Cp4.1LG03g04160 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG03g04160
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCytochrome P450, putative
LocationCp4.1LG03: 2302259 .. 2304231 (+)
RNA-Seq ExpressionCp4.1LG03g04160
SyntenyCp4.1LG03g04160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCTGTGTTGGGACTATTTTGTTCATCACTTTTTTCTCTGTTTTCTTTTACCTTCGAAAATCACGAAGCCATGAAGGTTTTAGACTCTACCCTGTTGTCGGAGTTCTGCCGCTGTTCTTATTGAACCGGCACCGTTTTCTGGACTGGACGACGGAGGTCCTCCGCAACAGTCGGAACAACACGGCGGTATTCCGCCGGCCAGGGGGTATCACCGGCGTCATGACGGGAAACCCAGATGTGGTGGAGCATATACTGAAGACCCAGTTTGAGAACTACTCGAAAGGGGAACGATTGGTTTCGCTTATGGAGGATTTTTTGGGGTCTGGAATTTTCAACTCCGACGGCGAAATTTGGAGGATTCAACGGAAAATGGCGAGTTATGAATTCAGCACCAAATCGCTGAGAAATTTCGTCACGGAAAATGTTGTAATTGAGATTCAAACGAAGCTGATTCCTCTGTTGGAAAAGGCTTCTGAAACAGAGCAGATTTTGGATTTTCAAGACGTTTTTGAACGATTTGCGTTTGATAATGTCTGTAAATTATCGTTCAATTTCGACCCTGGTTGTCTCGGCGGCGATGCAACTTCCGCCACCGAGTTCATGCTCGCTTTTGAGACCGCCGCCACTCTAATTTCCGGCAGGTTCTGTTTTTTTTTTTTTTTTTTCTCTCCGATCTGCATTTTTAATAAAAAAAAAACCATACCCATTTCGTGCTACTAAACACGAAGATATAATCCACTTTGAGTATAAGCTCTCTGACTTTGTTTTGGCTTATGTACGACGCCTTATACCGGTAGATGTGTATTCCTTGTTTATAAACTTATGATCAGTGTCTGATAAAAAGTGTACTTTGTTCAAGGGCTCTAGCGAAGGAGTCGAGCCGGGAGGCTGTTTGAGGGCTTCATAGGCGTTTGGGGAGGCTAGAGTGTAGGAAGATTGTTGGGAGGATTGTTGGGAGGGAGTCCTACATTAGCTACTTTAGGAATGATCGTGGGTTTACAAGTAAAGAATATATCTACATTGTTATGAGTCTGTTTGGGGAAACCGAAGGCAGAGTCATTAGAGCTTATTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTAACATTTTGTTCTTAATTTGAACAGATTTAGGTATGCAATTCCTAAATGGTACAAGATCAAGAAATTTTTCAATTTGGGATCAGAGAGAACCCTGAAGAAATCCATATCGATAGTTCATAAATTTGTAGACAACATCATACGCTCAAGAATGGAAGAGAAAACGACCGAAAACAAGGATGACAAAGATTTATTATCTCGGTTCATGGCCGACAAACAAAATTCCCCAAAATTCCTCCGAGACATAGCCATAAGCTTCATCCTCGCCGGTCGAGACACCACTTCTTCAGCTCTAACATGGTTCTTTTGGATCATATCCACCCGACCAGACATCAAACAAAAGATCCTTAAAGAACTCAAAACCATCAGGTCTAAAACTAGTAACCAAATCGGAGAAACCTTCCACATCGACGAACTCCGGGACATGCATTATCTCCAAGCAACATTGTCGGAGACACTCCGGTTATACCCACCGGTGCCGGTGGACCCAAAAGCTTGTCGAGGCGACGATGTCCTACCGGACGGGACGGTTGTCGGGAAGAATTGGTTTGTGATGTACCATGCATATGCAATGGGGAGAATGGAGAGCATTTGGGGGAAGAATTGTGAGGAGTTTTGGCCGGAAAGGTGGCTGGAAAATGGGGTTTGTAGGGGTGAGAGCCCTTTTAGGTTCCCTATATTCCATGCAGGGCCGAGGACGTGCGTGGGGAAAGACATGGCTTATGTTCAGATGAAGGCCATAGTTGCAGCTGTGATGGAGAGGTTTGAGGTCATAATCATGAAGAAGGAGACTCCAAAGCATTATCAGTCATTGACTTTGAGGATGGAGAACGGGTTGCAAGTTATGGTCGAAAAGAGGTCAATATAA

mRNA sequence

GTCTGTGTTGGGACTATTTTGTTCATCACTTTTTTCTCTGTTTTCTTTTACCTTCGAAAATCACGAAGCCATGAAGGTTTTAGACTCTACCCTGTTGTCGGAGTTCTGCCGCTGTTCTTATTGAACCGGCACCGTTTTCTGGACTGGACGACGGAGGTCCTCCGCAACAGTCGGAACAACACGGCGGTATTCCGCCGGCCAGGGGGTATCACCGGCGTCATGACGGGAAACCCAGATGTGGTGGAGCATATACTGAAGACCCAGTTTGAGAACTACTCGAAAGGGGAACGATTGGTTTCGCTTATGGAGGATTTTTTGGGGTCTGGAATTTTCAACTCCGACGGCGAAATTTGGAGGATTCAACGGAAAATGGCGAGTTATGAATTCAGCACCAAATCGCTGAGAAATTTCGTCACGGAAAATGTTGTAATTGAGATTCAAACGAAGCTGATTCCTCTGTTGGAAAAGGCTTCTGAAACAGAGCAGATTTTGGATTTTCAAGACGTTTTTGAACGATTTGCGTTTGATAATGTCTGTAAATTATCGTTCAATTTCGACCCTGGTTGTCTCGGCGGCGATGCAACTTCCGCCACCGAGTTCATGCTCGCTTTTGAGACCGCCGCCACTCTAATTTCCGGCAGAACCCTGAAGAAATCCATATCGATAGTTCATAAATTTGTAGACAACATCATACGCTCAAGAATGGAAGAGAAAACGACCGAAAACAAGGATGACAAAGATTTATTATCTCGGTTCATGGCCGACAAACAAAATTCCCCAAAATTCCTCCGAGACATAGCCATAAGCTTCATCCTCGCCGGTCGAGACACCACTTCTTCAGCTCTAACATGGTTCTTTTGGATCATATCCACCCGACCAGACATCAAACAAAAGATCCTTAAAGAACTCAAAACCATCAGGTCTAAAACTAGTAACCAAATCGGAGAAACCTTCCACATCGACGAACTCCGGGACATGCATTATCTCCAAGCAACATTGTCGGAGACACTCCGGTTATACCCACCGGTGCCGGTGGACCCAAAAGCTTGTCGAGGCGACGATGTCCTACCGGACGGGACGGTTGTCGGGAAGAATTGGTTTGTGATGTACCATGCATATGCAATGGGGAGAATGGAGAGCATTTGGGGGAAGAATTGTGAGGAGTTTTGGCCGGAAAGGTGGCTGGAAAATGGGGTTTGTAGGGGTGAGAGCCCTTTTAGGTTCCCTATATTCCATGCAGGGCCGAGGACGTGCGTGGGGAAAGACATGGCTTATGTTCAGATGAAGGCCATAGTTGCAGCTGTGATGGAGAGGTTTGAGGTCATAATCATGAAGAAGGAGACTCCAAAGCATTATCAGTCATTGACTTTGAGGATGGAGAACGGGTTGCAAGTTATGGTCGAAAAGAGGTCAATATAA

Coding sequence (CDS)

GTCTGTGTTGGGACTATTTTGTTCATCACTTTTTTCTCTGTTTTCTTTTACCTTCGAAAATCACGAAGCCATGAAGGTTTTAGACTCTACCCTGTTGTCGGAGTTCTGCCGCTGTTCTTATTGAACCGGCACCGTTTTCTGGACTGGACGACGGAGGTCCTCCGCAACAGTCGGAACAACACGGCGGTATTCCGCCGGCCAGGGGGTATCACCGGCGTCATGACGGGAAACCCAGATGTGGTGGAGCATATACTGAAGACCCAGTTTGAGAACTACTCGAAAGGGGAACGATTGGTTTCGCTTATGGAGGATTTTTTGGGGTCTGGAATTTTCAACTCCGACGGCGAAATTTGGAGGATTCAACGGAAAATGGCGAGTTATGAATTCAGCACCAAATCGCTGAGAAATTTCGTCACGGAAAATGTTGTAATTGAGATTCAAACGAAGCTGATTCCTCTGTTGGAAAAGGCTTCTGAAACAGAGCAGATTTTGGATTTTCAAGACGTTTTTGAACGATTTGCGTTTGATAATGTCTGTAAATTATCGTTCAATTTCGACCCTGGTTGTCTCGGCGGCGATGCAACTTCCGCCACCGAGTTCATGCTCGCTTTTGAGACCGCCGCCACTCTAATTTCCGGCAGAACCCTGAAGAAATCCATATCGATAGTTCATAAATTTGTAGACAACATCATACGCTCAAGAATGGAAGAGAAAACGACCGAAAACAAGGATGACAAAGATTTATTATCTCGGTTCATGGCCGACAAACAAAATTCCCCAAAATTCCTCCGAGACATAGCCATAAGCTTCATCCTCGCCGGTCGAGACACCACTTCTTCAGCTCTAACATGGTTCTTTTGGATCATATCCACCCGACCAGACATCAAACAAAAGATCCTTAAAGAACTCAAAACCATCAGGTCTAAAACTAGTAACCAAATCGGAGAAACCTTCCACATCGACGAACTCCGGGACATGCATTATCTCCAAGCAACATTGTCGGAGACACTCCGGTTATACCCACCGGTGCCGGTGGACCCAAAAGCTTGTCGAGGCGACGATGTCCTACCGGACGGGACGGTTGTCGGGAAGAATTGGTTTGTGATGTACCATGCATATGCAATGGGGAGAATGGAGAGCATTTGGGGGAAGAATTGTGAGGAGTTTTGGCCGGAAAGGTGGCTGGAAAATGGGGTTTGTAGGGGTGAGAGCCCTTTTAGGTTCCCTATATTCCATGCAGGGCCGAGGACGTGCGTGGGGAAAGACATGGCTTATGTTCAGATGAAGGCCATAGTTGCAGCTGTGATGGAGAGGTTTGAGGTCATAATCATGAAGAAGGAGACTCCAAAGCATTATCAGTCATTGACTTTGAGGATGGAGAACGGGTTGCAAGTTATGGTCGAAAAGAGGTCAATATAA

Protein sequence

VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRIQRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCKLSFNFDPGCLGGDATSATEFMLAFETAATLISGRTLKKSISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRMENGLQVMVEKRSI
Homology
BLAST of Cp4.1LG03g04160 vs. ExPASy Swiss-Prot
Match: O81117 (Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 2.3e-100
Identity = 197/471 (41.83%), Postives = 289/471 (61.36%), Query Frame = 0

Query: 30  YPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAVFRRPGGITGVMTGNPDVVEHILKTQF 89
           YP++G    F  N HR + W +++++ S + T       G   ++TGNP  V+HILK QF
Sbjct: 51  YPLIGSYLSFRKNLHRRIQWLSDIVQISPSATFQLDGTLGKRQIITGNPSTVQHILKNQF 110

Query: 90  ENYSKGERLVSLMEDFLGSGIFNSDGEIWRIQRKMASYEFSTKSLRNFVTENVVIEIQTK 149
            NY KG    + + DFLG+GIFN++G  W+ QR++AS+EF+TKS+RNFV   V  E+  +
Sbjct: 111 SNYQKGTTFTNTLSDFLGTGIFNTNGPNWKFQRQVASHEFNTKSIRNFVEHIVDTELTNR 170

Query: 150 LIPLLEKASETEQILDFQDVFERFAFDNVCKLSFNFDPGCLGGDATSATEFMLAFETAAT 209
           LIP+L  +++T  ILDFQD+ +RF FDN+C ++F +DP  L   +T+ ++F  A+E A  
Sbjct: 171 LIPILTSSTQTNNILDFQDILQRFTFDNICNIAFGYDPEYL-TPSTNRSKFAEAYEDATE 230

Query: 210 LISGR---------------------TLKKSISIVHKFVDNIIRSRMEE-KTTENKDDKD 269
           + S R                      LK++++ V  F   ++R +  E +   + + +D
Sbjct: 231 ISSKRFRLPLPIIWKIKKYFNIGSEKRLKEAVTEVRSFAKKLVREKKRELEEKSSLETED 290

Query: 270 LLSRFMADKQNSPKFLRDIAISFILAGRDTTSSALTWFFWIISTRPDIKQKILKELKTIR 329
           +LSRF++   +   F+ DI ISFILAG+DTTS+ALTWFFW++   P ++++I+ EL    
Sbjct: 291 MLSRFLSSGHSDEDFVADIVISFILAGKDTTSAALTWFFWLLWKNPRVEEEIVNEL---- 350

Query: 330 SKTSNQIGETFHIDELRDMHYLQATLSETLRLYPPVPVDPKACRGDDVLPDGTVVGKNWF 389
           SK S    E    DE+++M Y  A LSE++RLYPPVP+D K    DDVLPDG VV K   
Sbjct: 351 SKKS----ELMVYDEVKEMVYTHAALSESMRLYPPVPMDSKEAVNDDVLPDGWVVKKGTI 410

Query: 390 VMYHAYAMGRMESIWGKNCEEFWPERWLE----NG--VCRGESPFRFPIFHAGPRTCVGK 449
           V YH YAMGRM+S+WG +  EF PERWLE    NG  V  G   + +P+F AGPR C+GK
Sbjct: 411 VTYHVYAMGRMKSLWGDDWAEFRPERWLEKDEVNGKWVFVGRDSYSYPVFQAGPRVCLGK 470

Query: 450 DMAYVQMKAIVAAVMERFEVI--IMKKETPKHYQSLTLRMENGLQVMVEKR 471
           +MA++QMK IVA ++ +F+V+      + P     L+ +ME G  V ++KR
Sbjct: 471 EMAFMQMKRIVAGIVGKFKVVPEAHLAQEPGFISFLSSQMEGGFPVTIQKR 512

BLAST of Cp4.1LG03g04160 vs. ExPASy Swiss-Prot
Match: P98188 (Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.4e-97
Identity = 195/474 (41.14%), Postives = 282/474 (59.49%), Query Frame = 0

Query: 30  YPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAVFRRPGGITGVMTGNPDVVEHILKTQF 89
           YP+ G     L N HR + WT+++L+   ++T V  RP G   V T  P VV+HIL+T F
Sbjct: 46  YPIFGSAFSLLANFHRRIQWTSDILQTIPSSTFVLHRPFGARQVFTAQPAVVQHILRTNF 105

Query: 90  ENYSKGERLVSLMEDFLGSGIFNSDGEIWRIQRKMASYEFSTKSLRNFVTENVVIEIQTK 149
             Y KG      + DFLG GIFN+DGE W+ QR+++S+EF+T+SLR FV   V +E+  +
Sbjct: 106 TCYGKGLTFYQSINDFLGDGIFNADGESWKFQRQISSHEFNTRSLRKFVETVVDVELSDR 165

Query: 150 LIPLLEKASETEQILDFQDVFERFAFDNVCKLSFNFDPGCLGGDATSATEFMLAFETAAT 209
           L+P+L +AS ++  LDFQD+ +R  FDN+C ++F +DP  L   +     F  AF+ ++ 
Sbjct: 166 LVPVLSQASNSQTTLDFQDILQRLTFDNICMIAFGYDPEYL-LPSLPEIPFAKAFDESSQ 225

Query: 210 LI---------------------SGRTLKKSISIVHKFVDNIIRSR---MEEKTTENKDD 269
           L                        R LK++++ V      I++++   ++EK  +++ +
Sbjct: 226 LSIERLNALIPLLWKVKRFLNIGVERQLKEAVAEVRGLATKIVKNKKKELKEKALQSESE 285

Query: 270 K-DLLSRFMADKQNSPKFLRDIAISFILAGRDTTSSALTWFFWIISTRPDIKQKILKELK 329
             DLLSRF++   +   F+ D+ IS ILAGRDTTS+ALTWFFW++S    ++ +ILKE  
Sbjct: 286 SVDLLSRFLSSGHSDESFVTDMVISIILAGRDTTSAALTWFFWLLSKHSHVENEILKE-- 345

Query: 330 TIRSKTSNQIGETFHIDELRDMHYLQATLSETLRLYPPVPVDPKACRGDDVLPDGTVVGK 389
            I  K+     ET   DE++DM Y  A L E++RLYPP+PVD K    DDVLPDGT+V K
Sbjct: 346 -ITGKS-----ETVGYDEVKDMVYTHAALCESMRLYPPLPVDTKVAVHDDVLPDGTLVKK 405

Query: 390 NWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGVCR-----GESPFRFPIFHAGPRTCV 449
            W V YH YAMGR E IWG +  EF PERWL           G   + +P+F AGPR C+
Sbjct: 406 GWRVTYHIYAMGRSEKIWGPDWAEFRPERWLSRDEVGKWSFVGIDYYSYPVFQAGPRVCI 465

Query: 450 GKDMAYVQMKAIVAAVMERFEVI--IMKKETPKHYQSLTLRMENGLQVMVEKRS 472
           GK+MA++QMK +VA +M RF V+  +++   P++    T  M+ G  V +EKRS
Sbjct: 466 GKEMAFLQMKRVVAGIMGRFRVVPAMVEGIEPEYTAHFTSVMKGGFPVKIEKRS 510

BLAST of Cp4.1LG03g04160 vs. ExPASy Swiss-Prot
Match: Q9FMV7 (Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.4e-97
Identity = 202/472 (42.80%), Postives = 280/472 (59.32%), Query Frame = 0

Query: 30  YPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAVFRRPGGITGVMTGNPDVVEHILKTQF 89
           Y ++G +  F  NRHR L W T++LR S + T       G   ++T NP+ VEHILKT F
Sbjct: 41  YQLIGSILSFNKNRHRLLQWYTDLLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNF 100

Query: 90  ENYSKGERLVSLMEDFLGSGIFNSDGEIWRIQRKMASYEFSTKSLRNFVTENVVIEIQTK 149
            N+ KG+    L+ D LG GIFNSDGE+W  QRK+AS+EF+ +SLR F  E +  E+Q +
Sbjct: 101 YNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNR 160

Query: 150 LIPLLEKASETEQILDFQDVFERFAFDNVCKLSFNFDPGCLGGDAT-SATEFMLAFETAA 209
           LIP+L  A +  + +DFQ+V +RFAFD VCK+S  +DP CL  D T    E + AF+ AA
Sbjct: 161 LIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWDPDCL--DLTRPVPELVKAFDVAA 220

Query: 210 TLISGRT---------------------LKKSISIVHKFVDNIIRSRMEEKTT--ENKDD 269
            + + R                      L+++I  VH  V  IIR++ +      +  D 
Sbjct: 221 EISARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLSVSEIIRAKKKSLDIGGDVSDK 280

Query: 270 KDLLSRFMADKQNSPKFLRDIAISFILAGRDTTSSALTWFFWIISTRPDIKQKILKELKT 329
           +DLLSRF+A   +  + +RD  ISFI+AGRDTTS+A+TW FW++S   D++ KIL EL  
Sbjct: 281 QDLLSRFLA-AGHGEEAVRDSVISFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDEL-- 340

Query: 330 IRSKTSNQIGETFHIDELRDMHYLQATLSETLRLYPPVPVDPKACRGDDVLPDGTVVGKN 389
            R+K S  +G     ++LR+M Y +A L E +RLYPPV  D K    DD+LPDGT + K 
Sbjct: 341 -RNKGSLGLG----FEDLREMSYTKACLCEAMRLYPPVAWDSKHAANDDILPDGTPLKKG 400

Query: 390 WFVMYHAYAMGRMESIWGKNCEEFWPERWLENGVCRGESP-------FRFPIFHAGPRTC 449
             V Y  Y MGRME +WGK+ +EF P RW E     G  P       F+FP+F AGPR C
Sbjct: 401 DKVTYFPYGMGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVC 460

Query: 450 VGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRMENGLQVMVEKR 471
           +GK+MA+ QMK +V +V+ RF++I +    P     LT  M  GL+V +++R
Sbjct: 461 IGKEMAFTQMKYVVGSVLSRFKIIPVCNNRPVFVPLLTAHMAGGLKVKIKRR 502

BLAST of Cp4.1LG03g04160 vs. ExPASy Swiss-Prot
Match: Q9SMP5 (Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 1.4e-97
Identity = 206/503 (40.95%), Postives = 301/503 (59.84%), Query Frame = 0

Query: 6   ILFITFFSVFFYLRKSRSHE-------GFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSR 65
           ++     ++ F+L  S + +       G   YP++G +  F  NRHR L W TE+LR S 
Sbjct: 8   LILAFLITIIFFLSSSSTKKVQENTTYGPPSYPLIGSILSFNKNRHRLLQWYTELLRLSP 67

Query: 66  NNTAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIW 125
           + T +    G    ++T NP  VE+ILKT F N+ KG+    L+ D LG GIFN DG  W
Sbjct: 68  SQTILVPLLGNRRTIITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNVDGHSW 127

Query: 126 RIQRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNV 185
             QRK+AS+EFST+SLR+F  E +  E++ +L+P+L  A++    +D QDV +RFAFD V
Sbjct: 128 SSQRKLASHEFSTRSLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRFAFDVV 187

Query: 186 CKLSFNFDPGCLGGDATSATEFML-AFETAATLI---------------------SGRTL 245
           CK+S  +DP CL  D T     ++ AF+TAA +                      S R L
Sbjct: 188 CKVSLGWDPDCL--DLTRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVGSERKL 247

Query: 246 KKSISIVHKFVDNIIRSRME--EKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAG 305
           +++I  VH  V  I+R++ +  E  T  +  +DLLSRF+A   N  + +RD+ ISFI+AG
Sbjct: 248 REAIRTVHVLVSEIVRAKKKSLEIGTGAEAKQDLLSRFLAAGHNG-EAVRDMVISFIMAG 307

Query: 306 RDTTSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLS 365
           RDTTS+A+TW FW+++   D+++KIL+E+  + S     +G  F  ++L++M Y +A L 
Sbjct: 308 RDTTSAAMTWLFWLLTENDDVERKILEEVDPLVS-----LGLGF--EDLKEMAYTKACLC 367

Query: 366 ETLRLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERW 425
           E +RLYPPV  D K    DDVLPDGT V +   V Y  Y MGRME++WG + EEF P RW
Sbjct: 368 EAMRLYPPVSWDSKHAANDDVLPDGTRVKRGDKVTYFPYGMGRMETLWGTDSEEFNPNRW 427

Query: 426 LEN------GVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKET 472
            ++       V +  SP++FP+F AGPR CVGK+MA++QMK +V +V+ RFE++ + K+ 
Sbjct: 428 FDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIVPVNKDR 487

BLAST of Cp4.1LG03g04160 vs. ExPASy Swiss-Prot
Match: Q9ZUX1 (Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 3.2e-94
Identity = 192/451 (42.57%), Postives = 276/451 (61.20%), Query Frame = 0

Query: 48  DWTTEVLRNSRNNTAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLG 107
           DW T +LR S  +T    +   +  V+T NP  VEHILKT F NY KG++   ++ D LG
Sbjct: 54  DWYTHLLRRSPTSTI---KVHVLNSVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLG 113

Query: 108 SGIFNSDGEIWRIQRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETE-QILDF 167
            GIFNSDG+ WR QRK+AS E  + S+R F  E V  EI+T+L+P+L   S+    +LD 
Sbjct: 114 RGIFNSDGDTWRFQRKLASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDL 173

Query: 168 QDVFERFAFDNVCKLSFNFDPGCLGGDATSATEFMLAFETAATLISGRT----------- 227
           QDVF RF+FD + KLSF FDP CL       +EF +AF+TA+ L + R            
Sbjct: 174 QDVFRRFSFDTISKLSFGFDPDCL-RLPFPISEFAVAFDTASLLSAKRALAPFPLLWKTK 233

Query: 228 ----------LKKSISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMA-DKQNSPKFLR 287
                     L++SI+++++   ++I+ R   + T      DL+SRFMA   ++  ++LR
Sbjct: 234 RLLRIGSEKKLQESINVINRLAGDLIKQR---RLTGLMGKNDLISRFMAVVAEDDDEYLR 293

Query: 288 DIAISFILAGRDTTSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELR 347
           DI +SF+LAGRDT ++ LT FFW+++  P+++ +I +EL  +     + +  T   DE+R
Sbjct: 294 DIVVSFLLAGRDTVAAGLTGFFWLLTRHPEVENRIREELDRVMGTGFDSV--TARCDEMR 353

Query: 348 DMHYLQATLSETLRLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGK 407
           +M YL A+L E++RL+PPV  D K    DDVL DGT V     V YHAYAMGRM+ IWG 
Sbjct: 354 EMDYLHASLYESMRLFPPVQFDSKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGP 413

Query: 408 NCEEFWPERWLEN-GVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVII 467
           + EEF PERWL+N G  R E+P ++P+F AG R C+GK+MA ++MK+I  A++ RFE  +
Sbjct: 414 DYEEFKPERWLDNEGKFRPENPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRV 473

Query: 468 MKKETPKHYQ---SLTLRMENGLQVMVEKRS 472
              ET +  +    LT  +  GL VM+++RS
Sbjct: 474 ASPETTETLRFAPGLTATVNGGLPVMIQERS 495

BLAST of Cp4.1LG03g04160 vs. NCBI nr
Match: XP_023528952.1 (cytochrome P450 94A1-like [Cucurbita pepo subsp. pepo] >XP_023528965.1 cytochrome P450 94A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 939 bits (2428), Expect = 0.0
Identity = 472/493 (95.74%), Postives = 472/493 (95.74%), Query Frame = 0

Query: 1   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 60
           VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN
Sbjct: 3   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 62

Query: 61  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 120
           TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI
Sbjct: 63  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 122

Query: 121 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 180
           QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK
Sbjct: 123 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 182

Query: 181 LSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKS 240
           LSFNFDPGCLGGDATSATEFMLAFETAATLISGR                     TLKKS
Sbjct: 183 LSFNFDPGCLGGDATSATEFMLAFETAATLISGRFRYAIPKWYKIKKFFNLGSERTLKKS 242

Query: 241 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 300
           ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS
Sbjct: 243 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 302

Query: 301 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 360
           SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL
Sbjct: 303 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 362

Query: 361 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 420
           YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV
Sbjct: 363 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 422

Query: 421 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 472
           CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM
Sbjct: 423 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 482

BLAST of Cp4.1LG03g04160 vs. NCBI nr
Match: XP_023522613.1 (cytochrome P450 94A1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 937 bits (2423), Expect = 0.0
Identity = 471/493 (95.54%), Postives = 471/493 (95.54%), Query Frame = 0

Query: 1   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 60
           VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN
Sbjct: 3   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 62

Query: 61  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 120
           TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI
Sbjct: 63  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 122

Query: 121 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 180
           QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK
Sbjct: 123 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 182

Query: 181 LSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKS 240
           LSFNFDPGCLGGDATSATEFMLAFETAATLISGR                     TLKKS
Sbjct: 183 LSFNFDPGCLGGDATSATEFMLAFETAATLISGRFRYAIPKWYKIKKFFNLGSERTLKKS 242

Query: 241 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 300
           ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS
Sbjct: 243 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 302

Query: 301 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 360
           SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL
Sbjct: 303 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 362

Query: 361 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 420
           YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV
Sbjct: 363 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 422

Query: 421 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 472
           CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHY SLTLRM
Sbjct: 423 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYHSLTLRM 482

BLAST of Cp4.1LG03g04160 vs. NCBI nr
Match: KAG7017629.1 (Cytochrome P450 94A1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 904 bits (2335), Expect = 0.0
Identity = 453/493 (91.89%), Postives = 463/493 (93.91%), Query Frame = 0

Query: 1   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 60
           + V TILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN
Sbjct: 3   ISVVTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 62

Query: 61  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 120
           TAVFRRPGGI+GVMT NPDVVEHILKTQFENYSKGERLVS++EDFLGSGIFNSDGEIWR 
Sbjct: 63  TAVFRRPGGISGVMTANPDVVEHILKTQFENYSKGERLVSVLEDFLGSGIFNSDGEIWRF 122

Query: 121 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 180
           QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK
Sbjct: 123 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 182

Query: 181 LSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKS 240
           LSFNFDPGCLGGD TS T+FMLAFETA+TLISGR                     TLKKS
Sbjct: 183 LSFNFDPGCLGGDGTSGTDFMLAFETASTLISGRFRYAIPKWYKIKKFFNLGSERTLKKS 242

Query: 241 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 300
           ISIVHKFVDNIIRSR EEKTTENKDD+DLLSRFMADKQNSPKFLRDIAISFILAGRDTTS
Sbjct: 243 ISIVHKFVDNIIRSRTEEKTTENKDDEDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 302

Query: 301 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 360
           SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQI ETFHI+ELRDMHYLQATLSETLRL
Sbjct: 303 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIRETFHINELRDMHYLQATLSETLRL 362

Query: 361 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 420
           YPPVPVD KAC+GDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV
Sbjct: 363 YPPVPVDSKACQGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 422

Query: 421 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 472
           CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM
Sbjct: 423 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 482

BLAST of Cp4.1LG03g04160 vs. NCBI nr
Match: KAG6580873.1 (Cytochrome P450 94A1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 450/493 (91.28%), Postives = 460/493 (93.31%), Query Frame = 0

Query: 1   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 60
           + V TILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN
Sbjct: 3   ISVVTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 62

Query: 61  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 120
           TAVFRRPGGI+GVMT NPDVVEHILKTQFENYSKGERLVS++EDFLGSGIFNSDGEIWR 
Sbjct: 63  TAVFRRPGGISGVMTANPDVVEHILKTQFENYSKGERLVSVLEDFLGSGIFNSDGEIWRF 122

Query: 121 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 180
           QRKMASYEFSTKSLRNFVTENV IEIQ KLIPLLEKASETEQILDFQDVFERFAFDNVCK
Sbjct: 123 QRKMASYEFSTKSLRNFVTENVAIEIQMKLIPLLEKASETEQILDFQDVFERFAFDNVCK 182

Query: 181 LSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKS 240
           LSFNFDPGCL GD TS T+FMLAFETA+TLISGR                     TLKKS
Sbjct: 183 LSFNFDPGCLDGDGTSGTDFMLAFETASTLISGRFRYAIPKWYKIKKFFNLGSERTLKKS 242

Query: 241 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 300
           ISIVHKFVDNIIRSR EEKTTENKDD+DLLSRFMADKQNSPKFLRDIAISFILAGRDTTS
Sbjct: 243 ISIVHKFVDNIIRSRTEEKTTENKDDEDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 302

Query: 301 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 360
           SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQI ETFHI+ELRDMHYLQATLSETLRL
Sbjct: 303 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIRETFHINELRDMHYLQATLSETLRL 362

Query: 361 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 420
           YPPVPVD KAC+GDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV
Sbjct: 363 YPPVPVDSKACQGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 422

Query: 421 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 472
           CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM
Sbjct: 423 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 482

BLAST of Cp4.1LG03g04160 vs. NCBI nr
Match: XP_022935257.1 (cytochrome P450 94A1-like [Cucurbita moschata])

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 448/493 (90.87%), Postives = 459/493 (93.10%), Query Frame = 0

Query: 1   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 60
           + + TILFIT FS+FFYLRKSRSHEGF+LYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN
Sbjct: 5   ISIATILFITIFSLFFYLRKSRSHEGFKLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 64

Query: 61  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 120
           TAVFRRPGGI+GVMT NPDVVEHILKTQFENYSKGERLVS++EDFLGSGIFNSDGEIWR 
Sbjct: 65  TAVFRRPGGISGVMTANPDVVEHILKTQFENYSKGERLVSVLEDFLGSGIFNSDGEIWRF 124

Query: 121 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 180
           QRKMASYEFSTKSLRNFVTENVVIEIQ KLIPLLEKASETEQILDFQDVFERFAFDNVCK
Sbjct: 125 QRKMASYEFSTKSLRNFVTENVVIEIQMKLIPLLEKASETEQILDFQDVFERFAFDNVCK 184

Query: 181 LSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKS 240
           LSFNFDPGCLGGD TS T+FMLAFETA+TLISGR                     TLKKS
Sbjct: 185 LSFNFDPGCLGGDGTSGTDFMLAFETASTLISGRFRYAIPKWYNIKKFFNLGSERTLKKS 244

Query: 241 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 300
           ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS
Sbjct: 245 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 304

Query: 301 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 360
           SALTWFFWIISTRPDIKQKIL ELKTIRSKTSNQI ETFHI+ELRDMHYLQATLSETLRL
Sbjct: 305 SALTWFFWIISTRPDIKQKILAELKTIRSKTSNQIRETFHINELRDMHYLQATLSETLRL 364

Query: 361 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 420
           YPPVPVD KACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV
Sbjct: 365 YPPVPVDSKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 424

Query: 421 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 472
           CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETP H QSLTLRM
Sbjct: 425 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPNHLQSLTLRM 484

BLAST of Cp4.1LG03g04160 vs. ExPASy TrEMBL
Match: A0A6J1F525 (cytochrome P450 94A1-like OS=Cucurbita moschata OX=3662 GN=LOC111442194 PE=3 SV=1)

HSP 1 Score: 895 bits (2314), Expect = 0.0
Identity = 448/493 (90.87%), Postives = 459/493 (93.10%), Query Frame = 0

Query: 1   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 60
           + + TILFIT FS+FFYLRKSRSHEGF+LYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN
Sbjct: 5   ISIATILFITIFSLFFYLRKSRSHEGFKLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 64

Query: 61  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 120
           TAVFRRPGGI+GVMT NPDVVEHILKTQFENYSKGERLVS++EDFLGSGIFNSDGEIWR 
Sbjct: 65  TAVFRRPGGISGVMTANPDVVEHILKTQFENYSKGERLVSVLEDFLGSGIFNSDGEIWRF 124

Query: 121 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 180
           QRKMASYEFSTKSLRNFVTENVVIEIQ KLIPLLEKASETEQILDFQDVFERFAFDNVCK
Sbjct: 125 QRKMASYEFSTKSLRNFVTENVVIEIQMKLIPLLEKASETEQILDFQDVFERFAFDNVCK 184

Query: 181 LSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKS 240
           LSFNFDPGCLGGD TS T+FMLAFETA+TLISGR                     TLKKS
Sbjct: 185 LSFNFDPGCLGGDGTSGTDFMLAFETASTLISGRFRYAIPKWYNIKKFFNLGSERTLKKS 244

Query: 241 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 300
           ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS
Sbjct: 245 ISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTS 304

Query: 301 SALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRL 360
           SALTWFFWIISTRPDIKQKIL ELKTIRSKTSNQI ETFHI+ELRDMHYLQATLSETLRL
Sbjct: 305 SALTWFFWIISTRPDIKQKILAELKTIRSKTSNQIRETFHINELRDMHYLQATLSETLRL 364

Query: 361 YPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 420
           YPPVPVD KACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV
Sbjct: 365 YPPVPVDSKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGV 424

Query: 421 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRM 472
           CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETP H QSLTLRM
Sbjct: 425 CRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPNHLQSLTLRM 484

BLAST of Cp4.1LG03g04160 vs. ExPASy TrEMBL
Match: A0A6J1J8E1 (cytochrome P450 94A1-like OS=Cucurbita maxima OX=3661 GN=LOC111482283 PE=3 SV=1)

HSP 1 Score: 872 bits (2254), Expect = 0.0
Identity = 439/490 (89.59%), Postives = 451/490 (92.04%), Query Frame = 0

Query: 4   GTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAV 63
           GTILF TFFS+FFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAV
Sbjct: 6   GTILFFTFFSLFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAV 65

Query: 64  FRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRIQRK 123
           FRRPGGI+GVMT NPDVVEHILKTQFENYSKGERLVSL+EDFLGSGIFNSDGEIWRIQRK
Sbjct: 66  FRRPGGISGVMTANPDVVEHILKTQFENYSKGERLVSLLEDFLGSGIFNSDGEIWRIQRK 125

Query: 124 MASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCKLSF 183
           MASYEFSTKSLR FVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCKLSF
Sbjct: 126 MASYEFSTKSLRKFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCKLSF 185

Query: 184 NFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKSISI 243
           +FDP CLGGD TS TEFMLAFETAATLISGR                     TLKKSIS 
Sbjct: 186 SFDPSCLGGDGTSGTEFMLAFETAATLISGRFRYAIPKWYKIKRFFNLGSERTLKKSIST 245

Query: 244 VHKFVDNIIRSRMEEKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDTTSSAL 303
           VH FVDNIIRSRMEEKTTENKD KDLLSRFMADK+NSPKFLRDI ISFILAGRDTTSSAL
Sbjct: 246 VHNFVDNIIRSRMEEKTTENKD-KDLLSRFMADKENSPKFLRDIVISFILAGRDTTSSAL 305

Query: 304 TWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRLYPP 363
           TWFFWIISTRPDIKQKILKELKTIRSKT NQIGETFHI+ELRDMHYLQATLSETLRLYPP
Sbjct: 306 TWFFWIISTRPDIKQKILKELKTIRSKTGNQIGETFHINELRDMHYLQATLSETLRLYPP 365

Query: 364 VPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLENGVCRG 423
           VPVD KACR DDVLPDGTVVGKNWFVMYHAYAMG+MESIWGKNCEEF PERWLENGVCRG
Sbjct: 366 VPVDSKACRRDDVLPDGTVVGKNWFVMYHAYAMGKMESIWGKNCEEFRPERWLENGVCRG 425

Query: 424 ESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRMENG 472
           ESPFRFPIFHAGPRTCVGKDMAY+QMKAI AAV+ERFEVIIMK +TP H QSLTLRMENG
Sbjct: 426 ESPFRFPIFHAGPRTCVGKDMAYIQMKAIAAAVIERFEVIIMKNKTPNHLQSLTLRMENG 485

BLAST of Cp4.1LG03g04160 vs. ExPASy TrEMBL
Match: A0A5D3DPN2 (Cytochrome P450 94A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006500 PE=3 SV=1)

HSP 1 Score: 697 bits (1798), Expect = 1.39e-248
Identity = 344/494 (69.64%), Postives = 404/494 (81.78%), Query Frame = 0

Query: 7   LFITFFSVFFYL-------RKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRN 66
           +F+TFFS   Y         KS+  +GF+ +P VG LPLFLLNRHRFLDW+TEVLRN R 
Sbjct: 12  IFLTFFSFSIYFLFFTSTKSKSKPQQGFKHFPFVGTLPLFLLNRHRFLDWSTEVLRNCRT 71

Query: 67  NTAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWR 126
           NTAVF+RPG + GV+T NP VVEHILKTQFENY KGER +SL+EDFLG GIFNSDGEIW+
Sbjct: 72  NTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNSDGEIWK 131

Query: 127 IQRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVC 186
           +QRK ASYEF+TKSLRNFV ENV +EIQ++L+P+  KA ETE+ILD QDV ERFAFDNVC
Sbjct: 132 VQRKTASYEFNTKSLRNFVMENVRVEIQSRLLPIFGKACETERILDLQDVLERFAFDNVC 191

Query: 187 KLSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKK 246
           KL+FN+DP CLGGD T+ATEFM AFE AATL SGR                     TLK+
Sbjct: 192 KLAFNYDPACLGGDGTAATEFMRAFEHAATLSSGRFMYAFPGSYKVKKFFNIGSEKTLKE 251

Query: 247 SISIVHKFVDNIIRSRMEEK-TTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDT 306
           SI++VHKF ++II SR+EEK TT+ ++D+DLLSRFM D+ NSP+FLRDI ISFILAGRDT
Sbjct: 252 SIAVVHKFAEDIIHSRLEEKKTTQIENDQDLLSRFMGDQNNSPQFLRDIIISFILAGRDT 311

Query: 307 TSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETL 366
           TSSALTWFFWI+S+RPD++QKIL EL+TIR+K   +IGE +  DELRDMHYLQA LSETL
Sbjct: 312 TSSALTWFFWILSSRPDVEQKILAELETIRTKAHKEIGEMYSFDELRDMHYLQAALSETL 371

Query: 367 RLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLEN 426
           RLYPPVPVD KACR DDVLPDGT +GK+WFV YH YAMGRMESIWGK+  EF PERWLEN
Sbjct: 372 RLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKDYGEFSPERWLEN 431

Query: 427 GVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEV-IIMKKETPKHYQSLT 470
           GVC+ ESPFRFPIFHAGPR C+GKDMAY+QMK+I AAV+E+FEV ++ KK++PKH  SLT
Sbjct: 432 GVCKTESPFRFPIFHAGPRMCLGKDMAYIQMKSIAAAVIEKFEVEMVEKKKSPKHLLSLT 491

BLAST of Cp4.1LG03g04160 vs. ExPASy TrEMBL
Match: A0A1S3B728 (cytochrome P450 94A1-like OS=Cucumis melo OX=3656 GN=LOC103486871 PE=3 SV=1)

HSP 1 Score: 697 bits (1798), Expect = 1.39e-248
Identity = 344/494 (69.64%), Postives = 404/494 (81.78%), Query Frame = 0

Query: 7   LFITFFSVFFYL-------RKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRN 66
           +F+TFFS   Y         KS+  +GF+ +P VG LPLFLLNRHRFLDW+TEVLRN R 
Sbjct: 12  IFLTFFSFSIYFLFFTSTKSKSKPQQGFKHFPFVGTLPLFLLNRHRFLDWSTEVLRNCRT 71

Query: 67  NTAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWR 126
           NTAVF+RPG + GV+T NP VVEHILKTQFENY KGER +SL+EDFLG GIFNSDGEIW+
Sbjct: 72  NTAVFKRPGKVHGVITANPLVVEHILKTQFENYPKGERFISLLEDFLGRGIFNSDGEIWK 131

Query: 127 IQRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVC 186
           +QRK ASYEF+TKSLRNFV ENV +EIQ++L+P+  KA ETE+ILD QDV ERFAFDNVC
Sbjct: 132 VQRKTASYEFNTKSLRNFVMENVRVEIQSRLLPIFGKACETERILDLQDVLERFAFDNVC 191

Query: 187 KLSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKK 246
           KL+FN+DP CLGGD T+ATEFM AFE AATL SGR                     TLK+
Sbjct: 192 KLAFNYDPACLGGDGTAATEFMRAFEHAATLSSGRFMYAFPGSYKVKKFFNIGSEKTLKE 251

Query: 247 SISIVHKFVDNIIRSRMEEK-TTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAGRDT 306
           SI++VHKF ++II SR+EEK TT+ ++D+DLLSRFM D+ NSP+FLRDI ISFILAGRDT
Sbjct: 252 SIAVVHKFAEDIIHSRLEEKKTTQIENDQDLLSRFMGDQNNSPQFLRDIIISFILAGRDT 311

Query: 307 TSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETL 366
           TSSALTWFFWI+S+RPD++QKIL EL+TIR+K   +IGE +  DELRDMHYLQA LSETL
Sbjct: 312 TSSALTWFFWILSSRPDVEQKILAELETIRTKAHKEIGEMYSFDELRDMHYLQAALSETL 371

Query: 367 RLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLEN 426
           RLYPPVPVD KACR DDVLPDGT +GK+WFV YH YAMGRMESIWGK+  EF PERWLEN
Sbjct: 372 RLYPPVPVDTKACRNDDVLPDGTFIGKSWFVTYHTYAMGRMESIWGKDYGEFSPERWLEN 431

Query: 427 GVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEV-IIMKKETPKHYQSLT 470
           GVC+ ESPFRFPIFHAGPR C+GKDMAY+QMK+I AAV+E+FEV ++ KK++PKH  SLT
Sbjct: 432 GVCKTESPFRFPIFHAGPRMCLGKDMAYIQMKSIAAAVIEKFEVEMVEKKKSPKHLLSLT 491

BLAST of Cp4.1LG03g04160 vs. ExPASy TrEMBL
Match: A0A6J1EZ74 (cytochrome P450 94A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440896 PE=3 SV=1)

HSP 1 Score: 686 bits (1770), Expect = 2.26e-244
Identity = 342/494 (69.23%), Postives = 404/494 (81.78%), Query Frame = 0

Query: 7   LFITFFSVFFYL------RKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 66
           LF+TF S+  YL      +KS+S++GF+ +P+VG LPLFLLNRHRFLDW+TEVL N + N
Sbjct: 9   LFLTFLSLCLYLLLFASPKKSKSNQGFKHFPIVGTLPLFLLNRHRFLDWSTEVLSNCQTN 68

Query: 67  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 126
           TAVFRRPG + GV+T NP VVEHILKTQFENY KG+R +SL+EDFLG GIFNSDGEIW++
Sbjct: 69  TAVFRRPGKVHGVITANPLVVEHILKTQFENYPKGKRFISLLEDFLGRGIFNSDGEIWKV 128

Query: 127 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 186
           QRK ASYEF+TKSLRNFV ENV +EIQT+L+P+L KASETE+ILD QDV ERFAFDNVCK
Sbjct: 129 QRKTASYEFNTKSLRNFVMENVTVEIQTRLLPILGKASETERILDLQDVLERFAFDNVCK 188

Query: 187 LSFNFDPGCLGGDATSATEFMLAFETAATLISGR---------------------TLKKS 246
           L+FNFDP CLGGD TS  +FM AFE AATL SGR                     TLKKS
Sbjct: 189 LAFNFDPCCLGGDGTSGADFMRAFEDAATLSSGRFMYALPGLYKVKKFLNMGSERTLKKS 248

Query: 247 ISIVHKFVDNIIRSRMEEK-TTENKDDKDLLSRFMADKQN-SPKFLRDIAISFILAGRDT 306
           I+ VHKF ++IIRSRMEEK TT  K+D+DLLSRFM  ++N SP+FLRDI ISFILAGRDT
Sbjct: 249 IARVHKFAEDIIRSRMEEKKTTHVKNDQDLLSRFMGSQENNSPEFLRDIIISFILAGRDT 308

Query: 307 TSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETL 366
           TSSALTWFFWI+S+RP+I QKILKEL+TIRS+T  ++G  +  +ELRDMHYLQA LSETL
Sbjct: 309 TSSALTWFFWILSSRPNITQKILKELETIRSRTGKKLGAEYSFEELRDMHYLQAALSETL 368

Query: 367 RLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLEN 426
           RLYPPVPVD KACR +DVLPDGT+VG+ WFV YH YAMGRME IWG+NC EF PERW+EN
Sbjct: 369 RLYPPVPVDTKACRNEDVLPDGTLVGREWFVTYHTYAMGRMERIWGENCGEFLPERWVEN 428

Query: 427 GVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKE-TPKHYQSLT 470
           GVCR ESPFRFP+FHAGPR C+GK+MAY+QMK+I AAV+ERFEV + +KE +PK+  SLT
Sbjct: 429 GVCRAESPFRFPVFHAGPRMCLGKEMAYIQMKSIAAAVIERFEVGMAEKEKSPKYLLSLT 488

BLAST of Cp4.1LG03g04160 vs. TAIR 10
Match: AT3G56630.1 (cytochrome P450, family 94, subfamily D, polypeptide 2 )

HSP 1 Score: 549.3 bits (1414), Expect = 3.1e-156
Identity = 274/497 (55.13%), Postives = 363/497 (73.04%), Query Frame = 0

Query: 1   VCVGTILFITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNN 60
           +C   ++F  FF      + S S  GF+ YP+VG LP  + NRHRFLDWT E L      
Sbjct: 10  LCFPIVIFFIFFP-----KNSSSEFGFKSYPIVGSLPGLVNNRHRFLDWTVETLSRCPTQ 69

Query: 61  TAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRI 120
           TA+FRRPG +  VMT NP  VE++LKT+FE++ KGER +S++EDFLG GIFNSDGE+W  
Sbjct: 70  TAIFRRPGKLQFVMTANPANVEYMLKTKFESFPKGERFISILEDFLGRGIFNSDGEMWWK 129

Query: 121 QRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCK 180
           QRK ASYEFSTKSLR+FV  NV +EI T+L+P+L +A+   +++D QD+ ERFAFDN+CK
Sbjct: 130 QRKTASYEFSTKSLRDFVMSNVTVEINTRLVPVLAEAATNGKLIDLQDILERFAFDNICK 189

Query: 181 LSFNFDPGCLGGDATSATEFMLAFETAATLI---------------------SGRTLKKS 240
           L+FN D  CLG D  +   FM AFETAAT+I                     S R L++S
Sbjct: 190 LAFNVDSACLGDDGAAGVNFMQAFETAATIISQRFQSVISYSWKIKKKLNIGSERVLRES 249

Query: 241 ISIVHKFVDNIIRSRMEE-KTTENKDDKDLLSRFMA-DKQNSPKFLRDIAISFILAGRDT 300
           I IVHKF D I+R+R+E+ K +++K+  DLLSRF++ ++ NSP+ LRDI ISFILAGRDT
Sbjct: 250 IMIVHKFADEIVRNRIEQGKVSDHKE--DLLSRFISKEEMNSPEILRDIVISFILAGRDT 309

Query: 301 TSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETL 360
           TSSAL+WFFW++S  P++K KIL+EL +IR +T  +IGE +  ++L+ M+YL A ++E+L
Sbjct: 310 TSSALSWFFWLLSMHPEVKDKILQELNSIRERTGKRIGEVYGFEDLKLMNYLHAAITESL 369

Query: 361 RLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLE- 420
           RLYPPVPVD  +C  D+VLPDGT +GK+W + Y+AYAMGRMESIWGK+C+ F PERW++ 
Sbjct: 370 RLYPPVPVDTMSCAEDNVLPDGTFIGKDWGISYNAYAMGRMESIWGKDCDRFDPERWIDE 429

Query: 421 -NGVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIM-KKETPKHYQS 472
            NG  RGE+P++FP FHAGPR C+GK+MAY+QMK+IVAAV+ERF V +  KKE P+   S
Sbjct: 430 TNGGFRGENPYKFPAFHAGPRMCLGKEMAYIQMKSIVAAVLERFVVEVPGKKERPEILMS 489

BLAST of Cp4.1LG03g04160 vs. TAIR 10
Match: AT1G34540.1 (cytochrome P450, family 94, subfamily D, polypeptide 1 )

HSP 1 Score: 535.4 bits (1378), Expect = 4.6e-152
Identity = 267/488 (54.71%), Postives = 357/488 (73.16%), Query Frame = 0

Query: 9   ITFFSVFFYLRKSRSHEGFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAVFRRPG 68
           I+ F +FF  +KS S  GF+ YP+VG  P  + NRHRFLDWT E L      TA+FRRPG
Sbjct: 14  ISIFFIFF-TKKSSSEFGFKSYPIVGSFPGLVNNRHRFLDWTVETLSRCPTQTAIFRRPG 73

Query: 69  GITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIWRIQRKMASYE 128
               +MT NP  VE++LKT+FE++ KG++  S++EDFLG GIFNSDG++W  QRK ASYE
Sbjct: 74  KQQLIMTANPSNVEYMLKTKFESFPKGQQFTSVLEDFLGHGIFNSDGDMWWKQRKTASYE 133

Query: 129 FSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNVCKLSFNFDPG 188
           FSTKSLR+FV  NV +EI T+L+P+L +A+ T +++D QD+ ERFAFDN+CKL+FN D  
Sbjct: 134 FSTKSLRDFVMSNVTVEINTRLVPVLVEAATTGKLIDLQDILERFAFDNICKLAFNVDCA 193

Query: 189 CLGGDATSATEFMLAFETAATLI---------------------SGRTLKKSISIVHKFV 248
           CLG D      FM AFETAAT+I                     S R L++SI+ VHKF 
Sbjct: 194 CLGHDGAVGVNFMRAFETAATIISQRFRSVASCAWRIKKKLNIGSERVLRESIATVHKFA 253

Query: 249 DNIIRSRMEE-KTTENKDDKDLLSRFMA-DKQNSPKFLRDIAISFILAGRDTTSSALTWF 308
           D I+R+R+++ +++++K+  DLLSRF++ ++ NSP+ LRDI ISFILAGRDTTSSAL+WF
Sbjct: 254 DEIVRNRIDQGRSSDHKE--DLLSRFISKEEMNSPEILRDIVISFILAGRDTTSSALSWF 313

Query: 309 FWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLSETLRLYPPVPV 368
           FW++S  P+++ KIL+EL +IR++T  +IGE +  + L+ M+YL A ++E+LRLYPPVPV
Sbjct: 314 FWLLSMHPEVEDKILQELNSIRARTGKRIGEVYGFEHLKMMNYLHAAITESLRLYPPVPV 373

Query: 369 DPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERWLE--NGVCRGE 428
           D K+C  D+VLPDGT VGK W + Y+ +AMGRMESIWGK+C+ F PERW++  NG  RGE
Sbjct: 374 DIKSCAEDNVLPDGTFVGKGWAITYNIFAMGRMESIWGKDCDRFDPERWIDETNGCFRGE 433

Query: 429 SPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRMENGL 472
            P +FP FHAGPR CVGKDMAY+QMK+IVAAV+ERF V +  KE P+   S+TLR++ GL
Sbjct: 434 DPSKFPAFHAGPRMCVGKDMAYIQMKSIVAAVLERFVVEVPGKERPEILLSMTLRIKGGL 493

BLAST of Cp4.1LG03g04160 vs. TAIR 10
Match: AT3G48520.1 (cytochrome P450, family 94, subfamily B, polypeptide 3 )

HSP 1 Score: 358.2 bits (918), Expect = 1.0e-98
Identity = 206/503 (40.95%), Postives = 301/503 (59.84%), Query Frame = 0

Query: 6   ILFITFFSVFFYLRKSRSHE-------GFRLYPVVGVLPLFLLNRHRFLDWTTEVLRNSR 65
           ++     ++ F+L  S + +       G   YP++G +  F  NRHR L W TE+LR S 
Sbjct: 8   LILAFLITIIFFLSSSSTKKVQENTTYGPPSYPLIGSILSFNKNRHRLLQWYTELLRLSP 67

Query: 66  NNTAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLGSGIFNSDGEIW 125
           + T +    G    ++T NP  VE+ILKT F N+ KG+    L+ D LG GIFN DG  W
Sbjct: 68  SQTILVPLLGNRRTIITTNPLNVEYILKTNFFNFPKGKPFTDLLGDLLGGGIFNVDGHSW 127

Query: 126 RIQRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETEQILDFQDVFERFAFDNV 185
             QRK+AS+EFST+SLR+F  E +  E++ +L+P+L  A++    +D QDV +RFAFD V
Sbjct: 128 SSQRKLASHEFSTRSLRSFAFEVLKDEVENRLVPVLSTAADVGTTVDLQDVLKRFAFDVV 187

Query: 186 CKLSFNFDPGCLGGDATSATEFML-AFETAATLI---------------------SGRTL 245
           CK+S  +DP CL  D T     ++ AF+TAA +                      S R L
Sbjct: 188 CKVSLGWDPDCL--DLTRPVNPLVEAFDTAAEISARRATEPIYAVWKTKRVLNVGSERKL 247

Query: 246 KKSISIVHKFVDNIIRSRME--EKTTENKDDKDLLSRFMADKQNSPKFLRDIAISFILAG 305
           +++I  VH  V  I+R++ +  E  T  +  +DLLSRF+A   N  + +RD+ ISFI+AG
Sbjct: 248 REAIRTVHVLVSEIVRAKKKSLEIGTGAEAKQDLLSRFLAAGHNG-EAVRDMVISFIMAG 307

Query: 306 RDTTSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELRDMHYLQATLS 365
           RDTTS+A+TW FW+++   D+++KIL+E+  + S     +G  F  ++L++M Y +A L 
Sbjct: 308 RDTTSAAMTWLFWLLTENDDVERKILEEVDPLVS-----LGLGF--EDLKEMAYTKACLC 367

Query: 366 ETLRLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGKNCEEFWPERW 425
           E +RLYPPV  D K    DDVLPDGT V +   V Y  Y MGRME++WG + EEF P RW
Sbjct: 368 EAMRLYPPVSWDSKHAANDDVLPDGTRVKRGDKVTYFPYGMGRMETLWGTDSEEFNPNRW 427

Query: 426 LEN------GVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVIIMKKET 472
            ++       V +  SP++FP+F AGPR CVGK+MA++QMK +V +V+ RFE++ + K+ 
Sbjct: 428 FDSEPGSTRPVLKPISPYKFPVFQAGPRVCVGKEMAFMQMKYVVGSVLSRFEIVPVNKDR 487

BLAST of Cp4.1LG03g04160 vs. TAIR 10
Match: AT5G63450.1 (cytochrome P450, family 94, subfamily B, polypeptide 1 )

HSP 1 Score: 358.2 bits (918), Expect = 1.0e-98
Identity = 202/472 (42.80%), Postives = 280/472 (59.32%), Query Frame = 0

Query: 30  YPVVGVLPLFLLNRHRFLDWTTEVLRNSRNNTAVFRRPGGITGVMTGNPDVVEHILKTQF 89
           Y ++G +  F  NRHR L W T++LR S + T       G   ++T NP+ VEHILKT F
Sbjct: 41  YQLIGSILSFNKNRHRLLQWYTDLLRLSPSQTITVDLLFGRRTIITANPENVEHILKTNF 100

Query: 90  ENYSKGERLVSLMEDFLGSGIFNSDGEIWRIQRKMASYEFSTKSLRNFVTENVVIEIQTK 149
            N+ KG+    L+ D LG GIFNSDGE+W  QRK+AS+EF+ +SLR F  E +  E+Q +
Sbjct: 101 YNFPKGKPFTDLLGDLLGGGIFNSDGELWSSQRKLASHEFTMRSLREFTFEILREEVQNR 160

Query: 150 LIPLLEKASETEQILDFQDVFERFAFDNVCKLSFNFDPGCLGGDAT-SATEFMLAFETAA 209
           LIP+L  A +  + +DFQ+V +RFAFD VCK+S  +DP CL  D T    E + AF+ AA
Sbjct: 161 LIPVLSSAVDCGETVDFQEVLKRFAFDVVCKVSLGWDPDCL--DLTRPVPELVKAFDVAA 220

Query: 210 TLISGRT---------------------LKKSISIVHKFVDNIIRSRMEEKTT--ENKDD 269
            + + R                      L+++I  VH  V  IIR++ +      +  D 
Sbjct: 221 EISARRATEPVYAVWKVKRFLNVGSEKRLREAIKTVHLSVSEIIRAKKKSLDIGGDVSDK 280

Query: 270 KDLLSRFMADKQNSPKFLRDIAISFILAGRDTTSSALTWFFWIISTRPDIKQKILKELKT 329
           +DLLSRF+A   +  + +RD  ISFI+AGRDTTS+A+TW FW++S   D++ KIL EL  
Sbjct: 281 QDLLSRFLA-AGHGEEAVRDSVISFIMAGRDTTSAAMTWLFWLLSQNDDVETKILDEL-- 340

Query: 330 IRSKTSNQIGETFHIDELRDMHYLQATLSETLRLYPPVPVDPKACRGDDVLPDGTVVGKN 389
            R+K S  +G     ++LR+M Y +A L E +RLYPPV  D K    DD+LPDGT + K 
Sbjct: 341 -RNKGSLGLG----FEDLREMSYTKACLCEAMRLYPPVAWDSKHAANDDILPDGTPLKKG 400

Query: 390 WFVMYHAYAMGRMESIWGKNCEEFWPERWLENGVCRGESP-------FRFPIFHAGPRTC 449
             V Y  Y MGRME +WGK+ +EF P RW E     G  P       F+FP+F AGPR C
Sbjct: 401 DKVTYFPYGMGRMEKVWGKDWDEFKPNRWFEEEPSYGTKPVLKSVSSFKFPVFQAGPRVC 460

Query: 450 VGKDMAYVQMKAIVAAVMERFEVIIMKKETPKHYQSLTLRMENGLQVMVEKR 471
           +GK+MA+ QMK +V +V+ RF++I +    P     LT  M  GL+V +++R
Sbjct: 461 IGKEMAFTQMKYVVGSVLSRFKIIPVCNNRPVFVPLLTAHMAGGLKVKIKRR 502

BLAST of Cp4.1LG03g04160 vs. TAIR 10
Match: AT2G27690.1 (cytochrome P450, family 94, subfamily C, polypeptide 1 )

HSP 1 Score: 347.1 bits (889), Expect = 2.3e-95
Identity = 192/451 (42.57%), Postives = 276/451 (61.20%), Query Frame = 0

Query: 48  DWTTEVLRNSRNNTAVFRRPGGITGVMTGNPDVVEHILKTQFENYSKGERLVSLMEDFLG 107
           DW T +LR S  +T    +   +  V+T NP  VEHILKT F NY KG++   ++ D LG
Sbjct: 54  DWYTHLLRRSPTSTI---KVHVLNSVITANPSNVEHILKTNFHNYPKGKQFSVILGDLLG 113

Query: 108 SGIFNSDGEIWRIQRKMASYEFSTKSLRNFVTENVVIEIQTKLIPLLEKASETE-QILDF 167
            GIFNSDG+ WR QRK+AS E  + S+R F  E V  EI+T+L+P+L   S+    +LD 
Sbjct: 114 RGIFNSDGDTWRFQRKLASLELGSVSVRVFAHEIVKTEIETRLLPILTSFSDNPGSVLDL 173

Query: 168 QDVFERFAFDNVCKLSFNFDPGCLGGDATSATEFMLAFETAATLISGRT----------- 227
           QDVF RF+FD + KLSF FDP CL       +EF +AF+TA+ L + R            
Sbjct: 174 QDVFRRFSFDTISKLSFGFDPDCL-RLPFPISEFAVAFDTASLLSAKRALAPFPLLWKTK 233

Query: 228 ----------LKKSISIVHKFVDNIIRSRMEEKTTENKDDKDLLSRFMA-DKQNSPKFLR 287
                     L++SI+++++   ++I+ R   + T      DL+SRFMA   ++  ++LR
Sbjct: 234 RLLRIGSEKKLQESINVINRLAGDLIKQR---RLTGLMGKNDLISRFMAVVAEDDDEYLR 293

Query: 288 DIAISFILAGRDTTSSALTWFFWIISTRPDIKQKILKELKTIRSKTSNQIGETFHIDELR 347
           DI +SF+LAGRDT ++ LT FFW+++  P+++ +I +EL  +     + +  T   DE+R
Sbjct: 294 DIVVSFLLAGRDTVAAGLTGFFWLLTRHPEVENRIREELDRVMGTGFDSV--TARCDEMR 353

Query: 348 DMHYLQATLSETLRLYPPVPVDPKACRGDDVLPDGTVVGKNWFVMYHAYAMGRMESIWGK 407
           +M YL A+L E++RL+PPV  D K    DDVL DGT V     V YHAYAMGRM+ IWG 
Sbjct: 354 EMDYLHASLYESMRLFPPVQFDSKFALNDDVLSDGTFVNSGTRVTYHAYAMGRMDRIWGP 413

Query: 408 NCEEFWPERWLEN-GVCRGESPFRFPIFHAGPRTCVGKDMAYVQMKAIVAAVMERFEVII 467
           + EEF PERWL+N G  R E+P ++P+F AG R C+GK+MA ++MK+I  A++ RFE  +
Sbjct: 414 DYEEFKPERWLDNEGKFRPENPVKYPVFQAGARVCIGKEMAIMEMKSIAVAIIRRFETRV 473

Query: 468 MKKETPKHYQ---SLTLRMENGLQVMVEKRS 472
              ET +  +    LT  +  GL VM+++RS
Sbjct: 474 ASPETTETLRFAPGLTATVNGGLPVMIQERS 495

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O811172.3e-10041.83Cytochrome P450 94A1 OS=Vicia sativa OX=3908 GN=CYP94A1 PE=2 SV=2[more]
P981881.4e-9741.14Cytochrome P450 94A2 OS=Vicia sativa OX=3908 GN=CYP94A2 PE=2 SV=1[more]
Q9FMV71.4e-9742.80Cytochrome P450 94B1 OS=Arabidopsis thaliana OX=3702 GN=CYP94B1 PE=1 SV=1[more]
Q9SMP51.4e-9740.95Cytochrome P450 94B3 OS=Arabidopsis thaliana OX=3702 GN=CYP94B3 PE=1 SV=1[more]
Q9ZUX13.2e-9442.57Cytochrome P450 94C1 OS=Arabidopsis thaliana OX=3702 GN=CYP94C1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023528952.10.095.74cytochrome P450 94A1-like [Cucurbita pepo subsp. pepo] >XP_023528965.1 cytochrom... [more]
XP_023522613.10.095.54cytochrome P450 94A1-like [Cucurbita pepo subsp. pepo][more]
KAG7017629.10.091.89Cytochrome P450 94A1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6580873.10.091.28Cytochrome P450 94A1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022935257.10.090.87cytochrome P450 94A1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1F5250.090.87cytochrome P450 94A1-like OS=Cucurbita moschata OX=3662 GN=LOC111442194 PE=3 SV=... [more]
A0A6J1J8E10.089.59cytochrome P450 94A1-like OS=Cucurbita maxima OX=3661 GN=LOC111482283 PE=3 SV=1[more]
A0A5D3DPN21.39e-24869.64Cytochrome P450 94A1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3B7281.39e-24869.64cytochrome P450 94A1-like OS=Cucumis melo OX=3656 GN=LOC103486871 PE=3 SV=1[more]
A0A6J1EZ742.26e-24469.23cytochrome P450 94A1-like OS=Cucurbita moschata OX=3662 GN=LOC111440896 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G56630.13.1e-15655.13cytochrome P450, family 94, subfamily D, polypeptide 2 [more]
AT1G34540.14.6e-15254.71cytochrome P450, family 94, subfamily D, polypeptide 1 [more]
AT3G48520.11.0e-9840.95cytochrome P450, family 94, subfamily B, polypeptide 3 [more]
AT5G63450.11.0e-9842.80cytochrome P450, family 94, subfamily B, polypeptide 1 [more]
AT2G27690.12.3e-9542.57cytochrome P450, family 94, subfamily C, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 282..308
score: 21.58
coord: 330..348
score: 29.95
coord: 408..418
score: 44.21
coord: 262..279
score: 29.98
coord: 418..441
score: 20.04
IPR001128Cytochrome P450PRINTSPR00385P450coord: 418..429
score: 31.49
coord: 331..342
score: 44.75
coord: 409..418
score: 45.98
coord: 273..290
score: 36.0
IPR001128Cytochrome P450PFAMPF00067p450coord: 66..457
e-value: 1.3E-63
score: 215.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 10..472
e-value: 1.2E-94
score: 319.6
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 16..470
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 9..467
NoneNo IPR availablePANTHERPTHR24296:SF165CYTOCHROME P450 94B3-LIKEcoord: 9..467
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 411..420

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG03g04160.1Cp4.1LG03g04160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen