Cp4.1LG03g03190 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g03190
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cp4.1LG03: 273490 .. 274840 (+)
RNA-Seq Expression	Cp4.1LG03g03190
Synteny	Cp4.1LG03g03190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAGTCTTAAAAATCCACAATTGCATCAGAAAATAGATAAGATAAGATAAAGAAAATGAGTTGGCCGGAGCCGGTGGTTTGTGTGCAGTCGCTGGCGGAGAGTGGAATAAGGACGATTCCAGTGAGGTATGTGAAGCCGCCATGTCAGAGGCCAGGTGGGGCCGCCATGGAGAGTGTTGAGGTGCAGAGGAATATTCCGGTGGTGGACATGGCGAAGCTGGAGAGCGCGGCGGCGGTGAGGGAGATGGCGGAAGCTTGCCGGGAATGGGGATTTTTCCATGTAATAAACCATGGGATTAGTGGTGAGGTGATGGAGTGCGTGAAGGAGTCATGGAAACAGTTCTTCGATCAGCCGTTGGATTTGAAGAAACAATATGCCAACTCGCCCATGACTTATGAAGGATACGGCAGCCGTTTGGGTATCGAAAAGGGCGCCATATTGGATTGGAGTGATTACTTTTTCTTGAACTATATGCCCACTTCCCTCAGAAACCCAGCCATGTGGCCTGCCTTCCCTCCCTCCTCCAAGTGCGTCTCAAAATCATACTATTCAAAGTATTGATATGATTAAATTTAACGTAATCTATCGACTTAAATTTTTATTTTGATCGGCATTTAACCGTTTTATGATTTTAAGCTAACGAAGGTCGGGTTGTGGTCGACAGGAAATTGATAGAAGAATATGGGAACGAGGCGGTGAAGCTATGTGGGAAACTAATGAAGAGGTTATCAGTGGGATTGGGGCTTAAAGAAGAGTATTTATTGAATGCATTTGGAGGAGAAGAGGGAATAGGGGCTTGCATGAGAGCCAATATGTATCCCAAATGCCCACAGCCCGACCTGACTTTAGGCCTTTCCCCTCACTCGGACCCTGGCGGCATCACCATTCTCTTACCCGACCACAACGTTCCTGGCCTCCAAGTTCGCAAGGGCGACCATTGGATCACTATCGACCCTATTCCTAATGCTTTGATTGTCAATATCGGTGATCAAATTCAGGTCCGTTCGATCTTTTTCTCTCTTTCGAATTTCAAACAATCTAATATGTTTCAATCTTGCATGGTTGACTGATACGACTATATATGTTTTGTTGATTAGGTGTTGAGCAATGCAATATACAAGAGCGTGAAACATAGGGTGATCGTAAATTCAAACAAGGATCGAGTGTCCTTGGCCTTCTTCTACAACCCTAAAAGTGATTTGATAATTGAACCAGCCAAGGAGCTTGTCAGTGAAGAAAGGCCAGCCCTTTTTCCACGCATGACCTTCGATGAATATAGACTGCACATTAGGAAGAGGGGTATTTGTGGAACTTCACAGCATGAACAATCTCTTGAATCCACTTAA

mRNA sequence

ATGTGGAATAAGATAAAGAAAATGAGTTGGCCGGAGCCGGTGGTTTGTGTGCAGTCGCTGGCGGAGAGTGGAATAAGGACGATTCCAGTGAGGTATGTGAAGCCGCCATGTCAGAGGCCAGGTGGGGCCGCCATGGAGAGTGTTGAGGTGCAGAGGAATATTCCGGTGGTGGACATGGCGAAGCTGGAGAGCGCGGCGGCGGTGAGGGAGATGGCGGAAGCTTGCCGGGAATGGGGATTTTTCCATGTAATAAACCATGGGATTAGTGGTGAGGTGATGGAGTGCGTGAAGGAGTCATGGAAACAGTTCTTCGATCAGCCGTTGGATTTGAAGAAACAATATGCCAACTCGCCCATGACTTATGAAGGATACGGCAGCCGTTTGGGTATCGAAAAGGGCGCCATATTGGATTGGATGGGATTGGGGCTTAAAGAAGAGTATTTATTGAATGCATTTGGAGGAGAAGAGGGAATAGGGGCTTGCATGAGAGCCAATATGTATCCCAAATGCCCACAGCCCGACCTGACTTTAGGCCTTTCCCCTCACTCGGACCCTGGCGGCATCACCATTCTCTTACCCGACCACAACGTTCCTGGCCTCCAAGTTCGCAAGGGCGACCATTGGATCACTATCGACCCTATTCCTAATGCTTTGATTGTCAATATCGGTGATCAAATTCAGGTGTTGAGCAATGCAATATACAAGAGCGTGAAACATAGGGTGATCGTAAATTCAAACAAGGATCGAGTGTCCTTGGCCTTCTTCTACAACCCTAAAAGTGATTTGATAATTGAACCAGCCAAGGAGCTTGTCAGTGAAGAAAGGCCAGCCCTTTTTCCACGCATGACCTTCGATGAATATAGACTGCACATTAGGAAGAGGGGTATTTGTGGAACTTCACAGCATGAACAATCTCTTGAATCCACTTAA

