Cp4.1LG02g16780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g16780
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	RING-type E3 ubiquitin transferase
Location	Cp4.1LG02: 12627720 .. 12630480 (-)
RNA-Seq Expression	Cp4.1LG02g16780
Synteny	Cp4.1LG02g16780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTAAAATGAACCTTTATTGATACGACCGCAGATAAAGTAGAAAAGAAATGAGAAATATAAAAATGTTTTTTTAAAATCAAATGACGAAACTACCCCTCAATTTCGGAATAAAACTGTTTTTTTCACGACGGAAAGACTTAGATGATCTGCCAAGTCTTCGTCCTCACGGTCTGACGAAGAGCAGAGACTAACTTGAAGAAGAACAAATGGCGGATTCTCGATCATCGGACGGCGAAAAGGAGAGGCGACGTTCGGTCAACTGTAGCAGAGGGAAGAATGAGGCGATGAAAGTCATATTCACTGATCCTCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTACCAGGTCCGATTAAGCTTTTTGATCGCCTGTATTTTGCTTGCAATTATCGTTATTATTTATCTGTTTGTCTCTGATGCTTTGTTCTTCTCGTCTTTTTTGTTTTAGTTTGTATTTTTAAAATGTTATTTTTTGTTTATTTTGTTTANCTACTGTAGTTCACTTAGTGTTTTTGCTGGAATTGACGATTTTGGAATCCTCCTTTCTATGAGGTTTTTACCTTACCGTTTTGATTTTTTTTTTTGTGTAAATCCACAGAAGTTATGGATTATGATAACAATTGAAGAAATATAGCGAAGTTTTGAACGGTTATTCATTGCAACGTTGTCTAATTTGAGCATAAGCTCTCGGACCTTGTTTTTGGTTTTCCAAAAGGCCTCGTACCAACGGAAATAGTGTTACTTACCTGTAAAGTCATGATTTTCCTCTTAATTAGCTAATCTAGACTACTCCTTTCAATAATCCTCAACAATCCTCCCATCGAACAAACGACACCATAGAGTCTCCTCTGAGGCCTATAGAGCCATCGAACAACCTCTCTTTAATTGAGGCTGGACTCCTTTCTTTGGAGCCTCTAAACAAAATACACCTTTTGTTCGACACTTGAGTCACTTTTGACTACACCTTCGAAACTCACTTCTTTGTTCGACACTTGAGGATTCTATTGATATAGCCAAGTTAAGGGCATAACTCTTATATAATGTTAGGAATCACGAACCTCCACAATGATATGATATTGTCCACTCTGAGCATAAGCTCTCGACTTTACTTTTGGTTTCTCCAAAAGGGCTCGTACCATGGAAAACCATGAAAATTTACTTATAAACTCATGATCTTACTCTTAATTAGCCAACGTGAGACTTCCAATAATCTTGAAAACTGAAGTTATTAAAGGTTTTGATTCAAATAATCTTTGAACATGGAGATTATTCTGGATATTACGGGGACAGAGTTGTTAAATGGATGGGATTGAAATGAAATCTTGACCTGAATTGCTTATCTTGTTACATTGTCTCGAGAAAATGTCAGAATCAAGTGGTGCTTGATATTCTTTTCACCTTTCTGTGCCTGACTTGGGGTTCAATCGTGTGGACTGTGGTGTTGGATGAGGGAAGTCCCACATCGACTAATTTAGGGAATGATCATGGGTTTATAAGTGAGGAATACTAACTCCATTGGAATGAGGCATTTTGGAGAAGCCCAAAACAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTCGTCTAACATGTGAGATTTTCTTCATTGTTTACAAACATGAATTTGAAAGGGATGAATCTAGAAGAAAATAAGCTTATATCTCATTTTGTTTTCTATGAGTCTATTTTATTTCATAACATATACTCTGGTGTTGAGATGTTGGTTGTTGGATGAATTAGTGCGAGAACGGGCATATTTCTTGCTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCAAGCTGCAGCTTGGCCATCGGCTACATACGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATCAAGATACCATGCCAAAACTCAACATATGGATGCAAAATCACAATGAATTTGAACCAGATAAACGAGCACGAGACATCGTGCCCATATGAACCCTGTTCGTGCCCTATATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCAAGACTCAGCCAAAAGCTTCTTATACAATTCAAGGTTCACAATATGCTTGAACAGTGGTGATAGATATCGTATCTTTCAAGCAGAGAACGATGATGCTTTGTTTGTCCTGTCTTACTCCTTTGAAATCTTTGGCAATGCGGTTACTTTGAGCCGGATTGGATCTCCGTTGTGTGAGAAGAAGTTTTGTTATGATATCAAAGCCAAATCACAGGGGAGTGTTCTTTCTTTGCAATCAGTGGCGAATGAGATCCGACGTTGGATTGAAGATCCTCCTTTCAAGGGGTCGCTTTTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGAAGATCAACACTGGGGATCAACACAGGTGCACATACATTTTTTTTGAAACAAAGCTGAATTATTGTTTGAACTGAGTTAATCTTTGCATCTGTGTTTTCTTTGTTTTTTTGGGAAGGACTTGGTGCATACTTTTGTGCATATATATCTATATGGGTTTGTTGAACTTTTAATCTTGTATGGAATAGAACACTAATGTTGAGTGGGTCTATGGCCGATATCGCTTAGTTGTTGGTAGAGCAAATGACTCAAAATCTGCCTTATAGGGAAAGTAAAATATTTATTGAAACAAAGTGGAAGCTAAATTTATAGTTACCTATTT

mRNA sequence

TTTTTAAAATGAACCTTTATTGATACGACCGCAGATAAAGTAGAAAAGAAATGAGAAATATAAAAATGTTTTTTTAAAATCAAATGACGAAACTACCCCTCAATTTCGGAATAAAACTGTTTTTTTCACGACGGAAAGACTTAGATGATCTGCCAAGTCTTCGTCCTCACGGTCTGACGAAGAGCAGAGACTAACTTGAAGAAGAACAAATGGCGGATTCTCGATCATCGGACGGCGAAAAGGAGAGGCGACGTTCGGTCAACTGTAGCAGAGGGAAGAATGAGGCGATGAAAGTCATATTCACTGATCCTCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTACCAGTGCGAGAACGGGCATATTTCTTGCTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCAAGCTGCAGCTTGGCCATCGGCTACATACGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATCAAGATACCATGCCAAAACTCAACATATGGATGCAAAATCACAATGAATTTGAACCAGATAAACGAGCACGAGACATCGTGCCCATATGAACCCTGTTCGTGCCCTATATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCAAGACTCAGCCAAAAGCTTCTTATACAATTCAAGGTTCACAATATGCTTGAACAGTGGTGATAGATATCGTATCTTTCAAGCAGAGAACGATGATGCTTTGTTTGTCCTGTCTTACTCCTTTGAAATCTTTGGCAATGCGGTTACTTTGAGCCGGATTGGATCTCCGTTGTGTGAGAAGAAGTTTTGTTATGATATCAAAGCCAAATCACAGGGGAGTGTTCTTTCTTTGCAATCAGTGGCGAATGAGATCCGACGTTGGATTGAAGATCCTCCTTTCAAGGGGTCGCTTTTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGAAGATCAACACTGGGGATCAACACAGGTGCACATACATTTTTTTTGAAACAAAGCTGAATTATTGTTTGAACTGAGTTAATCTTTGCATCTGTGTTTTCTTTGTTTTTTTGGGAAGGACTTGGTGCATACTTTTGTGCATATATATCTATATGGGTTTGTTGAACTTTTAATCTTGTATGGAATAGAACACTAATGTTGAGTGGGTCTATGGCCGATATCGCTTAGTTGTTGGTAGAGCAAATGACTCAAAATCTGCCTTATAGGGAAAGTAAAATATTTATTGAAACAAAGTGGAAGCTAAATTTATAGTTACCTATTT

Coding sequence (CDS)

ATGGCGGATTCTCGATCATCGGACGGCGAAAAGGAGAGGCGACGTTCGGTCAACTGTAGCAGAGGGAAGAATGAGGCGATGAAAGTCATATTCACTGATCCTCAAATTCTCGATTGCTATATTTGTTGCGAGCCCTTGTCCGTTCCTGTTTACCAGTGCGAGAACGGGCATATTTCTTGCTCTAGCTGCTGCTCCAAAGTTCACAATAAATGCCCAAGCTGCAGCTTGGCCATCGGCTACATACGTTGCAGAGCGATCGAGAAGGTTCTTGAATCAATCAAGATACCATGCCAAAACTCAACATATGGATGCAAAATCACAATGAATTTGAACCAGATAAACGAGCACGAGACATCGTGCCCATATGAACCCTGTTCGTGCCCTATATCTGATTGCTCTTATGTTGATTCGGCAGAGCATTTGGGGTCACACTTCAGAAAAAGGCACCAAGACTCAGCCAAAAGCTTCTTATACAATTCAAGGTTCACAATATGCTTGAACAGTGGTGATAGATATCGTATCTTTCAAGCAGAGAACGATGATGCTTTGTTTGTCCTGTCTTACTCCTTTGAAATCTTTGGCAATGCGGTTACTTTGAGCCGGATTGGATCTCCGTTGTGTGAGAAGAAGTTTTGTTATGATATCAAAGCCAAATCACAGGGGAGTGTTCTTTCTTTGCAATCAGTGGCGAATGAGATCCGACGTTGGATTGAAGATCCTCCTTTCAAGGGGTCGCTTTTGATTCCAAATGAGTTCTTCGGATCTTCTTCAGCCCAGACGATCTTGGAGATTCGCATTTGGCCTTCACGCTGA

Protein sequence

MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAENDDALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIWPSR

Homology

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 5.3e-49
Identity = 108/280 (38.57%), Postives = 153/280 (54.64%), Query Frame = 0


Query: 4   SRSSDGE---KERR-----RSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCEN 63
           S  SD E   +ERR      S + S  K+  + V   DP +LDC ICCEPL +P++QC+N
Sbjct: 70  SDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDN 129

Query: 64  GHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINE 123
           GH++C+ CC+KV N+CPSC+L IGY+RCRA+EKV+E+ ++ C N+ YGCK + +      
Sbjct: 130 GHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFS 189

Query: 124 HETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIF 183
           HE  C + PCSCPI DC Y    + L +H R  H+D   SF++N+R TI L+   +  I 
Sbjct: 190 HEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTIL 249

Query: 184 QAENDDALFVLSYSFEIFGNAVTLS-------RIGSPLCE-KKFCYDIKAKSQGSVLSLQ 243
           Q END  + V+     +    V++S        +G   C   K   D   K    V ++Q
Sbjct: 250 QEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQ 309

Query: 244 SVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIW 268
            V NE        P  G +LIP+  F   S    L ++IW
Sbjct: 310 KVTNE-------HPEDGFMLIPSYLF---SGNDNLNLQIW 339

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 7.9e-37
Identity = 88/269 (32.71%), Postives = 144/269 (53.53%), Query Frame = 0


Query: 4   SRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSC 63
           S S   +K +  S + ++   +    +  D +ILDC IC E  ++P++QC+NGH++CSSC
Sbjct: 16  SNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSC 75

Query: 64  CSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYE 123
           C K++NKCP+C+  +G+ RCRA+E VLESI IPC N+  GCK  ++  +   HE  C + 
Sbjct: 76  CPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFS 135

Query: 124 PCSCPISDCSYVDSAEHLGSHFRKRHQD--SAKSFLYNSRFTICLNSGDRYRIFQAENDD 183
            C+CP  DC+Y  S + L +H+R  H +     +F+ +   ++ +N   +  I      +
Sbjct: 136 HCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTN 195

Query: 184 ALFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE-- 243
            LF +    E +G  VT+S I     E  ++ Y +     G  +  QS   E++R ++  
Sbjct: 196 HLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQS--PEVKRVLKLS 255

Query: 244 -DPPFKGSLLIPNEFFGSSSAQTILEIRI 267
              P +  +LIPN          +LE+RI
Sbjct: 256 FQTPQENFMLIPNSLLRGD----VLEMRI 278

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-35
Identity = 85/255 (33.33%), Postives = 136/255 (53.33%), Query Frame = 0


Query: 4   SRSSDGEKERRRSVNCSRGKNEAMK---VIFTDPQILDCYICCEPLSVPVYQCENGHISC 63
           S  +  +K    S++   G+    K    + TD  ILDC IC + L +PV+QC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 123
           SSCC K+ NKCP+C+L +G+IRCRA+E+VLES+ +PC+ +  GC  T+   + + HE  C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYN--SRFTICLNSGDRYRIFQAE 183
            + PCSCP+  C+Y  S + L  H+   H   + ++ +N  S     +   D+  I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDDALFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKS-QGSVLSLQS-VANEIRR 243
               LFV+    E  G  V++S I     E  +F Y +   + +G  ++ QS    ++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 WIEDPPFKGSLLIPN 251
                P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy Swiss-Prot
Match: Q9FM14 (E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g62800 PE=2 SV=2)

HSP 1 Score: 151.4 bits (381), Expect = 1.5e-35
Identity = 92/270 (34.07%), Postives = 137/270 (50.74%), Query Frame = 0


Query: 1   MADSRS-SDGEKERRRSVN---CSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENG 60
           M DS S    +  +R+S +     R +NE       D  +LDC +C EPL++P +QC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HISCSSCCSKVHNKC--PSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQIN 120
           HI C+ C +KV NKC  P C L IG  RC A+E+VLES  +PCQN+ +GC  +++  +++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 EHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFT----------I 180
            HE  C Y  CSCP  +C+Y  S   +  HF +RH       LYNS             +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRH-------LYNSTIVSSKWGYSTVDV 180

Query: 181 CLNSGDRYRIFQAENDDALFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSV 240
            +N  ++  +        LFV+    E  G  VT+ RI  P  E KKF Y +     G  
Sbjct: 181 LINIKEKVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHN 240

Query: 241 LSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           ++ +S   E++R +E     P    + +PN
Sbjct: 241 VTYES--PEVKRLLEVNSQIPDDSFMFVPN 261

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy Swiss-Prot
Match: Q9FKD5 (Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702 GN=At5g37910 PE=3 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 5.7e-35
Identity = 82/236 (34.75%), Postives = 125/236 (52.97%), Query Frame = 0


Query: 23  KNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLAIGYIR 82
           K +   ++  D  ILDC ICCE L+ P++QC+NGH++C SCC K+ NKCP+C+L +G+ R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 83  CRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLG 142
            RA+E VLESI IPC N  +GC  +    + + HE  C +  CSCP S C Y  S + L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 143 SHFRKRHQD----SAKSFLYNSRFTICLNSGDRYRIFQAENDDALFVLSYSFEIFGNAVT 202
           +H++  H      + K F   + FT  +   D+  I +      L  +    E  G  VT
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 203 LSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           +S I     E  +F Y +     G  ++ +S   E++R  +   + P +  +LIP+
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYES--PEVKRVCKVSIETPQENFMLIPH 255

[Close]

BLAST of Cp4.1LG02g16780 vs. NCBI nr
Match: KAG6606996.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 560 bits (1442), Expect = 2.14e-201
Identity = 270/270 (100.00%), Postives = 270/270 (100.00%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. NCBI nr
Match: XP_022997791.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 548 bits (1411), Expect = 1.14e-196
Identity = 265/270 (98.15%), Postives = 266/270 (98.52%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DALFVLSYSFEIFGNAVTLSRIGSPL EKKFCYDIKAKSQGSVLSLQSVA EI+RW EDP
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. NCBI nr
Match: XP_022949045.1 (uncharacterized protein LOC111452508 [Cucurbita moschata])

HSP 1 Score: 556 bits (1433), Expect = 8.31e-196
Identity = 268/270 (99.26%), Postives = 269/270 (99.63%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRH DSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DA+FVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP
Sbjct: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. NCBI nr
Match: XP_022997790.1 (E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Cucurbita maxima])

HSP 1 Score: 478 bits (1231), Expect = 3.13e-169
Identity = 232/270 (85.93%), Postives = 241/270 (89.26%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MA SRSSDGEKERR SVN SR KNE MKVI TDPQ+LDCYICC PLSVPVYQCENGHISC
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSL IGY+RCRAIEKVLESIK+PCQNS YGCK T NLNQINEHET C
Sbjct: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PY+PCSCP+SDCSYVDSAEHLGSHFRKRHQDSAKS  YNSRFTICLNS D+Y IFQAEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
             LFVLSYSFE FG+AVTL RIG    EKKFCYDIKAKS G+VLSL SVA EI+RW EDP
Sbjct: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           P KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. NCBI nr
Match: KAG6606997.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 477 bits (1228), Expect = 8.97e-169
Identity = 232/270 (85.93%), Postives = 242/270 (89.63%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MA SRSSDGEKERR S N SR KNE MKVI TDPQ+LDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MAGSRSSDGEKERRSSANSSRRKNETMKVILTDPQVLDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKV NKCPSCSLAIGY+RCRAIEKVLESIK+PCQNS YGCK T NLNQINEHET C
Sbjct: 61  SSCCSKVQNKCPSCSLAIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PY+PCSCP+SDCSYVDSAE+L SHFRKRHQDSAKS  YNSRFTICLNS D+Y IFQAEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEYLSSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
             LFVLSYSFE FG+AVTL+RIG    EKKFCYDIKAKSQGSVLSL SVA EI+RW EDP
Sbjct: 181 GVLFVLSYSFETFGDAVTLNRIGPRSSEKKFCYDIKAKSQGSVLSLLSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           P KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy TrEMBL
Match: A0A6J1K8G5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 PE=3 SV=1)

HSP 1 Score: 548 bits (1411), Expect = 5.53e-197
Identity = 265/270 (98.15%), Postives = 266/270 (98.52%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNS YGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DALFVLSYSFEIFGNAVTLSRIGSPL EKKFCYDIKAKSQGSVLSLQSVA EI+RW EDP
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy TrEMBL
Match: A0A6J1GBP1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508 PE=3 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 4.02e-196
Identity = 268/270 (99.26%), Postives = 269/270 (99.63%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PYEPCSCPISDCSYVDSAEHLGSHFRKRH DSAKSFLYNSRFTICLNSGDRYRIFQAEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHPDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
           DA+FVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP
Sbjct: 181 DAVFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           PFKGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy TrEMBL
Match: A0A6J1KAW2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 478 bits (1231), Expect = 1.52e-169
Identity = 232/270 (85.93%), Postives = 241/270 (89.26%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MA SRSSDGEKERR SVN SR KNE MKVI TDPQ+LDCYICC PLSVPVYQCENGHISC
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCCSKVHNKCPSCSL IGY+RCRAIEKVLESIK+PCQNS YGCK T NLNQINEHET C
Sbjct: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
           PY+PCSCP+SDCSYVDSAEHLGSHFRKRHQDSAKS  YNSRFTICLNS D+Y IFQAEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
             LFVLSYSFE FG+AVTL RIG    EKKFCYDIKAKS G+VLSL SVA EI+RW EDP
Sbjct: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           P KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPSR 270

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy TrEMBL
Match: A0A6J1KCJ8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 462 bits (1190), Expect = 7.92e-163
Identity = 232/300 (77.33%), Postives = 241/300 (80.33%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQ-------- 60
           MA SRSSDGEKERR SVN SR KNE MKVI TDPQ+LDCYICC PLSVPVYQ        
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQTVDFQWKL 60

Query: 61  ----------------------CENGHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVL 120
                                 CENGHISCSSCCSKVHNKCPSCSL IGY+RCRAIEKVL
Sbjct: 61  WIMIKIKEICRSFEQLLAKIVECENGHISCSSCCSKVHNKCPSCSLPIGYMRCRAIEKVL 120

