Cp4.1LG02g14600 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g14600
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionVHS domain-containing protein
LocationCp4.1LG02: 13920763 .. 13924519 (+)
RNA-Seq ExpressionCp4.1LG02g14600
SyntenyCp4.1LG02g14600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGGGAAAATAAAAGAGGGAAAAATTAGACTTTTATTATTTCAGAAGGAAAAATTAGACTTTTATTATTTCAGAAGGAAAAATCTAGAAGAAGGCACAGCAAGGAATTCATCGCAATTCGCAGGTGGCATTGTAAACGTGAATTTCAGCTGTGGGCCGACATGTGGTCGTCACCTTCATGGACCGGGTGAAACCAAAATTCCTCCAATTTTCCGCCATTTTTTTCCCTCTTCAGATTGCTAGGACCTCAATTCCGATACCCAATTACGTTTCACGTCTACCCCTTGACGATCTCCGGACAAAGATCGCCTGAATTGGGAGCTGCAAAAGTTTCAATTCCAGATGAATCTGAGTTGGTTTGCGCGGTGAGACTGCTTGGAGAACAAGGAAAACATTGACTAAGCCAATGGGTTTTCTGCATTTCTTTGGTTTCTGGGATTTGGATCAGGGGTGGGATTGATTTAGGGTGGATCGGGTCTGAAGCGATTGTTTTTCAGTTGGTGATTTTCATTGGTCAATATGGATTCGAGTCGGAGAGCTGTAGAGTCGTACTGGAGGTCGCGTATGATCGATGCGGCGACTTCGGATGAGGATAAGGTCACGCCGGTGTACAAATTGGAAGAGATTTGTGAAGTGTTGAGATCTTCGCATGTCAGTATTGTCAAGGAATTTTCGGAATTTATCTTGAAGAGGCTTGAACATAAGAGCCCGATTGTCAAACAGAAGGTGTCTATAATTTTTTCTTTTCTTGTTCTGATTTCTCTCGTGTTAGTTTTCAACTTAATTGGTAGATATGTTATGAACGAGTAGCATTTTTTATCCCTGCAGTTAGGACTTTTGTTCTCTGATTCTTCTTTGGTTTTTAAATTTGATAATGTTCAAATGTTGATGGAAGAATGAACACAAGAACACAAGAACACAAGAACTTTTTCAGTTGAATGATTTCTATTATGTATGTTTCTTGTTTTTGAAAATTTTGGATTTGTGGTTTGATTTCACCACAGGCTCTCAGGTTGACTAAGTATGCAGTTGGGAAATCTGGTGTGGAATTCGGAAGGGAAATGCAGAGACACTCTGTGGCTGTCCGCCAGTTACTTCATTACAAGGGACAGCCAGACCCCCTTAAAGGTGATGCACTTAATAAAGCTGTGAGGGATACTGCTCAGGACGCCATTTCTGCGATCTTTGCTGAAGAGGACAACAGGCCTGCACCATCTGAGAATCTTAACAGTCGAATTCAAGGTTTTGGGAACTCAAATTATGAACCACCAGCAGAAGATAAAAAATCATTTCTTAGCGAGGTAGTTGGTTTAGGAAGTGCATCAATCAAGCAAGGGTTAAGTAATCTTGCACAAGGTCATTCCTCGAGAAAGAATGGCACTAGTGGCCACAGGGGTCCCAATCTTCAGAGGTCGTTGACTACTGAAATGGAGTATGACAATAGATATGAACCAGTTGAATATGGCCGTGAGACTCTCGGGACATCAAAGAGTACGATTAGTGGACCATGGAACCCGGATTCTTGGGCAAATAAGGTGGAAGCTACTAATGGGAACCTGAGTTCTGGGTCTTCGGAGAGAAAAACTCGAGAAGAGAGGTTACTGGAGACCATTGCAACAGCAGGTGGTGTGCGCATACAACCAACTCGAGATGCCATTCAAGCATTTCTTGTGGAAGCTGCAAAGTTAGATGCAATGGCGCTGAGTAGTGCTCTTGAATCAAAGCTTAAATCCCCATCATGGCAGGCATGTTCTTGCTGTTAATATTCTTTTCTATGATACTATAAGAGCAAAGCCAGGCTTTCTGTTTAGGATTGAAATAATGACGGTTGATTTTGTATCAATGTCACTCAACCTGTTTTGACGAGAATTTCATTCTTCAGGTTCGTTTCAAAGCTCTCTGCGTCCTTGAGTCGATCGTTAGGAAAAAGGATGACGATCATTTTTCGATTGTGGCATCGTATTTCAGTGAAAATCAAGATGCAGTGATTGGATGTTCTGAATCTCCCCAAGCATCTCTTAGGGAAAAAGCTAGCAAGGTAAATCATCAGTCTACCTGCAAACGATCGTTGATGAAATTGCTGAATAAATTCATTAATTAGATTTTTATGTAGCTTATTGTAAGCTTGAAAAACATGTGATGGATCAAAAAGTAATGTATTCTCAACTTCGGGTTCTTATCCCGAAATGATATAATTGAGCCCTTCGACTCTTTGGGCAGGTTATGCCACTTTTAGATGGAGGAAAAGGAGTCCCCTCCATGAATGATTCTGAAAAGTCCCGGCCAAACAACCCCAGTTCCACTATTCAGATGCCAGACTTAATAGACACAAGTGATGCAGGTGTTTTAGAGGTTGAAAACCTGTCGAAAACTCCATTAGTAGACGACTTATTTGGAGATGGCGTAAACACCGTCACAAGCACCAGCGAACTAAAGAATGATGATGACCCATTTTCAGATGTCTCTTTTCAGACAACTGATAATGGAGAAAATCCAGATGATCTTTTTTCTGGGATGAACGTCGATAATGGTCAGGTTAGTAATGAAAATAAAAGGCCTGCCTCGGAACAGAAAAATGAACATGGAGTTTTTTATGTTTTTGGATCAAGTTCTGAAGCTGCAGTACAAGAACACACAAGGAAAGATGTTAATGACTTAATGAGTGGTTTGTCCATCCATGAAAATGCCTTGAAGAGTAATGATATAGGAGATTCCAAGGATTCACTGTCTGAATCTTTATATTCTGTTTCCAGTCAGCCAAACCATCAGTATCAGACTAAAGATTCTTCTGTAAATGGCATATACAGTTCACCAATGGTTGGGACAAATATGAATGCTGCCTTCCTCCCTGGAATGACATATCTTCCGTCCGGCATGATGTTCAATCCAGCCTTTTCATCTCAGCCAATGGGTTATGCTCCCACAGGAAACTTCTTTGCTCAACAAAATCTAGTATCAGCCATGTCCAATTACCAACAGTTTGGGAACCCTCATCTCCAATCAAGTGGTGGAAGTGCGGGTAATGGAGGATATTCTTCACCCCTTCCTGACATATTCCAGCCAAATCTTGCAACACAGCCATCTAGTTCCGTGATGAATAGTTCAAAGAAAGAAGATACCAGAGCTTTTGATTTTATCTCAGTAAGTTTCATTTCTTACAACTCTTTGTTGATTTTAGTAGCTGTATGCCCTGCAGTCTGATACTGATAGAGCATTACGATGTATTTTGTAGGAGCACATTGCAGCTGCTCGGGATCCAAAGCGGGTAGTCTGAGTTTAGGATTGTTGGTGACGTCGGTGACATTGGGGAGGTGATGAATGAAAGCCACAGTTCTGTGCCATTAGAGATTGATGGCTCCATAACGTAGGCACTATCGACGAACCACAACACGGTACACACTTATGAAAAATGGGTAGATAGAAGTAGGAAAAAGAGAAAATGGAAGTTGAAGAAGAATGAGAGTTGCTTTTACATAATCAGCTGTTTATACTGTGGTAATTCCTGGACTGGTTGTGTTCCAATCTGTAGAGTTTCATTTTGCCCAAAAAAGGAAAGAAAAAAGGAAAAAAAAAAAACTTATTATTGTAGTGGTTATGGTAATAATGCAGCTTGTTCTTTGAATCTTTTAATGCAGCTTGTTCTTTCATGTATATGCCTGTGTATGTTGAGTTCTTAGATTGACACTCATAAGCTGAGTTATTAATATAACACTTCATTCTTGAATGGTACAATCAAAGGAAACTATTGATATTATTG

mRNA sequence

AGAGGGAAAATAAAAGAGGGAAAAATTAGACTTTTATTATTTCAGAAGGAAAAATTAGACTTTTATTATTTCAGAAGGAAAAATCTAGAAGAAGGCACAGCAAGGAATTCATCGCAATTCGCAGGTGGCATTGTAAACGTGAATTTCAGCTGTGGGCCGACATGTGGTCGTCACCTTCATGGACCGGGTGAAACCAAAATTCCTCCAATTTTCCGCCATTTTTTTCCCTCTTCAGATTGCTAGGACCTCAATTCCGATACCCAATTACGTTTCACGTCTACCCCTTGACGATCTCCGGACAAAGATCGCCTGAATTGGGAGCTGCAAAAGTTTCAATTCCAGATGAATCTGAGTTGGTTTGCGCGGTGAGACTGCTTGGAGAACAAGGAAAACATTGACTAAGCCAATGGGTTTTCTGCATTTCTTTGGTTTCTGGGATTTGGATCAGGGGTGGGATTGATTTAGGGTGGATCGGGTCTGAAGCGATTGTTTTTCAGTTGGTGATTTTCATTGGTCAATATGGATTCGAGTCGGAGAGCTGTAGAGTCGTACTGGAGGTCGCGTATGATCGATGCGGCGACTTCGGATGAGGATAAGGTCACGCCGGTGTACAAATTGGAAGAGATTTGTGAAGTGTTGAGATCTTCGCATGTCAGTATTGTCAAGGAATTTTCGGAATTTATCTTGAAGAGGCTTGAACATAAGAGCCCGATTGTCAAACAGAAGGCTCTCAGGTTGACTAAGTATGCAGTTGGGAAATCTGGTGTGGAATTCGGAAGGGAAATGCAGAGACACTCTGTGGCTGTCCGCCAGTTACTTCATTACAAGGGACAGCCAGACCCCCTTAAAGGTGATGCACTTAATAAAGCTGTGAGGGATACTGCTCAGGACGCCATTTCTGCGATCTTTGCTGAAGAGGACAACAGGCCTGCACCATCTGAGAATCTTAACAGTCGAATTCAAGGTTTTGGGAACTCAAATTATGAACCACCAGCAGAAGATAAAAAATCATTTCTTAGCGAGGTAGTTGGTTTAGGAAGTGCATCAATCAAGCAAGGGTTAAGTAATCTTGCACAAGGTCATTCCTCGAGAAAGAATGGCACTAGTGGCCACAGGGGTCCCAATCTTCAGAGGTCGTTGACTACTGAAATGGAGTATGACAATAGATATGAACCAGTTGAATATGGCCGTGAGACTCTCGGGACATCAAAGAGTACGATTAGTGGACCATGGAACCCGGATTCTTGGGCAAATAAGGTGGAAGCTACTAATGGGAACCTGAGTTCTGGGTCTTCGGAGAGAAAAACTCGAGAAGAGAGGTTACTGGAGACCATTGCAACAGCAGGTGGTGTGCGCATACAACCAACTCGAGATGCCATTCAAGCATTTCTTGTGGAAGCTGCAAAGTTAGATGCAATGGCGCTGAGTAGTGCTCTTGAATCAAAGCTTAAATCCCCATCATGGCAGGTTCGTTTCAAAGCTCTCTGCGTCCTTGAGTCGATCGTTAGGAAAAAGGATGACGATCATTTTTCGATTGTGGCATCGTATTTCAGTGAAAATCAAGATGCAGTGATTGGATGTTCTGAATCTCCCCAAGCATCTCTTAGGGAAAAAGCTAGCAAGGTTATGCCACTTTTAGATGGAGGAAAAGGAGTCCCCTCCATGAATGATTCTGAAAAGTCCCGGCCAAACAACCCCAGTTCCACTATTCAGATGCCAGACTTAATAGACACAAGTGATGCAGGTGTTTTAGAGGTTGAAAACCTGTCGAAAACTCCATTAGTAGACGACTTATTTGGAGATGGCGTAAACACCGTCACAAGCACCAGCGAACTAAAGAATGATGATGACCCATTTTCAGATGTCTCTTTTCAGACAACTGATAATGGAGAAAATCCAGATGATCTTTTTTCTGGGATGAACGTCGATAATGGTCAGGTTAGTAATGAAAATAAAAGGCCTGCCTCGGAACAGAAAAATGAACATGGAGTTTTTTATGTTTTTGGATCAAGTTCTGAAGCTGCAGTACAAGAACACACAAGGAAAGATGTTAATGACTTAATGAGTGGTTTGTCCATCCATGAAAATGCCTTGAAGAGTAATGATATAGGAGATTCCAAGGATTCACTGTCTGAATCTTTATATTCTGTTTCCAGTCAGCCAAACCATCAGTATCAGACTAAAGATTCTTCTGTAAATGGCATATACAGTTCACCAATGGTTGGGACAAATATGAATGCTGCCTTCCTCCCTGGAATGACATATCTTCCGTCCGGCATGATGTTCAATCCAGCCTTTTCATCTCAGCCAATGGGTTATGCTCCCACAGGAAACTTCTTTGCTCAACAAAATCTAGTATCAGCCATGTCCAATTACCAACAGTTTGGGAACCCTCATCTCCAATCAAGTGGTGGAAGTGCGGGTAATGGAGGATATTCTTCACCCCTTCCTGACATATTCCAGCCAAATCTTGCAACACAGCCATCTAGTTCCGTGATGAATAGTTCAAAGAAAGAAGATACCAGAGCTTTTGATTTTATCTCAGAGCACATTGCAGCTGCTCGGGATCCAAAGCGGGTAGTCTGAGTTTAGGATTGTTGGTGACGTCGGTGACATTGGGGAGGTGATGAATGAAAGCCACAGTTCTGTGCCATTAGAGATTGATGGCTCCATAACGTAGGCACTATCGACGAACCACAACACGGTACACACTTATGAAAAATGGGTAGATAGAAGTAGGAAAAAGAGAAAATGGAAGTTGAAGAAGAATGAGAGTTGCTTTTACATAATCAGCTGTTTATACTGTGGTAATTCCTGGACTGGTTGTGTTCCAATCTGTAGAGTTTCATTTTGCCCAAAAAAGGAAAGAAAAAAGGAAAAAAAAAAAACTTATTATTGTAGTGGTTATGGTAATAATGCAGCTTGTTCTTTGAATCTTTTAATGCAGCTTGTTCTTTCATGTATATGCCTGTGTATGTTGAGTTCTTAGATTGACACTCATAAGCTGAGTTATTAATATAACACTTCATTCTTGAATGGTACAATCAAAGGAAACTATTGATATTATTG

Coding sequence (CDS)

ATGGATTCGAGTCGGAGAGCTGTAGAGTCGTACTGGAGGTCGCGTATGATCGATGCGGCGACTTCGGATGAGGATAAGGTCACGCCGGTGTACAAATTGGAAGAGATTTGTGAAGTGTTGAGATCTTCGCATGTCAGTATTGTCAAGGAATTTTCGGAATTTATCTTGAAGAGGCTTGAACATAAGAGCCCGATTGTCAAACAGAAGGCTCTCAGGTTGACTAAGTATGCAGTTGGGAAATCTGGTGTGGAATTCGGAAGGGAAATGCAGAGACACTCTGTGGCTGTCCGCCAGTTACTTCATTACAAGGGACAGCCAGACCCCCTTAAAGGTGATGCACTTAATAAAGCTGTGAGGGATACTGCTCAGGACGCCATTTCTGCGATCTTTGCTGAAGAGGACAACAGGCCTGCACCATCTGAGAATCTTAACAGTCGAATTCAAGGTTTTGGGAACTCAAATTATGAACCACCAGCAGAAGATAAAAAATCATTTCTTAGCGAGGTAGTTGGTTTAGGAAGTGCATCAATCAAGCAAGGGTTAAGTAATCTTGCACAAGGTCATTCCTCGAGAAAGAATGGCACTAGTGGCCACAGGGGTCCCAATCTTCAGAGGTCGTTGACTACTGAAATGGAGTATGACAATAGATATGAACCAGTTGAATATGGCCGTGAGACTCTCGGGACATCAAAGAGTACGATTAGTGGACCATGGAACCCGGATTCTTGGGCAAATAAGGTGGAAGCTACTAATGGGAACCTGAGTTCTGGGTCTTCGGAGAGAAAAACTCGAGAAGAGAGGTTACTGGAGACCATTGCAACAGCAGGTGGTGTGCGCATACAACCAACTCGAGATGCCATTCAAGCATTTCTTGTGGAAGCTGCAAAGTTAGATGCAATGGCGCTGAGTAGTGCTCTTGAATCAAAGCTTAAATCCCCATCATGGCAGGTTCGTTTCAAAGCTCTCTGCGTCCTTGAGTCGATCGTTAGGAAAAAGGATGACGATCATTTTTCGATTGTGGCATCGTATTTCAGTGAAAATCAAGATGCAGTGATTGGATGTTCTGAATCTCCCCAAGCATCTCTTAGGGAAAAAGCTAGCAAGGTTATGCCACTTTTAGATGGAGGAAAAGGAGTCCCCTCCATGAATGATTCTGAAAAGTCCCGGCCAAACAACCCCAGTTCCACTATTCAGATGCCAGACTTAATAGACACAAGTGATGCAGGTGTTTTAGAGGTTGAAAACCTGTCGAAAACTCCATTAGTAGACGACTTATTTGGAGATGGCGTAAACACCGTCACAAGCACCAGCGAACTAAAGAATGATGATGACCCATTTTCAGATGTCTCTTTTCAGACAACTGATAATGGAGAAAATCCAGATGATCTTTTTTCTGGGATGAACGTCGATAATGGTCAGGTTAGTAATGAAAATAAAAGGCCTGCCTCGGAACAGAAAAATGAACATGGAGTTTTTTATGTTTTTGGATCAAGTTCTGAAGCTGCAGTACAAGAACACACAAGGAAAGATGTTAATGACTTAATGAGTGGTTTGTCCATCCATGAAAATGCCTTGAAGAGTAATGATATAGGAGATTCCAAGGATTCACTGTCTGAATCTTTATATTCTGTTTCCAGTCAGCCAAACCATCAGTATCAGACTAAAGATTCTTCTGTAAATGGCATATACAGTTCACCAATGGTTGGGACAAATATGAATGCTGCCTTCCTCCCTGGAATGACATATCTTCCGTCCGGCATGATGTTCAATCCAGCCTTTTCATCTCAGCCAATGGGTTATGCTCCCACAGGAAACTTCTTTGCTCAACAAAATCTAGTATCAGCCATGTCCAATTACCAACAGTTTGGGAACCCTCATCTCCAATCAAGTGGTGGAAGTGCGGGTAATGGAGGATATTCTTCACCCCTTCCTGACATATTCCAGCCAAATCTTGCAACACAGCCATCTAGTTCCGTGATGAATAGTTCAAAGAAAGAAGATACCAGAGCTTTTGATTTTATCTCAGAGCACATTGCAGCTGCTCGGGATCCAAAGCGGGTAGTCTGA

Protein sequence

MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLEHKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQGLSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNPDSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQASLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTPLVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKRPASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSESLYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGYAPTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFISEHIAAARDPKRVV
Homology
BLAST of Cp4.1LG02g14600 vs. ExPASy Swiss-Prot
Match: Q9C5H4 (Protein MODIFIED TRANSPORT TO THE VACUOLE 1 OS=Arabidopsis thaliana OX=3702 GN=MTV1 PE=1 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 2.1e-190
Identity = 398/708 (56.21%), Postives = 498/708 (70.34%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MD+SRRAVESYWRSRMIDA TSDEDKV PVYKLEEIC++LRSSHVSIVKEFSEFILKRL+
Sbjct: 1   MDTSRRAVESYWRSRMIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLD 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           +KSPIVKQKALRL KYAVGKSG EF REMQR+SVAVR L HYKG PDPLKGDALNKAVR+
Sbjct: 61  NKSPIVKQKALRLIKYAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRE 120

Query: 121 TAQDAISAIFAEED-NRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQ 180
           TA + ISAIF+EE+  +PA  E++N RI+GFGN+N++ P+ D KSFLSEVVG+GSASIKQ
Sbjct: 121 TAHETISAIFSEENGTKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQ 180

Query: 181 GLSNLAQGHSSRK--NGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRE-TLGTSKSTISG 240
           G+SN AQGH  +K  NG+S +RGPNL RSLT E E  +RY+PV+ G++   GTSK+T  G
Sbjct: 181 GISNFAQGHLPKKNENGSSSYRGPNLHRSLTMENENFSRYDPVKLGKDGNYGTSKNTTGG 240

Query: 241 PWNPDSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAK 300
                SW +     + + +S   E KTREE+LLETI T+GGVR+QPTRDA+  F++EAAK
Sbjct: 241 -----SWGHASGEASESSASVRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEAAK 300

Query: 301 LDAMALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSE 360
           +DA+ALS AL+ KL SP WQVR KALCVLE+I+RKK+D++FSIV +YFSEN DA+  C+E
Sbjct: 301 MDAVALSIALDGKLHSPMWQVRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRCAE 360

Query: 361 SPQASLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDA-------G 420
           SPQ+SLREKA+KV+ LL+GG+    M+ S+ +      + + +PDLIDT D+        
Sbjct: 361 SPQSSLREKANKVLSLLNGGQSSGLMSSSDNTVKR--EAAVDLPDLIDTGDSDDTLNNLN 420

Query: 421 VLEVENLSKT--PLV-DDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSG 480
            ++  +   T  PL+ DD FGD  +   S+SE K DDDPF+DVSF   +  E+ DDLFSG
Sbjct: 421 AIDTGSTVATAGPLMDDDWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKESADDLFSG 480

Query: 481 MNVDNGQVSNENKRPASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALK 540
           M V         K  A    +   +F +FGS+++   +    K++NDLM   SI EN   
Sbjct: 481 MTVG-------EKSAAVGGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFSIDEN--N 540

Query: 541 SNDIGDSKDSLSESLYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPG--MTY-L 600
           SN  G S  +L + L+++ S  +H  Q  ++ V GI  S   G   N   LPG  M +  
Sbjct: 541 SNQKGSSSSTLPQDLFAMPSTTSH--QAPENPVGGILGSQNPGFIQN-TMLPGGVMPFNF 600

Query: 601 PSGMMFNPAFSSQPMGYAPTGNFFA-QQNLVSAMSNYQQFGNPHLQSSGG--SAG-NGGY 660
           P GMM NPAF+SQP+ YA   +  A QQ  +  MSN+QQFGN + Q SG   S G +GG 
Sbjct: 601 PQGMMMNPAFASQPLNYAAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLSMGTSGGN 660

Query: 661 SSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFISEHIAAARDPKRV 688
            S LPDIFQPN   Q  +S MN SKKEDTRAFDFIS+H+ +ARD KRV
Sbjct: 661 QSALPDIFQPNFGNQAPTSTMNGSKKEDTRAFDFISDHLTSARDTKRV 689

BLAST of Cp4.1LG02g14600 vs. ExPASy Swiss-Prot
Match: G3V8Y7 (AP-4 complex accessory subunit Tepsin OS=Rattus norvegicus OX=10116 GN=Tepsin PE=1 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 5.2e-08
Identity = 47/147 (31.97%), Postives = 73/147 (49.66%), Query Frame = 0

Query: 21  TSDEDKVTPVYKLEEICEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLTKYAV 80
           TSD+D   P Y  EEI ++   SH S+       E++L RL+  S  VK K L++  Y  
Sbjct: 24  TSDDDIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 81  GKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDNRPA 140
                 F   ++R+S  +++   + G PDPL G++L + VR  AQD  S +F++   +P 
Sbjct: 84  SHGSSSFMLILRRNSALIQEATAFAGPPDPLHGNSLYQKVRAAAQDLGSTLFSDALPQP- 143

Query: 141 PSE--------------NLNSRIQGFG 152
           PS+                +S +QGFG
Sbjct: 144 PSQPPQILPPAGMGAQARPHSALQGFG 166

BLAST of Cp4.1LG02g14600 vs. ExPASy Swiss-Prot
Match: Q3U3N6 (AP-4 complex accessory subunit Tepsin OS=Mus musculus OX=10090 GN=Tepsin PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 6.3e-06
Identity = 89/359 (24.79%), Postives = 146/359 (40.67%), Query Frame = 0

Query: 21  TSDEDKVTPVYKLEEICEVLRSSHVSIVKE--FSEFILKRLEHKSPIVKQKALRLTKYAV 80
           TSD+    P Y  EEI ++   SH S+       E++L RL+  S  VK K L++  Y  
Sbjct: 24  TSDDSIPCPGYLFEEIAKI---SHESLGSSQCLLEYLLNRLDSSSGHVKLKVLKILLYLC 83

Query: 81  GKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRDTAQDAISAIFAEEDNRP- 140
           G     F   ++R+S  +++   + G PDPL G++L + VR  AQD  S +F++   +P 
Sbjct: 84  GHGSSSFLLILRRNSALIQEATAFSGPPDPLHGNSLYQKVRAAAQDLGSTLFSDAVPQPP 143

Query: 141 ------APSENLN------SRIQGFG----NSNYEPPAEDKKSFLSEVVGLGSASIKQGL 200
                  P   +       S +QGFG    +S      E   S +     + + +++ G 
Sbjct: 144 SQPPQIPPPAGMGAQARPLSALQGFGYTKESSRTGSAGETFLSTIQRAAEVVANAVRPGP 203

Query: 201 SN------LAQGHSSRKNGT--SGHRGPNLQRSLTTEM--EYDNRYEPVEYGR--ETLGT 260
            N      L  G S +   T  + H  PN    L   +      R++P + G   + L +
Sbjct: 204 DNPCTKGPLPYGDSYQPAVTPSASHTHPNPGNLLPGAILGARAVRHQPGQAGGGWDELDS 263

Query: 261 SKSTISGPWNPDSWANKVEATNGNLSSGSSERK---------------------TREERL 320
           S S+     N    +N   A++    SGS                          +E  L
Sbjct: 264 SPSS----QNSSCTSNLSRASDSGSRSGSDSHSGTSREPGDLAERAEATPPNDCQQELNL 323

Query: 321 LETIATAGGVRIQPTRDAIQAFLVEAAKLDAMALSSALESKLKSPSWQVRFKALCVLES 328
           + T+    G R+  +R+  Q F+ E   L+  A+   L  +L   S   + +ALC + S
Sbjct: 324 VRTVTQ--GPRVFLSREETQHFIKECGLLNCEAVLELLLRQLVGTSECEQMRALCAIAS 373

BLAST of Cp4.1LG02g14600 vs. NCBI nr
Match: XP_023524865.1 (VHS domain-containing protein At3g16270-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1331 bits (3444), Expect = 0.0
Identity = 688/688 (100.00%), Postives = 688/688 (100.00%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP
Sbjct: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM
Sbjct: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA
Sbjct: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420
           SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420

Query: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480
           LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR
Sbjct: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480

Query: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540
           PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES
Sbjct: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540

Query: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600
           LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY
Sbjct: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600

Query: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660
           APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM
Sbjct: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660

Query: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688
           NSSKKEDTRAFDFISEHIAAARDPKRVV
Sbjct: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688

BLAST of Cp4.1LG02g14600 vs. NCBI nr
Match: KAG6606757.1 (Protein MODIFIED TRANSPORT TO THE VACUOLE 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1292 bits (3343), Expect = 0.0
Identity = 669/688 (97.24%), Postives = 673/688 (97.82%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIV+QKALRL KY VGKSGVEF REMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPIVQQKALRLIKYGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTI+GPWN 
Sbjct: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTITGPWNQ 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DSWANKVEATNGNLSSGSSER+TREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM
Sbjct: 241 DSWANKVEATNGNLSSGSSERRTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALSSALESKLKSPSWQVRFKALCVLESIVRK DDDHFSIVASYFSENQDAVIGCSESPQA
Sbjct: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420
           SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG LE ENLSKTP
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGALEAENLSKTP 420

Query: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480
           LVDDLFGDGVNTV STSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDN QVSNENKR
Sbjct: 421 LVDDLFGDGVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKR 480

Query: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540
           PASEQKNEHGVF VFGSSSEA VQEHTRKDVNDLMSGLSIHE+ LKSNDIGDSKDSLSES
Sbjct: 481 PASEQKNEHGVFDVFGSSSEAGVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSES 540

Query: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600
           LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAF PGMTYLPSGMMFNPAFSSQP GY
Sbjct: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSQPRGY 600

Query: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660
           APTGNFFAQQNLVSAMSNYQQFGNP LQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM
Sbjct: 601 APTGNFFAQQNLVSAMSNYQQFGNPRLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660

Query: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688
           NSSKKEDTRAFDFISEHIAAARDPKRVV
Sbjct: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688

BLAST of Cp4.1LG02g14600 vs. NCBI nr
Match: XP_022949030.1 (VHS domain-containing protein At3g16270-like [Cucurbita moschata])

HSP 1 Score: 1291 bits (3342), Expect = 0.0
Identity = 669/688 (97.24%), Postives = 673/688 (97.82%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIVKQKALRL KY +GKSGVEF REMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLIKYGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKS ISGPWNP
Sbjct: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNP 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM
Sbjct: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALSSALESKLKSPSWQVRFKALC+LESIVRK DDDHFSIVASYFSENQDAVIGCSESPQA
Sbjct: 301 ALSSALESKLKSPSWQVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420
           SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLE ENLSKTP
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTP 420

Query: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480
           LVD LFGDGVNTV STSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDN QVSNENKR
Sbjct: 421 LVDVLFGDGVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKR 480

Query: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540
           PASEQKNEHGVF V GSSSEAAVQEHTRKDVNDLMSGLSIHE+ LKSNDIGDSKDSLSES
Sbjct: 481 PASEQKNEHGVFDVLGSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSES 540

Query: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600
           LYSVS QPNHQYQTKDSSVNGIYSSPMVGTNMNAAF PGMTYLPSGMMFNPAFSS+PMGY
Sbjct: 541 LYSVSGQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGY 600

Query: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660
           APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM
Sbjct: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660

Query: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688
           NSSKKEDTRAFDFISEHIAAARDPKRVV
Sbjct: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688

BLAST of Cp4.1LG02g14600 vs. NCBI nr
Match: XP_022997847.1 (VHS domain-containing protein At3g16270-like [Cucurbita maxima])

HSP 1 Score: 1282 bits (3317), Expect = 0.0
Identity = 666/688 (96.80%), Postives = 672/688 (97.67%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIVKQKALRL KY VGKSGVEF REMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLIKYGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP
Sbjct: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM
Sbjct: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALSSALESKLKSPSWQVRFKALCVLESIVRK DDDHFSIVASYFSENQDAVIGCSESPQA
Sbjct: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420
           SLREKASKVMPLLDGGKGVPSMN SEKS PNNPSSTIQMPDLIDTSDAGVLEVENLSKTP
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNASEKSLPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420

Query: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480
           LVDDLFGDGVNT+TSTSELKNDDDPFSDVSFQTTD+GENPDDLFSGM VDN QVSNE+KR
Sbjct: 421 LVDDLFGDGVNTITSTSELKNDDDPFSDVSFQTTDDGENPDDLFSGMIVDNSQVSNESKR 480

Query: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540
           PASEQKNEHGVF VFGSSSEAAVQEHTRKDVN+LMSGLSIHE+ LKSNDIGDSKDSLSES
Sbjct: 481 PASEQKNEHGVFDVFGSSSEAAVQEHTRKDVNELMSGLSIHEDGLKSNDIGDSKDSLSES 540

Query: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600
           LYSVS QPNHQYQTKDSSVNGIYSSPMVGTNMNAA  PG TYLPSGMMFNPAFSSQPMGY
Sbjct: 541 LYSVSRQPNHQYQTKDSSVNGIYSSPMVGTNMNAALFPGRTYLPSGMMFNPAFSSQPMGY 600

Query: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660
           APTGNFFAQQNL SAMSN QQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM
Sbjct: 601 APTGNFFAQQNLPSAMSNSQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660

Query: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688
           NSSKKEDTRAFDFISEHIAAARDPK+VV
Sbjct: 661 NSSKKEDTRAFDFISEHIAAARDPKQVV 688

BLAST of Cp4.1LG02g14600 vs. NCBI nr
Match: KAG7036470.1 (VHS domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1173 bits (3034), Expect = 0.0
Identity = 619/688 (89.97%), Postives = 624/688 (90.70%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVT VYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTLVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIV+QKALRL KY VGKSGVEF REMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPIVQQKALRLIKYGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTI+GPWN 
Sbjct: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTITGPWNQ 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DSWANKVEATNGNLSSGSSER+TREERLLETIATAGGVRIQPTRDAIQAFLVEAA     
Sbjct: 241 DSWANKVEATNGNLSSGSSERRTREERLLETIATAGGVRIQPTRDAIQAFLVEAA----- 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
                                                       +ENQDAVIGCSESPQA
Sbjct: 301 --------------------------------------------NENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420
           SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG LE ENLSKTP
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGALEAENLSKTP 420

Query: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480
           LVDDLFGDGVNTV STSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDN QVSNENKR
Sbjct: 421 LVDDLFGDGVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKR 480

Query: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540
           PASEQKNEHGVF VFGSSSEA VQEHTRKDVNDLMSGLSIHE+ LKSNDIGDSKDSLSES
Sbjct: 481 PASEQKNEHGVFDVFGSSSEAGVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSES 540

Query: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600
           LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAF PGMTYLPSGMMFNPAFSSQP GY
Sbjct: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSQPRGY 600

Query: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660
           APTGNFFAQQNLVSAMSNYQQFGNP LQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM
Sbjct: 601 APTGNFFAQQNLVSAMSNYQQFGNPRLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 639

Query: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688
           NSSKKEDTRAFDFISEHIAAARDPKRVV
Sbjct: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 639

BLAST of Cp4.1LG02g14600 vs. ExPASy TrEMBL
Match: A0A6J1GBM7 (VHS domain-containing protein At3g16270-like OS=Cucurbita moschata OX=3662 GN=LOC111452495 PE=4 SV=1)

HSP 1 Score: 1291 bits (3342), Expect = 0.0
Identity = 669/688 (97.24%), Postives = 673/688 (97.82%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEI EVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEISEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIVKQKALRL KY +GKSGVEF REMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLIKYGIGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKS ISGPWNP
Sbjct: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSMISGPWNP 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM
Sbjct: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALSSALESKLKSPSWQVRFKALC+LESIVRK DDDHFSIVASYFSENQDAVIGCSESPQA
Sbjct: 301 ALSSALESKLKSPSWQVRFKALCILESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420
           SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLE ENLSKTP
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEAENLSKTP 420

Query: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480
           LVD LFGDGVNTV STSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDN QVSNENKR
Sbjct: 421 LVDVLFGDGVNTVASTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNSQVSNENKR 480

Query: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540
           PASEQKNEHGVF V GSSSEAAVQEHTRKDVNDLMSGLSIHE+ LKSNDIGDSKDSLSES
Sbjct: 481 PASEQKNEHGVFDVLGSSSEAAVQEHTRKDVNDLMSGLSIHEDGLKSNDIGDSKDSLSES 540

Query: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600
           LYSVS QPNHQYQTKDSSVNGIYSSPMVGTNMNAAF PGMTYLPSGMMFNPAFSS+PMGY
Sbjct: 541 LYSVSGQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFFPGMTYLPSGMMFNPAFSSRPMGY 600

Query: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660
           APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM
Sbjct: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660

Query: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688
           NSSKKEDTRAFDFISEHIAAARDPKRVV
Sbjct: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688

BLAST of Cp4.1LG02g14600 vs. ExPASy TrEMBL
Match: A0A6J1K668 (VHS domain-containing protein At3g16270-like OS=Cucurbita maxima OX=3661 GN=LOC111492683 PE=4 SV=1)

HSP 1 Score: 1282 bits (3317), Expect = 0.0
Identity = 666/688 (96.80%), Postives = 672/688 (97.67%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIVKQKALRL KY VGKSGVEF REMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPIVKQKALRLIKYGVGKSGVEFRREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP
Sbjct: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM
Sbjct: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALSSALESKLKSPSWQVRFKALCVLESIVRK DDDHFSIVASYFSENQDAVIGCSESPQA
Sbjct: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKNDDDHFSIVASYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420
           SLREKASKVMPLLDGGKGVPSMN SEKS PNNPSSTIQMPDLIDTSDAGVLEVENLSKTP
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNASEKSLPNNPSSTIQMPDLIDTSDAGVLEVENLSKTP 420

Query: 421 LVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQVSNENKR 480
           LVDDLFGDGVNT+TSTSELKNDDDPFSDVSFQTTD+GENPDDLFSGM VDN QVSNE+KR
Sbjct: 421 LVDDLFGDGVNTITSTSELKNDDDPFSDVSFQTTDDGENPDDLFSGMIVDNSQVSNESKR 480

Query: 481 PASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDSKDSLSES 540
           PASEQKNEHGVF VFGSSSEAAVQEHTRKDVN+LMSGLSIHE+ LKSNDIGDSKDSLSES
Sbjct: 481 PASEQKNEHGVFDVFGSSSEAAVQEHTRKDVNELMSGLSIHEDGLKSNDIGDSKDSLSES 540

Query: 541 LYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAFSSQPMGY 600
           LYSVS QPNHQYQTKDSSVNGIYSSPMVGTNMNAA  PG TYLPSGMMFNPAFSSQPMGY
Sbjct: 541 LYSVSRQPNHQYQTKDSSVNGIYSSPMVGTNMNAALFPGRTYLPSGMMFNPAFSSQPMGY 600

Query: 601 APTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660
           APTGNFFAQQNL SAMSN QQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM
Sbjct: 601 APTGNFFAQQNLPSAMSNSQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLATQPSSSVM 660

Query: 661 NSSKKEDTRAFDFISEHIAAARDPKRVV 688
           NSSKKEDTRAFDFISEHIAAARDPK+VV
Sbjct: 661 NSSKKEDTRAFDFISEHIAAARDPKQVV 688

BLAST of Cp4.1LG02g14600 vs. ExPASy TrEMBL
Match: A0A1S3BH26 (VHS domain-containing protein At3g16270 OS=Cucumis melo OX=3656 GN=LOC103489958 PE=4 SV=1)

HSP 1 Score: 1130 bits (2923), Expect = 0.0
Identity = 592/696 (85.06%), Postives = 628/696 (90.23%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSP+VKQKALRL KYAVGKSGVEF REMQR+SVAVRQL HYKGQPDPLKGDALNKAVRD
Sbjct: 61  HKSPVVKQKALRLIKYAVGKSGVEFRREMQRNSVAVRQLFHYKGQPDPLKGDALNKAVRD 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TA +AIS+IFAEEDN+PAPSENLN RIQGFGNSNYEPP+EDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAHEAISSIFAEEDNKPAPSENLNRRIQGFGNSNYEPPSEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSN AQGHSSRKNGTS HRG NLQRSLTTEMEYDNRYEPVEYGRETLGT++ST SG WN 
Sbjct: 181 LSNFAQGHSSRKNGTSSHRGINLQRSLTTEMEYDNRYEPVEYGRETLGTARSTTSGTWNQ 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DS       +NG+ SSGSSE KTRE+RLL+TIATAGGVR+QPTRD+IQAFLVEA KLDA+
Sbjct: 241 DS-----RVSNGSPSSGSSESKTREDRLLDTIATAGGVRLQPTRDSIQAFLVEAVKLDAL 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALS+ALE+KLKSPSWQVRFKALC+LESIVR+ DDDHFSIV SYFSENQ+AVIGCSESPQA
Sbjct: 301 ALSNALETKLKSPSWQVRFKALCILESIVRRNDDDHFSIVTSYFSENQEAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG-------VLEV 420
           SLREKASKVMPLLDGGKGVPSMN SEKS P+N SSTIQMPDLIDTSDAG        +EV
Sbjct: 361 SLREKASKVMPLLDGGKGVPSMNVSEKSLPSNTSSTIQMPDLIDTSDAGDYSGTNKSVEV 420

Query: 421 ENLSKTPLVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQ 480
           ENLS TPLVDDLFGDG+NTVTSTSELKNDDDPFSDVSF T +  ENPDDLFSGMN DN Q
Sbjct: 421 ENLSSTPLVDDLFGDGLNTVTSTSELKNDDDPFSDVSFHTIETRENPDDLFSGMNFDNNQ 480

Query: 481 VSNENKRPASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDS 540
           VSNENK+PA EQKNE GVF +FGSSSE AVQEH RKDVNDLMSGLSIHE+ LKS D GDS
Sbjct: 481 VSNENKKPALEQKNEPGVFDIFGSSSEPAVQEHARKDVNDLMSGLSIHEDTLKSKDKGDS 540

Query: 541 KDSLSESLYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAF 600
           KDSLSESL+S S QPNHQ      S+NGIYSSPM GTNMNAAF PGMTYLPSGMMFNPAF
Sbjct: 541 KDSLSESLFSASGQPNHQNPVSQDSLNGIYSSPMAGTNMNAAFFPGMTYLPSGMMFNPAF 600

Query: 601 SSQPMGYAPTGNFFAQQNLVSAMSNYQQFGNPHLQS-SGGSAGNGGYSSPLPDIFQPNLA 660
           SSQPM YA +GNFF QQ L+SAMSNYQQFGNP+LQS SGG  G+GGYSSPLPDIFQPNLA
Sbjct: 601 SSQPMAYAASGNFFTQQQLLSAMSNYQQFGNPNLQSNSGGGVGSGGYSSPLPDIFQPNLA 660

Query: 661 TQPSSSVMNSSKKEDTRAFDFISEHIAAARDPKRVV 688
            Q S+SVMNSSKKEDTRAFDFIS+H+AAARDPKRVV
Sbjct: 661 AQSSTSVMNSSKKEDTRAFDFISDHVAAARDPKRVV 691

BLAST of Cp4.1LG02g14600 vs. ExPASy TrEMBL
Match: A0A6J1IW71 (VHS domain-containing protein At3g16270-like OS=Cucurbita maxima OX=3661 GN=LOC111479156 PE=4 SV=1)

HSP 1 Score: 1123 bits (2905), Expect = 0.0
Identity = 592/695 (85.18%), Postives = 622/695 (89.50%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIVKQKALRL KYAVGKSGVEF REMQRHSVAVRQL HYKGQPDPLKGDALNKAVR+
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRE 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TA DAISAIFAEEDN+PAPSENLN RIQGFGNSNYEPP EDKKSFLSEVVGLGSASIKQG
Sbjct: 121 TAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKQG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSN AQGHSSRKNGTS  RGPNLQRSLTTE+EYDNRYEPVEYGRETLGTSKSTISG WN 
Sbjct: 181 LSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTISGTWNQ 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DS       +NGN SSGSS  KTREERLLETIATAGGVR+QPTRDAIQAFLVEAA LDA+
Sbjct: 241 DS-----RVSNGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAMLDAL 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
           ALS+ALE+KLKSPSWQVRFKALC+LESIVR+  D+HFSIV SYFSENQDAVIGCSESPQA
Sbjct: 301 ALSNALETKLKSPSWQVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG-------VLEV 420
           SLR+KASKVMPLLDGGKGVP MNDSEKS P+N SSTIQMPDL+DTSDAG        LEV
Sbjct: 361 SLRDKASKVMPLLDGGKGVPPMNDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKSLEV 420

Query: 421 ENLSKTPLVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQ 480
           ENLS  PLVDDLFG G+NTVTSTSELKNDDDPFSDV F TT+  ENPDD+FSGMN +N Q
Sbjct: 421 ENLSSVPLVDDLFGGGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDIFSGMNFENNQ 480

Query: 481 VSNENKRPASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDS 540
           V++ENK+P SEQKNE GVF +FGSSSE AVQEH RKDV DLMSGLSIHE+ALK+ D GDS
Sbjct: 481 VTDENKKPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDS 540

Query: 541 KDSLSESLYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAF 600
           KDSLSESL+SVSSQPNHQ Q    S+ G YSSPMVGTNMNA F PGM YLPSGMMFNPAF
Sbjct: 541 KDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATFFPGMPYLPSGMMFNPAF 600

Query: 601 SSQPMGYAPTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLAT 660
           SSQPMGYAPTGNFF QQ L+SAMSNYQQFGNP+LQSSGG     GYSSPLPDIFQPNLA 
Sbjct: 601 SSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGG-----GYSSPLPDIFQPNLAA 660

Query: 661 QPSSSVMNSSKKEDTRAFDFISEHIAAARDPKRVV 688
           Q  SSVMNSSKKEDTRAFDFIS+H+AAARDPKRVV
Sbjct: 661 QSPSSVMNSSKKEDTRAFDFISDHVAAARDPKRVV 685

BLAST of Cp4.1LG02g14600 vs. ExPASy TrEMBL
Match: A0A6J1GWW5 (VHS domain-containing protein At3g16270-like OS=Cucurbita moschata OX=3662 GN=LOC111457592 PE=4 SV=1)

HSP 1 Score: 1115 bits (2885), Expect = 0.0
Identity = 588/695 (84.60%), Postives = 619/695 (89.06%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE
Sbjct: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           HKSPIVKQKALRL KYAVGKSGVEF REMQRHSVAVRQL HYKGQPDPLKGDALNKAVR+
Sbjct: 61  HKSPIVKQKALRLIKYAVGKSGVEFRREMQRHSVAVRQLFHYKGQPDPLKGDALNKAVRE 120

Query: 121 TAQDAISAIFAEEDNRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQG 180
           TA DAISAIFAEEDN+PAPSENLN RIQGFGNSNYEPP EDKKSFLSEVVGLGSASIK G
Sbjct: 121 TAHDAISAIFAEEDNKPAPSENLNRRIQGFGNSNYEPPPEDKKSFLSEVVGLGSASIKHG 180

Query: 181 LSNLAQGHSSRKNGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRETLGTSKSTISGPWNP 240
           LSN AQGHSSRKNGTS  RGPNLQRSLTTE+EYDNRYEPVEYGRETLGTSKST SG WN 
Sbjct: 181 LSNFAQGHSSRKNGTSSPRGPNLQRSLTTEIEYDNRYEPVEYGRETLGTSKSTTSGTWNQ 240

Query: 241 DSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAKLDAM 300
           DS       +NGN SSGSS  KTREERLLETIATAGGVR+QPTRDAIQAFLVEAAKLDA+
Sbjct: 241 DS-----RVSNGNSSSGSSVSKTREERLLETIATAGGVRLQPTRDAIQAFLVEAAKLDAL 300

Query: 301 ALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSESPQA 360
            LS ALE+KLKSPSWQVRFKALC+LESIVR+  D+HFSIV SYFSENQDAVIGCSESPQA
Sbjct: 301 VLSHALETKLKSPSWQVRFKALCILESIVRRSGDEHFSIVTSYFSENQDAVIGCSESPQA 360

Query: 361 SLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDAG-------VLEV 420
           SLR+KASKVMPLLDGGKGVP+M DSEKS P+N SSTIQMPDL+DTSDAG        LEV
Sbjct: 361 SLRDKASKVMPLLDGGKGVPTMTDSEKSLPSNTSSTIQMPDLLDTSDAGDYGGTDKSLEV 420

Query: 421 ENLSKTPLVDDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSGMNVDNGQ 480
           ENLS  PLVDDLFG G+NTVTSTSELKNDDDPFSDV F TT+  ENPDDLFSGMN +N Q
Sbjct: 421 ENLSSVPLVDDLFGVGLNTVTSTSELKNDDDPFSDVLFHTTETRENPDDLFSGMNFENNQ 480

Query: 481 VSNENKRPASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALKSNDIGDS 540
           V++ENK+P SEQKNE GVF +FGSSSE AVQEH RKDV DLMSGLSIHE+ALK+ D GDS
Sbjct: 481 VTDENKKPTSEQKNEPGVFDIFGSSSEPAVQEHARKDVIDLMSGLSIHEDALKNKDKGDS 540

Query: 541 KDSLSESLYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPGMTYLPSGMMFNPAF 600
           KDSLSESL+SVSSQPNHQ Q    S+ G YSSPMVGTNMNA + PGM YLPSGMMFNPAF
Sbjct: 541 KDSLSESLFSVSSQPNHQNQVPHDSLTGTYSSPMVGTNMNATYFPGMPYLPSGMMFNPAF 600

Query: 601 SSQPMGYAPTGNFFAQQNLVSAMSNYQQFGNPHLQSSGGSAGNGGYSSPLPDIFQPNLAT 660
           SSQPMGYAPTGNFF QQ L+SAMSNYQQFGNP+LQSSGG     GYSSPLPDIFQPNLA 
Sbjct: 601 SSQPMGYAPTGNFFTQQQLLSAMSNYQQFGNPNLQSSGG-----GYSSPLPDIFQPNLAA 660

Query: 661 QPSSSVMNSSKKEDTRAFDFISEHIAAARDPKRVV 688
           Q  SS+MNSSKKEDTRAFDFIS+H+AAARDPKRVV
Sbjct: 661 QSPSSMMNSSKKEDTRAFDFISDHVAAARDPKRVV 685

BLAST of Cp4.1LG02g14600 vs. TAIR 10
Match: AT3G16270.1 (ENTH/VHS family protein )

HSP 1 Score: 667.2 bits (1720), Expect = 1.5e-191
Identity = 398/708 (56.21%), Postives = 498/708 (70.34%), Query Frame = 0

Query: 1   MDSSRRAVESYWRSRMIDAATSDEDKVTPVYKLEEICEVLRSSHVSIVKEFSEFILKRLE 60
           MD+SRRAVESYWRSRMIDA TSDEDKV PVYKLEEIC++LRSSHVSIVKEFSEFILKRL+
Sbjct: 1   MDTSRRAVESYWRSRMIDAVTSDEDKVAPVYKLEEICDLLRSSHVSIVKEFSEFILKRLD 60

Query: 61  HKSPIVKQKALRLTKYAVGKSGVEFGREMQRHSVAVRQLLHYKGQPDPLKGDALNKAVRD 120
           +KSPIVKQKALRL KYAVGKSG EF REMQR+SVAVR L HYKG PDPLKGDALNKAVR+
Sbjct: 61  NKSPIVKQKALRLIKYAVGKSGSEFRREMQRNSVAVRNLFHYKGHPDPLKGDALNKAVRE 120

Query: 121 TAQDAISAIFAEED-NRPAPSENLNSRIQGFGNSNYEPPAEDKKSFLSEVVGLGSASIKQ 180
           TA + ISAIF+EE+  +PA  E++N RI+GFGN+N++ P+ D KSFLSEVVG+GSASIKQ
Sbjct: 121 TAHETISAIFSEENGTKPAAPESINRRIEGFGNTNFQVPSNDNKSFLSEVVGIGSASIKQ 180

Query: 181 GLSNLAQGHSSRK--NGTSGHRGPNLQRSLTTEMEYDNRYEPVEYGRE-TLGTSKSTISG 240
           G+SN AQGH  +K  NG+S +RGPNL RSLT E E  +RY+PV+ G++   GTSK+T  G
Sbjct: 181 GISNFAQGHLPKKNENGSSSYRGPNLHRSLTMENENFSRYDPVKLGKDGNYGTSKNTTGG 240

Query: 241 PWNPDSWANKVEATNGNLSSGSSERKTREERLLETIATAGGVRIQPTRDAIQAFLVEAAK 300
                SW +     + + +S   E KTREE+LLETI T+GGVR+QPTRDA+  F++EAAK
Sbjct: 241 -----SWGHASGEASESSASVRVESKTREEKLLETIVTSGGVRLQPTRDALHVFILEAAK 300

Query: 301 LDAMALSSALESKLKSPSWQVRFKALCVLESIVRKKDDDHFSIVASYFSENQDAVIGCSE 360
           +DA+ALS AL+ KL SP WQVR KALCVLE+I+RKK+D++FSIV +YFSEN DA+  C+E
Sbjct: 301 MDAVALSIALDGKLHSPMWQVRMKALCVLEAILRKKEDENFSIVHTYFSENLDAIQRCAE 360

Query: 361 SPQASLREKASKVMPLLDGGKGVPSMNDSEKSRPNNPSSTIQMPDLIDTSDA-------G 420
           SPQ+SLREKA+KV+ LL+GG+    M+ S+ +      + + +PDLIDT D+        
Sbjct: 361 SPQSSLREKANKVLSLLNGGQSSGLMSSSDNTVKR--EAAVDLPDLIDTGDSDDTLNNLN 420

Query: 421 VLEVENLSKT--PLV-DDLFGDGVNTVTSTSELKNDDDPFSDVSFQTTDNGENPDDLFSG 480
            ++  +   T  PL+ DD FGD  +   S+SE K DDDPF+DVSF   +  E+ DDLFSG
Sbjct: 421 AIDTGSTVATAGPLMDDDWFGDSSDIGLSSSEKKTDDDPFADVSFHPNEEKESADDLFSG 480

Query: 481 MNVDNGQVSNENKRPASEQKNEHGVFYVFGSSSEAAVQEHTRKDVNDLMSGLSIHENALK 540
           M V         K  A    +   +F +FGS+++   +    K++NDLM   SI EN   
Sbjct: 481 MTVG-------EKSAAVGGNHVPDLFDMFGSTAKLEAEPKDAKNINDLMGSFSIDEN--N 540

Query: 541 SNDIGDSKDSLSESLYSVSSQPNHQYQTKDSSVNGIYSSPMVGTNMNAAFLPG--MTY-L 600
           SN  G S  +L + L+++ S  +H  Q  ++ V GI  S   G   N   LPG  M +  
Sbjct: 541 SNQKGSSSSTLPQDLFAMPSTTSH--QAPENPVGGILGSQNPGFIQN-TMLPGGVMPFNF 600

Query: 601 PSGMMFNPAFSSQPMGYAPTGNFFA-QQNLVSAMSNYQQFGNPHLQSSGG--SAG-NGGY 660
           P GMM NPAF+SQP+ YA   +  A QQ  +  MSN+QQFGN + Q SG   S G +GG 
Sbjct: 601 PQGMMMNPAFASQPLNYAAMASLLAQQQQYLGNMSNFQQFGNLNAQGSGNVLSMGTSGGN 660

Query: 661 SSPLPDIFQPNLATQPSSSVMNSSKKEDTRAFDFISEHIAAARDPKRV 688
            S LPDIFQPN   Q  +S MN SKKEDTRAFDFIS+H+ +ARD KRV
Sbjct: 661 QSALPDIFQPNFGNQAPTSTMNGSKKEDTRAFDFISDHLTSARDTKRV 689

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C5H42.1e-19056.21Protein MODIFIED TRANSPORT TO THE VACUOLE 1 OS=Arabidopsis thaliana OX=3702 GN=M... [more]
G3V8Y75.2e-0831.97AP-4 complex accessory subunit Tepsin OS=Rattus norvegicus OX=10116 GN=Tepsin PE... [more]
Q3U3N66.3e-0624.79AP-4 complex accessory subunit Tepsin OS=Mus musculus OX=10090 GN=Tepsin PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_023524865.10.0100.00VHS domain-containing protein At3g16270-like [Cucurbita pepo subsp. pepo][more]
KAG6606757.10.097.24Protein MODIFIED TRANSPORT TO THE VACUOLE 1, partial [Cucurbita argyrosperma sub... [more]
XP_022949030.10.097.24VHS domain-containing protein At3g16270-like [Cucurbita moschata][more]
XP_022997847.10.096.80VHS domain-containing protein At3g16270-like [Cucurbita maxima][more]
KAG7036470.10.089.97VHS domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
Match NameE-valueIdentityDescription
A0A6J1GBM70.097.24VHS domain-containing protein At3g16270-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1K6680.096.80VHS domain-containing protein At3g16270-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3BH260.085.06VHS domain-containing protein At3g16270 OS=Cucumis melo OX=3656 GN=LOC103489958 ... [more]
A0A6J1IW710.085.18VHS domain-containing protein At3g16270-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1GWW50.084.60VHS domain-containing protein At3g16270-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT3G16270.11.5e-19156.21ENTH/VHS family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002014VHS domainSMARTSM00288VHS_2coord: 13..149
e-value: 5.3E-24
score: 95.7
IPR002014VHS domainPROSITEPS50179VHScoord: 20..89
score: 9.286965
IPR008942ENTH/VHSGENE3D1.25.40.90coord: 2..140
e-value: 4.4E-54
score: 184.4
IPR008942ENTH/VHSSUPERFAMILY48464ENTH/VHS domaincoord: 15..134
IPR013809ENTH domainPFAMPF01417ENTHcoord: 16..133
e-value: 4.6E-5
score: 23.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 435..487
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 435..480
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 646..664
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 384..401
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..159
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 373..401
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 138..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..212
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 624..664
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 184..210
IPR039273AP-4 complex accessory subunit TepsinPANTHERPTHR21514UNCHARACTERIZEDcoord: 1..681
IPR035802Tepsin, ENTH/VHS domainCDDcd03572ENTH_like_Tepsincoord: 13..132
e-value: 3.41877E-48
score: 163.495

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g14600.1Cp4.1LG02g14600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0030136 clathrin-coated vesicle
cellular_component GO:0032588 trans-Golgi network membrane
molecular_function GO:0035091 phosphatidylinositol binding
molecular_function GO:0043130 ubiquitin binding