Coding sequence (CDS)

ATGTGGAATAAGATAAAGAAAATGAGTTGGCCGGAGCCGGTGGTTTGTGTGCAGTCGCTGGCGGAGAGTGGAATAAGGACGATTCCAGTGAGGTATGTGAAGCCGCCATGTCAGAGGCCAGGTGGGGCCGCCATGGAGAGTGTTGAGGTGCAGAGGAATATTCCGGTGGTGGACATGGCGAAGCTGGAGAGCGCGGCGGCGGTGAGGGAGATGGCGGAAGCTTGCCGGGAATGGGGATTTTTCCATGTAATAAACCATGGGATTAGTGGTGAGGTGATGGAGTGCGTGAAGGAGTCATGGAAACAGTTCTTCGATCAGCCGTTGGATTTGAAGAAACAATATGCCAACTCGCCCATGACTTATGAAGGATACGGCAGCCGTTTGGGTATCGAAAAGGGCGCCATATTGGATTGGATGGGATTGGGGCTTAAAGAAGAGTATTTATTGAATGCATTTGGAGGAGAAGAGGGAATAGGGGCTTGCATGAGAGCCAATATGTATCCCAAATGCCCACAGCCCGACCTGACTTTAGGCCTTTCCCCTCACTCGGACCCTGGCGGCATCACCATTCTCTTACCCGACCACAACGTTCCTGGCCTCCAAGTTCGCAAGGGCGACCATTGGATCACTATCGACCCTATTCCTAATGCTTTGATTGTCAATATCGGTGATCAAATTCAGGTGTTGAGCAATGCAATATACAAGAGCGTGAAACATAGGGTGATCGTAAATTCAAACAAGGATCGAGTGTCCTTGGCCTTCTTCTACAACCCTAAAAGTGATTTGATAATTGAACCAGCCAAGGAGCTTGTCAGTGAAGAAAGGCCAGCCCTTTTTCCACGCATGACCTTCGATGAATATAGACTGCACATTAGGAAGAGGGGTATTTGTGGAACTTCACAGCATGAACAATCTCTTGAATCCACTTAA

Protein sequence

MWNKIKKMSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAVREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRLGIEKGAILDWMGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPKSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST

Homology

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 361.3 bits (926), Expect = 1.1e-98
Identity = 182/350 (52.00%), Postives = 229/350 (65.43%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVE--VQRNIPVVDMAKLESAAA 69
           WPEP+V VQSLAES + ++P RY+KPP QRP    ++        NIP++D+  L S   
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 70  --VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYG 129
              + ++EACREWGFF VINHG+  E+M+  +E+WK FF+ P++ K+ Y+NSP TYEGYG
Sbjct: 109 DDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYG 168

Query: 130 SRLGIEKGAILDW----------------------------------------------- 189
           SRLG+EKGAILDW                                               
Sbjct: 169 SRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTI 228

Query: 190 --MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 249
               LGL+ E L  AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD  
Sbjct: 229 LSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQ 288

Query: 250 VPGLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFY 307
           V GLQVR GD WIT++P+ +A IVNIGDQIQ+LSN+ YKSV+HRVIVNS K+RVSLAFFY
Sbjct: 289 VVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFY 348

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 1.3e-96
Identity = 180/352 (51.14%), Postives = 224/352 (63.64%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAVR 69
           WPEP+V VQSLAES + ++P RY+KP   RP     E      NIP++D+  L S   + 
Sbjct: 20  WPEPIVRVQSLAESNLSSLPDRYIKPASLRP--TTTEDAPTATNIPIIDLEGLFSEEGLS 79

Query: 70  E------MAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEG 129
           +      ++EACR WGFF V+NHG+  E+M+  +E+W++FF  P++ K+ Y+NSP TYEG
Sbjct: 80  DDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEG 139

Query: 130 YGSRLGIEKGAILDW--------------------------------------------- 189
           YGSRLG+EKGA LDW                                             
Sbjct: 140 YGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIM 199

Query: 190 ----MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 249
                 LGLKE+    AFGG E IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILLPD
Sbjct: 200 RVLSTNLGLKEDKFQEAFGG-ENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPD 259

Query: 250 HNVPGLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAF 307
             V GLQVRK D WIT+ P P+A IVNIGDQIQ+LSN+ YKSV+HRVIVNS+K+RVSLAF
Sbjct: 260 DQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAF 319

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 8.9e-93
Identity = 173/347 (49.86%), Postives = 222/347 (63.98%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKL-ESAAAV 69
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     +S +    IPV+DM  +      +
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQS-DAGIEIPVLDMNDVWGKPEGL 64

Query: 70  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 129
           R +  AC EWGFF ++NHG++  +ME V+ +W++FF+ PL+ K++YANSP TYEGYGSRL
Sbjct: 65  RLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRL 124

Query: 130 GIEKGAILDWM------------------------------------------------- 189
           G+ K A LDW                                                  
Sbjct: 125 GVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSE 184

Query: 190 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 249
            LGLK   L+ A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V G
Sbjct: 185 SLGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAG 244

Query: 250 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 307
           LQVR+GD W+TI  +PNALIVNIGDQ+Q+LSN IYKSV+H+VIVNS  +RVSLAFFYNP+
Sbjct: 245 LQVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPR 304

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.1e-90
Identity = 171/343 (49.85%), Postives = 219/343 (63.85%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQ-RNIPVVDMAKLES---- 69
           WPEP+V VQSL+ES +  IP RYVKP  QRP          Q   IP++D+ +L +    
Sbjct: 7   WPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLT 66

Query: 70  --AAAVREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYE 129
             A  + E+++ACRE GFF V+NHG+S ++M+  K +W++FF+ P++LK  +ANSP TYE
Sbjct: 67  LQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHANSPKTYE 126

Query: 130 GYGSRLGIEKGAILDW-------------------------------------------- 189
           GYGSRLG+EKGAILDW                                            
Sbjct: 127 GYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCENL 186

Query: 190 -----MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLP 249
                  LGL+E+ L NAFGG+E  G C+R N YPKCPQP+LTLG+SPHSDPGG+TILLP
Sbjct: 187 MKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTILLP 246

Query: 250 DHNVPGLQVR-KGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSL 296
           D  V  LQVR   D WIT++P P+A IVN+GDQIQ+LSN+IYKSV+HRVIVN   +R+SL
Sbjct: 247 DEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPENERLSL 306

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy Swiss-Prot
Match: Q39224 (Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 2.7e-49
Identity = 107/334 (32.04%), Postives = 171/334 (51.20%), Query Frame = 0


Query: 18  QSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESA----AAVREMAE 77
           + + E  I T+P RYV+    +      +  +V+  IP++DM +L S+    + V ++  
Sbjct: 19  EMVKEKTITTVPPRYVRSDQDKT--EVDDDFDVKIEIPIIDMKRLCSSTTMDSEVEKLDF 78

Query: 78  ACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRLGIEKG 137
           AC+EWGFF ++NHGI    ++ VK   + FF+ P++ KK++   P   EG+G    + + 
Sbjct: 79  ACKEWGFFQLVNHGIDSSFLDKVKSEIQDFFNLPMEEKKKFWQRPDEIEGFGQAFVVSED 138

Query: 138 AILDW-------------------------------------------------MGLGLK 197
             LDW                                                   L +K
Sbjct: 139 QKLDWADLFFHTVQPVELRKPHLFPKLPLPFRDTLEMYSSEVQSVAKILIAKMARALEIK 198

Query: 198 EEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGLQVRK 257
            E L   F   + + + MR N YP CPQPD  +GL+PHSD  G+T+L+  ++V GLQ++K
Sbjct: 199 PEELEKLFDDVDSVQS-MRMNYYPPCPQPDQVIGLTPHSDSVGLTVLMQVNDVEGLQIKK 258

Query: 258 GDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPKSDLII 299
              W+ + P+PNA IVNIGD +++++N  Y+S++HR +VNS K+R+S+A F+N      +
Sbjct: 259 DGKWVPVKPLPNAFIVNIGDVLEIITNGTYRSIEHRGVVNSEKERLSIATFHNVGMYKEV 318

[Close]

BLAST of Cp4.1LG03g03190 vs. NCBI nr
Match: XP_023528714.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597 bits (1539), Expect = 3.43e-214
Identity = 301/351 (85.75%), Postives = 302/351 (86.04%), Query Frame = 0


Query: 8   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA 67
           MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA
Sbjct: 1   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA 60

Query: 68  VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 127
           VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR
Sbjct: 61  VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 120

Query: 128 LGIEKGAILDW------------------------------------------------- 187
           LGIEKGAILDW                                                 
Sbjct: 121 LGIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGNEAVKLCGKLMKRLS 180

Query: 188 MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVP 247
           +GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVP
Sbjct: 181 VGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVP 240

Query: 248 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 307
           GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP
Sbjct: 241 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 300

Query: 308 KSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST 309
           KSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST
Sbjct: 301 KSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST 351

[Close]

BLAST of Cp4.1LG03g03190 vs. NCBI nr
Match: XP_022935289.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata])

HSP 1 Score: 587 bits (1514), Expect = 2.21e-210
Identity = 296/351 (84.33%), Postives = 300/351 (85.47%), Query Frame = 0


Query: 8   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA 67
           MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQ NIPVVDMAKLESAAA
Sbjct: 1   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQMNIPVVDMAKLESAAA 60

Query: 68  VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 127
           VR+MAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR
Sbjct: 61  VRDMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 120

Query: 128 LGIEKGAILDW------------------------------------------------- 187
           LGIEKGAILDW                                                 
Sbjct: 121 LGIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSTKKLIEEYGNEVVKLCGKLMKRLS 180

Query: 188 MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVP 247
           +GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVP
Sbjct: 181 VGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVP 240

Query: 248 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 307
           GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP
Sbjct: 241 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 300

Query: 308 KSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST 309
           KSDLIIEPAKELVSEERPALFPRMTFDEYRL IRKRGICGTSQHEQSL+ST
Sbjct: 301 KSDLIIEPAKELVSEERPALFPRMTFDEYRLDIRKRGICGTSQHEQSLKST 351

[Close]

BLAST of Cp4.1LG03g03190 vs. NCBI nr
Match: XP_022983369.1 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita maxima])

HSP 1 Score: 584 bits (1506), Expect = 3.66e-209
Identity = 292/351 (83.19%), Postives = 302/351 (86.04%), Query Frame = 0


Query: 8   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA 67
           MSWPEPVVCVQSLAESGI+TIPVRYVKPPCQRPGGAAMESVEVQRNIP+VDMAKLESAAA
Sbjct: 1   MSWPEPVVCVQSLAESGIKTIPVRYVKPPCQRPGGAAMESVEVQRNIPMVDMAKLESAAA 60

Query: 68  VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 127
           VR+MAEAC+EWGFFHVINHGISGE+MECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR
Sbjct: 61  VRDMAEACQEWGFFHVINHGISGELMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 120

Query: 128 LGIEKGAILDW------------------------------------------------- 187
           LGIEKGAILDW                                                 
Sbjct: 121 LGIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGSEVVKLCGKLMKRLS 180

Query: 188 MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVP 247
           +GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVP
Sbjct: 181 VGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVP 240

Query: 248 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 307
           GLQVRKGD+WITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP
Sbjct: 241 GLQVRKGDNWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 300

Query: 308 KSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST 309
           KSDLIIEPAKELV+EERPALFPRMTFDEYRLHIRKRGICGTSQHEQSL+ST
Sbjct: 301 KSDLIIEPAKELVTEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLKST 351

[Close]

BLAST of Cp4.1LG03g03190 vs. NCBI nr
Match: KAG7017221.1 (putative 2-oxoglutarate-dependent dioxygenase0, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 578 bits (1489), Expect = 2.08e-207
Identity = 283/302 (93.71%), Postives = 288/302 (95.36%), Query Frame = 0


Query: 8   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA 67
           MSWPEPVVCVQSL     +       +PPCQRPGGAAMESVEVQ NIPVVDMAKLESAAA
Sbjct: 1   MSWPEPVVCVQSLGGEWNKD---DSSEPPCQRPGGAAMESVEVQMNIPVVDMAKLESAAA 60

Query: 68  VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 127
           VR+MAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR
Sbjct: 61  VRDMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 120

Query: 128 LGIEKGAILDWMGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 187
           LGIEKGAILDWMGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG
Sbjct: 121 LGIEKGAILDWMGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 180

Query: 188 ITILLPDHNVPGLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNK 247
           +TILLPDHNVPGLQV KGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNK
Sbjct: 181 MTILLPDHNVPGLQVHKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNK 240

Query: 248 DRVSLAFFYNPKSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLE 307
           DRVSLAFFYNPKSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSL+
Sbjct: 241 DRVSLAFFYNPKSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLK 299

Query: 308 ST 309
           ST
Sbjct: 301 ST 299

[Close]

BLAST of Cp4.1LG03g03190 vs. NCBI nr
Match: XP_038903719.1 (probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida])

HSP 1 Score: 517 bits (1332), Expect = 1.20e-182
Identity = 261/350 (74.57%), Postives = 279/350 (79.71%), Query Frame = 0


Query: 9   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAV 68
           SWPEPVVCVQSLAESGIRTIP+RYVKPP QRPGG  ++     RNIPVVDM KLES AAV
Sbjct: 7   SWPEPVVCVQSLAESGIRTIPMRYVKPPSQRPGGDGVQ----MRNIPVVDMEKLESGAAV 66

Query: 69  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 128
           RE AEACREWGFF +INHGISGE+MECVK++WK+FFDQPLDLKKQYANSP TYEGYGSRL
Sbjct: 67  RETAEACREWGFFQLINHGISGEMMECVKDTWKEFFDQPLDLKKQYANSPATYEGYGSRL 126

Query: 129 GIEKGAILDW-------------------------------------------------M 188
           GIEKGAILDW                                                 +
Sbjct: 127 GIEKGAILDWSDYFFLNFMPFSLRNPAIWPAFPPSSKKLIEEYGDEVMKLCGKLMKGLSL 186

Query: 189 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 248
           GLGL+EEYLL AFG E+GIG+CMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG
Sbjct: 187 GLGLEEEYLLKAFGEEKGIGSCMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 246

Query: 249 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 308
           LQV KG  WITIDPIPNALIVN+GDQIQVLSN IYKSVKHRVIVNSNKDRVSLAFFYNPK
Sbjct: 247 LQVLKGHDWITIDPIPNALIVNVGDQIQVLSNGIYKSVKHRVIVNSNKDRVSLAFFYNPK 306

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy TrEMBL
Match: A0A6J1F503 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111442217 PE=3 SV=1)

HSP 1 Score: 587 bits (1514), Expect = 1.07e-210
Identity = 296/351 (84.33%), Postives = 300/351 (85.47%), Query Frame = 0


Query: 8   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA 67
           MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQ NIPVVDMAKLESAAA
Sbjct: 1   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQMNIPVVDMAKLESAAA 60

Query: 68  VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 127
           VR+MAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR
Sbjct: 61  VRDMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 120

Query: 128 LGIEKGAILDW------------------------------------------------- 187
           LGIEKGAILDW                                                 
Sbjct: 121 LGIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSTKKLIEEYGNEVVKLCGKLMKRLS 180

Query: 188 MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVP 247
           +GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVP
Sbjct: 181 VGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVP 240

Query: 248 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 307
           GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP
Sbjct: 241 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 300

Query: 308 KSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST 309
           KSDLIIEPAKELVSEERPALFPRMTFDEYRL IRKRGICGTSQHEQSL+ST
Sbjct: 301 KSDLIIEPAKELVSEERPALFPRMTFDEYRLDIRKRGICGTSQHEQSLKST 351

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy TrEMBL
Match: A0A6J1J756 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3661 GN=LOC111481976 PE=3 SV=1)

HSP 1 Score: 584 bits (1506), Expect = 1.77e-209
Identity = 292/351 (83.19%), Postives = 302/351 (86.04%), Query Frame = 0


Query: 8   MSWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAA 67
           MSWPEPVVCVQSLAESGI+TIPVRYVKPPCQRPGGAAMESVEVQRNIP+VDMAKLESAAA
Sbjct: 1   MSWPEPVVCVQSLAESGIKTIPVRYVKPPCQRPGGAAMESVEVQRNIPMVDMAKLESAAA 60

Query: 68  VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 127
           VR+MAEAC+EWGFFHVINHGISGE+MECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR
Sbjct: 61  VRDMAEACQEWGFFHVINHGISGELMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSR 120

Query: 128 LGIEKGAILDW------------------------------------------------- 187
           LGIEKGAILDW                                                 
Sbjct: 121 LGIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGSEVVKLCGKLMKRLS 180

Query: 188 MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVP 247
           +GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVP
Sbjct: 181 VGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVP 240

Query: 248 GLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 307
           GLQVRKGD+WITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP
Sbjct: 241 GLQVRKGDNWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNP 300

Query: 308 KSDLIIEPAKELVSEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLEST 309
           KSDLIIEPAKELV+EERPALFPRMTFDEYRLHIRKRGICGTSQHEQSL+ST
Sbjct: 301 KSDLIIEPAKELVTEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSLKST 351

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy TrEMBL
Match: A0A6J1G9Z8 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita moschata OX=3662 GN=LOC111452304 PE=3 SV=1)

HSP 1 Score: 516 bits (1328), Expect = 2.20e-182
Identity = 260/346 (75.14%), Postives = 275/346 (79.48%), Query Frame = 0


Query: 9   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAV 68
           SWPEPVVCVQSLAESGIRTIPVRYVKPP QRP GAA+E   V RNIPVVDM KL+SAA V
Sbjct: 6   SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVER-NVPRNIPVVDMEKLQSAAEV 65

Query: 69  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 128
           + MAEAC+EWGFFHVINHGI+GE+MEC+K+ W QFF QPLDLK+QYANSP TYEGYGSRL
Sbjct: 66  KAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRL 125

Query: 129 GIEKGAILDW------------------------------------------------MG 188
           GIEK AILDW                                                MG
Sbjct: 126 GIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKGLSMG 185

Query: 189 LGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 248
           LGL+EEYLL AFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL
Sbjct: 186 LGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 245

Query: 249 QVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPKS 306
           QVRKGD WITIDPIPNALIVN+ DQIQVLSN IYKSV HRVIVNSNKDRVSLAFFYNP+S
Sbjct: 246 QVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPRS 305

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy TrEMBL
Match: A0A6J1I649 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN=LOC111470253 PE=3 SV=1)

HSP 1 Score: 514 bits (1323), Expect = 1.27e-181
Identity = 260/346 (75.14%), Postives = 274/346 (79.19%), Query Frame = 0


Query: 9   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAV 68
           SWPEPVVCVQSLAESGIRTIPVRYVKPP QRP GAA++   V RNIPVVDM KL+SAA V
Sbjct: 6   SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVKR-NVWRNIPVVDMEKLQSAAEV 65

Query: 69  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 128
             MAEAC+EWGFFHVINHGI+GE+MEC+K+ W QFF QPLDLK+QYANSP TYEGYGSRL
Sbjct: 66  TAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRL 125

Query: 129 GIEKGAILDW------------------------------------------------MG 188
           GIEKGAILDW                                                MG
Sbjct: 126 GIEKGAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKGLSMG 185

Query: 189 LGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 248
           LGL+EEYLL AFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL
Sbjct: 186 LGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 245

Query: 249 QVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPKS 306
           QVRKGD WITIDPIPNALIVN+ DQIQVLSN IYKSV HRVIVNSNKDRVSLAFFYNP+S
Sbjct: 246 QVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPRS 305

[Close]

BLAST of Cp4.1LG03g03190 vs. ExPASy TrEMBL
Match: A0A0A0LCS8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731160 PE=3 SV=1)

HSP 1 Score: 488 bits (1257), Expect = 1.37e-171
Identity = 245/345 (71.01%), Postives = 270/345 (78.26%), Query Frame = 0


Query: 9   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAV 68
           SWPEPVVCVQSLAESGI  IP RYVKPP QRP G    +V+  RNIPVVDM K+ES AAV
Sbjct: 8   SWPEPVVCVQSLAESGISKIPGRYVKPPSQRPDGG---TVKKMRNIPVVDMEKVESGAAV 67

Query: 69  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 128
           +EMAEACREWGFF +INHGISGE++ECVKESWK+FF+QPLDLK QYAN+P TYEGYGSRL
Sbjct: 68  KEMAEACREWGFFQIINHGISGEMIECVKESWKEFFNQPLDLKNQYANTPATYEGYGSRL 127

Query: 129 GIEKGAILDW-------------------------------------------------M 188
           GIEKGAILDW                                                 M
Sbjct: 128 GIEKGAILDWSDYFFLNFLPLSLRNPTKWPAFPSSFKKVIEEYGDEVVKVCAKLMKGLSM 187

Query: 189 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 248
           GLGL+EEYLLNAFGGE+GIG  MRANMYPKCPQPDLTLGLS HSDPGGITILL DHNVPG
Sbjct: 188 GLGLEEEYLLNAFGGEKGIGGSMRANMYPKCPQPDLTLGLSSHSDPGGITILLADHNVPG 247

Query: 249 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 304
           LQV KG+ WIT+DPIPNALIVNIGDQIQVLSN IYKSVKHRV+VN NK+RVSLAFFYNPK
Sbjct: 248 LQVLKGNDWITVDPIPNALIVNIGDQIQVLSNGIYKSVKHRVMVNPNKERVSLAFFYNPK 307

[Close]

BLAST of Cp4.1LG03g03190 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 361.3 bits (926), Expect = 7.7e-100
Identity = 182/350 (52.00%), Postives = 229/350 (65.43%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVE--VQRNIPVVDMAKLESAAA 69
           WPEP+V VQSLAES + ++P RY+KPP QRP    ++        NIP++D+  L S   
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 70  --VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYG 129
              + ++EACREWGFF VINHG+  E+M+  +E+WK FF+ P++ K+ Y+NSP TYEGYG
Sbjct: 109 DDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYG 168

Query: 130 SRLGIEKGAILDW----------------------------------------------- 189
           SRLG+EKGAILDW                                               
Sbjct: 169 SRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTI 228

Query: 190 --MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 249
               LGL+ E L  AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD  
Sbjct: 229 LSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQ 288

Query: 250 VPGLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFY 307
           V GLQVR GD WIT++P+ +A IVNIGDQIQ+LSN+ YKSV+HRVIVNS K+RVSLAFFY
Sbjct: 289 VVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFY 348

[Close]

BLAST of Cp4.1LG03g03190 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 358.6 bits (919), Expect = 5.0e-99
Identity = 179/339 (52.80%), Postives = 225/339 (66.37%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVE--VQRNIPVVDMAKLESAAA 69
           WPEP+V VQSLAES + ++P RY+KPP QRP    ++        NIP++D+  L S   
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 70  --VREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYG 129
              + ++EACREWGFF VINHG+  E+M+  +E+WK FF+ P++ K+ Y+NSP TYEGYG
Sbjct: 109 DDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYG 168

Query: 130 SRLGIEKGAILDW----------------------------------------------- 189
           SRLG+EKGAILDW                                               
Sbjct: 169 SRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTI 228

Query: 190 --MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 249
               LGL+ E L  AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD  
Sbjct: 229 LSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQ 288

Query: 250 VPGLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFY 296
           V GLQVR GD WIT++P+ +A IVNIGDQIQ+LSN+ YKSV+HRVIVNS K+RVSLAFFY
Sbjct: 289 VVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFY 348

[Close]

BLAST of Cp4.1LG03g03190 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 354.4 bits (908), Expect = 9.4e-98
Identity = 180/352 (51.14%), Postives = 224/352 (63.64%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKLESAAAVR 69
           WPEP+V VQSLAES + ++P RY+KP   RP     E      NIP++D+  L S   + 
Sbjct: 20  WPEPIVRVQSLAESNLSSLPDRYIKPASLRP--TTTEDAPTATNIPIIDLEGLFSEEGLS 79

Query: 70  E------MAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEG 129
           +      ++EACR WGFF V+NHG+  E+M+  +E+W++FF  P++ K+ Y+NSP TYEG
Sbjct: 80  DDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTYEG 139

Query: 130 YGSRLGIEKGAILDW--------------------------------------------- 189
           YGSRLG+EKGA LDW                                             
Sbjct: 140 YGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIM 199

Query: 190 ----MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPD 249
                 LGLKE+    AFGG E IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILLPD
Sbjct: 200 RVLSTNLGLKEDKFQEAFGG-ENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPD 259

Query: 250 HNVPGLQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAF 307
             V GLQVRK D WIT+ P P+A IVNIGDQIQ+LSN+ YKSV+HRVIVNS+K+RVSLAF
Sbjct: 260 DQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAF 319

[Close]

BLAST of Cp4.1LG03g03190 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 341.7 bits (875), Expect = 6.3e-94
Identity = 173/347 (49.86%), Postives = 222/347 (63.98%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQRNIPVVDMAKL-ESAAAV 69
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     +S +    IPV+DM  +      +
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQS-DAGIEIPVLDMNDVWGKPEGL 64

Query: 70  REMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 129
           R +  AC EWGFF ++NHG++  +ME V+ +W++FF+ PL+ K++YANSP TYEGYGSRL
Sbjct: 65  RLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRL 124

Query: 130 GIEKGAILDWM------------------------------------------------- 189
           G+ K A LDW                                                  
Sbjct: 125 GVVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSE 184

Query: 190 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 249
            LGLK   L+ A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V G
Sbjct: 185 SLGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAG 244

Query: 250 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 307
           LQVR+GD W+TI  +PNALIVNIGDQ+Q+LSN IYKSV+H+VIVNS  +RVSLAFFYNP+
Sbjct: 245 LQVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPR 304

[Close]

BLAST of Cp4.1LG03g03190 vs. TAIR 10
Match: AT3G55970.1 (jasmonate-regulated gene 21 )

HSP 1 Score: 334.7 bits (857), Expect = 7.7e-92
Identity = 171/343 (49.85%), Postives = 219/343 (63.85%), Query Frame = 0


Query: 10  WPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQ-RNIPVVDMAKLES---- 69
           WPEP+V VQSL+ES +  IP RYVKP  QRP          Q   IP++D+ +L +    
Sbjct: 7   WPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLT 66

Query: 70  --AAAVREMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYE 129
             A  + E+++ACRE GFF V+NHG+S ++M+  K +W++FF+ P++LK  +ANSP TYE
Sbjct: 67  LQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHANSPKTYE 126

Query: 130 GYGSRLGIEKGAILDW-------------------------------------------- 189
           GYGSRLG+EKGAILDW                                            
Sbjct: 127 GYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCENL 186

Query: 190 -----MGLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLP 249
                  LGL+E+ L NAFGG+E  G C+R N YPKCPQP+LTLG+SPHSDPGG+TILLP
Sbjct: 187 MKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTILLP 246

Query: 250 DHNVPGLQVR-KGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSL 296
           D  V  LQVR   D WIT++P P+A IVN+GDQIQ+LSN+IYKSV+HRVIVN   +R+SL
Sbjct: 247 DEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPENERLSL 306

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g03190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SRM3	1.1e-98	52.00	Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1	[more]
Q9FFF6	1.3e-96	51.14	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
O80449	8.9e-93	49.86	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q9LY48	1.1e-90	49.85	Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1	[more]
Q39224	2.7e-49	32.04	Protein SRG1 OS=Arabidopsis thaliana OX=3702 GN=SRG1 PE=2 SV=1	[more]

BLAST of Cp4.1LG03g03190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023528714.1	3.43e-214	85.75	probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita pepo subsp. p...	[more]
XP_022935289.1	2.21e-210	84.33	probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita moschata]	[more]
XP_022983369.1	3.66e-209	83.19	probable 2-oxoglutarate-dependent dioxygenase At5g05600 [Cucurbita maxima]	[more]
KAG7017221.1	2.08e-207	93.71	putative 2-oxoglutarate-dependent dioxygenase0, partial [Cucurbita argyrosperma ...	[more]
XP_038903719.1	1.20e-182	74.57	probable 2-oxoglutarate-dependent dioxygenase ANS [Benincasa hispida]	[more]

BLAST of Cp4.1LG03g03190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F503	1.07e-210	84.33	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX...	[more]
A0A6J1J756	1.77e-209	83.19	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3...	[more]
A0A6J1G9Z8	2.20e-182	75.14	probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1I649	1.27e-181	75.14	probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN...	[more]
A0A0A0LCS8	1.37e-171	71.01	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]

BLAST of Cp4.1LG03g03190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G11180.1	7.7e-100	52.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.2	5.0e-99	52.80	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G05600.1	9.4e-98	51.14	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G38240.1	6.3e-94	49.86	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G55970.1	7.7e-92	49.85	jasmonate-regulated gene 21	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 140..294 e-value: 4.3E-57 score: 195.8
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 12..139 e-value: 1.1E-28 score: 102.7
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 160..258 e-value: 1.2E-27 score: 96.3
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 54..138 e-value: 6.1E-22 score: 78.6
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 140..305
None	No IPR available	PANTHER	PTHR47990:SF62	LEUCOANTHOCYANIDIN DIOXYGENASE-LIKE PROTEIN	coord: 11..138
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 11..138
None	No IPR available	PANTHER	PTHR47990:SF62	LEUCOANTHOCYANIDIN DIOXYGENASE-LIKE PROTEIN	coord: 140..305
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 14..293
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 157..258 score: 14.511045

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g03190.1	Cp4.1LG03g03190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031347	regulation of defense response
biological_process	GO:0002831	regulation of response to biotic stimulus
biological_process	GO:0032101	regulation of response to external stimulus
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding