Cp4.1LG03g03580 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g03580 vs. ExPASy Swiss-Prot
Match: Q700D5 (Probable lysophospholipase BODYGUARD 4 OS=Arabidopsis thaliana OX=3702 GN=BDG4 PE=2 SV=1)

HSP 1 Score: 441.8 bits (1135), Expect = 9.2e-123
Identity = 214/419 (51.07%), Postives = 290/419 (69.21%), Query Frame = 0

Query: 31  RFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGE 90
           +F T++   ++F VF FLD LDAILCV+Y ++D+ LE  +T CYC +   +    D   E
Sbjct: 6   KFGTAIHAALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTAAAPQSQTTD---E 65

Query: 91  NEL-SETLCGRRNVFRRIALVGFSGSCKD--SEKMNNG----SAWNRWSDCGCSSCVDGM 150
           NEL SETL GRRN+FR +  +GF+   K   S K+        + NRWSDCGC SC    
Sbjct: 66  NELSSETLFGRRNIFRGMWFLGFAREFKSKLSRKLRKSKIHQESVNRWSDCGCKSCKSWT 125

Query: 151 ENRNQKLYVAVREPPRGRSG--KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLF 210
           +N +  L+V V++         +P ENVIF+HGF+ SS  WTE+VF ++   QK +YRL 
Sbjct: 126 KNEDGNLHVVVKDSTSREYSVQEPSENVIFIHGFMGSSHFWTETVFEHI---QKDDYRLL 185

Query: 211 AVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYS 270
           A+DLLGFG SPKPRD  YT+KDH++ IE SVI  + L+SFH+VAHSMGCLIALALAAK+S
Sbjct: 186 AIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKPYQLDSFHVVAHSMGCLIALALAAKHS 245

Query: 271 NSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICR 330
           N VKS+TLVAPPYFPS   G  +VL  +  KR+WPPL FG++VMSWYEH+GRC CF+IC+
Sbjct: 246 NIVKSVTLVAPPYFPSSVEG--SVLNRIARKRLWPPLAFGTAVMSWYEHIGRCVCFIICK 305

Query: 331 NHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSRAGV 390
           +H+IWEW+++    +R I +++ D+T+HTHHSAWHSMHNVICGG+K+ D +L+ L ++GV
Sbjct: 306 HHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMHNVICGGSKVADEHLETLIKSGV 365

Query: 391 KIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELTQDLEYIWAN 441
           KI++  GD D + P  CS N+K+      V+++   DH ++I GR  E  + LE IW +
Sbjct: 366 KIHLMQGDCDQIVPSHCSGNMKRTFPAVEVDIITGADHDSMISGRGEEFAEKLESIWCS 416

BLAST of Cp4.1LG03g03580 vs. ExPASy Swiss-Prot
Match: Q9FN79 (Probable lysophospholipase BODYGUARD 5 OS=Arabidopsis thaliana OX=3702 GN=BDG5 PE=3 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 6.6e-105
Identity = 195/424 (45.99%), Postives = 268/424 (63.21%), Query Frame = 0

Query: 41  NFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGR 100
           ++ VF   D LD  LC+++R++D+ +E K+ +C+C +  E+   A+ +    LSETL  R
Sbjct: 19  SWAVFFLFDLLDYFLCIVFRFLDEVMEEKSESCHCNNPREKTHFAEYE---FLSETLYRR 78

Query: 101 RNVFRRIALVGFSGSCKDSEK----------------MNNGS--AWNRWSDCGCSSCVDG 160
           RNVFR+   + F+    +  K                MN  S    NRWSDCGC +CV  
Sbjct: 79  RNVFRQAGFLRFANKLPEITKKIGIVTFLRKFLFPHTMNKVSHEVANRWSDCGCKTCVSW 138

Query: 161 MENRNQKLYVAVREP---PRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLSE-TQKLNY 220
           +     KL V V++P       S KP ENVIF+HGF++SSS WT +VF  L E T+  NY
Sbjct: 139 I--NTDKLNVIVKQPSISDLSISNKPVENVIFVHGFLASSSFWTNTVFKYLPETTEGTNY 198

Query: 221 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAA 280
           R FA+DLLGFG SPKPR   Y++K+H+E IE+SVI    L SFH+VAHSMGC+I +ALAA
Sbjct: 199 RFFAIDLLGFGDSPKPRASQYSLKEHVEMIEKSVILPNNLTSFHVVAHSMGCIIGIALAA 258

Query: 281 KYSNSVKSITLVAPPYF-PSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACF 340
           K+S+SVKS+ LVAPPYF  SK G +   L+ +  K++WPP  F +++M WYEH+GR  C 
Sbjct: 259 KFSDSVKSVALVAPPYFADSKGGASCAALDVVAKKKLWPPASFFTAMMCWYEHIGRGVCL 318

Query: 341 VICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELS 400
           V CR+HR WE I++ +  RR +   ++D TKHTH S WHSMHNVICGGAK TD +L+ L 
Sbjct: 319 VFCRHHRTWERIIKIVTWRRKLPTAIMDFTKHTHQSGWHSMHNVICGGAKFTDKHLETLI 378

Query: 401 RAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELTQDLEYI 442
           ++GVKI +  GD+DVV P++C  N+K K     V ++   DH T+I+ R      +L  +
Sbjct: 379 KSGVKINVMQGDKDVVVPIDCLSNMKGKFPAVEVEVIAGTDHSTVIMSRREVFAANLVSL 437

BLAST of Cp4.1LG03g03580 vs. ExPASy Swiss-Prot
Match: Q8LFX7 (Probable lysophospholipase BODYGUARD 1 OS=Arabidopsis thaliana OX=3702 GN=BDG1 PE=2 SV=1)

HSP 1 Score: 347.1 bits (889), Expect = 3.1e-94
Identity = 176/458 (38.43%), Postives = 265/458 (57.86%), Query Frame = 0

Query: 32  FRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYC------------GSRG 91
           F  SL   V   VF  LD +D +LC  Y+ +D F E +   CYC            G+RG
Sbjct: 3   FSRSLNRTVGVFVFFILDIVDFLLCFTYKTLDFFFESEWKPCYCCPPPEAKPISAGGNRG 62

Query: 92  EERGNADGDGE--------------NELSETLCGRRNVFRRIALV------GFSGSCKDS 151
            +   ++  G+              +E+S+TL  R ++  ++  +           C +S
Sbjct: 63  GKMIVSERSGDYSKVVSLTRTKIYLDEISDTLYSRPSLLTKLTKLVKCFKKDVVKCCDES 122

Query: 152 EKMNNGSAWN----------------RWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSG 211
           +K +  +                   RWSDC C+ C   + + NQ L+V V++P      
Sbjct: 123 KKRSPSTKKTLLTVNSTVVEKLQRTPRWSDCHCTFCTSWLSSSNQSLFVNVQQP---TDN 182

Query: 212 KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKD 271
           K +ENV+F+HGF+SSS+ WTE++FPN S++ K NYR  AVDLLG+G+SPKP D  YT+K+
Sbjct: 183 KAQENVVFIHGFLSSSTFWTETLFPNFSDSAKSNYRFLAVDLLGYGKSPKPNDSLYTLKE 242

Query: 272 HLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--G 331
           HLE IE SVI QF L +FHLVAHS+GC++ALALA K+  ++KS+TL+APPY+    G  G
Sbjct: 243 HLEMIERSVISQFRLKTFHLVAHSLGCILALALAVKHPGAIKSLTLLAPPYYSVPKGVQG 302

Query: 332 AMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDF 391
              V+  L  K VWPP+ FG+SV SWYEH+ R    V+C+NH + E++ R +   R   +
Sbjct: 303 TQYVMRRLAPKEVWPPMAFGASVASWYEHISRTVSLVLCKNHHLLEFLTRLLTRNRMRTY 362

Query: 392 RVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPVECSY 439
            +     HTH+++WH++HN+I G     + YLD +      ++ ++HG +D + PVECSY
Sbjct: 363 LIEGFLCHTHNASWHTLHNIIFGSGSKVEAYLDHVRDNVDCEVAVFHGGRDELIPVECSY 422

BLAST of Cp4.1LG03g03580 vs. ExPASy Swiss-Prot
Match: Q9FJ24 (Probable lysophospholipase BODYGUARD 2 OS=Arabidopsis thaliana OX=3702 GN=BDG2 PE=2 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 1.2e-90
Identity = 177/460 (38.48%), Postives = 266/460 (57.83%), Query Frame = 0

Query: 40  VNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNAD--------GDGE- 99
           V F VF  LD  D +LC  Y+ +D FLE +   CYC S  E +   +        G  + 
Sbjct: 11  VGFFVFALLDIADFLLCYTYKTLDYFLESERKPCYCSSPPEAKAKTEKIIVSERGGYSKV 70

Query: 100 ----------NELSETLCGR--------RNVFRRIALVGFSG---------SC---KDSE 159
                     +E+S+TL  R          + R +    + G         SC   +  +
Sbjct: 71  VSLTRSKIHFDEISDTLYSRGPSLLTRLSKLVRSVKCFNYKGLIMRGNVVESCDHHESKK 130

Query: 160 KMNNG---------------SAWNRWSDCGCSSCVDGMENRNQ-KLYVAVREPPRGRSGK 219
           K++ G               S   RWSDC CS C   + + N+  L+V V++P   +  K
Sbjct: 131 KISKGKKRLMTLNSTVIEKSSTAPRWSDCHCSFCTSWLTSTNRDSLFVKVQQPKDNK--K 190

Query: 220 PKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDH 279
            ++NV+F+HGFVSSS+ WTE++FPN S++ K NYR  AVDLLG+GRSPKP D  YT+++H
Sbjct: 191 ARDNVVFIHGFVSSSAFWTETLFPNFSDSAKSNYRFIAVDLLGYGRSPKPNDSLYTLREH 250

Query: 280 LEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--GA 339
           LE IE+SVI +F L +FH+VAHS+GC++ALALA K+  ++KS+TL+APPY+    G   A
Sbjct: 251 LEMIEKSVISKFKLKTFHIVAHSLGCILALALAVKHPGAIKSLTLLAPPYYKVPKGVQPA 310

