Cp4.1LG03g02840 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g02840
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Ubiquitin thioesterase OTU1
Location	Cp4.1LG03: 391188 .. 395133 (+)
RNA-Seq Expression	Cp4.1LG03g02840
Synteny	Cp4.1LG03g02840

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGGGCTCAGGTTCTGGCTGATTTGTTCATTACTTTTTTAGGGTACAATTATTCCTTCATTGATTTATATATTAAATGAGCATTCATTTTCTCCAATTAATTTTGTACCAAAATTTAGTTATAGAAATTAGAATAGGATCTTCATTTCTGCTTTCTCCAGCCATAGGCATAGCCAGAGCCATGGACTTTAATGAGTAGGGAGGGTGGCTTGGCTATAAGGTTCCAGAGGCTTCGTTTTCTGTGAAAGTTTCTTTCGGCATGTACATACAAACTAAAGTGGGGCCATAGTACAAATCTGAATCAAACCCACAACCAGATTCCTCGAACCCATCACACTAATGGCTTCGGACCAGACTCTGTTCCACAACAGCCTTCTCGCCTTGTACCTCATCGGACCACCGACCGCCGTCTCCCTGAGGTTTCTCCAGGCGCCCTACGGGAAGCACAACCGCCCGGGGTGGGGCCCGACCATGTCGGTTCCGTTGGCGTGGTTCCTCATGGAAAGTCCCACCCTATGGCTGACTCTCCTACTCTTCCCCTCCGGTGATAACTCCTCCAACTACAAGTCTCTCCTTCTCATCTCCCTCTTCCTCCTCCACTATCTCAATCGCACCTGCCTCTACCCAATTCGCCTTCTCCGGAGTGGGACTCGACGGAATTCCGCCACCGGTTTCCCGGTGGTTGTGGCGCTCATGGCATTCGGGTTCAATTTGCTCAACTCTTACCTGCAGACCAGATCTGTGTCTCACTACGTGGATTACGACAGAGACGAGTGGTTTTGGTGGCGGTTTTTCGGTGGGGCGGTGGTTTTCGTGCTGGGGATGGCAGTGAATATTTGGTCGGATGGTGTTCTGGTAAAATTGAAGATGGCGGGCGGCGGATATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTATTTGGGAGAGATTGTGGAGTGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTACTTGAACAAGTTCGGGGAGGATTATCCCCGAGATAGGAAGGCCGTTATTCCCTTCCTGTATTAAGTGTCTGTATCAAAAATTAAAATTAATAAATAATCTTAAATTCTCTTATTTTAATTGATTAAAAAAAAAAATGAAAATTATTTATATTTAAAGAAATGGATGAAATTGTATTGTAACATAGTTTTGCCGGGCAGCGCCTCCCTCTTCGTAGCTTGCTTCTTTTCCATGGATATTATGGCGGAGAAACCTCAAAATGGTGAGACCTTCTTCATTCTTATGGCTCCTCTTCTCATTGTCTCTCTGTTTTTCTTCGTTATTTTTCCTTTTCGAGTTATTGTCGGTTTGTTCATCTTGTAGTGGCGATTCTCGAGCAATTGCGACTAGGAATTGCTCGATTTGAGCTCGTATCTTCGCCTGTAAATTCAATTTCACCTTCCAACTCAGTCGCTCCTTCTTTCCCTTTTTCTATGAATCGCGGCCGCCCACTCTTTGCTAAAATTGGACCCATGCTGTAAGTTCATTCATTCTCTTTTAGTTGGGCGAAAGTTTTTGATATCCGTGTCTTTTCGGAATTCATAAATTGTTGTTCTTAGGCGCGCTGAATCGCCTGCATTGAGGAAAGTTGAGCACTACAAAGTCCAGAAAGTTACTGGAGATGGACGCTGCCTATTCCGTGCGCTTGTAACCTATCTTCCCTTATTCTTTTCTTTTGTTCTCTCTTGAATTTATTTTCTGATTCCCTCTGTTTATTCTAATTTATGTTTCAAATTATCTAATTGTGGATTTTTGTTTTTCACTTTCTTTGCTATGCTTTTGATGCCACTTCATTTTCGGTCTAAAAACCAAATTTTAAAAACAAAAGTTATGAAGTGAAACACAAATGGATTTTTAGAAACAAGGATAATCTCCAGAAATAAGATTAAGGACAGTGATTCGCAAGTATTTGTATTCCTGCTTACTGGCTGTCTCAAGGAAGTGGGAGCAATCTTTATTGCCTTTTAAACTAACTTCCTCTTAATAAAATCAGGCCAAAGGAATGGCTTTCAACAAGGGGATTCCTCTTCGTCCATTTGAGGAGAAAAATGATGCAGGTAAGCTCTGATATATCATCTCTTATAGGCACAATGCGAGTGCAGACATATCTTCTTGGATAATTGAATGCGGAACATAATCTTCATGTTGATTGAACAAGTAGGAAGATTGTGCAAATATTCTGTTTACTATATCAACCAAATGTTTTTGGGTGCTCCAGTTGTCTACTAAGGTTACCATGAACATCAAAGCTAGAGTCAATTTTTTTTTTGTCTTGAATTAGTGTTTCATTTTGACAAGCCCACTTCGACACTCAGGTGACCTTGACGAGTGAGTTGTGCTTGTAGCACAAAAATATGTTTATCAGTCACACAACAAAAGGCGTATGGGACTGTGGACATCTCTCTTTTTTCTAACTGTGAGATTCCACCTCGGTTGGCAAGGAGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCTTAGCATACGCGTTTTAAAAACCTTTAAGGGAAGTCTGAAAGGGAAAGCCCAAAGAGGACAATATCTTCTGGGCGGTGGGCTTGGTCTGTTACACTAATAGTTTTCAAGTGTTCGTAATCATATGAGTATACATTCCGGAGTTTGTGTATGAATGATCTAAAGTTCCAATTTTCATAAATCATTGATGTTGGTAGCCTAAACATGCAGATGATTTACGCATGGCTGTGAAAGAAATTATCTGTGATAATGGCAAAGAAAGACGGCAGTATGAAGAAGCTCTCATTGCAATTACTGCTGAGGAGCCATTAGAACGGTTGGTTGGTTTCCCTTTTTACTTTTTTATTCTTTCTATTCATGTTAAGTTGTCCTCTTAGATTTTGGATTTCCACGTCTTTGATTGCTGTCTGATAATGAGAACTTCCAAAAATAAATTCAGCTACTGCCGACGAATTCGTACACCTAATTTCTGGGGAGGAGAGTCAGAGCTATTGGTTAGTTCTCTTCTTCTGGCGTATCTTCACAAGCTATACTCAGATTTTTGTTAATCTATTTTGCATTTACGCGCCATGTGTAAAGCCACTATTTGTTCACGTCACGTATGAATCATGATCTGTATTAACTTCTATCAGGTATTGTCAAAGCTCTGCAAGCAACCTATCATTGTCTACATACCAGAGCATGAGGTATACTTTTCTCAACTAAGATACCTAAATCAATACCCATACAAATGTTGGTGTCTAAACTGAGGTCGAGTATATTCTCTGATAGTCTCGGGCAATTTCTGAGATAGGAACAGGATTAGGTGGTGTTACTCCCTTTAAGTTTAACTTCACAAACTTAATTCAGCCATCTCTGATAACGATTTCATTTGTGTCGACGTTTGAAAAGAATAGTAGAAATTAACATGTTCTCTACTTAATTTTCAGCACAGAATGGGTTCGCGTAGCTCTAGCTTTATTCCTATTGCAGAATATGGAGCTGAGTTCAAGGGAGGAAACCCCAAGAAACCTGTGAGGTTGCTTTACAGCGGTAGGAACCACTACGATCTGCTTGTTTGAGGAGGGGTGATGTGACCTTGTTGCCATCTAAAGATGAGCCAACGAAGACATGCTCGACCAACAATGAATGGTCGGAGGAACCCAATAACACAACACCCAAAGCTCTCTTATTGGTCAACAATTCCAAACTTTTGAGTTGTTTAAACATTGTTTTTTTATGAAGAAATAAATGAATTGATGACACTTTAAGAGAAATAGGACATAAAGGTTGGATTTTTGTTGAAATTTGTTGCTTTTGGGAGATTTGTTTAATGCTCTATCTTTCACGATAGCAAGCAATATTTTATTTTGTGGATATTCATAAATTTTATATAA

mRNA sequence

AGGGCTCAGGTTCTGGCTGATTTGTTCATTACTTTTTTAGGGTACAATTATTCCTTCATTGATTTATATATTAAATGAGCATTCATTTTCTCCAATTAATTTTGTACCAAAATTTAGTTATAGAAATTAGAATAGGATCTTCATTTCTGCTTTCTCCAGCCATAGGCATAGCCAGAGCCATGGACTTTAATGAGTAGGGAGGGTGGCTTGGCTATAAGGTTCCAGAGGCTTCGTTTTCTGTGAAAGTTTCTTTCGGCATGTACATACAAACTAAAGTGGGGCCATAGTACAAATCTGAATCAAACCCACAACCAGATTCCTCGAACCCATCACACTAATGGCTTCGGACCAGACTCTGTTCCACAACAGCCTTCTCGCCTTGTACCTCATCGGACCACCGACCGCCGTCTCCCTGAGGTTTCTCCAGGCGCCCTACGGGAAGCACAACCGCCCGGGGTGGGGCCCGACCATGTCGGTTCCGTTGGCGTGGTTCCTCATGGAAAGTCCCACCCTATGGCTGACTCTCCTACTCTTCCCCTCCGGTGATAACTCCTCCAACTACAAGTCTCTCCTTCTCATCTCCCTCTTCCTCCTCCACTATCTCAATCGCACCTGCCTCTACCCAATTCGCCTTCTCCGGAGTGGGACTCGACGGAATTCCGCCACCGGTTTCCCGGTGGTTGTGGCGCTCATGGCATTCGGGTTCAATTTGCTCAACTCTTACCTGCAGACCAGATCTGTGTCTCACTACGTGGATTACGACAGAGACGAGTGGTTTTGGTGGCGGTTTTTCGGTGGGGCGGTGGTTTTCGTGCTGGGGATGGCAGTGAATATTTGGTCGGATGGTGTTCTGGTAAAATTGAAGATGGCGGGCGGCGGATATAAGGTGCCGACGGGAGGGTGGTTTGAGTTGGTGAGTTGCCCTAATTATTTGGGAGAGATTGTGGAGTGGCTGGGCTGGGCGGTGATGTGCGGGTCATGGGCTGGGCTAGGGTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTACTTGAACAAGTTCGGGGAGGATTATCCCCGAGATAGGAAGGCCGTTATTCCCTTCCTGTATTAAGTGTCTGTATCAAAAATTAAAATTAATAAATAATCTTAAATTCTCTTATTTTAATTGATTAAAAAAAAAAATGAAAATTATTTATATTTAAAGAAATGGATGAAATTGTATTGTAACATAGTTTTGCCGGGCAGCGCCTCCCTCTTCGTAGCTTGCTTCTTTTCCATGGATATTATGGCGGAGAAACCTCAAAATGTGGCGATTCTCGAGCAATTGCGACTAGGAATTGCTCGATTTGAGCTCGTATCTTCGCCTGTAAATTCAATTTCACCTTCCAACTCAGTCGCTCCTTCTTTCCCTTTTTCTATGAATCGCGGCCGCCCACTCTTTGCTAAAATTGGACCCATGCTGCGCGCTGAATCGCCTGCATTGAGGAAAGTTGAGCACTACAAAGTCCAGAAAGTTACTGGAGATGGACGCTGCCTATTCCGTGCGCTTGCCAAAGGAATGGCTTTCAACAAGGGGATTCCTCTTCGTCCATTTGAGGAGAAAAATGATGCAGATGATTTACGCATGGCTGTGAAAGAAATTATCTGTGATAATGGCAAAGAAAGACGGCAGTATGAAGAAGCTCTCATTGCAATTACTGCTGAGGAGCCATTAGAACGCTACTGCCGACGAATTCGTACACCTAATTTCTGGGGAGGAGAGTCAGAGCTATTGGTATTGTCAAAGCTCTGCAAGCAACCTATCATTGTCTACATACCAGAGCATGAGCACAGAATGGGTTCGCGTAGCTCTAGCTTTATTCCTATTGCAGAATATGGAGCTGAGTTCAAGGGAGGAAACCCCAAGAAACCTGTGAGGTTGCTTTACAGCGGTAGGAACCACTACGATCTGCTTGTTTGAGGAGGGGTGATGTGACCTTGTTGCCATCTAAAGATGAGCCAACGAAGACATGCTCGACCAACAATGAATGGTCGGAGGAACCCAATAACACAACACCCAAAGCTCTCTTATTGGTCAACAATTCCAAACTTTTGAGTTGTTTAAACATTGTTTTTTTATGAAGAAATAAATGAATTGATGACACTTTAAGAGAAATAGGACATAAAGGTTGGATTTTTGTTGAAATTTGTTGCTTTTGGGAGATTTGTTTAATGCTCTATCTTTCACGATAGCAAGCAATATTTTATTTTGTGGATATTCATAAATTTTATATAA

Coding sequence (CDS)

ATGAAATTGTATTGTAACATAGTTTTGCCGGGCAGCGCCTCCCTCTTCGTAGCTTGCTTCTTTTCCATGGATATTATGGCGGAGAAACCTCAAAATGTGGCGATTCTCGAGCAATTGCGACTAGGAATTGCTCGATTTGAGCTCGTATCTTCGCCTGTAAATTCAATTTCACCTTCCAACTCAGTCGCTCCTTCTTTCCCTTTTTCTATGAATCGCGGCCGCCCACTCTTTGCTAAAATTGGACCCATGCTGCGCGCTGAATCGCCTGCATTGAGGAAAGTTGAGCACTACAAAGTCCAGAAAGTTACTGGAGATGGACGCTGCCTATTCCGTGCGCTTGCCAAAGGAATGGCTTTCAACAAGGGGATTCCTCTTCGTCCATTTGAGGAGAAAAATGATGCAGATGATTTACGCATGGCTGTGAAAGAAATTATCTGTGATAATGGCAAAGAAAGACGGCAGTATGAAGAAGCTCTCATTGCAATTACTGCTGAGGAGCCATTAGAACGCTACTGCCGACGAATTCGTACACCTAATTTCTGGGGAGGAGAGTCAGAGCTATTGGTATTGTCAAAGCTCTGCAAGCAACCTATCATTGTCTACATACCAGAGCATGAGCACAGAATGGGTTCGCGTAGCTCTAGCTTTATTCCTATTGCAGAATATGGAGCTGAGTTCAAGGGAGGAAACCCCAAGAAACCTGTGAGGTTGCTTTACAGCGGTAGGAACCACTACGATCTGCTTGTTTGA

Protein sequence

MKLYCNIVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV

Homology

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BLAST of Cp4.1LG03g02840 vs. ExPASy Swiss-Prot
Match: Q8GYW0 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 OS=Arabidopsis thaliana OX=3702 GN=OTU3 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 4.2e-69
Identity = 141/227 (62.11%), Postives = 166/227 (73.13%), Query Frame = 0


Query: 32  NVAILEQLRLGIARFELVSSPVNSISP--SNSVAPSFPFSMNRGRP--LFAKIGPMLRAE 91
           N  ILEQLR G ARFELVSSP  S+S   S++  P+   S  +G     FA+I   +   
Sbjct: 8   NNNILEQLRNGFARFELVSSPTASVSDSISSTSLPASFISTTKGNSYVFFARINSSMN-R 67

Query: 92  SPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVKEIICD 151
           SPA +KVE Y V +V GDGRCLFRAL KGMAFNKGI L P  E++DAD+LRMAVKE+IC+
Sbjct: 68  SPAAKKVEKYAVDRVKGDGRCLFRALVKGMAFNKGITLNPQRERDDADELRMAVKEVICN 127

Query: 152 NGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEH 211
           + KER +Y+EAL+AIT +E L+R+C+RI   +FWGGESELLVLSKLCKQPIIVYIPEHEH
Sbjct: 128 DPKEREKYKEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIVYIPEHEH 187

Query: 212 -RMGSRSSSFIPIAEYGAEFKGG-----NPKKPVRLLYSGRNHYDLL 249
            R G     FIPI EYG+EF+GG       K  VRLLYSGRNHYDLL
Sbjct: 188 GRGGGYGPGFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLL 233

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BLAST of Cp4.1LG03g02840 vs. ExPASy Swiss-Prot
Match: Q8LBZ4 (OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 OS=Arabidopsis thaliana OX=3702 GN=OTU4 PE=1 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 2.0e-18
Identity = 58/155 (37.42%), Postives = 81/155 (52.26%), Query Frame = 0


Query: 97  YKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFE--EKNDADDLRMAVKEIICDNGKERRQ 156
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V     D   +RRQ
Sbjct: 168 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRV----ADEFIQRRQ 227

Query: 157 YEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSS 216
             E  +    E   + Y R+IR P+ WGGE EL + S + + PI VY+ +       ++ 
Sbjct: 228 ETEWFV----EGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKD------DKAG 287

Query: 217 SFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 250
             I IAEYG E+       P+R+LY G  HYD L+
Sbjct: 288 GLISIAEYGQEY---GKDDPIRVLYHGFGHYDALL 304

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BLAST of Cp4.1LG03g02840 vs. ExPASy Swiss-Prot
Match: P22856 (Putative ubiquitin thioesterase L96 OS=Tipula iridescent virus OX=10490 GN=L96 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 1.3e-06
Identity = 53/183 (28.96%), Postives = 79/183 (43.17%), Query Frame = 0


Query: 52  PVNSISPSNSVAPSF--PFSMN-RGRPLFAKI-GPMLRAESPALRKVEHYKVQKVTGDGR 111
           P  S SPS +V PS   P S+  + RP   +  G     +   L +   YKV  V GDG 
Sbjct: 557 PDPSNSPSITVDPSVTPPSSVKIKKRPELPEYKGGNADRDLEILARRRGYKVIPVKGDGN 616

Query: 112 CLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEP 171
           CLFRA+ K +  N+ I       K   +DLR  V   +  + +    Y E +       P
Sbjct: 617 CLFRAVGKSLRLNQNI-------KYSHEDLRAQVVTYLTSHKEFLEPYLEYVTESGDTTP 676

Query: 172 ------LERYCRRIRTPNFWGGESELLVLSKLCKQPI-IVYIPEHEHRMGSRSSSFIPIA 224
                 +ERY + I  P  WG    L VLS++ K    ++ +     ++ S + +F P+ 
Sbjct: 677 QEYAKNVERYIKNISKPGTWGDFICLRVLSEILKVKFNLLILNTRNFQVISNNDTFKPLI 732

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BLAST of Cp4.1LG03g02840 vs. ExPASy Swiss-Prot
Match: B2RRE7 (OTU domain-containing protein 4 OS=Mus musculus OX=10090 GN=Otud4 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 8.7e-06
Identity = 47/172 (27.33%), Postives = 79/172 (45.93%), Query Frame = 0


Query: 80  IGPMLRAESPA---LRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADD 139
           +GP L  E+P    LRK+  Y+ + V  DG CLFRA+A+ +  ++   +          +
Sbjct: 16  VGP-LEDETPMDAYLRKLGLYR-KLVAKDGSCLFRAVAEQVLHSQSRHV----------E 75

Query: 140 LRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQ 199
           +RMA    +    +E R+  EA I    E   E Y +R+  P  W G+ E+  LS + ++
Sbjct: 76  VRMACIRYL----RENREKFEAFI----EGSFEEYLKRLENPQEWVGQVEISALSLMYRK 135

Query: 200 PIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLL 249
             ++Y   +        ++F              P+K V L +S  NHYD++
Sbjct: 136 DFVIYQEPNVSPSHVTENNF--------------PEK-VLLCFSNGNHYDIV 152

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BLAST of Cp4.1LG03g02840 vs. ExPASy Swiss-Prot
Match: Q01804 (OTU domain-containing protein 4 OS=Homo sapiens OX=9606 GN=OTUD4 PE=1 SV=4)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 43/158 (27.22%), Postives = 72/158 (45.57%), Query Frame = 0


Query: 91  LRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVKEIICDNGK 150
           LRK+  Y+ + V  DG CLFRA+A+ +  ++   +          ++RMA    +    +
Sbjct: 29  LRKLGLYR-KLVAKDGSCLFRAVAEQVLHSQSRHV----------EVRMACIHYL----R 88

Query: 151 ERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEHRMG 210
           E R+  EA I    E   E Y +R+  P  W G+ E+  LS + ++  I+Y         
Sbjct: 89  ENREKFEAFI----EGSFEEYLKRLENPQEWVGQVEISALSLMYRKDFIIY--------- 148

Query: 211 SRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLL 249
            R  +  P     ++    N  + V L +S  NHYD++
Sbjct: 149 -REPNVSP-----SQVTENNFPEKVLLCFSNGNHYDIV 152

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BLAST of Cp4.1LG03g02840 vs. NCBI nr
Match: XP_023527475.1 (OTU domain-containing protein At3g57810 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 504 bits (1297), Expect = 5.41e-180
Identity = 249/249 (100.00%), Postives = 249/249 (100.00%), Query Frame = 0


Query: 1   MKLYCNIVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN 60
           MKLYCNIVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN
Sbjct: 1   MKLYCNIVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN 60

Query: 61  SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFN 120
           SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFN
Sbjct: 61  SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFN 120

Query: 121 KGIPLRPFEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF 180
           KGIPLRPFEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF
Sbjct: 121 KGIPLRPFEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF 180

Query: 181 WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYS 240
           WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYS
Sbjct: 181 WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYS 240

Query: 241 GRNHYDLLV 249
           GRNHYDLLV
Sbjct: 241 GRNHYDLLV 249

[Close]

BLAST of Cp4.1LG03g02840 vs. NCBI nr
Match: XP_022934138.1 (OTU domain-containing protein At3g57810 [Cucurbita moschata])

HSP 1 Score: 494 bits (1273), Expect = 2.47e-176
Identity = 244/249 (97.99%), Postives = 246/249 (98.80%), Query Frame = 0


Query: 1   MKLYCNIVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN 60
           MKLY N+VLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN
Sbjct: 1   MKLYWNLVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN 60

Query: 61  SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFN 120
           SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRK EHYKVQKVTGDGRCLFRALAKGMAFN
Sbjct: 61  SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFN 120

Query: 121 KGIPLRPFEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF 180
           KGIPLRPFEEKNDADDLRMAVKE+ICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF
Sbjct: 121 KGIPLRPFEEKNDADDLRMAVKEVICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF 180

Query: 181 WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYS 240
           WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGG PKKPVRLLYS
Sbjct: 181 WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYS 240

Query: 241 GRNHYDLLV 249
           GRNHYDLLV
Sbjct: 241 GRNHYDLLV 249

[Close]

BLAST of Cp4.1LG03g02840 vs. NCBI nr
Match: KAG7017245.1 (Steroid 5-alpha-reductase DET2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 474 bits (1220), Expect = 1.75e-164
Identity = 235/242 (97.11%), Postives = 237/242 (97.93%), Query Frame = 0


Query: 8   VLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFP 67
           V+P  ASLFVA FFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFP
Sbjct: 254 VIPFLASLFVASFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFP 313

Query: 68  FSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRP 127
           FSMNRGRPLFAKIGPMLRAESPALRK EHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRP
Sbjct: 314 FSMNRGRPLFAKIGPMLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRP 373

Query: 128 FEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESEL 187
           FEEKNDADDLRMAVKE+ICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESEL
Sbjct: 374 FEEKNDADDLRMAVKEVICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESEL 433

Query: 188 LVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDL 247
           LVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGG PKKPVRLLYSGRNHYDL
Sbjct: 434 LVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDL 493

Query: 248 LV 249
           LV
Sbjct: 494 LV 495

[Close]

BLAST of Cp4.1LG03g02840 vs. NCBI nr
Match: KAG6580492.1 (Steroid 5-alpha-reductase DET2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 474 bits (1220), Expect = 1.75e-164
Identity = 235/242 (97.11%), Postives = 237/242 (97.93%), Query Frame = 0


Query: 8   VLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFP 67
           V+P  ASLFVA FFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFP
Sbjct: 254 VIPFLASLFVASFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFP 313

Query: 68  FSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRP 127
           FSMNRGRPLFAKIGPMLRAESPALRK EHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRP
Sbjct: 314 FSMNRGRPLFAKIGPMLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRP 373

Query: 128 FEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESEL 187
           FEEKNDADDLRMAVKE+ICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESEL
Sbjct: 374 FEEKNDADDLRMAVKEVICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESEL 433

Query: 188 LVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDL 247
           LVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGG PKKPVRLLYSGRNHYDL
Sbjct: 434 LVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDL 493

Query: 248 LV 249
           LV
Sbjct: 494 LV 495

[Close]

BLAST of Cp4.1LG03g02840 vs. NCBI nr
Match: XP_022983319.1 (OTU domain-containing protein At3g57810 [Cucurbita maxima])

HSP 1 Score: 454 bits (1168), Expect = 1.13e-160
Identity = 223/227 (98.24%), Postives = 224/227 (98.68%), Query Frame = 0


Query: 23  MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 82
           MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP
Sbjct: 1   MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 60

Query: 83  MLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 142
           MLRAESPALRK EHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  MLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 120

Query: 143 EIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 202
           E+ICDNGKERRQYEEALIAIT EEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 180

Query: 203 PEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 249
           PEHEHRMGSRSSSFIPIAEYGAEFKGG PKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of Cp4.1LG03g02840 vs. ExPASy TrEMBL
Match: A0A6J1F6U0 (Ubiquitin thioesterase OTU1 OS=Cucurbita moschata OX=3662 GN=LOC111441401 PE=4 SV=1)

HSP 1 Score: 494 bits (1273), Expect = 1.19e-176
Identity = 244/249 (97.99%), Postives = 246/249 (98.80%), Query Frame = 0


Query: 1   MKLYCNIVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN 60
           MKLY N+VLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN
Sbjct: 1   MKLYWNLVLPGSASLFVACFFSMDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSN 60

Query: 61  SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFN 120
           SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRK EHYKVQKVTGDGRCLFRALAKGMAFN
Sbjct: 61  SVAPSFPFSMNRGRPLFAKIGPMLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFN 120

Query: 121 KGIPLRPFEEKNDADDLRMAVKEIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF 180
           KGIPLRPFEEKNDADDLRMAVKE+ICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF
Sbjct: 121 KGIPLRPFEEKNDADDLRMAVKEVICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNF 180

Query: 181 WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYS 240
           WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGG PKKPVRLLYS
Sbjct: 181 WGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYS 240

Query: 241 GRNHYDLLV 249
           GRNHYDLLV
Sbjct: 241 GRNHYDLLV 249

[Close]

BLAST of Cp4.1LG03g02840 vs. ExPASy TrEMBL
Match: A0A6J1J705 (Ubiquitin thioesterase OTU1 OS=Cucurbita maxima OX=3661 GN=LOC111481933 PE=4 SV=1)

HSP 1 Score: 454 bits (1168), Expect = 5.45e-161
Identity = 223/227 (98.24%), Postives = 224/227 (98.68%), Query Frame = 0


Query: 23  MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 82
           MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP
Sbjct: 1   MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 60

Query: 83  MLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 142
           MLRAESPALRK EHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK
Sbjct: 61  MLRAESPALRKAEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 120

Query: 143 EIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 202
           E+ICDNGKERRQYEEALIAIT EEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 180

Query: 203 PEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 249
           PEHEHRMGSRSSSFIPIAEYGAEFKGG PKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHRMGSRSSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of Cp4.1LG03g02840 vs. ExPASy TrEMBL
Match: A0A5D3DN03 (Ubiquitin thioesterase OTU1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001090 PE=4 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 2.24e-144
Identity = 201/227 (88.55%), Postives = 211/227 (92.95%), Query Frame = 0


Query: 23  MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 82
           MDIMAEKP+N +ILEQL+LGIARFE  SSPV+SISPSNSVA SFP SMNRG PLFAKIGP
Sbjct: 1   MDIMAEKPRNASILEQLQLGIARFEFSSSPVSSISPSNSVASSFPLSMNRGHPLFAKIGP 60

Query: 83  MLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 142
           +L  ESPALRK E YKVQKVTGDGRCLFRALAKGMAFN+GIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 143 EIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 202
           E+ICDNGKERRQYEEALIAIT EEPLERYC+RIRTP+FWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 203 PEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 249
           P HEH+MG R  SFIPIAEYGAEFKGG PKKPVRLLYSGRNHYDLLV
Sbjct: 181 PGHEHKMGCRGHSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of Cp4.1LG03g02840 vs. ExPASy TrEMBL
Match: A0A1S3B5R5 (Ubiquitin thioesterase OTU1 OS=Cucumis melo OX=3656 GN=LOC103486327 PE=4 SV=1)

HSP 1 Score: 412 bits (1059), Expect = 2.24e-144
Identity = 201/227 (88.55%), Postives = 211/227 (92.95%), Query Frame = 0


Query: 23  MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 82
           MDIMAEKP+N +ILEQL+LGIARFE  SSPV+SISPSNSVA SFP SMNRG PLFAKIGP
Sbjct: 1   MDIMAEKPRNASILEQLQLGIARFEFSSSPVSSISPSNSVASSFPLSMNRGHPLFAKIGP 60

Query: 83  MLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 142
           +L  ESPALRK E YKVQKVTGDGRCLFRALAKGMAFN+GIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 143 EIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 202
           E+ICDNGKERRQYEEALIAIT EEPLERYC+RIRTP+FWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKERRQYEEALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 203 PEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 249
           P HEH+MG R  SFIPIAEYGAEFKGG PKKPVRLLYSGRNHYDLLV
Sbjct: 181 PGHEHKMGCRGHSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of Cp4.1LG03g02840 vs. ExPASy TrEMBL
Match: A0A0A0LCX5 (Ubiquitin thioesterase OTU1 OS=Cucumis sativus OX=3659 GN=Csa_3G732560 PE=4 SV=1)

HSP 1 Score: 410 bits (1054), Expect = 1.29e-143
Identity = 202/227 (88.99%), Postives = 211/227 (92.95%), Query Frame = 0


Query: 23  MDIMAEKPQNVAILEQLRLGIARFELVSSPVNSISPSNSVAPSFPFSMNRGRPLFAKIGP 82
           MDIMAEK QNV+ILEQL+LGIARFEL SSPV+SISPSNSVA SFP SMN   PLFAKIGP
Sbjct: 1   MDIMAEKSQNVSILEQLQLGIARFELSSSPVSSISPSNSVASSFPLSMNPSYPLFAKIGP 60

Query: 83  MLRAESPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVK 142
           +L  ESPALRK E YKVQKVTGDGRCLFRALAKGMAFN+GIPLRPFEEKNDADDLRMAVK
Sbjct: 61  VLGIESPALRKAEQYKVQKVTGDGRCLFRALAKGMAFNRGIPLRPFEEKNDADDLRMAVK 120

Query: 143 EIICDNGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYI 202
           E+ICDNGKE+RQYE ALIAIT EEPLERYC+RIRTP+FWGGESELLVLSKLCKQPIIVYI
Sbjct: 121 EVICDNGKEKRQYEAALIAITVEEPLERYCQRIRTPDFWGGESELLVLSKLCKQPIIVYI 180

Query: 203 PEHEHRMGSRSSSFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 249
           PEHEHRMG R SSFIPIAEYGAEFKGG PKKPVRLLYSGRNHYDLLV
Sbjct: 181 PEHEHRMGCRGSSFIPIAEYGAEFKGGKPKKPVRLLYSGRNHYDLLV 227

[Close]

BLAST of Cp4.1LG03g02840 vs. TAIR 10
Match: AT2G38025.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 262.7 bits (670), Expect = 3.0e-70
Identity = 141/227 (62.11%), Postives = 166/227 (73.13%), Query Frame = 0


Query: 32  NVAILEQLRLGIARFELVSSPVNSISP--SNSVAPSFPFSMNRGRP--LFAKIGPMLRAE 91
           N  ILEQLR G ARFELVSSP  S+S   S++  P+   S  +G     FA+I   +   
Sbjct: 8   NNNILEQLRNGFARFELVSSPTASVSDSISSTSLPASFISTTKGNSYVFFARINSSMN-R 67

Query: 92  SPALRKVEHYKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFEEKNDADDLRMAVKEIICD 151
           SPA +KVE Y V +V GDGRCLFRAL KGMAFNKGI L P  E++DAD+LRMAVKE+IC+
Sbjct: 68  SPAAKKVEKYAVDRVKGDGRCLFRALVKGMAFNKGITLNPQRERDDADELRMAVKEVICN 127

Query: 152 NGKERRQYEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEH 211
           + KER +Y+EAL+AIT +E L+R+C+RI   +FWGGESELLVLSKLCKQPIIVYIPEHEH
Sbjct: 128 DPKEREKYKEALVAITVDESLKRFCQRIGRHDFWGGESELLVLSKLCKQPIIVYIPEHEH 187

Query: 212 -RMGSRSSSFIPIAEYGAEFKGG-----NPKKPVRLLYSGRNHYDLL 249
            R G     FIPI EYG+EF+GG       K  VRLLYSGRNHYDLL
Sbjct: 188 GRGGGYGPGFIPIQEYGSEFRGGWGKGKTNKNVVRLLYSGRNHYDLL 233

[Close]

BLAST of Cp4.1LG03g02840 vs. TAIR 10
Match: AT3G57810.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.4e-19
Identity = 58/155 (37.42%), Postives = 81/155 (52.26%), Query Frame = 0


Query: 97  YKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFE--EKNDADDLRMAVKEIICDNGKERRQ 156
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V     D   +RRQ
Sbjct: 125 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRV----ADEFIQRRQ 184

Query: 157 YEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSS 216
             E  +    E   + Y R+IR P+ WGGE EL + S + + PI VY+ +       ++ 
Sbjct: 185 ETEWFV----EGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKD------DKAG 244

Query: 217 SFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 250
             I IAEYG E+       P+R+LY G  HYD L+
Sbjct: 245 GLISIAEYGQEY---GKDDPIRVLYHGFGHYDALL 261

[Close]

BLAST of Cp4.1LG03g02840 vs. TAIR 10
Match: AT3G57810.2 (Cysteine proteinases superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.4e-19
Identity = 58/155 (37.42%), Postives = 81/155 (52.26%), Query Frame = 0


Query: 97  YKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFE--EKNDADDLRMAVKEIICDNGKERRQ 156
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V     D   +RRQ
Sbjct: 168 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRV----ADEFIQRRQ 227

Query: 157 YEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSS 216
             E  +    E   + Y R+IR P+ WGGE EL + S + + PI VY+ +       ++ 
Sbjct: 228 ETEWFV----EGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKD------DKAG 287

Query: 217 SFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 250
             I IAEYG E+       P+R+LY G  HYD L+
Sbjct: 288 GLISIAEYGQEY---GKDDPIRVLYHGFGHYDALL 304

[Close]

BLAST of Cp4.1LG03g02840 vs. TAIR 10
Match: AT3G57810.3 (Cysteine proteinases superfamily protein )

HSP 1 Score: 94.4 bits (233), Expect = 1.4e-19
Identity = 58/155 (37.42%), Postives = 81/155 (52.26%), Query Frame = 0


Query: 97  YKVQKVTGDGRCLFRALAKGMAFNKGIPLRPFE--EKNDADDLRMAVKEIICDNGKERRQ 156
           Y +  + GDGRCLFR++A G     G  L P E  ++  AD+LR  V     D   +RRQ
Sbjct: 168 YSIIGIPGDGRCLFRSVAHGFCLRSG-KLAPGEKMQRELADELRTRV----ADEFIQRRQ 227

Query: 157 YEEALIAITAEEPLERYCRRIRTPNFWGGESELLVLSKLCKQPIIVYIPEHEHRMGSRSS 216
             E  +    E   + Y R+IR P+ WGGE EL + S + + PI VY+ +       ++ 
Sbjct: 228 ETEWFV----EGDFDTYVRQIRDPHVWGGEPELFMASHVLQMPITVYMKD------DKAG 287

Query: 217 SFIPIAEYGAEFKGGNPKKPVRLLYSGRNHYDLLV 250
             I IAEYG E+       P+R+LY G  HYD L+
Sbjct: 288 GLISIAEYGQEY---GKDDPIRVLYHGFGHYDALL 304

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g02840 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GYW0	4.2e-69	62.11	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 3 OS=Arabidopsis thalian...	[more]
Q8LBZ4	2.0e-18	37.42	OVARIAN TUMOR DOMAIN-containing deubiquitinating enzyme 4 OS=Arabidopsis thalian...	[more]
P22856	1.3e-06	28.96	Putative ubiquitin thioesterase L96 OS=Tipula iridescent virus OX=10490 GN=L96 P...	[more]
B2RRE7	8.7e-06	27.33	OTU domain-containing protein 4 OS=Mus musculus OX=10090 GN=Otud4 PE=1 SV=1	[more]
Q01804	1.1e-05	27.22	OTU domain-containing protein 4 OS=Homo sapiens OX=9606 GN=OTUD4 PE=1 SV=4	[more]

BLAST of Cp4.1LG03g02840 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023527475.1	5.41e-180	100.00	OTU domain-containing protein At3g57810 [Cucurbita pepo subsp. pepo]	[more]
XP_022934138.1	2.47e-176	97.99	OTU domain-containing protein At3g57810 [Cucurbita moschata]	[more]
KAG7017245.1	1.75e-164	97.11	Steroid 5-alpha-reductase DET2, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
KAG6580492.1	1.75e-164	97.11	Steroid 5-alpha-reductase DET2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022983319.1	1.13e-160	98.24	OTU domain-containing protein At3g57810 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG03g02840 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F6U0	1.19e-176	97.99	Ubiquitin thioesterase OTU1 OS=Cucurbita moschata OX=3662 GN=LOC111441401 PE=4 S...	[more]
A0A6J1J705	5.45e-161	98.24	Ubiquitin thioesterase OTU1 OS=Cucurbita maxima OX=3661 GN=LOC111481933 PE=4 SV=...	[more]
A0A5D3DN03	2.24e-144	88.55	Ubiquitin thioesterase OTU1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3B5R5	2.24e-144	88.55	Ubiquitin thioesterase OTU1 OS=Cucumis melo OX=3656 GN=LOC103486327 PE=4 SV=1	[more]
A0A0A0LCX5	1.29e-143	88.99	Ubiquitin thioesterase OTU1 OS=Cucumis sativus OX=3659 GN=Csa_3G732560 PE=4 SV=1	[more]

BLAST of Cp4.1LG03g02840 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38025.1	3.0e-70	62.11	Cysteine proteinases superfamily protein	[more]
AT3G57810.1	1.4e-19	37.42	Cysteine proteinases superfamily protein	[more]
AT3G57810.2	1.4e-19	37.42	Cysteine proteinases superfamily protein	[more]
AT3G57810.3	1.4e-19	37.42	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003323	OTU domain	PFAM	PF02338	OTU	coord: 103..241 e-value: 1.4E-12 score: 48.2
IPR003323	OTU domain	PROSITE	PS50802	OTU	coord: 97..249 score: 16.128559
None	No IPR available	GENE3D	3.90.70.80		coord: 77..249 e-value: 3.6E-35 score: 123.3
None	No IPR available	PANTHER	PTHR13312:SF3	OTU-LIKE CYSTEINE PROTEASE FAMILY PROTEIN, EXPRESSED	coord: 27..249
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..19 score: 5.0
IPR039138	Ubiquitin thioesterase OTU1/2/3	PANTHER	PTHR13312	HIV-INDUCED PROTEIN-7-LIKE PROTEASE	coord: 27..249
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 93..249

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g02840.1	Cp4.1LG03g02840.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030968	endoplasmic reticulum unfolded protein response
biological_process	GO:0016579	protein deubiquitination
biological_process	GO:0030433	ubiquitin-dependent ERAD pathway
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004843	thiol-dependent deubiquitinase
molecular_function	GO:0101005	deubiquitinase activity