Cp4.1LG02g13820 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g13820
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	delta(7)-sterol-C5(6)-desaturase-like
Location	Cp4.1LG02: 12298375 .. 12302129 (+)
RNA-Seq Expression	Cp4.1LG02g13820
Synteny	Cp4.1LG02g13820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCTTTCTTTCATCAATCTAAGCAGCACAGCCACTTTTTCGGTTATAAATTCTCACACCCTTTTCGATTCCAACTCAGTCTCTATCTATCTTCCTCTGCCTTTGATTTCACTGTTTTTCCTCTGGTTTTTCCACTCGCCGGAGTTGGGCTGATTCCGACGGCCATGGGCGGCAGACCGCCATATTTGGAGCTCTTTCTGGAGGAGACTAGTTTGTACAACCGCATTGTTTTGGGAACCATTTTTCCTGACTTTGTTTTGGACCCATTTCCTCATATTCTTCAAACATGGCTTAGAAACTGCATTGGTGGATTCCTAATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTTCATCTATTACTGGAAGCGAAATGTCTATGTTCCTAAAGGTCTTTGTCTTTATTGTTCCTATATCTCAACTGCCTCATTAATGAGTTCTAATTATGAGTTCTAATTCTAGTCATTTGGGTTCTCTTATTTCATTTTGTTCTTCGTTTCAGCAATGAATTCTGTCATTGGAGAAAATCTGGATATGTATTTCTTTTAATTTCTGTCACTGTTCTTCAGATTCATGGACCTGAATCCTTCAACTCTGGATATTGCATGCTATATTTGATTCATTTTGTGCTACTGCAAGGCTTTGATTCAGATCAAATTGACATATCTTTGATGGGGCTGTTTTTTTCCCTTCTGAGATGCTAGTGGTTCTGGTCTTTTCCATGATTATGATTTATTTGTGCAAGTTTTTGTTCTAAGAAGAACTGTAATAGCCATTAGAACTGCTACTTAAAGAGGGTAGTTTAGCTCTACAAATGGATGATTCTGCTACTCATGATGAACATATATGTCTAACCAAAAGTTATGTGTCACAGCTCAATGCTTGTATGATTGTTTGACCTGTTGGACACTTGATTGCAGATTCTATTCCATCAAATAGATTGATGCTATTGCAGATTCATGCAACAGTCAAGGCCATGCCATTGTACTGTGTCCTTCCGACGTTTTCTGAATACGTGGTAGAACACGATTGGACGAGATGCTATCCGAGGGTATTCGATGTGGGATGGCTTGCCTATGCCTTCCATGTAACTACTTATCTTACGTTTGTAGAGTTTTCTATTTATTGGATGCATAGGGCACTCCATGATGTAAAGCCTCTTTACAAGTATCTTCATGCTAAGCATCATATGTACAACAAGAAAGCTACTCTCTCACCCTTTGCTGGTAACTTCTTAACTTACTATCCCAATTTTTGCAACATCTAATTCATGTCAAATGAACTTCTTTGATGTCATTCTTGTTTGATTTCCTTAAGATGTGTTAAACGAAGCTGAAATACAAACCGATCTCGATACGGAACTGTTTCCCAAAAGATCTCTTACAAAAAAACTAACCCATACTAACAGAAGGAAAATATATGCTTAGCATTAAGTTGCTGTTGTTAACTGTTAGGACAGTAGTAGGTATTGAATTTAGAACTCTTGTGTAACCGCCTAAGCTTATCACTAGTAGATATAGTTCATTTTGGACTTTTCAAAACGCGTCTGCTAGGGAGAAGTTTCCACATCCTTATAAGGAATGCTTCGTTTCCCTCTCCAACCAATGTGGGATCTCACCATCCACCTCCTTTAGGGGCTCAACGTCCTCGCTAGCACACCACCCGGTAACTTTCTCACCATCCACCTCCCTTGGGGGCCATCCAGTAACTTTCTCTGATACCATTTGCAACAGCCCAAGTCTATCACTAGCAGNCCTTGGGGGCCATCCAGTAACTTTCTCTGATACCATTTGCAACCGCCCAAGTCTATCACCAGCAAATATTGTCCTCTTTGGGTTTTCCCTTCTGGGTTTCGGTTTTAAAACGTGTTTGCTAGGGAGAGGTTTCCACACCCTTGTAAGGAATGTTTCGTTTCCCTCTCCCGACCAATGTGAGATCTCACATTTTGTTTCCTTAAAACCACATGAGTTTTTGAAAAATGCCAATTGAAAACGATATTGATCTATCGTGAACATCAGGAGTGGCTACTCGCCTCCATAGGACTTACTCGAGTCACACGAACTTGACTTTTGATGTGCTCTTCTTATATCATTTTGGCATTTATCTTAGCAGAAAACTCTTCCTTATATCGACTAAATAGAGCTCGTTTTAGCGACAAAGACTGCAAGTTTTATATCGAATGCATTGCTTCATTTTGACAGAAACTTCAGAATCTGTGCAGCTTGAGTTGATTAGAGAAATATAGATATTGTTCTCTTAGAATGAAAAAGTTCTTTGGTTGTTTCTCATTAATGGAAAATTATGTGATAAATGGTCTGTTTGTATGACAGGACTAGCATTTCACCCAGTGGATGGGATATTGCAGGCAATACCTCACTTGTTCGCTCTGTTTCTCATTCCAATCCATTTTAGAACACATATCATGCTGCTGTTTTTTGAGGTGGTATGGACAACAAACATTCACGATTGCATCCACGCACAAATGTGGCCAGTCATGGGTGCTGGCTACCACACCCTTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTCGGGACACTTCTTGACCCAATGGATACACAACACAAGTTGTCGTGATCGGTTGCTTCAACACATTTATAGCTTTTGTTGATTCTTTGTGTTCAATGTAAATCAAGTTGGTCATGTCTGTACACCATCGTCAAATTTAACATCTTACGAATTACCTTGACAGCCAAATGTAGTAAAGCCAGACAGTTGCCCCATACAATTAGTCGAGATGATAGCTTCATCGAACAAGTGTTTCAGGCGTTGTACCTGTTTCTACATTGCAAGAGAGAAACGAAGCACTACCAAAAGCAAGTTGGAATGAGAGACATTGACAACTTGCTTTTTCTTTTACTTTTTATCCCATTTCAATATGCATCTCCTTACTTTTCAAAGCAATAGGCAATGTCCATAACAGTCTAGAGCTGCCATTGTTCATCCTTCAATAATAAAAGGTATTCATTCACTTTGATTGCAACAAAAATAAGTCGACCCCACCTCCCAAAACATCTCAACTACGAAAGGAAATGCTTAAAGTTAGCAACAGGGAATTGAACTGTTCATCATGGTACATCAACATTTCAGCCTCAAGATCAGCAAAAACTGCAAAGCTTTTGCTCTTATCTAGTTTCTGTTTGTACAAAAGGAATATAAATGAAAAGAAAATACATAAAGGTGAAAAAGGGAAAACAAAAACAGAATCACACACAATTGGTACATTGCTGGTAGAATGGATGCATCAATGTAGAAGGAAGGGGACACAGTTTATAGCTTATTTTGAGATGAATTTCAATGTAATTGATTACCAAACAAGCCCCTTTCGTTTCCTAAACCTATCAACACGATCGGTGCTCATTCGAGATTTCTCAGGCTTGTTTACTTTTTTGGACTTCTCAAGCCGACTATCTAAACTGTTCCGGGAAACTTCTGGAAGATCAGCAGCCAATTCGAACGTATCTGAAGGCCCAGTTTCGGATGGTTTTTTCTGACCGTCGATTTCTACTTCAGCTTTCTCAGAAGCATTTGTTGATGCTTTTGATTTTCCCAGTGACTGTACAAATTTCTTTAAATGTTTGATGAACTCAGGGTAGAGCTCAAGATTGCAGTGCCCACCTCCACTTACCCATAGAGGTTCATATTTTTGGTTGCAAAGATCCCAGAGCTGCTTTCCATGGGACCAATCAACAACTTCATCAGCTGTCCCCT

mRNA sequence

ATCTTTCTTTCATCAATCTAAGCAGCACAGCCACTTTTTCGGTTATAAATTCTCACACCCTTTTCGATTCCAACTCAGTCTCTATCTATCTTCCTCTGCCTTTGATTTCACTGTTTTTCCTCTGGTTTTTCCACTCGCCGGAGTTGGGCTGATTCCGACGGCCATGGGCGGCAGACCGCCATATTTGGAGCTCTTTCTGGAGGAGACTAGTTTGTACAACCGCATTGTTTTGGGAACCATTTTTCCTGACTTTGTTTTGGACCCATTTCCTCATATTCTTCAAACATGGCTTAGAAACTGCATTGGTGGATTCCTAATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTTCATCTATTACTGGAAGCGAAATGTCTATGTTCCTAAAGATTCTATTCCATCAAATAGATTGATGCTATTGCAGATTCATGCAACAGTCAAGGCCATGCCATTGTACTGTGTCCTTCCGACGTTTTCTGAATACGTGGTAGAACACGATTGGACGAGATGCTATCCGAGGGTATTCGATGTGGGATGGCTTGCCTATGCCTTCCATGTAACTACTTATCTTACGTTTGTAGAGTTTTCTATTTATTGGATGCATAGGGCACTCCATGATGTAAAGCCTCTTTACAAGTATCTTCATGCTAAGCATCATATGTACAACAAGAAAGCTACTCTCTCACCCTTTGCTGGACTAGCATTTCACCCAGTGGATGGGATATTGCAGGCAATACCTCACTTGTTCGCTCTGTTTCTCATTCCAATCCATTTTAGAACACATATCATGCTGCTGTTTTTTGAGGTGGTATGGACAACAAACATTCACGATTGCATCCACGCACAAATGTGGCCAGTCATGGGTGCTGGCTACCACACCCTTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTCGGGACACTTCTTGACCCAATGGATACACAACACAAGTTGTCGTGATCGGTTGCTTCAACACATTTATAGCTTTTGTTGATTCTTTGTGTTCAATGTAAATCAAGTTGGTCATGTCTGTACACCATCGTCAAATTTAACATCTTACGAATTACCTTGACAGCCAAATGTAGTAAAGCCAGACAGTTGCCCCATACAATTAGTCGAGATGATAGCTTCATCGAACAAGTGTTTCAGGCGTTGTACCTGTTTCTACATTGCAAGAGAGAAACGAAGCACTACCAAAAGCAAGTTGGAATGAGAGACATTGACAACTTGCTTTTTCTTTTACTTTTTATCCCATTTCAATATGCATCTCCTTACTTTTCAAAGCAATAGGCAATGTCCATAACAGTCTAGAGCTGCCATTGTTCATCCTTCAATAATAAAAGGTATTCATTCACTTTGATTGCAACAAAAATAAGTCGACCCCACCTCCCAAAACATCTCAACTACGAAAGGAAATGCTTAAAGTTAGCAACAGGGAATTGAACTGTTCATCATGGTACATCAACATTTCAGCCTCAAGATCAGCAAAAACTGCAAAGCTTTTGCTCTTATCTAGTTTCTGTTTGTACAAAAGGAATATAAATGAAAAGAAAATACATAAAGGTGAAAAAGGGAAAACAAAAACAGAATCACACACAATTGGTACATTGCTGGTAGAATGGATGCATCAATGTAGAAGGAAGGGGACACAGTTTATAGCTTATTTTGAGATGAATTTCAATGTAATTGATTACCAAACAAGCCCCTTTCGTTTCCTAAACCTATCAACACGATCGGTGCTCATTCGAGATTTCTCAGGCTTGTTTACTTTTTTGGACTTCTCAAGCCGACTATCTAAACTGTTCCGGGAAACTTCTGGAAGATCAGCAGCCAATTCGAACGTATCTGAAGGCCCAGTTTCGGATGGTTTTTTCTGACCGTCGATTTCTACTTCAGCTTTCTCAGAAGCATTTGTTGATGCTTTTGATTTTCCCAGTGACTGTACAAATTTCTTTAAATGTTTGATGAACTCAGGGTAGAGCTCAAGATTGCAGTGCCCACCTCCACTTACCCATAGAGGTTCATATTTTTGGTTGCAAAGATCCCAGAGCTGCTTTCCATGGGACCAATCAACAACTTCATCAGCTGTCCCCT

Coding sequence (CDS)

ATGGGCGGCAGACCGCCATATTTGGAGCTCTTTCTGGAGGAGACTAGTTTGTACAACCGCATTGTTTTGGGAACCATTTTTCCTGACTTTGTTTTGGACCCATTTCCTCATATTCTTCAAACATGGCTTAGAAACTGCATTGGTGGATTCCTAATTTACTTCTTCTCTGGCTTTCTTTGGTGCTTCTTCATCTATTACTGGAAGCGAAATGTCTATGTTCCTAAAGATTCTATTCCATCAAATAGATTGATGCTATTGCAGATTCATGCAACAGTCAAGGCCATGCCATTGTACTGTGTCCTTCCGACGTTTTCTGAATACGTGGTAGAACACGATTGGACGAGATGCTATCCGAGGGTATTCGATGTGGGATGGCTTGCCTATGCCTTCCATGTAACTACTTATCTTACGTTTGTAGAGTTTTCTATTTATTGGATGCATAGGGCACTCCATGATGTAAAGCCTCTTTACAAGTATCTTCATGCTAAGCATCATATGTACAACAAGAAAGCTACTCTCTCACCCTTTGCTGGACTAGCATTTCACCCAGTGGATGGGATATTGCAGGCAATACCTCACTTGTTCGCTCTGTTTCTCATTCCAATCCATTTTAGAACACATATCATGCTGCTGTTTTTTGAGGTGGTATGGACAACAAACATTCACGATTGCATCCACGCACAAATGTGGCCAGTCATGGGTGCTGGCTACCACACCCTTCATCATACAAGATACCGCTACAATTATGGCCATTACTCCATATGGATGGATTGGATGTTCGGGACACTTCTTGACCCAATGGATACACAACACAAGTTGTCGTGA

Protein sequence

MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS

Homology

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BLAST of Cp4.1LG02g13820 vs. ExPASy Swiss-Prot
Match: Q9ZT29 (Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.5e-112
Identity = 177/266 (66.54%), Postives = 216/266 (81.20%), Query Frame = 0


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL+ F+EETS YNR+VLGT  P+    P PH+LQ WLRN IGG L+YF SGFLWCF+IY+
Sbjct: 4   YLKQFVEETSFYNRLVLGTFMPESWWGPLPHMLQGWLRNYIGGVLLYFISGFLWCFYIYH 63

Query: 67  WKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            KRNVY+PKD+IPSN+ MLLQI   +KAMP YC LP+ SEY++E+ WT+C+ R+ DVGWL
Sbjct: 64  LKRNVYIPKDAIPSNKAMLLQISVAMKAMPWYCALPSLSEYMIENGWTKCFARISDVGWL 123

Query: 127 AYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
           +Y  +   YL  VEF IYWMH  LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHP+DG
Sbjct: 124 SYVIYAAIYLVIVEFGIYWMHMELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ AL L+P+HF THI L+F E +WT NIHDCIH +++PVMGAGYHT+HH  YR
Sbjct: 184 ILQAVPHVVALLLVPMHFSTHIALIFLEALWTANIHDCIHGKVFPVMGAGYHTIHHRTYR 243

Query: 247 YNYGHYSIWMDWMFGTLLDPMDTQHK 273
           +NYGHY+IWMDWMFGTL DP++   K
Sbjct: 244 HNYGHYTIWMDWMFGTLRDPVEEDAK 269

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy Swiss-Prot
Match: Q39208 (Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 SV=2)

HSP 1 Score: 387.9 bits (995), Expect = 9.6e-107
Identity = 169/261 (64.75%), Postives = 208/261 (79.69%), Query Frame = 0


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL  F++ETS YNRIVL  + P  + +P PH LQTWLRN + G L+YF SGFLWCF+IYY
Sbjct: 7   YLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYY 66

Query: 67  WKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVY+PKD+IP+ + M LQ+   +KAMP Y +LPT SE ++E  WT+C+  + + GW+
Sbjct: 67  LKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWI 126

Query: 127 AYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
            Y  ++  YL FVEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHPVDG
Sbjct: 127 LYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPVDG 186

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALF++PIHF THI LLF E +WT NIHDCIH  +WPVMGAGYHT+HHT Y+
Sbjct: 187 ILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTIHHTTYK 246

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L DP+
Sbjct: 247 HNYGHYTIWMDWMFGSLRDPL 267

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy Swiss-Prot
Match: Q9M883 (Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=HDF7 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 2.3e-100
Identity = 159/261 (60.92%), Postives = 202/261 (77.39%), Query Frame = 0


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           Y +  + ETS YNR+VL  + P  + +P PH LQTWLRN + G ++YF SGFLWCF+IYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVYVPK+SIP+ + MLLQI+  +KAMP Y +LP  SEY++EH WT+CY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
               ++  YL  VEF IYW+H+ LHD+K LYK+LHA HHMYNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQAIPH+ ALF++PIH  TH+ LLF E +WT +IHDCIH  +WP+MGAGYHT+HHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy Swiss-Prot
Match: Q9EQS5 (Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 5.1e-23
Identity = 75/233 (32.19%), Postives = 124/233 (53.22%), Query Frame = 0


Query: 47  IGGFLIYFFSGFLWCFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSE 106
           +G +++YFF   L  +F+Y    +  +       N++   +I  TVK++P +  +PT S 
Sbjct: 40  LGAYILYFFCATLSYYFVY---DHSLMKHPQFLKNQVS-REIMFTVKSLP-WISIPTVSL 99

Query: 107 YVVE-HDWTRCYPRV--FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAK 166
           +++E   +++ Y  +  F  GW+     V ++L F +  IYW+HR LH  + LYK++H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRGLHH-RLLYKHIHKP 159

Query: 167 HHMYNKKATLSPFAGLAFHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHD 226
           HH++      +PFA  AFHPVDG LQ++P+    F+ P+H   ++ L     VWT +IHD
Sbjct: 160 HHIWK---IPTPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 ---CIHAQMWP-VMGAGYHTLHHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
               +     P + G+ +HT HH  + YNYG Y    D + G+   P   + K
Sbjct: 220 GDFRVPQIFRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGK 263

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy Swiss-Prot
Match: O88822 (Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 3.3e-22
Identity = 74/233 (31.76%), Postives = 124/233 (53.22%), Query Frame = 0


Query: 47  IGGFLIYFFSGFLWCFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSE 106
           +G +++YFF   L  +F+Y    +  +       N++   +I  TVK++P +  +PT S 
Sbjct: 40  LGAYILYFFCATLSYYFVY---DHSLMKHPQFLKNQVS-REIVFTVKSLP-WISIPTVSL 99

Query: 107 YVVE-HDWTRCYPRV--FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAK 166
           +++E   +++ Y  +  F  GW+     V ++L F +  IYW+HR LH  + +YK +H  
Sbjct: 100 FLLELRGYSKLYDDIGDFPNGWIHLMVSVVSFLFFTDMLIYWIHRGLHH-RLVYKRIHKP 159

Query: 167 HHMYNKKATLSPFAGLAFHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHD 226
           HH++      +PFA  AFHPVDG LQ++P+    F+ P+H   ++ L     VWT +IHD
Sbjct: 160 HHIWK---IPTPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHD 219

Query: 227 ---CIHAQMWP-VMGAGYHTLHHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
               +   + P + G+ +HT HH  + YNYG Y    D + G+   P   + K
Sbjct: 220 GDFRVPQILRPFINGSAHHTDHHMFFDYNYGQYFTLWDRIGGSFKHPSSFEGK 263

[Close]

BLAST of Cp4.1LG02g13820 vs. NCBI nr
Match: XP_023525270.1 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 596 bits (1537), Expect = 9.53e-216
Identity = 274/274 (100.00%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
           FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL
Sbjct: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. NCBI nr
Match: KAG6607054.1 (hypothetical protein SDJN03_00396, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 595 bits (1534), Expect = 2.73e-215
Identity = 273/274 (99.64%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CFFIYYWKRNVYVPKDSIPSN+LMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
           FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL
Sbjct: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. NCBI nr
Match: XP_022948870.1 (delta(7)-sterol-C5(6)-desaturase-like [Cucurbita moschata] >KAG7036755.1 hypothetical protein SDJN02_00375, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 595 bits (1533), Expect = 3.88e-215
Identity = 272/274 (99.27%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CFFIYYWKRNVYVPKDSIPSN+LMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
           FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHPVDGILQAIPHLFALFL+PIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL
Sbjct: 181 FHPVDGILQAIPHLFALFLVPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. NCBI nr
Match: XP_022998219.1 (delta(7)-sterol-C5(6)-desaturase-like [Cucurbita maxima])

HSP 1 Score: 592 bits (1527), Expect = 3.19e-214
Identity = 271/274 (98.91%), Postives = 273/274 (99.64%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CFFIYYWKRNVYVPKDSIPSN+LMLLQIHAT+KAMPLYCVLPTFSEYVVEHDWTRCYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
           FDVGWLAYAFHVTTYLTFVEF IYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL
Sbjct: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. NCBI nr
Match: XP_038894105.1 (delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894107.1 delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida])

HSP 1 Score: 539 bits (1388), Expect = 5.00e-193
Identity = 238/274 (86.86%), Postives = 256/274 (93.43%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGG PPYLELF+EET  YNRIVLGTIFP F LDPFPHI+QTWLRNCIGGFLIYFFS FLW
Sbjct: 1   MGGGPPYLELFVEETDFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGFLIYFFSAFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CF+IYYWKRNVY+PKDSIPSN+ MLLQI  T+KAMPLYC+LPTFSEYVVEHDWT+CYPRV
Sbjct: 61  CFYIYYWKRNVYIPKDSIPSNKSMLLQIFVTIKAMPLYCILPTFSEYVVEHDWTKCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
            DVGW AYAFHV TYL F+EFSIYWMHR LHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHVITYLAFIEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHP+DGILQAIPHLFALFLIP HFRTHI+LLFFEV+WTTNIHDCIHA+MWPVMGAGYHT+
Sbjct: 181 FHPIDGILQAIPHLFALFLIPTHFRTHIVLLFFEVIWTTNIHDCIHARMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTL+DPMDT+ K S
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLIDPMDTEGKGS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy TrEMBL
Match: A0A6J1GB39 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111452401 PE=3 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 1.88e-215
Identity = 272/274 (99.27%), Postives = 274/274 (100.00%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CFFIYYWKRNVYVPKDSIPSN+LMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
           FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHPVDGILQAIPHLFALFL+PIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL
Sbjct: 181 FHPVDGILQAIPHLFALFLVPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy TrEMBL
Match: A0A6J1KDR9 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC111492934 PE=3 SV=1)

HSP 1 Score: 592 bits (1527), Expect = 1.55e-214
Identity = 271/274 (98.91%), Postives = 273/274 (99.64%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CFFIYYWKRNVYVPKDSIPSN+LMLLQIHAT+KAMPLYCVLPTFSEYVVEHDWTRCYPRV
Sbjct: 61  CFFIYYWKRNVYVPKDSIPSNKLMLLQIHATIKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
           FDVGWLAYAFHVTTYLTFVEF IYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA
Sbjct: 121 FDVGWLAYAFHVTTYLTFVEFFIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL
Sbjct: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy TrEMBL
Match: A0A6J1D4I4 (delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016898 PE=3 SV=1)

HSP 1 Score: 535 bits (1377), Expect = 1.15e-191
Identity = 234/274 (85.40%), Postives = 258/274 (94.16%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGGRPPYLELFLEET+ YNRI+LG+IFP F  D  PHI+QTWLRNCIGGFLIYFFSGFLW
Sbjct: 1   MGGRPPYLELFLEETTFYNRIILGSIFPAFASDQLPHIVQTWLRNCIGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CF+IYYWKRNVYVPKDSIPSN+ MLLQI+ T+ AMPLYC+LPTFSEYVVEHDWT+CYPR+
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQIYVTITAMPLYCILPTFSEYVVEHDWTKCYPRI 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
            DVGWLAYAFH+ +YLTFVEFSIYWMHR LHD+KPLYKYLHAKHH+YNKK+TLSPFAGLA
Sbjct: 121 RDVGWLAYAFHIISYLTFVEFSIYWMHRGLHDIKPLYKYLHAKHHIYNKKSTLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHP+DGILQAIPHLFALFLIP HFRTH++LLF EVVWTTNIHDCIHA++WPVMGAGYHT+
Sbjct: 181 FHPIDGILQAIPHLFALFLIPTHFRTHVVLLFLEVVWTTNIHDCIHAELWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDP DT+ K+S
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPKDTEAKVS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy TrEMBL
Match: A0A0A0L3B9 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G409800 PE=3 SV=1)

HSP 1 Score: 533 bits (1372), Expect = 6.66e-191
Identity = 238/274 (86.86%), Postives = 256/274 (93.43%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGG PPYLELF+EETS YNRIVLGTIFP+F LDPFPHILQTWLRNC+GGFLIYFFSGFLW
Sbjct: 1   MGGGPPYLELFVEETSFYNRIVLGTIFPNFSLDPFPHILQTWLRNCVGGFLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CF+IYYWKRNVYVPKDSIPSN+ MLLQI  T+KAMPLYCVLPTF+EYVVEHDWT+CYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKSMLLQILVTIKAMPLYCVLPTFAEYVVEHDWTKCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
            DVGW AYAFH+ TYLTF+EF IYWMHR LHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 LDVGWPAYAFHIITYLTFIEFCIYWMHRGLHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHP+DGILQAIPHLFALFLIP HFRTHI+LLFFEVVWT NIHD IH++MWPVMGAGYHT+
Sbjct: 181 FHPMDGILQAIPHLFALFLIPTHFRTHIVLLFFEVVWTANIHDGIHSRMWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDPMD + K S
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDIEAKGS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. ExPASy TrEMBL
Match: A0A6J1GYF7 (delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111457984 PE=3 SV=1)

HSP 1 Score: 533 bits (1372), Expect = 6.66e-191
Identity = 236/274 (86.13%), Postives = 254/274 (92.70%), Query Frame = 0


Query: 1   MGGRPPYLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLW 60
           MGG  PYLELF+ ETS YNRIVLGTIFP F LDPFPHI+QTWLRNCIGG LIYFFSGFLW
Sbjct: 1   MGGGQPYLELFVAETSFYNRIVLGTIFPAFTLDPFPHIVQTWLRNCIGGLLIYFFSGFLW 60

Query: 61  CFFIYYWKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRV 120
           CF+IYYWKRNVYVPKDSIPSN+LML Q+  T+KAMP YC+LPTF+EYVVEHDWT+CYPRV
Sbjct: 61  CFYIYYWKRNVYVPKDSIPSNKLMLFQMFVTIKAMPFYCILPTFAEYVVEHDWTKCYPRV 120

Query: 121 FDVGWLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLA 180
            DVGW AYAFH+ TYL FVEF IYWMHRALHD+KPLYKYLHAKHH+YNKK TLSPFAGLA
Sbjct: 121 LDVGWRAYAFHIVTYLAFVEFCIYWMHRALHDIKPLYKYLHAKHHIYNKKITLSPFAGLA 180

Query: 181 FHPVDGILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTL 240
           FHPVDGILQAIPHLFALFLIP HFRTH+MLLFFEVVWTTNIHDCIHAQ+WPVMGAGYHT+
Sbjct: 181 FHPVDGILQAIPHLFALFLIPTHFRTHVMLLFFEVVWTTNIHDCIHAQIWPVMGAGYHTI 240

Query: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPMDTQHKLS 274
           HHTRYRYNYGHYSIWMDWMFGTLLDP+DT+ + S
Sbjct: 241 HHTRYRYNYGHYSIWMDWMFGTLLDPIDTEARGS 274

[Close]

BLAST of Cp4.1LG02g13820 vs. TAIR 10
Match: AT3G02580.1 (sterol 1 )

HSP 1 Score: 387.9 bits (995), Expect = 6.8e-108
Identity = 169/261 (64.75%), Postives = 208/261 (79.69%), Query Frame = 0


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           YL  F++ETS YNRIVL  + P  + +P PH LQTWLRN + G L+YF SGFLWCF+IYY
Sbjct: 7   YLMQFVDETSFYNRIVLSHLLPANLWEPLPHFLQTWLRNYLAGTLLYFISGFLWCFYIYY 66

Query: 67  WKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVY+PKD+IP+ + M LQ+   +KAMP Y +LPT SE ++E  WT+C+  + + GW+
Sbjct: 67  LKINVYLPKDAIPTIKAMRLQMFVAMKAMPWYTLLPTVSESMIERGWTKCFASIGEFGWI 126

Query: 127 AYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
            Y  ++  YL FVEF IYWMHR LHD+KPLYKYLHA HH+YNK+ TLSPFAGLAFHPVDG
Sbjct: 127 LYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPVDG 186

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQA+PH+ ALF++PIHF THI LLF E +WT NIHDCIH  +WPVMGAGYHT+HHT Y+
Sbjct: 187 ILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVMGAGYHTIHHTTYK 246

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L DP+
Sbjct: 247 HNYGHYTIWMDWMFGSLRDPL 267

[Close]

BLAST of Cp4.1LG02g13820 vs. TAIR 10
Match: AT3G02590.1 (Fatty acid hydroxylase superfamily protein )

HSP 1 Score: 366.7 bits (940), Expect = 1.6e-101
Identity = 159/261 (60.92%), Postives = 202/261 (77.39%), Query Frame = 0


Query: 7   YLELFLEETSLYNRIVLGTIFPDFVLDPFPHILQTWLRNCIGGFLIYFFSGFLWCFFIYY 66
           Y +  + ETS YNR+VL  + P  + +P PH LQTWLRN + G ++YF SGFLWCF+IYY
Sbjct: 8   YNDQIVNETSFYNRMVLSHLLPVNLWEPLPHFLQTWLRNYLAGNILYFISGFLWCFYIYY 67

Query: 67  WKRNVYVPKDSIPSNRLMLLQIHATVKAMPLYCVLPTFSEYVVEHDWTRCYPRVFDVGWL 126
            K NVYVPK+SIP+ + MLLQI+  +KAMP Y +LP  SEY++EH WT+CY  +    W 
Sbjct: 68  LKLNVYVPKESIPTRKAMLLQIYVAMKAMPWYTLLPAVSEYMIEHGWTKCYSTLDHFNWF 127

Query: 127 AYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG 186
               ++  YL  VEF IYW+H+ LHD+K LYK+LHA HHMYNK+ TLSPFAGLAFHP+DG
Sbjct: 128 LCFLYIALYLVLVEFMIYWVHKELHDIKFLYKHLHATHHMYNKQNTLSPFAGLAFHPLDG 187

Query: 187 ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLHHTRYR 246
           ILQAIPH+ ALF++PIH  TH+ LLF E +WT +IHDCIH  +WP+MGAGYHT+HHT Y+
Sbjct: 188 ILQAIPHVIALFIVPIHLITHLSLLFLEGIWTASIHDCIHGNIWPIMGAGYHTIHHTTYK 247

Query: 247 YNYGHYSIWMDWMFGTLLDPM 268
           +NYGHY+IWMDWMFG+L+ P+
Sbjct: 248 HNYGHYTIWMDWMFGSLMVPL 268

[Close]

BLAST of Cp4.1LG02g13820 vs. TAIR 10
Match: AT1G02205.2 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 49.7 bits (117), Expect = 4.4e-06
Identity = 41/151 (27.15%), Postives = 72/151 (47.68%), Query Frame = 0


Query: 139 VEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG-----ILQAIPH 198
           VEF  YW+H+ALH     + +L++++H ++  + ++       HP        IL AIP 
Sbjct: 138 VEFLYYWLHKALH-----HHFLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFILFAIPL 197

Query: 199 L------------FALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLH 258
           L            FA ++I I F  ++    FE++     H       +      YH+LH
Sbjct: 198 LTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFH-LFPPLKFLCYTPSYHSLH 257

Query: 259 HTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
           HT++R NY  +    D+++GT+ +  DT ++
Sbjct: 258 HTQFRTNYSLFMPLYDYIYGTMDESTDTLYE 282

[Close]

BLAST of Cp4.1LG02g13820 vs. TAIR 10
Match: AT1G02205.3 (Fatty acid hydroxylase superfamily )

HSP 1 Score: 49.7 bits (117), Expect = 4.4e-06
Identity = 41/151 (27.15%), Postives = 72/151 (47.68%), Query Frame = 0


Query: 139 VEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFAGLAFHPVDG-----ILQAIPH 198
           VEF  YW+H+ALH     + +L++++H ++  + ++       HP        IL AIP 
Sbjct: 138 VEFLYYWLHKALH-----HHFLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFILFAIPL 197

