Cp4.1LG02g17540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g17540
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	(S)-2-hydroxy-acid oxidase
Location	Cp4.1LG02: 14330725 .. 14332719 (+)
RNA-Seq Expression	Cp4.1LG02g17540
Synteny	Cp4.1LG02g17540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTTCAAAAGGCGTGATATCACGGATTTGATAGTACAGAGAGCTGAGACATGTGGATACAAGGCCATTGTGGTGACTCTTGATACTCCTTGGCTTGGTCGAAGGGAGGCTGACATGAAGAACAATTAAGTATCTATGCCTCACATCTCATGCATTGTATGTATTCCTTTTGCTGGGTATTGAGTGCTTCAATTGTAAGCTACGCCTTTGTCTTTGTCTCATCTGGAAAATCAATTGGATGAATTTGTAGGATGATTGCATATCCAAAGAAGAATCTTGAAGGCCTCATGTCTGTTGAAATTAACTCTGTGAGTAATGGTTCAATTTCCTTGTTGAGAATATTGGAATTACATCTCACCATTAAATCTAGATATATTTGTTAGGAATCATGACTCTTCAAAATAGTATTGTATTGTCCACTTTGAACACTAATCTCTTATTACTTTGCTTTGGACTTCTTCAAAAGGCTTTGTACTAATGAAGATAGTATTGTATTGTCCACTTTGAACACTAAGCTCTCATGGTTTTGCTTTGGACTTCTTCAAAAGGCCTCGTACTAATGAAGATGTATTCCTTGCTTATAAATCCATGATGATTCCTTAAATTAGCGAATGTGGGACTCCTTCCCAACCATCCTCATCAGTATTTTTAAGGATGTGTTTTATAATAAATATCAACTCACAATCTCATGCAGGAACCCGGTGTACAATTACGAACTTTTGCGAAAGCAACTGTGGACGCATCTTTATCCTGGAAAGTAAGTACAGAAAGTTGCTCAGATTCCTATCACACACTTGAATGTGTGCTTTACTTTGGTGCTTAATAAGCTTTGGAGCATATACTTCATATAACTCAGTAATTTAACTACTCCTATGTTGTATGGACTTCTTTCAACTACGGTAATGTAATGAATATACAGTGTTTGAGAAATCTTTCGAATCATCATCTTGATTAGATGAGATTTTCTGCTACTTGAAAGGTTTATTCTTCTAACATTCATGTTCTTCCAGTTCAGTTTTAATATTTTTCTGTTCACAGGATATGAAGTGGTTAAGATCAATCACTAGTCTGCCAATTCTGTTAAAGGGGATTCTCACTCGTGAAGATGGTAAGTTAAGTTAAGTTAAGTTTTTAAGTGTTAGGAGAAAACTAATAAATACTACGCCCGAAGCGATAAACACAAGTAGCAATTATCAAGTACGTATCAGATACTGTGATGGCCCAAGCCTACTGCTAGTAGATATTGTCTTCTTTGGGCTTTCTCTTAAGGCTTTAATACGCATTTGTTAGAAAAATGTTTCTACACCCTTATAAAGGGTGTTTCGTTCTCCTCCCCATCCAATGTGGGATATTACAATCCACCCCCTTTAGGGCCTAGTGCAATCGCTGACACTCGTTCCTTTCTCCCATCGATGTGGGACCCCCACCAAATCACCCCTATTCGGGGCCCAGCGTCCTTACTGGCACACCACCTCGTGTCTACTCCGTTCGGAGAATAACCTCCTCGCTGGCACATCACCCAGTGTGATACGATGAATGAAGTAAAAGATGTGATGAGTTGTAGAAATGCTTATCCATCTCAGACAGCACAATTTTCCTTTCAATATATTTCCTTGCTTGGTTTATGAGTTTTGTCCGAGTTCATGGATAGGCAATTTCTTTCATTAGCTTTCTTGATGATTGGCAGCAACTAAAGCTGTGGAAGCTGGTGTTGATGGAATTATTGTCTCCAATCATGGAGGCCGCCAACTAGACTTTACTCCGCCCACTATTCTCGTTCTCGAAGAGGTCAGTTTCGCTTCATTTTGAGCTACTATATTGCTCAAAGATGTGAATGCAAGGAGATAAAATTACTTCAATTTCTGCATATGTTAGCTGGAAATGCTGAAGAATGAGTTAGAGACCACCATGGCACTCTCAGGTTGCCCATCTATCAGGGATATTACTAGAAACCATGTTAGGACACAGTATGATAAGCTGCAAGCAATGCTTTGA

