Cp4.1LG02g14730 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g14730
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPhospholipase D
LocationCp4.1LG02: 13756953 .. 13761277 (-)
RNA-Seq ExpressionCp4.1LG02g14730
SyntenyCp4.1LG02g14730
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCCTTTTTCACATGCTTGCAATCATTCCATTTATGCATAAAACTCCTTCCATTTTCGTTTCCCCTCTGCTTCTTCTTCTTCTTCTTCTTCTTCGCTGAAGCTGAAGAAAAAATCTGCAGAAACAGTACGGAAAGCTTCGAGAAAGGTATTTCCATGCCTCTGCTGCTCTTCAACTTTGTTTCTATTTACTTCTTCTTCTTCTTGCTCATGTTTCTTACTAAAGCGTGTGTTATGAGTTCGTTCGACTATTTTACTTTTCAATCGCTTTCTGTTCTGTTAAATCTGTGTTGCTGTGATTAGTTCTCCGATTTTCTTTAGTTTTCTCTTCAAATCAATAACTGAATAGGATCGGTTTAGAGGATCTTGCTACTCGACGGAGCTCTGCTATCTGATTAGCAGAATGCGACAGATCTCTGCCTCCTACGGTGGTTAGTCGCCGTCAGATCCGGTGACTCTGAGTGCCGTTTGGGCCGTAGGAGGGGACGGATCTCCGACCTTCGGTAGTCGGAGAGTTGGACGGCCGTCGACGGCTGGAGTACCGTCGAGAGAGAGTGAGTCCGTACGTAGGAAATTTTTATATTACTATTTCTTATGTGCGTGTGGTTGTGGCACCACTTGCTGTTTATCGCGTTCGCTCGTGGGCTCCGCCAGGTCTATTCATCCAAAACCCACAAAGAACGTTTTACCTTTCTTTTTTGGAAAATAATTAGGATCTAAAAAACCTTTAAAATATCCTTTATAACATTAAAGTTTCAATAATAACGCACCAACTTTCATTAGTTTTAAAAAAAACTTTCTAATGATATGATATTGTCCACTTTAAGCATAAATTCTAGTGATTTTATTTTTGGCTTTCTAAGAAGCCTCAAACCAATGTAGAGGTATTCCTTGATTATAAACCTATAATTATTTCCTAAACGAGCTGATATGGGACTTTCATCATTCAACCGTCCGCAAATACTTTCATAAAAATTAATGAGAATAAACAAATTATTAACATTAGTGGTAACAATTTAACCTAATAAACTTTAAATGCTTAAAATTCATAACTAGAATTTTGGAGCATCAAATTGTGGGATGAAAAATAAATTATTGATGAACCCACCTACTATATTATCATGAATGAAAATTATAGTGTATAACCTCCCTATTAATTTTCATTAAAATACTTTTGGTGTTGATTTTGAGGTTAATAAATTTGATTATAGGGCCTAATTCAAGAGGATTACGTTAATTTTGTGTGGCAGTTGAGAAATGGCGCACATTCTGCTTCATGGCACCCTTCATGTCACCATTTATGAGGTGGATAAGCTTCACTCCGGTGCTCGCAATTTCTTGAAATCGGTAACTCTCTACTTTATCTTAATTAACTTTTTTTGAGGTGGATAAGCTTCATTCCGGTGGAAGCTAAAAGTTGATAACTTTTTTTGGGTGTGCAGTTGGTTGAAAACGTAGAGGAAGCTGTGGGGTTTGGCAGAGGAGTAACGAGGCTGTACGCCACCATTGATTTAGAGAAGGCAAGAGTTGGAAGAACAAGAAGACTCGAGAAAGAACATTCAAATCCAAAATGGTACGAAACTTTCCACATCTACTGTGCTCACATGGCTTCCAACATCATCTTCACTGTCAAAGACGACAATCCCATTGGAGCAACCTTAATCGGCAGAGCATATTTGCCTGTCAAAGAGATCATAAGCGGTGAAGAAGTTGACCAATGGGTTCCTATCTTAGACGAACGCAAGAAGCCCATTCGTGGAGACCCCAAAATTCATGTCAAATTGCAATACTTCGATGTCACTAAGGATCAAAACTGGGGTCGTGGCATCAAAAGCTCCAAGTTTCCTGGAGTTCCCTACACTTATTTCTCTCAACGACAAGGTTGTAAGGTAACTTTGTACCAAGATGCTCATGTTCCTGACAACTTTATCCCCAAAATTCCTCTTGCTGGTGGCAAGTTTTATGAGCCAAATAGATGTTGGGAAGATATTTTTGATGCAATTATTAATGCTAAACACATGATTTACATTACTGGTTGGTCTGTTTACACTGAGATTTCTTTGGTGAGAGATAAAAGAAGGCCAAAGCCTGGTGGAGATATGATGCTTGGTGAATTGCTTAAGAAAAAAGCAAATGAAGGAGTTAGAGTTCTTTTGCTTGTTTGGAATGATACTACTTCTGTGCCTATCCTGAAGGAGGACGGGCTAATGGCTACTCATGATGAAGATACTGCTAAGTTCTTTGCTAATACTGATGTGCATTGTGTTCTGTGTCCTCGCAATCCTGATGATGGGGCTAATGTTATTCAGGATATTGCCGTTGGGACAATGTTTACTCATCATCAAAAGATTGTTGTTGTGGACAGTGCTTTGCCTAATGGAGATCCAAATCGAAGGAGGATTGTGAGTTTTGTTGGTGGGATTGATCTTTGTGATGGTAGATATGATACTCCATTTCATTCCCTTTTCAGGACTTTGGATTCAGCTCATCATGATGATTTCCATCAGCCAAACTTCACCGGTACTTCCATAAACAAAGGCGGTCCGAGAGAGCCATGGCACGATATCCATTCCCGCCTTGAAGGACCGGTTGCGTGGGATGTTTTGTACAACTTTGAGCAGAGGTGGAGGAAGCAAGGTGGAAAGGATTTGCTTGTGAAATTAAGGGAGTTGGATGCCATCTTCATCCCTCCATCACCTGTCATGTTCCCTGATGACTTTGATAGTTGGAATGCTCAAGTTTTCAGGTCCATCGATGGCGGGGCGGCTTTTGGCTTCCCCGACTCGCCCGAAGAAGCAGCCAAAGCTGGACTTATAAGTGGAAAGGACAACATTATTGATAGAAGCATCCAAGATGCTTACATTAATGCCATTAGAAGAGCAAAGAATTTCATATACATTGAAAATCAGTATTTCCTTGGAAGCTCGTTTGAATGGAGAGGTACTGATGTGAAGCCGGAGATGATCAATGCCCTGCATCTAATCCCAAAGGAACTTTCACTTAAGATTGTGAGTAAGATTGAAGCTCGAGAGCGATTCACTGTGTACGTCGTCGTCCCGATGTGGCCGGAAGGATTCCCAGAGGGGGGAACAGTTCAAGCTATATTAGATTGGCAAAGAAGAACAATGGAGATGATGTATAAAGATATAGCTCTAGCCCTTAGAGTTAATGGCATCGAAGAGGATCCTAGAAACTATCTGACTTTCTTTTGTGTAGGCAATCGCGAAGTTAAGAAGCCTGGAGAATATGAGCCATCAGAATCCCCTGATGAAAATTCCAGTTACCTTAGAGCCCAACAAGCCAGGCGCTTCATGATTTATGTTCATGCCAAGATGATGATCGGTAAGTTTTGACTTTCTCTTAGTTCTAGAAATTCTCTTTCCGTAGATATTATCCTTTTTCGCCTTTTCCTTCCAGAGCTTTCCCTCACCCTTATAAAGAATGTTTCGTTCCTCTCTCCTACCGATGTGCGATCATGAGCTATTCCCAACAGATGACGAATTCTTGCCGAAAAATGATGACCTATTCTTGCCCGAAAATGAAACTTGTCATTTAACCTTAAACCTTAGTAAAATTAAAACTAGCCTTATCTCAGAGGTGGTAGTAAATTTTAGTTGAGAAAGCTAATGTTATTTTGTTTAAAACAGTTGATGATGAATACATAATAGTGGGATCAGCCAACATCAACCAAAGATCAATGGATGGTGCAAGGGACTCAGAGATAGCCATGGGAGCTTACCAGCCATTTCATCTGTCATCAAGAGAGCCAGCAAGAGGCCAAGTCCATGGCTTCCGTATGGCATTATGGTTTGAACATTTAGGATTACTCCATGACTCCTTCCTCTTTCCAGAAAGCATTGAGTGTGTAAGAAAAGTTAACCAACTTGCTGATAAATATTGGGACCTATACTCAAGTGAAACCTTTGAACACGACCTTCCCGGCCACCTGCTTCGCTACCCGATCAGCATCTCCGGTGACGGTGAAATTACCGAGCTTCCTGGGTTCGAGTTCTTCCCGGATACTAAGGCGCGAGTCCTAGGTAACAAGTCTAATTATCTCCCTCCGATCCTCACTACTTAAAAGTTACCACTTTTCCTAACTGTTGTAGAAAAGAAAATGCTGGCTTTCCTGTTCTTTCTCTTCAAGTTCTGCCTTTCCTACTCTGTTGTGATGGGAACGCAAGAAATCCTCGTGTTTGTGAAATGGTTTAATGTTTATGAATGTGATTGAAGTTGCTTTCTAATAAAAACAGGGACTTTGTTTGTAAGGGATTAAGGGTTGATTTATGTTATTTAATTTCTTAAAAAAATTATTTCAATCGGATT

mRNA sequence

TATCCTTTTTCACATGCTTGCAATCATTCCATTTATGCATAAAACTCCTTCCATTTTCGTTTCCCCTCTGCTTCTTCTTCTTCTTCTTCTTCTTCGCTGAAGCTGAAGAAAAAATCTGCAGAAACAGTACGGAAAGCTTCGAGAAAGTTGAGAAATGGCGCACATTCTGCTTCATGGCACCCTTCATGTCACCATTTATGAGGTGGATAAGCTTCACTCCGGTGCTCGCAATTTCTTGAAATCGTTGGTTGAAAACGTAGAGGAAGCTGTGGGGTTTGGCAGAGGAGTAACGAGGCTGTACGCCACCATTGATTTAGAGAAGGCAAGAGTTGGAAGAACAAGAAGACTCGAGAAAGAACATTCAAATCCAAAATGGTACGAAACTTTCCACATCTACTGTGCTCACATGGCTTCCAACATCATCTTCACTGTCAAAGACGACAATCCCATTGGAGCAACCTTAATCGGCAGAGCATATTTGCCTGTCAAAGAGATCATAAGCGGTGAAGAAGTTGACCAATGGGTTCCTATCTTAGACGAACGCAAGAAGCCCATTCGTGGAGACCCCAAAATTCATGTCAAATTGCAATACTTCGATGTCACTAAGGATCAAAACTGGGGTCGTGGCATCAAAAGCTCCAAGTTTCCTGGAGTTCCCTACACTTATTTCTCTCAACGACAAGGTTGTAAGGTAACTTTGTACCAAGATGCTCATGTTCCTGACAACTTTATCCCCAAAATTCCTCTTGCTGGTGGCAAGTTTTATGAGCCAAATAGATGTTGGGAAGATATTTTTGATGCAATTATTAATGCTAAACACATGATTTACATTACTGGTTGGTCTGTTTACACTGAGATTTCTTTGGTGAGAGATAAAAGAAGGCCAAAGCCTGGTGGAGATATGATGCTTGGTGAATTGCTTAAGAAAAAAGCAAATGAAGGAGTTAGAGTTCTTTTGCTTGTTTGGAATGATACTACTTCTGTGCCTATCCTGAAGGAGGACGGGCTAATGGCTACTCATGATGAAGATACTGCTAAGTTCTTTGCTAATACTGATGTGCATTGTGTTCTGTGTCCTCGCAATCCTGATGATGGGGCTAATGTTATTCAGGATATTGCCGTTGGGACAATGTTTACTCATCATCAAAAGATTGTTGTTGTGGACAGTGCTTTGCCTAATGGAGATCCAAATCGAAGGAGGATTGTGAGTTTTGTTGGTGGGATTGATCTTTGTGATGGTAGATATGATACTCCATTTCATTCCCTTTTCAGGACTTTGGATTCAGCTCATCATGATGATTTCCATCAGCCAAACTTCACCGGTACTTCCATAAACAAAGGCGGTCCGAGAGAGCCATGGCACGATATCCATTCCCGCCTTGAAGGACCGGTTGCGTGGGATGTTTTGTACAACTTTGAGCAGAGGTGGAGGAAGCAAGGTGGAAAGGATTTGCTTGTGAAATTAAGGGAGTTGGATGCCATCTTCATCCCTCCATCACCTGTCATGTTCCCTGATGACTTTGATAGTTGGAATGCTCAAGTTTTCAGGTCCATCGATGGCGGGGCGGCTTTTGGCTTCCCCGACTCGCCCGAAGAAGCAGCCAAAGCTGGACTTATAAGTGGAAAGGACAACATTATTGATAGAAGCATCCAAGATGCTTACATTAATGCCATTAGAAGAGCAAAGAATTTCATATACATTGAAAATCAGTATTTCCTTGGAAGCTCGTTTGAATGGAGAGGTACTGATGTGAAGCCGGAGATGATCAATGCCCTGCATCTAATCCCAAAGGAACTTTCACTTAAGATTGTGAGTAAGATTGAAGCTCGAGAGCGATTCACTGTGTACGTCGTCGTCCCGATGTGGCCGGAAGGATTCCCAGAGGGGGGAACAGTTCAAGCTATATTAGATTGGCAAAGAAGAACAATGGAGATGATGTATAAAGATATAGCTCTAGCCCTTAGAGTTAATGGCATCGAAGAGGATCCTAGAAACTATCTGACTTTCTTTTGTGTAGGCAATCGCGAAGTTAAGAAGCCTGGAGAATATGAGCCATCAGAATCCCCTGATGAAAATTCCAGTTACCTTAGAGCCCAACAAGCCAGGCGCTTCATGATTTATGTTCATGCCAAGATGATGATCGTTGATGATGAATACATAATAGTGGGATCAGCCAACATCAACCAAAGATCAATGGATGGTGCAAGGGACTCAGAGATAGCCATGGGAGCTTACCAGCCATTTCATCTGTCATCAAGAGAGCCAGCAAGAGGCCAAGTCCATGGCTTCCGTATGGCATTATGGTTTGAACATTTAGGATTACTCCATGACTCCTTCCTCTTTCCAGAAAGCATTGAGTGTGTAAGAAAAGTTAACCAACTTGCTGATAAATATTGGGACCTATACTCAAGTGAAACCTTTGAACACGACCTTCCCGGCCACCTGCTTCGCTACCCGATCAGCATCTCCGGTGACGGTGAAATTACCGAGCTTCCTGGGTTCGAGTTCTTCCCGGATACTAAGGCGCGAGTCCTAGGTAACAAGTCTAATTATCTCCCTCCGATCCTCACTACTTAAAAGTTACCACTTTTCCTAACTGTTGTAGAAAAGAAAATGCTGGCTTTCCTGTTCTTTCTCTTCAAGTTCTGCCTTTCCTACTCTGTTGTGATGGGAACGCAAGAAATCCTCGTGTTTGTGAAATGGTTTAATGTTTATGAATGTGATTGAAGTTGCTTTCTAATAAAAACAGGGACTTTGTTTGTAAGGGATTAAGGGTTGATTTATGTTATTTAATTTCTTAAAAAAATTATTTCAATCGGATT

Coding sequence (CDS)

ATGGCGCACATTCTGCTTCATGGCACCCTTCATGTCACCATTTATGAGGTGGATAAGCTTCACTCCGGTGCTCGCAATTTCTTGAAATCGTTGGTTGAAAACGTAGAGGAAGCTGTGGGGTTTGGCAGAGGAGTAACGAGGCTGTACGCCACCATTGATTTAGAGAAGGCAAGAGTTGGAAGAACAAGAAGACTCGAGAAAGAACATTCAAATCCAAAATGGTACGAAACTTTCCACATCTACTGTGCTCACATGGCTTCCAACATCATCTTCACTGTCAAAGACGACAATCCCATTGGAGCAACCTTAATCGGCAGAGCATATTTGCCTGTCAAAGAGATCATAAGCGGTGAAGAAGTTGACCAATGGGTTCCTATCTTAGACGAACGCAAGAAGCCCATTCGTGGAGACCCCAAAATTCATGTCAAATTGCAATACTTCGATGTCACTAAGGATCAAAACTGGGGTCGTGGCATCAAAAGCTCCAAGTTTCCTGGAGTTCCCTACACTTATTTCTCTCAACGACAAGGTTGTAAGGTAACTTTGTACCAAGATGCTCATGTTCCTGACAACTTTATCCCCAAAATTCCTCTTGCTGGTGGCAAGTTTTATGAGCCAAATAGATGTTGGGAAGATATTTTTGATGCAATTATTAATGCTAAACACATGATTTACATTACTGGTTGGTCTGTTTACACTGAGATTTCTTTGGTGAGAGATAAAAGAAGGCCAAAGCCTGGTGGAGATATGATGCTTGGTGAATTGCTTAAGAAAAAAGCAAATGAAGGAGTTAGAGTTCTTTTGCTTGTTTGGAATGATACTACTTCTGTGCCTATCCTGAAGGAGGACGGGCTAATGGCTACTCATGATGAAGATACTGCTAAGTTCTTTGCTAATACTGATGTGCATTGTGTTCTGTGTCCTCGCAATCCTGATGATGGGGCTAATGTTATTCAGGATATTGCCGTTGGGACAATGTTTACTCATCATCAAAAGATTGTTGTTGTGGACAGTGCTTTGCCTAATGGAGATCCAAATCGAAGGAGGATTGTGAGTTTTGTTGGTGGGATTGATCTTTGTGATGGTAGATATGATACTCCATTTCATTCCCTTTTCAGGACTTTGGATTCAGCTCATCATGATGATTTCCATCAGCCAAACTTCACCGGTACTTCCATAAACAAAGGCGGTCCGAGAGAGCCATGGCACGATATCCATTCCCGCCTTGAAGGACCGGTTGCGTGGGATGTTTTGTACAACTTTGAGCAGAGGTGGAGGAAGCAAGGTGGAAAGGATTTGCTTGTGAAATTAAGGGAGTTGGATGCCATCTTCATCCCTCCATCACCTGTCATGTTCCCTGATGACTTTGATAGTTGGAATGCTCAAGTTTTCAGGTCCATCGATGGCGGGGCGGCTTTTGGCTTCCCCGACTCGCCCGAAGAAGCAGCCAAAGCTGGACTTATAAGTGGAAAGGACAACATTATTGATAGAAGCATCCAAGATGCTTACATTAATGCCATTAGAAGAGCAAAGAATTTCATATACATTGAAAATCAGTATTTCCTTGGAAGCTCGTTTGAATGGAGAGGTACTGATGTGAAGCCGGAGATGATCAATGCCCTGCATCTAATCCCAAAGGAACTTTCACTTAAGATTGTGAGTAAGATTGAAGCTCGAGAGCGATTCACTGTGTACGTCGTCGTCCCGATGTGGCCGGAAGGATTCCCAGAGGGGGGAACAGTTCAAGCTATATTAGATTGGCAAAGAAGAACAATGGAGATGATGTATAAAGATATAGCTCTAGCCCTTAGAGTTAATGGCATCGAAGAGGATCCTAGAAACTATCTGACTTTCTTTTGTGTAGGCAATCGCGAAGTTAAGAAGCCTGGAGAATATGAGCCATCAGAATCCCCTGATGAAAATTCCAGTTACCTTAGAGCCCAACAAGCCAGGCGCTTCATGATTTATGTTCATGCCAAGATGATGATCGTTGATGATGAATACATAATAGTGGGATCAGCCAACATCAACCAAAGATCAATGGATGGTGCAAGGGACTCAGAGATAGCCATGGGAGCTTACCAGCCATTTCATCTGTCATCAAGAGAGCCAGCAAGAGGCCAAGTCCATGGCTTCCGTATGGCATTATGGTTTGAACATTTAGGATTACTCCATGACTCCTTCCTCTTTCCAGAAAGCATTGAGTGTGTAAGAAAAGTTAACCAACTTGCTGATAAATATTGGGACCTATACTCAAGTGAAACCTTTGAACACGACCTTCCCGGCCACCTGCTTCGCTACCCGATCAGCATCTCCGGTGACGGTGAAATTACCGAGCTTCCTGGGTTCGAGTTCTTCCCGGATACTAAGGCGCGAGTCCTAGGTAACAAGTCTAATTATCTCCCTCCGATCCTCACTACTTAA

Protein sequence

MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKVTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYNFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITELPGFEFFPDTKARVLGNKSNYLPPILTT
Homology
BLAST of Cp4.1LG02g14730 vs. ExPASy Swiss-Prot
Match: Q41142 (Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1)

HSP 1 Score: 1422.1 bits (3680), Expect = 0.0e+00
Identity = 646/808 (79.95%), Postives = 736/808 (91.09%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHS-GARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARV 60
           MA I LHGTLHVTIYEVDKLHS G  +F + LVEN+EE VGFG+GV++LYATIDLEKARV
Sbjct: 1   MAQISLHGTLHVTIYEVDKLHSGGGPHFFRKLVENIEETVGFGKGVSKLYATIDLEKARV 60

Query: 61  GRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEE 120
           GRTR LE E SNP+WYE+FH+YCAH ASN+IFTVKDDNPIGATLIGRAY+PV+E++ GEE
Sbjct: 61  GRTRILENEQSNPRWYESFHVYCAHQASNVIFTVKDDNPIGATLIGRAYVPVEELLDGEE 120

Query: 121 VDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCK 180
           +D+WV ILDE K P+    KIHVKLQYF+VTKD+NWG+GI+SSK+PGVPYTYFSQRQGCK
Sbjct: 121 IDRWVEILDEDKNPVHSGSKIHVKLQYFEVTKDRNWGQGIRSSKYPGVPYTYFSQRQGCK 180

Query: 181 VTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVR 240
           V+LYQDAH+PD F+P+IPLAGG +YEP+RCWED+FDAI NAKH+IYITGWSVYTEISL+R
Sbjct: 181 VSLYQDAHIPDKFVPQIPLAGGNYYEPHRCWEDVFDAITNAKHLIYITGWSVYTEISLIR 240

Query: 241 DKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFAN 300
           D RRPKPGGD+ LGELLKKKA+EGVRVL+LVW+D TSV +LK+DGLMATHDE+T  FF N
Sbjct: 241 DSRRPKPGGDITLGELLKKKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETEHFFQN 300

Query: 301 TDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDL 360
           TDVHCVLCPRNPDDG + +QD+ + TMFTHHQKIVVVDSA+PNGD  RRRIVSFVGG+DL
Sbjct: 301 TDVHCVLCPRNPDDGGSFVQDLQISTMFTHHQKIVVVDSAMPNGDSQRRRIVSFVGGLDL 360

Query: 361 CDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLY 420
           CDGRYD+PFHSLFRTLDSAHHDDFHQPNF G SI KGGPREPWHDIHSRLEGP+AWDVL+
Sbjct: 361 CDGRYDSPFHSLFRTLDSAHHDDFHQPNFAGASIEKGGPREPWHDIHSRLEGPIAWDVLF 420

Query: 421 NFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSP 480
           NFEQRWRKQGGKDLL++LREL+ + IPPSPVM+PDDF++WN Q+FRSIDGGAAFGFP++P
Sbjct: 421 NFEQRWRKQGGKDLLIQLRELEDVIIPPSPVMYPDDFEAWNVQLFRSIDGGAAFGFPETP 480

Query: 481 EEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMIN 540
           E+A +AGL+SGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSF W    +KPE IN
Sbjct: 481 EDAPEAGLVSGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFGWSPDGIKPEDIN 540

Query: 541 ALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDI 600
           ALHLIPKELSLKI+SKI A ERFTVY+VVPMWPEG PE  +VQAILDWQ+RTMEMMYKDI
Sbjct: 541 ALHLIPKELSLKILSKIAAGERFTVYIVVPMWPEGIPESASVQAILDWQKRTMEMMYKDI 600

Query: 601 ALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHA 660
             AL+ NGI EDPRNYLTFFC+GNREVKK GEYEP+E P+ ++ Y+RAQ+ARRFMIYVH 
Sbjct: 601 VQALKANGIIEDPRNYLTFFCLGNREVKKSGEYEPAEKPEPDTDYIRAQEARRFMIYVHT 660

Query: 661 KMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFE 720
           KMMIVDDEYII+GSANINQRSMDGARDSEIAMGAYQP HLS+R+PARGQ+HGFRM+LW+E
Sbjct: 661 KMMIVDDEYIIIGSANINQRSMDGARDSEIAMGAYQPHHLSTRQPARGQIHGFRMSLWYE 720

Query: 721 HLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITE 780
           HLG+L +SFL PES ECVRKVNQ+A+KYWDLYSSET EHDLPGHLLRYPI ++ +G++TE
Sbjct: 721 HLGMLDESFLNPESEECVRKVNQMAEKYWDLYSSETLEHDLPGHLLRYPIGVASEGDVTE 780

Query: 781 LPGFEFFPDTKARVLGNKSNYLPPILTT 808
           LPG EFFPDTKARVLG KS+YLPPILTT
Sbjct: 781 LPGTEFFPDTKARVLGAKSDYLPPILTT 808

BLAST of Cp4.1LG02g14730 vs. ExPASy Swiss-Prot
Match: P86387 (Phospholipase D alpha 1 OS=Carica papaya OX=3649 GN=PLD1 PE=1 SV=1)

HSP 1 Score: 1387.5 bits (3590), Expect = 0.0e+00
Identity = 633/808 (78.34%), Postives = 724/808 (89.60%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGA-RNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARV 60
           MAH L+HGTLH T+YEVDKLHSG    F   ++ NVE  +G G+GVT+LYATIDLE+ARV
Sbjct: 1   MAHYLMHGTLHATVYEVDKLHSGGISGFFGKILANVEGTIGIGKGVTQLYATIDLERARV 60

Query: 61  GRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEE 120
           GRTR ++ E +NPKWYE+FHIYCAHMASN++FTVKDDNPIGATLIGRAY+PV+E+I G++
Sbjct: 61  GRTRIIKDEPNNPKWYESFHIYCAHMASNVVFTVKDDNPIGATLIGRAYVPVEELIRGDQ 120

Query: 121 VDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCK 180
           VD+WV ILDE K PI GD KIHVKLQ+FDV KD NW  GIK +++ GVPYT++SQR+GC+
Sbjct: 121 VDRWVEILDEDKNPIEGDSKIHVKLQFFDVKKDSNWNMGIKGARYLGVPYTFYSQRRGCR 180

Query: 181 VTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVR 240
           V+LYQDAHVPD FIPKIPLAGGK+YEP+RCWED+FDAI NA+H+IYITGWSVYTEI+L+R
Sbjct: 181 VSLYQDAHVPDGFIPKIPLAGGKYYEPHRCWEDVFDAITNARHLIYITGWSVYTEITLIR 240

Query: 241 DKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFAN 300
           D RRPKPGGD+ LGELLK+KA+EGV+VL+LVW+D TSV +LK+DGLMATHDE+TA +F N
Sbjct: 241 DSRRPKPGGDVTLGELLKQKASEGVKVLMLVWDDRTSVGLLKKDGLMATHDEETANYFQN 300

Query: 301 TDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDL 360
           TDVHCVLCPRNPDDG + +Q + + TMFTHHQKIVVVD  +P+G+   RRIVSFVGGIDL
Sbjct: 301 TDVHCVLCPRNPDDGGSFVQGLQISTMFTHHQKIVVVDGEMPSGESQMRRIVSFVGGIDL 360

Query: 361 CDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLY 420
           CDGRYDTPFHSLFRTLD+AHHDDFHQPNF G+SI KGGPREPWHDIHSRLEGPVAWDVL+
Sbjct: 361 CDGRYDTPFHSLFRTLDTAHHDDFHQPNFAGSSITKGGPREPWHDIHSRLEGPVAWDVLF 420

Query: 421 NFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSP 480
           NFEQRWR+QGGKD+LV LRELD I IPPSPVMFPDD ++WN Q+FRSIDGGAAFGFP++P
Sbjct: 421 NFEQRWRQQGGKDVLVNLRELDNIIIPPSPVMFPDDHETWNVQLFRSIDGGAAFGFPETP 480

Query: 481 EEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMIN 540
           EEAA+AGL+SGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSF+W   D+K E IN
Sbjct: 481 EEAARAGLVSGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFDWSSDDIKREDIN 540

Query: 541 ALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDI 600
           ALHLIPKELSLKIVSKIE  ERFTVYVVVPMWPEG PE  +VQAILDWQRRTMEMMYKDI
Sbjct: 541 ALHLIPKELSLKIVSKIERGERFTVYVVVPMWPEGVPESASVQAILDWQRRTMEMMYKDI 600

Query: 601 ALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHA 660
             ALR    EEDPRNYLTFFC+GNREVKK GEYEPSE P+++S Y+RAQ+ARRFMIYVH 
Sbjct: 601 IQALRAKDREEDPRNYLTFFCLGNREVKKSGEYEPSERPEDDSDYIRAQEARRFMIYVHT 660

Query: 661 KMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFE 720
           KMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQP+HL+  +PARGQ+HGFRMALW+E
Sbjct: 661 KMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPYHLTINQPARGQIHGFRMALWYE 720

Query: 721 HLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITE 780
           HLG+L D+FL PE+IECV+KVN++A KYWDLY+SE  EHDLPGHLLRYPI +S +G++TE
Sbjct: 721 HLGMLDDTFLEPENIECVQKVNRVAGKYWDLYASELLEHDLPGHLLRYPIGVSSEGDVTE 780

Query: 781 LPGFEFFPDTKARVLGNKSNYLPPILTT 808
           LPG EFFPDTKARVLG KS+YLPPILTT
Sbjct: 781 LPGTEFFPDTKARVLGAKSDYLPPILTT 808

BLAST of Cp4.1LG02g14730 vs. ExPASy Swiss-Prot
Match: O04865 (Phospholipase D alpha 1 OS=Vigna unguiculata OX=3917 GN=PLD1 PE=2 SV=1)

HSP 1 Score: 1375.1 bits (3558), Expect = 0.0e+00
Identity = 629/809 (77.75%), Postives = 715/809 (88.38%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLH-SGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARV 60
           MA ILLHGTLH TIYEVD+LH  G  NF   L +N+EE VG G+GVT+LYATIDLEKARV
Sbjct: 1   MAQILLHGTLHATIYEVDELHGGGGGNFFSKLKQNIEETVGIGKGVTKLYATIDLEKARV 60

Query: 61  GRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEE 120
           GRTR +E E +NPKW E+FHIYC H+ASNIIFTVKDDNPIGATLIGRAY+PV E++ G E
Sbjct: 61  GRTRIIENETTNPKWNESFHIYCGHLASNIIFTVKDDNPIGATLIGRAYVPVSEVLDGHE 120

Query: 121 VDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCK 180
           +D+WV ILD  K PI G  KIHV+LQYFDV KD+NW RGI+S K+PGVPYT+FSQRQGCK
Sbjct: 121 IDKWVEILDTEKNPIEGGSKIHVRLQYFDVLKDRNWARGIRSPKYPGVPYTFFSQRQGCK 180

Query: 181 VTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVR 240
           V LYQDAHVPDNF+PKIPLAGGK YE +RCWEDIFDAI NAKH+IYITGWSVYTEISL+R
Sbjct: 181 VFLYQDAHVPDNFVPKIPLAGGKNYEAHRCWEDIFDAITNAKHLIYITGWSVYTEISLIR 240

Query: 241 DKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFAN 300
           D RRPK GGD  +GELLKKKA+EGVRVL+LVW+D TSV +LK+DGLMATHDE+T +FF +
Sbjct: 241 DSRRPKAGGDQTIGELLKKKASEGVRVLMLVWDDRTSVGLLKKDGLMATHDEETEQFFRD 300

Query: 301 TDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALP-NGDPNRRRIVSFVGGID 360
           TDVHCVLCPRNPDDG +++QD+ + TMFTHHQKIVVVDSALP  G  ++RRIVSFVGG+D
Sbjct: 301 TDVHCVLCPRNPDDGGSIVQDLQISTMFTHHQKIVVVDSALPGGGGSDKRRIVSFVGGLD 360

Query: 361 LCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVL 420
           LCDGRYDT FHSLFRTLD+AHHDDFHQPNF G +I KGGPREPWHDIHSR+EGP+AWDVL
Sbjct: 361 LCDGRYDTAFHSLFRTLDTAHHDDFHQPNFPGAAITKGGPREPWHDIHSRVEGPIAWDVL 420

Query: 421 YNFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDS 480
           +NFEQRWRKQGGKD+L  LREL+ + IPPSPV FPDD ++WN Q+FRSIDGGAAFGFPD+
Sbjct: 421 FNFEQRWRKQGGKDILAPLRELEDVIIPPSPVTFPDDHETWNVQLFRSIDGGAAFGFPDT 480

Query: 481 PEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMI 540
           PE+AAKAGL+SGKDNIIDRSIQDAYI+AIRRAKNFIYIENQYFLGSSF W   D+K E I
Sbjct: 481 PEDAAKAGLVSGKDNIIDRSIQDAYIHAIRRAKNFIYIENQYFLGSSFSWNNDDIKREEI 540

Query: 541 NALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKD 600
            ALHLIPKELSLKIVSKIEA ERF VYVVVPMWPEG PE  +VQAILDWQ+RT+EMMYKD
Sbjct: 541 GALHLIPKELSLKIVSKIEAGERFAVYVVVPMWPEGIPESSSVQAILDWQKRTIEMMYKD 600

Query: 601 IALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVH 660
           +  ALR  G +EDPRNYLTFFC+GNREVKK GEYEP+E P+ +S Y RAQ+ARRFMIYVH
Sbjct: 601 VVQALRAKGSDEDPRNYLTFFCLGNREVKKSGEYEPAEQPEPDSDYQRAQEARRFMIYVH 660

Query: 661 AKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWF 720
            KMMIVDDEYII+GSANINQRSMDGARDSEIAMG YQP+HL++ +PARGQV+GFRM+LW+
Sbjct: 661 TKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLANTQPARGQVYGFRMSLWY 720

Query: 721 EHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEIT 780
           EHLG+LHD+F  PES EC+ KVNQ+ADKYWDLYSSE+ E DLPGHLLRYPI ++ +GE+T
Sbjct: 721 EHLGMLHDTFQRPESEECINKVNQIADKYWDLYSSESLERDLPGHLLRYPIGVASEGEVT 780

Query: 781 ELPGFEFFPDTKARVLGNKSNYLPPILTT 808
           ELPGFEFFPDTKAR+LG K++YLPPILTT
Sbjct: 781 ELPGFEFFPDTKARILGAKADYLPPILTT 809

BLAST of Cp4.1LG02g14730 vs. ExPASy Swiss-Prot
Match: Q38882 (Phospholipase D alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA1 PE=1 SV=2)

HSP 1 Score: 1363.6 bits (3528), Expect = 0.0e+00
Identity = 622/810 (76.79%), Postives = 712/810 (87.90%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGA--RNFLKSLVENVEEAVGFGRGVTRLYATIDLEKAR 60
           MA  LLHGTLH TIYEVD LH G   + FL  ++ NVEE +G G+G T+LYATIDL+KAR
Sbjct: 1   MAQHLLHGTLHATIYEVDALHGGGVRQGFLGKILANVEETIGVGKGETQLYATIDLQKAR 60

Query: 61  VGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGE 120
           VGRTR+++ E  NPKWYE+FHIYCAH+AS+IIFTVKDDNPIGATLIGRAY+PV ++I+GE
Sbjct: 61  VGRTRKIKNEPKNPKWYESFHIYCAHLASDIIFTVKDDNPIGATLIGRAYIPVDQVINGE 120

Query: 121 EVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGC 180
           EVDQWV ILD  + PI+G  KIHVKLQYF V +D+NW  GIKS+KFPGVPYT+FSQRQGC
Sbjct: 121 EVDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKSAKFPGVPYTFFSQRQGC 180

Query: 181 KVTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLV 240
           KV+LYQDAH+PDNF+P+IPLAGGK YEP RCWEDIFDAI NAKH+IYITGWSVY EI+LV
Sbjct: 181 KVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAKHLIYITGWSVYAEIALV 240

Query: 241 RDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFA 300
           RD RRPKPGGD+ +GELLKKKA+EGVRVLLLVW+D TSV +LK+DGLMATHDE+T  FF 
Sbjct: 241 RDSRRPKPGGDVTIGELLKKKASEGVRVLLLVWDDRTSVDVLKKDGLMATHDEETENFFR 300

Query: 301 NTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPN-GDPNRRRIVSFVGGI 360
            +DVHC+LCPRNPDDG +++Q + + TMFTHHQKIVVVDS +P+ G    RRIVSFVGGI
Sbjct: 301 GSDVHCILCPRNPDDGGSIVQSLQISTMFTHHQKIVVVDSEMPSRGGSEMRRIVSFVGGI 360

Query: 361 DLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDV 420
           DLCDGRYDTPFHSLFRTLD+ HHDDFHQPNFTG +I KGGPREPWHDIHSRLEGP+AWDV
Sbjct: 361 DLCDGRYDTPFHSLFRTLDTVHHDDFHQPNFTGAAITKGGPREPWHDIHSRLEGPIAWDV 420

Query: 421 LYNFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPD 480
           +YNFEQRW KQGGKD+LVKLR+L  I I PSPVMF +D D WN Q+FRSIDGGAA GFP+
Sbjct: 421 MYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHDVWNVQLFRSIDGGAAAGFPE 480

Query: 481 SPEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEM 540
           SPE AA+AGL+SGKDNIIDRSIQDAYI+AIRRAK+FIY+ENQYFLGSSF W    + PE 
Sbjct: 481 SPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVENQYFLGSSFAWAADGITPED 540

Query: 541 INALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYK 600
           INALHLIPKELSLKIVSKIE  E+F VYVVVPMWPEG PE G+VQAILDWQRRTMEMMYK
Sbjct: 541 INALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPESGSVQAILDWQRRTMEMMYK 600

Query: 601 DIALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYV 660
           D+  ALR  G+EEDPRNYLTFFC+GNREVKK GEYEP+E PD ++ Y+RAQ+ARRFMIYV
Sbjct: 601 DVIQALRAQGLEEDPRNYLTFFCLGNREVKKDGEYEPAEKPDPDTDYMRAQEARRFMIYV 660

Query: 661 HAKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALW 720
           H KMMIVDDEYII+GSANINQRSMDGARDSEIAMG YQP HLS R+PARGQ+HGFRM+LW
Sbjct: 661 HTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQIHGFRMSLW 720

Query: 721 FEHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEI 780
           +EHLG+L ++FL P S+EC+ KVN+++DKYWD YSSE+ EHDLPGHLLRYPI ++ +G+I
Sbjct: 721 YEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLEHDLPGHLLRYPIGVASEGDI 780

Query: 781 TELPGFEFFPDTKARVLGNKSNYLPPILTT 808
           TELPGFEFFPDTKAR+LG KS+YLPPILTT
Sbjct: 781 TELPGFEFFPDTKARILGTKSDYLPPILTT 810

BLAST of Cp4.1LG02g14730 vs. ExPASy Swiss-Prot
Match: P55939 (Phospholipase D alpha 2 OS=Brassica oleracea var. capitata OX=3716 GN=PLD2 PE=1 SV=2)

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 628/812 (77.34%), Postives = 710/812 (87.44%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSG---ARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKA 60
           MA  LLHGTLH TIYEVD LH+G   +  FL  ++ NVEE +GFG+G T+LYATIDL+KA
Sbjct: 1   MAQHLLHGTLHATIYEVDALHTGGLRSAGFLGKIISNVEETIGFGKGETQLYATIDLQKA 60

Query: 61  RVGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISG 120
           RVGRTR++  E  NPKWYE+FHIYCAHMAS+IIFTVKDDNPIGATLIGRAY+PV E+I+G
Sbjct: 61  RVGRTRKITDEPKNPKWYESFHIYCAHMASDIIFTVKDDNPIGATLIGRAYVPVDEVING 120

Query: 121 EEVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQG 180
           EEV++WV ILD+ + PI G+ KIHVKLQYF V  D+NW  G+KS+KFPGVPYT+FSQRQG
Sbjct: 121 EEVEKWVEILDDDRNPIHGESKIHVKLQYFAVEADRNWNMGVKSAKFPGVPYTFFSQRQG 180

Query: 181 CKVTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISL 240
           CKV+LYQ AHVPDNF+PKIPLAGGK YEP+RCWEDIFDAI NAKH+IYITGWSVYTEI+L
Sbjct: 181 CKVSLYQGAHVPDNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKHLIYITGWSVYTEITL 240

Query: 241 VRDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFF 300
           VRD RRPKPGGDM LGELLKKKA EGVRVLLLVW+D TSV +LK+DGLMATHDEDT  +F
Sbjct: 241 VRDSRRPKPGGDMTLGELLKKKATEGVRVLLLVWDDRTSVDVLKKDGLMATHDEDTENYF 300

Query: 301 ANTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPN--GDPNRRRIVSFVG 360
             ++VHCVLCPRNPDDG +++Q++ V  MFTHHQKIVVVDS +P+  G    RRI+SFVG
Sbjct: 301 NGSEVHCVLCPRNPDDGGSIVQNLQVSAMFTHHQKIVVVDSEVPSQGGGSEMRRIMSFVG 360

Query: 361 GIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAW 420
           GIDLCDGRYDTPFHSLFRTLD+ HHDDFHQPNFTG SI KGGPREPW DIHSRLEGP+AW
Sbjct: 361 GIDLCDGRYDTPFHSLFRTLDTVHHDDFHQPNFTGASITKGGPREPWQDIHSRLEGPIAW 420

Query: 421 DVLYNFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGF 480
           DVLYNFEQRW KQGGKD+LVKLREL  I I PSPVMF +D D WN Q+FRSIDGGAA GF
Sbjct: 421 DVLYNFEQRWSKQGGKDILVKLRELSDIIITPSPVMFQEDHDVWNVQLFRSIDGGAAAGF 480

Query: 481 PDSPEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKP 540
           PDSPE AA+AGL+SGKDN+IDRSIQDAYI+AIRRAK+FIYIENQYFLGSSF W    + P
Sbjct: 481 PDSPEVAAEAGLVSGKDNVIDRSIQDAYIHAIRRAKDFIYIENQYFLGSSFAWAADGITP 540

Query: 541 EMINALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMM 600
           E INALHLIPKELSLKIV KIE  E+F VYVVVPMWPEG PE  +VQAILDWQRRT+EMM
Sbjct: 541 EDINALHLIPKELSLKIVDKIEKGEKFRVYVVVPMWPEGIPESASVQAILDWQRRTLEMM 600

Query: 601 YKDIALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMI 660
           YKD+  ALR  G+EEDPRNYLTFFC+GNREVKK GEYEP+E PD ++ Y+RAQ+ARRFMI
Sbjct: 601 YKDVTQALRAQGLEEDPRNYLTFFCLGNREVKKEGEYEPAERPDPDTDYMRAQEARRFMI 660

Query: 661 YVHAKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMA 720
           YVH+KMMIVDDEYIIVGSANINQRSMDGARDSEIAMG YQP HLS R+PARGQVHGFRM+
Sbjct: 661 YVHSKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQVHGFRMS 720

Query: 721 LWFEHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDG 780
           LW+EHLG+L ++FL P S+EC+ KVN++ADKYWD YSSE+ EHDLPGHLLRYPIS+  +G
Sbjct: 721 LWYEHLGMLDETFLDPSSLECIEKVNRIADKYWDFYSSESLEHDLPGHLLRYPISVDNEG 780

Query: 781 EITELPGFEFFPDTKARVLGNKSNYLPPILTT 808
            ITELPGFEFFPD+KAR+LGNK +YLPPILTT
Sbjct: 781 NITELPGFEFFPDSKARILGNKVDYLPPILTT 812

BLAST of Cp4.1LG02g14730 vs. NCBI nr
Match: XP_023523927.1 (phospholipase D alpha 1-like [Cucurbita pepo subsp. pepo] >XP_023523928.1 phospholipase D alpha 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1678 bits (4346), Expect = 0.0
Identity = 807/807 (100.00%), Postives = 807/807 (100.00%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK
Sbjct: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL
Sbjct: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGFEFFPDTKARVLGNKSNYLPPILTT
Sbjct: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807

BLAST of Cp4.1LG02g14730 vs. NCBI nr
Match: XP_022948622.1 (phospholipase D alpha 1-like [Cucurbita moschata] >XP_022948623.1 phospholipase D alpha 1-like [Cucurbita moschata])

HSP 1 Score: 1678 bits (4345), Expect = 0.0
Identity = 806/807 (99.88%), Postives = 807/807 (100.00%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLRELDAIFIPPSP+MFPDDFDSWNAQVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPIMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK
Sbjct: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL
Sbjct: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGFEFFPDTKARVLGNKSNYLPPILTT
Sbjct: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807

BLAST of Cp4.1LG02g14730 vs. NCBI nr
Match: KAG7036505.1 (Phospholipase D alpha 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1676 bits (4340), Expect = 0.0
Identity = 806/807 (99.88%), Postives = 806/807 (99.88%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK
Sbjct: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLHDSFLFPESIECV KVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL
Sbjct: 721 LGLLHDSFLFPESIECVTKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGFEFFPDTKARVLGNKSNYLPPILTT
Sbjct: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807

BLAST of Cp4.1LG02g14730 vs. NCBI nr
Match: XP_022998815.1 (phospholipase D alpha 1-like [Cucurbita maxima] >XP_022998816.1 phospholipase D alpha 1-like [Cucurbita maxima])

HSP 1 Score: 1664 bits (4310), Expect = 0.0
Identity = 799/807 (99.01%), Postives = 804/807 (99.63%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           DQWVPILDERKKPIRG+PKIHVKLQYFDVTKDQNW RGIKSSKFPGVPYTYFSQRQGCKV
Sbjct: 121 DQWVPILDERKKPIRGEPKIHVKLQYFDVTKDQNWARGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGD+MLGELLKKKA+EGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDVMLGELLKKKASEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DV CVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVQCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKIVSKIEARERFTVYVV+PMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVIPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK
Sbjct: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPI ISG+GEITEL
Sbjct: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPIGISGEGEITEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGFEFFPDTKARVLGNKSNYLPPILTT
Sbjct: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807

BLAST of Cp4.1LG02g14730 vs. NCBI nr
Match: KAG6606795.1 (Phospholipase D alpha 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1650 bits (4273), Expect = 0.0
Identity = 794/797 (99.62%), Postives = 794/797 (99.62%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK
Sbjct: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLHDSFLFPESIECV KVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL
Sbjct: 721 LGLLHDSFLFPESIECVTKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780

Query: 781 PGFEFFPDTKARVLGNK 797
           PGFEFFPDTKARVL  K
Sbjct: 781 PGFEFFPDTKARVLEKK 797

BLAST of Cp4.1LG02g14730 vs. ExPASy TrEMBL
Match: A0A6J1G9R5 (Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452246 PE=3 SV=1)

HSP 1 Score: 1678 bits (4345), Expect = 0.0
Identity = 806/807 (99.88%), Postives = 807/807 (100.00%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV
Sbjct: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLRELDAIFIPPSP+MFPDDFDSWNAQVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPIMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK
Sbjct: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL
Sbjct: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGFEFFPDTKARVLGNKSNYLPPILTT
Sbjct: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807

BLAST of Cp4.1LG02g14730 vs. ExPASy TrEMBL
Match: A0A6J1KB94 (Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111493365 PE=3 SV=1)

HSP 1 Score: 1664 bits (4310), Expect = 0.0
Identity = 799/807 (99.01%), Postives = 804/807 (99.63%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV
Sbjct: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           DQWVPILDERKKPIRG+PKIHVKLQYFDVTKDQNW RGIKSSKFPGVPYTYFSQRQGCKV
Sbjct: 121 DQWVPILDERKKPIRGEPKIHVKLQYFDVTKDQNWARGIKSSKFPGVPYTYFSQRQGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGD+MLGELLKKKA+EGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDVMLGELLKKKASEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DV CVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC
Sbjct: 301 DVQCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA
Sbjct: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKIVSKIEARERFTVYVV+PMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA
Sbjct: 541 LHLIPKELSLKIVSKIEARERFTVYVVIPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK
Sbjct: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPI ISG+GEITEL
Sbjct: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPIGISGEGEITEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGFEFFPDTKARVLGNKSNYLPPILTT
Sbjct: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807

BLAST of Cp4.1LG02g14730 vs. ExPASy TrEMBL
Match: A0A5D3E0E7 (Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001730 PE=3 SV=1)

HSP 1 Score: 1585 bits (4103), Expect = 0.0
Identity = 741/807 (91.82%), Postives = 784/807 (97.15%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSG RNFLK LVENVEEAVGFGRG+TRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGGRNFLKQLVENVEEAVGFGRGITRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLE+EHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPV+EII G+EV
Sbjct: 61  RTRRLEREHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVREIIRGDEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           D+WVPILDE+KKPIRG+PKIHVKLQ+FDVTKDQNWGRGIKS KFPGVPYTYFSQR+GCKV
Sbjct: 121 DKWVPILDEQKKPIRGEPKIHVKLQFFDVTKDQNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEP+RCWED+FDAI+NAKH+IYI+GWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPHRCWEDVFDAIMNAKHLIYISGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGD+MLGELLKKKA EGV VLLL+WNDTTSVP  KE+GLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDIMLGELLKKKAGEGVTVLLLIWNDTTSVPFFKEEGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDP++RRIVSFVGG+DLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPSKRRIVSFVGGLDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDVL+N
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFAGTSINKGGPREPWHDIHSRLEGPVAWDVLHN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLR+LD IFI PSPV FPDDFDSWN QVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRDLDQIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGL+SGKDN IDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRG DVKPE+INA
Sbjct: 481 EAAKAGLVSGKDNTIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEVINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKI+SKIEARERFTVY++VPMWPEGFPEGGTVQAILDWQRRTMEMMYKDI 
Sbjct: 541 LHLIPKELSLKIMSKIEARERFTVYILVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIV 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALR NG+EEDPRNYLTFFCVGNREVKKPGEYEPSESPDENS YLRAQQ+RRFMIYVH+K
Sbjct: 601 LALRANGVEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSDYLRAQQSRRFMIYVHSK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMG YQP+HLS+REPARGQ+HGFR+ALW+EH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGGYQPYHLSAREPARGQIHGFRLALWYEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLH SFLFPESIECV+ VNQLADKYWDLYSSETF+ DLPGHLLRYPI++S DG++TEL
Sbjct: 721 LGLLHQSFLFPESIECVKTVNQLADKYWDLYSSETFDRDLPGHLLRYPIAVSADGQVTEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGF+FFPDTKARVLGNKSNY+PPILTT
Sbjct: 781 PGFQFFPDTKARVLGNKSNYIPPILTT 807

BLAST of Cp4.1LG02g14730 vs. ExPASy TrEMBL
Match: A7XQW1 (Phospholipase D OS=Cucumis melo var. inodorus OX=357961 GN=PLDa2 PE=2 SV=1)

HSP 1 Score: 1585 bits (4103), Expect = 0.0
Identity = 741/807 (91.82%), Postives = 784/807 (97.15%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSG RNFLK LVENVEEAVGFGRG+TRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGGRNFLKQLVENVEEAVGFGRGITRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLE+EHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPV+EII G+EV
Sbjct: 61  RTRRLEREHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVREIIRGDEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           D+WVPILDE+KKPIRG+PKIHVKLQ+FDVTKDQNWGRGIKS KFPGVPYTYFSQR+GCKV
Sbjct: 121 DKWVPILDEQKKPIRGEPKIHVKLQFFDVTKDQNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEP+RCWED+FDAI+NAKH+IYI+GWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPHRCWEDVFDAIMNAKHLIYISGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGD+MLGELLKKKA EGV VLLL+WNDTTSVP  KE+GLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDIMLGELLKKKAGEGVTVLLLIWNDTTSVPFFKEEGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDP++RRIVSFVGG+DLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPSKRRIVSFVGGLDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDVL+N
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFAGTSINKGGPREPWHDIHSRLEGPVAWDVLHN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLR+LD IFI PSPV FPDDFDSWN QVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRDLDQIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGL+SGKDN IDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRG DVKPE+INA
Sbjct: 481 EAAKAGLVSGKDNTIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEVINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKI+SKIEARERFTVY++VPMWPEGFPEGGTVQAILDWQRRTMEMMYKDI 
Sbjct: 541 LHLIPKELSLKIMSKIEARERFTVYILVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIV 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALR NG+EEDPRNYLTFFCVGNREVKKPGEYEPSESPDENS YLRAQQ+RRFMIYVH+K
Sbjct: 601 LALRANGVEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSDYLRAQQSRRFMIYVHSK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMG YQP+HLS+REPARGQ+HGFR+ALW+EH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGGYQPYHLSAREPARGQIHGFRLALWYEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLH SFLFPESIECV+ VNQLADKYWDLYSSETF+ DLPGHLLRYPI++S DG++TEL
Sbjct: 721 LGLLHQSFLFPESIECVKTVNQLADKYWDLYSSETFDRDLPGHLLRYPIAVSADGQVTEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGF+FFPDTKARVLGNKSNY+PPILTT
Sbjct: 781 PGFQFFPDTKARVLGNKSNYIPPILTT 807

BLAST of Cp4.1LG02g14730 vs. ExPASy TrEMBL
Match: A0A1S3BY58 (Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103494704 PE=3 SV=1)

HSP 1 Score: 1585 bits (4103), Expect = 0.0
Identity = 741/807 (91.82%), Postives = 784/807 (97.15%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKARVG 60
           MAHILLHGTLHVTIYEVDKLHSG RNFLK LVENVEEAVGFGRG+TRLYATIDLEKARVG
Sbjct: 1   MAHILLHGTLHVTIYEVDKLHSGGRNFLKQLVENVEEAVGFGRGITRLYATIDLEKARVG 60

Query: 61  RTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGEEV 120
           RTRRLE+EHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPV+EII G+EV
Sbjct: 61  RTRRLEREHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVREIIRGDEV 120

Query: 121 DQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGCKV 180
           D+WVPILDE+KKPIRG+PKIHVKLQ+FDVTKDQNWGRGIKS KFPGVPYTYFSQR+GCKV
Sbjct: 121 DKWVPILDEQKKPIRGEPKIHVKLQFFDVTKDQNWGRGIKSPKFPGVPYTYFSQRRGCKV 180

Query: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLVRD 240
           TLYQDAHVPDNFIPKIPLAGGKFYEP+RCWED+FDAI+NAKH+IYI+GWSVYTEISLVRD
Sbjct: 181 TLYQDAHVPDNFIPKIPLAGGKFYEPHRCWEDVFDAIMNAKHLIYISGWSVYTEISLVRD 240

Query: 241 KRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFANT 300
           KRRPKPGGD+MLGELLKKKA EGV VLLL+WNDTTSVP  KE+GLMATHDEDTAKFFANT
Sbjct: 241 KRRPKPGGDIMLGELLKKKAGEGVTVLLLIWNDTTSVPFFKEEGLMATHDEDTAKFFANT 300

Query: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGIDLC 360
           DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDP++RRIVSFVGG+DLC
Sbjct: 301 DVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPSKRRIVSFVGGLDLC 360

Query: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVLYN 420
           DGRYDTPFHSLFRTLDSAHHDDFHQPNF GTSINKGGPREPWHDIHSRLEGPVAWDVL+N
Sbjct: 361 DGRYDTPFHSLFRTLDSAHHDDFHQPNFAGTSINKGGPREPWHDIHSRLEGPVAWDVLHN 420

Query: 421 FEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDSPE 480
           FEQRWRKQGGKDLLVKLR+LD IFI PSPV FPDDFDSWN QVFRSIDGGAAFGFPDSPE
Sbjct: 421 FEQRWRKQGGKDLLVKLRDLDQIFITPSPVTFPDDFDSWNVQVFRSIDGGAAFGFPDSPE 480

Query: 481 EAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMINA 540
           EAAKAGL+SGKDN IDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRG DVKPE+INA
Sbjct: 481 EAAKAGLVSGKDNTIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGDDVKPEVINA 540

Query: 541 LHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIA 600
           LHLIPKELSLKI+SKIEARERFTVY++VPMWPEGFPEGGTVQAILDWQRRTMEMMYKDI 
Sbjct: 541 LHLIPKELSLKIMSKIEARERFTVYILVPMWPEGFPEGGTVQAILDWQRRTMEMMYKDIV 600

Query: 601 LALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYVHAK 660
           LALR NG+EEDPRNYLTFFCVGNREVKKPGEYEPSESPDENS YLRAQQ+RRFMIYVH+K
Sbjct: 601 LALRANGVEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSDYLRAQQSRRFMIYVHSK 660

Query: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALWFEH 720
           MMIVDDEYIIVGSANINQRSMDGARDSEIAMG YQP+HLS+REPARGQ+HGFR+ALW+EH
Sbjct: 661 MMIVDDEYIIVGSANINQRSMDGARDSEIAMGGYQPYHLSAREPARGQIHGFRLALWYEH 720

Query: 721 LGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEITEL 780
           LGLLH SFLFPESIECV+ VNQLADKYWDLYSSETF+ DLPGHLLRYPI++S DG++TEL
Sbjct: 721 LGLLHQSFLFPESIECVKTVNQLADKYWDLYSSETFDRDLPGHLLRYPIAVSADGQVTEL 780

Query: 781 PGFEFFPDTKARVLGNKSNYLPPILTT 807
           PGF+FFPDTKARVLGNKSNY+PPILTT
Sbjct: 781 PGFQFFPDTKARVLGNKSNYIPPILTT 807

BLAST of Cp4.1LG02g14730 vs. TAIR 10
Match: AT3G15730.1 (phospholipase D alpha 1 )

HSP 1 Score: 1363.6 bits (3528), Expect = 0.0e+00
Identity = 622/810 (76.79%), Postives = 712/810 (87.90%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHSGA--RNFLKSLVENVEEAVGFGRGVTRLYATIDLEKAR 60
           MA  LLHGTLH TIYEVD LH G   + FL  ++ NVEE +G G+G T+LYATIDL+KAR
Sbjct: 1   MAQHLLHGTLHATIYEVDALHGGGVRQGFLGKILANVEETIGVGKGETQLYATIDLQKAR 60

Query: 61  VGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGE 120
           VGRTR+++ E  NPKWYE+FHIYCAH+AS+IIFTVKDDNPIGATLIGRAY+PV ++I+GE
Sbjct: 61  VGRTRKIKNEPKNPKWYESFHIYCAHLASDIIFTVKDDNPIGATLIGRAYIPVDQVINGE 120

Query: 121 EVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGC 180
           EVDQWV ILD  + PI+G  KIHVKLQYF V +D+NW  GIKS+KFPGVPYT+FSQRQGC
Sbjct: 121 EVDQWVEILDNDRNPIQGGSKIHVKLQYFHVEEDRNWNMGIKSAKFPGVPYTFFSQRQGC 180

Query: 181 KVTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLV 240
           KV+LYQDAH+PDNF+P+IPLAGGK YEP RCWEDIFDAI NAKH+IYITGWSVY EI+LV
Sbjct: 181 KVSLYQDAHIPDNFVPRIPLAGGKNYEPQRCWEDIFDAISNAKHLIYITGWSVYAEIALV 240

Query: 241 RDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFA 300
           RD RRPKPGGD+ +GELLKKKA+EGVRVLLLVW+D TSV +LK+DGLMATHDE+T  FF 
Sbjct: 241 RDSRRPKPGGDVTIGELLKKKASEGVRVLLLVWDDRTSVDVLKKDGLMATHDEETENFFR 300

Query: 301 NTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPN-GDPNRRRIVSFVGGI 360
            +DVHC+LCPRNPDDG +++Q + + TMFTHHQKIVVVDS +P+ G    RRIVSFVGGI
Sbjct: 301 GSDVHCILCPRNPDDGGSIVQSLQISTMFTHHQKIVVVDSEMPSRGGSEMRRIVSFVGGI 360

Query: 361 DLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDV 420
           DLCDGRYDTPFHSLFRTLD+ HHDDFHQPNFTG +I KGGPREPWHDIHSRLEGP+AWDV
Sbjct: 361 DLCDGRYDTPFHSLFRTLDTVHHDDFHQPNFTGAAITKGGPREPWHDIHSRLEGPIAWDV 420

Query: 421 LYNFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPD 480
           +YNFEQRW KQGGKD+LVKLR+L  I I PSPVMF +D D WN Q+FRSIDGGAA GFP+
Sbjct: 421 MYNFEQRWSKQGGKDILVKLRDLSDIIITPSPVMFQEDHDVWNVQLFRSIDGGAAAGFPE 480

Query: 481 SPEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEM 540
           SPE AA+AGL+SGKDNIIDRSIQDAYI+AIRRAK+FIY+ENQYFLGSSF W    + PE 
Sbjct: 481 SPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYVENQYFLGSSFAWAADGITPED 540

Query: 541 INALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYK 600
           INALHLIPKELSLKIVSKIE  E+F VYVVVPMWPEG PE G+VQAILDWQRRTMEMMYK
Sbjct: 541 INALHLIPKELSLKIVSKIEKGEKFRVYVVVPMWPEGLPESGSVQAILDWQRRTMEMMYK 600

Query: 601 DIALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYV 660
           D+  ALR  G+EEDPRNYLTFFC+GNREVKK GEYEP+E PD ++ Y+RAQ+ARRFMIYV
Sbjct: 601 DVIQALRAQGLEEDPRNYLTFFCLGNREVKKDGEYEPAEKPDPDTDYMRAQEARRFMIYV 660

Query: 661 HAKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALW 720
           H KMMIVDDEYII+GSANINQRSMDGARDSEIAMG YQP HLS R+PARGQ+HGFRM+LW
Sbjct: 661 HTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPHHLSHRQPARGQIHGFRMSLW 720

Query: 721 FEHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEI 780
           +EHLG+L ++FL P S+EC+ KVN+++DKYWD YSSE+ EHDLPGHLLRYPI ++ +G+I
Sbjct: 721 YEHLGMLDETFLDPSSLECIEKVNRISDKYWDFYSSESLEHDLPGHLLRYPIGVASEGDI 780

Query: 781 TELPGFEFFPDTKARVLGNKSNYLPPILTT 808
           TELPGFEFFPDTKAR+LG KS+YLPPILTT
Sbjct: 781 TELPGFEFFPDTKARILGTKSDYLPPILTT 810

BLAST of Cp4.1LG02g14730 vs. TAIR 10
Match: AT1G52570.1 (phospholipase D alpha 2 )

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 620/810 (76.54%), Postives = 713/810 (88.02%), Query Frame = 0

Query: 1   MAHILLHGTLHVTIYEVDKLHS--GARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKAR 60
           M   LLHG LH TIYEVD LH+  G   FL S++ NVEE +G G+G T+LYATIDLEKAR
Sbjct: 1   MEECLLHGRLHATIYEVDHLHAEGGRSGFLGSILANVEETIGVGKGETQLYATIDLEKAR 60

Query: 61  VGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISGE 120
           VGRTR++ KE  NPKW+E+FHIYC HMA ++IFTVKD NPIGATLIGR Y+PV++I+ GE
Sbjct: 61  VGRTRKITKEPKNPKWFESFHIYCGHMAKHVIFTVKDANPIGATLIGRGYIPVEDILHGE 120

Query: 121 EVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPGVPYTYFSQRQGC 180
           EVD+WV ILD  K PI G  KIHVKLQYF V KD+NW RGIKS+KFPGVPYT+FSQR+GC
Sbjct: 121 EVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSAKFPGVPYTFFSQRRGC 180

Query: 181 KVTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEISLV 240
           KV+LYQDAH+P NF+PKIPLAGGK YEP+RCWEDIFDAI NAKH+IYITGWSVYTEISLV
Sbjct: 181 KVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKHLIYITGWSVYTEISLV 240

Query: 241 RDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGLMATHDEDTAKFFA 300
           RD RRPK GGD+ +GELLKKKA+EGV+V+LLVW+D TSV +LK+DGLMATHDE+T  FF 
Sbjct: 241 RDSRRPKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLLKKDGLMATHDEETENFFR 300

Query: 301 NTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFVGGID 360
            TDV+C+LCPRNPDDG +++Q++ + TMFTHHQKIVVVDS +P+G    RRIVSFVGG+D
Sbjct: 301 GTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEMPSGGSRSRRIVSFVGGLD 360

Query: 361 LCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVAWDVL 420
           LCDGRYDTPFHSLFRTLD+AHHDDFHQPNFTG +I KGGPREPWHDIH RLEGP+AWDVL
Sbjct: 361 LCDGRYDTPFHSLFRTLDTAHHDDFHQPNFTGAAITKGGPREPWHDIHCRLEGPIAWDVL 420

Query: 421 YNFEQRWRKQGGKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVFRSIDGGAAFGFPDS 480
           YNFEQRW +QGGKD+LVK+REL  I IPPSPV+F +D D WN Q+FRSIDGGAA GFPDS
Sbjct: 421 YNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFSEDHDVWNVQLFRSIDGGAAAGFPDS 480

Query: 481 PEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGSSFEWRGTDVKPEMI 540
           PE AA+AGL+SGKDNIIDRSIQDAYI+AIRRAK+FIYIENQYFLGSSF W    +KPE I
Sbjct: 481 PEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIENQYFLGSSFAWSADGIKPEEI 540

Query: 541 NALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAILDWQRRTMEMMYKD 600
           NALHLIPKELSLKIVSKI+A E+F VYVVVPMWPEG PE G+VQAILDWQ+RTMEMMYKD
Sbjct: 541 NALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPESGSVQAILDWQKRTMEMMYKD 600

Query: 601 IALALRVNGIE-EDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSYLRAQQARRFMIYV 660
           +  ALR NG+E EDPR+YLTFFC+GNREVKK GEYEPSE P+ ++ Y+RAQ+ARRFMIYV
Sbjct: 601 VIKALRENGLEGEDPRDYLTFFCLGNREVKKDGEYEPSEKPEPDTDYIRAQEARRFMIYV 660

Query: 661 HAKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREPARGQVHGFRMALW 720
           H KMMIVDDEYII+GSANINQRSMDGARDSEIAMG YQP+HLS+R+PARGQ+HGFRM+LW
Sbjct: 661 HTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLSTRQPARGQIHGFRMSLW 720

Query: 721 FEHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFEHDLPGHLLRYPISISGDGEI 780
           +EHLG+L ++FL P S EC++KVN++ADKYWDLYSSE+ EHDLPGHLLRYPI I+ +G I
Sbjct: 721 YEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESLEHDLPGHLLRYPIGIASEGNI 780

Query: 781 TELPGFEFFPDTKARVLGNKSNYLPPILTT 808
           TELPG EFFPDTKAR+LG KS+Y+PPILTT
Sbjct: 781 TELPGCEFFPDTKARILGVKSDYMPPILTT 810

BLAST of Cp4.1LG02g14730 vs. TAIR 10
Match: AT5G25370.1 (phospholipase D alpha 3 )

HSP 1 Score: 1052.4 bits (2720), Expect = 1.9e-307
Identity = 504/827 (60.94%), Postives = 621/827 (75.09%), Query Frame = 0

Query: 4   ILLHGTLHVTIYEVDKLHSGAR-----------------NFLKSLVENVEEAVGFGRGVT 63
           +LLHGTL V IY +DKLH  +R                 + +K L ++     G      
Sbjct: 5   LLLHGTLEVKIYRIDKLHQRSRFNLCGKGNKEPTGKKTQSQIKRLTDSCTSLFG-----G 64

Query: 64  RLYATIDLEKARVGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGR 123
            LYATIDL+++RV RT  + + H  PKW ++FH+Y AH  S IIFTVK+D P+ A+LIGR
Sbjct: 65  HLYATIDLDRSRVART--MMRRH--PKWLQSFHVYTAHSISKIIFTVKEDEPVSASLIGR 124

Query: 124 AYLPVKEIISGEEVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSKFPG 183
           AYLPV E+I+G+ +D+W+ ILDE ++PI+G  K+HV++++  VT+D NW +GI    F G
Sbjct: 125 AYLPVTEVITGQPIDRWLDILDENRRPIQGGSKLHVRVKFTHVTQDVNWNKGIILPSFNG 184

Query: 184 VPYTYFSQRQGCKVTLYQDAHVPDNFIPKIPLAGGK-FYEPNRCWEDIFDAIINAKHMIY 243
           VP  YF+QR+GCKVTLYQDAHV + + P + L GG+  Y+ +RCWE+IFDAI  AKH+IY
Sbjct: 185 VPNAYFNQREGCKVTLYQDAHVLNEY-PDVTLTGGQVIYKHHRCWEEIFDAIWEAKHLIY 244

Query: 244 ITGWSVYTEISLVRDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPILKEDGL 303
           I GWSV T+++LVRD +R +PGGD+ LGELLKKKA E V VL+LVW+D TS  + K DGL
Sbjct: 245 IAGWSVNTDVTLVRDPKRTRPGGDLKLGELLKKKAEENVTVLMLVWDDRTSHEVFKRDGL 304

Query: 304 MATHDEDTAKFFANTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDP 363
           M THD++T  +F NT V CVLCPRNPD+G +++Q   V TMFTHHQK +VVDS + +G  
Sbjct: 305 MMTHDQETYDYFKNTKVRCVLCPRNPDNGDSIVQGFEVATMFTHHQKTIVVDSEV-DGSL 364

Query: 364 NRRRIVSFVGGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDI 423
            +RRIVSF+GGIDLCDGRYDT  H LF TL+S H +DFHQPNF G SI KGGPREPWHDI
Sbjct: 365 TKRRIVSFLGGIDLCDGRYDTVEHPLFGTLNSVHANDFHQPNFDGASIKKGGPREPWHDI 424

Query: 424 HSRLEGPVAWDVLYNFEQRWRKQG-GKDLLVKLRELDAIFIPPSPVMFPDDFDSWNAQVF 483
           H +L+GP AWDVLYNFEQRW KQG G+  L+ + +L  I +PP P++ PD+ + W  QVF
Sbjct: 425 HCKLDGPAAWDVLYNFEQRWMKQGSGRRYLISMAQLAEITVPPLPIVQPDNEEGWTVQVF 484

Query: 484 RSIDGGAAFGFPDSPEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFLGS 543
           RSID GA  GFP+ P EAA  GLISGKDN+I+RSIQDAY+NAIRRAKNFIYIENQYFLGS
Sbjct: 485 RSIDDGAVEGFPEDPREAASIGLISGKDNVIERSIQDAYVNAIRRAKNFIYIENQYFLGS 544

Query: 544 SFEWRGTDVKPEMINALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQAI 603
           SF W   D+    INAL LIPKE+SLKIVSKIEA ERF+VY+V+P+WPEG P   +VQAI
Sbjct: 545 SFGWNSRDINLNEINALQLIPKEISLKIVSKIEAGERFSVYIVIPLWPEGKPGSASVQAI 604

Query: 604 LDWQRRTMEMMYKDIALALRVNGIEEDPRNYLTFFCVGNREVKKPGEYEPSESPDENSSY 663
           LDWQRRTMEMMY DI +ALR  G++ +PR+YLTFFC+GNRE  K GEY P E P+ NS Y
Sbjct: 605 LDWQRRTMEMMYTDIIIALRKKGLDANPRDYLTFFCLGNREKGKVGEYLPPEKPEANSDY 664

Query: 664 LRAQQARRFMIYVHAKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHLSSREP 723
            RAQ++RRFMIYVH+KMMIVDDEYII+GSANINQRSMDG RD+EIAMGAYQP HL S   
Sbjct: 665 ARAQESRRFMIYVHSKMMIVDDEYIIIGSANINQRSMDGGRDTEIAMGAYQPSHLLSTNN 724

Query: 724 AR--GQVHGFRMALWFEHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETF--EHDL 783
            R  GQ+  FR++LW EHL +  ++F  PES EC+R VN  AD+ W LYS++ +    DL
Sbjct: 725 MRPVGQIFSFRISLWLEHLRVTTNAFQCPESEECIRMVNATADELWGLYSAQEYPRNDDL 784

Query: 784 PGHLLRYPISISGDGEITELPGFEFFPDTKARVLGNKSNYLPPILTT 808
           PGHLL YPISI  +GE+T L G EFFPDT A+V+G KSNYLPPILT+
Sbjct: 785 PGHLLSYPISIGSNGEVTNLAGTEFFPDTNAKVVGEKSNYLPPILTS 820

BLAST of Cp4.1LG02g14730 vs. TAIR 10
Match: AT2G42010.1 (phospholipase D beta 1 )

HSP 1 Score: 730.3 bits (1884), Expect = 1.7e-210
Identity = 367/819 (44.81%), Postives = 523/819 (63.86%), Query Frame = 0

Query: 4    ILLHGTLHVTIY------EVDKLHSGARNFLKSLVENVEEAVGFGRGVTRLYATIDLEKA 63
            +LLHG L + IY       +D  H    +    L   +E  +   +  +  Y ++ +  A
Sbjct: 269  LLLHGNLDIWIYHAKNLPNMDMFHKTLGDMFGRLPGKIEGQL-TSKITSDPYVSVSVAGA 328

Query: 64   RVGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAYLPVKEIISG 123
             +GRT  +     NP W + F++  AH A+ + F VKD + +G+ LIG   +PV++I SG
Sbjct: 329  VIGRTYVMSNS-ENPVWMQHFYVPVAHHAAEVHFVVKDSDVVGSQLIGLVTIPVEQIYSG 388

Query: 124  EEVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSS-KFPGVPYTYFSQRQ 183
             +++   PIL+   KP +    + + +QY  + K   +  G+ +   + GVP TYF  R+
Sbjct: 389  AKIEGTYPILNSNGKPCKPGANLSLSIQYTPMDKLSVYHHGVGAGPDYQGVPGTYFPLRK 448

Query: 184  GCKVTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYITGWSVYTEIS 243
            G  V LYQDAHVP+  +P I L  G  YE  +CW D+FDAI  A+ +IYITGWSV+ ++ 
Sbjct: 449  GGTVRLYQDAHVPEGMLPGIRLDNGMSYEHGKCWHDMFDAIRQARRLIYITGWSVWHKVK 508

Query: 244  LVRDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPIL--KEDGLMATHDEDTA 303
            L+RDK    P  +  LGELL+ K+ EGVRVLLL+W+D TS  IL  K DG+MATHDE+T 
Sbjct: 509  LIRDKL--GPASECTLGELLRSKSQEGVRVLLLIWDDPTSRSILGYKTDGVMATHDEETR 568

Query: 304  KFFANTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDPNRRRIVSFV 363
            +FF ++ V  +LCPRN     + ++   VGT++THHQK V+VD+   +   NRR+I++FV
Sbjct: 569  RFFKHSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNVIVDA---DAGGNRRKIIAFV 628

Query: 364  GGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDIHSRLEGPVA 423
            GG+DLCDGRYDTP H LFRTL + H DDFH P FTG     G PREPWHD+HS+++GP A
Sbjct: 629  GGLDLCDGRYDTPQHPLFRTLQTIHKDDFHNPTFTGNL--SGCPREPWHDLHSKIDGPAA 688

Query: 424  WDVLYNFEQRWRKQGG-----------KDLLVKLREL-DAIFIPPSPVMFPDDFDSWNAQ 483
            +DVL NFE+RW K               D L+++  + D + +  +P +  +D ++W+ Q
Sbjct: 689  YDVLTNFEERWLKAAKPSGIKKFKTSYDDALLRIDRIPDILGVSDTPTVSENDPEAWHVQ 748

Query: 484  VFRSIDGGAAFGFPDSPEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNFIYIENQYFL 543
            +FRSID  +  GFP  P++A    L+ GK+ +ID SI  AY+ AIR A++FIYIENQYF+
Sbjct: 749  IFRSIDSNSVKGFPKDPKDATCKNLVCGKNVLIDMSIHTAYVKAIRAAQHFIYIENQYFI 808

Query: 544  GSSFEWRGTDVKPEMINALHLIPKELSLKIVSKIEARERFTVYVVVPMWPEGFPEGGTVQ 603
            GSS+ W       + I A +LIP E++LKI  KI A ERF  Y+V+PMWPEG P G   Q
Sbjct: 809  GSSYNWNA----HKDIGANNLIPMEIALKIAEKIRANERFAAYIVIPMWPEGVPTGAATQ 868

Query: 604  AILDWQRRTMEMMYKDIALALRVNGIEE--DPRNYLTFFCVGNREVKKPGEYEPSESP-D 663
             IL WQ +T++MMY+ I  AL   G+E    P++YL FFC+GNRE+    +   + SP +
Sbjct: 869  RILYWQHKTIQMMYETIYKALVETGLEGAFSPQDYLNFFCLGNREMVDGIDNSGTGSPSN 928

Query: 664  ENSSYLRAQQARRFMIYVHAKMMIVDDEYIIVGSANINQRSMDGARDSEIAMGAYQPFHL 723
             N+    ++++RRFM+YVH+K M+VDDEY+++GSANINQRSM+G RD+EIAMGAYQP H 
Sbjct: 929  ANTPQALSRKSRRFMVYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEIAMGAYQPQHT 988

Query: 724  SSREPA--RGQVHGFRMALWFEHLGLLHDSFLFPESIECVRKVNQLADKYWDLYSSETFE 783
             +R+ +  RGQ++G+RM+LW EH+  L D F  PESIECVRKV  + ++ W  +++E   
Sbjct: 989  WARKHSGPRGQIYGYRMSLWAEHMATLDDCFTQPESIECVRKVRTMGERNWKQFAAEEVS 1048

Query: 784  HDLPGHLLRYPISISGDGEITELPGFEFFPDTKARVLGN 797
             D+ GHLL+YP+ +   G++  LPG E FPD    ++G+
Sbjct: 1049 -DMRGHLLKYPVEVDRKGKVRPLPGSETFPDVGGNIVGS 1073

BLAST of Cp4.1LG02g14730 vs. TAIR 10
Match: AT4G00240.1 (phospholipase D beta 2 )

HSP 1 Score: 719.5 bits (1856), Expect = 2.9e-207
Identity = 358/768 (46.61%), Postives = 497/768 (64.71%), Query Frame = 0

Query: 49  YATIDLEKARVGRTRRLEKEHSNPKWYETFHIYCAHMASNIIFTVKDDNPIGATLIGRAY 108
           Y +I +  A +GRT  +     NP W + F++  AH A+ + F VKD + +G+ LIG   
Sbjct: 163 YVSISVAGAVIGRTYVISNS-ENPVWQQHFYVPVAHHAAEVHFVVKDSDAVGSQLIGIVT 222

Query: 109 LPVKEIISGEEVDQWVPILDERKKPIRGDPKIHVKLQYFDVTKDQNWGRGIKSSK-FPGV 168
           +PV++I SG  ++    I D   KP +    + + +QY  + K   +  G+ +   + GV
Sbjct: 223 IPVEQIYSGARIEGTYSIRDSNGKPCKPGATLSLSIQYTSMNKLSVYHSGVGAGPYYQGV 282

Query: 169 PYTYFSQRQGCKVTLYQDAHVPDNFIPKIPLAGGKFYEPNRCWEDIFDAIINAKHMIYIT 228
           P TYF  R+G  VTLYQDAHVP+  +P I L  G  YE  +CW D+F AI  A+ +IYIT
Sbjct: 283 PGTYFPLREGGSVTLYQDAHVPEGMLPGIKLGNGMCYEHGKCWHDMFHAICQARRLIYIT 342

Query: 229 GWSVYTEISLVRDKRRPKPGGDMMLGELLKKKANEGVRVLLLVWNDTTSVPIL--KEDGL 288
           GWSV+  + LVRDK    P  +  LGELL+ K+ EGVRVLLLVW+D TS  IL    DG+
Sbjct: 343 GWSVWHNVRLVRDKE--DPSSECRLGELLRSKSQEGVRVLLLVWDDPTSQNILGYMTDGV 402

Query: 289 MATHDEDTAKFFANTDVHCVLCPRNPDDGANVIQDIAVGTMFTHHQKIVVVDSALPNGDP 348
           M THDE+T +FF ++ V  +LCPRN     + ++   VGT++THHQK ++VD+   +   
Sbjct: 403 MGTHDEETRRFFKDSSVQVLLCPRNAGKRHSWVKQREVGTIYTHHQKNLIVDA---DAGG 462

Query: 349 NRRRIVSFVGGIDLCDGRYDTPFHSLFRTLDSAHHDDFHQPNFTGTSINKGGPREPWHDI 408
           NRR+IV+FVGG+DLCDGRYDTP H LFRTL + H+ D+H P FTG     G PREPWHD+
Sbjct: 463 NRRKIVAFVGGLDLCDGRYDTPQHPLFRTLQTDHNGDYHNPTFTGNV--SGCPREPWHDL 522

Query: 409 HSRLEGPVAWDVLYNFEQRWRKQGGKDLLVKL------------RELDAIFIPPSPVMFP 468
           HS+++GP A+DVL NFE+RW K      + KL            R  D + +  +P +  
Sbjct: 523 HSKIDGPAAYDVLTNFEERWLKAAKPHRINKLKTSYDDALLRIDRIPDILRVLDAPTVSA 582

Query: 469 DDFDSWNAQVFRSIDGGAAFGFPDSPEEAAKAGLISGKDNIIDRSIQDAYINAIRRAKNF 528
           +D ++W+ Q+FRSID  +  GFP  P+ A    L+ GK+ +ID SI  AY+ AIR A++F
Sbjct: 583 NDPEAWHVQIFRSIDSNSVKGFPKDPKYATSKNLVCGKNVLIDMSIHTAYVKAIRAAQHF 642

Query: 529 IYIENQYFLGSSFEWRGTDVKPEMINALHLIPKELSLKIVSKIEARERFTVYVVVPMWPE 588
           IYIENQYF+GSS++W       + I A +LIP E++LKI  KI A+ERF  Y+V+PMWPE
Sbjct: 643 IYIENQYFIGSSYDWNA----HKDIGANNLIPMEIALKIADKIRAKERFAAYIVIPMWPE 702

Query: 589 GFPEGGTVQAILDWQRRTMEMMYKDIALALRVNGIEED--PRNYLTFFCVGNRE-VKKPG 648
           G P G   Q IL WQ +TM+MMY  I  AL   G+E++  P++YL FFC+GNRE V    
Sbjct: 703 GVPTGAATQRILYWQHKTMQMMYGTIYNALVEAGLEDEYSPQDYLNFFCLGNREMVNGNN 762

Query: 649 EYEPSESPDENSSYLRAQQARRFMIYVHAKMMIVDDEYIIVGSANINQRSMDGARDSEIA 708
           E     + +EN+     +++RRFMIYVH+K M+VDDEY+++GSANINQRSM+G RD+EIA
Sbjct: 763 ESGTGSASNENTPQGLCRKSRRFMIYVHSKGMVVDDEYVVIGSANINQRSMEGTRDTEIA 822

Query: 709 MGAYQPFHLSSREPA--RGQVHGFRMALWFEHLGLLHDSFLFPESIECVRKVNQLADKYW 768
           MGAYQP H  +R  +  RGQ++G+RM+LW EH+ LL D F+ PES+ CVRKV  +A++ W
Sbjct: 823 MGAYQPQHTWARRQSGPRGQIYGYRMSLWAEHMALLDDCFVEPESLGCVRKVRTVAEENW 882

Query: 769 DLYSSETFEHDLPGHLLRYPISISGDGEITELPGFEFFPDTKARVLGN 797
           + + SE    ++ GHL++YP+ +   G++  LPG E FPD    V+G+
Sbjct: 883 EQFRSEEVS-EMRGHLMKYPVEVDRKGKVRPLPGSEEFPDVGGNVVGS 917

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q411420.0e+0079.95Phospholipase D alpha 1 OS=Ricinus communis OX=3988 GN=PLD1 PE=1 SV=1[more]
P863870.0e+0078.34Phospholipase D alpha 1 OS=Carica papaya OX=3649 GN=PLD1 PE=1 SV=1[more]
O048650.0e+0077.75Phospholipase D alpha 1 OS=Vigna unguiculata OX=3917 GN=PLD1 PE=2 SV=1[more]
Q388820.0e+0076.79Phospholipase D alpha 1 OS=Arabidopsis thaliana OX=3702 GN=PLDALPHA1 PE=1 SV=2[more]
P559390.0e+0077.34Phospholipase D alpha 2 OS=Brassica oleracea var. capitata OX=3716 GN=PLD2 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_023523927.10.0100.00phospholipase D alpha 1-like [Cucurbita pepo subsp. pepo] >XP_023523928.1 phosph... [more]
XP_022948622.10.099.88phospholipase D alpha 1-like [Cucurbita moschata] >XP_022948623.1 phospholipase ... [more]
KAG7036505.10.099.88Phospholipase D alpha 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022998815.10.099.01phospholipase D alpha 1-like [Cucurbita maxima] >XP_022998816.1 phospholipase D ... [more]
KAG6606795.10.099.62Phospholipase D alpha 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1G9R50.099.88Phospholipase D OS=Cucurbita moschata OX=3662 GN=LOC111452246 PE=3 SV=1[more]
A0A6J1KB940.099.01Phospholipase D OS=Cucurbita maxima OX=3661 GN=LOC111493365 PE=3 SV=1[more]
A0A5D3E0E70.091.82Phospholipase D OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G0017... [more]
A7XQW10.091.82Phospholipase D OS=Cucumis melo var. inodorus OX=357961 GN=PLDa2 PE=2 SV=1[more]
A0A1S3BY580.091.82Phospholipase D OS=Cucumis melo OX=3656 GN=LOC103494704 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G15730.10.0e+0076.79phospholipase D alpha 1 [more]
AT1G52570.10.0e+0076.54phospholipase D alpha 2 [more]
AT5G25370.11.9e-30760.94phospholipase D alpha 3 [more]
AT2G42010.11.7e-21044.81phospholipase D beta 1 [more]
AT4G00240.12.9e-20746.61phospholipase D beta 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001736Phospholipase D/TransphosphatidylaseSMARTSM00155pld_4coord: 325..363
e-value: 0.0069
score: 25.6
coord: 653..680
e-value: 2.1E-8
score: 43.9
IPR001736Phospholipase D/TransphosphatidylasePFAMPF00614PLDccoord: 325..363
e-value: 3.2E-10
score: 39.7
coord: 654..680
e-value: 1.3E-7
score: 31.5
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 653..680
score: 15.197198
IPR001736Phospholipase D/TransphosphatidylasePROSITEPS50035PLDcoord: 325..363
score: 10.931199
IPR000008C2 domainSMARTSM00239C2_3ccoord: 9..123
e-value: 9.3E-13
score: 58.4
IPR000008C2 domainPFAMPF00168C2coord: 8..126
e-value: 2.2E-14
score: 53.6
IPR000008C2 domainPROSITEPS50004C2coord: 1..124
score: 14.576187
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 479..697
e-value: 4.6E-21
score: 77.6
NoneNo IPR availableGENE3D3.30.870.10Endonuclease Chain Acoord: 200..441
e-value: 1.3E-23
score: 85.2
NoneNo IPR availablePANTHERPTHR18896:SF162PHOSPHOLIPASE Dcoord: 2..807
NoneNo IPR availableCDDcd04015C2_plant_PLDcoord: 2..147
e-value: 4.19724E-66
score: 214.861
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 497..737
NoneNo IPR availableSUPERFAMILY56024Phospholipase D/nucleasecoord: 150..435
IPR024632Phospholipase D, C-terminalPFAMPF12357PLD_Ccoord: 726..797
e-value: 3.1E-29
score: 100.6
IPR011402Phospholipase D, plantPIRSFPIRSF036470PLD_plantcoord: 1..807
e-value: 0.0
score: 1271.4
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 6..157
e-value: 7.7E-15
score: 57.1
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 6..149
IPR015679Phospholipase D familyPANTHERPTHR18896PHOSPHOLIPASE Dcoord: 2..807

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g14730.1Cp4.1LG02g14730.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046470 phosphatidylcholine metabolic process
biological_process GO:0009395 phospholipid catabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0005886 plasma membrane
cellular_component GO:0009536 plastid
cellular_component GO:0005773 vacuole
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0070290 N-acylphosphatidylethanolamine-specific phospholipase D activity
molecular_function GO:0004630 phospholipase D activity
molecular_function GO:0003824 catalytic activity