Cp4.1LG02g16620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g16620
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNicastrin
LocationCp4.1LG02: 12663004 .. 12675062 (-)
RNA-Seq ExpressionCp4.1LG02g16620
SyntenyCp4.1LG02g16620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANTATTTTATTTATTTATTTATTTTTTGAGGGGTTTCCTTTTACCCGTTCTTTCCACTCCCGATGATCAAATTTCAGTTCAATTTCCCAACTCTACGTGGGCTCTCGAGTCCATTGCAGGGACACATTCATCTTCGTCTTGCGCAGAGCTGAGGTCCCCAAGAACGCTCCAACAGACTGCGATTCGAATCAAAGAAGAGCCAAATCAGCATCCATGTCTTTCCATGTTCTTCACCTCCTCCTGTTTTTAACTTCTGTTCGTCTCTGTTTATCAGGTGCGTTAAAAGAGAAGGGCTATCAAATTTTTGTTTCAATGGCTTCTTATTGCTCGTTGACTATGAATGCCAATCAGTCTTGATGCGTTAAAAGTAATGTAACTAATGTGTATGGGTTACTTCCTTACATTTCGTAATCTGAGTAGTCACTAGCAGCTAAAGGGTACATTATTTGAAGTAATTAGATGCCATTTTGCTGCTTGATGTCTAAATTTCACCTCTGAAGGTTTGGTTTGCTCGGATGTTAGTTTTCTGCTTCTTTTCAAGTTCTTATTGCTTATACGAAATTATTTTTAAACTAATAAGATTATATTCTATCCCCGCGAAGCTAATTATCTGGTTCATACGACAATATCACACATGGATGAAGAATAATTATGGGTTTAAGTAAATTTTATCCTTTCTATTATAATTTTACTAAATTTTGGAGTGAGCATTTTGATGTTGACTACAGAGCAAGAATCCTGACCTGTTATCATTTTCTATAATGCATTCAACAGATGAACACTCAATGGAATCGGTTCCTGATCTCCAAAATTCAATGTACCTTGTAGTTGATGGTTATCCGTGCATTCGATTACTCAATCTCTCTGGAGAAATCGGTTGTTCAAGTAGGTTATTTTGCACTATACGTAGAAACTCTATTACCTTTGGAAATGCCAGCGTAATCCTTAATGGTATGGAGGCGTCATTCATGTATTTTTACTATGTGTACCGCAGATCCTGGGCGAGAAAAGGTTGTAGTACCAATGATTAACTTCAAAGATGCTGATGAGATATTGCAACCGTCTGCAATTTTAGTTTCAATGGATGACATCTCGAGTTTCTTTGCTAGGTAGGTTTTAGATTTTTATATATACTCCCTAGCTTCATACATATTCTCATTTCATTATAAGGATTATTTTCTAAACTTGGGTCTACAACATGTTCCAAGCCTCCCAATGAAAAATTATAAATAAGTGAAGAAGCATTCTCATCTACAAAAGAAAAAAAAAGAAAAGAAAAAAAAAAAGGAGAGATTTTGAGTGGAAAAATAACTCGGAAAATCAAGGTCTCCTTTGGGCCTCTTGTACTATTATCTATCACCACTAGCGATTGAATTCATATAAGCAGTCCTCAATTGCTATTTTCCTTGCAAATTATATGAACTGTTTCTCCAGTGGGAAGATATAAATGATCTCTTCCTTCATTTTCTTCTCTGCTTATTAGAGTTGGAAGAAAACTATCTACTCAGGTAGATTTTTTTTTTTTTTGGAAACCTATTTCAGATTACAGGATGATTCCAATTTTGCAAGTAATGTTGGTGGTGTTTTAATCAAACCAGGGACTGAAATACAGAAAAGAACGAAAGGTAGAGTTCATTTATCTATTCAGGTTCTTTTCCCCCTTATTTTTTTCTTTCCTTTTCTTTTTTTGTAGTTGTGTGTAATTCAGACATGCACGTCAATAACTAACGTGTTGATTTATTTTCCTTTGCAGGGTTTTCTCCTGCTCAAAAGTTTCCACAAGCTAAATTTGCTCCTTACCAAAAAATTGACTATGAATGGAACCCAATTGTATGTTTGAATTTTCTTGCTGAGTTTTTTTATTTTTTTTAGGAGTGGTCAACCGTTAGAAGCTTTTTACTTCTGAACTCTTATTTTTTTCTCACTTATAAAGCTAAGCACCAAACTATTGATTGTTGGTTGATATCATATCCGCCATATGATTATTATGAGTTAATCTCAGCCTTCCATATATGAGTAGAAGCTTATAAGTCTTTTGTTAGAAGTATTCAGCTTTTAGCTCCTTGGTGGAGCCTTTTTCACAAGGATTCCTCATGTAACTATAGCCTCTCCATGATTATTAGAATTGGAAGATCTCTTTATAATAGTTTTCACTTTTGGGTGGGGTATTTCTCTATTTTTGTGTGGCTTTTTTATACAAGATATATCTTCTATGAAAAGATAAATAATAATAATAATTAGAAGCTGGTAAGAGTTTTGTCGGCCCATGCAGTCATTAGGGCTGATCTGTGTTTAAATTTGGAGCTACTACTAACTCAAGGGTTTATCTTAACTGAGTCTGAATTCTGATTTTTCTTGAAAATCAGCCTCCATCGAATCACTTTGAAAAGAGGCTGAATTCTGTAATTACTATCTTGGTCTCATTTTTTAAGTTACAATTTCCTCCTGTAGTTCGTCGGGACTAGTTTTGTGAGTTTATCTTTTTTACACCCTATACATTCTTTCATTTTTCTCAATGAGCGGCTTAGTTTCTTATGAAAAAAAAAAAACTTTGTTTTATCACAGGCTTTCATTTCTTCTTTGGCTTGACAATCTGTAGCATCTTACAACTTGCACATGCAGGGATCTGGAGTTATGTGGAATCAATATAACTTTCCTGTTTTCTTAATATCTGAGAGCAGCATTTCCCCTGTACAAGAGGTAATTCTGATTCTTAATCACTCAAAACGTTCAATTGCCTTGAGATATTTCATTTACCATAAGGCTGTTTGGCTCAGTTGTTATATGCTTGTCTCTGGAAATTGATTTTTTATTTTGATTAATCTGATGGTTGCCATCAGACATTCCTTAGTCTTCACATTAGGTAAGATCATAATGTACTTTTATAGTTTTTTTTTAAATGGTTAGCAAAACTGAAATAAAAACAACCCAAAATAAAATGTAGAACCGTTTATGAATAAGTGCATGTGTCATAGGGTTGAAACTCATTGATATTTTGCAATTATGTTTACATTATTTGCTAGCGGTGGGCTTGGGTGGCTACAATTGAATTGAAGAGATATTTTGTTCTTAAAAGATAAGTATGCCAATACTTTTGACCAGAAGAATTTATCTGTTGCACAATCGTACCACTTAATGCAGGCTGCTTCAAAAAATGTGAAGGACAAGAAAGTTTACACGTCTAATGTTGCTGAATTTGATCTGGTGATGCAGGTACTTAGCTTCTGGTGTTACAAATGATTACATTTCAGTGATTTCACATCTTAAATGGAATCACACTTTGCAAAGTTCGTTTAATTCATTTTCTTTTTGTCTAGACAACCAAGGCTGGAACTCATAATTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCGTTAGGTGGATACAGGTTAAGTTTTATTTGATTTTTAGTTTTATTTTTGTCTCCATAGTTTTACAATTTTACTCTGTTTAATTTGTTAAAAGAGATCATTATCTTTACAGTGTCTGGTCGTCGCTTCCTCCAATCAATATTTCTTCAGATAAGTCTAAGCCCATCATTCTCACAGTAGCTTCAATGGACTCTGCGTCTTTCTTCCGTGATAAAAGCATTGGTGCCGACTCCCCCATCTCTGTATGATTTATGTTGAGCAAGTATATTAGTCATAAACCTTACCTGTTTATTCTGCTACCTGTTAAAGTCTTTCTGGTACATTTTAGGGCCTGATTGCATTACTGGCTGCGGTTGATGCACTTTCCCATGTGGATGGACTGGATGATCTTCATAAACAGGTTCTTATAGAAGCTATTTTCTTTGTATCCGATTGTACTGTATTTTTATTAATAGCTATTAACTCAAATGCAAATATCCTATAATCTTTTCATCTACATAATTGCATTTTAGATATAACTGTTATGTCTGACTATGTAGCATTTGAGATATAATTGCTATTTCTTGTTTTTATTGCCTTCTATTGATTTAGTTATTGGATTGTCTTATCTTATTCAATCTTAGTTACAACCTCTCAAAATTAATTTTGAAATGGAGAAAAAAAAAACCCATTTTTCATTCCTACAAAGGACATCAGAAACACAAATTGGTGGAGAAGAAATTACTGTGGTAATAGGTTTCTCGCCAACACAAGGAGTTCTAGCCACAGAAGGCCTACTTGAAAAATTCAGAAATAGACCTTCATCTGATCATTATCCTATTCTACTCTCCGTGGGTTGCTGTAAATGGGGCCCTTCGCCCTTCCGCTTTGAGAATATGTGGCTCCATTTTGTTTGTCAATGGTAGAATATTGGTGCAAGAATACGCCCCTTTGTGGATGATCGGAGACGGTTTCATCAACAAACTTAAAAAACTAAAAGAAGTCTTAAAGAATTGGAACAAGGAGGTTTTCGGTTGTATCTCCACAAAAAGAAACCAATTACTTACTGAAATTGTCCTCCTTGATCAAATGGAGGAAACCGACTCTATTACACTAGTTCAACACACATATAAAAAGCTCATGAAAGCAGAACTTATTTCGTTGGCAGCAAAGGAAGAGCAATCTTAGATAGTAAATGTAAAAAACGATGGTCAGAGGAGGGTGACATAAACCTCTAATTTTTCCATTGTATCATGCAAAGAAACGAAAGAGTATCATTATGGAAATTCTATCCCCATAAAGCAGAAGTTTAGTAAATGAAGATGAAATTGTCTCAGATTTTACACGTCCTTATATACAAAGGACAATGCCCTCTTTGAATTTTCCCACGATCTTGACTGGGACCCTATTGATCAACCGCAAGCTGCCTCCCTCGAGGTTATTTTCACCAAAGAAGACGTGGGGAAGGTGATTCAGCATTCAGGATTCGATAAAACTCCAAGACCGGATGGTTTTACCTCATAATTTTCTAAAAACTGTTGGAACTTTATGAGAGTTGATTTCATGAGAGTATTCCAATATTTTTTTCGAATCGGAGTTATCAATGAAAACTTAAATGAAACATACATTTGTCTGATCTCAAAGAAACTGGATGCCCGCACTGTTGCAGACTTTCGTCCCATAAGCCTTACGACCGGACTATATAAAATCATAGAAAGGATATTATCAGAACATCTTAAAAAGGTTCTCCCTCTCACAATTACCAGGCAACAATCAACTTTTGTAGAGGGGTGACGATGCATCTCTCATGGAAAATGAACTCATAGATGAATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANAACTTTCCTTGAAAACATTCTTGTGACAAAAGGATTTGGCTCAAAATGGCGAAGGTGGATAAAAGGATGCATCTCGTCCACAAACTTCATCATCATCAATGGAAGATCAAGAGGGAAGATTATGCCACACGGGGTCTTCAACAAGGGGATCCACTATCCCATTTACTTGTCATCATGGTAATGGACTGCCTTAGTCGCATACTGACAAAAGCAGAATACGAAGGGCAGATAAAAGGTTTTCAGATTGGTAACGAAGGCTTAAGCATCAACCACCTTCAGTTCGTAGACGATACAATCCTTTTTTCTGACTTGGCAGATATCTCTTCCATAAAAGACGTGATCGAACGGTAAAAACCTTTGAAGGATTTTCTGGACAAAATATCAATCTCCAAAAAACAGAGATAATGGGCATCAATATTAACACATTATTGAAGAATTCGCTAGCATATATGATTGTAAAAATGGAGAATGACCGAATATTTACCTAGGATTACCTCTAATAGGAAACCATAAATATTTTTCCTCTTGAAAGACTATTATTGAAAAAATAGAAAGAAGGCTATCAACATGGATATCATGTTATACTTCAAAAGGTGGAAGACTCACCATAATACAAGCCACATTATCCAACGCCCCCACTTACTACATGTCTCTATTTGAAATGCCACAAAAAGTGGTTTTAGATATAGAAATATTATTGAGAAACTACTTGTAGAAAGATGGCCCACACCTTGTTCGATGGAAGATTATAAACCTCCCAACAGTAAAGGGAGGCCTCAGTCTTTTCTCGATAAAGAAGAAGAACAAAACTCTCCTCACCAAATGGATATGAAGATATCATCACGAAGAAAAGGTGTTGTGGAGAAATCTTATAAAGGCTAACTATACTCCAACATCAAACAAAAATCAATCTCTTCCCTCTTCTGCAAAAGAGCCTTGGAAGTACATAAAGAAACATCAAAACCTCATCACCAACCGAACTTTTCATAGGGTAGGTGATGGAGGAAGCACATCATTTTGGGACCTACCCATGAATTGAAAACACCACGCCCATCAAGGATTTTGTAGAGAAGCATGGTGTTCTCAATTCATGGGTCGGTCCAGAATGATGTGCTCCTTCCATCACCCACCATATGGCGAATTCGATTGGTGATGAGGTTTTGATGTTTCTTTATATACTTTCAAGGCTCTTTTTGTAGATGATGAAGGTGATTGATTTTTGTTTGATGTAGGAGTATATTTAGCCTTTATAAGATTTCTCCTCAATACCTTTTCTTCGTGATGATATCTTCATATCTATTTGGCGAAGAGAGCTTTGTTCTTCTTTATCAAGAAAAGATCGAGGCCTCCCTTTTCTGTTGGGAGGTTTATAATACTCCGTCAAACAAGTTGTGGGCCATCTTTCTACGAGTAGTTTCTAAATAATCTTTCTATGTCTGAAACCACTTTTTGTGGCATTTCAAATAGAGACATGTAGTAAGTGGGGAGGTTGGATAATGTGACTTGTATTAGGGTGAGTCTTGTGCCTTTTGAATTATAACAGGATGACCATGTTGATAGCCTTCTTTCTATTTTTTCAATAATAGTCTTCCAGAAGGAAAAAAATTTGTGGTTTCCTTTTAGAGGTAATCGTAGGTACATATTCGGTCCTTCTCCCTTTTTACAACCATATATGCTAGCACTTTCTTCAATAATCTCTATGCTAATATTGATGCCCATGATCTCTGGTTTTTGGAGATTGATATTTTGTCTAGAGCATCCTTCGAAGGTAGTAAATTTGTTTCAATGAAAGATTTTTGGTTTTAGAATTACAGAGAATAAAGCCCGCTCGTAGTAGTTATTGTTGTTGGTCCTTTTTGTTTTATTTTACACACTTAGTTTAGTGGTTCTATCCCTAAGATGCCCGAATTTATATGCTTATGTATCATTTTTTATATATATGTTTTAGCATTTATGAAATGTATTGGAGGTTTGATGATGATGCGTGGGATATAATCATCCCAAGCTCATATGCAGATTGTCTGTAATTTGTATCCATTCTTTGGCTTCATTCTGATTGAATACATAGATCATGGTGCTTTAAGTCTTGTTTGTATCCTTTGCCTTTTCTTATTATTCAGTTGCTAATAGTTATTCTTGAATATCCAGCTTGTTTTTGTTGTCTTCACTGGAGAGTCTTGGGGCTACCTTGGTAGTAGGAGATTTTTGCTTGAACTTGATTTACAGTCCAATTCTGTCAGTGGCCTTAACAATACATTGATCGATACGGTATATTTCTGAACTTGTAAATTAGTGCTCTGAATTTTAGTTTTCTTGTACTCTTTTCAGTGTAGAATTATAGTCACTCTCTAAGAGAAAAATATATGTTTTCTACTTTTCTTGTTTGAGTGGCTTCCTAGTGCGTTACCATTGGAGAAATACATAATGGATGTAGGGAATCATCAGAAAATACTAAAACAGCTCTTTATGTTTTTGGTGGGTGGATGTTTATCTTTGAAGCTTTTCTCTTTATTTTCAAAATTTTATTTGCTTAATTTGTTCTTTCCAATCGAGAAGATAAATATATTCTTAGTCTAAACCAGTGCAAGAATAGGAAAATTGAAAATGCGTCTACTTATATTGAACAAAGAAAATTCAAGAAGTGCATGGTTTTCAACCATCTGAAACCCTACGTATTATTGATGGTGGCTTGTTGCGGGGATAATTTATGTAAATTGGCAATTGCATGTGGGTGAGGGAAATACACTGACAATAAATTTTCCCCTCATATGAGGTAAGGAAAGTGATGCTACACAGGCTCCTGGTTGAGGAAAATAAGGACTTCATGTAGTCATATTTGAAGTGGTAAAGCATGGCATGCCCTTTGAATGACATGATACAGATTTGACATGATGTATGAAAAGTGATATATATTTTAGCTAGAGCTGCATTTATCTGGTTGTTTGTTGTTTTCCAGTGCGTGTGTGATCTTTCTTTTCTTTTTGATACTTTAGATCCATTTTATTTAAGTTAATGATAAAAAGTTGAACTTATTGTGCTTTTACCTTTTCAATTTAGGTTTTCGAAATTGGCTCTGTTGGAAAGAAATCCAATGATGGATTTGGAAACTTCTTCGCTCACATGACAGAGGTAATGGTTTTTTTTTTCCTCTAAACTGTTTTATTATTATTATTTTAAGGTTTCTCTATACTATGAGACCTCCTATCATGCATACTTACCAAAGGAAGGGTAAAAAGGGAATAGAATGATTTTTCTGTTAGGGTGGGAACCATTAGGATTGTGCGTTTGTGTACGGTCAGTGTGGGTAAGGTTTTTGTAGGGCTTGTGTTGCATGTGGCTGTTATGTTTAATTGGACATTTTATGTCTTCGGAGAGATTTCCAGCTCTCTCGATTAACTGGAGTGTTTGGGTATTGTATTGCTTGTTTTCCCTAGTTGATTGCAATATCTTTGGCTGAAATAACCCATTGGTCATGAACGGTGTTCACTGTGTATTTAAGGTTATTTGTGTTAGATACCTAACATACACATCTGAAATGTTGCTGCAACATTTAAAATCTTGTACTGTATGTTAGGCCTTTTGAGGGAATAATTGAACGTGGAGAATTGCAGAAATATTTTGATTTCCTTAAAATTACTGTCGTAATTGTTTTCTAATGTTTGAGTGAGAGAAGTTTGTAAGGTTAATATAACATCTAAAATATTGAGAAAATAATTCGAAAAACAAAGCAGCAGCTTTTATGGAATTTGAGAATCTTTATGACATTCTTAAACCCTTTAAAAGGATAGCTTGTTCAACTTACCTAGTGATTATTGCAAGCAGCATTGGTTGGTTCTAAAGTGCAGAAGGAAAATTTTATAGAATTGCTGAACACACACGGATTTCATTACCAAAAACAAGAGGGAACGCACGAAGAAAGGCGATATTAGGCATCTCCTCCCAATTTCTCAAAATGGTGATCTATCCAATACATTGATACTTAATAGGAAACGCAAAATGGTTGGCTGTCATTTGCCCAATGAAACCCAATTTTCATTTTGCCTGTGATGGCCAGGAAAGGCCATCTTAGATTCAATGAAAAACATGGTGTTTGATTTTTTTCCCCCTCATTGGTGTTGTCTTGTTGATTAGGCAAAAAATGCAGACTGTTACTACTTAACTGTGTTCTAAATTGATAAACTGTTTATCCGACCTCTTGAGAGTTTGAGACCATTATTTTTCTTCATAGATACTTGATCTATTTTGATATACTAGTTCGATATGGTGCCCTAGTAATGTCATTAGCTATTCTGTTAGTATTTTATTATGCACGGAATAACATAGTCATGTTCGATGAAAGATTTTATTTCAACCTATTATTTTAGCAAGATATTGCTCCTGTAGGTTTCATCTTCCAAGAATGAGACATGGAACGCCTTGAAGCTTGCTCGAGAGTCGCTTCCATTTGAGAACATAAAAGTCTCACCAGCTAGTACTACAAATCCAGGGATACCACCATCTTCGTTGATGGCATTTCTAGCAAAGGTATTTATCAACAACATACTTGATGCAACTATTTTCGTGCTCTCTATTGTTCAAACCTGCTGATTTTTTTATTGAAAAAAAATTAAAATAAGAAACGAATGGCACACAATTCAAGGTTTGAGACTAGAGTTTTGCGAAACAATTTCATTGATGTATAAAATTTATAAAAAGGATAATAATCCATGATGATTACGTAACACTTCTCCAATTGGTTGAAAGGGAAGCATAGCTATATGAAGTAAAGATATTAGACCGTGTACACCAAGAGATAGATAAGTTGACAAGTTTATTTTGGTCCGTACAGAACTCGCAGGTCTCTGGGGTGGTACTAGAAGACTTTGATACTAGCTTTACCAATCAATTTTACCAGAGTCACCTCGACGATTTACGTAAGTTGCATTCTTTTCTTTCCTTCGTTTCCCATTTTGTCGCTCATTGACGATTGTTGTATAGCTGGTCCTATTTTTTCTTACTATTCATATTATTTTTCAATCCAGATAATATAAACTCATCAGCTATTGAAGCAGCTGCTTTACTTGTTGCCCGAACTCTTTACATTCTAGCAACCAACAAAAAGGAATTGAGTAGTTCTGCCCTGAATGCTATCAAATTGAACACCTCGTTGGTTGAAGAGCTTATAGGATGTCTCCTTAACTGTGACCCTGGCCTCTCTTGTGAGTTGGTGAAGAGATATATTTCTCCGACCAATGTCTGTCCAAACCATTATGTTGGTGTTATCCTTGATGAACCATCCTCTACTCCTTATCCTGGTTATGTTCACGATGTTTCAAGATTTGCTTGGAACTTTTTAGCTGACAGAACATCCATCCGTAAAGAGAATACTAGCTCTGTCTGTTCACAGAACTGTGATGACAAAAGTGAGGTGTGCATTGGAGCGGAGACTGGAAAGGGAACTTGTGTTGTATCAACTACCAGGTACCATCATATTTATAGCTAGACACAACTGATTTAGAATGGTGCATTGGTGGACACATGGCATACTTAGTCATTGCATCAAAACTAAAACAAAACAAAACAAAAAAAAACTTAAATTAAGATAACTTAGAGAATATTTAAAACTGCAGGAGGCCTTGAGGAAGGATTGTCCTAGAGAGTATGTTGAAGCCTGTAATGTAGGAAAAAGTTATTTATTGAAGCTGACACAATGTGGCTTTCTCATAATAACTCATGGATTTGAAAATCTTACTAATTTGAGGTAGTTTTGCAGTCTTTCATACATTCAATGGTTCAAAACTGCAAGCTTTGAATAATTTTTCGTAGTATAGAGGTTGTAAGTGGGTTGATTCGACTTGACTAAATTGACGTCTGACTACGAATGGGAAGTCTGGAACGTCTACATTATAGATTATTTGATTAGTTGTCCATGCTCTAATTGTTTTATTATTACTTTTTTTGGGGGGAGTAAAAAACAGGTTCAATATGTAACTACTATGCAAGTTTGATAACAATATTTTAAAGTTTGATTAGTTGCACTGCCTTTCTTTGTTAAATATCTGTAGTTATTTGCAACAGGTACGTTCCAGCATACTCAACACGATTGATGTTCGAATCTGGATCTTGGAATGTGCTTCCTCCGAATTCATCGGACCCAATGGGCGCCGTCGATCCTGTTTGGACAGAGAGCAACTGGAACACCATAGGACTCCGAATGTATACCGTCCAAGCTACTGCTTATGATCGTTTTGTCTTACTTGGAGGCATTACTACTACAATCTTGTCATACTTTGCAATAGTAGCCGTGCGAGGCTCCATTATGAAGGCCTTGAAGAAAGATTAACACAACATTTTAGGCAACTAAATGGAATTAGTAAATGAAAAGGCTGAGAAAGATGGGACACTGTATCAATATAGTTCAATCGTTTTTTCTCCGGAAGATTTTGGATGTAGTTGCAGAGGTGGTGTATTGTTTAATTTGGCAACCCTCTGCCTCCCGTTGGTGAAGGGCGGGATCTGATGCCATTGAAGCTTTGCATGTTAGTACGCAAATGATATGATTCTGTATAGCTTCTTCTGTTTCCCGGTTGTAGTTAATCTTACCTCAGGTGCAGCATCCACACATGGTTGCTTGCCTGATCGTTACCGAAAATCAAACGTCGAGTTGAAGACATTTCTGTATACTTGATTGAACAGTTTATTGGAATTGAAAATAGACAGATAATTGAACTGTTCTTGTAACGGCTCAGGTCCACCGCTAGCAGATATTGTCCTTTTTGGGCTT

mRNA sequence

CATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANTATTTTATTTATTTATTTATTTTTTGAGGGGTTTCCTTTTACCCGTTCTTTCCACTCCCGATGATCAAATTTCAGTTCAATTTCCCAACTCTACGTGGGCTCTCGAGTCCATTGCAGGGACACATTCATCTTCGTCTTGCGCAGAGCTGAGGTCCCCAAGAACGCTCCAACAGACTGCGATTCGAATCAAAGAAGAGCCAAATCAGCATCCATGTCTTTCCATGTTCTTCACCTCCTCCTGTTTTTAACTTCTGTTCGTCTCTGTTTATCAGATGAACACTCAATGGAATCGGTTCCTGATCTCCAAAATTCAATGTACCTTGTAGTTGATGGTTATCCGTGCATTCGATTACTCAATCTCTCTGGAGAAATCGGTTGTTCAAATCCTGGGCGAGAAAAGGTTGTAGTACCAATGATTAACTTCAAAGATGCTGATGAGATATTGCAACCGTCTGCAATTTTAGTTTCAATGGATGACATCTCGAGTTTCTTTGCTAGATTACAGGATGATTCCAATTTTGCAAGTAATGTTGGTGGTGTTTTAATCAAACCAGGGACTGAAATACAGAAAAGAACGAAAGGGTTTTCTCCTGCTCAAAAGTTTCCACAAGCTAAATTTGCTCCTTACCAAAAAATTGACTATGAATGGAACCCAATTGGATCTGGAGTTATGTGGAATCAATATAACTTTCCTGTTTTCTTAATATCTGAGAGCAGCATTTCCCCTGTACAAGAGGCTGCTTCAAAAAATGTGAAGGACAAGAAAGTTTACACGTCTAATGTTGCTGAATTTGATCTGGTGATGCAGACAACCAAGGCTGGAACTCATAATTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCGTTAGGTGGATACAGTGTCTGGTCGTCGCTTCCTCCAATCAATATTTCTTCAGATAAGTCTAAGCCCATCATTCTCACAGTAGCTTCAATGGACTCTGCGTCTTTCTTCCGTGATAAAAGCATTGGTGCCGACTCCCCCATCTCTGGCCTGATTGCATTACTGGCTGCGGTTGATGCACTTTCCCATGTGGATGGACTGGATGATCTTCATAAACAGCTTGTTTTTGTTGTCTTCACTGGAGAGTCTTGGGGCTACCTTGGTAGTAGGAGATTTTTGCTTGAACTTGATTTACAGTCCAATTCTGTCAGTGGCCTTAACAATACATTGATCGATACGGTTTTCGAAATTGGCTCTGTTGGAAAGAAATCCAATGATGGATTTGGAAACTTCTTCGCTCACATGACAGAGGTTTCATCTTCCAAGAATGAGACATGGAACGCCTTGAAGCTTGCTCGAGAGTCGCTTCCATTTGAGAACATAAAAGTCTCACCAGCTAGTACTACAAATCCAGGGATACCACCATCTTCGTTGATGGCATTTCTAGCAAAGAGTCACCTCGACGATTTACATAATATAAACTCATCAGCTATTGAAGCAGCTGCTTTACTTGTTGCCCGAACTCTTTACATTCTAGCAACCAACAAAAAGGAATTGAGTAGTTCTGCCCTGAATGCTATCAAATTGAACACCTCGTTGGTTGAAGAGCTTATAGGATGTCTCCTTAACTGTGACCCTGGCCTCTCTTGTGAGTTGGTGAAGAGATATATTTCTCCGACCAATGTCTGTCCAAACCATTATGTTGGTGTTATCCTTGATGAACCATCCTCTACTCCTTATCCTGGTTATGTTCACGATGTTTCAAGATTTGCTTGGAACTTTTTAGCTGACAGAACATCCATCCGTAAAGAGAATACTAGCTCTGTCTGTTCACAGAACTGTGATGACAAAAGTGAGGTGTGCATTGGAGCGGAGACTGGAAAGGGAACTTGTGTTGTATCAACTACCAGGTACGTTCCAGCATACTCAACACGATTGATGTTCGAATCTGGATCTTGGAATGTGCTTCCTCCGAATTCATCGGACCCAATGGGCGCCGTCGATCCTGTTTGGACAGAGAGCAACTGGAACACCATAGGACTCCGAATGTATACCGTCCAAGCTACTGCTTATGATCGTTTTGTCTTACTTGGAGGCATTACTACTACAATCTTGTCATACTTTGCAATAGTAGCCGTGCGAGGCTCCATTATGAAGGCCTTGAAGAAAGATTAACACAACATTTTAGGCAACTAAATGGAATTAGTAAATGAAAAGGCTGAGAAAGATGGGACACTGTATCAATATAGTTCAATCGTTTTTTCTCCGGAAGATTTTGGATGTAGTTGCAGAGGTGGTGTATTGTTTAATTTGGCAACCCTCTGCCTCCCGTTGGTGAAGGGCGGGATCTGATGCCATTGAAGCTTTGCATGTTAGTACGCAAATGATATGATTCTGTATAGCTTCTTCTGTTTCCCGGTTGTAGTTAATCTTACCTCAGGTGCAGCATCCACACATGGTTGCTTGCCTGATCGTTACCGAAAATCAAACGTCGAGTTGAAGACATTTCTGTATACTTGATTGAACAGTTTATTGGAATTGAAAATAGACAGATAATTGAACTGTTCTTGTAACGGCTCAGGTCCACCGCTAGCAGATATTGTCCTTTTTGGGCTT

Coding sequence (CDS)

ATGTCTTTCCATGTTCTTCACCTCCTCCTGTTTTTAACTTCTGTTCGTCTCTGTTTATCAGATGAACACTCAATGGAATCGGTTCCTGATCTCCAAAATTCAATGTACCTTGTAGTTGATGGTTATCCGTGCATTCGATTACTCAATCTCTCTGGAGAAATCGGTTGTTCAAATCCTGGGCGAGAAAAGGTTGTAGTACCAATGATTAACTTCAAAGATGCTGATGAGATATTGCAACCGTCTGCAATTTTAGTTTCAATGGATGACATCTCGAGTTTCTTTGCTAGATTACAGGATGATTCCAATTTTGCAAGTAATGTTGGTGGTGTTTTAATCAAACCAGGGACTGAAATACAGAAAAGAACGAAAGGGTTTTCTCCTGCTCAAAAGTTTCCACAAGCTAAATTTGCTCCTTACCAAAAAATTGACTATGAATGGAACCCAATTGGATCTGGAGTTATGTGGAATCAATATAACTTTCCTGTTTTCTTAATATCTGAGAGCAGCATTTCCCCTGTACAAGAGGCTGCTTCAAAAAATGTGAAGGACAAGAAAGTTTACACGTCTAATGTTGCTGAATTTGATCTGGTGATGCAGACAACCAAGGCTGGAACTCATAATTCAATGTCTTGTTTGAAAGAAGAAACATGCCTTCCGTTAGGTGGATACAGTGTCTGGTCGTCGCTTCCTCCAATCAATATTTCTTCAGATAAGTCTAAGCCCATCATTCTCACAGTAGCTTCAATGGACTCTGCGTCTTTCTTCCGTGATAAAAGCATTGGTGCCGACTCCCCCATCTCTGGCCTGATTGCATTACTGGCTGCGGTTGATGCACTTTCCCATGTGGATGGACTGGATGATCTTCATAAACAGCTTGTTTTTGTTGTCTTCACTGGAGAGTCTTGGGGCTACCTTGGTAGTAGGAGATTTTTGCTTGAACTTGATTTACAGTCCAATTCTGTCAGTGGCCTTAACAATACATTGATCGATACGGTTTTCGAAATTGGCTCTGTTGGAAAGAAATCCAATGATGGATTTGGAAACTTCTTCGCTCACATGACAGAGGTTTCATCTTCCAAGAATGAGACATGGAACGCCTTGAAGCTTGCTCGAGAGTCGCTTCCATTTGAGAACATAAAAGTCTCACCAGCTAGTACTACAAATCCAGGGATACCACCATCTTCGTTGATGGCATTTCTAGCAAAGAGTCACCTCGACGATTTACATAATATAAACTCATCAGCTATTGAAGCAGCTGCTTTACTTGTTGCCCGAACTCTTTACATTCTAGCAACCAACAAAAAGGAATTGAGTAGTTCTGCCCTGAATGCTATCAAATTGAACACCTCGTTGGTTGAAGAGCTTATAGGATGTCTCCTTAACTGTGACCCTGGCCTCTCTTGTGAGTTGGTGAAGAGATATATTTCTCCGACCAATGTCTGTCCAAACCATTATGTTGGTGTTATCCTTGATGAACCATCCTCTACTCCTTATCCTGGTTATGTTCACGATGTTTCAAGATTTGCTTGGAACTTTTTAGCTGACAGAACATCCATCCGTAAAGAGAATACTAGCTCTGTCTGTTCACAGAACTGTGATGACAAAAGTGAGGTGTGCATTGGAGCGGAGACTGGAAAGGGAACTTGTGTTGTATCAACTACCAGGTACGTTCCAGCATACTCAACACGATTGATGTTCGAATCTGGATCTTGGAATGTGCTTCCTCCGAATTCATCGGACCCAATGGGCGCCGTCGATCCTGTTTGGACAGAGAGCAACTGGAACACCATAGGACTCCGAATGTATACCGTCCAAGCTACTGCTTATGATCGTTTTGTCTTACTTGGAGGCATTACTACTACAATCTTGTCATACTTTGCAATAGTAGCCGTGCGAGGCTCCATTATGAAGGCCTTGAAGAAAGATTAA

Protein sequence

MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKKD
Homology
BLAST of Cp4.1LG02g16620 vs. ExPASy Swiss-Prot
Match: Q8GUM5 (Nicastrin OS=Arabidopsis thaliana OX=3702 GN=At3g52640/At3g52650 PE=2 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.9e-231
Identity = 407/661 (61.57%), Postives = 502/661 (75.95%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGTHNS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL S++V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK----------------------S 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ K                      S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGCLLNCD 487
           HLDDL NINSS++ AAA +VARTLYILA++ K+ S+SAL +I +N S VEEL+ CLL C+
Sbjct: 436 HLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEELLTCLLACE 495

Query: 488 PGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTSIRKEN 547
           PGLSC LVK YISPTN CP +Y GVIL EPSS PY GYV DVSRF WNFLAD+TS++K N
Sbjct: 496 PGLSCNLVKDYISPTNTCPGNYAGVILGEPSSKPYLGYVGDVSRFLWNFLADKTSVQKGN 555

Query: 548 TSSVCSQN-CDDKSEVCIGAETGK-GTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDP 607
           T+SVCS+  C    EVCI AE+ K GTCVVSTTRYVPAYSTRL +  G+W +LP NSSD 
Sbjct: 556 TTSVCSKGVCSKTDEVCIKAESNKEGTCVVSTTRYVPAYSTRLKYNDGAWTILPQNSSDS 615

Query: 608 MGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKK 643
           MG VDPVWTESNW+T+ + +YTVQ +AYD  VL+ GIT T L+Y  I+A +  I KALK+
Sbjct: 616 MGMVDPVWTESNWDTLRVHVYTVQHSAYDNAVLVAGITVTTLAYIGILAAKSIITKALKQ 675

BLAST of Cp4.1LG02g16620 vs. ExPASy Swiss-Prot
Match: Q54JT7 (Nicastrin OS=Dictyostelium discoideum OX=44689 GN=ncstn PE=3 SV=2)

HSP 1 Score: 179.5 bits (454), Expect = 1.2e-43
Identity = 174/679 (25.63%), Postives = 299/679 (44.04%), Query Frame = 0

Query: 25  MESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP-GREKVVVPMINFKDADEIL----- 84
           + S   +++ MY  ++ YPC R++ L+G+IGCS+  G +  ++ +I   D+DE       
Sbjct: 21  ISSQSSIEDKMYTSLNSYPCTRIMTLNGQIGCSSSHGGDSGILYLI---DSDESYHNYFS 80

Query: 85  --QPSAILVSMDDISSFFAR-LQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAK 144
             Q   I+V  D  S++F + L  +      + G L+   T+I K T  +SP  ++P  +
Sbjct: 81  YNQQKDIIVVFD--SNYFNKTLVLEMYSKKKMNGALVL--TDIGK-TYPYSPEDQYPIKQ 140

Query: 145 FAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKVYTSNVAEFD 204
           F  Y   +  WNP G G  +  + FP+F +   +   ++  ++ N +D K Y +  AE D
Sbjct: 141 FGLYPDSNLNWNPNGDGFTYMNFPFPMFALELKTSIIIRNLSTIN-RDGK-YPAYGAELD 200

Query: 205 LVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKPIILTVASMDSASFF 264
             MQ    G  N+ +CL+   C P+GG S+WSS   +    D+SKPIIL +  +D+ +FF
Sbjct: 201 SFMQ----GAINAETCLRRGFCEPVGGQSIWSSFSEV---IDQSKPIILVMLPIDATAFF 260

Query: 265 RDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRFLLEL- 324
           RD + G D     L  LL+ ++ L  VD      K+++F ++  E WGY+GS  F+ +L 
Sbjct: 261 RDLATGTDQSGYALTVLLSMLNTLQGVD-KTKWDKEVIFAMWNSERWGYVGSTNFVNDLL 320

Query: 325 -----DLQSNSVSGLNN-TLIDTVF------------EIGSVGKKSNDG--------FGN 384
                 L SN+ +  ++  ++D  F            E   +G+  N G          N
Sbjct: 321 NFNCTSLDSNNQNSCSSPPMLDLTFEQIKFENIYAIIEFNQIGRPVNSGKKTPNKLDIYN 380

Query: 385 FFAHMTEVSSSKNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKSHLDDL 444
              H     +  N+  +    + +S  +EN  +    TT   +PP S M+F+ + +    
Sbjct: 381 LVFH-PNGGAGANQLMDVFSQSTQS--YENSTIQFQKTTQNELPPCSSMSFIKEINKKSA 440

Query: 445 HNI-----------------------NSSAIEAAALLVARTLYILATNKKELSSSALNAI 504
            N                        NS  I      +   + +  +   +L +     +
Sbjct: 441 PNFIGTLVITDHDYQYNNPYFGDEQDNSGNINTTTSTLFDMVQVF-SKSIDLLAGGNGTV 500

Query: 505 KLNTSLVEELIGCLLNCDPGLSCELVKRYIS--PTNVCPNHYVGVILDEPSSTPYPGYVH 564
           K++   + E+  CL      ++C  V + +S  P N  PN Y GV    P +   P    
Sbjct: 501 KVDDLFIREINVCLTQ---SITCNWVTKLMSTFPYNPIPNFYSGVYGVSPVNHITP---- 560

Query: 565 DVSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTR 624
             +RF +      T  R   T+     +CD  S +C+        C+ S T Y  A S  
Sbjct: 561 IETRFIFRMATYLTQHRTNATNCTSDNDCDTSSSICVNK-----VCLYSNTHYHNAISLA 620

Query: 625 LMFES--GSWNVLPPNSSDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTT 641
             F++   SW ++  N+S       PV+ ESNW+   +R++ V + A + + L+ G+   
Sbjct: 621 FSFDNSKSSWTIV--NTS------YPVFVESNWDYTTVRLFQVGSYANEIWFLVSGLIEL 657

BLAST of Cp4.1LG02g16620 vs. ExPASy Swiss-Prot
Match: F0ZBA6 (Nicastrin OS=Dictyostelium purpureum OX=5786 GN=ncstn PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 5.1e-42
Identity = 168/681 (24.67%), Postives = 297/681 (43.61%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCS--- 60
           M F  + +LL L    +  S+  +  ++ D  N    +   YPC +++   G+ GCS   
Sbjct: 1   MRFKNVLVLLLLLVFSVINSEPSAPATISD--NIYTTLYSSYPCTKIMTSDGQFGCSSKH 60

Query: 61  --NPGREKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPG 120
             N G   ++    ++ +     Q   I+V + D + F +    + +  S + G+++   
Sbjct: 61  GGNSGILYLIDDDESYNNYFSYSQQKDIIVVL-DTNYFNSTSVLNLHNKSKIEGIIVLTD 120

Query: 121 TEIQKRTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQE 180
           T   K+T  +SP  ++P   +  Y   + EWNP   G  +  + FP+F I   +   ++ 
Sbjct: 121 T---KKTYPYSPDSRYPNKIYGLYPNSNLEWNPNADGFTYFSFPFPIFAIDNQTSVAIRN 180

Query: 181 AASKNVKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINIS 240
             SK+ +D + Y +  AE D  MQ    G  NS +CL+   C P+GG S+WSS    +  
Sbjct: 181 -VSKHNRDGQ-YPAWGAELDSFMQ----GAINSETCLRRGFCEPVGGQSIWSS---FSSK 240

Query: 241 SDKSKPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFV 300
            DK K IIL +   D+ +FFRD SIGAD      + LL+ + +L+ VD     +K++VF 
Sbjct: 241 IDKEKEIILVMLPFDTTAFFRDLSIGADQSSFATVTLLSVIKSLAAVD-RSSWNKEVVFA 300

Query: 301 VFTGESWGYLGSRRFLLE-LDLQSNSVSG------------------LNNTLIDTVFEIG 360
            +  E WGY+GS  F+ + L+ Q  + +                   +N T I T+ E+ 
Sbjct: 301 FWNAERWGYVGSEYFINDLLNFQCKTYNSDKSKCIDPPRADLAFQTQINFTKISTIIELN 360

Query: 361 SVGKKSND-GFGNFFAHMTEVSSSKNETWNALKLARESLPFENIKVSPASTTNPGIPPSS 420
            +G+   D   G +  ++    +  +   + L     S  +EN  ++   TT   +PPSS
Sbjct: 361 QIGRAQLDKNLGKYSLYLHTAGTKTSSVTDILDQVASS--YENSTITFKPTTQTELPPSS 420

Query: 421 LMAFLAKSH----------------------LDDLHNINSSAIEAAALLVARTLYILATN 480
            M+FL K++                       DD  N+  S +          +YIL+T 
Sbjct: 421 SMSFLKKTNKIPVVVITDHDYKYSNPYYGYEQDDNENVLGSTLN-------DIVYILSTF 480

Query: 481 KKELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEP 540
              ++    N I ++ + +  L  C  +    ++C  +     P N  PN Y  V     
Sbjct: 481 IDRIAGGN-NNITIDKNFINILYPCFTS---SITCFNILMKTYPLNEVPNFYSSVFGTSL 540

Query: 541 SSTPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVST 600
           ++T  P     + R  ++        +  +T + C+ + D  S +C       G CV S 
Sbjct: 541 TTTLSPYETKLIHRLLYSI------TQYNSTLTNCTSDNDCPSSLCY-----SGQCVSSN 600

Query: 601 TRYVPAYSTRLMFESGS--WNVLPPNSSDPMGAVDPVWTESNWNTIGLRMYTVQATAYDR 633
           T    A S    F++    W ++  NSS       P++TESNW+   L+++ +  +  + 
Sbjct: 601 THLHNALSLGFDFDTSKNVWKIV--NSS------YPIFTESNWDYTALKVFKIGNSTTEI 633

BLAST of Cp4.1LG02g16620 vs. ExPASy Swiss-Prot
Match: Q92542 (Nicastrin OS=Homo sapiens OX=9606 GN=NCSTN PE=1 SV=2)

HSP 1 Score: 110.2 bits (274), Expect = 9.1e-23
Identity = 150/665 (22.56%), Postives = 258/665 (38.80%), Query Frame = 0

Query: 43  PCIRLLNLSGEIGC--SNPGREKVVVPMINFKDADEILQPS------AILVSMDDISSFF 102
           PC+RLLN + +IGC  S  G   V+  +   +D   +L          +L S        
Sbjct: 49  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLESKHFTRDLM 108

Query: 103 ARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKI---------DYE 162
            +L+  ++  + +   L KP         GFSP+ + P   F  Y            + +
Sbjct: 109 EKLKGRTSRIAGLAVSLTKPSP-----ASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQ 168

Query: 163 WNPIGSGVMWNQYNFPVFLISES-------------SISPVQEAASKNVKDKKVYTSNVA 222
           WN +G+G+ +  ++FP+FL+ +              ++S    A +  +   ++++   A
Sbjct: 169 WNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLSQNGSAPTFPLCAMQLFSHMHA 228

Query: 223 EFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKP---IILTVASM 282
                    ++   ++ S   E  C PL  Y+VWS L PIN ++   KP   +++    +
Sbjct: 229 VISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPIN-TTGTLKPDDRVVVAATRL 288

Query: 283 DSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRR 342
           DS SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R
Sbjct: 289 DSRSFFWNVAPGAESAVASFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSR 348

Query: 343 FLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWNALKL 402
            + +++     V  L N  +D+  E+G V  +++      + H   VS       N ++ 
Sbjct: 349 MVYDMEKGKFPVQ-LEN--VDSFVELGQVALRTS---LELWMHTDPVSQKNESVRNQVED 408

Query: 403 ARESLPFENIKVSPASTTNPG----IPPSSLMAFLA---------------------KSH 462
              +L      V       P     +PPSSL  FL                      +S 
Sbjct: 409 LLATLEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSI 468

Query: 463 LDDLHNINSS-------------------AIEAAALLVARTLYILATNKKELSSSALNAI 522
            D   NIN S                   A+   A ++ R LY LA       +   +  
Sbjct: 469 YDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYELAGGTNFSDTVQADPQ 528

Query: 523 KLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDV 582
            +   L   LI    +    +  + ++ Y+    +   HY+ V    P++T Y   V   
Sbjct: 529 TVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPL--QHYIAV--SSPTNTTY--VVQYA 588

Query: 583 SRFAWNFLADRTSIRKENTSSVCSQNCDDKSEVCI-----GAETGK-GTCVVSTTRYVPA 625
                  + + T  + ++ S V S+N D      +       ET +   CV ST R   A
Sbjct: 589 LANLTGTVVNLTREQCQDPSKVPSENKDLYEYSWVQGPLHSNETDRLPRCVRSTARLARA 648

BLAST of Cp4.1LG02g16620 vs. ExPASy Swiss-Prot
Match: Q8CGU6 (Nicastrin OS=Rattus norvegicus OX=10116 GN=Ncstn PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 1.2e-22
Identity = 154/675 (22.81%), Postives = 274/675 (40.59%), Query Frame = 0

Query: 43  PCIRLLNLSGEIGC-SNPGREKVVVPMINFKD------ADEILQPSAILVSMDDIS-SFF 102
           PC+RLLN + +IGC S+   +  V+ ++  +D       D    P  +L+     +    
Sbjct: 48  PCVRLLNATHQIGCQSSISGDTGVIHVVEKEDDLKWVLTDGPNPPYMVLLEGKLFTRDIM 107

Query: 103 ARLQDDSNFASNVGGVLIKPGTEIQKRTKGFSPAQKFPQAKFAPYQKI---------DYE 162
            +L+ +++  + +   L KP +     T  FSP+ + P   F  Y               
Sbjct: 108 EKLKGETSRIAGLAVTLAKPNS-----TSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTL 167

Query: 163 WNPIGSGVMWNQYNFPVFLISESSISPV-------------QEAASKNVKDKKVYTSNVA 222
           WN +G+G+ ++ ++FP+FL+ + + + V               A S  +   ++++   A
Sbjct: 168 WNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHA 227

Query: 223 EFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSKPIILTVAS--MD 282
                    ++   ++ S   E  C PL  Y+VWS L PIN S      + + VA+  +D
Sbjct: 228 VISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATRLD 287

Query: 283 SASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYLGSRRF 342
           S SFF + + GA+S ++  +  LAA +AL     +  L + ++FV F GE++ Y+GS R 
Sbjct: 288 SRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRM 347

Query: 343 LLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS-KNETWNALKL 402
           + +++   N    +    ID+  E+G V  +++         M++ + S KN+  + L  
Sbjct: 348 VYDME---NGKFPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVT 407

Query: 403 ARESLPFENIKVSPASTTNPGIPPSSLMAFLA---------------------KSHLDDL 462
             +S       V      +  +PPSSL  FL                      +S  D  
Sbjct: 408 LEQSGADTPQVVLSRLVQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTA 467

Query: 463 HNIN-------------------SSAIEAAALLVARTLYILATNKKELSSSALNAIKLNT 522
            NIN                   + A+   A ++AR LY LA       ++  N+I+ + 
Sbjct: 468 ENINVTYPESQSPEEDLNFVTDTAKALADVATVLARALYKLAG-----GTNFNNSIQADP 527

Query: 523 SLVEELI-GCLLNCDPGLSCELVKRYI-SPTNVCP-NHYVGVILDEPSSTPYPGYVHDVS 582
             V  L+ G L+  +      +++  + S  +  P  HY+ V    P++T Y      V 
Sbjct: 528 QTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAV--SSPTNTTY------VV 587

Query: 583 RFAWNFLADRTSIRKENTSSVCSQNCDDKSEV------------CIGAETGKGT-----C 625
           ++A   LA+ T       +++  + C D S+V              G      T     C
Sbjct: 588 QYA---LANLTG----KVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRC 647

BLAST of Cp4.1LG02g16620 vs. NCBI nr
Match: XP_023525268.1 (nicastrin [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1263 bits (3269), Expect = 0.0
Identity = 642/664 (96.69%), Postives = 642/664 (96.69%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK------------------ 420
           NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK                  
Sbjct: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 ----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
               SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 642
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

BLAST of Cp4.1LG02g16620 vs. NCBI nr
Match: KAG7036691.1 (Nicastrin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1253 bits (3243), Expect = 0.0
Identity = 636/664 (95.78%), Postives = 640/664 (96.39%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFH+LHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGT NSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQS+SVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK------------------ 420
           NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK                  
Sbjct: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 ----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
               SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRL F+SGSWNVLPPN+
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 642
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

BLAST of Cp4.1LG02g16620 vs. NCBI nr
Match: XP_022948878.1 (nicastrin [Cucurbita moschata])

HSP 1 Score: 1248 bits (3230), Expect = 0.0
Identity = 632/664 (95.18%), Postives = 640/664 (96.39%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFH+LHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDG+PCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGHPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKK+YTSNVAEFDLVMQTTKAGT NSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLA+VDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLASVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQS+SVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK------------------ 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAK                  
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 ----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
               SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRL F+SGSWNVLPPN+
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 642
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

BLAST of Cp4.1LG02g16620 vs. NCBI nr
Match: KAG6606989.1 (Nicastrin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1248 bits (3228), Expect = 0.0
Identity = 633/664 (95.33%), Postives = 639/664 (96.23%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFH+LHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 46  MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 105

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 106 REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 165

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 166 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 225

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKK+YTSNVAEFDLVMQTTKAGT NSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 226 VKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 285

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 286 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 345

Query: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQS+SVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 346 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 405

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK------------------ 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAK                  
Sbjct: 406 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 465

Query: 421 ----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
               SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 466 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 525

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 526 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 585

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRL F+SGSWNVLP N+
Sbjct: 586 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPLNA 645

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 642
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 646 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 705

BLAST of Cp4.1LG02g16620 vs. NCBI nr
Match: XP_022997757.1 (nicastrin [Cucurbita maxima])

HSP 1 Score: 1243 bits (3217), Expect = 0.0
Identity = 630/664 (94.88%), Postives = 635/664 (95.63%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           M+FH+LHLLLFLTS RLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MAFHILHLLLFLTSARLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEI QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEIFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINIS DKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISLDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQS+SVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK------------------ 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAK                  
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 ----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
               SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEE+IGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISP NVCPNHYVGVILDEPSSTPYPGYVHDVSRF WNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600
           I KENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS
Sbjct: 541 IPKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 642
           SDPMGAVDPVWTESNWNTIGLR YTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

BLAST of Cp4.1LG02g16620 vs. ExPASy TrEMBL
Match: A0A6J1GAI9 (Nicastrin OS=Cucurbita moschata OX=3662 GN=LOC111452408 PE=3 SV=1)

HSP 1 Score: 1248 bits (3230), Expect = 0.0
Identity = 632/664 (95.18%), Postives = 640/664 (96.39%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MSFH+LHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDG+PCIRLLNLSGEIGCSNPG
Sbjct: 1   MSFHILHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGHPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKK+YTSNVAEFDLVMQTTKAGT NSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK
Sbjct: 181 VKDKKIYTSNVAEFDLVMQTTKAGTRNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLA+VDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLASVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQS+SVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK------------------ 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAK                  
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 ----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
               SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600
           IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRL F+SGSWNVLPPN+
Sbjct: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLTFDSGSWNVLPPNA 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 642
           SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

BLAST of Cp4.1LG02g16620 vs. ExPASy TrEMBL
Match: A0A6J1K5Z2 (Nicastrin OS=Cucurbita maxima OX=3661 GN=LOC111492619 PE=3 SV=1)

HSP 1 Score: 1243 bits (3217), Expect = 0.0
Identity = 630/664 (94.88%), Postives = 635/664 (95.63%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           M+FH+LHLLLFLTS RLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG
Sbjct: 1   MAFHILHLLLFLTSARLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVVVPMINFKDADEI QPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK
Sbjct: 61  REKVVVPMINFKDADEIFQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN
Sbjct: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKSK 240
           VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINIS DKSK
Sbjct: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISLDKSK 240

Query: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300
           PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE
Sbjct: 241 PIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGE 300

Query: 301 SWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360
           SWGYLGSRRFLLELDLQS+SVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK
Sbjct: 301 SWGYLGSRRFLLELDLQSDSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSK 360

Query: 361 NETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK------------------ 420
           NETWNALKLA+ESLPFENIKVSPASTTNPGIPPSSLMAFLAK                  
Sbjct: 361 NETWNALKLAQESLPFENIKVSPASTTNPGIPPSSLMAFLAKNSQVSGVVLEDFDTSFTN 420

Query: 421 ----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGC 480
               SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEE+IGC
Sbjct: 421 QFYQSHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEEIIGC 480

Query: 481 LLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTS 540
           LLNCDPGLSCELVKRYISP NVCPNHYVGVILDEPSSTPYPGYVHDVSRF WNFLADRTS
Sbjct: 481 LLNCDPGLSCELVKRYISPINVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRTS 540

Query: 541 IRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600
           I KENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS
Sbjct: 541 IPKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPNS 600

Query: 601 SDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 642
           SDPMGAVDPVWTESNWNTIGLR YTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA
Sbjct: 601 SDPMGAVDPVWTESNWNTIGLRTYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKA 660

BLAST of Cp4.1LG02g16620 vs. ExPASy TrEMBL
Match: A0A6J1CNM4 (Nicastrin OS=Momordica charantia OX=3673 GN=LOC111013258 PE=3 SV=1)

HSP 1 Score: 1137 bits (2941), Expect = 0.0
Identity = 571/665 (85.86%), Postives = 613/665 (92.18%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP 60
           MS ++L+LL+FLTS+RL LSD+ HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP
Sbjct: 1   MSSNILYLLVFLTSLRLSLSDDTHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNP 60

Query: 61  GREKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQ 120
           GREKVVVPMINFKDADE+ QPSAI+VSMD+ISSFF+RL+DDSNFA+NVGGVLI+PGTE+Q
Sbjct: 61  GREKVVVPMINFKDADELSQPSAIVVSMDEISSFFSRLKDDSNFANNVGGVLIEPGTEMQ 120

Query: 121 KRTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASK 180
            RT+GFSPA+KFPQA+FAPYQK+DY+WNPIGSG+MW QYNFPVFLISE+SIS + EA+SK
Sbjct: 121 NRTEGFSPARKFPQAEFAPYQKVDYDWNPIGSGIMWKQYNFPVFLISETSISSIHEASSK 180

Query: 181 NVKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS 240
           NVK+KK YTSNVAEFDLVMQTTKAGTHNS+SCLKEETCLPLGGYSVWSSLPPINISSD+S
Sbjct: 181 NVKNKKAYTSNVAEFDLVMQTTKAGTHNSVSCLKEETCLPLGGYSVWSSLPPINISSDQS 240

Query: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 300
           KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVF VFTG
Sbjct: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFAVFTG 300

Query: 301 ESWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS 360
           ESWGYLGSRRFLLELDLQS+ VSGLNN LIDTVFEIGSVGK S+ G GNFFAHMTEVSSS
Sbjct: 301 ESWGYLGSRRFLLELDLQSDVVSGLNNKLIDTVFEIGSVGKSSSHGIGNFFAHMTEVSSS 360

Query: 361 KNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK----------------- 420
           KNETWNALK A+ESLPFE  K+SPASTTNPGIPPSSLMAFL K                 
Sbjct: 361 KNETWNALKHAQESLPFEITKISPASTTNPGIPPSSLMAFLKKNSHVSGVVLEDFDTGFT 420

Query: 421 -----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIG 480
                SHLDDL+NINSSAIEAAALLVARTLYILATNKKEL SSA+ +IK+NTSLVEELIG
Sbjct: 421 NQFYQSHLDDLYNINSSAIEAAALLVARTLYILATNKKELISSAITSIKVNTSLVEELIG 480

Query: 481 CLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRT 540
           CLLNCDPGLSCELVKRYISPT+VCPNHYVGVILDEPSSTPYPGYVHDVSRF WNFLADRT
Sbjct: 481 CLLNCDPGLSCELVKRYISPTSVCPNHYVGVILDEPSSTPYPGYVHDVSRFVWNFLADRT 540

Query: 541 SIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPN 600
           SI KENTSS CS NC+DKSEVCIGAE GKGTCV+STTRYVPAYSTRL +ESGSW VLP N
Sbjct: 541 SISKENTSSACSPNCNDKSEVCIGAEIGKGTCVISTTRYVPAYSTRLKYESGSWYVLPSN 600

Query: 601 SSDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMK 642
           SSDPMGAVDPVWTESNWNTIGLRMYT+Q TAYDRFVLLGGITTTIL+YFAIVAVRGSI+K
Sbjct: 601 SSDPMGAVDPVWTESNWNTIGLRMYTIQTTAYDRFVLLGGITTTILAYFAIVAVRGSIIK 660

BLAST of Cp4.1LG02g16620 vs. ExPASy TrEMBL
Match: A0A1S3C4C7 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 1129 bits (2920), Expect = 0.0
Identity = 572/665 (86.02%), Postives = 605/665 (90.98%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MS   L+LLLFLTS+RL  SDE  MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNPG
Sbjct: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVV+PMINFKDADEIL+PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ 
Sbjct: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RT+GFSPAQKFPQAKFAPY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKN
Sbjct: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISS-DKS 240
           VK KK Y SNVAEFDLVMQTTKAGTH+SMSCLKEETCLPLGGYSVWSSLPPIN SS D+S
Sbjct: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258

Query: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 300
           KP+ILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG
Sbjct: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318

Query: 301 ESWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS 360
           ESWGYLGSRRFLLELDLQS++VSGL+N LID VFEIGSVGK SN G GNFFAHMTEVSSS
Sbjct: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDMVFEIGSVGKSSNHGSGNFFAHMTEVSSS 378

Query: 361 KNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK----------------- 420
           KNETWNALKLARESLP ENIKVSPASTTNPGIPPSSLMAFLAK                 
Sbjct: 379 KNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVVLDDFDTGFT 438

Query: 421 -----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIG 480
                SHLDDLHNINSSAIEAAALLVARTLYILA NK ELSSS L AIK+NTSLVEELIG
Sbjct: 439 NQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIG 498

Query: 481 CLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRT 540
           CLLNCDPGLSCELVKRYI+P++VCPNHYVGVILDEPSS PYP YVHDVSRF WNFLADRT
Sbjct: 499 CLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRT 558

Query: 541 SIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPN 600
           SI KENTSSVCSQNCDD+SEVCIGAETGKGTCV+STTRY+PAYSTRL FESG W+VLPPN
Sbjct: 559 SIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFESGYWSVLPPN 618

Query: 601 SSDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMK 642
           SSD +GAVDPVWTESNWNTIGLR+YT+QA AYDRFVLLGGITTTIL+YFAIVAVR SI+K
Sbjct: 619 SSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIK 678

BLAST of Cp4.1LG02g16620 vs. ExPASy TrEMBL
Match: A0A1S3C4S1 (Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1)

HSP 1 Score: 1075 bits (2779), Expect = 0.0
Identity = 551/665 (82.86%), Postives = 584/665 (87.82%), Query Frame = 0

Query: 1   MSFHVLHLLLFLTSVRLCLSDEHSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPG 60
           MS   L+LLLFLTS+RL  SDE  MESVPDLQNSMYL VDGYPCIRLLNLSGEIGCSNPG
Sbjct: 19  MSSQFLYLLLFLTSLRLSSSDEQKMESVPDLQNSMYLAVDGYPCIRLLNLSGEIGCSNPG 78

Query: 61  REKVVVPMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQK 120
           REKVV+PMINFKDADEIL+PSA+LVSMD ISSFF RLQDDS+FA+NVGGVLI+PGT IQ 
Sbjct: 79  REKVVLPMINFKDADEILEPSAVLVSMDAISSFFTRLQDDSHFANNVGGVLIEPGTGIQN 138

Query: 121 RTKGFSPAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKN 180
           RT+GFSPAQKFPQAKFAPY+K DYEWNPIGSG+MWN+YNFPVFLISESSIS +QEAASKN
Sbjct: 139 RTEGFSPAQKFPQAKFAPYKKNDYEWNPIGSGIMWNRYNFPVFLISESSISSIQEAASKN 198

Query: 181 VKDKKVYTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISS-DKS 240
           VK KK Y SNVAEFDLVMQTTKAGTH+SMSCLKEETCLPLGGYSVWSSLPPIN SS D+S
Sbjct: 199 VKSKKDYVSNVAEFDLVMQTTKAGTHSSMSCLKEETCLPLGGYSVWSSLPPINTSSSDQS 258

Query: 241 KPIILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 300
           KP+ILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG
Sbjct: 259 KPVILTVASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTG 318

Query: 301 ESWGYLGSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSS 360
           ESWGYLGSRRFLLELDLQS++VSGL+N LID                         VSSS
Sbjct: 319 ESWGYLGSRRFLLELDLQSDAVSGLSNRLIDM------------------------VSSS 378

Query: 361 KNETWNALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK----------------- 420
           KNETWNALKLARESLP ENIKVSPASTTNPGIPPSSLMAFLAK                 
Sbjct: 379 KNETWNALKLARESLPLENIKVSPASTTNPGIPPSSLMAFLAKNPQISGVVLDDFDTGFT 438

Query: 421 -----SHLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIG 480
                SHLDDLHNINSSAIEAAALLVARTLYILA NK ELSSS L AIK+NTSLVEELIG
Sbjct: 439 NQFYQSHLDDLHNINSSAIEAAALLVARTLYILAINKNELSSSVLTAIKVNTSLVEELIG 498

Query: 481 CLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRT 540
           CLLNCDPGLSCELVKRYI+P++VCPNHYVGVILDEPSS PYP YVHDVSRF WNFLADRT
Sbjct: 499 CLLNCDPGLSCELVKRYITPSSVCPNHYVGVILDEPSSAPYPDYVHDVSRFVWNFLADRT 558

Query: 541 SIRKENTSSVCSQNCDDKSEVCIGAETGKGTCVVSTTRYVPAYSTRLMFESGSWNVLPPN 600
           SI KENTSSVCSQNCDD+SEVCIGAETGKGTCV+STTRY+PAYSTRL FESG W+VLPPN
Sbjct: 559 SIPKENTSSVCSQNCDDRSEVCIGAETGKGTCVISTTRYIPAYSTRLKFESGYWSVLPPN 618

Query: 601 SSDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMK 642
           SSD +GAVDPVWTESNWNTIGLR+YT+QA AYDRFVLLGGITTTIL+YFAIVAVR SI+K
Sbjct: 619 SSDHLGAVDPVWTESNWNTIGLRIYTIQAAAYDRFVLLGGITTTILAYFAIVAVRSSIIK 659

BLAST of Cp4.1LG02g16620 vs. TAIR 10
Match: AT3G52640.1 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 802.7 bits (2072), Expect = 2.1e-232
Identity = 407/661 (61.57%), Postives = 502/661 (75.95%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGTHNS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL S++V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK----------------------S 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ K                      S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDLHNINSSAIEAAALLVARTLYILATNKKELSSSALNAIKLNTSLVEELIGCLLNCD 487
           HLDDL NINSS++ AAA +VARTLYILA++ K+ S+SAL +I +N S VEEL+ CLL C+
Sbjct: 436 HLDDLSNINSSSVVAAASVVARTLYILASDNKDTSNSALGSIHVNASFVEELLTCLLACE 495

Query: 488 PGLSCELVKRYISPTNVCPNHYVGVILDEPSSTPYPGYVHDVSRFAWNFLADRTSIRKEN 547
           PGLSC LVK YISPTN CP +Y GVIL EPSS PY GYV DVSRF WNFLAD+TS++K N
Sbjct: 496 PGLSCNLVKDYISPTNTCPGNYAGVILGEPSSKPYLGYVGDVSRFLWNFLADKTSVQKGN 555

Query: 548 TSSVCSQN-CDDKSEVCIGAETGK-GTCVVSTTRYVPAYSTRLMFESGSWNVLPPNSSDP 607
           T+SVCS+  C    EVCI AE+ K GTCVVSTTRYVPAYSTRL +  G+W +LP NSSD 
Sbjct: 556 TTSVCSKGVCSKTDEVCIKAESNKEGTCVVSTTRYVPAYSTRLKYNDGAWTILPQNSSDS 615

Query: 608 MGAVDPVWTESNWNTIGLRMYTVQATAYDRFVLLGGITTTILSYFAIVAVRGSIMKALKK 643
           MG VDPVWTESNW+T+ + +YTVQ +AYD  VL+ GIT T L+Y  I+A +  I KALK+
Sbjct: 616 MGMVDPVWTESNWDTLRVHVYTVQHSAYDNAVLVAGITVTTLAYIGILAAKSIITKALKQ 675

BLAST of Cp4.1LG02g16620 vs. TAIR 10
Match: AT3G52640.2 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 786.9 bits (2031), Expect = 1.2e-227
Identity = 407/690 (58.99%), Postives = 502/690 (72.75%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGTHNS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL S++V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK----------------------S 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ K                      S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDL-----------------------------HNINSSAIEAAALLVARTLYILATNK 487
           HLDDL                              NINSS++ AAA +VARTLYILA++ 
Sbjct: 436 HLDDLCKKSHSLSFSSFRSKPHFALLIPFWCCIAANINSSSVVAAASVVARTLYILASDN 495

Query: 488 KELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPS 547
           K+ S+SAL +I +N S VEEL+ CLL C+PGLSC LVK YISPTN CP +Y GVIL EPS
Sbjct: 496 KDTSNSALGSIHVNASFVEELLTCLLACEPGLSCNLVKDYISPTNTCPGNYAGVILGEPS 555

Query: 548 STPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQN-CDDKSEVCIGAETGK-GTCVVS 607
           S PY GYV DVSRF WNFLAD+TS++K NT+SVCS+  C    EVCI AE+ K GTCVVS
Sbjct: 556 SKPYLGYVGDVSRFLWNFLADKTSVQKGNTTSVCSKGVCSKTDEVCIKAESNKEGTCVVS 615

Query: 608 TTRYVPAYSTRLMFESGSWNVLPPNSSDPMGAVDPVWTESNWNTIGLRMYTVQATAYDRF 643
           TTRYVPAYSTRL +  G+W +LP NSSD MG VDPVWTESNW+T+ + +YTVQ +AYD  
Sbjct: 616 TTRYVPAYSTRLKYNDGAWTILPQNSSDSMGMVDPVWTESNWDTLRVHVYTVQHSAYDNA 675

BLAST of Cp4.1LG02g16620 vs. TAIR 10
Match: AT3G52640.3 (Zn-dependent exopeptidases superfamily protein )

HSP 1 Score: 675.2 bits (1741), Expect = 5.0e-194
Identity = 354/603 (58.71%), Postives = 436/603 (72.31%), Query Frame = 0

Query: 8   LLLFLTSVRLCLSDE-HSMESVPDLQNSMYLVVDGYPCIRLLNLSGEIGCSNPGREKVVV 67
           +LL +  + L L+DE  S+ESVPDLQ  MY+ VDG+PC+RLLNLSGEIGCSNPG  KVV 
Sbjct: 16  VLLSILPLHLSLADEITSIESVPDLQKLMYVAVDGFPCVRLLNLSGEIGCSNPGINKVVA 75

Query: 68  PMINFKDADEILQPSAILVSMDDISSFFARLQDDSNFASNVGGVLIKPGTEIQKRTKGFS 127
           P+I  KD  +++QP  ILV+ D++  FF R+  D +FAS +GGVL++ G+  Q++ KGFS
Sbjct: 76  PIIKLKDVKDLVQPHTILVTADEMEDFFTRVSTDLSFASKIGGVLVESGSNFQQKLKGFS 135

Query: 128 PAQKFPQAKFAPYQKIDYEWNPIGSGVMWNQYNFPVFLISESSISPVQEAASKNVKDKKV 187
           P ++FPQA+F+PY+ ++Y+WN   S +MW  YNFPV+L+SES IS V E  SK       
Sbjct: 136 PDKRFPQAQFSPYENVEYKWNSAASSIMWRNYNFPVYLLSESGISAVHEILSKKKMKHGT 195

Query: 188 YTSNVAEFDLVMQTTKAGTHNSMSCLKEETCLPLGGYSVWSSLPPINISSDKS-KPIILT 247
           YTS+VAEF++VM+TTKAGTHNS +CL+E TCLPLGGYSVWSSLPPI++SS  + KP++LT
Sbjct: 196 YTSDVAEFNMVMETTKAGTHNSEACLQEGTCLPLGGYSVWSSLPPISVSSSNNRKPVVLT 255

Query: 248 VASMDSASFFRDKSIGADSPISGLIALLAAVDALSHVDGLDDLHKQLVFVVFTGESWGYL 307
           VASMD+ASFFRDKS GADSPISGL+ALL AVDALS VDG+ +L KQLVF+V TGE+WGYL
Sbjct: 256 VASMDTASFFRDKSFGADSPISGLVALLGAVDALSRVDGISNLKKQLVFLVLTGETWGYL 315

Query: 308 GSRRFLLELDLQSNSVSGLNNTLIDTVFEIGSVGKKSNDGFGNFFAHMTEVSSSKNETWN 367
           GSRRFL ELDL S++V+GL+NT I+TV EIGSVGK  + G   FFAH T VSS  N T +
Sbjct: 316 GSRRFLHELDLHSDAVAGLSNTSIETVLEIGSVGKGLSGGINTFFAHKTRVSSVTNMTLD 375

Query: 368 ALKLARESLPFENIKVSPASTTNPGIPPSSLMAFLAK----------------------S 427
           ALK+A++SL  +NIK+  A T NPGIPPSSLMAF+ K                      S
Sbjct: 376 ALKIAQDSLASKNIKILSADTANPGIPPSSLMAFMRKNPQTSAVVLEDFDTNFVNKFYHS 435

Query: 428 HLDDL-----------------------------HNINSSAIEAAALLVARTLYILATNK 487
           HLDDL                              NINSS++ AAA +VARTLYILA++ 
Sbjct: 436 HLDDLCKKSHSLSFSSFRSKPHFALLIPFWCCIAANINSSSVVAAASVVARTLYILASDN 495

Query: 488 KELSSSALNAIKLNTSLVEELIGCLLNCDPGLSCELVKRYISPTNVCPNHYVGVILDEPS 547
           K+ S+SAL +I +N S VEEL+ CLL C+PGLSC LVK YISPTN CP +Y GVIL EPS
Sbjct: 496 KDTSNSALGSIHVNASFVEELLTCLLACEPGLSCNLVKDYISPTNTCPGNYAGVILGEPS 555

Query: 548 STPYPGYVHDVSRFAWNFLADRTSIRKENTSSVCSQN-CDDKSEVCIGAETGK-GTCVVS 556
           S PY GYV DVSRF WNFLAD+TS++K NT+SVCS+  C    EVCI AE+ K GTCVVS
Sbjct: 556 SKPYLGYVGDVSRFLWNFLADKTSVQKGNTTSVCSKGVCSKTDEVCIKAESNKEGTCVVS 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GUM52.9e-23161.57Nicastrin OS=Arabidopsis thaliana OX=3702 GN=At3g52640/At3g52650 PE=2 SV=1[more]
Q54JT71.2e-4325.63Nicastrin OS=Dictyostelium discoideum OX=44689 GN=ncstn PE=3 SV=2[more]
F0ZBA65.1e-4224.67Nicastrin OS=Dictyostelium purpureum OX=5786 GN=ncstn PE=1 SV=1[more]
Q925429.1e-2322.56Nicastrin OS=Homo sapiens OX=9606 GN=NCSTN PE=1 SV=2[more]
Q8CGU61.2e-2222.81Nicastrin OS=Rattus norvegicus OX=10116 GN=Ncstn PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023525268.10.096.69nicastrin [Cucurbita pepo subsp. pepo][more]
KAG7036691.10.095.78Nicastrin [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022948878.10.095.18nicastrin [Cucurbita moschata][more]
KAG6606989.10.095.33Nicastrin, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022997757.10.094.88nicastrin [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GAI90.095.18Nicastrin OS=Cucurbita moschata OX=3662 GN=LOC111452408 PE=3 SV=1[more]
A0A6J1K5Z20.094.88Nicastrin OS=Cucurbita maxima OX=3661 GN=LOC111492619 PE=3 SV=1[more]
A0A6J1CNM40.085.86Nicastrin OS=Momordica charantia OX=3673 GN=LOC111013258 PE=3 SV=1[more]
A0A1S3C4C70.086.02Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1[more]
A0A1S3C4S10.082.86Nicastrin OS=Cucumis melo OX=3656 GN=LOC103496866 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G52640.12.1e-23261.57Zn-dependent exopeptidases superfamily protein [more]
AT3G52640.21.2e-22758.99Zn-dependent exopeptidases superfamily protein [more]
AT3G52640.35.0e-19458.71Zn-dependent exopeptidases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF05450Nicastrincoord: 241..403
e-value: 6.0E-48
score: 163.3
NoneNo IPR availableGENE3D3.40.630.10Zn peptidasescoord: 182..446
e-value: 7.0E-15
score: 57.2
NoneNo IPR availableSUPERFAMILY53187Zn-dependent exopeptidasescoord: 215..433
IPR041084Nicastrin, small lobePFAMPF18266Ncstrn_smallcoord: 43..200
e-value: 2.8E-39
score: 134.8
IPR008710NicastrinPANTHERPTHR21092NICASTRINcoord: 17..641

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g16620.1Cp4.1LG02g16620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016485 protein processing
cellular_component GO:0005887 integral component of plasma membrane
cellular_component GO:0016021 integral component of membrane