Query: 340 MTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFR 399
             V+  +  K VWPP+ FG+SV+SWYEH+GR    V+ +NH++ E++ R +   R   + 
Sbjct: 311 QYVMREVARKEVWPPMQFGASVLSWYEHLGRTIGLVLIKNHQLIEFVTRLLTLNRMRTYL 370

Query: 400 VIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPVECSYN 442
           +     HTH+ ++H++HN+I G     D YLD +       + I+HG +D + PVECSY+
Sbjct: 371 IEGFLCHTHNGSFHTLHNIIFGSGAKLDSYLDHVRDHVDCDVAIFHGGKDELIPVECSYS 430

BLAST of Cp4.1LG03g03580 vs. ExPASy Swiss-Prot
Match: O22977 (Probable lysophospholipase BODYGUARD 3 OS=Arabidopsis thaliana OX=3702 GN=BDG3 PE=2 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 4.4e-85
Identity = 172/485 (35.46%), Postives = 269/485 (55.46%), Query Frame = 0

Query: 34  TSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGE----ERGN---AD 93
           T L + V+F VF  LD +D+ LC++Y+  D   E +   CYC S  E     RG    + 
Sbjct: 15  TWLNEAVSFVVFCILDIVDSFLCLLYKAADYLFEAEWKPCYCLSDKEPITTTRGKILLSH 74

Query: 94  GDGEN--------------------ELSETLCGRRNVFRRIALV---------------- 153
            +GE+                    ++SETL  R ++   I+ +                
Sbjct: 75  NNGESKILTLSPLQELGGRSKIELEDISETLYTRPSLISDISTISVNELNKRFVKVTRSE 134

Query: 154 ----GFSGSCKDSEK------------------------MNNGSAWNRWSDCGCSSCVD- 213
               G +   K+  +                         N     +RWSDC C  C   
Sbjct: 135 SECSGHNEKTKNKRRRSLTKSSLTVNFTVVEMLRGKIRPQNLNHDISRWSDCDCGFCTSW 194

Query: 214 -GMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRL 273
               ++N  L+V  + P        KE+V+F+HGF+SSS+ WTE+VFP+LS +    +RL
Sbjct: 195 ASTSDKNHSLFVKTQIP---NGVTAKEDVLFIHGFISSSAFWTETVFPSLSASSS-THRL 254

Query: 274 FAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKY 333
           FAVDLLGFG+SPKP D  YT+++H+E IE+SV+H++ + SFH+VAHS+GC++AL+LAA++
Sbjct: 255 FAVDLLGFGKSPKPADSLYTLREHVEMIEKSVLHKYNVKSFHIVAHSLGCILALSLAARH 314

Query: 334 SNSVKSITLVAPPYFPSKDG---GAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACF 393
              +KS+TL+APPY+P   G       V++ +  ++VWPP+  G+S+  WYEH+ R  C 
Sbjct: 315 GGLIKSLTLLAPPYYPVPKGEKKPRQYVMKKVAPRKVWPPIALGASMACWYEHISRTICL 374

Query: 394 VICRNHRIWEWI---LRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLD 439
           +IC++HR+W++I   L R N  R ++F +     HTH++AWH++HN+ICG     D YLD
Sbjct: 375 LICKHHRVWQFIAGVLTRNN--RTVNFLIEGFMCHTHNAAWHTLHNIICGTGSKLDTYLD 434

BLAST of Cp4.1LG03g03580 vs. NCBI nr
Match: XP_023526531.1 (probable lysophospholipase BODYGUARD 4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 929 bits (2401), Expect = 0.0
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60
           MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR
Sbjct: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120

Query: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180
           KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS
Sbjct: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420

Query: 421 TIILGRERELTQDLEYIWANTVEIERS 447
           TIILGRERELTQDLEYIWANTVEIERS
Sbjct: 421 TIILGRERELTQDLEYIWANTVEIERS 447

BLAST of Cp4.1LG03g03580 vs. NCBI nr
Match: KAG7017593.1 (putative lysophospholipase BODYGUARD 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 891 bits (2302), Expect = 0.0
Identity = 428/447 (95.75%), Postives = 437/447 (97.76%), Query Frame = 0

Query: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60
           MCFFTAVGFS   + FL MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDF+DAILCVIYR
Sbjct: 1   MCFFTAVGFSMSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFIDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETL GRR+VFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180
           KMN+GSAWNRWSDCGCSSCVDG ENRNQKLYVAVREPPRGRS KPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAVREPPRGRSEKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNI+FRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN V+N DHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQDLEYIWANTVEIERS 447
           TIILGRERELTQ+LEYIWANTV+IERS
Sbjct: 421 TIILGRERELTQELEYIWANTVDIERS 447

BLAST of Cp4.1LG03g03580 vs. NCBI nr
Match: XP_022934164.1 (probable lysophospholipase BODYGUARD 4 isoform X1 [Cucurbita moschata])

HSP 1 Score: 885 bits (2288), Expect = 0.0
Identity = 425/442 (96.15%), Postives = 431/442 (97.51%), Query Frame = 0

Query: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60
           MCFFTAVGFS   + FL MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETL GRR+VFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180
           KMN+GSAWNRWSDCGCSSCVDG ENRNQKLYVA REPPRGRSGKPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN V+N DHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQDLEYIWANTV 442
           TIILGRERELTQ+LEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 442

BLAST of Cp4.1LG03g03580 vs. NCBI nr
Match: XP_022934165.1 (probable lysophospholipase BODYGUARD 4 isoform X2 [Cucurbita moschata])

HSP 1 Score: 869 bits (2245), Expect = 0.0
Identity = 420/442 (95.02%), Postives = 426/442 (96.38%), Query Frame = 0

Query: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60
           MCFFTAVGFS   + FL MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETL GRR+VFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180
           KMN+GSAWNRWSDCGCSSCVDG ENRNQKLYVA REPPRGRSGKPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRR     VIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRR-----VIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN V+N DHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQDLEYIWANTV 442
           TIILGRERELTQ+LEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 437

BLAST of Cp4.1LG03g03580 vs. NCBI nr
Match: KAG6580837.1 (putative lysophospholipase BODYGUARD 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 867 bits (2241), Expect = 0.0
Identity = 415/429 (96.74%), Postives = 423/429 (98.60%), Query Frame = 0

Query: 19  MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSR 78
           MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSR
Sbjct: 1   MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSR 60

Query: 79  GEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSEKMNNGSAWNRWSDCGCSS 138
           GEERGNADGDGENELSETL GRR+VFRRIALVGFSGSCKDSEKMN+GSAWNRWSDCGCSS
Sbjct: 61  GEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSEKMNSGSAWNRWSDCGCSS 120

Query: 139 CVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNY 198
           CVDG ENRNQKLYVA REPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNY
Sbjct: 121 CVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNY 180

Query: 199 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAA 258
           RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALA+AA
Sbjct: 181 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALAIAA 240

Query: 259 KYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFV 318
           KYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFV
Sbjct: 241 KYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFV 300

Query: 319 ICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSR 378
           ICRNHRIWEWILRRINPRRNI+FRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSR
Sbjct: 301 ICRNHRIWEWILRRINPRRNINFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSR 360

Query: 379 AGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELTQDLEYIW 438
           AGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN V+N DHQTIILGRERELTQ+L YIW
Sbjct: 361 AGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQTIILGRERELTQELGYIW 420

Query: 439 ANTVEIERS 447
           ANTV+IERS
Sbjct: 421 ANTVDIERS 429

BLAST of Cp4.1LG03g03580 vs. ExPASy TrEMBL
Match: A0A6J1F1T1 (probable lysophospholipase BODYGUARD 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441414 PE=4 SV=1)

HSP 1 Score: 885 bits (2288), Expect = 0.0
Identity = 425/442 (96.15%), Postives = 431/442 (97.51%), Query Frame = 0

Query: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60
           MCFFTAVGFS   + FL MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETL GRR+VFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180
           KMN+GSAWNRWSDCGCSSCVDG ENRNQKLYVA REPPRGRSGKPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN V+N DHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQDLEYIWANTV 442
           TIILGRERELTQ+LEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 442

BLAST of Cp4.1LG03g03580 vs. ExPASy TrEMBL
Match: A0A6J1F6W3 (probable lysophospholipase BODYGUARD 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441414 PE=4 SV=1)

HSP 1 Score: 869 bits (2245), Expect = 0.0
Identity = 420/442 (95.02%), Postives = 426/442 (96.38%), Query Frame = 0

Query: 1   MCFFTAVGFSDERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60
           MCFFTAVGFS   + FL MDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR
Sbjct: 1   MCFFTAVGFSVSELTFLLMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYR 60

Query: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSE 120
           YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETL GRR+VFRRIALVGFSGSCKDSE
Sbjct: 61  YIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLYGRRSVFRRIALVGFSGSCKDSE 120

Query: 121 KMNNGSAWNRWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSS 180
           KMN+GSAWNRWSDCGCSSCVDG ENRNQKLYVA REPPRGRSGKPKENVIFLHGFVSSSS
Sbjct: 121 KMNSGSAWNRWSDCGCSSCVDGKENRNQKLYVAAREPPRGRSGKPKENVIFLHGFVSSSS 180

Query: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240
           LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS
Sbjct: 181 LWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNS 240

Query: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLF 300
           FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAK VWPPLLF
Sbjct: 241 FHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKSVWPPLLF 300

Query: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHN 360
           GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRR     VIDLTKHTHHSAWHSMHN
Sbjct: 301 GSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRR-----VIDLTKHTHHSAWHSMHN 360

Query: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQ 420
           VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAP+ECSYNLKKKAVDATVN V+N DHQ
Sbjct: 361 VICGGAKLTDGYLDELSRAGVKIYIYHGDQDVVAPIECSYNLKKKAVDATVNTVKNADHQ 420

Query: 421 TIILGRERELTQDLEYIWANTV 442
           TIILGRERELTQ+LEYIWANTV
Sbjct: 421 TIILGRERELTQELEYIWANTV 437

BLAST of Cp4.1LG03g03580 vs. ExPASy TrEMBL
Match: A0A6J1J489 (probable lysophospholipase BODYGUARD 4 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111481627 PE=4 SV=1)

HSP 1 Score: 856 bits (2211), Expect = 2.00e-312
Identity = 413/436 (94.72%), Postives = 420/436 (96.33%), Query Frame = 0

Query: 12  ERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT 71
             + FLFMDSSI S KWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT
Sbjct: 44  SELTFLFMDSSILSGKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT 103

Query: 72  ACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSEKMNNGSAWNRW 131
           ACYCGSRGEERGNADGDGENELSETL GRRNVFRRIALVGFSGSCKDSEKMN+GSAWNRW
Sbjct: 104 ACYCGSRGEERGNADGDGENELSETLYGRRNVFRRIALVGFSGSCKDSEKMNSGSAWNRW 163

Query: 132 SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS 191
           SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS
Sbjct: 164 SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS 223

Query: 192 ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCL 251
           ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIE SVIHQFGLNSFHLVAHSMGCL
Sbjct: 224 ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIE-SVIHQFGLNSFHLVAHSMGCL 283

Query: 252 IALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHV 311
           IALALAAKYSNSVK+ITLVAPPYFPSK G AM VLENL AKR+WPPLLFGSSVMSWYEHV
Sbjct: 284 IALALAAKYSNSVKTITLVAPPYFPSKHGVAMAVLENLAAKRIWPPLLFGSSVMSWYEHV 343

Query: 312 GRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDG 371
           GRCACF ICRNHRIWEWILRRINPR NIDFRVIDLTKHTHHSAWHSMHNVICGGAKL DG
Sbjct: 344 GRCACFFICRNHRIWEWILRRINPRSNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLMDG 403

Query: 372 YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELT 431
           YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMV+N DHQTIILGRERELT
Sbjct: 404 YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVKNADHQTIILGRERELT 463

Query: 432 QDLEYIWANTVEIERS 447
           QDLEYIWANT +IER+
Sbjct: 464 QDLEYIWANTADIERA 478

BLAST of Cp4.1LG03g03580 vs. ExPASy TrEMBL
Match: A0A6J1J6B6 (probable lysophospholipase BODYGUARD 4 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481627 PE=4 SV=1)

HSP 1 Score: 856 bits (2211), Expect = 2.92e-312
Identity = 413/436 (94.72%), Postives = 420/436 (96.33%), Query Frame = 0

Query: 12  ERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT 71
             + FLFMDSSI S KWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT
Sbjct: 44  SELTFLFMDSSILSGKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT 103

Query: 72  ACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSEKMNNGSAWNRW 131
           ACYCGSRGEERGNADGDGENELSETL GRRNVFRRIALVGFSGSCKDSEKMN+GSAWNRW
Sbjct: 104 ACYCGSRGEERGNADGDGENELSETLYGRRNVFRRIALVGFSGSCKDSEKMNSGSAWNRW 163

Query: 132 SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS 191
           SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS
Sbjct: 164 SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS 223

Query: 192 ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCL 251
           ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIE SVIHQFGLNSFHLVAHSMGCL
Sbjct: 224 ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIE-SVIHQFGLNSFHLVAHSMGCL 283

Query: 252 IALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHV 311
           IALALAAKYSNSVK+ITLVAPPYFPSK G AM VLENL AKR+WPPLLFGSSVMSWYEHV
Sbjct: 284 IALALAAKYSNSVKTITLVAPPYFPSKHGVAMAVLENLAAKRIWPPLLFGSSVMSWYEHV 343

Query: 312 GRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDG 371
           GRCACF ICRNHRIWEWILRRINPR NIDFRVIDLTKHTHHSAWHSMHNVICGGAKL DG
Sbjct: 344 GRCACFFICRNHRIWEWILRRINPRSNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLMDG 403

Query: 372 YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELT 431
           YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMV+N DHQTIILGRERELT
Sbjct: 404 YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVKNADHQTIILGRERELT 463

Query: 432 QDLEYIWANTVEIERS 447
           QDLEYIWANT +IER+
Sbjct: 464 QDLEYIWANTADIERA 478

BLAST of Cp4.1LG03g03580 vs. ExPASy TrEMBL
Match: A0A6J1J5V9 (probable lysophospholipase BODYGUARD 4 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481627 PE=4 SV=1)

HSP 1 Score: 842 bits (2174), Expect = 1.05e-306
Identity = 409/436 (93.81%), Postives = 416/436 (95.41%), Query Frame = 0

Query: 12  ERVDFLFMDSSISSRKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT 71
             + FLFMDSSI S KWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT
Sbjct: 44  SELTFLFMDSSILSGKWLIRFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTT 103

Query: 72  ACYCGSRGEERGNADGDGENELSETLCGRRNVFRRIALVGFSGSCKDSEKMNNGSAWNRW 131
           ACYCGSRGEERGNADGDGENELSETL GRRNVFRRIALVGFSGSCKDSEKMN+GSAWNRW
Sbjct: 104 ACYCGSRGEERGNADGDGENELSETLYGRRNVFRRIALVGFSGSCKDSEKMNSGSAWNRW 163

Query: 132 SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS 191
           SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS
Sbjct: 164 SDCGCSSCVDGMENRNQKLYVAVREPPRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLS 223

Query: 192 ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCL 251
           ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIE SVIHQFGLNSFHLVAHSMGCL
Sbjct: 224 ETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDHLEKIE-SVIHQFGLNSFHLVAHSMGCL 283

Query: 252 IALALAAKYSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHV 311
           IALALAAKYSNSVK+ITLVAPPYFPSK G AM VLENL AKR+WPPLLFGSSVMSWYEHV
Sbjct: 284 IALALAAKYSNSVKTITLVAPPYFPSKHGVAMAVLENLAAKRIWPPLLFGSSVMSWYEHV 343

Query: 312 GRCACFVICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDG 371
           GRCACF ICRNHRIWEWILRRINPRR     VIDLTKHTHHSAWHSMHNVICGGAKL DG
Sbjct: 344 GRCACFFICRNHRIWEWILRRINPRR-----VIDLTKHTHHSAWHSMHNVICGGAKLMDG 403

Query: 372 YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELT 431
           YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMV+N DHQTIILGRERELT
Sbjct: 404 YLDELSRAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVKNADHQTIILGRERELT 463

Query: 432 QDLEYIWANTVEIERS 447
           QDLEYIWANT +IER+
Sbjct: 464 QDLEYIWANTADIERA 473

BLAST of Cp4.1LG03g03580 vs. TAIR 10
Match: AT5G17780.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 441.8 bits (1135), Expect = 6.5e-124
Identity = 214/419 (51.07%), Postives = 290/419 (69.21%), Query Frame = 0

Query: 31  RFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGE 90
           +F T++   ++F VF FLD LDAILCV+Y ++D+ LE  +T CYC +   +    D   E
Sbjct: 6   KFGTAIHAALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTAAAPQSQTTD---E 65

Query: 91  NEL-SETLCGRRNVFRRIALVGFSGSCKD--SEKMNNG----SAWNRWSDCGCSSCVDGM 150
           NEL SETL GRRN+FR +  +GF+   K   S K+        + NRWSDCGC SC    
Sbjct: 66  NELSSETLFGRRNIFRGMWFLGFAREFKSKLSRKLRKSKIHQESVNRWSDCGCKSCKSWT 125

Query: 151 ENRNQKLYVAVREPPRGRSG--KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLF 210
           +N +  L+V V++         +P ENVIF+HGF+ SS  WTE+VF ++   QK +YRL 
Sbjct: 126 KNEDGNLHVVVKDSTSREYSVQEPSENVIFIHGFMGSSHFWTETVFEHI---QKDDYRLL 185

Query: 211 AVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYS 270
           A+DLLGFG SPKPRD  YT+KDH++ IE SVI  + L+SFH+VAHSMGCLIALALAAK+S
Sbjct: 186 AIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKPYQLDSFHVVAHSMGCLIALALAAKHS 245

Query: 271 NSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICR 330
           N VKS+TLVAPPYFPS   G  +VL  +  KR+WPPL FG++VMSWYEH+GRC CF+IC+
Sbjct: 246 NIVKSVTLVAPPYFPSSVEG--SVLNRIARKRLWPPLAFGTAVMSWYEHIGRCVCFIICK 305

Query: 331 NHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSRAGV 390
           +H+IWEW+++    +R I +++ D+T+HTHHSAWHSMHNVICGG+K+ D +L+ L ++GV
Sbjct: 306 HHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMHNVICGGSKVADEHLETLIKSGV 365

Query: 391 KIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELTQDLEYIWAN 441
           KI++  GD D + P  CS N+K+      V+++   DH ++I GR  E  + LE IW +
Sbjct: 366 KIHLMQGDCDQIVPSHCSGNMKRTFPAVEVDIITGADHDSMISGRGEEFAEKLESIWCS 416

BLAST of Cp4.1LG03g03580 vs. TAIR 10
Match: AT5G17780.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 441.4 bits (1134), Expect = 8.5e-124
Identity = 214/421 (50.83%), Postives = 291/421 (69.12%), Query Frame = 0

Query: 31  RFRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGE 90
           +F T++   ++F VF FLD LDAILCV+Y ++D+ LE  +T CYC +   +    D   E
Sbjct: 6   KFGTAIHAALSFIVFFFLDLLDAILCVVYEFVDEILEENSTGCYCTAAAPQSQTTD---E 65

Query: 91  NEL-SETLCGRRNVFRRIALVGFSGSCKD--SEKMNNG----SAWNRWSDCGCSSCVDGM 150
           NEL SETL GRRN+FR +  +GF+   K   S K+        + NRWSDCGC SC    
Sbjct: 66  NELSSETLFGRRNIFRGMWFLGFAREFKSKLSRKLRKSKIHQESVNRWSDCGCKSCKSWT 125

Query: 151 ENRNQKLYVAVREPPRGRS----GKPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYR 210
           +N +  L+V V++     +     +P ENVIF+HGF+ SS  WTE+VF ++   QK +YR
Sbjct: 126 KNEDGNLHVVVKDSTSRETEYSVQEPSENVIFIHGFMGSSHFWTETVFEHI---QKDDYR 185

Query: 211 LFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAK 270
           L A+DLLGFG SPKPRD  YT+KDH++ IE SVI  + L+SFH+VAHSMGCLIALALAAK
Sbjct: 186 LLAIDLLGFGESPKPRDSLYTLKDHVDTIERSVIKPYQLDSFHVVAHSMGCLIALALAAK 245

Query: 271 YSNSVKSITLVAPPYFPSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVI 330
           +SN VKS+TLVAPPYFPS   G  +VL  +  KR+WPPL FG++VMSWYEH+GRC CF+I
Sbjct: 246 HSNIVKSVTLVAPPYFPSSVEG--SVLNRIARKRLWPPLAFGTAVMSWYEHIGRCVCFII 305

Query: 331 CRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELSRA 390
           C++H+IWEW+++    +R I +++ D+T+HTHHSAWHSMHNVICGG+K+ D +L+ L ++
Sbjct: 306 CKHHKIWEWLIKLCIGKREIHWKIKDITRHTHHSAWHSMHNVICGGSKVADEHLETLIKS 365

Query: 391 GVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELTQDLEYIWA 441
           GVKI++  GD D + P  CS N+K+      V+++   DH ++I GR  E  + LE IW 
Sbjct: 366 GVKIHLMQGDCDQIVPSHCSGNMKRTFPAVEVDIITGADHDSMISGRGEEFAEKLESIWC 418

BLAST of Cp4.1LG03g03580 vs. TAIR 10
Match: AT5G17720.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 382.5 bits (981), Expect = 4.7e-106
Identity = 195/424 (45.99%), Postives = 268/424 (63.21%), Query Frame = 0

Query: 41  NFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNADGDGENELSETLCGR 100
           ++ VF   D LD  LC+++R++D+ +E K+ +C+C +  E+   A+ +    LSETL  R
Sbjct: 19  SWAVFFLFDLLDYFLCIVFRFLDEVMEEKSESCHCNNPREKTHFAEYE---FLSETLYRR 78

Query: 101 RNVFRRIALVGFSGSCKDSEK----------------MNNGS--AWNRWSDCGCSSCVDG 160
           RNVFR+   + F+    +  K                MN  S    NRWSDCGC +CV  
Sbjct: 79  RNVFRQAGFLRFANKLPEITKKIGIVTFLRKFLFPHTMNKVSHEVANRWSDCGCKTCVSW 138

Query: 161 MENRNQKLYVAVREP---PRGRSGKPKENVIFLHGFVSSSSLWTESVFPNLSE-TQKLNY 220
           +     KL V V++P       S KP ENVIF+HGF++SSS WT +VF  L E T+  NY
Sbjct: 139 I--NTDKLNVIVKQPSISDLSISNKPVENVIFVHGFLASSSFWTNTVFKYLPETTEGTNY 198

Query: 221 RLFAVDLLGFGRSPKPRDCFYTMKDHLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAA 280
           R FA+DLLGFG SPKPR   Y++K+H+E IE+SVI    L SFH+VAHSMGC+I +ALAA
Sbjct: 199 RFFAIDLLGFGDSPKPRASQYSLKEHVEMIEKSVILPNNLTSFHVVAHSMGCIIGIALAA 258

Query: 281 KYSNSVKSITLVAPPYF-PSKDGGAMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACF 340
           K+S+SVKS+ LVAPPYF  SK G +   L+ +  K++WPP  F +++M WYEH+GR  C 
Sbjct: 259 KFSDSVKSVALVAPPYFADSKGGASCAALDVVAKKKLWPPASFFTAMMCWYEHIGRGVCL 318

Query: 341 VICRNHRIWEWILRRINPRRNIDFRVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDELS 400
           V CR+HR WE I++ +  RR +   ++D TKHTH S WHSMHNVICGGAK TD +L+ L 
Sbjct: 319 VFCRHHRTWERIIKIVTWRRKLPTAIMDFTKHTHQSGWHSMHNVICGGAKFTDKHLETLI 378

Query: 401 RAGVKIYIYHGDQDVVAPVECSYNLKKKAVDATVNMVQNVDHQTIILGRERELTQDLEYI 442
           ++GVKI +  GD+DVV P++C  N+K K     V ++   DH T+I+ R      +L  +
Sbjct: 379 KSGVKINVMQGDKDVVVPIDCLSNMKGKFPAVEVEVIAGTDHSTVIMSRREVFAANLVSL 437

BLAST of Cp4.1LG03g03580 vs. TAIR 10
Match: AT1G64670.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 347.1 bits (889), Expect = 2.2e-95
Identity = 176/458 (38.43%), Postives = 265/458 (57.86%), Query Frame = 0

Query: 32  FRTSLLDFVNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYC------------GSRG 91
           F  SL   V   VF  LD +D +LC  Y+ +D F E +   CYC            G+RG
Sbjct: 3   FSRSLNRTVGVFVFFILDIVDFLLCFTYKTLDFFFESEWKPCYCCPPPEAKPISAGGNRG 62

Query: 92  EERGNADGDGE--------------NELSETLCGRRNVFRRIALV------GFSGSCKDS 151
            +   ++  G+              +E+S+TL  R ++  ++  +           C +S
Sbjct: 63  GKMIVSERSGDYSKVVSLTRTKIYLDEISDTLYSRPSLLTKLTKLVKCFKKDVVKCCDES 122

Query: 152 EKMNNGSAWN----------------RWSDCGCSSCVDGMENRNQKLYVAVREPPRGRSG 211
           +K +  +                   RWSDC C+ C   + + NQ L+V V++P      
Sbjct: 123 KKRSPSTKKTLLTVNSTVVEKLQRTPRWSDCHCTFCTSWLSSSNQSLFVNVQQP---TDN 182

Query: 212 KPKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKD 271
           K +ENV+F+HGF+SSS+ WTE++FPN S++ K NYR  AVDLLG+G+SPKP D  YT+K+
Sbjct: 183 KAQENVVFIHGFLSSSTFWTETLFPNFSDSAKSNYRFLAVDLLGYGKSPKPNDSLYTLKE 242

Query: 272 HLEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--G 331
           HLE IE SVI QF L +FHLVAHS+GC++ALALA K+  ++KS+TL+APPY+    G  G
Sbjct: 243 HLEMIERSVISQFRLKTFHLVAHSLGCILALALAVKHPGAIKSLTLLAPPYYSVPKGVQG 302

Query: 332 AMTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDF 391
              V+  L  K VWPP+ FG+SV SWYEH+ R    V+C+NH + E++ R +   R   +
Sbjct: 303 TQYVMRRLAPKEVWPPMAFGASVASWYEHISRTVSLVLCKNHHLLEFLTRLLTRNRMRTY 362

Query: 392 RVIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPVECSY 439
            +     HTH+++WH++HN+I G     + YLD +      ++ ++HG +D + PVECSY
Sbjct: 363 LIEGFLCHTHNASWHTLHNIIFGSGSKVEAYLDHVRDNVDCEVAVFHGGRDELIPVECSY 422

BLAST of Cp4.1LG03g03580 vs. TAIR 10
Match: AT5G41900.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 335.1 bits (858), Expect = 8.6e-92
Identity = 177/460 (38.48%), Postives = 266/460 (57.83%), Query Frame = 0

Query: 40  VNFTVFLFLDFLDAILCVIYRYIDQFLEGKTTACYCGSRGEERGNAD--------GDGE- 99
           V F VF  LD  D +LC  Y+ +D FLE +   CYC S  E +   +        G  + 
Sbjct: 11  VGFFVFALLDIADFLLCYTYKTLDYFLESERKPCYCSSPPEAKAKTEKIIVSERGGYSKV 70

Query: 100 ----------NELSETLCGR--------RNVFRRIALVGFSG---------SC---KDSE 159
                     +E+S+TL  R          + R +    + G         SC   +  +
Sbjct: 71  VSLTRSKIHFDEISDTLYSRGPSLLTRLSKLVRSVKCFNYKGLIMRGNVVESCDHHESKK 130

Query: 160 KMNNG---------------SAWNRWSDCGCSSCVDGMENRNQ-KLYVAVREPPRGRSGK 219
           K++ G               S   RWSDC CS C   + + N+  L+V V++P   +  K
Sbjct: 131 KISKGKKRLMTLNSTVIEKSSTAPRWSDCHCSFCTSWLTSTNRDSLFVKVQQPKDNK--K 190

Query: 220 PKENVIFLHGFVSSSSLWTESVFPNLSETQKLNYRLFAVDLLGFGRSPKPRDCFYTMKDH 279
            ++NV+F+HGFVSSS+ WTE++FPN S++ K NYR  AVDLLG+GRSPKP D  YT+++H
Sbjct: 191 ARDNVVFIHGFVSSSAFWTETLFPNFSDSAKSNYRFIAVDLLGYGRSPKPNDSLYTLREH 250

Query: 280 LEKIEESVIHQFGLNSFHLVAHSMGCLIALALAAKYSNSVKSITLVAPPYFPSKDG--GA 339
           LE IE+SVI +F L +FH+VAHS+GC++ALALA K+  ++KS+TL+APPY+    G   A
Sbjct: 251 LEMIEKSVISKFKLKTFHIVAHSLGCILALALAVKHPGAIKSLTLLAPPYYKVPKGVQPA 310

Query: 340 MTVLENLGAKRVWPPLLFGSSVMSWYEHVGRCACFVICRNHRIWEWILRRINPRRNIDFR 399
             V+  +  K VWPP+ FG+SV+SWYEH+GR    V+ +NH++ E++ R +   R   + 
Sbjct: 311 QYVMREVARKEVWPPMQFGASVLSWYEHLGRTIGLVLIKNHQLIEFVTRLLTLNRMRTYL 370

Query: 400 VIDLTKHTHHSAWHSMHNVICGGAKLTDGYLDEL-SRAGVKIYIYHGDQDVVAPVECSYN 442
           +     HTH+ ++H++HN+I G     D YLD +       + I+HG +D + PVECSY+
Sbjct: 371 IEGFLCHTHNGSFHTLHNIIFGSGAKLDSYLDHVRDHVDCDVAIFHGGKDELIPVECSYS 430