Query: 121 ESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHQ 180
           ESIK+PCQNS YGCK T NLNQINEHET CPY+PCSCP+SDCSYVDSAEHLGSHFRKRHQ
Sbjct: 121 ESIKMPCQNSRYGCKTTTNLNQINEHETLCPYQPCSCPLSDCSYVDSAEHLGSHFRKRHQ 180

Query: 181 DSAKSFLYNSRFTICLNSGDRYRIFQAENDDALFVLSYSFEIFGNAVTLSRIGSPLCEKK 240
           DSAKS  YNSRFTICLNS D+Y IFQAEND  LFVLSYSFE FG+AVTL RIG    EKK
Sbjct: 181 DSAKSISYNSRFTICLNSDDKYCIFQAENDGVLFVLSYSFETFGDAVTLKRIGPRSSEKK 240

Query: 241 FCYDIKAKSQGSVLSLQSVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIWPSR 270
           FCYDIKAKS G+VLSL SVA EI+RW EDPP KGSLLIPNEFFGSSSAQTILEIRIWPSR
Sbjct: 241 FCYDIKAKSHGNVLSLLSVAKEIQRWTEDPPSKGSLLIPNEFFGSSSAQTILEIRIWPSR 300

[Close]

BLAST of Cp4.1LG02g16780 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 409 bits (1050), Expect = 5.24e-142
Identity = 198/269 (73.61%), Postives = 228/269 (84.76%), Query Frame = 0


Query: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60
           MADSR+SDGE+ +R  V+  RGK EAM VIF+DPQ+LDCYICCEPLS PV+QCENGHI+C
Sbjct: 1   MADSRASDGERGKRSPVS-GRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60

Query: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 120
           SSCC+KV NKCPSCSL+IGYIRCRAIEKVLESIKIPC NS YGCK+TM LN+ N+H + C
Sbjct: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120

Query: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180
            YEPCSCP+SDC +V S+E L SHF K+H++SAKSF YNSRFT+CLNSGD YRI QAE D
Sbjct: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180

Query: 181 DALFVLSYSFEIFGNAVTLSRIGSPLCEKKFCYDIKAKSQGSVLSLQSVANEIRRWIEDP 240
            ALF LS++FEIFGNAVT+SRIG    EKKFCY+IKAK+QGS+L LQS+A EI+  +E P
Sbjct: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240

Query: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269
           P   SLLIPNEFFGSS AQTILE RIWPS
Sbjct: 241 PSNRSLLIPNEFFGSS-AQTILEFRIWPS 267

[Close]

BLAST of Cp4.1LG02g16780 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 196.1 bits (497), Expect = 3.8e-50
Identity = 108/280 (38.57%), Postives = 153/280 (54.64%), Query Frame = 0


Query: 4   SRSSDGE---KERR-----RSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCEN 63
           S  SD E   +ERR      S + S  K+  + V   DP +LDC ICCEPL +P++QC+N
Sbjct: 70  SDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIPIFQCDN 129

Query: 64  GHISCSSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINE 123
           GH++C+ CC+KV N+CPSC+L IGY+RCRA+EKV+E+ ++ C N+ YGCK + +      
Sbjct: 130 GHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTSYGNRFS 189

Query: 124 HETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIF 183
           HE  C + PCSCPI DC Y    + L +H R  H+D   SF++N+R TI L+   +  I 
Sbjct: 190 HEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLNKKTTIL 249

Query: 184 QAENDDALFVLSYSFEIFGNAVTLS-------RIGSPLCE-KKFCYDIKAKSQGSVLSLQ 243
           Q END  + V+     +    V++S        +G   C   K   D   K    V ++Q
Sbjct: 250 QEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGFMVKNIQ 309

Query: 244 SVANEIRRWIEDPPFKGSLLIPNEFFGSSSAQTILEIRIW 268
            V NE        P  G +LIP+  F   S    L ++IW
Sbjct: 310 KVTNE-------HPEDGFMLIPSYLF---SGNDNLNLQIW 339

[Close]

BLAST of Cp4.1LG02g16780 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 155.6 bits (392), Expect = 5.6e-38
Identity = 88/269 (32.71%), Postives = 144/269 (53.53%), Query Frame = 0


Query: 4   SRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSC 63
           S S   +K +  S + ++   +    +  D +ILDC IC E  ++P++QC+NGH++CSSC
Sbjct: 16  SNSILSQKRQLSSSDAAKRDAKKRSTMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSC 75

Query: 64  CSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYE 123
           C K++NKCP+C+  +G+ RCRA+E VLESI IPC N+  GCK  ++  +   HE  C + 
Sbjct: 76  CPKLNNKCPACTSPVGHNRCRAMESVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFS 135

Query: 124 PCSCPISDCSYVDSAEHLGSHFRKRHQD--SAKSFLYNSRFTICLNSGDRYRIFQAENDD 183
            C+CP  DC+Y  S + L +H+R  H +     +F+ +   ++ +N   +  I      +
Sbjct: 136 HCACPALDCNYTSSYKDLYTHYRITHMEINQINTFICDIPLSVRMNISKKILIRTEHLTN 195

Query: 184 ALFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE-- 243
            LF +    E +G  VT+S I     E  ++ Y +     G  +  QS   E++R ++  
Sbjct: 196 HLFAVQCFREPYGVYVTVSCIAPSSPELSQYSYALSYTVDGHTVIYQS--PEVKRVLKLS 255

Query: 244 -DPPFKGSLLIPNEFFGSSSAQTILEIRI 267
              P +  +LIPN          +LE+RI
Sbjct: 256 FQTPQENFMLIPNSLLRGD----VLEMRI 278

[Close]

BLAST of Cp4.1LG02g16780 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 151.8 bits (382), Expect = 8.1e-37
Identity = 85/255 (33.33%), Postives = 136/255 (53.33%), Query Frame = 0


Query: 4   SRSSDGEKERRRSVNCSRGKNEAMK---VIFTDPQILDCYICCEPLSVPVYQCENGHISC 63
           S  +  +K    S++   G+    K    + TD  ILDC IC + L +PV+QC NGH++C
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 64  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSC 123
           SSCC K+ NKCP+C+L +G+IRCRA+E+VLES+ +PC+ +  GC  T+   + + HE  C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 124 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYN--SRFTICLNSGDRYRIFQAE 183
            + PCSCP+  C+Y  S + L  H+   H   + ++ +N  S     +   D+  I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 184 NDDALFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKS-QGSVLSLQS-VANEIRR 243
               LFV+    E  G  V++S I     E  +F Y +   + +G  ++ QS    ++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQSPKVKKVLK 246

Query: 244 WIEDPPFKGSLLIPN 251
                P    +LIP+
Sbjct: 247 VSSQRPKDSFMLIPH 261

[Close]

BLAST of Cp4.1LG02g16780 vs. TAIR 10
Match: AT5G62800.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-36
Identity = 92/270 (34.07%), Postives = 137/270 (50.74%), Query Frame = 0


Query: 1   MADSRS-SDGEKERRRSVN---CSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENG 60
           M DS S    +  +R+S +     R +NE       D  +LDC +C EPL++P +QC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HISCSSCCSKVHNKC--PSCSLAIGYIRCRAIEKVLESIKIPCQNSTYGCKITMNLNQIN 120
           HI C+ C +KV NKC  P C L IG  RC A+E+VLES  +PCQN+ +GC  +++  +++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 EHETSCPYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFT----------I 180
            HE  C Y  CSCP  +C+Y  S   +  HF +RH       LYNS             +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRH-------LYNSTIVSSKWGYSTVDV 180

Query: 181 CLNSGDRYRIFQAENDDALFVLSYSFEIFGNAVTLSRIGSPLCE-KKFCYDIKAKSQGSV 240
            +N  ++  +        LFV+    E  G  VT+ RI  P  E KKF Y +     G  
Sbjct: 181 LINIKEKVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHN 240

Query: 241 LSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           ++ +S   E++R +E     P    + +PN
Sbjct: 241 VTYES--PEVKRLLEVNSQIPDDSFMFVPN 261

[Close]

BLAST of Cp4.1LG02g16780 vs. TAIR 10
Match: AT5G37910.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 149.4 bits (376), Expect = 4.0e-36
Identity = 82/236 (34.75%), Postives = 125/236 (52.97%), Query Frame = 0


Query: 23  KNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISCSSCCSKVHNKCPSCSLAIGYIR 82
           K +   ++  D  ILDC ICCE L+ P++QC+NGH++C SCC K+ NKCP+C+L +G+ R
Sbjct: 22  KRQRSAIVLLDLDILDCPICCEALTSPIFQCDNGHLACGSCCPKLSNKCPACTLPVGHSR 81

Query: 83  CRAIEKVLESIKIPCQNSTYGCKITMNLNQINEHETSCPYEPCSCPISDCSYVDSAEHLG 142
            RA+E VLESI IPC N  +GC  +    + + HE  C +  CSCP S C Y  S + L 
Sbjct: 82  SRAMESVLESILIPCPNVRFGCTKSFFYGKESAHEKECIFSQCSCPSSVCDYTGSYKDLY 141

Query: 143 SHFRKRHQD----SAKSFLYNSRFTICLNSGDRYRIFQAENDDALFVLSYSFEIFGNAVT 202
           +H++  H      + K F   + FT  +   D+  I +      L  +    E  G  VT
Sbjct: 142 AHYKLTHSTNIFWNIKRFRCANFFTTSMLISDKILIKRVHEKKLLLAVQCFREPCGVYVT 201

Query: 203 LSRIGSPLCE-KKFCYDIKAKSQGSVLSLQSVANEIRRWIE---DPPFKGSLLIPN 251
           +S I     E  +F Y +     G  ++ +S   E++R  +   + P +  +LIP+
Sbjct: 202 VSFIAPSAPEVGEFSYQLSYNVDGHTVTYES--PEVKRVCKVSIETPQENFMLIPH 255

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g16780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84K34	5.3e-49	38.57	E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9FKD7	7.9e-37	32.71	E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3...	[more]
Q9FKD9	1.1e-35	33.33	Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702...	[more]
Q9FM14	1.5e-35	34.07	E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9FKD5	5.7e-35	34.75	Putative E3 ubiquitin-protein ligase SINA-like 9 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Cp4.1LG02g16780 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606996.1	2.14e-201	100.00	E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp....	[more]
XP_022997791.1	1.14e-196	98.15	E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima]	[more]
XP_022949045.1	8.31e-196	99.26	uncharacterized protein LOC111452508 [Cucurbita moschata]	[more]
XP_022997790.1	3.13e-169	85.93	E3 ubiquitin-protein ligase SINA-like 10 isoform X2 [Cucurbita maxima]	[more]
KAG6606997.1	8.97e-169	85.93	E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of Cp4.1LG02g16780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K8G5	5.53e-197	98.15	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 P...	[more]
A0A6J1GBP1	4.02e-196	99.26	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508...	[more]
A0A6J1KAW2	1.52e-169	85.93	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P...	[more]
A0A6J1KCJ8	7.92e-163	77.33	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P...	[more]
A0A6J1CNI1	5.24e-142	73.61	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]

BLAST of Cp4.1LG02g16780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37930.1	3.8e-50	38.57	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37890.1	5.6e-38	32.71	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37870.1	8.1e-37	33.33	Protein with RING/U-box and TRAF-like domains	[more]
AT5G62800.1	1.1e-36	34.07	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37910.1	4.0e-36	34.75	Protein with RING/U-box and TRAF-like domains	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 87..145 e-value: 1.6E-16 score: 62.3
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 81..238 e-value: 9.4E-19 score: 68.2
None	No IPR available	PANTHER	PTHR46632:SF16	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 16..268
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 92..254
IPR044286	E3 ubiquitin-protein ligase SINA-like, plant	PANTHER	PTHR46632	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 16..268
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 92..150 score: 13.65649

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g16780.1	Cp4.1LG02g16780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016740	transferase activity
molecular_function	GO:0008270	zinc ion binding