Cp4.1LG03g04790 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g04790
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionHydroxymethylbilane synthase
LocationCp4.1LG03: 5288781 .. 5292330 (-)
RNA-Seq ExpressionCp4.1LG03g04790
SyntenyCp4.1LG03g04790
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAAGTGAAATGGATGAGAATCCTATCTGCCAACGCGAATGCAGAGATTTGGCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTTCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCAAATGGTTGCTTTATCTTCCCCTTCTGTAAGCCACTCTCCCATGGCTCGCCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCATTAATGCCCCCAAGAAACTTCATGGGCTTGGCCTCATAAGAGCCTCTGTTGCTGTGGAGCAGCAGGCAGGGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGGTATAACTTCTTTGTTTCGTGGGTCTTTTTTCGATTTTCTTTTGGTTTATGTTTTAGGATGTGTTCAGTTTCTAATCATGAGTTTGTGGCTGATTTGAGGGATAATGTAGGGCATTCAGTTGTTGTTTAAGGGTTTGGATCACTTCAATTTGTGCCTTTTAACTGTGATTAAGTTCTGATAAAGTGTTTATGCTGCTCATGCTATCAATCATGGCAGTGATAGTGTTTTACCTTACAATCTAAATGTTTGGATCCAATCTGGTGTTGTAGAGTAGAATCAGTTCTTCGTTTGAAGAGTAAAACTAATGTGAGATCCTACGTCGGTTGTGGAGGAGAATGGAACATTCTTTATAAGGGTGTGGAAACCCCTCCCTGGCATACGCATTTTAAAATCTTGAGGGGAAGCCCAGAAGGGGAAGTTCAAAGAGGACAATATATGTTAGTGGTGGGATTAGGTCGTTATAAATGGTATTGTCACTAGACATTGGGCGATGTGCCAGTGGAAGGATGAGCCCCGAAGGAGGGTGGACACGAGGCAGTGTGCCAACAAGAACGTTGGGCTACGAAGAGGTGTGGATTGGGGTCCCACGTTGATTTGAGAAGGGAACAAGTGCGAGAGAGGACGTTGGACCCTAAAGGGGGTGGATTGGGAGATCCCACATCGGTTGAGGAGAACAAAACATTCTCCATAAGAGTGTGGAAACTTCTCCGTAGCAAACGCGTTTTAAAACCTTGAGGGGAAGCCCGGAAGGGAAGCCCAGAGAGGACAATATTTACTAGCGATGGGCTTAGGTCGTTACAAATGGTATCAGAGCCAGACACCGGGCAATGTGCCAGTGGAAGGTTGAGCCCCGAAGGAGGGTGGACACGAGGCGGTGTGCTAGCAAGGTCATTGGGCTCAAAAGGGGTGTGGATTAGGGGTCTCACGTCGATTTGAGAAGAGAACAAGTGCCAGTGAGGACGCTGGGACCCAAAGGGGGTGGATTGTGAGATCCCACATCGGTTGGGGAGGGCGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTACCAAACGCGTTTTAAAAACCTTAAAGGGAAGCCTAGAAGGAAAAGCGTAAAGAGGACAAGGTTACTTCATTTTCTATATGCTTGTTTGATTGGGTTTACTTTATCTTCTTCTGTGTCAGGACAGCCCATTAGCACTAGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTAATCAAAACCACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAAAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGACGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTAAGTATGGTGTGTTATTTAATGGAAAAACATCCAAAAAGTTTATGTTCTACGTTTACATTGTTGTTGTGTATGTTGAATTCTGATTCTTGATGGAATGTTTATGTGTGAGATCCCACGTTGGTTGGAGAGGTGAACGAAGCATTTCTTATAAGGGTGTGGAAACCTCTTCCTAGTAGACTCGTTTTAAAATTTTGAGAGGAAGCCCTTGAAGGGAAAGCCCAAAGAGATCAGTATCTTCTAGCGATGGGCTTGGACTGTTACAAATGGTATTAGAATCAGACACTGGACGGTGTGCCAGCGAGTACACTGGGCCCCCAAGGGGGTGGATTGTAAGATCTCACATCGGTTAGAGAGAGGAACAAAGCATTCTTTATACGGGTGTGGAAACCTCTCACTAGCATACGCGTTTTAAAACCGTGAGATTGATAGCGATATGTAACGGGCCAAAACGGGCAATATCTACTAGCATGGTAGTGTTTAACGAGTCTTAAATCTTCAATCTTGTATGTTGGTTCTGATGCTCGCTCTCGTTTTTTCCATTGTTTGTTGGTGTCACTCGTTAGGTACTCGAGAATTTTCGGGGTAATGTCCAAACAAGGTTGAAAAAACTAAACGAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGTGCGTTTTCATTGGCTATACAAATCAATATTTGTCCATGAAAGTTCATTGCTTGAGAAACACAACTAAATGACATCTTATTTGAGCTGAACTTCGCCATTCCCGTTTGGTTTCTCATTTGCAGGCTGAGTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCGGGATATGCGTCGAGGGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCGGACGGAACCCGAGGTACGATCTCAAACGTTCATGTTCGAATGAAGTAACTTCTACATTGCTAGAACATTGAATATCTACATATTTTACAGTTCTTGAAACTTCTCGACGAGGTTCGTATGCCATCGACGATATGATAGCAATGGGGAAGGATGCTGGGAAGGAGCTACTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAGTTAGTTTAAAATTTAGGATAAAGATGAAGGCTTCATATCCAAATCTTCAAGTGAGTAGGAGTTATGTTCATCCTTTGTTTTGTTAATTGGTTGTGTAAATTAGAATAGAAATCATTTAGACTATCTTGGGTTCCTCAAATAAGTCTATGCAAACTAGCCATTTCTAATCCTCAAGCTCTATACAAACATGTGTTACTCTTTAATGGTCGTATTTGATCCGTTGATTGGATGATCGAACGCTCCGACCCGAACCGAAAGCTACCGAGCCTCTTCATGTTCACTTCTTCATTTACAAATGATACGTTTGGAATATAAACTCTCTTGTACCAGCTC

mRNA sequence

TGGAAGTGAAATGGATGAGAATCCTATCTGCCAACGCGAATGCAGAGATTTGGCCTGTTGGGTCATCAAAAACAATCCCTTCTAAGCTAACCCATCTCCTTTTTTCTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTCAAATGGTTGCTTTATCTTCCCCTTCTGTAAGCCACTCTCCCATGGCTCGCCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCATTAATGCCCCCAAGAAACTTCATGGGCTTGGCCTCATAAGAGCCTCTGTTGCTGTGGAGCAGCAGGCAGGGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGGATGTGTTCAGTTTCTAATCATGAGTTTGTGGCTGATTTGAGGGATAATGACAGCCCATTAGCACTAGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTAATCAAAACCACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAAAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGACGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTACTCGAGAATTTTCGGGGTAATGTCCAAACAAGGTTGAAAAAACTAAACGAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGCTGAGTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCGGGATATGCGTCGAGGGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCGGACGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTTCGTATGCCATCGACGATATGATAGCAATGGGGAAGGATGCTGGGAAGGAGCTACTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAGTTAGTTTAAAATTTAGGATAAAGATGAAGGCTTCATATCCAAATCTTCAAGTGAGTAGGAGTTATGTTCATCCTTTGTTTTGTTAATTGGTTGTGTAAATTAGAATAGAAATCATTTAGACTATCTTGGGTTCCTCAAATAAGTCTATGCAAACTAGCCATTTCTAATCCTCAAGCTCTATACAAACATGTGTTACTCTTTAATGGTCGTATTTGATCCGTTGATTGGATGATCGAACGCTCCGACCCGAACCGAAAGCTACCGAGCCTCTTCATGTTCACTTCTTCATTTACAAATGATACGTTTGGAATATAAACTCTCTTGTACCAGCTC

Coding sequence (CDS)

ATGGTTGCTTTATCTTCCCCTTCTGTAAGCCACTCTCCCATGGCTCGCCCTTGTAATCTTGCCTCCCTTTCTCTTCCTGGGTTCTCCTCACTTTCCCTTAAACCTCCGGCTTTCATTAATGCCCCCAAGAAACTTCATGGGCTTGGCCTCATAAGAGCCTCTGTTGCTGTGGAGCAGCAGGCAGGGAAGACCAAGGTTGCTCTCCTCAGAATTGGCACCAGAGGAAGGATGTGTTCAGTTTCTAATCATGAGTTTGTGGCTGATTTGAGGGATAATGACAGCCCATTAGCACTAGCCCAGGCTCATGAGACAAGAGACAAGCTCATGGCTTCCCATCCTGAGCTAGCTGAAGATGGTGCCATTCAGATTGTTGTAATCAAAACCACTGGTGACAAAATACTCTCTCAGCCACTTGCAGACATTGGTGGGAAAGGCTTATTTACAAAAGAGATTGATGATGCACTTATCAATGGTGACATAGACATTGCTGTTCACTCAATGAAAGATGTCCCAACTTACTTACCTGAAAAAACCATCCTCCCGTGTAACCTTCCGAGGGAGGACGTCCGGGACGCATTCATTTCTCTACGTGCAGGTTCACTTGCCGAGCTTCCAGCTGGAAGCGTCGTCGGTACAGCTTCACTGAGAAGAAAGTCTCAGCTACTGAATCGATATCCGTCCCTCGAAGTACTCGAGAATTTTCGGGGTAATGTCCAAACAAGGTTGAAAAAACTAAACGAAGGAGTAGTCCAAGCAACACTATTAGCTCTAGCTGGACTTCGACGTTTAAATATGACAGAAAACGTTACTTCGATCCTTTCAATCGATGAAATGCTTCCAGCTGTTGCTCAGGGAGCTATCGGTATCGCCTGTCGAAGCGACGATGACAAAATGGCTGAGTACTTAGCCTCATTAAACCATGAGGAAACAAGACTGGCTGTTGTTTGTGAGAGAGCTTTCCTCGAGACTCTTGATGGATCATGCCGAACCCCGATTGCGGGATATGCGTCGAGGGATGAAGATGGCAATTGTATATTCAAAGGGTTGGTAGCTTCCCCGGACGGAACCCGAGTTCTTGAAACTTCTCGACGAGGTTCGTATGCCATCGACGATATGATAGCAATGGGGAAGGATGCTGGGAAGGAGCTACTTTCTCGAGCAGGTCCGGGTTTTTTTGATAGCTAG

Protein sequence

MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQAGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Homology
BLAST of Cp4.1LG03g04790 vs. ExPASy Swiss-Prot
Match: Q43082 (Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.6e-155
Identity = 291/373 (78.02%), Postives = 320/373 (85.79%), Query Frame = 0

Query: 24  SLPGFSSLSLKPPAFINAPKKLHGLG--LIRASVAVEQQAGKTKVALLRIGTRGRMCSVS 83
           S P   SLSL   +F  +  K        IRAS+AVEQQ  + K AL+RIGTRG      
Sbjct: 15  SAPSNPSLSLFTSSFRFSSFKTSPFSKCRIRASLAVEQQTQQNKTALIRIGTRG------ 74

Query: 84  NHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQPLADI 143
                       SPLALAQAHETRDKLMASH ELAE+GAIQIV+IKTTGDKILSQPLADI
Sbjct: 75  ------------SPLALAQAHETRDKLMASHTELAEEGAIQIVIIKTTGDKILSQPLADI 134

Query: 144 GGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISLRAGSL 203
           GGKGLFTKEID+ALINGDIDIAVHSMKDVPTYLPE+TILPCNLPREDVRDAFISL A SL
Sbjct: 135 GGKGLFTKEIDEALINGDIDIAVHSMKDVPTYLPEETILPCNLPREDVRDAFISLSAASL 194

Query: 204 AELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLALAGLR 263
           A+LPAGSV+GTASLRRKSQ+L+RYPSL V +NFRGNVQTRL+KL+EGVV+ATLLALAGL+
Sbjct: 195 ADLPAGSVIGTASLRRKSQILHRYPSLTVQDNFRGNVQTRLRKLSEGVVKATLLALAGLK 254

Query: 264 RLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVCERAFL 323
           RLNMTENVTS LSID+MLPAVAQGAIGIACRS+DDKMAEYLASLNHEETRLA+ CERAFL
Sbjct: 255 RLNMTENVTSTLSIDDMLPAVAQGAIGIACRSNDDKMAEYLASLNHEETRLAISCERAFL 314

Query: 324 ETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMGKDAGK 383
            TLDGSCRTPIAGYASRD+DGNC+F+GLVASPDGTRVLETSR GSY  +DM+ +GKDAG+
Sbjct: 315 TTLDGSCRTPIAGYASRDKDGNCLFRGLVASPDGTRVLETSRIGSYTYEDMMKIGKDAGE 369

Query: 384 ELLSRAGPGFFDS 395
           ELLSRAGPGFF+S
Sbjct: 375 ELLSRAGPGFFNS 369

BLAST of Cp4.1LG03g04790 vs. ExPASy Swiss-Prot
Match: Q43316 (Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC PE=1 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 1.4e-151
Identity = 286/399 (71.68%), Postives = 319/399 (79.95%), Query Frame = 0

Query: 2   VALSSPSVSH--------SPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRA 61
           +A SS S +H        S     C+L S+S  GFS   +  PA     +K    G ++A
Sbjct: 3   IASSSLSQAHKVVLTRQPSSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKA 62

Query: 62  SVAVEQQAGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHP 121
            VAVEQ   KT+ A++RIGTRG                  SPLALAQA+ETR+KL   HP
Sbjct: 63  CVAVEQ---KTRTAIIRIGTRG------------------SPLALAQAYETREKLKKKHP 122

Query: 122 ELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTY 181
           EL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTY
Sbjct: 123 ELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTY 182

Query: 182 LPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLEN 241
           LPEKTILPCNLPREDVRDAFI L A +LAELPAGSVVGTASLRRKSQ+L++YP+L V EN
Sbjct: 183 LPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEEN 242

Query: 242 FRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRS 301
           FRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+
Sbjct: 243 FRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRT 302

Query: 302 DDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASP 361
           DDDKMA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASP
Sbjct: 303 DDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASP 362

Query: 362 DGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFF 393
           DGT+VLETSR+G Y  +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 363 DGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

BLAST of Cp4.1LG03g04790 vs. ExPASy Swiss-Prot
Match: Q6H6D2 (Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HEMC PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-135
Identity = 251/344 (72.97%), Postives = 285/344 (82.85%), Query Frame = 0

Query: 51  IRASVAVEQQAGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMA 110
           +RA+VAV+ +A + KV+L+RIGTRG                  SPLALAQAHETRDKL A
Sbjct: 33  VRAAVAVQAEA-QAKVSLIRIGTRG------------------SPLALAQAHETRDKLKA 92

Query: 111 SHPELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDV 170
           +H ELAE+GA++IV+IKTTGD IL +PLADIGGKGLFTKEIDDAL+ G IDIAVHSMKDV
Sbjct: 93  AHSELAEEGAVEIVIIKTTGDMILDKPLADIGGKGLFTKEIDDALLQGRIDIAVHSMKDV 152

Query: 171 PTYLPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEV 230
           PTYLPE TILPCNLPREDVRDAFI L A SLAELPAGSVVG+ASLRR+SQ+L +YPSL+V
Sbjct: 153 PTYLPEGTILPCNLPREDVRDAFICLTASSLAELPAGSVVGSASLRRQSQILYKYPSLKV 212

Query: 231 LENFRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIA 290
           + NFRGNVQTRL+KL EG V ATLLALAGL+RLNM E  TS+LS+DEMLPAVAQGAIGIA
Sbjct: 213 V-NFRGNVQTRLRKLKEGDVHATLLALAGLKRLNMAETATSVLSVDEMLPAVAQGAIGIA 272

Query: 291 CRSDDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLV 350
           CRS DD M  YL+SLNHE+TRLAV CER FL  LDG+CRTPIA YASRD+DGNC F+GL+
Sbjct: 273 CRSSDDTMMNYLSSLNHEDTRLAVACEREFLSVLDGNCRTPIAAYASRDKDGNCSFRGLL 332

Query: 351 ASPDGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 395
           ASPDG+ V ETSR G Y  D M+ MGKDAG EL ++AGPGFFDS
Sbjct: 333 ASPDGSTVYETSRTGPYDFDIMVEMGKDAGHELKAKAGPGFFDS 356

BLAST of Cp4.1LG03g04790 vs. ExPASy Swiss-Prot
Match: Q3SVF3 (Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=hemC PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.9e-82
Identity = 169/316 (53.48%), Postives = 212/316 (67.09%), Query Frame = 0

Query: 77  MCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKTTGDKILSQ 136
           M S    + +A +    SPLALAQAHE RD+L  +H    E   I I  I+T+GD I  +
Sbjct: 1   MQSSDETDILATIGTRGSPLALAQAHEVRDRLARAHQVAPE--RIAIKTIRTSGDAIQDR 60

Query: 137 PLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPREDVRDAFISL 196
           PL D+GGKGLFTKEI++AL+ G ID AVHS KDVPT+LP+ T LP  LPREDVRD FIS 
Sbjct: 61  PLFDVGGKGLFTKEIEEALLAGTIDFAVHSSKDVPTFLPDATWLPAFLPREDVRDVFISP 120

Query: 197 RAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEGVVQATLLA 256
            AGSL +LPAG+ VGTASLRR++ +L   P L+V  + RGNV+TRL+K++ G   ATLLA
Sbjct: 121 HAGSLNDLPAGATVGTASLRRQAMVLKLRPDLKV-NSLRGNVETRLRKISVGEADATLLA 180

Query: 257 LAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHEETRLAVVC 316
           LAGL RL + +  T IL  DE LPAV QGAI I  R DDD++  ++ ++   ET +A+  
Sbjct: 181 LAGLNRLGLQDKATRILETDEFLPAVGQGAIAIESRRDDDRINAFVKAIGDPETEVALSA 240

Query: 317 ERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYAIDDMIAMG 376
           ER+FL  LDGSCRTPI G+   + D    F+GL+ SPDGT   ET+R G+ A  D  A+G
Sbjct: 241 ERSFLALLDGSCRTPIGGHCRVNGD-RIDFRGLIISPDGTEFYETTREGARA--DAAALG 300

Query: 377 KDAGKELLSRAGPGFF 393
            DA  EL  RAG  FF
Sbjct: 301 ADAAHELRERAGEKFF 310

BLAST of Cp4.1LG03g04790 vs. ExPASy Swiss-Prot
Match: A1B853 (Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586 GN=hemC PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.9e-82
Identity = 175/325 (53.85%), Postives = 220/325 (67.69%), Query Frame = 0

Query: 69  LRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGAIQIVVIKT 128
           +RIGTRG                  S LALAQAHETRD+LMA+H  LA D A +IVVIKT
Sbjct: 11  IRIGTRG------------------SALALAQAHETRDRLMAAH-GLAAD-AFRIVVIKT 70

Query: 129 TGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTILPCNLPRED 188
           TGD++L +PL +IGGKGLFT+EI+DAL+  +IDIAVHSMKD+PT  PE  ++ C LPRED
Sbjct: 71  TGDRVLDRPLKEIGGKGLFTREIEDALLAHEIDIAVHSMKDMPTIQPEGLVIDCYLPRED 130

Query: 189 VRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQTRLKKLNEG 248
           VRDAF+S +  +++ELP G+VVG++SLRR++QL  R P L+++E FRGNVQTRLKKL +G
Sbjct: 131 VRDAFVSAQFAAISELPQGAVVGSSSLRRRAQLAARRPDLKLVE-FRGNVQTRLKKLEDG 190

Query: 249 VVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAEYLASLNHE 308
           V  AT LA+AGL RL M       +  DEMLPAVAQG IG+  R+DD + A  LA+++  
Sbjct: 191 VAVATFLAMAGLTRLGMLHVARGAVEPDEMLPAVAQGCIGVERRADDARTASLLAAISDR 250

Query: 309 ETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLETSRRGSYA 368
           ++ L V  ERAFL  LDGSC+TPIAG A    D     +G +  PDG+ V+   R G  A
Sbjct: 251 DSALRVTAERAFLARLDGSCQTPIAGLAELQGD-RLRLRGEILRPDGSEVIAAERVGPAA 310

Query: 369 IDDMIAMGKDAGKELLSRAGPGFFD 394
             D  AMG D  +EL  RA   FFD
Sbjct: 311 --DGAAMGTDLAEELRGRAPADFFD 311

BLAST of Cp4.1LG03g04790 vs. NCBI nr
Match: XP_023529085.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 716 bits (1847), Expect = 3.08e-259
Identity = 376/394 (95.43%), Postives = 376/394 (95.43%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
           AGKTKVALLRIGTRG                  SPLALAQAHETRDKLMASHPELAEDGA
Sbjct: 61  AGKTKVALLRIGTRG------------------SPLALAQAHETRDKLMASHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE
Sbjct: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. NCBI nr
Match: XP_022934624.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 709 bits (1829), Expect = 1.71e-256
Identity = 372/394 (94.42%), Postives = 372/394 (94.42%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           MVALSSPSVSHSPMA PCNLASLSLPGFSSLSLKPPAF N PKKLHG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
           AGKTKVALLRIGTRG                  SPLALAQAHETRDKLMASHPELAEDGA
Sbjct: 61  AGKTKVALLRIGTRG------------------SPLALAQAHETRDKLMASHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE
Sbjct: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. NCBI nr
Match: KAG6581556.1 (Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 707 bits (1825), Expect = 2.99e-255
Identity = 371/394 (94.16%), Postives = 372/394 (94.42%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           MVALSSPSVSHSPMA PCNLASLSLPGFSSLSLKPPAF N PKKLHG GLIRASVAVEQQ
Sbjct: 40  MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 99

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
           AGKTKVALLRIGTRG                  SPLALAQAHETRDKLMASHPELAEDGA
Sbjct: 100 AGKTKVALLRIGTRG------------------SPLALAQAHETRDKLMASHPELAEDGA 159

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 160 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 219

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT
Sbjct: 220 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 279

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RLKKLN+GVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE
Sbjct: 280 RLKKLNKGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 339

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 340 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 399

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 400 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 415

BLAST of Cp4.1LG03g04790 vs. NCBI nr
Match: XP_022982636.1 (porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 701 bits (1809), Expect = 1.90e-253
Identity = 369/394 (93.65%), Postives = 370/394 (93.91%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           MVALSSPSVS+SPMARPCNLASLSLPGFSSLSLKPPAF NAPKK HG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
             KTKVALLRIGTRG                  SPLALAQAHETRDKLMASHPELAEDGA
Sbjct: 61  PEKTKVALLRIGTRG------------------SPLALAQAHETRDKLMASHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA 
Sbjct: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAN 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. NCBI nr
Match: XP_038906286.1 (porphobilinogen deaminase, chloroplastic [Benincasa hispida])

HSP 1 Score: 672 bits (1733), Expect = 7.22e-242
Identity = 353/394 (89.59%), Postives = 360/394 (91.37%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           M  LSSPSVSHSPM RP N+ SLSL GFSSLSLKPPAF N  KK HG GLIRASVAVEQQ
Sbjct: 1   MGVLSSPSVSHSPMPRPRNVGSLSLLGFSSLSLKPPAFSNGAKKFHGFGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
             KTKVAL+RIGTRG                  SPLALAQAHETRDKLMASHPELAEDGA
Sbjct: 61  TEKTKVALVRIGTRG------------------SPLALAQAHETRDKLMASHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISL AGSLAELPAGS++GTASLRRKSQLLNRYPSL+VLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLSAGSLAELPAGSIIGTASLRRKSQLLNRYPSLKVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RL+KLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACR+DDDKMA 
Sbjct: 241 RLRKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRTDDDKMAN 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRG YAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGPYAIDDMIAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. ExPASy TrEMBL
Match: A0A6J1F354 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 SV=1)

HSP 1 Score: 709 bits (1829), Expect = 8.26e-257
Identity = 372/394 (94.42%), Postives = 372/394 (94.42%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           MVALSSPSVSHSPMA PCNLASLSLPGFSSLSLKPPAF N PKKLHG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSHSPMAHPCNLASLSLPGFSSLSLKPPAFTNGPKKLHGFGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
           AGKTKVALLRIGTRG                  SPLALAQAHETRDKLMASHPELAEDGA
Sbjct: 61  AGKTKVALLRIGTRG------------------SPLALAQAHETRDKLMASHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE
Sbjct: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. ExPASy TrEMBL
Match: A0A6J1IZW2 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV=1)

HSP 1 Score: 701 bits (1809), Expect = 9.22e-254
Identity = 369/394 (93.65%), Postives = 370/394 (93.91%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           MVALSSPSVS+SPMARPCNLASLSLPGFSSLSLKPPAF NAPKK HG GLIRASVAVEQQ
Sbjct: 1   MVALSSPSVSNSPMARPCNLASLSLPGFSSLSLKPPAFTNAPKKFHGFGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
             KTKVALLRIGTRG                  SPLALAQAHETRDKLMASHPELAEDGA
Sbjct: 61  PEKTKVALLRIGTRG------------------SPLALAQAHETRDKLMASHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMA 
Sbjct: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAN 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. ExPASy TrEMBL
Match: A0A6J1GCC7 (Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 SV=1)

HSP 1 Score: 665 bits (1716), Expect = 1.36e-239
Identity = 349/394 (88.58%), Postives = 355/394 (90.10%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           M   SSPS+SH PMARPCNL SLS  GFSSLSLKPPAF    KK HG GLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGGKKFHGFGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
           A KTKVAL+RIGTRG                  SPLALAQAHETR+KLMA HPELAEDGA
Sbjct: 61  AEKTKVALVRIGTRG------------------SPLALAQAHETREKLMAIHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIV+IKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVIIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISL  GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLSVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RL+KLNEGVVQATLLALAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMA 
Sbjct: 241 RLRKLNEGVVQATLLALAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMAN 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRG YAIDDMIAMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGPYAIDDMIAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. ExPASy TrEMBL
Match: A0A6J1KA97 (Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV=1)

HSP 1 Score: 664 bits (1714), Expect = 2.74e-239
Identity = 347/394 (88.07%), Postives = 356/394 (90.36%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           M   SSPS+SH PMARPCNL SLS  GFSSLSLKPPAF    K+ HG+GLIRASVAVEQQ
Sbjct: 1   MGVFSSPSISHYPMARPCNLGSLSRHGFSSLSLKPPAFSTGDKRFHGVGLIRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
           A KTKVAL+RIGTRG                  SPLALAQAHETR+KLMA HPELAEDGA
Sbjct: 61  AEKTKVALVRIGTRG------------------SPLALAQAHETREKLMAIHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQIV+IKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIVIIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISL  GSLAELPAGSV+GTASLRRKSQLLNRYPSL VLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLSVGSLAELPAGSVIGTASLRRKSQLLNRYPSLNVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RL+KLNEGVVQATLLALAGLRRLNMTE VTSILSIDEMLPAVAQGAIGIACRSDDDKMA 
Sbjct: 241 RLRKLNEGVVQATLLALAGLRRLNMTETVTSILSIDEMLPAVAQGAIGIACRSDDDKMAN 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRG YAIDDM+AMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGPYAIDDMVAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. ExPASy TrEMBL
Match: A0A6J1DII9 (Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3 SV=1)

HSP 1 Score: 656 bits (1693), Expect = 4.33e-236
Identity = 341/394 (86.55%), Postives = 357/394 (90.61%), Query Frame = 0

Query: 1   MVALSSPSVSHSPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRASVAVEQQ 60
           M   SSP ++HSPMARPCN+ SLS  GFSSLSLKPPAF N  KK  GLGL+RASVAVEQQ
Sbjct: 1   MGVFSSPPITHSPMARPCNVGSLSPLGFSSLSLKPPAFCNGGKKFGGLGLVRASVAVEQQ 60

Query: 61  AGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHPELAEDGA 120
             KTKVAL+RIGTRG                  SPLALAQA+ETRDKLMA HPELAEDGA
Sbjct: 61  TEKTKVALVRIGTRG------------------SPLALAQAYETRDKLMAIHPELAEDGA 120

Query: 121 IQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTYLPEKTIL 180
           IQI++IKTTGDKILSQPLADIGGKGLFTKEIDDALIN DIDIAVHSMKDVPTYLPEKTIL
Sbjct: 121 IQIIIIKTTGDKILSQPLADIGGKGLFTKEIDDALINSDIDIAVHSMKDVPTYLPEKTIL 180

Query: 181 PCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLENFRGNVQT 240
           PCNLPREDVRDAFISL AGSLAELPAGSV+GTASLRRKSQLLNRYPSL+VLENFRGNVQT
Sbjct: 181 PCNLPREDVRDAFISLSAGSLAELPAGSVIGTASLRRKSQLLNRYPSLKVLENFRGNVQT 240

Query: 241 RLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRSDDDKMAE 300
           RL+KLNEGVVQATLLALAGLRRLNMTENVTS+LSIDEMLPAVAQGAIGIACRS+DDKMA 
Sbjct: 241 RLRKLNEGVVQATLLALAGLRRLNMTENVTSVLSIDEMLPAVAQGAIGIACRSNDDKMAN 300

Query: 301 YLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASPDGTRVLE 360
           YLASLNHEETRLAVVCERAFLE LDGSCRTPIAGYAS+DEDGNCIFKGLVASPDGTRVLE
Sbjct: 301 YLASLNHEETRLAVVCERAFLEMLDGSCRTPIAGYASKDEDGNCIFKGLVASPDGTRVLE 360

Query: 361 TSRRGSYAIDDMIAMGKDAGKELLSRAGPGFFDS 394
           TSRRG+YAIDDM+AMGKDAGKELLSRAGPGFFDS
Sbjct: 361 TSRRGAYAIDDMVAMGKDAGKELLSRAGPGFFDS 376

BLAST of Cp4.1LG03g04790 vs. TAIR 10
Match: AT5G08280.1 (hydroxymethylbilane synthase )

HSP 1 Score: 537.3 bits (1383), Expect = 1.0e-152
Identity = 286/399 (71.68%), Postives = 319/399 (79.95%), Query Frame = 0

Query: 2   VALSSPSVSH--------SPMARPCNLASLSLPGFSSLSLKPPAFINAPKKLHGLGLIRA 61
           +A SS S +H        S     C+L S+S  GFS   +  PA     +K    G ++A
Sbjct: 3   IASSSLSQAHKVVLTRQPSSRVNTCSLGSVSAIGFSLPQISSPALGKCRRKQSSSGFVKA 62

Query: 62  SVAVEQQAGKTKVALLRIGTRGRMCSVSNHEFVADLRDNDSPLALAQAHETRDKLMASHP 121
            VAVEQ   KT+ A++RIGTRG                  SPLALAQA+ETR+KL   HP
Sbjct: 63  CVAVEQ---KTRTAIIRIGTRG------------------SPLALAQAYETREKLKKKHP 122

Query: 122 ELAEDGAIQIVVIKTTGDKILSQPLADIGGKGLFTKEIDDALINGDIDIAVHSMKDVPTY 181
           EL EDGAI I +IKTTGDKILSQPLADIGGKGLFTKEID+ALING IDIAVHSMKDVPTY
Sbjct: 123 ELVEDGAIHIEIIKTTGDKILSQPLADIGGKGLFTKEIDEALINGHIDIAVHSMKDVPTY 182

Query: 182 LPEKTILPCNLPREDVRDAFISLRAGSLAELPAGSVVGTASLRRKSQLLNRYPSLEVLEN 241
           LPEKTILPCNLPREDVRDAFI L A +LAELPAGSVVGTASLRRKSQ+L++YP+L V EN
Sbjct: 183 LPEKTILPCNLPREDVRDAFICLTAATLAELPAGSVVGTASLRRKSQILHKYPALHVEEN 242

Query: 242 FRGNVQTRLKKLNEGVVQATLLALAGLRRLNMTENVTSILSIDEMLPAVAQGAIGIACRS 301
           FRGNVQTRL KL  G VQATLLALAGL+RL+MTENV SILS+DEMLPAVAQGAIGIACR+
Sbjct: 243 FRGNVQTRLSKLQGGKVQATLLALAGLKRLSMTENVASILSLDEMLPAVAQGAIGIACRT 302

Query: 302 DDDKMAEYLASLNHEETRLAVVCERAFLETLDGSCRTPIAGYASRDEDGNCIFKGLVASP 361
           DDDKMA YLASLNHEETRLA+ CERAFLETLDGSCRTPIAGYAS+DE+GNCIF+GLVASP
Sbjct: 303 DDDKMATYLASLNHEETRLAISCERAFLETLDGSCRTPIAGYASKDEEGNCIFRGLVASP 362

Query: 362 DGTRVLETSRRGSYAIDDMIAMGKDAGKELLSRAGPGFF 393
           DGT+VLETSR+G Y  +DM+ MGKDAG+ELLSRAGPGFF
Sbjct: 363 DGTKVLETSRKGPYVYEDMVKMGKDAGQELLSRAGPGFF 380

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q430821.6e-15578.02Porphobilinogen deaminase, chloroplastic OS=Pisum sativum OX=3888 GN=HEMC PE=1 S... [more]
Q433161.4e-15171.68Porphobilinogen deaminase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HEMC... [more]
Q6H6D21.4e-13572.97Porphobilinogen deaminase, chloroplastic OS=Oryza sativa subsp. japonica OX=3994... [more]
Q3SVF36.9e-8253.48Porphobilinogen deaminase OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 1... [more]
A1B8536.9e-8253.85Porphobilinogen deaminase OS=Paracoccus denitrificans (strain Pd 1222) OX=318586... [more]
Match NameE-valueIdentityDescription
XP_023529085.13.08e-25995.43porphobilinogen deaminase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022934624.11.71e-25694.42porphobilinogen deaminase, chloroplastic-like [Cucurbita moschata][more]
KAG6581556.12.99e-25594.16Porphobilinogen deaminase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_022982636.11.90e-25393.65porphobilinogen deaminase, chloroplastic-like [Cucurbita maxima][more]
XP_038906286.17.22e-24289.59porphobilinogen deaminase, chloroplastic [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1F3548.26e-25794.42Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111441758 PE=3 ... [more]
A0A6J1IZW29.22e-25493.65Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111481450 PE=3 SV... [more]
A0A6J1GCC71.36e-23988.58Hydroxymethylbilane synthase OS=Cucurbita moschata OX=3662 GN=LOC111452689 PE=3 ... [more]
A0A6J1KA972.74e-23988.07Hydroxymethylbilane synthase OS=Cucurbita maxima OX=3661 GN=LOC111493083 PE=3 SV... [more]
A0A6J1DII94.33e-23686.55Hydroxymethylbilane synthase OS=Momordica charantia OX=3673 GN=LOC111020357 PE=3... [more]
Match NameE-valueIdentityDescription
AT5G08280.11.0e-15271.68hydroxymethylbilane synthase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000860Porphobilinogen deaminasePRINTSPR00151PORPHBDMNASEcoord: 130..150
score: 66.21
coord: 161..180
score: 61.19
coord: 209..226
score: 57.41
coord: 229..246
score: 52.38
coord: 316..333
score: 53.17
IPR000860Porphobilinogen deaminaseTIGRFAMTIGR00212TIGR00212coord: 93..385
e-value: 1.3E-96
score: 321.1
IPR000860Porphobilinogen deaminasePANTHERPTHR11557PORPHOBILINOGEN DEAMINASEcoord: 15..393
IPR000860Porphobilinogen deaminaseHAMAPMF_00260Porphobil_deamcoord: 86..386
score: 32.881744
IPR036803Porphobilinogen deaminase, C-terminal domain superfamilyGENE3D3.30.160.40coord: 307..393
e-value: 4.5E-24
score: 86.7
IPR036803Porphobilinogen deaminase, C-terminal domain superfamilySUPERFAMILY54782Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domaincoord: 306..393
NoneNo IPR availableGENE3D3.40.190.10coord: 93..296
e-value: 1.7E-77
score: 261.4
NoneNo IPR availableGENE3D3.40.190.10coord: 188..288
e-value: 1.7E-77
score: 261.4
NoneNo IPR availablePANTHERPTHR11557:SF8BNAC02G01240D PROTEINcoord: 15..393
NoneNo IPR availableCDDcd13648PBP2_PBGD_1coord: 68..365
e-value: 0.0
score: 501.942
NoneNo IPR availableSUPERFAMILY53850Periplasmic binding protein-like IIcoord: 93..304
IPR022418Porphobilinogen deaminase, C-terminalPFAMPF03900Porphobil_deamCcoord: 312..384
e-value: 2.4E-15
score: 56.6
IPR022417Porphobilinogen deaminase, N-terminalPFAMPF01379Porphobil_deamcoord: 93..299
e-value: 3.7E-72
score: 242.2
IPR022419Porphobilinogen deaminase, dipyrromethane cofactor binding sitePROSITEPS00533PORPHOBILINOGEN_DEAMcoord: 317..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g04790.1Cp4.1LG03g04790.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018160 peptidyl-pyrromethane cofactor linkage
biological_process GO:0006782 protoporphyrinogen IX biosynthetic process
biological_process GO:0050790 regulation of catalytic activity
biological_process GO:0017157 regulation of exocytosis
biological_process GO:0033014 tetrapyrrole biosynthetic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005096 GTPase activator activity
molecular_function GO:0004418 hydroxymethylbilane synthase activity
molecular_function GO:0045159 myosin II binding
molecular_function GO:0019905 syntaxin binding