Cp4.1LG03g03140 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG03g03140 vs. ExPASy Swiss-Prot
Match: Q9M6N7 (Ammonium transporter 2 OS=Arabidopsis thaliana OX=3702 GN=AMT2 PE=1 SV=2)

HSP 1 Score: 709.1 bits (1829), Expect = 3.3e-203
Identity = 349/473 (73.78%), Postives = 397/473 (83.93%), Query Frame = 0

Query: 15  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 74
           P WLN GDN+WQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL+CWVL
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 75  VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY 134
           + Y+MAFG++L+P WGKG PA  Q YL  +A IP S              P  PMA+LVY
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVA----------APYFPMATLVY 132

Query: 135 FQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYS 194
           FQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IF+YTVGAYS+WGGGFL+QWGVIDYS
Sbjct: 133 FQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYS 192

Query: 195 GGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHS 254
           GGYVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP++
Sbjct: 193 GGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYA 252

Query: 255 ANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGVVQTW 314
           AN+ +SIAVLNTN+SAATSLLVWT LDV++FGKPSVIGAIQGM+TGLA VTPGAG++QTW
Sbjct: 253 ANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTW 312

Query: 315 AAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLC 374
           AA+I+G++ G+ PW SMM++HKKS+ LQK DDTL VF+THAVAG++GG++TGL A P LC
Sbjct: 313 AAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDLC 372

Query: 375 DLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEAL 434
            L LP+  TRGAFYG NGG Q LKQLAG+AFI  WNVVSTTIILL IR+F+PLRM +E L
Sbjct: 373 VLVLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEEL 432

Query: 435 MIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGARGVIIEL 488
            IGDDA HGEEAYALWGDGEKFD  RH       +E     Y+HGARGV I L
Sbjct: 433 GIGDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 475

BLAST of Cp4.1LG03g03140 vs. ExPASy Swiss-Prot
Match: Q84KJ7 (Ammonium transporter 2 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-1 PE=2 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 1.6e-192
Identity = 336/479 (70.15%), Postives = 393/479 (82.05%), Query Frame = 0

Query: 15  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 74
           P WLN GDN+WQ+TASTLVG+QSMPGLV+LY SIVKKKWAVNSAFM LYA+A+ L+ WVL
Sbjct: 15  PDWLNKGDNAWQLTASTLVGIQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASSLLVWVL 74

Query: 75  VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY 134
           VG+RMAFGDQL+P WGK   ALTQSYL+ RA +P + +    G +  R EP  P A+LV 
Sbjct: 75  VGFRMAFGDQLLPFWGKAGVALTQSYLVGRATLPATAH----GAIP-RTEPFYPEATLVL 134

Query: 135 FQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYS 194
           FQF FAAIT++LLAGSVL RMNIKAWMAF PLW++ +YTVGA+S+WGGGFL++WGVIDYS
Sbjct: 135 FQFEFAAITLVLLAGSVLGRMNIKAWMAFTPLWLLLSYTVGAFSLWGGGFLYRWGVIDYS 194

Query: 195 GGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHS 254
           GGYVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLWMGW+GFNGGAP++
Sbjct: 195 GGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWMGWAGFNGGAPYA 254

Query: 255 ANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGVVQTW 314
           AN+ AS+AVLNTNV AATSLL+WT LDV++F KPSVIGA+QGMMTGL C+TPGAG+VQTW
Sbjct: 255 ANIAASVAVLNTNVCAATSLLMWTCLDVIFFRKPSVIGAVQGMMTGLVCITPGAGLVQTW 314

Query: 315 AAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLC 374
           AA++MGI  GS+PW +MM+LHKKS+ L K DDTL VFHTHAVAG++GG+LTGLLA P L 
Sbjct: 315 AAVVMGIFAGSVPWFTMMILHKKSALLMKVDDTLAVFHTHAVAGLLGGILTGLLATPELF 374

Query: 375 DLYLPITGTRGAFYGKNGGV-QFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEA 434
            L   + G RGAFYG  GG+ Q  KQL G+AF+I WN+V TT ILL I LF+PLRMPDE 
Sbjct: 375 SLESTVPGLRGAFYG--GGIKQIGKQLGGAAFVIAWNLVVTTAILLGIGLFIPLRMPDEQ 434

Query: 435 LMIGDDAVHGEEAYALWGDGEKFDPRRHGTA---GNNIEEGTASPYIH--GARGVIIEL 488
           LMIGDDA HGEEAYALWGDGEKFD  RH  +   G    +G A   +   GARGV I+L
Sbjct: 435 LMIGDDAAHGEEAYALWGDGEKFDATRHDLSRGGGGGDRDGPAGERLSALGARGVTIQL 486

BLAST of Cp4.1LG03g03140 vs. ExPASy Swiss-Prot
Match: Q8S233 (Ammonium transporter 2 member 3 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-3 PE=2 SV=1)

HSP 1 Score: 659.8 bits (1701), Expect = 2.3e-188
Identity = 325/481 (67.57%), Postives = 388/481 (80.67%), Query Frame = 0

Query: 15  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 74
           P WLNTGDN WQ+ A+T VGLQSMPGLV+LY SIVKKKWAVNSAFM LYA+A+ LI WVL
Sbjct: 18  PEWLNTGDNGWQLAAATFVGLQSMPGLVVLYGSIVKKKWAVNSAFMALYAYASTLIVWVL 77

Query: 75  VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRV-TLRIEPNIPMASLV 134
           VG+RMAFGD+L+P WGK   ALT+ +L+ RA++P + +   DG + + R EP  P AS+V
Sbjct: 78  VGFRMAFGDRLLPFWGKAGAALTEGFLVARASVPATAHYGKDGALESPRTEPFYPEASMV 137

Query: 135 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDY 194
            FQF  AAIT++LLAGS+L RMNIKAWMAF PLW++F+YTV A+S+WGGGFL+QWGVIDY
Sbjct: 138 LFQFELAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVCAFSLWGGGFLYQWGVIDY 197

Query: 195 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 254
           SGGYVIHLSSGIAG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 198 SGGYVIHLSSGIAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 257

Query: 255 SANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGVVQT 314
           + N+ ASIAVLNTNVSAA SLL WT LDV++FGKPSVIGA+QGMMTGL C+TPGAG+V T
Sbjct: 258 APNITASIAVLNTNVSAAASLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHT 317

Query: 315 WAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTL 374
           WAA++MGI GGS+PW SMM+LHK+S+ LQK DDTL VFHTHAVAG++GG LTGL A P L
Sbjct: 318 WAAILMGICGGSLPWFSMMILHKRSALLQKVDDTLAVFHTHAVAGLLGGFLTGLFALPDL 377

Query: 375 CDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEA 434
             ++  I G RGAFYG  G  Q  KQ+AG+ F++ WNVV+TT+ILL + L +PLRMPDE 
Sbjct: 378 TAVHTHIPGARGAFYG-GGIAQVGKQIAGALFVVVWNVVATTVILLGVGLVVPLRMPDEQ 437

Query: 435 LMIGDDAVHGEEAYALWGDGEKFDPRRH-----GTAGNNIEEGTASPYI--HGARGVIIE 488
           L IGDDA HGEEAYALWGDGE+FD  RH     G  G  + +      +   GARGV I+
Sbjct: 438 LKIGDDAAHGEEAYALWGDGERFDVTRHEGARGGAWGAAVVDEAMDHRLAGMGARGVTIQ 497

BLAST of Cp4.1LG03g03140 vs. ExPASy Swiss-Prot
Match: Q84KJ6 (Ammonium transporter 3 member 1 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT3-1 PE=2 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 6.8e-188
Identity = 321/474 (67.72%), Postives = 387/474 (81.65%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           M+   QP  MA   P WLN GDN+WQ+ ++TLVG+QS+PGLVILY SIVKKKWAVNSAFM
Sbjct: 8   MSVAYQPSGMAV--PEWLNKGDNAWQMISATLVGMQSVPGLVILYGSIVKKKWAVNSAFM 67

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPES-----GNTNN 120
            LYAFAAV +CWV  GY M+FG +L+P WGK  PAL QS+L+ +A +P++     G    
Sbjct: 68  ALYAFAAVWLCWVTWGYNMSFGHKLLPFWGKARPALGQSFLLAQAVLPQTTQFYKGGGGA 127

Query: 121 DGRV-TLRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTV 180
           D  V T  + P  PMA++VYFQ  FAAIT+ILLAGS+L RMNIKAWM FVPLW+ F+YTV
Sbjct: 128 DAVVETPWVNPLYPMATMVYFQCVFAAITLILLAGSLLGRMNIKAWMLFVPLWLTFSYTV 187

Query: 181 GAYSVWGGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLML 240
           GA+S+WGGGFLF WGV+DYSGGYVIHLSSG+AG TAAYWVGPR   DRERFPPNNVLLML
Sbjct: 188 GAFSLWGGGFLFHWGVMDYSGGYVIHLSSGVAGFTAAYWVGPRSTKDRERFPPNNVLLML 247

Query: 241 AGAGLLWMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAI 300
            GAG+LWMGW+GFNGG P+SAN+ +S+AVLNTN+ AATSLLVWT LDV++F KPSVIGA+
Sbjct: 248 TGAGILWMGWAGFNGGDPYSANIDSSLAVLNTNICAATSLLVWTCLDVIFFKKPSVIGAV 307

Query: 301 QGMMTGLACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTH 360
           QGM+TGL C+TPGAG+VQ WAA++MGIL GSIPW +MMV+HK+S  LQ+ DDTLGVFHTH
Sbjct: 308 QGMITGLVCITPGAGLVQGWAAIVMGILSGSIPWFTMMVVHKRSRLLQQVDDTLGVFHTH 367

Query: 361 AVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFY-GKNGGVQFLKQLAGSAFIIGWNVVS 420
           AVAG +GG  TGL AEP LC L+LP+T +RGAFY G+ GG+QF++Q+AG+ FII WNVV 
Sbjct: 368 AVAGFLGGATTGLFAEPVLCSLFLPVTNSRGAFYPGRGGGLQFVRQVAGALFIICWNVVV 427

Query: 421 TTIILLFIRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN 468
           T+++ L +R  +PLRMP+E L IGDDAVHGEEAYALWGDGEK+D  +HG   +N
Sbjct: 428 TSLVCLAVRAVVPLRMPEEELAIGDDAVHGEEAYALWGDGEKYDSTKHGWYSDN 479

BLAST of Cp4.1LG03g03140 vs. ExPASy Swiss-Prot
Match: Q8S230 (Ammonium transporter 2 member 2 OS=Oryza sativa subsp. japonica OX=39947 GN=AMT2-2 PE=2 SV=2)

HSP 1 Score: 656.8 bits (1693), Expect = 2.0e-187
Identity = 323/481 (67.15%), Postives = 385/481 (80.04%), Query Frame = 0

Query: 15  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 74
           P WLN GD +WQ+ A+T VG+QSMPGLV++Y SIVKKKWAVNSAFM LYA+A+ LI WVL
Sbjct: 22  PAWLNKGDTAWQLVAATFVGIQSMPGLVVIYGSIVKKKWAVNSAFMALYAYASTLIVWVL 81

Query: 75  VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRV-TLRIEPNIPMASLV 134
           VG+RMAFGD+L+P W K  PALTQ +L+ RA  P + +  +DG + T R EP    A+LV
Sbjct: 82  VGFRMAFGDRLLPFWAKAGPALTQDFLVQRAVFPATAHYGSDGTLETPRTEPFYAEAALV 141

Query: 135 YFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDY 194
            F+F FAAIT++LLAGS+L RMNIKAWMAF PLW++F+YTVGA+S+WGGGFL+QWGVIDY
Sbjct: 142 LFEFEFAAITLVLLAGSLLGRMNIKAWMAFTPLWLLFSYTVGAFSLWGGGFLYQWGVIDY 201

Query: 195 SGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPH 254
           SGGYVIHLSSG+AG TAAYWVGPR+KSDRERF PNN+LLM+AG GLLW+GW+GFNGGAP+
Sbjct: 202 SGGYVIHLSSGVAGFTAAYWVGPRLKSDRERFSPNNILLMIAGGGLLWLGWAGFNGGAPY 261

Query: 255 SANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGVVQT 314
           + NV A++AVLNTNVSAATSLL WT LDV++FGKPSVIGA+QGMMTGL C+TPGAG+V T
Sbjct: 262 APNVTATVAVLNTNVSAATSLLTWTCLDVIFFGKPSVIGAVQGMMTGLVCITPGAGLVHT 321

Query: 315 WAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTL 374
           W+AM+MG+  GS+PW +MM+LHKKS+FL K DDTL VFHTHAVAG++GG+LTGLLA P L
Sbjct: 322 WSAMLMGMFAGSVPWFTMMILHKKSTFLMKVDDTLAVFHTHAVAGILGGVLTGLLATPEL 381

Query: 375 CDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEA 434
           C L  PI   RG FYG   G Q  KQL G+ F+  WN++ T+ ILL I LF+PLRM D+ 
Sbjct: 382 CALDCPIPNMRGVFYGSGIG-QLGKQLGGALFVTVWNLIVTSAILLCIGLFIPLRMSDDQ 441

Query: 435 LMIGDDAVHGEEAYALWGDGEKFD-----PRRHGTAGNNIEEGTASPYI--HGARGVIIE 488
           LMIGDDA HGEEAYALWGDGEKFD       R G AG    E T    +   GARGV I+
Sbjct: 442 LMIGDDAAHGEEAYALWGDGEKFDVTRPETTRTGGAGGAGREDTMEQRLTNMGARGVTIQ 501

BLAST of Cp4.1LG03g03140 vs. NCBI nr
Match: XP_023529182.1 (ammonium transporter 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 969 bits (2504), Expect = 0.0
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM
Sbjct: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA
Sbjct: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480

Query: 481 RGVIIEL 487
           RGVIIEL
Sbjct: 481 RGVIIEL 487

BLAST of Cp4.1LG03g03140 vs. NCBI nr
Match: XP_022982616.1 (ammonium transporter 2-like [Cucurbita maxima])

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 483/487 (99.18%), Postives = 486/487 (99.79%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRA+IPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRADIPESGNTNNDGRVT 120

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKK SFLQKADDTLGVFHTHAVAG+M
Sbjct: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGLM 360

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWN+VSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNIVSTTIILLF 420

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA
Sbjct: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480

Query: 481 RGVIIEL 487
           RGVIIEL
Sbjct: 481 RGVIIEL 487

BLAST of Cp4.1LG03g03140 vs. NCBI nr
Match: XP_022934329.1 (ammonium transporter 2-like [Cucurbita moschata] >KAG6580457.1 Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 482/487 (98.97%), Postives = 484/487 (99.38%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAG+VQTWAAMIMGILGGSIPWVSMMVLHKK SFLQKADDTLGVFHTHAVAGMM
Sbjct: 301 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 360

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN+EEG ASPYIHGA
Sbjct: 421 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 480

Query: 481 RGVIIEL 487
           RGVIIEL
Sbjct: 481 RGVIIEL 487

BLAST of Cp4.1LG03g03140 vs. NCBI nr
Match: KAG7017209.1 (Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 482/487 (98.97%), Postives = 484/487 (99.38%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 85  MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 144

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT
Sbjct: 145 VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 204

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 205 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 264

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 265 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 324

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 325 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 384

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAG+VQTWAAMIMGILGGSIPWVSMMVLHKK SFLQKADDTLGVFHTHAVAGMM
Sbjct: 385 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 444

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF
Sbjct: 445 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 504

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN+EEG ASPYIHGA
Sbjct: 505 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 564

Query: 481 RGVIIEL 487
           RGVIIEL
Sbjct: 565 RGVIIEL 571

BLAST of Cp4.1LG03g03140 vs. NCBI nr
Match: KAG7027883.1 (Ammonium transporter 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 445/487 (91.38%), Postives = 468/487 (96.10%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTA P +MA G+PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 9   MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 68

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL QSYLIDRANIPESG+ ++DGRVT
Sbjct: 69  ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRVT 128

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
            RIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVGAY++W
Sbjct: 129 PRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTIW 188

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGG+VIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+L
Sbjct: 189 GGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGML 248

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTG
Sbjct: 249 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTG 308

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAGVVQTWAA+IMG++GGSIPWVSMM+LHKK SFLQKADDTLGVFHTHAVAGMM
Sbjct: 309 LACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQKADDTLGVFHTHAVAGMM 368

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLC+LYLPI  +RGAFYGKNGGVQFLKQLAG+ FIIGWN+VSTTIILLF
Sbjct: 369 GGLLTGLLAEPTLCELYLPIPDSRGAFYGKNGGVQFLKQLAGAMFIIGWNIVSTTIILLF 428

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEGT+ PYI+GA
Sbjct: 429 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANVEEGTSPPYINGA 488

Query: 481 RGVIIEL 487
           RGV I L
Sbjct: 489 RGVTITL 495

BLAST of Cp4.1LG03g03140 vs. ExPASy TrEMBL
Match: A0A6J1IZU0 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111481439 PE=3 SV=1)

HSP 1 Score: 963 bits (2489), Expect = 0.0
Identity = 483/487 (99.18%), Postives = 486/487 (99.79%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRA+IPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRADIPESGNTNNDGRVT 120

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKK SFLQKADDTLGVFHTHAVAG+M
Sbjct: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGLM 360

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWN+VSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNIVSTTIILLF 420

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA
Sbjct: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480

Query: 481 RGVIIEL 487
           RGVIIEL
Sbjct: 481 RGVIIEL 487

BLAST of Cp4.1LG03g03140 vs. ExPASy TrEMBL
Match: A0A6J1F297 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111441525 PE=3 SV=1)

HSP 1 Score: 961 bits (2485), Expect = 0.0
Identity = 482/487 (98.97%), Postives = 484/487 (99.38%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
           VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT
Sbjct: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
           LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW
Sbjct: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL
Sbjct: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG
Sbjct: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAG+VQTWAAMIMGILGGSIPWVSMMVLHKK SFLQKADDTLGVFHTHAVAGMM
Sbjct: 301 LACVTPGAGIVQTWAAMIMGILGGSIPWVSMMVLHKKLSFLQKADDTLGVFHTHAVAGMM 360

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF
Sbjct: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNN+EEG ASPYIHGA
Sbjct: 421 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNVEEGRASPYIHGA 480

Query: 481 RGVIIEL 487
           RGVIIEL
Sbjct: 481 RGVIIEL 487

BLAST of Cp4.1LG03g03140 vs. ExPASy TrEMBL
Match: A0A6J1GGC9 (Ammonium transporter OS=Cucurbita moschata OX=3662 GN=LOC111453662 PE=3 SV=1)

HSP 1 Score: 897 bits (2317), Expect = 0.0
Identity = 443/487 (90.97%), Postives = 467/487 (95.89%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTA P +MA G+PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVDMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL QSYLIDRANIPESG+ ++DGRVT
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLIPLWGKGIPALEQSYLIDRANIPESGHVSHDGRVT 165

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
            RIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVGAY++W
Sbjct: 166 PRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGAYTIW 225

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGG+VIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+L
Sbjct: 226 GGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGML 285

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTG
Sbjct: 286 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTG 345

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAGVVQTWAA+IMG++GGSIPWVSMM+LHK+ SFLQKADDTLGVFHTHAVAGMM
Sbjct: 346 LACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKRLSFLQKADDTLGVFHTHAVAGMM 405

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLC+LYLPI  +RGAFYGKNGGVQFLKQL G+ FIIGWN+VSTTIILLF
Sbjct: 406 GGLLTGLLAEPTLCELYLPIPDSRGAFYGKNGGVQFLKQLTGAMFIIGWNIVSTTIILLF 465

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEGT+ PYI+GA
Sbjct: 466 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANVEEGTSPPYINGA 525

Query: 481 RGVIIEL 487
           RGV I L
Sbjct: 526 RGVTITL 532

BLAST of Cp4.1LG03g03140 vs. ExPASy TrEMBL
Match: A0A6J1I2C9 (Ammonium transporter OS=Cucurbita maxima OX=3661 GN=LOC111470255 PE=3 SV=1)

HSP 1 Score: 895 bits (2313), Expect = 0.0
Identity = 443/487 (90.97%), Postives = 466/487 (95.69%), Query Frame = 0

Query: 1   MNNTAQPGNMAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 60
           MNNTA P +MA G+PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM
Sbjct: 46  MNNTAPPVHMATGTPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM 105

Query: 61  VLYAFAAVLICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVT 120
            LYAFAAVLICWVLVGYRMAFGDQL+PLWGKGIPAL QSYLIDRANIPESG+ ++DGRVT
Sbjct: 106 ALYAFAAVLICWVLVGYRMAFGDQLLPLWGKGIPALGQSYLIDRANIPESGHVSHDGRVT 165

Query: 121 LRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVW 180
            RIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIF+YTVG Y++W
Sbjct: 166 PRIEPNIPMASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFSYTVGTYTIW 225

Query: 181 GGGFLFQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLL 240
           GGGFLFQWGVIDYSGG+VIHLSSGI+GLTAAYWVGPRIKSDRERFPPNNVLLMLAGAG+L
Sbjct: 226 GGGFLFQWGVIDYSGGFVIHLSSGISGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGML 285

Query: 241 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTG 300
           WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTG
Sbjct: 286 WMGWSGFNGGAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTG 345

Query: 301 LACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMM 360
           LACVTPGAGVVQTWAA+IMG++GGSIPWVSMM+LHKK SFLQK DDTLGVFHTHAVAGMM
Sbjct: 346 LACVTPGAGVVQTWAAIIMGVMGGSIPWVSMMILHKKLSFLQKVDDTLGVFHTHAVAGMM 405

Query: 361 GGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLF 420
           GGLLTGLLAEPTLC+LYLPI  +RGAFYGKNGGVQFLKQLAG+ FIIGWN+VSTTIILLF
Sbjct: 406 GGLLTGLLAEPTLCELYLPIPDSRGAFYGKNGGVQFLKQLAGAMFIIGWNIVSTTIILLF 465

Query: 421 IRLFMPLRMPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGA 480
           IRLFMPLRMPDE LMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEGT+ PYI+GA
Sbjct: 466 IRLFMPLRMPDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANVEEGTSPPYINGA 525

Query: 481 RGVIIEL 487
           RGV I L
Sbjct: 526 RGVTIPL 532

BLAST of Cp4.1LG03g03140 vs. ExPASy TrEMBL
Match: A0A0A0LCQ1 (Ammonium transporter OS=Cucumis sativus OX=3659 GN=Csa_3G730930 PE=3 SV=1)

HSP 1 Score: 879 bits (2272), Expect = 0.0
Identity = 441/478 (92.26%), Postives = 456/478 (95.40%), Query Frame = 0

Query: 10  MAAGSPPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVL 69
           MAAGSP WLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL
Sbjct: 1   MAAGSPSWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVL 60

Query: 70  ICWVLVGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPM 129
           ICWV+VGYRMAFGDQL+PLWGKGIPAL+QSYLI + NIPESG   NDGR T RI+PN+PM
Sbjct: 61  ICWVVVGYRMAFGDQLIPLWGKGIPALSQSYLIGQGNIPESGV--NDGRGTPRIQPNVPM 120

Query: 130 ASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWG 189
           ASLVYFQFTFAAITVILL GSVLARMNIKAWMAFVPLWVIF+YTVGAYSVWGGGFLFQWG
Sbjct: 121 ASLVYFQFTFAAITVILLGGSVLARMNIKAWMAFVPLWVIFSYTVGAYSVWGGGFLFQWG 180

Query: 190 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 249
           VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG
Sbjct: 181 VIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNG 240

Query: 250 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAG 309
           GAPHSANVVASIAVLNTNVSAATSLLVWTILDV YFGKPSVIGAIQGMMTGLACVTPGAG
Sbjct: 241 GAPHSANVVASIAVLNTNVSAATSLLVWTILDVFYFGKPSVIGAIQGMMTGLACVTPGAG 300

Query: 310 VVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMMGGLLTGLLA 369
           VVQTWAA+IMGIL GSIPW+SMMVLHKK SFLQKADDTLGVFHTHAVAG+MGGLLTGLLA
Sbjct: 301 VVQTWAAIIMGILAGSIPWLSMMVLHKKLSFLQKADDTLGVFHTHAVAGLMGGLLTGLLA 360

Query: 370 EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRM 429
           EPTLCDLYLPI GTRGAFYG +GGVQFLKQLAGS FIIGWN+VSTT+ILLFIRLFMPLRM
Sbjct: 361 EPTLCDLYLPIIGTRGAFYGGSGGVQFLKQLAGSTFIIGWNIVSTTVILLFIRLFMPLRM 420

Query: 430 PDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGARGVIIEL 487
           PDE LMIGDDAVHGEEAYALWGDGEKFDPRRHG A  N+EEG  SPYI+GARGVIIEL
Sbjct: 421 PDEELMIGDDAVHGEEAYALWGDGEKFDPRRHGNAAANMEEGAGSPYINGARGVIIEL 476

BLAST of Cp4.1LG03g03140 vs. TAIR 10
Match: AT2G38290.1 (ammonium transporter 2 )

HSP 1 Score: 709.1 bits (1829), Expect = 2.4e-204
Identity = 349/473 (73.78%), Postives = 397/473 (83.93%), Query Frame = 0

Query: 15  PPWLNTGDNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVL 74
           P WLN GDN+WQ+TA+TLVGLQSMPGLVILYASIVKKKWAVNSAFM LYAFAAVL+CWVL
Sbjct: 13  PEWLNKGDNAWQLTAATLVGLQSMPGLVILYASIVKKKWAVNSAFMALYAFAAVLLCWVL 72

Query: 75  VGYRMAFGDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY 134
           + Y+MAFG++L+P WGKG PA  Q YL  +A IP S              P  PMA+LVY
Sbjct: 73  LCYKMAFGEELLPFWGKGGPAFDQGYLKGQAKIPNSNVA----------APYFPMATLVY 132

Query: 135 FQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQWGVIDYS 194
           FQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IF+YTVGAYS+WGGGFL+QWGVIDYS
Sbjct: 133 FQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQWGVIDYS 192

Query: 195 GGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPHS 254
           GGYVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFNGGAP++
Sbjct: 193 GGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFNGGAPYA 252

Query: 255 ANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGAGVVQTW 314
           AN+ +SIAVLNTN+SAATSLLVWT LDV++FGKPSVIGAIQGM+TGLA VTPGAG++QTW
Sbjct: 253 ANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGAGLIQTW 312

Query: 315 AAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMMGGLLTGLLAEPTLC 374
           AA+I+G++ G+ PW SMM++HKKS+ LQK DDTL VF+THAVAG++GG++TGL A P LC
Sbjct: 313 AAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLFAHPDLC 372

Query: 375 DLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLRMPDEAL 434
            L LP+  TRGAFYG NGG Q LKQLAG+AFI  WNVVSTTIILL IR+F+PLRM +E L
Sbjct: 373 VLVLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLRMAEEEL 432

Query: 435 MIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGARGVIIEL 488
            IGDDA HGEEAYALWGDGEKFD  RH       +E     Y+HGARGV I L
Sbjct: 433 GIGDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 475

BLAST of Cp4.1LG03g03140 vs. TAIR 10
Match: AT2G38290.2 (ammonium transporter 2 )

HSP 1 Score: 564.3 bits (1453), Expect = 9.5e-161
Identity = 274/359 (76.32%), Postives = 312/359 (86.91%), Query Frame = 0

Query: 129 MASLVYFQFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWGGGFLFQW 188
           MA+LVYFQFTFAAIT IL+AGSVL RMNIKAWMAFVPLW+IF+YTVGAYS+WGGGFL+QW
Sbjct: 1   MATLVYFQFTFAAITTILVAGSVLGRMNIKAWMAFVPLWLIFSYTVGAYSIWGGGFLYQW 60

Query: 189 GVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPPNNVLLMLAGAGLLWMGWSGFN 248
           GVIDYSGGYVIHLSSG+AG  AAYWVGPR K+DRERFPPNNVLLMLAGAGLLWMGWSGFN
Sbjct: 61  GVIDYSGGYVIHLSSGVAGFVAAYWVGPRPKADRERFPPNNVLLMLAGAGLLWMGWSGFN 120

Query: 249 GGAPHSANVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVIGAIQGMMTGLACVTPGA 308
           GGAP++AN+ +SIAVLNTN+SAATSLLVWT LDV++FGKPSVIGAIQGM+TGLA VTPGA
Sbjct: 121 GGAPYAANLTSSIAVLNTNLSAATSLLVWTTLDVIFFGKPSVIGAIQGMVTGLAGVTPGA 180

Query: 309 GVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVFHTHAVAGMMGGLLTGLL 368
           G++QTWAA+I+G++ G+ PW SMM++HKKS+ LQK DDTL VF+THAVAG++GG++TGL 
Sbjct: 181 GLIQTWAAIIIGVVSGTAPWASMMIIHKKSALLQKVDDTLAVFYTHAVAGLLGGIMTGLF 240

Query: 369 AEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNVVSTTIILLFIRLFMPLR 428
           A P LC L LP+  TRGAFYG NGG Q LKQLAG+AFI  WNVVSTTIILL IR+F+PLR
Sbjct: 241 AHPDLCVLVLPLPATRGAFYGGNGGKQLLKQLAGAAFIAVWNVVSTTIILLAIRVFIPLR 300

Query: 429 MPDEALMIGDDAVHGEEAYALWGDGEKFDPRRHGTAGNNIEEGTASPYIHGARGVIIEL 488
           M +E L IGDDA HGEEAYALWGDGEKFD  RH       +E     Y+HGARGV I L
Sbjct: 301 MAEEELGIGDDAAHGEEAYALWGDGEKFDATRHVQQFERDQEAAHPSYVHGARGVTIVL 359

BLAST of Cp4.1LG03g03140 vs. TAIR 10
Match: AT1G64780.1 (ammonium transporter 1;2 )

HSP 1 Score: 102.4 bits (254), Expect = 1.0e-21
Identity = 126/484 (26.03%), Postives = 191/484 (39.46%), Query Frame = 0

Query: 22  DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAF 81
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  I + L G+  AF
Sbjct: 52  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGAISYYLFGFAFAF 111

Query: 82  GDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYFQFTFAA 141
           G         G      S+    ++ PE   ++                S   +Q+ FA 
Sbjct: 112 GTP-----SNGFIGRHHSFFA-LSSYPERPGSD---------------FSFFLYQWAFAI 171

Query: 142 ITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWG-----------GGFLFQWGV 201
               + +GS+  R    A++ +      F Y   ++  W               LF  G 
Sbjct: 172 AAAGITSGSIAERTQFVAYLIYSTFLTGFVYPTVSHWFWSSDGWASASRSDNNLLFGSGA 231

Query: 202 IDYSGGYVIHLSSGIAGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLLWMGWS 261
           ID++G  V+H+  GIAGL  A   GPRI + DR         ++  L++ G  LLW GW 
Sbjct: 232 IDFAGSGVVHMVGGIAGLCGALVEGPRIGRFDRSGRSVALRGHSASLVVLGTFLLWFGWY 291

Query: 262 GFNGGA------------PHSA--NVVASIAVLNTNVSAATSLLVWTILDVLYFGKPSVI 321
           GFN G+            P+    + V   AV  T +S  T+ L       L  G  +VI
Sbjct: 292 GFNPGSFLTILKGYDKSRPYYGQWSAVGRTAV-TTTLSGCTAALTTLFSKRLLAGHWNVI 351

Query: 322 GAIQGMMTGLACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLGVF 381
               G++ G A +T G  VV+ WAA++ G +   +  +   +L KK     K DD L   
Sbjct: 352 DVCNGLLGGFAAITSGCAVVEPWAAIVCGFVASWV-LIGFNLLAKK----LKYDDPLEAA 411

Query: 382 HTHAVAGMMGGLLTGLLAEPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIGWNV 441
             H   G  G + TGL A     +         G F G  GG     Q+     I+GW  
Sbjct: 412 QLHGGCGAWGLIFTGLFARKEYVNEIYSGDRPYGLFMG-GGGKLLAAQIVQIIVIVGWVT 471

Query: 442 VSTTIILLFIRLFMPLRMPDEALMIGDDAV-HGEEAYALWGDGEKFDPRRHGTAGNNIEE 475
           V+   +   +     LR+  E  M G D   HG  AYA + D +    +  G     +E 
Sbjct: 472 VTMGPLFYGLHKMNLLRISAEDEMAGMDMTRHGGFAYA-YNDEDDVSTKPWGHFAGRVEP 505

BLAST of Cp4.1LG03g03140 vs. TAIR 10
Match: AT3G24290.1 (ammonium transporter 1;5 )

HSP 1 Score: 102.1 bits (253), Expect = 1.3e-21
Identity = 121/466 (25.97%), Postives = 186/466 (39.91%), Query Frame = 0

Query: 22  DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAF 81
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  + + L GY  AF
Sbjct: 46  DNTYLLFSAYLVFAMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 105

Query: 82  GDQLVPLWGK------GIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVYF 141
           G+      G+        P LT  Y                              S   +
Sbjct: 106 GESSDGFIGRHNFGLQNFPTLTSDY------------------------------SFFLY 165

Query: 142 QFTFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWG-GGF---------L 201
           Q+ FA     + +GS+  R    A++ +      F Y V ++  W   G+         L
Sbjct: 166 QWAFAIAAAGITSGSIAERTKFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSEDRL 225

Query: 202 FQWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRIKSDRERFPP---------NNVLLMLAG 261
           F  G ID++G  V+H+  GIAGL  A   GPRI     RFP          ++  L++ G
Sbjct: 226 FGTGAIDFAGSGVVHMVGGIAGLWGALIEGPRI----GRFPDGGHAIALRGHSASLVVLG 285

Query: 262 AGLLWMGWSGFNGGA------PHSA-------NVVASIAVLNTNVSAATSLLVWTILDVL 321
             LLW GW GFN G+      P+++       + +   AV  T +S  T+ L       L
Sbjct: 286 TFLLWFGWYGFNPGSFTKILIPYNSGSNYGQWSGIGRTAV-TTTLSGCTAALTTLFGKRL 345

Query: 322 YFGKPSVIGAIQGMMTGLACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQK 381
             G  +V     G++ G A +T G  VV  WAA++ G +   +     ++   K + L K
Sbjct: 346 LSGHWNVTDVCNGLLGGFAAITAGCSVVDPWAAIVCGFVASLV-----LIGCNKLAELLK 405

Query: 382 ADDTLGVFHTHAVAGMMGGLLTGLLA-EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAG 441
            DD L     H   G  G +  GL A E  + ++Y    G     +   GG     QL  
Sbjct: 406 YDDPLEAAQLHGGCGAWGLIFVGLFAKEKYINEVYGASPGRHYGLFMGGGGKLLGAQLVQ 465

Query: 442 SAFIIGWNVVSTTIILLFIRLFMPLRMPDEALMIGDD-AVHGEEAY 448
              I+GW   +   +   ++    LR+ ++  M G D A HG  AY
Sbjct: 466 IIVIVGWVSATMGTLFFILKKLNLLRISEQHEMRGMDLAGHGGFAY 470

BLAST of Cp4.1LG03g03140 vs. TAIR 10
Match: AT4G13510.1 (ammonium transporter 1;1 )

HSP 1 Score: 101.3 bits (251), Expect = 2.3e-21
Identity = 129/488 (26.43%), Postives = 189/488 (38.73%), Query Frame = 0

Query: 22  DNSWQITASTLVGLQSMPGLVILYASIVKKKWAVNSAFMVLYAFAAVLICWVLVGYRMAF 81
           DN++ + ++ LV    + G  +L A  V+ K  +N     +   AA  + + L GY  AF
Sbjct: 42  DNTYLLFSAYLVFSMQL-GFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAF 101

Query: 82  GDQLVPLWGKGIPALTQSYLIDRANIPESGNTNNDGRVTLRIEPNIPMASLVY----FQF 141
           G       GK    L                             +IP AS  Y    +Q+
Sbjct: 102 GSPSNGFIGKHYFGL----------------------------KDIPTASADYSNFLYQW 161

Query: 142 TFAAITVILLAGSVLARMNIKAWMAFVPLWVIFAYTVGAYSVWG-----------GGFLF 201
            FA     + +GS+  R    A++ +      F Y V ++  W            G  LF
Sbjct: 162 AFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLF 221

Query: 202 QWGVIDYSGGYVIHLSSGIAGLTAAYWVGPRI-KSDRE----RFPPNNVLLMLAGAGLLW 261
             G ID++G  V+H+  GIAGL  A   GPR+ + D          ++  L++ G  LLW
Sbjct: 222 STGAIDFAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLW 281

Query: 262 MGWSGFNGGAPHSANVVASIAVLNTNVSA------ATSLLVWTILDVLYFGKP------S 321
            GW GFN G+ +   V       N   SA       T+L   T      FGK       +
Sbjct: 282 FGWYGFNPGSFNKILVTYETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWN 341

Query: 322 VIGAIQGMMTGLACVTPGAGVVQTWAAMIMGILGGSIPWVSMMVLHKKSSFLQKADDTLG 381
           V     G++ G A +T G  VV+ WAA+I G +   +    ++  +K +  L K DD L 
Sbjct: 342 VTDVCNGLLGGFAAITGGCSVVEPWAAIICGFVAALV----LLGCNKLAEKL-KYDDPLE 401

Query: 382 VFHTHAVAGMMGGLLTGLLA-EPTLCDLYLPITGTRGAFYGKNGGVQFLKQLAGSAFIIG 441
               H   G  G + T L A E  L  +Y    G     +   GG     QL     I G
Sbjct: 402 AAQLHGGCGAWGLIFTALFAQEKYLNQIYGNKPGRPHGLFMGGGGKLLGAQLIQIIVITG 461

Query: 442 WNVVSTTIILLFIRLFMPLRMPDEALMIGDDAV-HGEEAYALWGDGE--------KFDPR 468
           W   +   +   ++    LR+  E  M G D   HG  AY  + D E        + +PR
Sbjct: 462 WVSATMGTLFFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDESHKAIQLRRVEPR 495