Cp4.1LG03g03850 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g03850
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	cytochrome P450, family 705, subfamily A, polypeptide 3
Location	Cp4.1LG03: 2081713 .. 2083481 (+)
RNA-Seq Expression	Cp4.1LG03g03850
Synteny	Cp4.1LG03g03850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTAGATTGCTCCCTACCTATCACCACAATGAACACCATGGCCACCATCTTTCATAACATCACCTTTCCCTCTCAATTTTGGTGCTTTTGGTTCATCACTGCCCTCCTCCTTCATTTCTTCCTCAAAAAACTTCTCCCAAACCCTAAATCCTCGATCCCCGGCGGTTACGCGCTGCCCCCGAGCCCACCGGCCCTCCCTCTCATCGGCCATCTCCACCTTCTCACCCCAGTTCTAGTCACCTCCTTCCAAACCCTAGCTCGTCGTTATGGCTCCCTCATCGAGCTTCGACTCGGCGCATCCAAATGCGTCGTCGTCTCTACCGCTACAGTTGCCAAAGAGGTCCTCAAAACTCACGAGCACAATTTCCTCTCCCGACCGGAGTTCGGTGCCTCCGAGCACTTCATATACCGAGGGTCGAGATTCGTCATGGCTCCCTACGGACCCTATTGGCGATTCATGAAGAAACTTACCATGACACGGCTTCTCTCACCGCCACAGCTCGCTATCGCCTCCGCAATCCGAAGGGACGAGGTGGGTAAACTAGTGGAGAGAATTGAAGCAAATTCAAGAGAAAAGAAACCATCGGATTTGAGATTGGAATTTACCACTTTGACAAATAATATCATATCAAGAATGCTTTTGAGCACAAGATGTTGTGATGGAATTGATGAAGCTCAAGAAGTTCAAGATTTGATGTGGAAGATCAATAGGCTTGCTGGGAAATTGAGTTTGGGAGACATTTTGGGACCTTTCAAAGTGCTTGATTTCTCTGGAAATGGCAAGAAATTTGTTAGAACCATGAAGAGATTTGATGGGTTGGTGGAGAGGATAATGAAGGAGCATGAAGCCGCCATCAATGGCGGCGGCGATCCCGACCGAAAACTAGACGTATTGGACATCTTGCTCGAGTGTCACAACGATCCAAATGCGGAGATGAACATAACAAGAAAAGATATCAAATCCTTCTTGCTCGTGAGTTCGTGATTTTAATTTTTGCATGTTTTTTTTTTAAGAATCACGATTCTCCATAATGATATGATATTGTTTACTTTTAGGATCTTTTCATGGCGGGCACTGACACGACAGCCACAGCGATATTATGGGCGATGGCTGAGCTTTTGAATCGTCCAAAATCAATGGAGGAGTTAAGAAAAGAGATAATTTCGGTGGTCGGAGAGGAGAAGCTAGTTCAAGAATCCGACCTCCCTAACCTCCCGTATCTCCGTGCGGTGGTGAACGAAACACTCCGGCTGCACCCATCAGCGCCGTTGATCATCAGAGAATGTTTAGATGACTGCATCATCAACGGTTCACTAGTCAAGGCGAAAACACGAGTCTTGGTCAACGCTTATGCTGTCATGAGAGACCCAGAATCTTGGTCGGAGCCCGACAGGTTCTTGCCGGAGAGGTTTCTAGAAGGGTCAAATGAGAACATTGGTAGCCACCGTATGGAGATGAAGGGGCAAAATTTCCGGTATATTCCGTTTGGGAGTGGGAAGAGAGGTTGCCCTGGTTCCTCCCTTGCCTTGCTAGTTTTCCCATGTGCAATGGCAGCCATGGTGCAGCGGTTTGATTGGAAGGTTGACGGTGACGGCGACGGCAACACCATCGATTTGACACCCGGGCCAGGTTTCGCTGCCGAGATGGCAACACCGCTCGTTCGTTATGCCGACCCCATTAATAAGAATACTTAATTATAAATTCTGTCGATATGCATCTTATCCGTGTATAAATATATCTCTTAAGTTTCTCTAAGTCAATTCGAATA

mRNA sequence

AGTAGATTGCTCCCTACCTATCACCACAATGAACACCATGGCCACCATCTTTCATAACATCACCTTTCCCTCTCAATTTTGGTGCTTTTGGTTCATCACTGCCCTCCTCCTTCATTTCTTCCTCAAAAAACTTCTCCCAAACCCTAAATCCTCGATCCCCGGCGGTTACGCGCTGCCCCCGAGCCCACCGGCCCTCCCTCTCATCGGCCATCTCCACCTTCTCACCCCAGTTCTAGTCACCTCCTTCCAAACCCTAGCTCGTCGTTATGGCTCCCTCATCGAGCTTCGACTCGGCGCATCCAAATGCGTCGTCGTCTCTACCGCTACAGTTGCCAAAGAGGTCCTCAAAACTCACGAGCACAATTTCCTCTCCCGACCGGAGTTCGGTGCCTCCGAGCACTTCATATACCGAGGGTCGAGATTCGTCATGGCTCCCTACGGACCCTATTGGCGATTCATGAAGAAACTTACCATGACACGGCTTCTCTCACCGCCACAGCTCGCTATCGCCTCCGCAATCCGAAGGGACGAGGTGGGTAAACTAGTGGAGAGAATTGAAGCAAATTCAAGAGAAAAGAAACCATCGGATTTGAGATTGGAATTTACCACTTTGACAAATAATATCATATCAAGAATGCTTTTGAGCACAAGATGTTGTGATGGAATTGATGAAGCTCAAGAAGTTCAAGATTTGATGTGGAAGATCAATAGGCTTGCTGGGAAATTGAGTTTGGGAGACATTTTGGGACCTTTCAAAGTGCTTGATTTCTCTGGAAATGGCAAGAAATTTGTTAGAACCATGAAGAGATTTGATGGGTTGGTGGAGAGGATAATGAAGGAGCATGAAGCCGCCATCAATGGCGGCGGCGATCCCGACCGAAAACTAGACGAGTTAAGAAAAGAGATAATTTCGGTGGTCGGAGAGGAGAAGCTAGTTCAAGAATCCGACCTCCCTAACCTCCCGTATCTCCGTGCGGTGGTGAACGAAACACTCCGGCTGCACCCATCAGCGCCTTTCTCTAAGTCAATTCGAATA

Coding sequence (CDS)

ATGAACACCATGGCCACCATCTTTCATAACATCACCTTTCCCTCTCAATTTTGGTGCTTTTGGTTCATCACTGCCCTCCTCCTTCATTTCTTCCTCAAAAAACTTCTCCCAAACCCTAAATCCTCGATCCCCGGCGGTTACGCGCTGCCCCCGAGCCCACCGGCCCTCCCTCTCATCGGCCATCTCCACCTTCTCACCCCAGTTCTAGTCACCTCCTTCCAAACCCTAGCTCGTCGTTATGGCTCCCTCATCGAGCTTCGACTCGGCGCATCCAAATGCGTCGTCGTCTCTACCGCTACAGTTGCCAAAGAGGTCCTCAAAACTCACGAGCACAATTTCCTCTCCCGACCGGAGTTCGGTGCCTCCGAGCACTTCATATACCGAGGGTCGAGATTCGTCATGGCTCCCTACGGACCCTATTGGCGATTCATGAAGAAACTTACCATGACACGGCTTCTCTCACCGCCACAGCTCGCTATCGCCTCCGCAATCCGAAGGGACGAGGTGGGTAAACTAGTGGAGAGAATTGAAGCAAATTCAAGAGAAAAGAAACCATCGGATTTGAGATTGGAATTTACCACTTTGACAAATAATATCATATCAAGAATGCTTTTGAGCACAAGATGTTGTGATGGAATTGATGAAGCTCAAGAAGTTCAAGATTTGATGTGGAAGATCAATAGGCTTGCTGGGAAATTGAGTTTGGGAGACATTTTGGGACCTTTCAAAGTGCTTGATTTCTCTGGAAATGGCAAGAAATTTGTTAGAACCATGAAGAGATTTGATGGGTTGGTGGAGAGGATAATGAAGGAGCATGAAGCCGCCATCAATGGCGGCGGCGATCCCGACCGAAAACTAGACGAGTTAAGAAAAGAGATAATTTCGGTGGTCGGAGAGGAGAAGCTAGTTCAAGAATCCGACCTCCCTAACCTCCCGTATCTCCGTGCGGTGGTGAACGAAACACTCCGGCTGCACCCATCAGCGCCTTTCTCTAAGTCAATTCGAATA

Protein sequence

MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLDELRKEIISVVGEEKLVQESDLPNLPYLRAVVNETLRLHPSAPFSKSIRI

Homology

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BLAST of Cp4.1LG03g03850 vs. ExPASy Swiss-Prot
Match: Q9XGT9 (Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 8.3e-52
Identity = 134/371 (36.12%), Postives = 188/371 (50.67%), Query Frame = 0


Query: 17  FWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLHLLTPVLVTSFQTL 76
           F  F+F T L L+    K   N +        LPPSPP+ P+IGHLH L P++  SF  L
Sbjct: 8   FLLFFFPTLLFLYCLPYKRNQNHR-------RLPPSPPSFPIIGHLHHLGPLIHQSFHAL 67

Query: 77  ARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGSRFVMAP 136
           + RYGSLI LRLG+  CVVVST  +AK+ LKT+E  F SR    A +H  Y G  F  AP
Sbjct: 68  STRYGSLIHLRLGSVPCVVVSTPDLAKDFLKTNELAFSSRKHSLAIDHITY-GVAFAFAP 127

Query: 137 YGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANSREKKPSDLRLEFTTLT 196
           YG YW+F+KKL    LL    L+    IR  E+ +L+  +   SR K+  +L  E   LT
Sbjct: 128 YGTYWKFIKKLFTVELLGTQNLSHFLPIRTHEIRELLRTLMVKSRAKERVNLTEELLKLT 187

Query: 197 NNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPFKVLDFSGNGKKFVR 256
           NN+IS+M++S RC     EA E ++L+ ++ ++ G+ ++ D +   K +D  G  K++  
Sbjct: 188 NNVISQMMMSIRCSGTNSEADEAKNLVREVTKIFGQFNVSDFIWFCKNIDLQGFKKRYEG 247

Query: 257 TMKRFDGLVERIMKEHE-----AAINGGGDPD---------------------------- 316
           T +R+D L+ERI+   E       I  G   D                            
Sbjct: 248 THRRYDALLERIIMGREENRRRGKIKDGEGKDFLDMLLDVLEDGKAEIKITRDHIKALIL 307

Query: 317 -------------------------RKLDELRKEIISVVGEEKLVQESDLPNLPYLRAVV 330
                                      L++ R+EI  V+G+E+LVQESD PNLPY++A++
Sbjct: 308 DFLTAGTDTTAIAIEWALVELINNPNALEKARQEIDQVIGDERLVQESDTPNLPYIQAII 367

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BLAST of Cp4.1LG03g03850 vs. ExPASy Swiss-Prot
Match: Q9XHC6 (Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 5.0e-49
Identity = 129/369 (34.96%), Postives = 183/369 (49.59%), Query Frame = 0


Query: 21  WFITALLLHFFLKKLLPNPKSSIPGGYALPPSPP-ALPLIGHLHLLTPVLVTSFQTLARR 80
           WFI+ +L+    KK         P    LPP PP ++PL+GH   L  +L  +   L+ R
Sbjct: 14  WFISTILIRSIFKK---------PQRLRLPPGPPISIPLLGHAPYLRSLLHQALYKLSLR 73

Query: 81  YGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGSRFVMAPYGP 140
           YG LI + +G+   VV S+A  AK++LKT E  F +RP   ASE   Y  + +   PYG 
Sbjct: 74  YGPLIHVMIGSKHVVVASSAETAKQILKTSEEAFCNRPLMIASESLTYGAADYFFIPYGT 133

Query: 141 YWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVER-IEANSREKKPSDLRLEFTTLTNN 200
           YWRF+KKL MT LLS   L     IR  EV   ++R +E +        +R E  T TNN
Sbjct: 134 YWRFLKKLCMTELLSGKTLEHFVRIRESEVEAFLKRMMEISGNGNYEVVMRKELITHTNN 193

Query: 201 IISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPFKVLDFSGNGKKFVRTM 260
           II+RM++  +     DE   ++ ++ ++  L G  +LGD++G  + LD  G GKK + T 
Sbjct: 194 IITRMIMGKKSNAENDEVARLRKVVREVGELLGAFNLGDVIGFMRPLDLQGFGKKNMETH 253

Query: 261 KRFDGLVERIMKEH-EAAINGGGDPDRKLD------------------------------ 320
            + D ++E++++EH EA      D DRK D                              
Sbjct: 254 HKVDAMMEKVLREHEEARAKEDADSDRKKDLFDILLNLIEADGADNKLTRESAKAFALDM 313

Query: 321 ---------------------------ELRKEIISVVGEEKLVQESDLPNLPYLRAVVNE 330
                                      + R+EI SVVG+E+LV+ESD+PNLPYL+AV+ E
Sbjct: 314 FIAGTNGPASVLEWSLAELVRNPHVFKKAREEIESVVGKERLVKESDIPNLPYLQAVLKE 373

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 6.2e-47
Identity = 119/344 (34.59%), Postives = 175/344 (50.87%), Query Frame = 0


Query: 49  LPPSPPALPLIGHLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKT 108
           LPPSP ALP+IGHLHL++P+    F  L+ R+G +++L LG+  CVV STA  AKE LKT
Sbjct: 29  LPPSPKALPIIGHLHLVSPIPHQDFYKLSTRHGPIMQLFLGSVPCVVASTAEAAKEFLKT 88

Query: 109 HEHNFLSRPEFGASEHFIYRGSR---FVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIR 168
           HE NF +RP    +   +   S+   F  AP+GPYW+FMKKL M+ LLS   +     +R
Sbjct: 89  HEINFSNRPGQNVAVKGLAYDSQDFLFAFAPFGPYWKFMKKLCMSELLSGRMMDQFLPVR 148

Query: 169 RDEVGKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWK 228
           + E  + + R+       +  D   E  TL+NNI+SRM LS +  +  ++A+E++ L+  
Sbjct: 149 QQETKRFISRVFRKGVAGEAVDFGDELMTLSNNIVSRMTLSQKTSENDNQAEEMKKLVSN 208

Query: 229 INRLAGKLSLGDILGPFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDP--- 288
           I  L GK ++ D +   K  D  G  +K   T  RFD +V+ I+K+ +       +    
Sbjct: 209 IAELMGKFNVSDFIWYLKPFDLQGFNRKIKETRDRFDVVVDGIIKQRQEERRKNKETGTA 268

Query: 289 -----------------------DRK---------------------------------- 330
                                  D+K                                  
Sbjct: 269 KQFKDMLDVLLDMHEDENAEIKLDKKNIKAFIMDIFVAGTDTSAVSIEWAMAELINNPDV 328

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 8.9e-46
Identity = 120/343 (34.99%), Postives = 170/343 (49.56%), Query Frame = 0


Query: 49  LPPSPPALPLIGHLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKT 108
           LPPSP  LP+IGHLHLL+P     F  L+ RYG +I L LG+  CVV STA  AKE LKT
Sbjct: 31  LPPSPMPLPIIGHLHLLSPTPHQDFHKLSLRYGPIIHLFLGSVPCVVASTAEAAKEFLKT 90

Query: 109 HEHNFLSRP-EFGASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRD 168
           HE  F +RP    A E   Y    F+ APYGPYW+FMKKL M+ LL    L     +R+ 
Sbjct: 91  HEPAFSNRPANTVAVETLTYGFQDFLFAPYGPYWKFMKKLCMSELLGGHMLDQFLPVRQX 150

Query: 169 EVGKLVERIEANSREKKPSDLRLEFTTLTNNIISRMLLS-TRCCDGIDEAQEVQDLMWKI 228
           E  K ++R+       +  D   EF TL+NNI+SRM++S T   +  +E +E++ L+   
Sbjct: 151 ETKKFIKRVLQKGISGEAVDFGGEFITLSNNIVSRMIVSQTSTTEDENEVEEMRKLVKDA 210

Query: 229 NRLAGKLSLGDILGPFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRK- 288
             L+GK ++ D +   K  D  G  K+  +    FD +++RI+K+ E       +   K 
Sbjct: 211 AELSGKFNISDFVSFLKRFDLQGFNKRLEKIRDCFDTVLDRIIKQREEERRNKNETVGKR 270

Query: 289 -----------------------------------------------------------L 330
                                                                      L
Sbjct: 271 EFKDMLDVLFDISEDESSEIKLNKENIKAFILDILIAGTDTSAVTMEWAMAELINNPGVL 330

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy Swiss-Prot
Match: E9KBR8 (Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.4e-43
Identity = 117/372 (31.45%), Postives = 187/372 (50.27%), Query Frame = 0


Query: 22  FITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLHLLTPVLVTSFQTLARRYG 81
           FI+A LL     +    PK+ +      PPSPPA+P+IGHLHLL P++  SF+ L+ RYG
Sbjct: 14  FISASLLKLLFVR-ENKPKAHLKN----PPSPPAIPIIGHLHLLKPLIHHSFRDLSLRYG 73

Query: 82  SLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGSRFVMAPYGPYW 141
            L+ LR+G+ K +V ST ++A+E LKT+E  + SR    A     Y  + F  APY  YW
Sbjct: 74  PLLSLRIGSVKFIVASTPSLAQEFLKTNELTYSSRKMNMAINMVTYHNATFAFAPYDTYW 133

Query: 142 RFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANSREKKPSDLRLEFTTLTNNIIS 201
           +FMKKL+ T LL    L     IR  EV  +++ +   S+ ++  +L     +L+NN+IS
Sbjct: 134 KFMKKLSTTELLGNKTLGHFLPIRTREVHDIIQFLFHKSKAQESVNLTEALLSLSNNVIS 193

Query: 202 RMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPFKVLDFSGNGKKFVRTMKRF 261
           +M+LS +      +A++ + L+ ++ ++ G+ ++ D LG  K LD  G  K+ +   KR+
Sbjct: 194 QMMLSIKSSGTDSQAEQARTLVREVTQIFGEFNVSDFLGFCKNLDLQGFRKRALDIHKRY 253

Query: 262 DGLVERIMKEHE-----AAINGGGDPD--------------------------------- 321
           D L+E+I+ + E     + ++G  D D                                 
Sbjct: 254 DALLEKIISDREELRRKSKVDGCEDGDDEKVKDFLDILLDVAEQKECEVQLTRNHVKSLI 313

Query: 322 --------------------------RKLDELRKEIISVVGEEKLVQESDLPNLPYLRAV 330
                                     + L + ++E+  V G  +LV E+D+PNLPY+ A+
Sbjct: 314 LDYFTAATDTTAISVEWTIAELFNNPKVLKKAQEEVDRVTGNTQLVCEADIPNLPYIHAI 373

[Close]

BLAST of Cp4.1LG03g03850 vs. NCBI nr
Match: XP_023528514.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 631 bits (1628), Expect = 1.70e-224
Identity = 329/386 (85.23%), Postives = 329/386 (85.23%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG
Sbjct: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS
Sbjct: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLD------------- 300
           PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLD             
Sbjct: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLDVLDILLECHNDPN 300

Query: 301 --------------------------------------------ELRKEIISVVGEEKLV 329
                                                       ELRKEIISVVGEEKLV
Sbjct: 301 AEMNITRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEKLV 360

[Close]

BLAST of Cp4.1LG03g03850 vs. NCBI nr
Match: XP_022982904.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima])

HSP 1 Score: 608 bits (1568), Expect = 2.44e-215
Identity = 321/388 (82.73%), Postives = 323/388 (83.25%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           MNTMATIFHNIT PSQFWCFWFITALLLHFFLKKLLPNPKSSIPGG+A PPSPPALPLIG
Sbjct: 1   MNTMATIFHNITSPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGHAPPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTA VAKEVLKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTAAVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIA+AIRRDEVGKLVERIEANS
Sbjct: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           R KKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG
Sbjct: 181 RGKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGG--DPDRKLD----------- 300
           P KVLDFSGNGKKFV TMKRFDGLVERIMKEHEAAINGGG  DPDRKLD           
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAINGGGHPDPDRKLDVLDILLECHND 300

Query: 301 ----------------------------------------------ELRKEIISVVGEEK 329
                                                         ELRKEIISVVGEEK
Sbjct: 301 PHAEMNISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEK 360

[Close]

BLAST of Cp4.1LG03g03850 vs. NCBI nr
Match: XP_022934356.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita moschata])

HSP 1 Score: 605 bits (1561), Expect = 2.64e-214
Identity = 317/386 (82.12%), Postives = 321/386 (83.16%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           MN M TIFHNIT  S FWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG
Sbjct: 1   MNAMVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASE+FIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIA+AIRRDEVGKLVERIEANS
Sbjct: 121 ASEYFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLD------------- 300
           P KVLDFSGNGKKFV TMKRFDGLVERIMKEHEAA+NGG DPDRKLD             
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAVNGGRDPDRKLDVLDILLECHNDPN 300

Query: 301 --------------------------------------------ELRKEIISVVGEEKLV 329
                                                       ELRKEIISVVGE+KLV
Sbjct: 301 AEMNISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEKKLV 360

[Close]

BLAST of Cp4.1LG03g03850 vs. NCBI nr
Match: KAG7017572.1 (3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605 bits (1561), Expect = 2.64e-214
Identity = 317/386 (82.12%), Postives = 321/386 (83.16%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           MN M TIFHNIT  S FWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG
Sbjct: 1   MNAMVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASE+FIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIA+AIRRDEVGKLVERIEANS
Sbjct: 121 ASEYFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLD------------- 300
           P KVLDFSGNGKKFV TMKRFDGLVERIMKEHEAA+NGG DPDRKLD             
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAVNGGRDPDRKLDVLDILLECHNDPN 300

Query: 301 --------------------------------------------ELRKEIISVVGEEKLV 329
                                                       ELRKEIISVVGE+KLV
Sbjct: 301 AEMNISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEKKLV 360

[Close]

BLAST of Cp4.1LG03g03850 vs. NCBI nr
Match: KAG6580822.1 (3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 604 bits (1558), Expect = 5.49e-214
Identity = 315/377 (83.55%), Postives = 319/377 (84.62%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           MN M TIFHNIT  S FWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG
Sbjct: 1   MNAMVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASE+FIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIA+ IRRDEVGKLVERIEANS
Sbjct: 121 ASEYFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAVIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           REKK SDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG
Sbjct: 181 REKKASDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLD------------- 300
           P KVLDFSGNGKKFV TMKRFDGLVERIMKEHEAA+NGG DPDRKLD             
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAVNGGRDPDRKLDVLDILLECHNDPN 300

Query: 301 -----------------------------------ELRKEIISVVGEEKLVQESDLPNLP 329
                                              ELRKEIISVVGE+KLVQESDLPNLP
Sbjct: 301 AEMNISRKDIKSFLLVTTAILWAMAELLNRPKSMEELRKEIISVVGEKKLVQESDLPNLP 360

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy TrEMBL
Match: A0A6J1J5T8 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111481610 PE=3 SV=1)

HSP 1 Score: 608 bits (1568), Expect = 1.18e-215
Identity = 321/388 (82.73%), Postives = 323/388 (83.25%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           MNTMATIFHNIT PSQFWCFWFITALLLHFFLKKLLPNPKSSIPGG+A PPSPPALPLIG
Sbjct: 1   MNTMATIFHNITSPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGHAPPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTA VAKEVLKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTAAVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIA+AIRRDEVGKLVERIEANS
Sbjct: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           R KKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG
Sbjct: 181 RGKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGG--DPDRKLD----------- 300
           P KVLDFSGNGKKFV TMKRFDGLVERIMKEHEAAINGGG  DPDRKLD           
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAINGGGHPDPDRKLDVLDILLECHND 300

Query: 301 ----------------------------------------------ELRKEIISVVGEEK 329
                                                         ELRKEIISVVGEEK
Sbjct: 301 PHAEMNISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEEK 360

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy TrEMBL
Match: A0A6J1F1K7 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111441548 PE=3 SV=1)

HSP 1 Score: 605 bits (1561), Expect = 1.28e-214
Identity = 317/386 (82.12%), Postives = 321/386 (83.16%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           MN M TIFHNIT  S FWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG
Sbjct: 1   MNAMVTIFHNITSLSPFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASE+FIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIA+AIRRDEVGKLVERIEANS
Sbjct: 121 ASEYFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIAAAIRRDEVGKLVERIEANS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG
Sbjct: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLD------------- 300
           P KVLDFSGNGKKFV TMKRFDGLVERIMKEHEAA+NGG DPDRKLD             
Sbjct: 241 PLKVLDFSGNGKKFVTTMKRFDGLVERIMKEHEAAVNGGRDPDRKLDVLDILLECHNDPN 300

Query: 301 --------------------------------------------ELRKEIISVVGEEKLV 329
                                                       ELRKEIISVVGE+KLV
Sbjct: 301 AEMNISRKDIKSFLLDLFMAGTDTTATAILWAMAELLNRPKSMEELRKEIISVVGEKKLV 360

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy TrEMBL
Match: A0A5D3DP65 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005640 PE=3 SV=1)

HSP 1 Score: 501 bits (1289), Expect = 2.25e-173
Identity = 263/385 (68.31%), Postives = 288/385 (74.81%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           M  M TIF N T PSQFW FWFITALLLH  LKK+L   K S P     PPSPPALPLIG
Sbjct: 1   MKAMTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPV+VTSFQTLARRYG LIE+RLGASKCV+VSTA VAKE+LKTHEHNFLSRPEFG
Sbjct: 61  HLHLLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASE+FIYRGSRFVMA YGPYWRFMKKLTMTRLLSPPQLA+++AIR DE+ KLVERIEA+S
Sbjct: 121 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           RE KPSD+RLEFTTLTNNIISRMLLSTRCC G DEA+E+++L W+IN LAGKLSLGDILG
Sbjct: 181 REGKPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRK--------------- 300
             KV DFSGNGKKFV T+K+FDGLVERIMKEHEAAI+ G +  +K               
Sbjct: 241 ALKVFDFSGNGKKFVETLKKFDGLVERIMKEHEAAIDSGDEERKKDLLDILLEIYNDPNA 300

Query: 301 -----------------------------------------LDELRKEIISVVGEEKLVQ 329
                                                    L +LRKEI SVVG +K VQ
Sbjct: 301 DMKITRTDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQ 360

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy TrEMBL
Match: A0A1S3B805 (LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103486788 PE=3 SV=1)

HSP 1 Score: 501 bits (1289), Expect = 3.49e-173
Identity = 265/385 (68.83%), Postives = 289/385 (75.06%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           M  M TIF N T PSQFW FWFITALLLH  LKK+L   K S P     PPSPPALPLIG
Sbjct: 15  MKAMTTIFDNTTSPSQFWSFWFITALLLHLLLKKILTKSKPSSPNTTKPPPSPPALPLIG 74

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPV+VTSFQTLARRYG LIE+RLGASKCV+VSTA VAKE+LKTHEHNFLSRPEFG
Sbjct: 75  HLHLLTPVIVTSFQTLARRYGPLIEIRLGASKCVIVSTAAVAKEILKTHEHNFLSRPEFG 134

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASE+FIYRGSRFVMA YGPYWRFMKKLTMTRLLSPPQLA+++AIR DE+ KLVERIEA+S
Sbjct: 135 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSDEIVKLVERIEASS 194

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           RE KPSD+RLEFTTLTNNIISRMLLSTRCC G DEA+E+++L W+IN LAGKLSLGDILG
Sbjct: 195 REGKPSDMRLEFTTLTNNIISRMLLSTRCCGGKDEAKEIKELAWRINMLAGKLSLGDILG 254

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRK--------------- 300
             KV DFSGNGKKFV T+K+FDGLVERIMKEHEAAI+ G D +RK               
Sbjct: 255 ALKVFDFSGNGKKFVETLKKFDGLVERIMKEHEAAIDSG-DEERKDLLDILLEIYNDPNA 314

Query: 301 -----------------------------------------LDELRKEIISVVGEEKLVQ 329
                                                    L +LRKEI SVVG +K VQ
Sbjct: 315 DMKITRTDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESLQKLRKEITSVVGNKKPVQ 374

[Close]

BLAST of Cp4.1LG03g03850 vs. ExPASy TrEMBL
Match: A0A0A0LBD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G810500 PE=3 SV=1)

HSP 1 Score: 481 bits (1237), Expect = 5.31e-160
Identity = 256/386 (66.32%), Postives = 282/386 (73.06%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           M  M TIF N T PSQFW F FIT LL H  L+KLL   + S P     PPSP ALP IG
Sbjct: 1   MKAMTTIFDNTTSPSQFWSFCFITILLFHLLLRKLLTKSRPSSPDTPKPPPSPSALPFIG 60

Query: 61  HLHLLTPVLVTSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFG 120
           HLHLLTPVLVTSFQTLARRYG LIE+RLGASKC+VVSTATVAKE+LKTHE NFLSRPEFG
Sbjct: 61  HLHLLTPVLVTSFQTLARRYGPLIEVRLGASKCIVVSTATVAKEILKTHEGNFLSRPEFG 120

Query: 121 ASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANS 180
           ASE+FIYRGSRFVMA YGPYWRFMKKLTMTRLLSPPQLA+++AIR +E+ KLVERIEA+S
Sbjct: 121 ASEYFIYRGSRFVMAQYGPYWRFMKKLTMTRLLSPPQLAVSTAIRSEEIAKLVERIEASS 180

Query: 181 REKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILG 240
           RE+KP DLRLEFTTLTNNIISRM+LSTRCC G DEA+E++DL W+IN LAGKLSLGDILG
Sbjct: 181 REEKPLDLRLEFTTLTNNIISRMVLSTRCCGGKDEAKEIKDLAWRINMLAGKLSLGDILG 240

Query: 241 PFKVLDFSGNGKKFVRTMKRFDGLVERIMKEHEAAINGGGDPDRKLD------------- 300
             KV DFSGNGKKFV+T+K+FD L+ERIMKEHEAAIN G D +RK D             
Sbjct: 241 ALKVFDFSGNGKKFVKTLKKFDELIERIMKEHEAAINDGEDEERKKDLLDILLEIYNDPN 300

Query: 301 --------------------------------------------ELRKEIISVVGEEKLV 329
                                                       +LR EI SVVG +K V
Sbjct: 301 ADFKLTRNDIKSFLLDLFMAGTDTTATAMLWAMGELLNCPESFHQLRNEITSVVGNKKPV 360

[Close]

BLAST of Cp4.1LG03g03850 vs. TAIR 10
Match: AT2G42250.1 (cytochrome P450, family 712, subfamily A, polypeptide 1 )

HSP 1 Score: 302.4 bits (773), Expect = 4.6e-82
Identity = 176/365 (48.22%), Postives = 229/365 (62.74%), Query Frame = 0


Query: 20  FWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLHLLTPVLVTSFQTLARR 79
           F F+   L  +FLK+     + S+     LP SPPALP IGHLHL+  VL  SFQ+LA +
Sbjct: 18  FPFMLYALFKWFLKE-----QGSL-AATKLPQSPPALPFIGHLHLIGKVLPVSFQSLAHK 77

Query: 80  YGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGSRFVMAPYGP 139
           YG L+E+RLGASKCVVVS+++VA+E+ K  E NF SRPEFG++E+F YRGSRFV+A YG 
Sbjct: 78  YGPLMEIRLGASKCVVVSSSSVAREIFKEQELNFSSRPEFGSAEYFKYRGSRFVLAQYGD 137

Query: 140 YWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANSREKKPSDLRLEFTTLTNNI 199
           YWRFMKKL MT+LL+ PQL   + IR +E  KLV+ +    RE  P DL  +F   TNN+
Sbjct: 138 YWRFMKKLCMTKLLAVPQLEKFADIREEEKLKLVDSVAKCCREGLPCDLSSQFIKYTNNV 197

Query: 200 ISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPFKVLDFSGNGKKFVRTMK 259
           I RM +STRC    +EA+E+++L+ K   LAGK+S+GD+LGP KV+DFSGNGKK V  M+
Sbjct: 198 ICRMAMSTRCSGTDNEAEEIRELVKKSLELAGKISVGDVLGPLKVMDFSGNGKKLVAVME 257

Query: 260 RFDGLVERIMKEHEA---------------AINGGGDPDRKL------------------ 319
           ++D LVERIMKE EA                +    DP  ++                  
Sbjct: 258 KYDLLVERIMKEREAKAKKKDGTRKDILDILLETYRDPTAEMKITRNDMKSFLLDVFMAG 317

Query: 320 ----------------------DELRKEIISVVGEEKLVQESDLPNLPYLRAVVNETLRL 330
                                 ++LR+EI +VVG ++LV+ESD+PNLPYLRAV+ ETLRL
Sbjct: 318 TDTSAAAMQWAMGQLINHPQAFNKLREEINNVVGSKRLVKESDVPNLPYLRAVLRETLRL 376

[Close]

BLAST of Cp4.1LG03g03850 vs. TAIR 10
Match: AT5G06905.1 (cytochrome P450, family 712, subfamily A, polypeptide 2 )

HSP 1 Score: 198.4 bits (503), Expect = 9.4e-51
Identity = 131/368 (35.60%), Postives = 188/368 (51.09%), Query Frame = 0


Query: 19  CFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLHLLTPVLVTSFQTLAR 78
           C +    L+  F L +L  +  SS  G   LPP P  LP++GH+HLL   L  S Q LA 
Sbjct: 6   CSYLFFTLVTIFLLHRLFSS--SSRRG---LPPGPRGLPVLGHMHLLRSSLPRSLQALAH 65

Query: 79  RYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGSRFVMAPYG 138
            YG L+ +R+G+ + +VVS +  AK +LKTH+ +F S+  FG  +  +Y+GS F  APYG
Sbjct: 66  TYGPLMTIRIGSLRVLVVSDSDTAKLILKTHDPDFASKFVFGPRQFNVYKGSEFFNAPYG 125

Query: 139 PYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANSREKKPSDLRLEFTTLTNN 198
            YWRFMKKL MT+L +  QL     IR +E   L+  +   SR  +  DL LEFT LT  
Sbjct: 126 SYWRFMKKLCMTKLFAGYQLDRFVDIREEETLALLSTLVERSRNGEACDLGLEFTALTTK 185

Query: 199 IISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDILGPFKVLDFSGNGKKFVRTM 258
           I+S+M++  RC    +  +E++ ++  I   A +    ++ GP + LD  GNGKK   ++
Sbjct: 186 ILSKMVMGKRCRQNSNIPKEIRKIVSDIMACATRFGFMELFGPLRDLDLFGNGKKLRSSI 245

Query: 259 KRFDGLVERIMKEHE----------------AAINGGGDPDRKL---------------- 318
            R+D LVE+I+KE+E                  ++   DP  +L                
Sbjct: 246 WRYDELVEKILKEYENDKSNEEEEKDKDIVDILLDTYNDPKAELRLTMNQIKFFILELFM 305

Query: 319 ------------------------DELRKEIISVVG-EEKLVQESDLPNLPYLRAVVNET 330
                                    ++R EI SVVG   +L++ESDL  LPYL+A + ET
Sbjct: 306 ASLDTTSAALQWTMTELINHPDIFAKIRDEIKSVVGTTNRLIKESDLQKLPYLQAAIKET 365

[Close]

BLAST of Cp4.1LG03g03850 vs. TAIR 10
Match: AT2G14100.1 (cytochrome P450, family 705, subfamily A, polypeptide 13 )

HSP 1 Score: 191.8 bits (486), Expect = 8.8e-49
Identity = 139/384 (36.20%), Postives = 190/384 (49.48%), Query Frame = 0


Query: 1   MNTMATIFHNITFPSQFWCFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIG 60
           M T+   F  I F  Q     F    +  FF KK    PK S   G  LPPSPP+LP+IG
Sbjct: 1   MATIVVDFQKIHFHPQILLSIFTVICIFVFFFKK----PKGS--RGCDLPPSPPSLPIIG 60

Query: 61  HLHLLTPVLV-TSFQTLARRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEF 120
           HLHLL   L   +FQ L+ +YG L+ LR+     V+VS+A+VA E+ KTH+ N  S    
Sbjct: 61  HLHLLLFDLPHKAFQKLSSKYGPLLCLRIFNVPIVLVSSASVAYEIFKTHDVNISSHGHP 120

Query: 121 GASEHFIYRGSRFVMAPYGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEAN 180
              E   +  S FVMAPYG YW+FMKKL +T+L  P  L  +   R DE+ +    + + 
Sbjct: 121 PIDECLFFGSSSFVMAPYGDYWKFMKKLMVTKLFGPQALEQSRGARADELERFHANLLSK 180

Query: 181 SREKKPSDLRLEFTTLTNNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDIL 240
             + +  ++  E   LTNN I +M++   C +   EA+ V+ L+ +   L  KL L  +L
Sbjct: 181 EMKSETVEIAKEAIKLTNNSICKMIMGRGCLEENGEAERVRGLVTETFALFKKLFLTQVL 240

Query: 241 GP-FKVLDFSGNGKKFVRTMKRFDGLVERIMKEHE------------------------- 300
              F++L  S   K+ +   ++FD L+ERI+ EHE                         
Sbjct: 241 RRLFEILRISPFKKETLDVSRKFDELLERIIVEHEEKTDYDHGMDLMDVLLAVYRDGKAE 300

Query: 301 -------------AAINGGGDPDRK---------------LDELRKEIISVVGEEKLVQE 330
                          I GG D   +               L+ LRKEI SVVG+ +L+QE
Sbjct: 301 YKITRDHLKSLFVELILGGTDTSAQTIEWTMAKIIKKPNILERLRKEIDSVVGKTRLIQE 360

[Close]

BLAST of Cp4.1LG03g03850 vs. TAIR 10
Match: AT3G20080.1 (cytochrome P450, family 705, subfamily A, polypeptide 15 )

HSP 1 Score: 186.0 bits (471), Expect = 4.8e-47
Identity = 130/368 (35.33%), Postives = 186/368 (50.54%), Query Frame = 0


Query: 19  CFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLHLLTPVLV-TSFQTLA 78
           CF+F+   L       L           Y LPPSPP LP+IGHLHLL  VLV  S QTL+
Sbjct: 12  CFFFVLLSLFSLLCYSLFFRKPKEPRLHYDLPPSPPTLPIIGHLHLLLSVLVHRSLQTLS 71

Query: 79  RRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGS-RFVMAP 138
            +YGS++ LR+ +    +VS+A++A E+ + H+ N  SR  F  ++  ++ GS  F  AP
Sbjct: 72  TKYGSILYLRVFSFPVALVSSASIAYEIFREHDVNISSR-GFPPTDDSLFAGSFSFTSAP 131

Query: 139 YGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANSREKKPSDLRLEFTTLT 198
           YG YW+FMKKL +T LL P  L  +   R DE+    E +   + +K+  D+ +E   L+
Sbjct: 132 YGDYWKFMKKLLVTNLLGPQALERSRGFRADELDLFYENLLDKAMKKESVDICVEALKLS 191

Query: 199 NNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDIL-GPFKVLDFSGNGKKFV 258
           NN I +M++   C +   EA+ V+ L  +++ L  K+ L ++L   FK L  S   K+ +
Sbjct: 192 NNSICKMIMGRSCSEENGEAERVRALATQLDGLTKKILLANMLRAGFKKLVVSLFRKEMM 251

Query: 259 RTMKRFDGLVERIMKEHE--------------AAINGGGDPDRK---------------- 318
               RFD L+ERI+ EHE              A +    D + +                
Sbjct: 252 DVSSRFDELLERILVEHEDKLDMHHQGTDLVDALLAACRDKNAEYKISRNHIKSFFADLL 311

Query: 319 ------------------------LDELRKEIISVVGEEKLVQESDLPNLPYLRAVVNET 330
                                   L+ LR EI SVVG+ +L+QE+DLPNLPYL+AVV E 
Sbjct: 312 FASTDTFVQTTQWTVAEIINNPNVLERLRGEIDSVVGKARLIQETDLPNLPYLQAVVKEG 371

[Close]

BLAST of Cp4.1LG03g03850 vs. TAIR 10
Match: AT3G20080.2 (cytochrome P450, family 705, subfamily A, polypeptide 15 )

HSP 1 Score: 186.0 bits (471), Expect = 4.8e-47
Identity = 130/368 (35.33%), Postives = 186/368 (50.54%), Query Frame = 0


Query: 19  CFWFITALLLHFFLKKLLPNPKSSIPGGYALPPSPPALPLIGHLHLLTPVLV-TSFQTLA 78
           CF+F+   L       L           Y LPPSPP LP+IGHLHLL  VLV  S QTL+
Sbjct: 12  CFFFVLLSLFSLLCYSLFFRKPKEPRLHYDLPPSPPTLPIIGHLHLLLSVLVHRSLQTLS 71

Query: 79  RRYGSLIELRLGASKCVVVSTATVAKEVLKTHEHNFLSRPEFGASEHFIYRGS-RFVMAP 138
            +YGS++ LR+ +    +VS+A++A E+ + H+ N  SR  F  ++  ++ GS  F  AP
Sbjct: 72  TKYGSILYLRVFSFPVALVSSASIAYEIFREHDVNISSR-GFPPTDDSLFAGSFSFTSAP 131

Query: 139 YGPYWRFMKKLTMTRLLSPPQLAIASAIRRDEVGKLVERIEANSREKKPSDLRLEFTTLT 198
           YG YW+FMKKL +T LL P  L  +   R DE+    E +   + +K+  D+ +E   L+
Sbjct: 132 YGDYWKFMKKLLVTNLLGPQALERSRGFRADELDLFYENLLDKAMKKESVDICVEALKLS 191

Query: 199 NNIISRMLLSTRCCDGIDEAQEVQDLMWKINRLAGKLSLGDIL-GPFKVLDFSGNGKKFV 258
           NN I +M++   C +   EA+ V+ L  +++ L  K+ L ++L   FK L  S   K+ +
Sbjct: 192 NNSICKMIMGRSCSEENGEAERVRALATQLDGLTKKILLANMLRAGFKKLVVSLFRKEMM 251

Query: 259 RTMKRFDGLVERIMKEHE--------------AAINGGGDPDRK---------------- 318
               RFD L+ERI+ EHE              A +    D + +                
Sbjct: 252 DVSSRFDELLERILVEHEDKLDMHHQGTDLVDALLAACRDKNAEYKISRNHIKSFFADLL 311

Query: 319 ------------------------LDELRKEIISVVGEEKLVQESDLPNLPYLRAVVNET 330
                                   L+ LR EI SVVG+ +L+QE+DLPNLPYL+AVV E 
Sbjct: 312 FASTDTFVQTTQWTVAEIINNPNVLERLRGEIDSVVGKARLIQETDLPNLPYLQAVVKEG 371

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g03850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGT9	8.3e-52	36.12	Cytochrome P450 93B2 OS=Gerbera hybrida OX=18101 GN=CYP93B2 PE=1 SV=1	[more]
Q9XHC6	5.0e-49	34.96	Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1	[more]
Q42798	6.2e-47	34.59	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1...	[more]
O81973	8.9e-46	34.99	Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1	[more]
E9KBR8	1.4e-43	31.45	Cytochrome P450 93B16 OS=Glycine max OX=3847 GN=CYP93B16 PE=1 SV=1	[more]

BLAST of Cp4.1LG03g03850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528514.1	1.70e-224	85.23	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo]	[more]
XP_022982904.1	2.44e-215	82.73	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima]	[more]
XP_022934356.1	2.64e-214	82.12	3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita moschata]	[more]
KAG7017572.1	2.64e-214	82.12	3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp...	[more]
KAG6580822.1	5.49e-214	83.55	3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp...	[more]

BLAST of Cp4.1LG03g03850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J5T8	1.18e-215	82.73	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F1K7	1.28e-214	82.12	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A5D3DP65	2.25e-173	68.31	3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3B805	3.49e-173	68.83	LOW QUALITY PROTEIN: 3,9-dihydroxypterocarpan 6A-monooxygenase OS=Cucumis melo O...	[more]
A0A0A0LBD2	5.31e-160	66.32	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G810500 PE=3 SV=1	[more]

BLAST of Cp4.1LG03g03850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42250.1	4.6e-82	48.22	cytochrome P450, family 712, subfamily A, polypeptide 1	[more]
AT5G06905.1	9.4e-51	35.60	cytochrome P450, family 712, subfamily A, polypeptide 2	[more]
AT2G14100.1	8.8e-49	36.20	cytochrome P450, family 705, subfamily A, polypeptide 13	[more]
AT3G20080.1	4.8e-47	35.33	cytochrome P450, family 705, subfamily A, polypeptide 15	[more]
AT3G20080.2	4.8e-47	35.33	cytochrome P450, family 705, subfamily A, polypeptide 15	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 315..333 score: 32.97 coord: 77..96 score: 32.38 coord: 101..122 score: 27.07
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 46..284 e-value: 4.7E-39 score: 136.2
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 285..335 e-value: 7.1E-14 score: 53.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 47..330
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 287..330 e-value: 1.6E-10 score: 40.4 coord: 50..277 e-value: 5.5E-24 score: 84.7
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 285..330
None	No IPR available	PANTHER	PTHR24298:SF204	CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1	coord: 8..287
None	No IPR available	PANTHER	PTHR24298:SF204	CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1	coord: 285..330
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 8..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g03850.1	Cp4.1LG03g03850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen