Cp4.1LG02g14440 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g14440
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionATP-dependent 6-phosphofructokinase
LocationCp4.1LG02: 13977031 .. 13980080 (+)
RNA-Seq ExpressionCp4.1LG02g14440
SyntenyCp4.1LG02g14440
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTTTTTTTTTTTTTACTTCTTCTACAATCCACCAGACGCCATGACCTTTGATACCCATTTTCAAATTCAGCAAGTCACCACCATCTGACATCGCCGCCGATCAGCCTACCCCACCACCGTCCTCTGCTTTAAATATATGCCTCCCCTTCTTCCTTCTTCTTTGACCTTCTCCAGTTTCCGTTTCTAACTTCTCTTCTCCTACTTTCCTTTGACTTCTGCTGCATCACCGCTTTCACAACAGTGGATCATCTCTGATGGCTTGTAATGGCGTTAATGGAGATTCCCTCGGTAACGGCTTTCATTATGTTCTTGAAGATGTCCCTCACTTGACTGATTATCTCCCTGACCTTCCGGTAACCCTCTCTCTTCTCTGTTTGTTTCTTCCAAAATTTCAGACATGTCTTAATCCTTCATCCATGCTGTAAAATCTACTTTGATTCCATGTTTTTGTCGTTTTTCCCTTCCATCCCACTTGTAATTTTATCTGCTTGTTTTCAGGGGTATCCTAATCCTTTACAAGATAATCCTGCCTATTCTGCAGTCAAGTATGTTCCTTCTCTGTAGCCCGTTACACAGTTTTTCTCTATCCATCCATCCATAAACTAACCAGAATTTTGTGTTCATCAGGCAATATTTTGTTAACGTGGACGATGCTGTTGCTCAAAATGTAAGCGACCCATTTCTAACTTCACAGTTTGAGATCAATCTGTTCTAAGATTTTGTTCAAAACAGGTTGTTGTTCAGAACAATAGTCCTAGAGGGACACATTTTCGACGAGCTGGACCTCGACAAAAGGTTAAACATATGTTTCTTCATCTTCATTTCCCCCCCTACAATCTCTGTCATCATCGTAATCAAAGTTCAACACTTTTTTCAGGTGTTTTTTGAATCCGATGAAGTTCATGCGTGCATTGTAACTTGTGGTGGTTTGTGTCCGGGTCTTAATACAGTGATCAGGGAAATAGTATGTGGATTGTACCACATGTATGGCGTCCGCACAGTTGTTGGAATTGAGGTGATTATAGACGCTTTGAACTTTCCTTAATCACCATTTGATGTCATCACTAAAACGCCCAGTTGTTTCACCTAATAACATATAGGCAGGGTACAAAGGCTTCTACGCTAGGAATACAATTCCTTTAACACCAAAAATGGTGAACAACATCCATAAACGTGGAGGCACCATCCTTGGGACCTCAAGAGGTGGCCACAATACCACCAAGATTGTTGACAGCATTCAACATAGAGGCATCAATCAGGTCTATATACTTGGAGGGGATGGAACCCAGAAAGGAGCTGCTGCTATTTTTGAGGTAATCCACATTGGTTTGGCTAATTGCAGGTGGGTTAGTGTGATATCGCACGTTGGTTGGGGAGGAGAACGAAACACTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACGCGTTTTAAAAACCTTGAGGAAAAGCCTGAAAGGAAAAGCCTAAAAAGGACAATATCTGCTAGCGGTGGACTTGGGCTGTTACAGTTAGATTTTGTATTTTGTATTTGTATTTTGTATTTGTATTGTATGTCATAAACTTATGTTTCTCATTCATGCAGGAAATTAAGAGGCGTGGCCTCAAAGTTGCGGTAGCCGGAATTCCAAAGACCATCGATAACGACATTCCAGTAATAATACAGTAAACTTGTTTGTTGATCACTTATGAACATTGATGCAAGTCCAAATTCTATCTTATTTCAGGTCATTGACAAGTCATTTGGTTTTGACACTGCCGTTGAAGAAGCTCAACGTGCCATTAGTGCTGCTCATGTTGAATCAGAAAGTAATGATAATTGCATAGGTTTGGTCAAGTTGATGGGTCGCAACAGTGGTAAGCTTCAATCATTTCATCACTAGTTGGGGTGTGAAATCGTGACCAAAATGTGTATTATTAACCATTCCCTTCTTTGTCAAAGGGTTTATTGCAATGTATGCAACTCTCGCAAGCAGAGATGTTGATTGCTGCTTGATACCAGAGTCACCCTTTTATCTTGAAGGAGAGGGAGGCCTCTTCGAATATATTGAGCATTGTATAGAAGAACAAGGACACATGGTTATAGTGATAGCTGAAGGGGCAGGACAAGAACTTATTTTGAAAAGCATGCATAAACTAGATGAACAAGATGCTTCGGGAAATAAGCTACACCAGGATGTTGGGTTATGGATATCCCAACAAATCAAAGTTCGTCCTCCTCTTTAACTCTACCAAATACTCATTGATTTAGATAGAGATTAACAAAGAGTTTTTGTTGATCCAGGACTACTTCAAAAAAAAGAAGAAGATTACAATAAATCTCAAATACATAGATCCTACTTACATGATTCGTGCAGTTCCCAGCATTGCATCAGATAACATATTCTGCACCCTGCTTGCTCATAGTGCGATTCACGGTGCAATGGCGGGATACACAGGATTCACAGTAGGGCCTGTAAACGGGAGACATGCTTACATACCATTTAATGTGAGGCCAATATCATATACTTTACTTTACTTCTGCTATAGTTACATAAAAAAGAGTCTCAGTAGCAGAACATAGAGCAAACTAAGCAGCCAAGTTTATGATATTTGCAGCGGATCATTGAAAGACAGAACAAAGTAGTGATTACCGACAGGATGTGGGCAAGGCTGCTGTCTTCAACCAACCAGCCAAGTTTCTTGCACCCTAAACATATGGATGGCGAGCAGGAGAATGGTAAGAATCCACAACTCAATGGGTAAGCAGTTCTACAGAAGAGAAGAAGAAGTTATCCCCACACTTGTACTCTAATTTACTTACAACTGTACTACCTCCTAGAGATGAACTCAATTATTCTATAGGTTGAGCTTGGTATTATTAAACCAGCCAAATTTGTTGAGATGTCGAGATGGAAAATAAGGTCAGATGGGATCATCTGCCCCCACGAACTTCGACTTGTTCCATAGCTCATGTGCTGTAATAAAATAGTCTATGTTCTTGACGGTCTTTCATGAACTAATGTATGTTCCCATACTAGTTTATAATAAGAGAATAAATCGTCTT

