Cp4.1LG03g04570 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g04570
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	tRNA-dihydrouridine synthase
Location	Cp4.1LG03: 5398146 .. 5405740 (+)
RNA-Seq Expression	Cp4.1LG03g04570
Synteny	Cp4.1LG03g04570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAGTTTGCTGGATGTTCTGTTTCAGCTTCTTTGTCTCCCGTCCAGTCTTTCATTCAGAAGAGCCATCCTAGGCTGTCATCCAGTATATTGCAAAAGAATTTGTATAAACCAAGTAGTTGGTTTTCTGGTAATCAGATACTTCAGCAAAGTCCAGAAGCAGAAATGGTTGCTGGACGTTATTCCCCTCCTTGGTTTAGGTGAGTTTTCCGCATCATATAAATGTTGTTATTGTCATCATTTGTGTTCCTTGAGCGTTTATATCTGATGACCTTGATTGACTTTTATTTACTTTCTCAGTGTTGCTCCTATGATGGATTGCACTGACAATCACTACAGAACTCTTGCTCGTTTAATATCAAAGCACGCATGGCTGTACAGCGAGATGCTTGCTGCTGAAACAATTGTTTACCAAAAAGATAATCTTGTAAGTTTGCGGCAGAACAGAATCCTTGGACAATAATTAATTTATCAACGTTGTCTACAAATTTACTAAGTAGTGTTGCTTTTATTTATTAACAATCATTTTTAGAGGCAGATGCAATTTCCTTGATTTTCCATTATTTACTTGAGGTTTTTTAAATTGTGTAACACGTTGGGTTAGTTATCAATTATTTGTGATAACCCTCCCCTCACCCTTGCAAGCGCATTGCTTCCATGTGAATGGGTCGGGTTAGTGAATAATCAATTGTTCCCTCTTTCCCCACTCTTATGAGAAGCTTTTCCTCCAGTTGCCTTAACAAAACTTCCTGGCCCTGAATCCTTCAACATCCCACAGCTTTCACGTGTGATAGTCTGAGTTGGACCATGGTTTTCTCTCATTTTACCAACATTTGGTCAGCAATAGTGACGGTGTGGTGGTCTTTTAAAATTATGGGATGCTCTAATCAGATTGAACATTTTAACAGTAGACTACTATTTTTTTTACCTTTCATTGTGTGAATCAGCTTAGTTTCCAAATTAGCTTGACCTCATATCCTGTTTGTTTCATTATCAGCCAAGTTGGACTTCACTGTTGACTAATTTATGATCGGTAAGAGTGAAGAATTATTGGGTGTTTTATTTTTCTCAAAGAAGAGTTGCCACTTACCATCTTATTGCTACCTTTGTAACTTGCATATTTTATCTGGTTGGTGTTGGAGGCACGTGACAATTTCCCTATCTTTTCATGATCAAACTTGAGATGCATATGATATGCTTCAACAGNTTTTAGAGGCAGATGCAATTTCCTTGATTTTCCATTATTTACTCGAGGTTTTTTAAATTGTGTAACACATTGGGTTAGTTATCAATTATTTGTGATAACCCTCCCCTCACCCTTGTAAGCGCATTGCTTCCATGTGAATGGGTCGGGTTAGTGAATAATCAATTGTTCCCTCTTTCCCCACTCTTATGAGAAGCTTTTCCTCCAGTTGCCTTAACAAAACTTCCTGGCCCTGAATCCTTCAACATCCCACAGCTTTCACGTGTGATAGTCTGAGTTGGACCATGGTTTTCTCTCATTTTACCAACATTTGGTCAGCAATAGTGACGGTGTGGTGGTCTTTTAAAATTATGGGATGCTCTAATCAGATTGAACATTTTAACAGTAGACTACTATTTTTTTTACCTTTCATTGTGTGAATCAGCTTAGTTTCCAAATTAGCTTGACCTCATATCCTGTTTGTTTCATTATCAGCCAAGTTGGACTTCACTGTTGACTAATTTATGATCGGTAAGAGTGAAGAATTATTGGGTGTTTTATTTTTCTCAAAGAAGAGTTGCCACTTACCATCTTATTGCTACCTTTGTAACTTGCATATTTTATCTGGTTGGTGTTGGAGGCACGTGACAATTTCCCTATCTTTTCATGATCAAACTTGAGATGCATATGATATGCTTCAACAGTCTATGTTTTGACCATTTTTTCATGTAATCTTTCTTTATTTCGTACAGGACAGANAGTCTATGTTTTGACCATTTTTTCATGTAATCTTTCTTTATTTTGTACAGGACAGGNTATTTCGTACAGGACAGATTTTTAGCATTTTCTCCAGACCAGCATCCTATTGTCCTGCAAATTGGAGGAAACAATTTGAATAACATTGCAAAAGCTGTTGAACTTGCCAATCCTTACGGCTATGATGAAATTAATTTGAAGTGAGTATTTTACTTATCTTCTTCATATTTTTCCAGTTTTAGTTTCTACAATAGATTCTTATCTCTATAATTTTTGGAAACTATTTTTCAGCTGTGGATGTCCAAGTCCAAAGGTGGCTGGACATGGGTGCTTCGGTGCTCGCCTTATGCTGGATCCTAAGGTTTCTGGCTGTATACTGGTTATATTTTGATTTCAGTCATCTTGCCCATTTTGTTTTCTTCAGCTATGTGTCTAACTTATGCTTGTCATGATGACAAGGAAAGTTTGTTGGTGAAGCTATGTCAGTAATTGCTGCCAACACTGACGCCCCTGTAAGCGTGAAATGTCGAATTGGCGTCGATGACCATGATTCATATAATGAACTTTGTAAGGTTTCTAGTCTTTTTTCTCTGATTAGAAAGCACTGACTACTACTTCTTTGTTTAGATAAACATAGTGATGTTGTAAGGTTAGTTTTGCCATTTTATGATTAGCATCTTTATTTATTTCCCCTGTGGAGCATCATCACAATAATTGACTGTTTGATATTTTGTGTCCTTTCCTGTCTGAATATCTTTCTTGTGCTTAAAGGTGGCTTTGATTACACATTGAAGTGAATGAACTGGTAACATTATAAGTTATAGATATGACCTCAAAGGGAATGCTATCTCATTAGGAACTCTTAATATAATCTTTGAGAGTGAAAGTTATCTGTCTTGGAGCAGCTTTTGTTAAGCTCCTCAGGCTGTGATTCCTTGTTCCTTTATTTATTTATTATTATTATTTTTTTTACTTTTTCACCCTATGAGTAAGAAATCTAGTTTGTTTTTTAAAAAAGAAAAAGAAAAGGAAGAAGACTTTTACCACCCACAACTTCATTTTTTTTCCATTTCATGAAAGACTAACTTTTTATACTTGGTAGAGAAAAACATATGAAGGCAAAGAAAAACTTCTTTGGAGAAACTCCTTTGGTATACAGTCATAAACCATCTTAGATCCTGAATTGTGCTAGGTCAACTCAAGCACAGGATATCTAATTTATTCTTGGTGTTTGAAATTTGAAGTTTTCAGCATATATATTGTCATTATGTGCTAACAATTTCAAGAGGCCCTGAGGTTTTGAATATTCTTTCTGAAAAGCCATTCTAGTTTCTTGTTACATTATTATGTGCCTTCTTAGTTGCTGATTTCTCCTCTCACGTCCACTTTCTTTTGTTTCAGGTGATTTTATTTACAAAGTTTCTTCTTTGTCGCCAACTAAGCATTTCATAATCCATTCCCGCAAGGCACTGCTCAATGGTATTAGCCCAGCTGAAAATCGAAATATTCCTCCTTTGAAGTATGGGTTCTCAAGAACATTCTATCAGCTTAAAGAGAGCTTGTTGTTTTTTGTTAAAGTATTTTAACAAAGACCATATAATATTTTTAACTATCTGTAAAATAATGACTAAGAACAGACTTTCATGCTCTATAATACTGACGTGGTCACAGATATGAATATTTCTATGCACTGATGCGCGACTTTCCAGACTTGAGATTCACAATAAATGGGGGCATTAACACTGTTGATGAGGTGAGTTTTTCTGCTCATCTGTAAATGGACCTAATGTATCAGAAATTGGATTTACCCAAACTAGAAAAGCATACTAATAGATATTGAAATCACGGCAAAATAGGGGCTAGCCTAGTAGATTATATAGCTTTTTACATGACGTGGTCTCCACTGATGCAACAAAGGCATAGTGGAAACATTCATATTTTTCTATGTCTTGCATTGCATTATCATTTTGCAATGACCAATTTACACGGTTTATTGACCCAAAACTCGTTTATTGGGAGTTGCTTTCCATGGAATGAAAGTTCCTTGTGGTAAATTGGCAGCATACGTTAATGTTTATTTACTTTAAATGTGATAAATTGCTTTCCATGGAGTGAGAGTTCCATGCATTATCATAAATTAATGTTCATTTATTTTAAATGTGTTCACACAGGTTAATGCTGCTCTCAGGCTCGGAGCTCATGGTGTAATGGTGGGTCGAGCTGCTTACCAAAAGTATGTTATGCACATTTTGGAAATTACCATCTTTTCCCTCTTTCGTTTGCTTGTATCACAAATAAGTATAGGCAACTTCTCGCATTCTTCTCAAGGTATAAGATAATTCTATAGTCACAGCTAGCTCCAACCATTACCAGTATAAGAGAACCTGAAGTTTGAGCGACAATTGGGTTCCATGTTTAAAAATTAGAATTCTCCTATAATATCTGATTATATCTGTCTGTGGAGGCCGGTTTAAGGTGATGTTATTATAGTGAAAAATAAAAGTGCTAGAACTTATGGCATCATAGAGTGTTCTTTTTATTTTTTAAATATAGACATTTTAAATATATTTATTTAACTTATTAAAATTGAAGTTAAGTGTGATGTTATTAGTTGGGTTAAGTATTTATTTGTATTTTCCAACAATATCTTGGTATGAACATTAAGTATTTATTCTATCAGAGTTGGTTATGTCTTTCCTTAATTTTAACTATGGCTGTTATACTTCATAGCCCTTGGCGTACTTTAGGACATGTAGACACTGCAATTTATGGTGCGCCTAGCAGTGGTATAACCCGTCGTCAGGTACAATATTAAAGTTGGCTGTGAAATGTATCCTTCTGCTTGAAAGTTCACTATTATCTCATTTAGCTGTAAACTTTTCCATCTCCTAGGTCCTTGAACAATATCAAGTTTATGGGGATTCTGTTTTGGGTACATATGGAAATAAACCAAATATTCGTGATGTTGTGAAGGTAAGATCTTATTGGTGGTTTACAGCCTTTTTGTTAATTTTTTTTAAACCACAATTTTTGTCTCCTATGTTTTCAGTTGGTCTTGTTTCTTTTATATGCCACATATTTCTTAACTATATTTTTTCGATTGTGGGTTGGGGTTCTGAACATTTTTATGGGGTTGGGTTTAATTTAACTCTACTTATTTTCAGTAATTTTAAATGTGTGACATGAACTCTGTATTCTTCCCTCACTATCTTGTGCCATATGTGGGATTAGGGAGACCGAGCGCTATTAAGGGAGTAGGACTTATGATTATTAAGGTGTTGGAATAGCATTTGTGCACTAGTTAGACCGTGTGGATTCCATGGTCAAATATTTGCACTGTATAATGTTATTATTGCATTTTGGATCTCTAGGTTGGGCGTAAATGCATGATGAGTCCCACTAGATTGATTAAACTGAGGTCCCTTTAAGGTTATTTCCATTATTGCATTTTTGTAACTTGGAGCCCTCCCTGATTACTGCCAATTGGAGGAAACTCTGTATTTCCGTGGGTTAGCCATTTCATACATCAACGATGGCTGAGCATGCTGAATTATCGTCTTCTCTTGCTTCTACATCTTCGTTTCACTGATAATTTTTGTCCTTTTATTGCCTTATACCAATCTAGGACCTNTATAGTCACAGCTAGCTCCAACCATTACCAGTATAAGAGAACCTGAAGTTTGAGCGACAATTGGGTTCCATGTTTAAAAATTAGAATTCTCCTATAATATCTGATTATATCTGTCTGTGGAGGCCGGTTTAAGGTGATGTTATTATAGTGAAAAATAAAAGTGCTAGAACTTATGGCATCATAGAGTGTTCTTTTTATTTTTTAAATATAGACATTTTAAATATATTTATTTAACTTATTAAAATTGAAGTTAAGTGTGATGTTATTAGTTGGGTTAAGTATTTATTTGTATTTTCCAACAATATCTTGGTATGAACATTAAGTATTTATTCTATCAGAGTTGGTTATGTCTTTCCTTAATTTTAACTATGGCTGTTATACTTCATAGCCCTTGGCGTACTTTAGGACATGTAGACACTGCAATTTATGGTGCGCCTAGCAGTGGTATAACCCGTCGTCAGGTACAATATTAAAGTTGGCTGTGAAATGTATCCTTCTGCTTGAAAGTTCACTATTATCTCATTTAGCTGTAAACTTTTCCATCTCCTAGGTCCTTGAACAATATCAAGTTTATGGGGATTCTGTTTTGGGTACATATGGAAATAAACCAAATATTCGTGATGTTGTGAAGGTAAGATCTTATTGGTGGTTTACAGCCTTTTTGTTAATTTTTTTTAAACCACAATTTTTGTCTCCTATGTTTTCAGTTGGTCTTGTTTCTTTTATATGCCACATATTTCTTAACTATATTTTTTCGATTGTGGGTTGGGGTTCTGAACATTTTTATGGGGTTGGGTTTAATTTAACTCTACTTATTTTCAGTAATTTTAAATGTGTGACATGAACTCTGTATTCTTCCCTCACTATCTTGTGCCATATGTGGGATTAGGGAGACCGAGCGCTATTAAGGGAGTAGGACTTATGATTATTAAGGTGTTGGAATAGCATTTGTGCACTAGTTAGACCGTGTGGATTCCATGGTCAAATATTTGCACTGTATAATGTTATTATTGCATTTTGGATCTCTAGGTTGGGCGTAAATGCATGATGAGTCCCACTAGATTGATTAAACTGAGGTCCCTTTAAGGTTATTTCCATTATTGCATTTTTGTAACTTGGAGCCCTCCCTGATTACTGCCAATTGGAGGAAACTCTGTATTTCCGTGGGTTAGCCATTTCATACATCAACGATGGCTGAGCATGCTGAATTATCGTCTTCTCTTGCTTCTACATCTTCGTTTCACTGATAATTTTTGTCCTTTTATTGCCTTATACCAATCTAGGACCTCTTTTATCCGTCATGTCTTATAAGTTGCTATTTTTTTTTTTCTTAATCAATACTGATTGGAATCTAGCAAAACGCACCCCCACCCCCATACAATACCTGGTGTGGTCATTTTACATTTCTCCTTTATCTGCTTCTGCAGCCGTTGCTTCACCTTTTTTATACAGATCCTGGAAATGGTCCATGGAAGCGTAAAGCTGATTTTGCTTTTCAGCATTGCAAGGTATAAAAATATGCTGGAAGAGAATAGGTTCAACTTAATATCGTTTCGTAGATTTCGATGGAATGCATGGCGTATTTTTCCTCTGCTTCCTTTCAACAGTTTCATAGGATTTTTTATTTTGATTTTAGTTTTACAAAAATTTGGAAATTCAAATCGGATATTCAAATTTCATGTTTCTATCACCGAGATTTTTCACTGCCATTGTTATCGAGGTTGTCTGTCCATCAAGTATCCCTACCTTGTAAATGCTTTNCATTGTTATCGAGGTTGTCTGTCCATCAAGTATCCCTACCTTGCAAATGCTTGGCTGCATAGCTTCTAAGTGAACTGCTATTGAACCTTGTAGACCATCAAATCATTTTTCGAGGAAACACTTGTGGCCATTCCAGATTATATATTGGATGCTCCTGTAGCAGAACCTCCATCAGGACGTGAAGATCTTTTTGCCAATACGCTTAGATTGATGCCTCCTCCATACGAGGACAGAGAGCAAAAGGTAGTATTAGAAGCTTAA

mRNA sequence

ATGATGAAGTTTGCTGGATGTTCTGTTTCAGCTTCTTTGTCTCCCGTCCAGTCTTTCATTCAGAAGAGCCATCCTAGGCTGTCATCCAGTATATTGCAAAAGAATTTGTATAAACCAAGTAGTTGGTTTTCTGGTAATCAGATACTTCAGCAAAGTCCAGAAGCAGAAATGGTTGCTGGACGTTATTCCCCTCCTTGGTTTAGTGTTGCTCCTATGATGGATTGCACTGACAATCACTACAGAACTCTTGCTCGTTTAATATCAAAGCACGCATGGCTGTACAGCGAGATGCTTGCTGCTGAAACAATTGTTTACCAAAAAGATAATCTTGACAGATTTTTAGCATTTTCTCCAGACCAGCATCCTATTGTCCTGCAAATTGGAGGAAACAATTTGAATAACATTGCAAAAGCTGTTGAACTTGCCAATCCTTACGGCTATGATGAAATTAATTTGAACTGTGGATGTCCAAGTCCAAAGGTGGCTGGACATGGGTGCTTCGGTGCTCGCCTTATGCTGGATCCTAAGTTTGTTGGTGAAGCTATGTCAGTAATTGCTGCCAACACTGACGCCCCTGTAAGCGTGAAATGTCGAATTGGCGTCGATGACCATGATTCATATAATGAACTTTGTGATTTTATTTACAAAGTTTCTTCTTTGTCGCCAACTAAGCATTTCATAATCCATTCCCGCAAGGCACTGCTCAATGGTATTAGCCCAGCTGAAAATCGAAATATTCCTCCTTTGAAATATGAATATTTCTATGCACTGATGCGCGACTTTCCAGACTTGAGATTCACAATAAATGGGGGCATTAACACTGTTGATGAGGTTAATGCTGCTCTCAGGCTCGGAGCTCATGGTGTAATGGTGGGTCGAGCTGCTTACCAAAACCCTTGGCGTACTTTAGGACATGTAGACACTGCAATTTATGGTGCGCCTAGCAGTGGTATAACCCGTCGTCAGGTCCTTGAACAATATCAAGTTTATGGGGATTCTGTTTTGGGTACATATGGAAATAAACCAAATATTCGTGATGTTGTGAAGAGTTGGTTATGTCTTTCCTTAATTTTAACTATGGCTGTTATACTTCATAGCCCTTGGCGTACTTTAGGACATGTAGACACTGCAATTTATGGTGCGCCTAGCAGTGGTATAACCCGTCGTCAGGTCCTTGAACAATATCAAGTTTATGGGGATTCTGTTTTGGGTACATATGGAAATAAACCAAATATTCGTGATGTTGTGAAGCCGTTGCTTCACCTTTTTTATACAGATCCTGGAAATGGTCCATGGAAGCGTAAAGCTGATTTTGCTTTTCAGCATTGCAAGACCATCAAATCATTTTTCGAGGAAACACTTGTGGCCATTCCAGATTATATATTGGATGCTCCTGTAGCAGAACCTCCATCAGGACGTGAAGATCTTTTTGCCAATACGCTTAGATTGATGCCTCCTCCATACGAGGACAGAGAGCAAAAGGTAGTATTAGAAGCTTAA

Coding sequence (CDS)

ATGATGAAGTTTGCTGGATGTTCTGTTTCAGCTTCTTTGTCTCCCGTCCAGTCTTTCATTCAGAAGAGCCATCCTAGGCTGTCATCCAGTATATTGCAAAAGAATTTGTATAAACCAAGTAGTTGGTTTTCTGGTAATCAGATACTTCAGCAAAGTCCAGAAGCAGAAATGGTTGCTGGACGTTATTCCCCTCCTTGGTTTAGTGTTGCTCCTATGATGGATTGCACTGACAATCACTACAGAACTCTTGCTCGTTTAATATCAAAGCACGCATGGCTGTACAGCGAGATGCTTGCTGCTGAAACAATTGTTTACCAAAAAGATAATCTTGACAGATTTTTAGCATTTTCTCCAGACCAGCATCCTATTGTCCTGCAAATTGGAGGAAACAATTTGAATAACATTGCAAAAGCTGTTGAACTTGCCAATCCTTACGGCTATGATGAAATTAATTTGAACTGTGGATGTCCAAGTCCAAAGGTGGCTGGACATGGGTGCTTCGGTGCTCGCCTTATGCTGGATCCTAAGTTTGTTGGTGAAGCTATGTCAGTAATTGCTGCCAACACTGACGCCCCTGTAAGCGTGAAATGTCGAATTGGCGTCGATGACCATGATTCATATAATGAACTTTGTGATTTTATTTACAAAGTTTCTTCTTTGTCGCCAACTAAGCATTTCATAATCCATTCCCGCAAGGCACTGCTCAATGGTATTAGCCCAGCTGAAAATCGAAATATTCCTCCTTTGAAATATGAATATTTCTATGCACTGATGCGCGACTTTCCAGACTTGAGATTCACAATAAATGGGGGCATTAACACTGTTGATGAGGTTAATGCTGCTCTCAGGCTCGGAGCTCATGGTGTAATGGTGGGTCGAGCTGCTTACCAAAACCCTTGGCGTACTTTAGGACATGTAGACACTGCAATTTATGGTGCGCCTAGCAGTGGTATAACCCGTCGTCAGGTCCTTGAACAATATCAAGTTTATGGGGATTCTGTTTTGGGTACATATGGAAATAAACCAAATATTCGTGATGTTGTGAAGAGTTGGTTATGTCTTTCCTTAATTTTAACTATGGCTGTTATACTTCATAGCCCTTGGCGTACTTTAGGACATGTAGACACTGCAATTTATGGTGCGCCTAGCAGTGGTATAACCCGTCGTCAGGTCCTTGAACAATATCAAGTTTATGGGGATTCTGTTTTGGGTACATATGGAAATAAACCAAATATTCGTGATGTTGTGAAGCCGTTGCTTCACCTTTTTTATACAGATCCTGGAAATGGTCCATGGAAGCGTAAAGCTGATTTTGCTTTTCAGCATTGCAAGACCATCAAATCATTTTTCGAGGAAACACTTGTGGCCATTCCAGATTATATATTGGATGCTCCTGTAGCAGAACCTCCATCAGGACGTGAAGATCTTTTTGCCAATACGCTTAGATTGATGCCTCCTCCATACGAGGACAGAGAGCAAAAGGTAGTATTAGAAGCTTAA

Protein sequence

MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAGRYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGEAMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTMAVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLLHLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANTLRLMPPPYEDREQKVVLEA

Homology

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy Swiss-Prot
Match: Q8X5V6 (tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O157:H7 OX=83334 GN=dusA PE=3 SV=3)

HSP 1 Score: 234.2 bits (596), Expect = 3.2e-60
Identity = 131/299 (43.81%), Postives = 179/299 (59.87%), Query Frame = 0


Query: 45  GNQILQQSPEAEMVAGRYSPPW---FSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAE 104
           GN  +Q+  +   +  +    W   FSVAPM+D TD H R   RL+S++  LY+EM+   
Sbjct: 3   GNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTG 62

Query: 105 TIVYQKDNLDRFLAFSPDQHPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKV 164
            I++ K +   +LA+S ++HP+ LQ+GG++   +A+  +LA   GYDEINLN GCPS +V
Sbjct: 63  AIIHGKGD---YLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRV 122

Query: 165 AGHGCFGARLMLDPKFVGEAMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLS 224
             +G FGA LM + + V + +  +      PV+VK RIG+DD DSY  LCDFI  VS   
Sbjct: 123 Q-NGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKG 182

Query: 225 PTKHFIIHSRKALLNGISPAENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAA 284
             + FIIH+RKA L+G+SP ENR IPPL Y   Y L RDFP L  +INGGI +++E  A 
Sbjct: 183 ECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH 242

Query: 285 LRLGAHGVMVGRAAYQNPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTY 339
           L+    GVMVGR AYQNP   L  VD  I+G+  +      V+     Y +  L  GTY
Sbjct: 243 LQ-HMDGVMVGREAYQNP-GILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTY 295

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy Swiss-Prot
Match: P32695 (tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli (strain K12) OX=83333 GN=dusA PE=1 SV=4)

HSP 1 Score: 234.2 bits (596), Expect = 3.2e-60
Identity = 131/299 (43.81%), Postives = 179/299 (59.87%), Query Frame = 0


Query: 45  GNQILQQSPEAEMVAGRYSPPW---FSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAE 104
           GN  +Q+  +   +  +    W   FSVAPM+D TD H R   RL+S++  LY+EM+   
Sbjct: 3   GNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTG 62

Query: 105 TIVYQKDNLDRFLAFSPDQHPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKV 164
            I++ K +   +LA+S ++HP+ LQ+GG++   +A+  +LA   GYDEINLN GCPS +V
Sbjct: 63  AIIHGKGD---YLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRV 122

Query: 165 AGHGCFGARLMLDPKFVGEAMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLS 224
             +G FGA LM + + V + +  +      PV+VK RIG+DD DSY  LCDFI  VS   
Sbjct: 123 Q-NGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKG 182

Query: 225 PTKHFIIHSRKALLNGISPAENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAA 284
             + FIIH+RKA L+G+SP ENR IPPL Y   Y L RDFP L  +INGGI +++E  A 
Sbjct: 183 ECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAH 242

Query: 285 LRLGAHGVMVGRAAYQNPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTY 339
           L+    GVMVGR AYQNP   L  VD  I+G+  +      V+     Y +  L  GTY
Sbjct: 243 LQ-HMDGVMVGREAYQNP-GILAAVDREIFGSSDTDADPVAVVRAMYPYIERELSQGTY 295

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy Swiss-Prot
Match: Q7UBC5 (tRNA-dihydrouridine(20/20a) synthase OS=Shigella flexneri OX=623 GN=dusA PE=3 SV=3)

HSP 1 Score: 231.9 bits (590), Expect = 1.6e-59
Identity = 127/274 (46.35%), Postives = 170/274 (62.04%), Query Frame = 0


Query: 67  FSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQ 126
           FSVAPM+D TD H R   RL+S++  LY+EM+    I++ K +   +LA+S ++HP+ LQ
Sbjct: 22  FSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGD---YLAYSEEEHPVALQ 81

Query: 127 IGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGEAMSVIA 186
           +GG++   +A+  +LA   GYDEINLN GCPS +V  +G FGA LM + + V + +  + 
Sbjct: 82  LGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ-NGMFGACLMGNAQLVADCVKAMR 141

Query: 187 ANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENRNI 246
                PV+VK RIG+DD DSY  LCDFI  VS     + FIIH+RKA L+G+SP ENR I
Sbjct: 142 DVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREI 201

Query: 247 PPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPWRTLGHV 306
           PPL Y   Y L RDFP L  +INGGI +++E  A L+    GVMVGR AYQNP   L  V
Sbjct: 202 PPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ-HMDGVMVGREAYQNP-GILAAV 261

Query: 307 DTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTY 339
           D  I+G+  +      V+     Y +  L  GTY
Sbjct: 262 DREIFGSSDTDADPVAVVRAMYPYIEHELSQGTY 289

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy Swiss-Prot
Match: Q8FB30 (tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) OX=199310 GN=dusA PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.1e-59
Identity = 126/274 (45.99%), Postives = 170/274 (62.04%), Query Frame = 0


Query: 67  FSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQ 126
           FS+APM+D TD H R   RL+S++  LY+EM+    I++ K +   +LA+S ++HP+ LQ
Sbjct: 14  FSIAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGD---YLAYSEEEHPVALQ 73

Query: 127 IGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGEAMSVIA 186
           +GG++   +A+  +LA   GYDEINLN GCPS +V  +G FGA LM + + V + +  + 
Sbjct: 74  LGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQ-NGMFGACLMGNAQLVADCVKAMR 133

Query: 187 ANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENRNI 246
                PV+VK RIG+DD DSY  LCDFI  VS     + FIIH+RKA L+G+SP ENR I
Sbjct: 134 DVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREI 193

Query: 247 PPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPWRTLGHV 306
           PPL Y   Y L RDFP L  +INGGI +++E  A L+    GVMVGR AYQNP   L  V
Sbjct: 194 PPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQ-HMDGVMVGREAYQNP-GILAAV 253

Query: 307 DTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTY 339
           D  I+G+  +      V+     Y +  L  GTY
Sbjct: 254 DREIFGSSDTDADPVAVVRAMYPYIERELSQGTY 281

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy Swiss-Prot
Match: Q9CL29 (tRNA-dihydrouridine(20/20a) synthase OS=Pasteurella multocida (strain Pm70) OX=272843 GN=dusA PE=3 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 2.1e-59
Identity = 126/276 (45.65%), Postives = 171/276 (61.96%), Query Frame = 0


Query: 67  FSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQ 126
           FSVAPM+D T  H R   R  S+HA LY+EM+    I++ K +    L FSP ++P+ LQ
Sbjct: 13  FSVAPMLDWTTRHCRYFHRQFSQHALLYTEMVTTGAIIHAKYD---HLEFSPAENPVALQ 72

Query: 127 IGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGEAMSVIA 186
           +GG++   +A+  ++A   GY EINLN GCPS +V  +G FGA LM     V + +S + 
Sbjct: 73  LGGSDPTQLAQCAKIAQQRGYTEINLNVGCPSDRVQ-NGMFGACLMAKADLVADCVSAMQ 132

Query: 187 ANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENRNI 246
                PV+VK RIG+DD DSY  LC+F+ KV   +  + FIIH+RKA L+G+SP ENR I
Sbjct: 133 TEVSIPVTVKTRIGIDDLDSYEFLCEFVQKVHE-AGCQEFIIHARKAWLSGLSPKENREI 192

Query: 247 PPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPWRTLGHV 306
           PPL YE  Y L RDFP L  +INGGI T++E+   L+    GVMVGR AYQNP   LG++
Sbjct: 193 PPLDYERVYQLKRDFPHLLMSINGGIKTLEEMQQHLQY-MDGVMVGREAYQNP-SLLGYI 252

Query: 307 DTAIYGAPSSGITRRQVLEQYQVYGDSVL--GTYGN 341
           D A++      +T R+ +E+   Y +  L  G Y N
Sbjct: 253 DQALFDPTCPVVTPREAVEKMFPYIEQQLSQGVYLN 281

[Close]

BLAST of Cp4.1LG03g04570 vs. NCBI nr
Match: XP_023521322.1 (uncharacterized protein LOC111785077 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023526111.1 uncharacterized protein LOC111789688 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 855 bits (2208), Expect = 1.65e-311
Identity = 428/496 (86.29%), Postives = 428/496 (86.29%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 428

Query: 481 LRLMPPPYEDREQKVV 496
           LRLMPPPYEDREQKVV
Sbjct: 481 LRLMPPPYEDREQKVV 428

[Close]

BLAST of Cp4.1LG03g04570 vs. NCBI nr
Match: XP_023521321.1 (uncharacterized protein LOC111785077 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526107.1 uncharacterized protein LOC111789688 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526108.1 uncharacterized protein LOC111789688 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526109.1 uncharacterized protein LOC111789688 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852 bits (2202), Expect = 1.41e-310
Identity = 427/499 (85.57%), Postives = 429/499 (85.97%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 431

Query: 481 LRLMPPPYEDREQKVVLEA 499
           LRLMPPPYEDREQK + +A
Sbjct: 481 LRLMPPPYEDREQKSMDDA 431

[Close]

BLAST of Cp4.1LG03g04570 vs. NCBI nr
Match: XP_022934266.1 (uncharacterized protein LOC111441480 isoform X2 [Cucurbita moschata])

HSP 1 Score: 849 bits (2194), Expect = 2.24e-309
Identity = 425/496 (85.69%), Postives = 427/496 (86.09%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELA PYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAANT+APVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTNAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVM+GRAAYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 428

Query: 481 LRLMPPPYEDREQKVV 496
           LRLMPPPYEDREQKVV
Sbjct: 481 LRLMPPPYEDREQKVV 428

[Close]

BLAST of Cp4.1LG03g04570 vs. NCBI nr
Match: XP_022934264.1 (uncharacterized protein LOC111441480 isoform X1 [Cucurbita moschata] >XP_022934265.1 uncharacterized protein LOC111441480 isoform X1 [Cucurbita moschata])

HSP 1 Score: 847 bits (2188), Expect = 1.91e-308
Identity = 424/499 (84.97%), Postives = 428/499 (85.77%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELA PYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAANT+APVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTNAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVM+GRAAYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 431

Query: 481 LRLMPPPYEDREQKVVLEA 499
           LRLMPPPYEDREQK + +A
Sbjct: 481 LRLMPPPYEDREQKSMDDA 431

[Close]

BLAST of Cp4.1LG03g04570 vs. NCBI nr
Match: KAG7018078.1 (dusA, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 830 bits (2145), Expect = 1.29e-301
Identity = 419/495 (84.65%), Postives = 420/495 (84.85%), Query Frame = 0


Query: 11  ASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAGRYSPPWFSVA 70
           ASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAGRYSPPWFSVA
Sbjct: 24  ASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAGRYSPPWFSVA 83

Query: 71  PMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQIGGN 130
           PMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQIGGN
Sbjct: 84  PMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQIGGN 143

Query: 131 NLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPK------FVGEAMSV 190
           NLNNIAKAVELA PYGYDEINLNCGCPSPKVAGHGCFGARLMLDPK      FVGEAMSV
Sbjct: 144 NLNNIAKAVELAKPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKVSGCMLFVGEAMSV 203

Query: 191 IAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENR 250
           IAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENR
Sbjct: 204 IAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENR 263

Query: 251 NIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPWRTLG 310
           NIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVM+GRAAYQNPWRTLG
Sbjct: 264 NIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMMGRAAYQNPWRTLG 323

Query: 311 HVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTMAVIL 370
           HVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK               
Sbjct: 324 HVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK--------------- 383

Query: 371 HSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLLHLFY 430
                                                                PLLHLFY
Sbjct: 384 -----------------------------------------------------PLLHLFY 443

Query: 431 TDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANTLRLM 490
           TDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANTLRLM
Sbjct: 444 TDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANTLRLM 450

Query: 491 PPPYEDREQKVVLEA 499
           PPPYEDREQKVVLEA
Sbjct: 504 PPPYEDREQKVVLEA 450

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy TrEMBL
Match: A0A6J1F1C8 (uncharacterized protein LOC111441480 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441480 PE=4 SV=1)

HSP 1 Score: 849 bits (2194), Expect = 1.09e-309
Identity = 425/496 (85.69%), Postives = 427/496 (86.09%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELA PYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAANT+APVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTNAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVM+GRAAYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 428

Query: 481 LRLMPPPYEDREQKVV 496
           LRLMPPPYEDREQKVV
Sbjct: 481 LRLMPPPYEDREQKVV 428

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy TrEMBL
Match: A0A6J1F233 (uncharacterized protein LOC111441480 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441480 PE=4 SV=1)

HSP 1 Score: 847 bits (2188), Expect = 9.25e-309
Identity = 424/499 (84.97%), Postives = 428/499 (85.77%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG
Sbjct: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNLNNIAKAVELA PYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLNNIAKAVELAKPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAANT+APVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTNAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVM+GRAAYQNPW
Sbjct: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 431

Query: 481 LRLMPPPYEDREQKVVLEA 499
           LRLMPPPYEDREQK + +A
Sbjct: 481 LRLMPPPYEDREQKSMDDA 431

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy TrEMBL
Match: A0A1S3CGV7 (tRNA-dihydrouridine(20/20a) synthase-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500317 PE=4 SV=1)

HSP 1 Score: 815 bits (2106), Expect = 2.66e-296
Identity = 406/499 (81.36%), Postives = 417/499 (83.57%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSV+ASL+PV S IQKSHPRLSSSILQK L KP SW SGNQIL+Q PEAEMVAG
Sbjct: 1   MMKFAGCSVTASLTPVHSVIQKSHPRLSSSILQKKLSKPISWSSGNQILRQGPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMM+CTDNHYRTLARLISKHAWLY+EMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMECTDNHYRTLARLISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNL+NIAKA+ELANPYGYDEINLNCGCPSPKVAGHGCFG RLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLDNIAKAIELANPYGYDEINLNCGCPSPKVAGHGCFGVRLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIA+ TDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHF+IHSRKALLNGISP
Sbjct: 181 AMSVIASKTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFVIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENR IPPLKYEYFYALMRDFPDLRFTINGGINTVDE NAALRLGAHGVM+GRAAYQNPW
Sbjct: 241 AENRTIPPLKYEYFYALMRDFPDLRFTINGGINTVDEANAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLG YGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGRYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKAD AFQHCKTIKSFFEETLVAIPDY+LDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADAAFQHCKTIKSFFEETLVAIPDYVLDAPVAEPPSGREDLFANT 431

Query: 481 LRLMPPPYEDREQKVVLEA 499
           L LMPPPYEDREQKVVLEA
Sbjct: 481 LSLMPPPYEDREQKVVLEA 431

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy TrEMBL
Match: A0A0A0L9H3 (Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G690820 PE=4 SV=1)

HSP 1 Score: 814 bits (2102), Expect = 1.08e-295
Identity = 405/499 (81.16%), Postives = 416/499 (83.37%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCSV+AS +PVQS IQ SHPRLSSSILQKNL KP SW SGNQIL+Q PEAEMVAG
Sbjct: 1   MMKFAGCSVTASSTPVQSVIQNSHPRLSSSILQKNLSKPISWSSGNQILRQGPEAEMVAG 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMM+CTDNHYRTLARLISKHAWLY+EMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMECTDNHYRTLARLISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQIGGNNL+NIAKA+ELANPYGYDEINLNCGCPSPKVAGHGCFG RLMLDPKFVGE
Sbjct: 121 HPIVLQIGGNNLDNIAKAIELANPYGYDEINLNCGCPSPKVAGHGCFGVRLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAA TDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHF+IHSRKALLNGISP
Sbjct: 181 AMSVIAAKTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFVIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENR IPPLKYEYFYALMRDFPDLRFTINGGINTVDE NAALRLGAHGVM+GRAAYQNPW
Sbjct: 241 AENRTIPPLKYEYFYALMRDFPDLRFTINGGINTVDEANAALRLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLG YGNKPNIRDVVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGRYGNKPNIRDVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFYTDPGNGPWKRKAD AF HCKTIKSFFEET+VAIPDY+LDAPVAEPPSGREDLFANT
Sbjct: 421 HLFYTDPGNGPWKRKADAAFMHCKTIKSFFEETIVAIPDYVLDAPVAEPPSGREDLFANT 431

Query: 481 LRLMPPPYEDREQKVVLEA 499
           L LMPPPYEDREQKVVLEA
Sbjct: 481 LSLMPPPYEDREQKVVLEA 431

[Close]

BLAST of Cp4.1LG03g04570 vs. ExPASy TrEMBL
Match: A0A6J1DED7 (uncharacterized protein LOC111020285 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111020285 PE=4 SV=1)

HSP 1 Score: 790 bits (2040), Expect = 3.01e-286
Identity = 393/499 (78.76%), Postives = 412/499 (82.57%), Query Frame = 0


Query: 1   MMKFAGCSVSASLSPVQSFIQKSHPRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAG 60
           MMKFAGCS++ASL+P+QS IQKSH RLSS+ILQ+NL KPS W S NQ L+Q PEAEMVA 
Sbjct: 1   MMKFAGCSITASLTPIQSVIQKSHCRLSSNILQENLSKPSGWSSSNQKLRQGPEAEMVAR 60

Query: 61  RYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQ 120
           RYSPPWFSVAPMMD TDNHYRTLARLISKHAWLY+EMLAAETIVYQKDNLDRFLAFSPDQ
Sbjct: 61  RYSPPWFSVAPMMDWTDNHYRTLARLISKHAWLYTEMLAAETIVYQKDNLDRFLAFSPDQ 120

Query: 121 HPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGE 180
           HPIVLQ+GGNNLNNIAKA ELAN YGYDEIN NCGCPS KVAGHGCFG RLMLDPKFVGE
Sbjct: 121 HPIVLQLGGNNLNNIAKATELANAYGYDEINFNCGCPSAKVAGHGCFGVRLMLDPKFVGE 180

Query: 181 AMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISP 240
           AMSVIAANTD PVSVKCRIGVDDHDSYNELCDFIYKVSSLSPT+HFIIHSRKALLNGISP
Sbjct: 181 AMSVIAANTDVPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTRHFIIHSRKALLNGISP 240

Query: 241 AENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPW 300
           AENR+IPPLKYEYFYALMRDFPDLRFTINGGIN+VDEVNAAL+LGAHGVM+GRAAYQNPW
Sbjct: 241 AENRSIPPLKYEYFYALMRDFPDLRFTINGGINSVDEVNAALKLGAHGVMMGRAAYQNPW 300

Query: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTM 360
           RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIR+VVK           
Sbjct: 301 RTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIREVVK----------- 360

Query: 361 AVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLL 420
                                                                    PLL
Sbjct: 361 ---------------------------------------------------------PLL 420

Query: 421 HLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLFANT 480
           HLFY+DPGNGPWKRKAD AFQHCKTIKSFFEETLVA+PDYILDAPVAEPP GREDLFANT
Sbjct: 421 HLFYSDPGNGPWKRKADAAFQHCKTIKSFFEETLVAVPDYILDAPVAEPPPGREDLFANT 431

Query: 481 LRLMPPPYEDREQKVVLEA 499
           L L+PPPYED+EQKVVLEA
Sbjct: 481 LSLLPPPYEDKEQKVVLEA 431

[Close]

BLAST of Cp4.1LG03g04570 vs. TAIR 10
Match: AT5G47970.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 570.9 bits (1470), Expect = 1.0e-162
Identity = 274/439 (62.41%), Postives = 321/439 (73.12%), Query Frame = 0


Query: 58  VAGRYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFS 117
           V+  YSPP FS+APMM  TDNHYRTLARLI+KHAWLY+EMLAAETIVYQ+DNLD FLAFS
Sbjct: 3   VSEAYSPPLFSIAPMMGWTDNHYRTLARLITKHAWLYTEMLAAETIVYQEDNLDSFLAFS 62

Query: 118 PDQHPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKF 177
           PDQHPIVLQIGG NL N+AKA  LAN Y YDEIN NCGCPSPKV+G GCFGA LMLDPKF
Sbjct: 63  PDQHPIVLQIGGRNLENLAKATRLANAYAYDEINFNCGCPSPKVSGRGCFGALLMLDPKF 122

Query: 178 VGEAMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNG 237
           VGEAMSVIAANT+A V+VKCRIGVDDHDSYNELCDFI+ VSSLSPTKHFIIHSRKALL+G
Sbjct: 123 VGEAMSVIAANTNAAVTVKCRIGVDDHDSYNELCDFIHIVSSLSPTKHFIIHSRKALLSG 182

Query: 238 ISPAENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQ 297
           +SP++NR IPPLKYE+F+AL+RDFPDL+FTINGGIN+V E +AALR GAHGVM+GRA Y 
Sbjct: 183 LSPSDNRRIPPLKYEFFFALLRDFPDLKFTINGGINSVVEADAALRSGAHGVMLGRAVYY 242

Query: 298 NPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLI 357
           NPW  LGHVDT +YG+PSSGITRRQVLE+Y++YG+SVLG YG                  
Sbjct: 243 NPWHILGHVDTVVYGSPSSGITRRQVLEKYKLYGESVLGKYGK----------------- 302

Query: 358 LTMAVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK 417
                                                              +PN+RD+V+
Sbjct: 303 --------------------------------------------------GRPNLRDIVR 362

Query: 418 PLLHLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLF 477
           PL++LF+++ GNG WKR+ D A  HC T++SF +E L AIPDY+LD+   +  +GREDLF
Sbjct: 363 PLINLFHSESGNGQWKRRTDAALLHCTTLQSFLDEVLPAIPDYVLDSSAVKEATGREDLF 374

Query: 478 ANTLRLMPPPYEDREQKVV 497
           A+  RL+PPPYE    K +
Sbjct: 423 ADVQRLLPPPYEKESFKAL 374

[Close]

BLAST of Cp4.1LG03g04570 vs. TAIR 10
Match: AT5G47970.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 570.9 bits (1470), Expect = 1.0e-162
Identity = 274/439 (62.41%), Postives = 321/439 (73.12%), Query Frame = 0


Query: 58  VAGRYSPPWFSVAPMMDCTDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFS 117
           V+  YSPP FS+APMM  TDNHYRTLARLI+KHAWLY+EMLAAETIVYQ+DNLD FLAFS
Sbjct: 3   VSEAYSPPLFSIAPMMGWTDNHYRTLARLITKHAWLYTEMLAAETIVYQEDNLDSFLAFS 62

Query: 118 PDQHPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKF 177
           PDQHPIVLQIGG NL N+AKA  LAN Y YDEIN NCGCPSPKV+G GCFGA LMLDPKF
Sbjct: 63  PDQHPIVLQIGGRNLENLAKATRLANAYAYDEINFNCGCPSPKVSGRGCFGALLMLDPKF 122

Query: 178 VGEAMSVIAANTDAPVSVKCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNG 237
           VGEAMSVIAANT+A V+VKCRIGVDDHDSYNELCDFI+ VSSLSPTKHFIIHSRKALL+G
Sbjct: 123 VGEAMSVIAANTNAAVTVKCRIGVDDHDSYNELCDFIHIVSSLSPTKHFIIHSRKALLSG 182

Query: 238 ISPAENRNIPPLKYEYFYALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQ 297
           +SP++NR IPPLKYE+F+AL+RDFPDL+FTINGGIN+V E +AALR GAHGVM+GRA Y 
Sbjct: 183 LSPSDNRRIPPLKYEFFFALLRDFPDLKFTINGGINSVVEADAALRSGAHGVMLGRAVYY 242

Query: 298 NPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLI 357
           NPW  LGHVDT +YG+PSSGITRRQVLE+Y++YG+SVLG YG                  
Sbjct: 243 NPWHILGHVDTVVYGSPSSGITRRQVLEKYKLYGESVLGKYGK----------------- 302

Query: 358 LTMAVILHSPWRTLGHVDTAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVK 417
                                                              +PN+RD+V+
Sbjct: 303 --------------------------------------------------GRPNLRDIVR 362

Query: 418 PLLHLFYTDPGNGPWKRKADFAFQHCKTIKSFFEETLVAIPDYILDAPVAEPPSGREDLF 477
           PL++LF+++ GNG WKR+ D A  HC T++SF +E L AIPDY+LD+   +  +GREDLF
Sbjct: 363 PLINLFHSESGNGQWKRRTDAALLHCTTLQSFLDEVLPAIPDYVLDSSAVKEATGREDLF 374

Query: 478 ANTLRLMPPPYEDREQKVV 497
           A+  RL+PPPYE    K +
Sbjct: 423 ADVQRLLPPPYEKESFKAL 374

[Close]

BLAST of Cp4.1LG03g04570 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 3.9e-162
Identity = 280/473 (59.20%), Postives = 339/473 (71.67%), Query Frame = 0


Query: 25  PRLSSSILQKNLYKPSSWFSGNQILQQSPEAEMVAGRYSPPWFSVAPMMDCTDNHYRTLA 84
           P +S    +  + KP++ FS       SP        Y PP FSVAPMMD TDNHYRTLA
Sbjct: 24  PNVSEMFFKSPMLKPTARFSST----SSP--------YLPPSFSVAPMMDWTDNHYRTLA 83

Query: 85  RLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQIGGNNLNNIAKAVELANP 144
           RLI+KHAWLY+EM+AAET+V+Q+ NLDRFLAFSP QHPIVLQ+GG+N+ N+AKA +L++ 
Sbjct: 84  RLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIVLQLGGSNVENLAKAAKLSDA 143

Query: 145 YGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGEAMSVIAANTDAPVSVKCRIGVDDH 204
           YGYDEINLNCGCPSPKVAGHGCFG  LML PK VGEAMS IAANT+ PV+VKCRIGVD+H
Sbjct: 144 YGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSAIAANTNVPVTVKCRIGVDNH 203

Query: 205 DSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENRNIPPLKYEYFYALMRDFPDL 264
           DSY+ELCDFIYKVS+LSPT+HFI+HSRKALL GISPA+NR IPPLKYEY+YAL+RDFPDL
Sbjct: 204 DSYDELCDFIYKVSTLSPTRHFIVHSRKALLGGISPADNRRIPPLKYEYYYALVRDFPDL 263

Query: 265 RFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPWRTLGHVDTAIYGAPSSGITRRQVL 324
           RFTINGGI +V +VNAAL+ GAHGVMVGRAAY NPW+TLG VDTA+YG PSSG+TRRQVL
Sbjct: 264 RFTINGGITSVSKVNAALKEGAHGVMVGRAAYNNPWQTLGQVDTAVYGVPSSGLTRRQVL 323

Query: 325 EQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTMAVILHSPWRTLGHVDTAIYGAPSS 384
           EQYQVYGDSVLGT+GN                                            
Sbjct: 324 EQYQVYGDSVLGTHGN-------------------------------------------- 383

Query: 385 GITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLLHLFYTDPGNGPWKRKADFAFQHCK 444
                                   +PN+RD+VKPLL+LF+++ GN  WKR+AD AF+ C+
Sbjct: 384 -----------------------GRPNVRDLVKPLLNLFHSENGNSLWKRRADAAFKECR 417

Query: 445 TIKSFFEETLVAIPDYILDAPVA-EPPSGREDLFANTLRLMPPPYEDREQKVV 497
           ++ S  EE+L AIPD +LD+P++  P SG ED+FA+   ++PPPYE  E+ ++
Sbjct: 444 SVGSLLEESLRAIPDCVLDSPISGSPESGDEDVFADVHNVLPPPYEAGEEIIL 417

[Close]

BLAST of Cp4.1LG03g04570 vs. TAIR 10
Match: AT3G63510.2 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 543.9 bits (1400), Expect = 1.4e-154
Identity = 260/422 (61.61%), Postives = 315/422 (74.64%), Query Frame = 0


Query: 76  TDNHYRTLARLISKHAWLYSEMLAAETIVYQKDNLDRFLAFSPDQHPIVLQIGGNNLNNI 135
           +DNHYRTLARLI+KHAWLY+EM+AAET+V+Q+ NLDRFLAFSP QHPIVLQ+GG+N+ N+
Sbjct: 30  SDNHYRTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIVLQLGGSNVENL 89

Query: 136 AKAVELANPYGYDEINLNCGCPSPKVAGHGCFGARLMLDPKFVGEAMSVIAANTDAPVSV 195
           AKA +L++ YGYDEINLNCGCPSPKVAGHGCFG  LML PK VGEAMS IAANT+ PV+V
Sbjct: 90  AKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSAIAANTNVPVTV 149

Query: 196 KCRIGVDDHDSYNELCDFIYKVSSLSPTKHFIIHSRKALLNGISPAENRNIPPLKYEYFY 255
           KCRIGVD+HDSY+ELCDFIYKVS+LSPT+HFI+HSRKALL GISPA+NR IPPLKYEY+Y
Sbjct: 150 KCRIGVDNHDSYDELCDFIYKVSTLSPTRHFIVHSRKALLGGISPADNRRIPPLKYEYYY 209

Query: 256 ALMRDFPDLRFTINGGINTVDEVNAALRLGAHGVMVGRAAYQNPWRTLGHVDTAIYGAPS 315
           AL+RDFPDLRFTINGGI +V +VNAAL+ GAHGVMVGRAAY NPW+TLG VDTA+YG PS
Sbjct: 210 ALVRDFPDLRFTINGGITSVSKVNAALKEGAHGVMVGRAAYNNPWQTLGQVDTAVYGVPS 269

Query: 316 SGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKSWLCLSLILTMAVILHSPWRTLGHVD 375
           SG+TRRQVLEQYQVYGDSVLGT+GN                                   
Sbjct: 270 SGLTRRQVLEQYQVYGDSVLGTHGN----------------------------------- 329

Query: 376 TAIYGAPSSGITRRQVLEQYQVYGDSVLGTYGNKPNIRDVVKPLLHLFYTDPGNGPWKRK 435
                                            +PN+RD+VKPLL+LF+++ GN  WKR+
Sbjct: 330 --------------------------------GRPNVRDLVKPLLNLFHSENGNSLWKRR 384

Query: 436 ADFAFQHCKTIKSFFEETLVAIPDYILDAPVA-EPPSGREDLFANTLRLMPPPYEDREQK 495
           AD AF+ C+++ S  EE+L AIPD +LD+P++  P SG ED+FA+   ++PPPYE  E+ 
Sbjct: 390 ADAAFKECRSVGSLLEESLRAIPDCVLDSPISGSPESGDEDVFADVHNVLPPPYEAGEEI 384

Query: 496 VV 497
           ++
Sbjct: 450 IL 384

[Close]

BLAST of Cp4.1LG03g04570 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.6e-09
Identity = 43/164 (26.22%), Postives = 77/164 (46.95%), Query Frame = 0


Query: 39  PSSWFSGNQILQQSPEAEMVAGRYSP---PWFSVAPMMDCTDNHYRTLARLISKHAWLYS 98
           P+S  S +++L      E     +     P + VAPM+D ++  +R L +     A  Y+
Sbjct: 59  PASLGSPSRVLSIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQA-AYT 118

Query: 99  EMLAAETIVYQKDNLDRFLAFSPDQHPIVLQIGGNNLNNIAKAVELANPYGYDEINLNCG 158
            ML +      +   ++      +  P+ +Q   N+ + + +A +   PY  D +++N G
Sbjct: 119 PMLHSRIFTETEKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPY-CDYVDINLG 178

Query: 159 CPSPKVAGHGCFGARLMLDPKFVGEAMSVIAANTDAPVSVKCRI 200
           CP  ++A  G +GA LM +   V   +  +A N + PVS K RI
Sbjct: 179 CPQ-RIARRGNYGAFLMDNLPLVKSLVEKLAQNLNVPVSCKIRI 219

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g04570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8X5V6	3.2e-60	43.81	tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O157:H7 OX=83334 GN=dus...	[more]
P32695	3.2e-60	43.81	tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli (strain K12) OX=83333 G...	[more]
Q7UBC5	1.6e-59	46.35	tRNA-dihydrouridine(20/20a) synthase OS=Shigella flexneri OX=623 GN=dusA PE=3 SV...	[more]
Q8FB30	2.1e-59	45.99	tRNA-dihydrouridine(20/20a) synthase OS=Escherichia coli O6:H1 (strain CFT073 / ...	[more]
Q9CL29	2.1e-59	45.65	tRNA-dihydrouridine(20/20a) synthase OS=Pasteurella multocida (strain Pm70) OX=2...	[more]

BLAST of Cp4.1LG03g04570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023521322.1	1.65e-311	86.29	uncharacterized protein LOC111785077 isoform X2 [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_023521321.1	1.41e-310	85.57	uncharacterized protein LOC111785077 isoform X1 [Cucurbita pepo subsp. pepo] >XP...	[more]
XP_022934266.1	2.24e-309	85.69	uncharacterized protein LOC111441480 isoform X2 [Cucurbita moschata]	[more]
XP_022934264.1	1.91e-308	84.97	uncharacterized protein LOC111441480 isoform X1 [Cucurbita moschata] >XP_0229342...	[more]
KAG7018078.1	1.29e-301	84.65	dusA, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Cp4.1LG03g04570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1C8	1.09e-309	85.69	uncharacterized protein LOC111441480 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1F233	9.25e-309	84.97	uncharacterized protein LOC111441480 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A1S3CGV7	2.66e-296	81.36	tRNA-dihydrouridine(20/20a) synthase-like isoform X1 OS=Cucumis melo OX=3656 GN=...	[more]
A0A0A0L9H3	1.08e-295	81.16	Dus domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G690820 PE=4 SV...	[more]
A0A6J1DED7	3.01e-286	78.76	uncharacterized protein LOC111020285 isoform X1 OS=Momordica charantia OX=3673 G...	[more]

BLAST of Cp4.1LG03g04570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47970.1	1.0e-162	62.41	Aldolase-type TIM barrel family protein	[more]
AT5G47970.2	1.0e-162	62.41	Aldolase-type TIM barrel family protein	[more]
AT3G63510.1	3.9e-162	59.20	FMN-linked oxidoreductases superfamily protein	[more]
AT3G63510.2	1.4e-154	61.61	FMN-linked oxidoreductases superfamily protein	[more]
AT5G67220.1	1.6e-09	26.22	FMN-linked oxidoreductases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR035587	DUS-like, FMN-binding domain	PFAM	PF01207	Dus	coord: 69..350 e-value: 2.0E-56 score: 191.3
IPR035587	DUS-like, FMN-binding domain	CDD	cd02801	DUS_like_FMN	coord: 67..300 e-value: 2.19008E-79 score: 245.482
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 63..304 e-value: 6.2E-70 score: 237.4
None	No IPR available	PANTHER	PTHR42907:SF2	TRNA-DIHYDROURIDINE SYNTHASE	coord: 57..353
None	No IPR available	PANTHER	PTHR42907:SF2	TRNA-DIHYDROURIDINE SYNTHASE	coord: 363..496
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 66..354
IPR004653	tRNA-dihydrouridine(20/20a) synthase	PANTHER	PTHR42907	FMN-LINKED OXIDOREDUCTASES SUPERFAMILY PROTEIN	coord: 57..353 coord: 363..496
IPR018517	tRNA-dihydrouridine synthase, conserved site	PROSITE	PS01136	UPF0034	coord: 150..169

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g04570.1	Cp4.1LG03g04570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002943	tRNA dihydrouridine synthesis
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0017150	tRNA dihydrouridine synthase activity
molecular_function	GO:0003824	catalytic activity