Cp4.1LG02g15250 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g15250
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionphosphatidyl-N-methylethanolamine N-methyltransferase
LocationCp4.1LG02: 13541419 .. 13545050 (+)
RNA-Seq ExpressionCp4.1LG02g15250
SyntenyCp4.1LG02g15250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TAAAAAAATAGCTGAAATAATAATCCTATAAAAATTGGTCATTTAAAATTATGGTAGAAAATTTAGAATTGGGTCCACTAAAAAAGTCCAACCTTTTGGGCCCAAACTTTGGGCCCATATTATAGCCCAATAACAACGGCCCATTAGGGCCTATAGCCCATATTTGTTCCAGCGTCTATCGGTCATAAGGACTGTTCTGATTTCTGGATCTTCTTCTTCCTGAGAAAGTGGCGAATCCGGCGGTAAAGCTATCGAAATGGACGTTTTGGCTGTAATTGGAGTTTTGTTGCCATTTCCATACTATTACGTGTTATGGACACGGCCACAATTGTGGGTGAACTTGTGCGGAAAATGGTGCGAGCCTTCCAAATTGATGGCTCAGGTATCGGTTTTCCTCAAGCTGCTTCAGTTCCTCTCCATCCTCCATGTCTCCTCCTTCTCGTGGCCGCCGCCTCTCTACTTCTGGCCTCTCATGGCCTTCGGACAGTTCTTGAACTTCAGGTCTCTATCTCTCTCTCTTCCTTTCTCTCTGATGTTATTTGCGATCTGGAGGTAGAAGTGACAATTTATTAGGTGTTTTTGTTTACTAAGTAGTATGAAAGTGATATAAGACGTTAAATCTCGAGAGAATGTGGTTGTTAAGATTGAATTTAATGCCTATTGGTTTTGGAGATGATGATTAGCATTCTGAAATTGTGAATTTGGGATGAGTTTTGAGGAATTTTAAGTTTGGGATGTCTTTTGAGCAGTGTAGAAAGTAAAACCATTTGAAGTAAAAGTGTCTTTACAATTTTAAATGAAGATTCTAAAGGTATCTCGAATTCATTGTAATTTTGTTTCTTCATCAGGGAGAAGTGAAACTCTAATTCAGTACCTATTTTGAGTCTAGCGACTGTGGATCAGATCAATGACTTCAATACTCCAATTAACCATTCCTTGGATGTACATATGAAAAGGTTATCTACCTTTTTTGGATAAGAATTGGTTGTAATGCACCAAAAGAAATTATTAGAGAGTGAAGCACTTGATGGGAGAGAGACCAGCTTGACCTAGAGATTTGAATGATTACAGACAAAAGATTAAGACAATTACGAAATTCTTTGTCAAAAGATGATTACAAAATTATTTAGTATAGTAAAGCCATAAAGAAACATGAAGGTCAAGATTTTGCTCGCATTCTTTATTCTGGAAGGCCTTCCTCTTTCCCACACTTCCAATGAAATCAGAAAACATCTCAAACTACTGGTCACATCCTATTGATGCCGAAATTCCTTATTGGGCAATCTGTGGAAGGACAATAGATGCATCCTACAGCCTATTTATGTTGGATGCATTGTTCGGGGTATCCTCTTTCCACAATAGGATTGCTACATTGTGCTTCCAAGTACCAATGATCTCGTCCTTGCTATCCCTCTTTCAAGAATGCAACTTGGTTTTGTGTTTCCCTAAGAATGTTTTCCCTTTGGCCTTGAACAACTCTTGAGATCGTACAAAAAGTTAGTCCTTTCTGAAAATGCTATTTAGGGATTATCTGGGAAATTACCGACTTTTCAAGGATGTTGATATTGAAAGTGAAGAAACTTGGAATGCTTTTCACTTTATATTCGTTATATAAAGTTGCCTATTAAAATATTTTTGTAACTTTATGTGTGTGTGTGTGTTTGTATGTATACCTATATTCTTTATTAATTCTAATTGGAGCTAACTTTTAATCCTCCACTAGTTTCATTTAAGTTGATGAGTCAAGCACCTAGTGTATTTCTTGTGGTGTTAATGAAGACCTGTTTCCAATTAAAAAAAAAAAAAAAAGAAAGAACTGAGGCGGACGGGTTTACAATCTAGAATATCAAGATTTGCCCCTATCAATTACATAGTGCAATTGTAAAAAGTGAAAACCTGTGGAGCTTGTTGCATCGAAGTGAGCATCTCAGCGATTCAAACTCTGTAGCTGAAAAACTATTTCCATGCCTAGGATTCTGTGTACCTTAAAGACGAAATTGATTGCAACTTAAGAGGCTTTATCATAATGGTTAACAACGTCACACATTAATAGGTGGCTGGTAAGGATTAAGTACCTGAGGAATCGTTAAAGAATGTTCAATTTGATGGTGTCGAACTTTATTTCCTCTCAGATCCTTTTTTGTTGATTTCAGAAATAGAAAAGTGATGCTTGGAATAGTCAAACAAGCTTTATAGAGACCATTTTCCCAGTACAATTGTCTCGTTTGGTTGTTAGTCTTCACAAAATACGCCACCATTTACTATTATTGGGATATCATAAAGTACACATAACTAGGCTTACTTGAATGTTCATTTCTTATGCTCATGTTTGCAAATTCGAAAACAAGATGATAGGTAGAAATCAAACTAGATCCAAATGAAAAATTACAGTACCCGAGCACTTCAAGAAACTTGGACGGTGAGATCCCATATTGGTTGGAGAGGTGAAAGAAGCATTCCTTATAAGGGTGTGGAAATTTCTCCCTGACAGACACGTTTTAAAACCTTGAGGGGAAATCCTGAAGGAAAAGCCCAAAAAGGACAATATCTGCCGTTAGCTGTGGACTTGAGCTGTTACATGGACTCCCCATCCAAGAATAGACTAAGCATCAAAATCAGCTGCCTGAGTTTCCATTCAAAGGGAAGGAACTAAAGCACTAGTGCAAATCAAGATTGAAGGTTTAGGACATTCATAACTGTTATGTAACTTCACTATTCCAGGCACTGCTGGTGGTTTATTTTTACAATGTTGCTGCAGTTTTGCAGACTTGATTGGCTAAAATCTATATCGACAGCTGTGGCTTGTAAAGTTGAGTAATATTTTCTTTATCTTCTGCCATGCAACAGGGTCTACCAGTTACTCGGTGAAGCAGGTGTTTACTATGGAGTACGATTTGGGAAGAATATTCCGTGGGTAACAGAGTTTCCATTTGGATATATAAAAGATCCTCAGTACGTGGGAAGCATTTTGAGCCTTCTTGCTTGTCTTTCTTGGGTACCCTTGCAATATATTCTGCTCTGGACAATGGGATACGTCTTTATGATGCAAGTTGAATCCAAGGAAGATCTCACAACTCGAGCAAAGCCTCAAAATTAAATTTGTGAGAGACTAATCTTGGAACAGCCAAACATTGGATCAACACGTGGTCTGTGGAGGCTGCTTCAAGCATCCGTTAATAACATCCCCCAAATCAAACCTCTTGAAGTTTTGAATGAAATCCCGGGTTGAGTTATAGATGATATCCTCAACTTTATGATTTTGCCAATACTTTCTTAGAACTACTAGGAATAAGAATTTTTCCACCAACTTTGTGGATTCCTCTTCATTATGATGCCAGTTCTTCTTTTGGAAACTGGAAATCTAGAAAATTAGTCTACTTTCTTTGTGATTTACTTCAACAGCTTTGTTTTTGTAGTTTAAAAGCAAGCAGGAACTTAACTCTTGACAACGTTTCGTGCTACAAGAGGACTTTGTGGACCCTGTTCTTCGCTTTATAGAATCCGAAATGAAAATCTTCGTTTTGAAACATTTGATGTTCGATCACGAGACTTTTCTTTAAAAGTTTTTTATGTGATTCAAGACACAAGGGTGACAAGATTTAAGCGCAAGATATGGTTGGTTGAATGGTTAC

mRNA sequence

TAAAAAAATAGCTGAAATAATAATCCTATAAAAATTGGTCATTTAAAATTATGGTAGAAAATTTAGAATTGGGTCCACTAAAAAAGTCCAACCTTTTGGGCCCAAACTTTGGGCCCATATTATAGCCCAATAACAACGGCCCATTAGGGCCTATAGCCCATATTTGTTCCAGCGTCTATCGGTCATAAGGACTGTTCTGATTTCTGGATCTTCTTCTTCCTGAGAAAGTGGCGAATCCGGCGGTAAAGCTATCGAAATGGACGTTTTGGCTGTAATTGGAGTTTTGTTGCCATTTCCATACTATTACGTGTTATGGACACGGCCACAATTGTGGGTGAACTTGTGCGGAAAATGGTGCGAGCCTTCCAAATTGATGGCTCAGGTATCGGTTTTCCTCAAGCTGCTTCAGTTCCTCTCCATCCTCCATGTCTCCTCCTTCTCGTGGCCGCCGCCTCTCTACTTCTGGCCTCTCATGGCCTTCGGACAGTTCTTGAACTTCAGGGTCTACCAGTTACTCGGTGAAGCAGGTGTTTACTATGGAGTACGATTTGGGAAGAATATTCCGTGGGTAACAGAGTTTCCATTTGGATATATAAAAGATCCTCAGTACGTGGGAAGCATTTTGAGCCTTCTTGCTTGTCTTTCTTGGGTACCCTTGCAATATATTCTGCTCTGGACAATGGGATACGTCTTTATGATGCAAGTTGAATCCAAGGAAGATCTCACAACTCGAGCAAAGCCTCAAAATTAAATTTGTGAGAGACTAATCTTGGAACAGCCAAACATTGGATCAACACGTGGTCTGTGGAGGCTGCTTCAAGCATCCGTTAATAACATCCCCCAAATCAAACCTCTTGAAGTTTTGAATGAAATCCCGGGTTGAGTTATAGATGATATCCTCAACTTTATGATTTTGCCAATACTTTCTTAGAACTACTAGGAATAAGAATTTTTCCACCAACTTTGTGGATTCCTCTTCATTATGATGCCAGTTCTTCTTTTGGAAACTGGAAATCTAGAAAATTAGTCTACTTTCTTTGTGATTTACTTCAACAGCTTTGTTTTTGTAGTTTAAAAGCAAGCAGGAACTTAACTCTTGACAACGTTTCGTGCTACAAGAGGACTTTGTGGACCCTGTTCTTCGCTTTATAGAATCCGAAATGAAAATCTTCGTTTTGAAACATTTGATGTTCGATCACGAGACTTTTCTTTAAAAGTTTTTTATGTGATTCAAGACACAAGGGTGACAAGATTTAAGCGCAAGATATGGTTGGTTGAATGGTTAC

Coding sequence (CDS)

ATGGACGTTTTGGCTGTAATTGGAGTTTTGTTGCCATTTCCATACTATTACGTGTTATGGACACGGCCACAATTGTGGGTGAACTTGTGCGGAAAATGGTGCGAGCCTTCCAAATTGATGGCTCAGGTATCGGTTTTCCTCAAGCTGCTTCAGTTCCTCTCCATCCTCCATGTCTCCTCCTTCTCGTGGCCGCCGCCTCTCTACTTCTGGCCTCTCATGGCCTTCGGACAGTTCTTGAACTTCAGGGTCTACCAGTTACTCGGTGAAGCAGGTGTTTACTATGGAGTACGATTTGGGAAGAATATTCCGTGGGTAACAGAGTTTCCATTTGGATATATAAAAGATCCTCAGTACGTGGGAAGCATTTTGAGCCTTCTTGCTTGTCTTTCTTGGGTACCCTTGCAATATATTCTGCTCTGGACAATGGGATACGTCTTTATGATGCAAGTTGAATCCAAGGAAGATCTCACAACTCGAGCAAAGCCTCAAAATTAA

Protein sequence

MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSSFSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVGSILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN
Homology
BLAST of Cp4.1LG02g15250 vs. ExPASy Swiss-Prot
Match: Q9SAH5 (Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=PLMT PE=2 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 1.8e-68
Identity = 123/161 (76.40%), Postives = 137/161 (85.09%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           M +LA IGVLLPFP+Y+ LWT  Q WVNLCG+  +PS +MA+VS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
            SWPPPLYFWPLMAFGQFLNFRVYQLLGEAG YYGVRFGKNIPWVTEFPFG I+DPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAK 162
           SI+SLLACLSWVP QYILLW++GYVFMM +ESKED   RAK
Sbjct: 121 SIMSLLACLSWVPFQYILLWSLGYVFMMFLESKEDPNARAK 161

BLAST of Cp4.1LG02g15250 vs. NCBI nr
Match: XP_023523751.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 344 bits (883), Expect = 1.07e-119
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164

BLAST of Cp4.1LG02g15250 vs. NCBI nr
Match: KAG7036543.1 (Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 342 bits (878), Expect = 6.22e-119
Identity = 163/164 (99.39%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQ+
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. NCBI nr
Match: XP_022997915.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita maxima])

HSP 1 Score: 341 bits (875), Expect = 1.78e-118
Identity = 162/164 (98.78%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           MDVLAVIGVLLPFPYYY+LWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYLLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQ+
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. NCBI nr
Match: XP_022949314.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita moschata])

HSP 1 Score: 340 bits (872), Expect = 5.11e-118
Identity = 162/164 (98.78%), Postives = 163/164 (99.39%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQ LSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQILSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQ+
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. NCBI nr
Match: XP_004137657.1 (phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus] >KAE8650956.1 hypothetical protein Csa_002414 [Cucumis sativus])

HSP 1 Score: 313 bits (803), Expect = 1.71e-107
Identity = 148/164 (90.24%), Postives = 155/164 (94.51%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           M + AVIGVLLPFPYYYVLW RPQLWVNLCGK  EP KLMAQVSVFLKLLQFLSI+HVSS
Sbjct: 1   MGIFAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSL ACLSWVPL+YILLWT+GY+FMM VESKEDL TRAKPQ+
Sbjct: 121 SILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDLATRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. ExPASy TrEMBL
Match: A0A6J1KB80 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111492722 PE=4 SV=1)

HSP 1 Score: 341 bits (875), Expect = 8.63e-119
Identity = 162/164 (98.78%), Postives = 164/164 (100.00%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           MDVLAVIGVLLPFPYYY+LWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYLLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQ+
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. ExPASy TrEMBL
Match: A0A6J1GCG7 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111452701 PE=4 SV=1)

HSP 1 Score: 340 bits (872), Expect = 2.48e-118
Identity = 162/164 (98.78%), Postives = 163/164 (99.39%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQ LSILHVSS
Sbjct: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQILSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQ+
Sbjct: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. ExPASy TrEMBL
Match: A0A1S3CPQ5 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103502819 PE=4 SV=1)

HSP 1 Score: 312 bits (800), Expect = 2.37e-107
Identity = 148/164 (90.24%), Postives = 155/164 (94.51%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           M +LAVIGVLLPFPYYYVLW RPQLWVNLCGK  EP KLMAQVSVFLKLLQFLSI+HVSS
Sbjct: 1   MGILAVIGVLLPFPYYYVLWNRPQLWVNLCGKSREPCKLMAQVSVFLKLLQFLSIIHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYIKDPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILSL ACLSWVPL+YILLWT+GY+FMM VESKED  TRAKPQ+
Sbjct: 121 SILSLFACLSWVPLKYILLWTLGYIFMMHVESKEDPATRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. ExPASy TrEMBL
Match: A0A6J1DHC8 (phosphatidyl-N-methylethanolamine N-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111020909 PE=4 SV=1)

HSP 1 Score: 309 bits (792), Expect = 3.93e-106
Identity = 145/164 (88.41%), Postives = 155/164 (94.51%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           M ++A IGVLLPFP+YYVLWTRPQLW N CGKW EP KLMA+VS+FLKLLQFLSILHVSS
Sbjct: 1   MGIIAGIGVLLPFPFYYVLWTRPQLWANFCGKWREPCKLMARVSIFLKLLQFLSILHVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPLMAFGQFLNFRVYQLLGE+GVYYGVRFGKNIPWVTEFPFGYI+DPQYVG
Sbjct: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGESGVYYGVRFGKNIPWVTEFPFGYIRDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKPQN 164
           SILS+ ACLSWVPLQYILL T+GYVFMMQVESKED TTRAKPQ+
Sbjct: 121 SILSIFACLSWVPLQYILLLTLGYVFMMQVESKEDPTTRAKPQS 164

BLAST of Cp4.1LG02g15250 vs. ExPASy TrEMBL
Match: A0A5D2C6P4 (Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Gossypium darwinii OX=34276 GN=ES288_D06G147700v1 PE=3 SV=1)

HSP 1 Score: 278 bits (710), Expect = 1.25e-93
Identity = 127/162 (78.40%), Postives = 146/162 (90.12%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           M +LA IGVLLPFP+YY+LWT PQ WVNLCGK  +PSK+MA VS F+KL+QF+S+  VSS
Sbjct: 1   MGILAGIGVLLPFPFYYLLWTYPQSWVNLCGKGRDPSKVMALVSHFIKLIQFVSLFSVSS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
           FSWPPPLYFWPL AFGQFLNFRVYQLLGE+G YYGVRFGKNIPWV EFPFG+IKDPQYVG
Sbjct: 61  FSWPPPLYFWPLFAFGQFLNFRVYQLLGESGTYYGVRFGKNIPWVREFPFGFIKDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAKP 162
           SI+SL+ACLSWVP QYILLW++GY+FM+ +ES ED+TTRAKP
Sbjct: 121 SIMSLVACLSWVPFQYILLWSLGYLFMIHIESTEDVTTRAKP 162

BLAST of Cp4.1LG02g15250 vs. TAIR 10
Match: AT1G80860.2 (phospholipid N-methyltransferase )

HSP 1 Score: 260.0 bits (663), Expect = 1.3e-69
Identity = 123/161 (76.40%), Postives = 137/161 (85.09%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           M +LA IGVLLPFP+Y+ LWT  Q WVNLCG+  +PS +MA+VS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
            SWPPPLYFWPLMAFGQFLNFRVYQLLGEAG YYGVRFGKNIPWVTEFPFG I+DPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAK 162
           SI+SLLACLSWVP QYILLW++GYVFMM +ESKED   RAK
Sbjct: 121 SIMSLLACLSWVPFQYILLWSLGYVFMMFLESKEDPNARAK 161

BLAST of Cp4.1LG02g15250 vs. TAIR 10
Match: AT1G80860.1 (phospholipid N-methyltransferase )

HSP 1 Score: 260.0 bits (663), Expect = 1.3e-69
Identity = 123/161 (76.40%), Postives = 137/161 (85.09%), Query Frame = 0

Query: 1   MDVLAVIGVLLPFPYYYVLWTRPQLWVNLCGKWCEPSKLMAQVSVFLKLLQFLSILHVSS 60
           M +LA IGVLLPFP+Y+ LWT  Q WVNLCG+  +PS +MA+VS  LK  Q LS+  V+S
Sbjct: 1   MGLLAAIGVLLPFPFYWWLWTNAQSWVNLCGRERDPSTVMARVSHVLKAAQLLSLFSVAS 60

Query: 61  FSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGVYYGVRFGKNIPWVTEFPFGYIKDPQYVG 120
            SWPPPLYFWPLMAFGQFLNFRVYQLLGEAG YYGVRFGKNIPWVTEFPFG I+DPQYVG
Sbjct: 61  LSWPPPLYFWPLMAFGQFLNFRVYQLLGEAGTYYGVRFGKNIPWVTEFPFGVIRDPQYVG 120

Query: 121 SILSLLACLSWVPLQYILLWTMGYVFMMQVESKEDLTTRAK 162
           SI+SLLACLSWVP QYILLW++GYVFMM +ESKED   RAK
Sbjct: 121 SIMSLLACLSWVPFQYILLWSLGYVFMMFLESKEDPNARAK 161

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SAH51.8e-6876.40Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
XP_023523751.11.07e-119100.00phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita pepo subsp. pep... [more]
KAG7036543.16.22e-11999.39Phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita argyrosperma su... [more]
XP_022997915.11.78e-11898.78phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita maxima][more]
XP_022949314.15.11e-11898.78phosphatidyl-N-methylethanolamine N-methyltransferase [Cucurbita moschata][more]
XP_004137657.11.71e-10790.24phosphatidyl-N-methylethanolamine N-methyltransferase [Cucumis sativus] >KAE8650... [more]
Match NameE-valueIdentityDescription
A0A6J1KB808.63e-11998.78phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita maxima OX=366... [more]
A0A6J1GCG72.48e-11898.78phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucurbita moschata OX=3... [more]
A0A1S3CPQ52.37e-10790.24phosphatidyl-N-methylethanolamine N-methyltransferase OS=Cucumis melo OX=3656 GN... [more]
A0A6J1DHC83.93e-10688.41phosphatidyl-N-methylethanolamine N-methyltransferase OS=Momordica charantia OX=... [more]
A0A5D2C6P41.25e-9378.40Phosphatidyl-N-methylethanolamine N-methyltransferase OS=Gossypium darwinii OX=3... [more]
Match NameE-valueIdentityDescription
AT1G80860.21.3e-6976.40phospholipid N-methyltransferase [more]
AT1G80860.11.3e-6976.40phospholipid N-methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.20.120.1630coord: 32..160
e-value: 1.2E-5
score: 27.2
NoneNo IPR availablePANTHERPTHR15458:SF5PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASEcoord: 8..162
IPR007318Phospholipid methyltransferasePFAMPF04191PEMTcoord: 71..151
e-value: 1.9E-10
score: 41.1
IPR024960Phosphatidyl-N-methylethanolamine/Phosphatidylethanolamine N-methyltransferasePANTHERPTHR15458PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASEcoord: 8..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g15250.1Cp4.1LG02g15250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006656 phosphatidylcholine biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004608 phosphatidylethanolamine N-methyltransferase activity
molecular_function GO:0080101 phosphatidyl-N-dimethylethanolamine N-methyltransferase activity
molecular_function GO:0000773 phosphatidyl-N-methylethanolamine N-methyltransferase activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity