGCFPRVKVVHTSRKFLGQIYVPDINWILMILCIAVTAGFKNQSQIGNAYGTAVVIVM 462

Query: 498 TLTSSFLVLIMVMIWKTHILLVITYVVIIGSVELVYLSSVLYKFDQGGYLPLAFAAVMMA 557
            +T+  + LIM+++W+ H +LV+ + V+   VE  Y S++L+K DQGG++PL  AA  + 
Sbjct: 463 LVTTLLMTLIMILVWRCHWVLVLIFTVLSLVVECTYFSAMLFKIDQGGWVPLVIAAAFLL 522

Query: 558 IMYVWNSVFRKKYFYELNHKISPEKFNEMVSTTSFRRLPGMAFFYTELVQGIPPIIKHYV 617
           IM+VW+    K+Y +E++ ++S      +  +    R+PG+   YTEL  G+P I  H++
Sbjct: 523 IMWVWHYGTLKRYEFEMHCRVSMAWILGLGPSLGLVRVPGVGLVYTELASGVPHIFSHFI 582

Query: 618 EHVPALQSVLVFVTIKSLPVSKVPADERFLFRRVEAKEIDVFRCVVRYGYTEVRAEHELF 677
            ++PA+ SV+VFV +K+LPV  VP +ERFL +R+  K   +FRCV RYGY ++  + + F
Sbjct: 583 TNLPAIHSVVVFVCVKNLPVYTVPEEERFLVKRIGPKNFHMFRCVARYGYRDLHKKDDDF 642

Query: 678 EKILLE------RLEKF------ESDRRALCNSDGRVEE------DEEDVNGF------- 737
           EK L E      RLE        +SD  ++C S  ++++      + E++  F       
Sbjct: 643 EKRLFESLFLYVRLESMMEGGCSDSDDYSICGSQQQLKDTLGNGNENENLATFDTFDSIE 702

Query: 738 ------------------GGVAE------AWKQGVVHFIGETEVVAKPGAGFGDRIMINY 776
                              GV E          GVVH +G T V A+  A F  +I I+Y
Sbjct: 703 SITPVKRVSNTVTASSQMSGVDELEFINGCRDAGVVHIMGNTVVRARREARFYKKIAIDY 762