Query: 199 L------------FALFLIPIHFRTHIMLLFFEVVWTTNIHDCIHAQMWPVMGAGYHTLH 258
           L            FA ++I I F  ++    FE++     H       +      YH+LH
Sbjct: 198 LTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFH-LFPPLKFLCYTPSYHSLH 257

Query: 259 HTRYRYNYGHYSIWMDWMFGTLLDPMDTQHK 273
           HT++R NY  +    D+++GT+ +  DT ++
Sbjct: 258 HTQFRTNYSLFMPLYDYIYGTMDESTDTLYE 282

[Close]

BLAST of Cp4.1LG02g13820 vs. TAIR 10
Match: AT1G69640.1 (sphingoid base hydroxylase 1 )

HSP 1 Score: 42.0 bits (97), Expect = 9.2e-04
Identity = 41/153 (26.80%), Postives = 74/153 (48.37%), Query Frame = 0


Query: 125 WLAYAFHVTTYLTFVEFSIYWMHRALHDVKPLYKYLHAKHHMYNKKATLSPFA--GLAFH 184
           +L  A    T +  ++   Y+MHR +H  K LYK++H++HH       + P+A   L  H
Sbjct: 92  FLVLARQFVTAMIVLDTWQYFMHRYMHQNKFLYKHIHSQHH-----RLIVPYAYGALYNH 151

Query: 185 PVDG-ILQAIPHLFALFLIPIHFRTHIMLLFFEVVWTTNIHDC---IHAQMWPVM---GA 244
           PV+G +L  I    +  +  +  RT I    F  + T + H C   +   ++ ++    +
Sbjct: 152 PVEGLLLDTIGGALSFLVSGMSPRTSIFFFSFATIKTVDDH-CGLWLPGNLFHMVFKNNS 211

Query: 245 GYHTLHH----TRYRYNYGHYSIWMDWMFGTLL 265
            YH +HH    T+Y ++   + +W D + GT +
Sbjct: 212 AYHDIHHQLYGTKYNFSQPFFVMW-DRILGTYM 237

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g13820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZT29	1.5e-112	66.54	Delta(7)-sterol-C5(6)-desaturase OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
Q39208	9.6e-107	64.75	Delta(7)-sterol-C5(6)-desaturase 1 OS=Arabidopsis thaliana OX=3702 GN=STE1 PE=1 ...	[more]
Q9M883	2.3e-100	60.92	Putative Delta(7)-sterol-C5(6)-desaturase 2 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]
Q9EQS5	5.1e-23	32.19	Lathosterol oxidase OS=Rattus norvegicus OX=10116 GN=Sc5d PE=1 SV=1	[more]
O88822	3.3e-22	31.76	Lathosterol oxidase OS=Mus musculus OX=10090 GN=Sc5d PE=2 SV=2	[more]

BLAST of Cp4.1LG02g13820 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023525270.1	9.53e-216	100.00	delta(7)-sterol-C5(6)-desaturase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6607054.1	2.73e-215	99.64	hypothetical protein SDJN03_00396, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022948870.1	3.88e-215	99.27	delta(7)-sterol-C5(6)-desaturase-like [Cucurbita moschata] >KAG7036755.1 hypothe...	[more]
XP_022998219.1	3.19e-214	98.91	delta(7)-sterol-C5(6)-desaturase-like [Cucurbita maxima]	[more]
XP_038894105.1	5.00e-193	86.86	delta(7)-sterol-C5(6)-desaturase-like [Benincasa hispida] >XP_038894106.1 delta(...	[more]

BLAST of Cp4.1LG02g13820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GB39	1.88e-215	99.27	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111452...	[more]
A0A6J1KDR9	1.55e-214	98.91	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC11149293...	[more]
A0A6J1D4I4	1.15e-191	85.40	delta(7)-sterol-C5(6)-desaturase-like isoform X1 OS=Momordica charantia OX=3673 ...	[more]
A0A0A0L3B9	6.66e-191	86.86	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1GYF7	6.66e-191	86.13	delta(7)-sterol-C5(6)-desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111457...	[more]

BLAST of Cp4.1LG02g13820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G02580.1	6.8e-108	64.75	sterol 1	[more]
AT3G02590.1	1.6e-101	60.92	Fatty acid hydroxylase superfamily protein	[more]
AT1G02205.2	4.4e-06	27.15	Fatty acid hydroxylase superfamily	[more]
AT1G02205.3	4.4e-06	27.15	Fatty acid hydroxylase superfamily	[more]
AT1G69640.1	9.2e-04	26.80	sphingoid base hydroxylase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 134..262 e-value: 4.3E-20 score: 72.4
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 9..268
None	No IPR available	PANTHER	PTHR11863:SF160	DELTA(7)-STEROL-C5(6)-DESATURASE	coord: 9..268

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g13820.1	Cp4.1LG02g13820.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0005789	endoplasmic reticulum membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0000248	C-5 sterol desaturase activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity