Cp4.1LG03g04710 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g04710
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	structure-specific endonuclease subunit slx1
Location	Cp4.1LG03: 5292865 .. 5295774 (+)
RNA-Seq Expression	Cp4.1LG03g04710
Synteny	Cp4.1LG03g04710

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCTATGTCCTAAAACTAAATCAAATTAAATCCGGGGGAAAAACGGTCAACCAAACGGGAAGGTTCGAACCCGCGCCGATGAACGAAGACTATGATGAGGCTGCTCTCTCAGACATTTCGTAGCCAAAAACTCCCAATTTCAAGCTCAGAAGTTTCGAAATCTTCTTCTTCTGCATCAACTAAGGTTCCAACATTGATCGTCAAATCAGAGCCGGAGCCGAAGAAATGGTGCGTTTATCTCATCATCTCCTCCAATCCCCCCATCAAAACCTATGTAGGAGTCACCCTCGACTTTGATCGACGGTATGTCGAATTTCTTCTCTTGCGTTTCTTGTTTGAATCCCAGATTCCATGATTTGGTGTTGAAATTTTTTCTAGGTCGTGTTCGATTTAGCCTTGAAAAGTGATATTTCGAGCTTTTAGGTTAAACATTTGGCTTCATATTGTTGAATAATCTGACTCGATCAAACTCACACACCCATGAACAGAAATTAAACAAAGAGATACAAAGATTTACGTGATTCATCCACGGACTGCAACCAATCACAATAATCAAAGTGTATATACAAAGTCTCACTTCCTCTCAAATGCCACTAGGTAAATACAAAATGACACTCAATCTCAATAACCATCCTAGAAGATCATTTATATAAGCATACAAGATGATTCATGTATCAATATGAGAGATGAACCATATGAATTTCAGTAGGTGTATGCATAGGAGAAGGGAATAGAAGAAGGGAATGTACCAAAAATGTTCATGAATCTGATTCTTTATGTCAATTTACAACCGTTACTAGGTGAATAAGTGCAATTTCTTTGAAAAATATGCTCAAATTGTGTCACTTGTTATGGATATAACTTTGTATTGAGTCAATTTGGTGGATAAAGACGAGGTTGGTCTTTGAAAAAGAACTTGGGTTTAGATGCTGAACTGCTTGATTAATCAGTTGAGGTATTGGTTTTCTTTCGAGCTTGTAAGATAGGGAGTGTTTTGGGATCATGCCCACACTGTTGTGAGATATGCTTCAAATTTGCACTTTGCAGCTTTTACATGTCCTTTCTGCATAAGCGGTATTCAAATATTTCTTAATGTCACGAAGTTGTATTCTTGTACAGTTTAAAACAACATAATGGTGAAATAAAAGGTGGTGCAAAGGCAACTCGAGCGGGGCGACCCTGGATTTGTGCGTGCAAAATTCATGGTTTTAAAGACCAAAGTCAAGGTATCCATTTAAAATAGAATTTCAAGGGCATTGCCTGCCTATTTGTGATTGTAGTCATTCTAGACATTATGTTGCTTAGGGCTCTTGAACAGGTGAGATATTTCCTAGTTTCTTTGTTTTGGTAGTGATCTTCCTAAGTTAGATGTAACAGTCCAAGCCACCGCTAGCACTGCTAGCAGATATTGTTCTCTTTGAACTTTCCTTTTCGGACCAGAACCTTAACCCGACACCTTTGACCAACTCGGCCATCTAATGGTATCAGAGCCAGGCATCGAGCGGTGTGCCAGCGAGGACGCTGGGCCCCCAAGGGGGGTGGATTGTGATATCCCACATCAGTTGGAGAGGGGAACGAAACATTCTCTATAAGGGTGTGAAAAATTCTCCCTAGCAAACGCGTTTTAAAACCATGAGGCTAATGGCGATATGTAACGGGCCAAAGCGGACAATATCTTCTAGTGGTGAACTTGGGCTGCTACATTAGACATCAATGGTCAAATATATTTCAGATTTTCTCGTATTGTGAGTACTATTTTCCTGAGAAACAATCTTTATTCATTGCTCCACGCTGATATTTATTATATGATGCATTTTAATCATCATGGATTGACATAGTTGTCAACAAAGACCATGTAAATAATGAAGAACTTGGAGAGAATGAGTTCAAATTATGGTGCCGATCTATGTAGAATATTGTACATAGGGTGAGATTGTTGTCCGGTGAGAATAGCTGAGGTGTGCATAAGTTGTGCTAGTCTGGTAATCTCCCGCTCCCAAATTATATGCATGTTAGGACTCATACCTTCCAAATGGGGCCTTCCATCTTTAGTGTATTTTGATTTGATTTTTCCCCCTGGAAATGAAACAAGCTGCAACTTATAGCATTTCTGATTTAAAAAGCAGCTTGAAAGCCCCCTTGTTTTTCAGTTTTTTTTATTTACTAAACTGATAAAGGAAAGAAGGCGGGTCGTTATGGCCAATTAGGCAAACCATGCCAATGCACTAGTCAGCCGGTGTGGTGGCGAACACATTGTGCAGGTGGCCTTTCTCCTAGACTGTTCAGGAACATAGATCCGCAGTAGCTCACTTGTTGAAATAGTCACAGTGACAATTGGAGGTTGCTGGGTGAGACCAAGAGAAAGAAAGGACGGGAGGCAAGGGGCGTCAGTAATGGCCCCATGCGTAGGCGTCTGGACTTGTTGCTATGAACATTCTTCACGTTCATATCTCAAGAGCCTGTTTTGACCGACTTTCCAAGAGCTTAAAAATAAGATTGGTTTCACTCAAAACACATTTTAAACACTTAGAAATCATGTTTTGAAGCTCTATTTGGACCACCAACCTGATAATCAAATTTCTCTAGAGCTTTGGAACCGAGAAATGTTGGATAAAATCATTCCCACACCATTACTGATGCTGTTTTTGTATACTTTGGTATGCAGCTTGTGAGTTTGAATCAAAATGGAAAGAAATTTGCAGGAAAATGTGCCATATGAGAAAGGAAGAAGTAGATGATCAGACATTAAGATTGCTGAAGCAAAGACAGAGAGCTCTGGGAAAAGTGAAAGGTCTGTTTGATTGCACTCAATTTGAGTTTGATTGGGAACTCGATCCTTTCTGATTCTTTTACAATATTTATCAAAGTTGTTTATTAGATCAAATCTTCTTAAACTTTACCTTTCATAAA

mRNA sequence

TGCTATGTCCTAAAACTAAATCAAATTAAATCCGGGGGAAAAACGGTCAACCAAACGGGAAGGTTCGAACCCGCGCCGATGAACGAAGACTATGATGAGGCTGCTCTCTCAGACATTTCGTAGCCAAAAACTCCCAATTTCAAGCTCAGAAGTTTCGAAATCTTCTTCTTCTGCATCAACTAAGGTTCCAACATTGATCGTCAAATCAGAGCCGGAGCCGAAGAAATGGTGCGTTTATCTCATCATCTCCTCCAATCCCCCCATCAAAACCTATGTAGGAGTCACCCTCGACTTTGATCGACGTTTAAAACAACATAATGGTGAAATAAAAGGTGGTGCAAAGGCAACTCGAGCGGGGCGACCCTGGATTTGTGCGTGCAAAATTCATGGTTTTAAAGACCAAAGTCAAGCTTGTGAGTTTGAATCAAAATGGAAAGAAATTTGCAGGAAAATGTGCCATATGAGAAAGGAAGAAGTAGATGATCAGACATTAAGATTGCTGAAGCAAAGACAGAGAGCTCTGGGAAAAGTGAAAGGTCTGTTTGATTGCACTCAATTTGAGTTTGATTGGGAACTCGATCCTTTCTGATTCTTTTACAATATTTATCAAAGTTGTTTATTAGATCAAATCTTCTTAAACTTTACCTTTCATAAA

Coding sequence (CDS)

ATGATGAGGCTGCTCTCTCAGACATTTCGTAGCCAAAAACTCCCAATTTCAAGCTCAGAAGTTTCGAAATCTTCTTCTTCTGCATCAACTAAGGTTCCAACATTGATCGTCAAATCAGAGCCGGAGCCGAAGAAATGGTGCGTTTATCTCATCATCTCCTCCAATCCCCCCATCAAAACCTATGTAGGAGTCACCCTCGACTTTGATCGACGTTTAAAACAACATAATGGTGAAATAAAAGGTGGTGCAAAGGCAACTCGAGCGGGGCGACCCTGGATTTGTGCGTGCAAAATTCATGGTTTTAAAGACCAAAGTCAAGCTTGTGAGTTTGAATCAAAATGGAAAGAAATTTGCAGGAAAATGTGCCATATGAGAAAGGAAGAAGTAGATGATCAGACATTAAGATTGCTGAAGCAAAGACAGAGAGCTCTGGGAAAAGTGAAAGGTCTGTTTGATTGCACTCAATTTGAGTTTGATTGGGAACTCGATCCTTTCTGA

Protein sequence

MMRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKMCHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF

Homology

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy Swiss-Prot
Match: B6JY16 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces japonicus (strain yFS275 / FY16936) OX=402676 GN=slx1 PE=3 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.4e-13
Identity = 43/112 (38.39%), Postives = 60/112 (53.57%), Query Frame = 0


Query: 46  WCVYLIISSNPPIKT-YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQ 105
           +C YL++S     ++ Y+G T D  RRL+QHNGEIKGGA  T+  RPW  AC +HGF  +
Sbjct: 3   YCCYLLVSEKAASRSVYIGSTPDPARRLRQHNGEIKGGAYKTKRSRPWKVACFVHGFPTK 62

Query: 106 SQACEFESKWKEICRKMCHMRKEEVDDQTLRLLKQRQRALG-KVKGLFDCTQ 156
             A +FE  W+       H +    DD     L+Q  R +  K + L  C +
Sbjct: 63  IAALQFEWVWQ-------HPQSTRHDD-----LRQHSRVVSVKRQTLLSCVR 102

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy Swiss-Prot
Match: Q9P7M3 (Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=slx1 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.2e-12
Identity = 32/74 (43.24%), Postives = 46/74 (62.16%), Query Frame = 0


Query: 46  WCVYLIISSNPPIK--TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKD 105
           +C YL+ S+        Y+G T D  RRL+QHNGEI GGA  T+ GRPW  +C ++GF +
Sbjct: 7   YCCYLLKSNRTQSSGAVYIGSTPDPPRRLRQHNGEIVGGASKTKHGRPWSISCLVYGFPN 66

Query: 106 QSQACEFESKWKEI 118
           +  A +FE  W+ +
Sbjct: 67  KVSALKFEWNWQNL 80

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy Swiss-Prot
Match: B8MDD1 (Structure-specific endonuclease subunit slx1 OS=Talaromyces stipitatus (strain ATCC 10500 / CBS 375.48 / QM 6759 / NRRL 1006) OX=441959 GN=slx1 PE=3 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 6.0e-11
Identity = 36/91 (39.56%), Postives = 50/91 (54.95%), Query Frame = 0


Query: 29  STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRA 88
           +T V    V ++P P  +C YL+ S+  P   Y+G T D  RRL+QHNG  KGGAK T  
Sbjct: 2   TTTVSEEAVLTKPIPAFYCCYLLRSAKRPSALYIGSTPDPARRLEQHNGFAKGGAKRTER 61

Query: 89  G--RPWICACKIHGFKDQSQACEFESKWKEI 118
              RPW     + GF  ++ A +FE  W+ +
Sbjct: 62  NTLRPWEMVAIVEGFPSRTGALQFEWSWQHV 92

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy Swiss-Prot
Match: B6QFH5 (Structure-specific endonuclease subunit slx1 OS=Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333) OX=441960 GN=slx1 PE=3 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.3e-10
Identity = 44/136 (32.35%), Postives = 61/136 (44.85%), Query Frame = 0


Query: 33  PTLIVKSEPEPKKWCVYLIISSNPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRAG--R 92
           P  +   +P P  +C YL+ S   P   Y+G T D  RRL+QHNG  KGGAK T     R
Sbjct: 6   PADLPSPKPIPAFYCCYLLRSVKKPSSLYIGSTPDPARRLEQHNGFTKGGAKRTERDTLR 65

Query: 93  PWICACKIHGFKDQSQACEFESKWKEI----------CRKMCHMRKEEVDDQTLRLLKQR 152
           PW     I GF  ++ A +FE  W+ +            ++   RK+   DQ   +    
Sbjct: 66  PWEMITIIEGFTSRTGALQFEWSWQHVHTTRHIGAVETDQLNRRRKDPPVDQGSGIWTST 125

Query: 153 QRALGKVKGLFDCTQF 157
            + LG +  L   T F
Sbjct: 126 PKVLGNLHQLLRSTYF 141

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy Swiss-Prot
Match: Q4D7L5 (Structure-specific endonuclease subunit SLX1 homolog 1 OS=Trypanosoma cruzi (strain CL Brener) OX=353153 GN=Tc00.1047053509453.60 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.1e-09
Identity = 33/72 (45.83%), Postives = 44/72 (61.11%), Query Frame = 0


Query: 47  CVYLIISSNPPI--KTYVGVTLDFDRRLKQHNGEIKGGA-KATRAGRPWICACKIHGFKD 106
           CVYL+ S +P    + Y+G T+D  RRL+QHNGEI  GA +  R GRPW   C + GF +
Sbjct: 7   CVYLLTSLDPQCAGEYYIGYTVDPIRRLRQHNGEIVSGAWRTKRRGRPWELLCCVSGFGE 66

Query: 107 QSQACEFESKWK 116
              A +FE  W+
Sbjct: 67  DRIALKFEWCWQ 78

[Close]

BLAST of Cp4.1LG03g04710 vs. NCBI nr
Match: XP_023528241.1 (structure-specific endonuclease subunit slx1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337 bits (865), Expect = 6.45e-117
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MMRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKT 60
           MMRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKT
Sbjct: 1   MMRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKT 60

Query: 61  YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK 120
           YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK
Sbjct: 61  YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK 120

Query: 121 MCHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165
           MCHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF
Sbjct: 121 MCHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165

[Close]

BLAST of Cp4.1LG03g04710 vs. NCBI nr
Match: KAG6581557.1 (hypothetical protein SDJN03_21559, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 323 bits (829), Expect = 1.93e-111
Identity = 160/164 (97.56%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MMRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKT 60
           MMRLLSQTFRSQKLPISS EVSKSSSS STKVPTLIVKSEPEPKKWCVYLIISSNPPIKT
Sbjct: 1   MMRLLSQTFRSQKLPISSPEVSKSSSS-STKVPTLIVKSEPEPKKWCVYLIISSNPPIKT 60

Query: 61  YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK 120
           YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK
Sbjct: 61  YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK 120

Query: 121 MCHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 164
           MCH+RKEEVDD TLRLLKQRQRALGKVKGLFDCTQFEFDWELDP
Sbjct: 121 MCHLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 163

[Close]

BLAST of Cp4.1LG03g04710 vs. NCBI nr
Match: XP_022934184.1 (structure-specific endonuclease subunit slx1 [Cucurbita moschata])

HSP 1 Score: 323 bits (828), Expect = 2.64e-111
Identity = 160/163 (98.16%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61
           MRLLSQTFRSQKLPISS EVSKSSSS STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSS-STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 60

Query: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121
           VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM
Sbjct: 61  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 120

Query: 122 CHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 164
           CHMRKEEVDD TLRLLKQRQRALGKVKGLFDCTQFEFDWELDP
Sbjct: 121 CHMRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 162

[Close]

BLAST of Cp4.1LG03g04710 vs. NCBI nr
Match: KAG7018060.1 (slx1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 322 bits (825), Expect = 7.58e-111
Identity = 159/163 (97.55%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61
           MRLLSQTFRSQKLPISS EVSKSSSS STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSS-STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 60

Query: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121
           VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM
Sbjct: 61  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 120

Query: 122 CHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 164
           CH+RKEEVDD TLRLLKQRQRALGKVKGLFDCTQFEFDWELDP
Sbjct: 121 CHLRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 162

[Close]

BLAST of Cp4.1LG03g04710 vs. NCBI nr
Match: XP_022982643.1 (structure-specific endonuclease subunit slx1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 317 bits (811), Expect = 1.03e-108
Identity = 157/164 (95.73%), Postives = 159/164 (96.95%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61
           MRLLSQTFRSQKLPISS EVSKSSSS STKVPTL +KSEPE KKWCVYLIISSNPPIKTY
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSS-STKVPTLTIKSEPETKKWCVYLIISSNPPIKTY 60

Query: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121
           VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFK+QSQACEFESKWKEICRKM
Sbjct: 61  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKNQSQACEFESKWKEICRKM 120

Query: 122 CHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165
            HMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF
Sbjct: 121 SHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 163

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy TrEMBL
Match: A0A6J1F1V2 (structure-specific endonuclease subunit slx1 OS=Cucurbita moschata OX=3662 GN=LOC111441428 PE=4 SV=1)

HSP 1 Score: 323 bits (828), Expect = 1.28e-111
Identity = 160/163 (98.16%), Postives = 160/163 (98.16%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61
           MRLLSQTFRSQKLPISS EVSKSSSS STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSS-STKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 60

Query: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121
           VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM
Sbjct: 61  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 120

Query: 122 CHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 164
           CHMRKEEVDD TLRLLKQRQRALGKVKGLFDCTQFEFDWELDP
Sbjct: 121 CHMRKEEVDDVTLRLLKQRQRALGKVKGLFDCTQFEFDWELDP 162

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy TrEMBL
Match: A0A6J1J5C9 (structure-specific endonuclease subunit slx1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481456 PE=4 SV=1)

HSP 1 Score: 317 bits (811), Expect = 5.01e-109
Identity = 157/164 (95.73%), Postives = 159/164 (96.95%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61
           MRLLSQTFRSQKLPISS EVSKSSSS STKVPTL +KSEPE KKWCVYLIISSNPPIKTY
Sbjct: 1   MRLLSQTFRSQKLPISSPEVSKSSSS-STKVPTLTIKSEPETKKWCVYLIISSNPPIKTY 60

Query: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121
           VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFK+QSQACEFESKWKEICRKM
Sbjct: 61  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKNQSQACEFESKWKEICRKM 120

Query: 122 CHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165
            HMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF
Sbjct: 121 SHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 163

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy TrEMBL
Match: A0A0A0LCZ9 (GIY-YIG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683170 PE=4 SV=1)

HSP 1 Score: 262 bits (669), Expect = 2.82e-87
Identity = 135/171 (78.95%), Postives = 149/171 (87.13%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPK--KWCVYLIISSNPPIK 61
           MRLLS TFR  K PIS+  +SKSSSS+S K PTLIVKSEP+PK  +WCVYLIISSN PIK
Sbjct: 1   MRLLSHTFRCVKPPISNPPLSKSSSSSS-KDPTLIVKSEPKPKLKQWCVYLIISSNSPIK 60

Query: 62  TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICR 121
           TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICAC IHGFKDQSQACEFESKWK++ R
Sbjct: 61  TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACTIHGFKDQSQACEFESKWKKVSR 120

Query: 122 KMCHMRKEE-----VDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165
           K+ + +KEE     +DDQTLRLLK R+RALGKVK LFDC+QFEFDW+LDPF
Sbjct: 121 KISYEKKEEDVGKLLDDQTLRLLKHRERALGKVKCLFDCSQFEFDWKLDPF 170

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy TrEMBL
Match: A0A1S3CFH5 (structure-specific endonuclease subunit slx1 OS=Cucumis melo OX=3656 GN=LOC103500344 PE=4 SV=1)

HSP 1 Score: 260 bits (664), Expect = 1.57e-86
Identity = 133/170 (78.24%), Postives = 148/170 (87.06%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVK-SEPEPKKWCVYLIISSNPPIKT 61
           MRLLSQTFR QK PIS+  + KSSSS STK PTLIVK  EP+PK+WCVYLIISSN PIKT
Sbjct: 1   MRLLSQTFRCQKPPISNPRLPKSSSS-STKGPTLIVKLEEPKPKQWCVYLIISSNSPIKT 60

Query: 62  YVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRK 121
           YVGVTLDFDRRLKQHNGEIKGGAKAT+AGRPWICAC IHGFKDQSQACEFESKWK++ RK
Sbjct: 61  YVGVTLDFDRRLKQHNGEIKGGAKATQAGRPWICACTIHGFKDQSQACEFESKWKKVSRK 120

Query: 122 MCHMRKEE-----VDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165
           + + +KE+     +D+QTLRLLK RQRALGKVK LFDC+ FEFDW+LDPF
Sbjct: 121 ISYEKKEDDVGKQLDNQTLRLLKHRQRALGKVKCLFDCSHFEFDWKLDPF 169

[Close]

BLAST of Cp4.1LG03g04710 vs. ExPASy TrEMBL
Match: A0A6J1DGL6 (structure-specific endonuclease subunit SLX1 OS=Momordica charantia OX=3673 GN=LOC111020266 PE=4 SV=1)

HSP 1 Score: 236 bits (603), Expect = 8.92e-77
Identity = 118/169 (69.82%), Postives = 134/169 (79.29%), Query Frame = 0


Query: 2   MRLLSQTFRSQKLPISSSEVSKSSSSASTKVPTLIVKSEPEPKKWCVYLIISSNPPIKTY 61
           MRLLS TFR  K PIS+ + S SSS+      TL  +S+ +PK WCVYLIISSN P KTY
Sbjct: 33  MRLLSHTFRCHKSPISNPKPSSSSSTKDPSAATLSFESQLKPKPWCVYLIISSNSPFKTY 92

Query: 62  VGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFESKWKEICRKM 121
           VGVTL+F RRLKQHNGEIKGGAKATRAGRPW+CAC IHGFKDQSQACEFESKWKE+ RK+
Sbjct: 93  VGVTLNFSRRLKQHNGEIKGGAKATRAGRPWVCACMIHGFKDQSQACEFESKWKEVSRKI 152

Query: 122 CHMRK-----EEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWELDPF 165
            H RK     E+ D+Q LRLLK RQRAL KVK +FDC++FEFD +LDPF
Sbjct: 153 AHKRKKDDEEEQADEQKLRLLKNRQRALEKVKFMFDCSRFEFDGQLDPF 201

[Close]

BLAST of Cp4.1LG03g04710 vs. TAIR 10
Match: AT5G43210.1 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 156.8 bits (395), Expect = 1.5e-38
Identity = 87/170 (51.18%), Postives = 111/170 (65.29%), Query Frame = 0


Query: 1   MMRLLSQTFRSQKLPISSSEVS--KSSSSASTKVPTLIVKSE------PEPKKWCVYLII 60
           M RLLS+TF S KL  S+ +    K+  ++ T+VP     S       P+ K W VYLI+
Sbjct: 1   MTRLLSRTFPSVKLRDSNPKAPEFKNLKNSQTQVPIPSSSSPQENSKIPKSKSWSVYLIL 60

Query: 61  SSNPPIKTYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQACEFES 120
           S+  PIKTYVG+T DF RRLKQHNGEI+GGAKA+ AGRPW+CAC I GF   SQA  FES
Sbjct: 61  STTEPIKTYVGITTDFSRRLKQHNGEIRGGAKASSAGRPWLCACIITGFTCLSQASSFES 120

Query: 121 KWKEICRKMCHMRKEEVDDQTLRLLKQRQRALGKVKGLFDCTQFEFDWEL 163
           KWK   RK+   +K+E   Q+  LL+ R+RAL KV+   DC+  E DW++
Sbjct: 121 KWKIFTRKLPRRKKDEDMSQSDALLQHRRRALNKVEESLDCSYLETDWKI 170

[Close]

BLAST of Cp4.1LG03g04710 vs. TAIR 10
Match: AT2G30350.2 (Excinuclease ABC, C subunit, N-terminal )

HSP 1 Score: 60.5 bits (145), Expect = 1.5e-09
Identity = 30/69 (43.48%), Postives = 41/69 (59.42%), Query Frame = 0


Query: 49  YLIISSNPPIK--TYVGVTLDFDRRLKQHNGEIKGGAKATRAGRPWICACKIHGFKDQSQ 108
           YL+ S +P  K  TY+G T++  RR++QHNGEI  GA  T+  RPW     I+GF     
Sbjct: 31  YLLTSLSPRHKGQTYIGFTVNPRRRIRQHNGEITSGAWRTKKKRPWEMVLCIYGFPTNVS 90

Query: 109 ACEFESKWK 116
           A +FE  W+
Sbjct: 91  ALQFEWAWQ 99

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g04710 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B6JY16	6.4e-13	38.39	Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces japonicus (s...	[more]
Q9P7M3	9.2e-12	43.24	Structure-specific endonuclease subunit slx1 OS=Schizosaccharomyces pombe (strai...	[more]
B8MDD1	6.0e-11	39.56	Structure-specific endonuclease subunit slx1 OS=Talaromyces stipitatus (strain A...	[more]
B6QFH5	1.3e-10	32.35	Structure-specific endonuclease subunit slx1 OS=Talaromyces marneffei (strain AT...	[more]
Q4D7L5	1.1e-09	45.83	Structure-specific endonuclease subunit SLX1 homolog 1 OS=Trypanosoma cruzi (str...	[more]

BLAST of Cp4.1LG03g04710 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528241.1	6.45e-117	100.00	structure-specific endonuclease subunit slx1 [Cucurbita pepo subsp. pepo]	[more]
KAG6581557.1	1.93e-111	97.56	hypothetical protein SDJN03_21559, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022934184.1	2.64e-111	98.16	structure-specific endonuclease subunit slx1 [Cucurbita moschata]	[more]
KAG7018060.1	7.58e-111	97.55	slx1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022982643.1	1.03e-108	95.73	structure-specific endonuclease subunit slx1 isoform X1 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG03g04710 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1V2	1.28e-111	98.16	structure-specific endonuclease subunit slx1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1J5C9	5.01e-109	95.73	structure-specific endonuclease subunit slx1 isoform X1 OS=Cucurbita maxima OX=3...	[more]
A0A0A0LCZ9	2.82e-87	78.95	GIY-YIG domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G683170 PE=...	[more]
A0A1S3CFH5	1.57e-86	78.24	structure-specific endonuclease subunit slx1 OS=Cucumis melo OX=3656 GN=LOC10350...	[more]
A0A6J1DGL6	8.92e-77	69.82	structure-specific endonuclease subunit SLX1 OS=Momordica charantia OX=3673 GN=L...	[more]

BLAST of Cp4.1LG03g04710 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43210.1	1.5e-38	51.18	Excinuclease ABC, C subunit, N-terminal	[more]
AT2G30350.2	1.5e-09	43.48	Excinuclease ABC, C subunit, N-terminal	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035901	GIY-YIG endonuclease superfamily	GENE3D	3.40.1440.10		coord: 40..144 e-value: 2.8E-23 score: 83.8
IPR035901	GIY-YIG endonuclease superfamily	SUPERFAMILY	82771	GIY-YIG endonuclease	coord: 45..121
IPR000305	GIY-YIG endonuclease	PFAM	PF01541	GIY-YIG	coord: 45..116 e-value: 4.8E-14 score: 52.4
IPR000305	GIY-YIG endonuclease	PROSITE	PS50164	GIY_YIG	coord: 44..129 score: 17.241772
None	No IPR available	PANTHER	PTHR20208:SF13	EMB\|CAB76036.1	coord: 15..164
None	No IPR available	PANTHER	PTHR20208	STRUCTURE-SPECIFIC ENDONUCLEASE SUBUNIT SLX1	coord: 15..164
None	No IPR available	CDD	cd10455	GIY-YIG_SLX1	coord: 46..115 e-value: 3.71305E-35 score: 115.793

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g04710.1	Cp4.1LG03g04710.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0033557	Slx1-Slx4 complex
molecular_function	GO:0017108	5'-flap endonuclease activity
molecular_function	GO:0008821	crossover junction endodeoxyribonuclease activity