mRNA sequence

GTTTTCAAAAGGCGTGATATCACGGATTTGATAGTACAGAGAGCTGAGACATGTGGATACAAGGCCATTGTGGTGACTCTTGATACTCCTTGGCTTGGTCGAAGGGAGGCTGACATGAAGAACAATTAAGTATCTATGCCTCACATCTCATGCATTCAACTGTGGACGCATCTTTATCCTGGAAAGATATGAAGTGGTTAAGATCAATCACTAGTCTGCCAATTCTGTTAAAGGGGATTCTCACTCGTGAAGATGCAACTAAAGCTGTGGAAGCTGGTGTTGATGGAATTATTGTCTCCAATCATGGAGGCCGCCAACTAGACTTTACTCCGCCCACTATTCTCGTTCTCGAAGAGCTGGAAATGCTGAAGAATGAGTTAGAGACCACCATGGCACTCTCAGGTTGCCCATCTATCAGGGATATTACTAGAAACCATGTTAGGACACAGTATGATAAGCTGCAAGCAATGCTTTGA

Coding sequence (CDS)

ATGCATTCAACTGTGGACGCATCTTTATCCTGGAAAGATATGAAGTGGTTAAGATCAATCACTAGTCTGCCAATTCTGTTAAAGGGGATTCTCACTCGTGAAGATGCAACTAAAGCTGTGGAAGCTGGTGTTGATGGAATTATTGTCTCCAATCATGGAGGCCGCCAACTAGACTTTACTCCGCCCACTATTCTCGTTCTCGAAGAGCTGGAAATGCTGAAGAATGAGTTAGAGACCACCATGGCACTCTCAGGTTGCCCATCTATCAGGGATATTACTAGAAACCATGTTAGGACACAGTATGATAAGCTGCAAGCAATGCTTTGA

Protein sequence

MHSTVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEELEMLKNELETTMALSGCPSIRDITRNHVRTQYDKLQAML

Homology

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy Swiss-Prot
Match: Q24JJ8 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=GLO3 PE=2 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 7.3e-34
Identity = 81/158 (51.27%), Postives = 93/158 (58.86%), Query Frame = 0


Query: 6   DASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTIL 65
           DAS SWKD++WLRSIT LPIL+KGILTREDA KAVEAGVDGIIVSNHGGRQLD++P TI 
Sbjct: 206 DASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGVDGIIVSNHGGRQLDYSPATIT 265

Query: 66  VLEE-------------------------------------------------------L 109
           VLEE                                                       +
Sbjct: 266 VLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVLIGRPIIYGLAAKGEDGVKKVI 325

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy Swiss-Prot
Match: Q9LJH5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=GLO4 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.1e-33
Identity = 78/161 (48.45%), Postives = 95/161 (59.01%), Query Frame = 0


Query: 3   STVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPP 62
           S  DASLSWKD++WLRSIT LPIL+KG+LTREDA KAVEAGVDGI+VSNHG RQLD++P 
Sbjct: 203 SAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGVDGIVVSNHGARQLDYSPA 262

Query: 63  TILVLEE----------------------------------------------------- 109
           TI VLEE                                                     
Sbjct: 263 TITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVLIGRPIVYGLAAKGEDGVK 322

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy Swiss-Prot
Match: B8B8K5 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=39946 GN=GLO4 PE=3 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 1.9e-26
Identity = 67/161 (41.61%), Postives = 87/161 (54.04%), Query Frame = 0


Query: 3   STVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPP 62
           +T+D SLSWKD++WL+SITS+PI LKGI+T EDA +AVEAGV G+IVSNHG RQLD+ P 
Sbjct: 206 ATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEAGVAGVIVSNHGARQLDYAPA 265

Query: 63  TILVLEE----------------------------------------------------- 109
           TI  LEE                                                     
Sbjct: 266 TIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARAVMXXXPVFFGLAARGEAGAR 325

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy Swiss-Prot
Match: Q8H3I4 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=39947 GN=GLO4 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 1.9e-26
Identity = 67/161 (41.61%), Postives = 87/161 (54.04%), Query Frame = 0


Query: 3   STVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPP 62
           +T+D SLSWKD++WL+SITS+PI LKGI+T EDA +AVEAGV G+IVSNHG RQLD+ P 
Sbjct: 206 ATLDPSLSWKDIEWLKSITSMPIFLKGIVTAEDARRAVEAGVAGVIVSNHGARQLDYAPA 265

Query: 63  TILVLEE----------------------------------------------------- 109
           TI  LEE                                                     
Sbjct: 266 TIAALEEVVRAVAGAVPVLVDGGIRRGTDVFKALALGARAVMVGRPVFFGLAARGEAGAR 325

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy Swiss-Prot
Match: O49506 (Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Arabidopsis thaliana OX=3702 GN=GLO5 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.6e-25
Identity = 66/153 (43.14%), Postives = 82/153 (53.59%), Query Frame = 0


Query: 5   VDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTI 64
           VD SLSWKD+KWL+SITSLPIL+KG++T EDA  AVE G  GIIVSNHG RQLD+ P TI
Sbjct: 207 VDQSLSWKDIKWLQSITSLPILVKGVITAEDARIAVEYGAAGIIVSNHGARQLDYVPATI 266

Query: 65  LVLEE------------------------------------------------------- 103
           + LEE                                                       
Sbjct: 267 VALEEVVKAVEGRIPVFLDGGVRRGTDVFKALALGASGVFVGRPSLFSLAADGEAGVRKM 326

[Close]

BLAST of Cp4.1LG02g17540 vs. NCBI nr
Match: XP_022998201.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 182 bits (461), Expect = 7.34e-54
Identity = 102/161 (63.35%), Postives = 106/161 (65.84%), Query Frame = 0


Query: 3   STVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPP 62
           +TVDASLSWKD++WLRSITSLPILLKGILTREDATKAVEAGVDGI+VSNHGGRQLDFTPP
Sbjct: 204 ATVDASLSWKDIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQLDFTPP 263

Query: 63  TILVLEE----------------------------------------------------- 108
           TILVLEE                                                     
Sbjct: 264 TILVLEEVVQAVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKGEEGAR 323

[Close]

BLAST of Cp4.1LG02g17540 vs. NCBI nr
Match: XP_022998202.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 163 bits (412), Expect = 9.66e-47
Identity = 93/151 (61.59%), Postives = 96/151 (63.58%), Query Frame = 0


Query: 13  DMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEE--- 72
           D++WLRSITSLPILLKGILTREDATKAVEAGVDGI+VSNHGGRQLDFTPPTILVLEE   
Sbjct: 193 DIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQLDFTPPTILVLEEVVQ 252

Query: 73  ----------------------------------------------------LEMLKNEL 108
                                                               LEMLKNEL
Sbjct: 253 AVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKGEEGARTVLEMLKNEL 312

[Close]

BLAST of Cp4.1LG02g17540 vs. NCBI nr
Match: XP_022998200.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima] >XP_022998204.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 163 bits (412), Expect = 3.31e-46
Identity = 93/151 (61.59%), Postives = 96/151 (63.58%), Query Frame = 0


Query: 13  DMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEE--- 72
           D++WLRSITSLPILLKGILTREDATKAVEAGVDGI+VSNHGGRQLDFTPPTILVLEE   
Sbjct: 247 DIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQLDFTPPTILVLEEVVQ 306

Query: 73  ----------------------------------------------------LEMLKNEL 108
                                                               LEMLKNEL
Sbjct: 307 AVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKGEEGARTVLEMLKNEL 366

[Close]

BLAST of Cp4.1LG02g17540 vs. NCBI nr
Match: XP_031738756.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X7 [Cucumis sativus])

HSP 1 Score: 152 bits (383), Expect = 2.66e-43
Identity = 84/162 (51.85%), Postives = 97/162 (59.88%), Query Frame = 0


Query: 2   HSTVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTP 61
           + T+D S+ W+D++WLRSIT+LPIL+KGILT EDATKAVEAGVDGIIVSNHG RQLDF P
Sbjct: 99  NKTLDDSMRWEDIQWLRSITTLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFAP 158

Query: 62  PTILVLEE---------------------------------------------------- 108
            T+ VLEE                                                    
Sbjct: 159 ATVTVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLYGLAAKGEEGV 218

[Close]

BLAST of Cp4.1LG02g17540 vs. NCBI nr
Match: XP_031738752.1 (peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X6 [Cucumis sativus] >XP_031738753.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X6 [Cucumis sativus] >XP_031738754.1 peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X6 [Cucumis sativus])

HSP 1 Score: 152 bits (383), Expect = 7.31e-43
Identity = 84/162 (51.85%), Postives = 97/162 (59.88%), Query Frame = 0


Query: 2   HSTVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTP 61
           + T+D S+ W+D++WLRSIT+LPIL+KGILT EDATKAVEAGVDGIIVSNHG RQLDF P
Sbjct: 138 NKTLDDSMRWEDIQWLRSITTLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFAP 197

Query: 62  PTILVLEE---------------------------------------------------- 108
            T+ VLEE                                                    
Sbjct: 198 ATVTVLEEVVHAVKGKVPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLYGLAAKGEEGV 257

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy TrEMBL
Match: A0A6J1K9L4 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1)

HSP 1 Score: 182 bits (461), Expect = 3.55e-54
Identity = 102/161 (63.35%), Postives = 106/161 (65.84%), Query Frame = 0


Query: 3   STVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPP 62
           +TVDASLSWKD++WLRSITSLPILLKGILTREDATKAVEAGVDGI+VSNHGGRQLDFTPP
Sbjct: 204 ATVDASLSWKDIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQLDFTPP 263

Query: 63  TILVLEE----------------------------------------------------- 108
           TILVLEE                                                     
Sbjct: 264 TILVLEEVVQAVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKGEEGAR 323

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy TrEMBL
Match: A0A6J1K7A3 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1)

HSP 1 Score: 163 bits (412), Expect = 4.68e-47
Identity = 93/151 (61.59%), Postives = 96/151 (63.58%), Query Frame = 0


Query: 13  DMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEE--- 72
           D++WLRSITSLPILLKGILTREDATKAVEAGVDGI+VSNHGGRQLDFTPPTILVLEE   
Sbjct: 193 DIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQLDFTPPTILVLEEVVQ 252

Query: 73  ----------------------------------------------------LEMLKNEL 108
                                                               LEMLKNEL
Sbjct: 253 AVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKGEEGARTVLEMLKNEL 312

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy TrEMBL
Match: A0A6J1KDR0 ((S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1)

HSP 1 Score: 163 bits (412), Expect = 1.60e-46
Identity = 93/151 (61.59%), Postives = 96/151 (63.58%), Query Frame = 0


Query: 13  DMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTILVLEE--- 72
           D++WLRSITSLPILLKGILTREDATKAVEAGVDGI+VSNHGGRQLDFTPPTILVLEE   
Sbjct: 247 DIEWLRSITSLPILLKGILTREDATKAVEAGVDGILVSNHGGRQLDFTPPTILVLEEVVQ 306

Query: 73  ----------------------------------------------------LEMLKNEL 108
                                                               LEMLKNEL
Sbjct: 307 AVKGKVPVLLDGGVRRGTDIFKALALGAQAVLTGRAVLFGLSAKGEEGARTVLEMLKNEL 366

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy TrEMBL
Match: A0A6J1D4Y8 ((S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016982 PE=3 SV=1)

HSP 1 Score: 152 bits (385), Expect = 8.14e-43
Identity = 87/162 (53.70%), Postives = 98/162 (60.49%), Query Frame = 0


Query: 2   HSTVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTP 61
           ++T+DASL W+D+ WLRSITSLPIL+KGILT EDAT+AVEAGVDGIIVSNHG RQLDF P
Sbjct: 203 NATLDASLRWEDIGWLRSITSLPILIKGILTHEDATRAVEAGVDGIIVSNHGARQLDFAP 262

Query: 62  PTILVLEE---------------------------------------------------- 108
            T+ VLEE                                                    
Sbjct: 263 ATVSVLEEVVHAVKGRVPVLFDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEEGV 322

[Close]

BLAST of Cp4.1LG02g17540 vs. ExPASy TrEMBL
Match: A0A6J1GUP4 ((S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV=1)

HSP 1 Score: 148 bits (374), Expect = 8.75e-42
Identity = 85/159 (53.46%), Postives = 93/159 (58.49%), Query Frame = 0


Query: 5   VDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTI 64
           +DASL W+D+ WLRSITSLPIL+KGILT EDATKAVEAGVDGIIVSNHG RQLDF P TI
Sbjct: 145 LDASLRWEDISWLRSITSLPILIKGILTHEDATKAVEAGVDGIIVSNHGARQLDFAPATI 204

Query: 65  LVLEE------------------------------------------------------- 108
            VLEE                                                       
Sbjct: 205 SVLEEVVHAVKGKIPVLLDGGVRRGTDVFKALALGAQAVLIGRPVLFGLAAKGEGGVRTV 264

[Close]

BLAST of Cp4.1LG02g17540 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 144.4 bits (363), Expect = 5.2e-35
Identity = 81/158 (51.27%), Postives = 93/158 (58.86%), Query Frame = 0


Query: 6   DASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTIL 65
           DAS SWKD++WLRSIT LPIL+KGILTREDA KAVEAGVDGIIVSNHGGRQLD++P TI 
Sbjct: 206 DASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGVDGIIVSNHGGRQLDYSPATIT 265

Query: 66  VLEE-------------------------------------------------------L 109
           VLEE                                                       +
Sbjct: 266 VLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVLIGRPIIYGLAAKGEDGVKKVI 325

[Close]

BLAST of Cp4.1LG02g17540 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 144.4 bits (363), Expect = 5.2e-35
Identity = 81/158 (51.27%), Postives = 93/158 (58.86%), Query Frame = 0


Query: 6   DASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTIL 65
           DAS SWKD++WLRSIT LPIL+KGILTREDA KAVEAGVDGIIVSNHGGRQLD++P TI 
Sbjct: 206 DASFSWKDIEWLRSITELPILVKGILTREDALKAVEAGVDGIIVSNHGGRQLDYSPATIT 265

Query: 66  VLEE-------------------------------------------------------L 109
           VLEE                                                       +
Sbjct: 266 VLEEVVQVVRGRIPVLLDGGVRRGTDVFKALALGAQAVLIGRPIIYGLAAKGEDGVKKVI 325

[Close]

BLAST of Cp4.1LG02g17540 vs. TAIR 10
Match: AT3G14130.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 142.9 bits (359), Expect = 1.5e-34
Identity = 78/161 (48.45%), Postives = 95/161 (59.01%), Query Frame = 0


Query: 3   STVDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPP 62
           S  DASLSWKD++WLRSIT LPIL+KG+LTREDA KAVEAGVDGI+VSNHG RQLD++P 
Sbjct: 203 SAFDASLSWKDIEWLRSITKLPILVKGLLTREDALKAVEAGVDGIVVSNHGARQLDYSPA 262

Query: 63  TILVLEE----------------------------------------------------- 109
           TI VLEE                                                     
Sbjct: 263 TITVLEEVVHAVKGRIPVLLDGGVRRGTDVFKALALGAQAVLIGRPIVYGLAAKGEDGVK 322

[Close]

BLAST of Cp4.1LG02g17540 vs. TAIR 10
Match: AT4G18360.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 116.7 bits (291), Expect = 1.2e-26
Identity = 66/153 (43.14%), Postives = 82/153 (53.59%), Query Frame = 0


Query: 5   VDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTI 64
           VD SLSWKD+KWL+SITSLPIL+KG++T EDA  AVE G  GIIVSNHG RQLD+ P TI
Sbjct: 207 VDQSLSWKDIKWLQSITSLPILVKGVITAEDARIAVEYGAAGIIVSNHGARQLDYVPATI 266

Query: 65  LVLEE------------------------------------------------------- 103
           + LEE                                                       
Sbjct: 267 VALEEVVKAVEGRIPVFLDGGVRRGTDVFKALALGASGVFVGRPSLFSLAADGEAGVRKM 326

[Close]

BLAST of Cp4.1LG02g17540 vs. TAIR 10
Match: AT3G14420.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 111.7 bits (278), Expect = 3.7e-25
Identity = 61/153 (39.87%), Postives = 82/153 (53.59%), Query Frame = 0


Query: 5   VDASLSWKDMKWLRSITSLPILLKGILTREDATKAVEAGVDGIIVSNHGGRQLDFTPPTI 64
           +D +LSWKD++WL++IT LPIL+KG+LT EDA  A++AG  GIIVSNHG RQLD+ P TI
Sbjct: 207 IDRTLSWKDVQWLQTITKLPILVKGVLTGEDARIAIQAGAAGIIVSNHGARQLDYVPATI 266

Query: 65  LVLEE------------------------------------------------------- 103
             LEE                                                       
Sbjct: 267 SALEEVVKATQGRIPVFLDGGVRRGTDVFKALALGASGIFIGRPVVFSLAAEGEAGVRKV 326

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g17540 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q24JJ8	7.3e-34	51.27	Peroxisomal (S)-2-hydroxy-acid oxidase GLO3 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LJH5	2.1e-33	48.45	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
B8B8K5	1.9e-26	41.61	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. indica OX=399...	[more]
Q8H3I4	1.9e-26	41.61	Peroxisomal (S)-2-hydroxy-acid oxidase GLO4 OS=Oryza sativa subsp. japonica OX=3...	[more]
O49506	1.6e-25	43.14	Peroxisomal (S)-2-hydroxy-acid oxidase GLO5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]

BLAST of Cp4.1LG02g17540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022998201.1	7.34e-54	63.35	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X2 [Cucurbita maxima]	[more]
XP_022998202.1	9.66e-47	61.59	peroxisomal (S)-2-hydroxy-acid oxidase GLO3-like isoform X3 [Cucurbita maxima]	[more]
XP_022998200.1	3.31e-46	61.59	peroxisomal (S)-2-hydroxy-acid oxidase GLO4-like isoform X1 [Cucurbita maxima] >...	[more]
XP_031738756.1	2.66e-43	51.85	peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X7 [Cucumis sativus]	[more]
XP_031738752.1	7.31e-43	51.85	peroxisomal (S)-2-hydroxy-acid oxidase GLO4 isoform X6 [Cucumis sativus] >XP_031...	[more]

BLAST of Cp4.1LG02g17540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K9L4	3.55e-54	63.35	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1	[more]
A0A6J1K7A3	4.68e-47	61.59	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1	[more]
A0A6J1KDR0	1.60e-46	61.59	(S)-2-hydroxy-acid oxidase OS=Cucurbita maxima OX=3661 GN=LOC111492921 PE=3 SV=1	[more]
A0A6J1D4Y8	8.14e-43	53.70	(S)-2-hydroxy-acid oxidase OS=Momordica charantia OX=3673 GN=LOC111016982 PE=3 S...	[more]
A0A6J1GUP4	8.75e-42	53.46	(S)-2-hydroxy-acid oxidase OS=Cucurbita moschata OX=3662 GN=LOC111457615 PE=3 SV...	[more]

BLAST of Cp4.1LG02g17540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14150.1	5.2e-35	51.27	Aldolase-type TIM barrel family protein	[more]
AT3G14150.2	5.2e-35	51.27	Aldolase-type TIM barrel family protein	[more]
AT3G14130.1	1.5e-34	48.45	Aldolase-type TIM barrel family protein	[more]
AT4G18360.1	1.2e-26	43.14	Aldolase-type TIM barrel family protein	[more]
AT3G14420.1	3.7e-25	39.87	Aldolase-type TIM barrel family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..69 e-value: 1.9E-30 score: 107.8 coord: 70..108 e-value: 1.1E-7 score: 32.9
IPR000262	FMN-dependent dehydrogenase	PFAM	PF01070	FMN_dh	coord: 3..70 e-value: 3.8E-29 score: 101.8
None	No IPR available	PANTHER	PTHR10578	S -2-HYDROXY-ACID OXIDASE-RELATED	coord: 70..103 coord: 5..69
None	No IPR available	PANTHER	PTHR10578:SF131	BNAC05G52090D PROTEIN	coord: 70..103 coord: 5..69
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 4..97
IPR008259	FMN-dependent alpha-hydroxy acid dehydrogenase, active site	PROSITE	PS00557	FMN_HYDROXY_ACID_DH_1	coord: 50..56
IPR037396	FMN hydroxy acid dehydrogenase domain	PROSITE	PS51349	FMN_HYDROXY_ACID_DH_2	coord: 1..108 score: 27.025932

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g17540.1	Cp4.1LG02g17540.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003824	catalytic activity