mRNA sequence

TTTTTTTTTTTTTTTTTTACTTCTTCTACAATCCACCAGACGCCATGACCTTTGATACCCATTTTCAAATTCAGCAAGTCACCACCATCTGACATCGCCGCCGATCAGCCTACCCCACCACCGTCCTCTGCTTTAAATATATGCCTCCCCTTCTTCCTTCTTCTTTGACCTTCTCCAGTTTCCGTTTCTAACTTCTCTTCTCCTACTTTCCTTTGACTTCTGCTGCATCACCGCTTTCACAACAGTGGATCATCTCTGATGGCTTGTAATGGCGTTAATGGAGATTCCCTCGGTAACGGCTTTCATTATGTTCTTGAAGATGTCCCTCACTTGACTGATTATCTCCCTGACCTTCCGGGGTATCCTAATCCTTTACAAGATAATCCTGCCTATTCTGCAGTCAAGCAATATTTTGTTAACGTGGACGATGCTGTTGCTCAAAATGTTGTTGTTCAGAACAATAGTCCTAGAGGGACACATTTTCGACGAGCTGGACCTCGACAAAAGGTGTTTTTTGAATCCGATGAAGTTCATGCGTGCATTGTAACTTGTGGTGGTTTGTGTCCGGGTCTTAATACAGTGATCAGGGAAATAGTATGTGGATTGTACCACATGTATGGCGTCCGCACAGTTGTTGGAATTGAGGCAGGGTACAAAGGCTTCTACGCTAGGAATACAATTCCTTTAACACCAAAAATGGTGAACAACATCCATAAACGTGGAGGCACCATCCTTGGGACCTCAAGAGGTGGCCACAATACCACCAAGATTGTTGACAGCATTCAACATAGAGGCATCAATCAGGAAATTAAGAGGCGTGGCCTCAAAGTTGCGGTAGCCGGAATTCCAAAGACCATCGATAACGACATTCCAGTCATTGACAAGTCATTTGGTTTTGACACTGCCGTTGAAGAAGCTCAACGTGCCATTAGTGCTGCTCATGTTGAATCAGAAAGTAATGATAATTGCATAGGTTTGGTCAAGTTGATGGGTCGCAACAGTGGGTTTATTGCAATGTATGCAACTCTCGCAAGCAGAGATGTTGATTGCTGCTTGATACCAGAGTCACCCTTTTATCTTGAAGGAGAGGGAGGCCTCTTCGAATATATTGAGCATTGTATAGAAGAACAAGGACACATGGTTATAGTGATAGCTGAAGGGGCAGGACAAGAACTTATTTTGAAAAGCATGCATAAACTAGATGAACAAGATGCTTCGGGAAATAAGCTACACCAGGATGTTGGGTTATGGATATCCCAACAAATCAAAGACTACTTCAAAAAAAAGAAGAAGATTACAATAAATCTCAAATACATAGATCCTACTTACATGATTCGTGCAGTTCCCAGCATTGCATCAGATAACATATTCTGCACCCTGCTTGCTCATAGTGCGATTCACGGTGCAATGGCGGGATACACAGGATTCACAGTAGGGCCTGTAAACGGGAGACATGCTTACATACCATTTAATCGGATCATTGAAAGACAGAACAAAGTAGTGATTACCGACAGGATGTGGGCAAGGCTGCTGTCTTCAACCAACCAGCCAAGTTTCTTGCACCCTAAACATATGGATGGCGAGCAGGAGAATGGTAAGAATCCACAACTCAATGGGTAAGCAGTTCTACAGAAGAGAAGAAGAAGTTATCCCCACACTTGTACTCTAATTTACTTACAACTGTACTACCTCCTAGAGATGAACTCAATTATTCTATAGGTTGAGCTTGGTATTATTAAACCAGCCAAATTTGTTGAGATGTCGAGATGGAAAATAAGGTCAGATGGGATCATCTGCCCCCACGAACTTCGACTTGTTCCATAGCTCATGTGCTGTAATAAAATAGTCTATGTTCTTGACGGTCTTTCATGAACTAATGTATGTTCCCATACTAGTTTATAATAAGAGAATAAATCGTCTT

Coding sequence (CDS)

ATGGCTTGTAATGGCGTTAATGGAGATTCCCTCGGTAACGGCTTTCATTATGTTCTTGAAGATGTCCCTCACTTGACTGATTATCTCCCTGACCTTCCGGGGTATCCTAATCCTTTACAAGATAATCCTGCCTATTCTGCAGTCAAGCAATATTTTGTTAACGTGGACGATGCTGTTGCTCAAAATGTTGTTGTTCAGAACAATAGTCCTAGAGGGACACATTTTCGACGAGCTGGACCTCGACAAAAGGTGTTTTTTGAATCCGATGAAGTTCATGCGTGCATTGTAACTTGTGGTGGTTTGTGTCCGGGTCTTAATACAGTGATCAGGGAAATAGTATGTGGATTGTACCACATGTATGGCGTCCGCACAGTTGTTGGAATTGAGGCAGGGTACAAAGGCTTCTACGCTAGGAATACAATTCCTTTAACACCAAAAATGGTGAACAACATCCATAAACGTGGAGGCACCATCCTTGGGACCTCAAGAGGTGGCCACAATACCACCAAGATTGTTGACAGCATTCAACATAGAGGCATCAATCAGGAAATTAAGAGGCGTGGCCTCAAAGTTGCGGTAGCCGGAATTCCAAAGACCATCGATAACGACATTCCAGTCATTGACAAGTCATTTGGTTTTGACACTGCCGTTGAAGAAGCTCAACGTGCCATTAGTGCTGCTCATGTTGAATCAGAAAGTAATGATAATTGCATAGGTTTGGTCAAGTTGATGGGTCGCAACAGTGGGTTTATTGCAATGTATGCAACTCTCGCAAGCAGAGATGTTGATTGCTGCTTGATACCAGAGTCACCCTTTTATCTTGAAGGAGAGGGAGGCCTCTTCGAATATATTGAGCATTGTATAGAAGAACAAGGACACATGGTTATAGTGATAGCTGAAGGGGCAGGACAAGAACTTATTTTGAAAAGCATGCATAAACTAGATGAACAAGATGCTTCGGGAAATAAGCTACACCAGGATGTTGGGTTATGGATATCCCAACAAATCAAAGACTACTTCAAAAAAAAGAAGAAGATTACAATAAATCTCAAATACATAGATCCTACTTACATGATTCGTGCAGTTCCCAGCATTGCATCAGATAACATATTCTGCACCCTGCTTGCTCATAGTGCGATTCACGGTGCAATGGCGGGATACACAGGATTCACAGTAGGGCCTGTAAACGGGAGACATGCTTACATACCATTTAATCGGATCATTGAAAGACAGAACAAAGTAGTGATTACCGACAGGATGTGGGCAAGGCTGCTGTCTTCAACCAACCAGCCAAGTTTCTTGCACCCTAAACATATGGATGGCGAGCAGGAGAATGGTAAGAATCCACAACTCAATGGGTAA

Protein sequence

MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQEIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG
Homology
BLAST of Cp4.1LG02g14440 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.2e-193
Identity = 335/446 (75.11%), Postives = 377/446 (84.53%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           YVLEDVPHL+DYLP LP YPNPLQDNPAYS VKQYFV+ DD+V Q +VV  + PRG HFR
Sbjct: 18  YVLEDVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDGPRGIHFR 77

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPRQKV+FESDEVHACIVTCGGLCPGLNTVIREIV  L +MYGV+ ++GI+ GY+GFY
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           A+NT+ L  K+VN+IHKRGGTILGTSRGGH+TTKIVDSIQ RGINQ              
Sbjct: 138 AKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGAS 197

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               EI+RRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHVE+ES +N I
Sbjct: 198 VIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGI 257

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           G+VKLMGR SGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIE  ++E GHMV+VI
Sbjct: 258 GVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVI 317

Query: 317 AEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTYM 376
           AEGAGQ+L+ KSM  +  +DASGNKL +DVGLW+SQ IKD+F +KK + +NLKYIDPTYM
Sbjct: 318 AEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHFNQKKMV-MNLKYIDPTYM 377

Query: 377 IRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVITD 436
           IRAVPS ASDN++CTLLA SA+HGAMAGYTG+  G VNGR  YIPF RI E+QN VVITD
Sbjct: 378 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITD 437

Query: 437 RMWARLLSSTNQPSFLHPKHMDGEQE 445
           RMWARLLSSTNQPSFL PK +   +E
Sbjct: 438 RMWARLLSSTNQPSFLGPKDVFDNKE 462

BLAST of Cp4.1LG02g14440 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 2.5e-192
Identity = 332/446 (74.44%), Postives = 374/446 (83.86%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           Y+L+DVPHL DYLPDLP YPNPLQDNPAYS VKQYFV+ DD+V + VVV  + PRG HFR
Sbjct: 18  YILQDVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVVVHKDGPRGVHFR 77

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPRQKV+FESDEVHACIVTCGGLCPGLNTVIRE+V  L +MYGV+ ++GI+ GY+GFY
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           A+NTIPL  K+VN+IHKRGGTI+GTSRGGH+T KIVDSIQ RGINQ              
Sbjct: 138 AKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGAS 197

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               EI+RR LKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHVE+ESN+N I
Sbjct: 198 VIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGI 257

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           G VKLMGR SG+IAMYATLASRDVDCCLIPESPFYLEGEGGLFE+IE  +++ GHMVIV+
Sbjct: 258 GFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVL 317

Query: 317 AEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTYM 376
           AEGAGQ+L+ KSM      DASGNKL +DVGLW+SQ IKD+FKK K + +NLKYIDPTYM
Sbjct: 318 AEGAGQDLMCKSMES-TPMDASGNKLLKDVGLWLSQSIKDHFKKNKMV-MNLKYIDPTYM 377

Query: 377 IRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVITD 436
           IRAVPS ASDN++CTLLA SA+HGAMAGYTG+T G VNGR  YIPF RI E QN VVITD
Sbjct: 378 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITD 437

Query: 437 RMWARLLSSTNQPSFLHPKHMDGEQE 445
           RMWARLLSSTNQPSFL PK    E++
Sbjct: 438 RMWARLLSSTNQPSFLGPKDTSEEKK 461

BLAST of Cp4.1LG02g14440 vs. ExPASy Swiss-Prot
Match: Q9M0F9 (ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE=1 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 4.5e-186
Identity = 325/437 (74.37%), Postives = 368/437 (84.21%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           Y+LEDVPH +D  PD P YPNPLQDN AYS VKQYFV+ DD V Q +VV  +SPRGTHFR
Sbjct: 19  YILEDVPHFSDDFPDHPTYPNPLQDNAAYSVVKQYFVDEDDTVPQKIVVHPDSPRGTHFR 78

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPRQ+V+FESD+V ACIVTCGGLCPGLNTVIREIVCGL +MYGV+ ++GI+ GY+GFY
Sbjct: 79  RAGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVKRILGIDGGYRGFY 138

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           ARNTI L  K VN+IH+ GGTILGTSRGGHNTTKIVDSIQ RGINQ              
Sbjct: 139 ARNTIHLDLKTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKGAA 198

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               EI++R LKVAVAGIPKTIDNDIP+ID+SFGFDTAVEEAQRAI+AAHVE+ S +N I
Sbjct: 199 AIFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAINAAHVEATSFENGI 258

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           GLVKLMGR SGFIAM+ATLASRDVDCCLIPESPF+LEG GGLFE+I+  ++E GHMVIVI
Sbjct: 259 GLVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFIDKRLKESGHMVIVI 318

Query: 317 AEGAGQELILKSMHKLDE-QDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTY 376
           AEGAGQ+L+ +SM +    +DASGNKL QD+GLWISQ+IKD+F   KK+T+ LKYIDPTY
Sbjct: 319 AEGAGQDLLSESMKESTTLKDASGNKLLQDIGLWISQRIKDHF--AKKMTLTLKYIDPTY 378

Query: 377 MIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVIT 435
           MIRAVPS ASDN+ CTLLA SA+HG MAGY GFTVG VNGRH YIPFNRI E+QNKVVIT
Sbjct: 379 MIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNRITEKQNKVVIT 438

BLAST of Cp4.1LG02g14440 vs. ExPASy Swiss-Prot
Match: Q9M076 (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE=1 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 5.9e-186
Identity = 324/456 (71.05%), Postives = 377/456 (82.68%), Query Frame = 0

Query: 1   MACNGVNGD-SLGNG-FHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDA 60
           MA NGV+    L  G   YVLEDVPHL+DY+ DLP YPNPLQ N AYS V+QYFV+ DD 
Sbjct: 1   MASNGVDEQIKLVEGPAGYVLEDVPHLSDYILDLPTYPNPLQSNAAYSVVRQYFVDEDDT 60

Query: 61  VAQNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYH 120
           V + +VV  +SPRGTHFRRAGPRQKV+F+  +V ACIVTCGGLCPGLNTVIREIVCGL+ 
Sbjct: 61  VQEKIVVHKDSPRGTHFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHF 120

Query: 121 MYGVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHR 180
           MYGV  V+G++ G++GFY++NT+ LTPK V++IHKRGGTILGTSRGGH+T+KIVD+IQ R
Sbjct: 121 MYGVTEVIGVDCGFRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDR 180

Query: 181 GINQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEA 240
            INQ                  EI+RRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEA
Sbjct: 181 EINQVYIIGGDGTQKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEA 240

Query: 241 QRAISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGL 300
           QRAI+AAHVE+ S +N IG+VKLMGR SGFIAMYATLASRDVDCCLIPESPFYLEG+GGL
Sbjct: 241 QRAINAAHVEATSVENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGL 300

Query: 301 FEYIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYF 360
           +E+I   + E GHMVIVIAEGAGQ+L+ +S   +++QDASGNKL +DVGLW+S +IK+YF
Sbjct: 301 YEFIAKRLRENGHMVIVIAEGAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYF 360

Query: 361 KKKKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHA 420
            K   + I LKYIDPTYMIRA+P+ ASDN++ TLLA SA+HGAMAGYTGF  G VNGRH 
Sbjct: 361 AKHNVMDITLKYIDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHT 420

Query: 421 YIPFNRIIERQNKVVITDRMWARLLSSTNQPSFLHP 437
           YIPFNRI ERQNKVVITDRMWAR+LSSTNQPSF++P
Sbjct: 421 YIPFNRITERQNKVVITDRMWARMLSSTNQPSFMNP 453

BLAST of Cp4.1LG02g14440 vs. ExPASy Swiss-Prot
Match: Q9FKG3 (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK4 PE=1 SV=1)

HSP 1 Score: 630.9 bits (1626), Expect = 1.1e-179
Identity = 303/436 (69.50%), Postives = 365/436 (83.72%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           +VLEDVPHLT +LPDLP YPNPL+++ AY+ VK+ FV+ +D VAQN+VVQ  S RG HFR
Sbjct: 69  FVLEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGVHFR 128

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPR++V+F SDEV ACIVTCGGLCPG+NTVIREIVCGL +MYGV  ++GI+ GY+GFY
Sbjct: 129 RAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFY 188

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           ++NT+ LTPK+VN+IHKRGGT L TSRGGH+T KIVD+IQ RGINQ              
Sbjct: 189 SKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAE 248

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               E++RRGL+VAV+GIPKTIDNDI VIDKSFGFDTAVEEAQRAI+AAHVE ES +N +
Sbjct: 249 KIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGV 308

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           G+VKLMGR SGFIAM ATLA+RDVDCCLIPESPF+LEG+GGLFE+IE  ++E  HMVIVI
Sbjct: 309 GIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVI 368

Query: 317 AEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTYM 376
           AEGAGQ+ + +SM   + +DASGN+L  DVGLW++QQIKD+F   +K+ IN+KYIDPTYM
Sbjct: 369 AEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYIDPTYM 428

Query: 377 IRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVITD 435
           IRA+PS ASDN++CTLLA SA+HGAMAGY+GFTVGPVN RHAYIP +++ E  N V +TD
Sbjct: 429 IRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTD 488

BLAST of Cp4.1LG02g14440 vs. NCBI nr
Match: XP_023524893.1 (ATP-dependent 6-phosphofructokinase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919 bits (2375), Expect = 0.0
Identity = 453/471 (96.18%), Postives = 453/471 (96.18%), Query Frame = 0

Query: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60
           MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA
Sbjct: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60

Query: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120
           QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY
Sbjct: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120

Query: 121 GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180
           GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI
Sbjct: 121 GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180

Query: 181 NQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240
           NQ                  EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR
Sbjct: 181 NQVYILGGDGTQKGAAAIFEEIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240

Query: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300
           AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE
Sbjct: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300

Query: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360
           YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK
Sbjct: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360

Query: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420
           KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI
Sbjct: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420

Query: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG 453
           PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG
Sbjct: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG 471

BLAST of Cp4.1LG02g14440 vs. NCBI nr
Match: KAG6606740.1 (ATP-dependent 6-phosphofructokinase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036455.1 ATP-dependent 6-phosphofructokinase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 915 bits (2365), Expect = 0.0
Identity = 451/471 (95.75%), Postives = 451/471 (95.75%), Query Frame = 0

Query: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60
           MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA
Sbjct: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60

Query: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120
           QNVVVQNNSPRGTHFRRAGPRQKV FESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY
Sbjct: 61  QNVVVQNNSPRGTHFRRAGPRQKVLFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120

Query: 121 GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180
           GVRTVVGIE GYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI
Sbjct: 121 GVRTVVGIEGGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180

Query: 181 NQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240
           NQ                  EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR
Sbjct: 181 NQVYILGGDGTQKGAAAIFEEIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240

Query: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300
           AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE
Sbjct: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300

Query: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360
           YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK
Sbjct: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360

Query: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420
           KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI
Sbjct: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420

Query: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG 453
           PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG
Sbjct: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG 471

BLAST of Cp4.1LG02g14440 vs. NCBI nr
Match: XP_022997851.1 (ATP-dependent 6-phosphofructokinase 3-like [Cucurbita maxima])

HSP 1 Score: 913 bits (2359), Expect = 0.0
Identity = 449/471 (95.33%), Postives = 451/471 (95.75%), Query Frame = 0

Query: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60
           MACNGVNGDS+GNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA
Sbjct: 1   MACNGVNGDSIGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60

Query: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120
           QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY
Sbjct: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120

Query: 121 GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180
           GVRTVVGIE GYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI
Sbjct: 121 GVRTVVGIEGGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180

Query: 181 NQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240
           NQ                  EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR
Sbjct: 181 NQVYILGGDGTQKGAAAIFEEIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240

Query: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300
           AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE
Sbjct: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300

Query: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360
           YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK
Sbjct: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360

Query: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420
           KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI
Sbjct: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420

Query: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG 453
           PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHM GE+ENGKNPQLNG
Sbjct: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMGGEEENGKNPQLNG 471

BLAST of Cp4.1LG02g14440 vs. NCBI nr
Match: XP_022948872.1 (ATP-dependent 6-phosphofructokinase 3-like [Cucurbita moschata])

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 449/470 (95.53%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60
           MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA
Sbjct: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60

Query: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120
           QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY
Sbjct: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120

Query: 121 GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180
           GVRTVVGIE GYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI
Sbjct: 121 GVRTVVGIEGGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180

Query: 181 NQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240
           NQ                  EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR
Sbjct: 181 NQVYILGGDGTQKGAAAIFEEIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240

Query: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300
           AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE
Sbjct: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300

Query: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360
           YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK
Sbjct: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360

Query: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420
           KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI
Sbjct: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420

Query: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLN 452
           PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHM GE+ENGKNPQLN
Sbjct: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMGGEEENGKNPQLN 470

BLAST of Cp4.1LG02g14440 vs. NCBI nr
Match: XP_008447566.1 (PREDICTED: ATP-dependent 6-phosphofructokinase 6-like [Cucumis melo])

HSP 1 Score: 817 bits (2111), Expect = 6.31e-296
Identity = 411/472 (87.08%), Postives = 427/472 (90.47%), Query Frame = 0

Query: 1   MACNGVNG-----DSL-GNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVN 60
           M CNG NG     DSL G  +HYVLEDVPHLTDYLPDLP   NPLQD+PAYSAVKQYFVN
Sbjct: 36  MDCNGGNGRDALGDSLTGGDYHYVLEDVPHLTDYLPDLPANLNPLQDHPAYSAVKQYFVN 95

Query: 61  VDDAVAQNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVC 120
           VDDAVAQNVVV NNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVC
Sbjct: 96  VDDAVAQNVVVHNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVC 155

Query: 121 GLYHMYGVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDS 180
           GLYHMYGV  VVGIE GY+GFYARNTIPLTPK VNNIHKRGGTILGTSRGGHNTTKIVDS
Sbjct: 156 GLYHMYGVHKVVGIEGGYRGFYARNTIPLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDS 215

Query: 181 IQHRGINQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTA 240
           IQHRGINQ                  EI+RRGLKVAVAGIPKTIDNDIPVIDKSFGFDTA
Sbjct: 216 IQHRGINQVYILGGDGTQKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTA 275

Query: 241 VEEAQRAISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEG 300
           VEEAQRAISAAHVESESN+N IGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEG
Sbjct: 276 VEEAQRAISAAHVESESNENGIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEG 335

Query: 301 EGGLFEYIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQI 360
           EGGLFEYIEHCI+E GHMVIVIAEGAGQEL+ +S+HKLD+QDASGNKLHQDVGLWISQQI
Sbjct: 336 EGGLFEYIEHCIKEHGHMVIVIAEGAGQELMSESIHKLDQQDASGNKLHQDVGLWISQQI 395

Query: 361 KDYFKKKKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVN 420
           KD+FK+K+K+ INLKYIDPTYMIRAVPSIASDNIFCTLLA SAIHGAMAGYTGFTVGPVN
Sbjct: 396 KDFFKEKRKMVINLKYIDPTYMIRAVPSIASDNIFCTLLAQSAIHGAMAGYTGFTVGPVN 455

Query: 421 GRHAYIPFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKN 448
           GRHAYIPF+RIIERQNKVVITDRMWARLLSSTNQPSFLHPKH+  EQ N KN
Sbjct: 456 GRHAYIPFHRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHVSDEQ-NDKN 506

BLAST of Cp4.1LG02g14440 vs. ExPASy TrEMBL
Match: A0A6J1K8N0 (ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111492688 PE=3 SV=1)

HSP 1 Score: 913 bits (2359), Expect = 0.0
Identity = 449/471 (95.33%), Postives = 451/471 (95.75%), Query Frame = 0

Query: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60
           MACNGVNGDS+GNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA
Sbjct: 1   MACNGVNGDSIGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60

Query: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120
           QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY
Sbjct: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120

Query: 121 GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180
           GVRTVVGIE GYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI
Sbjct: 121 GVRTVVGIEGGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180

Query: 181 NQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240
           NQ                  EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR
Sbjct: 181 NQVYILGGDGTQKGAAAIFEEIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240

Query: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300
           AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE
Sbjct: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300

Query: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360
           YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK
Sbjct: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360

Query: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420
           KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI
Sbjct: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420

Query: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLNG 453
           PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHM GE+ENGKNPQLNG
Sbjct: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMGGEEENGKNPQLNG 471

BLAST of Cp4.1LG02g14440 vs. ExPASy TrEMBL
Match: A0A6J1GAG1 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111452403 PE=3 SV=1)

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 449/470 (95.53%), Postives = 450/470 (95.74%), Query Frame = 0

Query: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60
           MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA
Sbjct: 1   MACNGVNGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVA 60

Query: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120
           QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY
Sbjct: 61  QNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMY 120

Query: 121 GVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180
           GVRTVVGIE GYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI
Sbjct: 121 GVRTVVGIEGGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGI 180

Query: 181 NQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240
           NQ                  EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR
Sbjct: 181 NQVYILGGDGTQKGAAAIFEEIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQR 240

Query: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300
           AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE
Sbjct: 241 AISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFE 300

Query: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360
           YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK
Sbjct: 301 YIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKK 360

Query: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420
           KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI
Sbjct: 361 KKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYI 420

Query: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQLN 452
           PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHM GE+ENGKNPQLN
Sbjct: 421 PFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMGGEEENGKNPQLN 470

BLAST of Cp4.1LG02g14440 vs. ExPASy TrEMBL
Match: A0A1S3BH55 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103489982 PE=3 SV=1)

HSP 1 Score: 817 bits (2111), Expect = 3.06e-296
Identity = 411/472 (87.08%), Postives = 427/472 (90.47%), Query Frame = 0

Query: 1   MACNGVNG-----DSL-GNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVN 60
           M CNG NG     DSL G  +HYVLEDVPHLTDYLPDLP   NPLQD+PAYSAVKQYFVN
Sbjct: 36  MDCNGGNGRDALGDSLTGGDYHYVLEDVPHLTDYLPDLPANLNPLQDHPAYSAVKQYFVN 95

Query: 61  VDDAVAQNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVC 120
           VDDAVAQNVVV NNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVC
Sbjct: 96  VDDAVAQNVVVHNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVC 155

Query: 121 GLYHMYGVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDS 180
           GLYHMYGV  VVGIE GY+GFYARNTIPLTPK VNNIHKRGGTILGTSRGGHNTTKIVDS
Sbjct: 156 GLYHMYGVHKVVGIEGGYRGFYARNTIPLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDS 215

Query: 181 IQHRGINQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTA 240
           IQHRGINQ                  EI+RRGLKVAVAGIPKTIDNDIPVIDKSFGFDTA
Sbjct: 216 IQHRGINQVYILGGDGTQKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTA 275

Query: 241 VEEAQRAISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEG 300
           VEEAQRAISAAHVESESN+N IGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEG
Sbjct: 276 VEEAQRAISAAHVESESNENGIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEG 335

Query: 301 EGGLFEYIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQI 360
           EGGLFEYIEHCI+E GHMVIVIAEGAGQEL+ +S+HKLD+QDASGNKLHQDVGLWISQQI
Sbjct: 336 EGGLFEYIEHCIKEHGHMVIVIAEGAGQELMSESIHKLDQQDASGNKLHQDVGLWISQQI 395

Query: 361 KDYFKKKKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVN 420
           KD+FK+K+K+ INLKYIDPTYMIRAVPSIASDNIFCTLLA SAIHGAMAGYTGFTVGPVN
Sbjct: 396 KDFFKEKRKMVINLKYIDPTYMIRAVPSIASDNIFCTLLAQSAIHGAMAGYTGFTVGPVN 455

Query: 421 GRHAYIPFNRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKN 448
           GRHAYIPF+RIIERQNKVVITDRMWARLLSSTNQPSFLHPKH+  EQ N KN
Sbjct: 456 GRHAYIPFHRIIERQNKVVITDRMWARLLSSTNQPSFLHPKHVSDEQ-NDKN 506

BLAST of Cp4.1LG02g14440 vs. ExPASy TrEMBL
Match: A0A6J1DJI3 (ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC111021059 PE=3 SV=1)

HSP 1 Score: 812 bits (2098), Expect = 2.09e-294
Identity = 401/463 (86.61%), Postives = 418/463 (90.28%), Query Frame = 0

Query: 7   NGDSLGNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQ 66
           NG      F YVLEDVPHLTDYLPDLP YPNPLQDNPAYS VKQYFV+VDDAVAQ+VVV 
Sbjct: 44  NGRDAPGDFRYVLEDVPHLTDYLPDLPAYPNPLQDNPAYSVVKQYFVDVDDAVAQSVVVH 103

Query: 67  NNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVV 126
            N+PRGTHFRR GPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGV  VV
Sbjct: 104 KNTPRGTHFRRTGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVHKVV 163

Query: 127 GIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ---- 186
           GIE GYKGFYARNTI LTPK VNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ    
Sbjct: 164 GIEGGYKGFYARNTISLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQVYIL 223

Query: 187 --------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAH 246
                         EI+RRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAH
Sbjct: 224 GGDGTQKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAH 283

Query: 247 VESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCI 306
           VESESN+NCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHC+
Sbjct: 284 VESESNENCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCL 343

Query: 307 EEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITI 366
           +EQGHMVIVIAEGAGQELI KSMH +D QDASGNKLHQDVGLWISQ+IKDYFK+ +KI I
Sbjct: 344 KEQGHMVIVIAEGAGQELISKSMHNMDNQDASGNKLHQDVGLWISQRIKDYFKEMRKIAI 403

Query: 367 NLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRII 426
           +LKYIDPTYMIRAVPSIASDNIFC+LLA SAIHGAMAGYTGFTVGPVNGRHAYIPF+RII
Sbjct: 404 DLKYIDPTYMIRAVPSIASDNIFCSLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFHRII 463

Query: 427 ERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQENGKNPQL 451
           ERQNKVVITDRMWARLLSSTNQPSFLHPKH+ GEQ NG NP +
Sbjct: 464 ERQNKVVITDRMWARLLSSTNQPSFLHPKHVSGEQ-NGTNPHI 505

BLAST of Cp4.1LG02g14440 vs. ExPASy TrEMBL
Match: A0A0A0L7Y3 (ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1)

HSP 1 Score: 812 bits (2097), Expect = 3.99e-294
Identity = 405/460 (88.04%), Postives = 416/460 (90.43%), Query Frame = 0

Query: 5   GVNGDSL-GNGFHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNV 64
           G  GDSL G  FHYVLEDVPHLTDYLPDLP   NPLQDNPAYSA KQYFVNVDDAVAQNV
Sbjct: 44  GAPGDSLTGGDFHYVLEDVPHLTDYLPDLPANLNPLQDNPAYSAAKQYFVNVDDAVAQNV 103

Query: 65  VVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVR 124
           VV NNSPRGTHFRRAGPRQKV FESDEVHACIVTCGGLCPGLNTVIREIVCGLY MYGV 
Sbjct: 104 VVHNNSPRGTHFRRAGPRQKVLFESDEVHACIVTCGGLCPGLNTVIREIVCGLYRMYGVH 163

Query: 125 TVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ- 184
            VVGIE GYKGFYARNTIPLTPK VNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ 
Sbjct: 164 RVVGIEGGYKGFYARNTIPLTPKSVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQV 223

Query: 185 -----------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIS 244
                            EI+RRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIS
Sbjct: 224 YILGGDGTQKGAAAIFEEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAIS 283

Query: 245 AAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIE 304
           AAHVESESN+N IGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIE
Sbjct: 284 AAHVESESNENGIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIE 343

Query: 305 HCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKK 364
           HCI+E GHMVIVIAEGAGQEL+ +S+HKLD+QDASGNKLHQDVGLWISQQIKDYFK+K+K
Sbjct: 344 HCIKEHGHMVIVIAEGAGQELMSESIHKLDQQDASGNKLHQDVGLWISQQIKDYFKEKRK 403

Query: 365 ITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFN 424
           + INLKYIDPTYMIRAVPSIASDNIFCTLLA SAIHGAMAGYTGFTVGPVNGRHAYIPF 
Sbjct: 404 MVINLKYIDPTYMIRAVPSIASDNIFCTLLAQSAIHGAMAGYTGFTVGPVNGRHAYIPFQ 463

Query: 425 RIIERQNKVVITDRMWARLLSSTNQPSFLHPKHMDGEQEN 445
           RI ERQNKVVITDRMWARLLSSTNQPSFLHPKH+  EQ N
Sbjct: 464 RITERQNKVVITDRMWARLLSSTNQPSFLHPKHVSDEQNN 503

BLAST of Cp4.1LG02g14440 vs. TAIR 10
Match: AT4G26270.1 (phosphofructokinase 3 )

HSP 1 Score: 676.4 bits (1744), Expect = 1.6e-194
Identity = 335/446 (75.11%), Postives = 377/446 (84.53%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           YVLEDVPHL+DYLP LP YPNPLQDNPAYS VKQYFV+ DD+V Q +VV  + PRG HFR
Sbjct: 18  YVLEDVPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKIVVHKDGPRGIHFR 77

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPRQKV+FESDEVHACIVTCGGLCPGLNTVIREIV  L +MYGV+ ++GI+ GY+GFY
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           A+NT+ L  K+VN+IHKRGGTILGTSRGGH+TTKIVDSIQ RGINQ              
Sbjct: 138 AKNTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGAS 197

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               EI+RRGLKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHVE+ES +N I
Sbjct: 198 VIFEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGI 257

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           G+VKLMGR SGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIE  ++E GHMV+VI
Sbjct: 258 GVVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVI 317

Query: 317 AEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTYM 376
           AEGAGQ+L+ KSM  +  +DASGNKL +DVGLW+SQ IKD+F +KK + +NLKYIDPTYM
Sbjct: 318 AEGAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHFNQKKMV-MNLKYIDPTYM 377

Query: 377 IRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVITD 436
           IRAVPS ASDN++CTLLA SA+HGAMAGYTG+  G VNGR  YIPF RI E+QN VVITD
Sbjct: 378 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITD 437

Query: 437 RMWARLLSSTNQPSFLHPKHMDGEQE 445
           RMWARLLSSTNQPSFL PK +   +E
Sbjct: 438 RMWARLLSSTNQPSFLGPKDVFDNKE 462

BLAST of Cp4.1LG02g14440 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 672.9 bits (1735), Expect = 1.8e-193
Identity = 332/446 (74.44%), Postives = 374/446 (83.86%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           Y+L+DVPHL DYLPDLP YPNPLQDNPAYS VKQYFV+ DD+V + VVV  + PRG HFR
Sbjct: 18  YILQDVPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKVVVHKDGPRGVHFR 77

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPRQKV+FESDEVHACIVTCGGLCPGLNTVIRE+V  L +MYGV+ ++GI+ GY+GFY
Sbjct: 78  RAGPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFY 137

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           A+NTIPL  K+VN+IHKRGGTI+GTSRGGH+T KIVDSIQ RGINQ              
Sbjct: 138 AKNTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGAS 197

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               EI+RR LKVAV GIPKTIDNDIPVIDKSFGFDTAVEEAQRAI+AAHVE+ESN+N I
Sbjct: 198 VIFEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGI 257

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           G VKLMGR SG+IAMYATLASRDVDCCLIPESPFYLEGEGGLFE+IE  +++ GHMVIV+
Sbjct: 258 GFVKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVL 317

Query: 317 AEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTYM 376
           AEGAGQ+L+ KSM      DASGNKL +DVGLW+SQ IKD+FKK K + +NLKYIDPTYM
Sbjct: 318 AEGAGQDLMCKSMES-TPMDASGNKLLKDVGLWLSQSIKDHFKKNKMV-MNLKYIDPTYM 377

Query: 377 IRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVITD 436
           IRAVPS ASDN++CTLLA SA+HGAMAGYTG+T G VNGR  YIPF RI E QN VVITD
Sbjct: 378 IRAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITD 437

Query: 437 RMWARLLSSTNQPSFLHPKHMDGEQE 445
           RMWARLLSSTNQPSFL PK    E++
Sbjct: 438 RMWARLLSSTNQPSFLGPKDTSEEKK 461

BLAST of Cp4.1LG02g14440 vs. TAIR 10
Match: AT4G29220.1 (phosphofructokinase 1 )

HSP 1 Score: 652.1 bits (1681), Expect = 3.2e-187
Identity = 325/437 (74.37%), Postives = 368/437 (84.21%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           Y+LEDVPH +D  PD P YPNPLQDN AYS VKQYFV+ DD V Q +VV  +SPRGTHFR
Sbjct: 19  YILEDVPHFSDDFPDHPTYPNPLQDNAAYSVVKQYFVDEDDTVPQKIVVHPDSPRGTHFR 78

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPRQ+V+FESD+V ACIVTCGGLCPGLNTVIREIVCGL +MYGV+ ++GI+ GY+GFY
Sbjct: 79  RAGPRQRVYFESDDVLACIVTCGGLCPGLNTVIREIVCGLSYMYGVKRILGIDGGYRGFY 138

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           ARNTI L  K VN+IH+ GGTILGTSRGGHNTTKIVDSIQ RGINQ              
Sbjct: 139 ARNTIHLDLKTVNDIHRSGGTILGTSRGGHNTTKIVDSIQDRGINQVYIIGGDGSQKGAA 198

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               EI++R LKVAVAGIPKTIDNDIP+ID+SFGFDTAVEEAQRAI+AAHVE+ S +N I
Sbjct: 199 AIFEEIRKRKLKVAVAGIPKTIDNDIPIIDRSFGFDTAVEEAQRAINAAHVEATSFENGI 258

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           GLVKLMGR SGFIAM+ATLASRDVDCCLIPESPF+LEG GGLFE+I+  ++E GHMVIVI
Sbjct: 259 GLVKLMGRYSGFIAMHATLASRDVDCCLIPESPFFLEGSGGLFEFIDKRLKESGHMVIVI 318

Query: 317 AEGAGQELILKSMHKLDE-QDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTY 376
           AEGAGQ+L+ +SM +    +DASGNKL QD+GLWISQ+IKD+F   KK+T+ LKYIDPTY
Sbjct: 319 AEGAGQDLLSESMKESTTLKDASGNKLLQDIGLWISQRIKDHF--AKKMTLTLKYIDPTY 378

Query: 377 MIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVIT 435
           MIRAVPS ASDN+ CTLLA SA+HG MAGY GFTVG VNGRH YIPFNRI E+QNKVVIT
Sbjct: 379 MIRAVPSNASDNVCCTLLAQSAVHGVMAGYNGFTVGLVNGRHTYIPFNRITEKQNKVVIT 438

BLAST of Cp4.1LG02g14440 vs. TAIR 10
Match: AT4G32840.1 (phosphofructokinase 6 )

HSP 1 Score: 651.7 bits (1680), Expect = 4.2e-187
Identity = 324/456 (71.05%), Postives = 377/456 (82.68%), Query Frame = 0

Query: 1   MACNGVNGD-SLGNG-FHYVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDA 60
           MA NGV+    L  G   YVLEDVPHL+DY+ DLP YPNPLQ N AYS V+QYFV+ DD 
Sbjct: 1   MASNGVDEQIKLVEGPAGYVLEDVPHLSDYILDLPTYPNPLQSNAAYSVVRQYFVDEDDT 60

Query: 61  VAQNVVVQNNSPRGTHFRRAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYH 120
           V + +VV  +SPRGTHFRRAGPRQKV+F+  +V ACIVTCGGLCPGLNTVIREIVCGL+ 
Sbjct: 61  VQEKIVVHKDSPRGTHFRRAGPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHF 120

Query: 121 MYGVRTVVGIEAGYKGFYARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHR 180
           MYGV  V+G++ G++GFY++NT+ LTPK V++IHKRGGTILGTSRGGH+T+KIVD+IQ R
Sbjct: 121 MYGVTEVIGVDCGFRGFYSKNTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDR 180

Query: 181 GINQ------------------EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEA 240
            INQ                  EI+RRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEA
Sbjct: 181 EINQVYIIGGDGTQKGANAIYKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEA 240

Query: 241 QRAISAAHVESESNDNCIGLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGL 300
           QRAI+AAHVE+ S +N IG+VKLMGR SGFIAMYATLASRDVDCCLIPESPFYLEG+GGL
Sbjct: 241 QRAINAAHVEATSVENGIGIVKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGL 300

Query: 301 FEYIEHCIEEQGHMVIVIAEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYF 360
           +E+I   + E GHMVIVIAEGAGQ+L+ +S   +++QDASGNKL +DVGLW+S +IK+YF
Sbjct: 301 YEFIAKRLRENGHMVIVIAEGAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYF 360

Query: 361 KKKKKITINLKYIDPTYMIRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHA 420
            K   + I LKYIDPTYMIRA+P+ ASDN++ TLLA SA+HGAMAGYTGF  G VNGRH 
Sbjct: 361 AKHNVMDITLKYIDPTYMIRAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHT 420

Query: 421 YIPFNRIIERQNKVVITDRMWARLLSSTNQPSFLHP 437
           YIPFNRI ERQNKVVITDRMWAR+LSSTNQPSF++P
Sbjct: 421 YIPFNRITERQNKVVITDRMWARMLSSTNQPSFMNP 453

BLAST of Cp4.1LG02g14440 vs. TAIR 10
Match: AT5G61580.1 (phosphofructokinase 4 )

HSP 1 Score: 630.9 bits (1626), Expect = 7.7e-181
Identity = 303/436 (69.50%), Postives = 365/436 (83.72%), Query Frame = 0

Query: 17  YVLEDVPHLTDYLPDLPGYPNPLQDNPAYSAVKQYFVNVDDAVAQNVVVQNNSPRGTHFR 76
           +VLEDVPHLT +LPDLP YPNPL+++ AY+ VK+ FV+ +D VAQN+VVQ  S RG HFR
Sbjct: 69  FVLEDVPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNIVVQKGSKRGVHFR 128

Query: 77  RAGPRQKVFFESDEVHACIVTCGGLCPGLNTVIREIVCGLYHMYGVRTVVGIEAGYKGFY 136
           RAGPR++V+F SDEV ACIVTCGGLCPG+NTVIREIVCGL +MYGV  ++GI+ GY+GFY
Sbjct: 129 RAGPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFY 188

Query: 137 ARNTIPLTPKMVNNIHKRGGTILGTSRGGHNTTKIVDSIQHRGINQ-------------- 196
           ++NT+ LTPK+VN+IHKRGGT L TSRGGH+T KIVD+IQ RGINQ              
Sbjct: 189 SKNTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAE 248

Query: 197 ----EIKRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAISAAHVESESNDNCI 256
               E++RRGL+VAV+GIPKTIDNDI VIDKSFGFDTAVEEAQRAI+AAHVE ES +N +
Sbjct: 249 KIYEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGV 308

Query: 257 GLVKLMGRNSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEHCIEEQGHMVIVI 316
           G+VKLMGR SGFIAM ATLA+RDVDCCLIPESPF+LEG+GGLFE+IE  ++E  HMVIVI
Sbjct: 309 GIVKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVI 368

Query: 317 AEGAGQELILKSMHKLDEQDASGNKLHQDVGLWISQQIKDYFKKKKKITINLKYIDPTYM 376
           AEGAGQ+ + +SM   + +DASGN+L  DVGLW++QQIKD+F   +K+ IN+KYIDPTYM
Sbjct: 369 AEGAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRKMMINMKYIDPTYM 428

Query: 377 IRAVPSIASDNIFCTLLAHSAIHGAMAGYTGFTVGPVNGRHAYIPFNRIIERQNKVVITD 435
           IRA+PS ASDN++CTLLA SA+HGAMAGY+GFTVGPVN RHAYIP +++ E  N V +TD
Sbjct: 429 IRAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTD 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94AA42.2e-19375.11ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE... [more]
Q9C5J72.5e-19274.44ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE... [more]
Q9M0F94.5e-18674.37ATP-dependent 6-phosphofructokinase 1 OS=Arabidopsis thaliana OX=3702 GN=PFK1 PE... [more]
Q9M0765.9e-18671.05ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE... [more]
Q9FKG31.1e-17969.50ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
XP_023524893.10.096.18ATP-dependent 6-phosphofructokinase 3-like [Cucurbita pepo subsp. pepo][more]
KAG6606740.10.095.75ATP-dependent 6-phosphofructokinase 3, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022997851.10.095.33ATP-dependent 6-phosphofructokinase 3-like [Cucurbita maxima][more]
XP_022948872.10.095.53ATP-dependent 6-phosphofructokinase 3-like [Cucurbita moschata][more]
XP_008447566.16.31e-29687.08PREDICTED: ATP-dependent 6-phosphofructokinase 6-like [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1K8N00.095.33ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111492688 ... [more]
A0A6J1GAG10.095.53ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11145240... [more]
A0A1S3BH553.06e-29687.08ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103489982 PE=3... [more]
A0A6J1DJI32.09e-29486.61ATP-dependent 6-phosphofructokinase OS=Momordica charantia OX=3673 GN=LOC1110210... [more]
A0A0A0L7Y33.99e-29488.04ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G26270.11.6e-19475.11phosphofructokinase 3 [more]
AT5G56630.11.8e-19374.44phosphofructokinase 7 [more]
AT4G29220.13.2e-18774.37phosphofructokinase 1 [more]
AT4G32840.14.2e-18771.05phosphofructokinase 6 [more]
AT5G61580.17.7e-18169.50phosphofructokinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 290..302
score: 36.69
coord: 95..114
score: 42.31
coord: 233..249
score: 29.41
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 94..381
e-value: 5.9E-49
score: 166.9
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typePIRSFPIRSF000534ATP_PFK_TP0108coord: 177..438
e-value: 2.3E-129
score: 429.7
coord: 18..182
e-value: 1.1E-66
score: 223.1
NoneNo IPR availableGENE3D3.40.50.450coord: 87..182
e-value: 5.9E-30
score: 106.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 432..453
NoneNo IPR availablePANTHERPTHR45770FAMILY NOT NAMEDcoord: 16..445
NoneNo IPR availablePANTHERPTHR45770:SF17ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASEcoord: 16..445
IPR035966Phosphofructokinase superfamilyGENE3D3.40.50.460Phosphofructokinase domaincoord: 213..370
e-value: 1.9E-47
score: 163.4
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 25..439

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g14440.1Cp4.1LG02g14440.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding