Cp4.1LG02g13770 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g13770
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description2-hydroxyacyl-CoA lyase-like
LocationCp4.1LG02: 12265643 .. 12268827 (+)
RNA-Seq ExpressionCp4.1LG02g13770
SyntenyCp4.1LG02g13770
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTAAGATATTTTATTTTTGCCCTTTTCTCACTCTTCCTCACACTTCCATTGCTTCCCACCGGCAAAATCTCAGAGCTTCGATGGGGGATTCCGCCGGAGATGTTAAAACCGACCTCAAATTTCATGGTCCGATCGACGGCAATGTTCTCGCTGCCATGGCGCTCGCACGTTTCGGTGTCGACCGGATGTTTGGTGTCGTCGGAATTCCTGTTACCTCTCTGGCAACGCGCGCCGTCTCTCTTGGAATCCGATTCATCGCCTTTCATAACGAACAATCGGCTGGCTATGCTGCTTCTGCTTATGGATATCTAACGGGACGTCCTGGTGTGCTTCTTACTGTTTCTGGCCCTGGGTGTGTTCATGGCCTTGCGGGGGTTTCTAACGCCATGGCTAATGCTTGGCCTCTTGTGATGATCTCTGGTTCTTGTGATCAGAGGGATTTTGGCCGTGGTGATTTTCAGGAGCTTGATCAGATTGAGGCTGTGAAGCCCTTTTCGAAATTTTCCGTCAAGGCTACGGATATTTCCGAGATTCCGAATTGTGTCGCTCGAGTTCTTAATTCTGCCGTTTCTGGTCGGCCTGGTGGATGCTATTTTGATCTTCCGTCTGATGTTTTGCACCAGACTGTTTCCGAGTCTGAGGCGGAGAGATTATTGGCTGCGGCGGAGGAATTTTCACATCGCGAGGTTATTCATAGAGTTCCGAATTCGCAGATTGAGGAAGCGATTTCGTTACTGAAACATGCTGAACGGCCATTGATTGTGTTTGGTAAAGGGGCGGCGATCGCTCGGGCTGAGGGGCCGTTGAAGAAGCTTGTGGAGAGCACTGGGATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTGTTGCCAGATACACATCAGCTAGCGGCTACAGCCGCTAGGTCGTTGGCGATTGGCAAGTGTGATGTTGCGCTTGTGGTTGGGGCACGGCTTAATTGGCTGTTGCATTTTGGCGAGCCGCCTAAATGGTCCAAGGATGTGAAGTTCATTTTGGTTGATATTTGCAAGGAAGAGGTTGAATTGAGAAAGCCCCATTTGGGTTTGATTGGCGATGCAAAACAGATATTGGAGTCCATTAACAAGGAGATTAAGGATGATCCCTTTTGTCTTGGGAAGTCTCACCCGTGGGTTGAAGCCATTTCACAGAAGGCAAGGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAGGGATGTTGTGCCGTTCAATTTCATGACTCCTATGAGGATCATTAGAGATGCAATCTCGGCTGTTGGTAGCCCTTCTCCCATATTGGTCTCTGAAGGAGCCAATACAATGGATGTGGGTCGCTCAGTTTTGGTTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACATGGGGTACAATGGGGGTTGGCTTGGGCTATTGCATTGCTGCTGCTGTCGCTTCGCCCGATCGATTGGTAGTTGCTGTGGAAGGCGATTCTGGATTCGGGTTTAGCGCCATGGAAGTTGAGGTATTCATTCCCCCCTTTACTCTTTCTTTTCATAATTGCAATTGATTAGAAGGTTTTATGTTCAGTGTCTGATCTATTAGCCATTTGAAGAGTGCCCTGCATATCAAAACTCGGTGTGGTTGGCTCAAAATATTGCCCGAGTAATGGTTACTTTTTGGTTGGAAATGTTTGTTTGTTCGTTACTATGTTTCATTTAGCTGTATGACTGAGGTCAAAAAACCATGTAGACACTGGCAATCAGTAGTTCCAGGACTATTCTTGGACATGTTTTGTAGGCTGTGTTACTCCCTTGTTATGATGACCTCAAATTATTTAACATCCTAAGTAGGTGTAACGGCTCAAGTTCACCGCTAGCAGATATTGTCCTATTTGGGTTTTTCCTTTCGGACTTCCCCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGTAGTTTTCACACTCTTATAAAGAATGCTTCATTCTTCTCCTCCCCAACTGATGTGGGACCTCACAATCCACTCCCTTCGGGCCTAGCGGATCTCACAGTAGGTACCGTAGTATTATATACCGATTTGCCATCATTCAAATCTGAAGTTAAGATTATGCTTTTAAGTGATATAACTTGGTAGGTTGTGGACTTTTTTTAAACCTCTGTTGAAGCTTTAGATGATGTTTCTGTATCTGACTTCTGTTTGTAAAGTTGTACATCTTACTTTGAGTAGGGCTTTATTGCAAGTAGAATGAAGGTCTCTCTTGTGTTCTTTCTGAAGAACTCCTTATAATCTTTTAATTGGGCCAGTGTTGATTCTAATCTCAAAACATAATCCATTCGATATCTCAGAAACAAACAGTTGGGTTCTTTACTTTCCTCTTTTCTAATGTCTCTATGCCTGTCTTTTCAATTCAACTTCGAGTCGAGACGACTTTTGAGCTTTCGTTCTGTAAATGTCAAAGCATTTTCCTTTTTTTGTCTTTTTCTTTTTTCATGTGTTTGCTAATGTCATTGTACATATTTCTGGTTTTGAACAGACTCTTGTTCGATACCAGTTGCCAGTGGTCGTGATAGTTTTCAACAATGGCGGTGTCTATGGTGGTGACCGGAGAACACCGCAAGAAATTACCGGACCCTATAAGCATGACCCTGCACCAACCTCATTTGTCCCATCAGCAAATTATCACAAAATGATTGAAGCTTTTGGAGGCAAAGGATACTATGTCGAAACGCCTGAAGAACTAAAGTCTGCACTATCTGAGTCCTTTTCTGCTCGAAATCCAGCAGTCATAAATGTGAAGATCGATCCCTACGCCGGTGCAGAAAGCGGGAGGCTACAACATAAGAACTGAAGATAAGTCTTTAAACTCCTTTCAATTGTGTGCCAATGCATTATGTTTGTAGCAGAATTTAAAGCAAGCTTAGCTTTCTCTTCTTCTTTTCTTTTTCTTTTTCTATTTCATTTTCATTACCACTAAATTATGTAAAATTATGAACTTTGAATAAAGTTGAATGTAAGTTGTAAAGCCAGCAGTTGGAGACGGCTGTGTAGTTTGATAAATGATAAAATAGAAGTCTCTGTTCTAGTTTCTTAGTGGGAAGCAGTGGTATAACTTATTAATTAGATGAGTCAGACCCCATTTTGTAGAATGAGATAGAAATTGCTATCAAACATATGCACACACCAAAGCCATGAAGATCAATGCCTT

mRNA sequence

AAATTTAAGATATTTTATTTTTGCCCTTTTCTCACTCTTCCTCACACTTCCATTGCTTCCCACCGGCAAAATCTCAGAGCTTCGATGGGGGATTCCGCCGGAGATGTTAAAACCGACCTCAAATTTCATGGTCCGATCGACGGCAATGTTCTCGCTGCCATGGCGCTCGCACGTTTCGGTGTCGACCGGATGTTTGGTGTCGTCGGAATTCCTGTTACCTCTCTGGCAACGCGCGCCGTCTCTCTTGGAATCCGATTCATCGCCTTTCATAACGAACAATCGGCTGGCTATGCTGCTTCTGCTTATGGATATCTAACGGGACGTCCTGGTGTGCTTCTTACTGTTTCTGGCCCTGGGTGTGTTCATGGCCTTGCGGGGGTTTCTAACGCCATGGCTAATGCTTGGCCTCTTGTGATGATCTCTGGTTCTTGTGATCAGAGGGATTTTGGCCGTGGTGATTTTCAGGAGCTTGATCAGATTGAGGCTGTGAAGCCCTTTTCGAAATTTTCCGTCAAGGCTACGGATATTTCCGAGATTCCGAATTGTGTCGCTCGAGTTCTTAATTCTGCCGTTTCTGGTCGGCCTGGTGGATGCTATTTTGATCTTCCGTCTGATGTTTTGCACCAGACTGTTTCCGAGTCTGAGGCGGAGAGATTATTGGCTGCGGCGGAGGAATTTTCACATCGCGAGGTTATTCATAGAGTTCCGAATTCGCAGATTGAGGAAGCGATTTCGTTACTGAAACATGCTGAACGGCCATTGATTGTGTTTGGTAAAGGGGCGGCGATCGCTCGGGCTGAGGGGCCGTTGAAGAAGCTTGTGGAGAGCACTGGGATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTGTTGCCAGATACACATCAGCTAGCGGCTACAGCCGCTAGGTCGTTGGCGATTGGCAAGTGTGATGTTGCGCTTGTGGTTGGGGCACGGCTTAATTGGCTGTTGCATTTTGGCGAGCCGCCTAAATGGTCCAAGGATGTGAAGTTCATTTTGGTTGATATTTGCAAGGAAGAGGTTGAATTGAGAAAGCCCCATTTGGGTTTGATTGGCGATGCAAAACAGATATTGGAGTCCATTAACAAGGAGATTAAGGATGATCCCTTTTGTCTTGGGAAGTCTCACCCGTGGGTTGAAGCCATTTCACAGAAGGCAAGGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAGGGATGTTGTGCCGTTCAATTTCATGACTCCTATGAGGATCATTAGAGATGCAATCTCGGCTGTTGGTAGCCCTTCTCCCATATTGGTCTCTGAAGGAGCCAATACAATGGATGTGGGTCGCTCAGTTTTGGTTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACATGGGGTACAATGGGGGTTGGCTTGGGCTATTGCATTGCTGCTGCTGTCGCTTCGCCCGATCGATTGGTAGTTGCTGTGGAAGGCGATTCTGGATTCGGGTTTAGCGCCATGGAAGTTGAGACTCTTGTTCGATACCAGTTGCCAGTGGTCGTGATAGTTTTCAACAATGGCGGTGTCTATGGTGGTGACCGGAGAACACCGCAAGAAATTACCGGACCCTATAAGCATGACCCTGCACCAACCTCATTTGTCCCATCAGCAAATTATCACAAAATGATTGAAGCTTTTGGAGGCAAAGGATACTATGTCGAAACGCCTGAAGAACTAAAGTCTGCACTATCTGAGTCCTTTTCTGCTCGAAATCCAGCAGTCATAAATGTGAAGATCGATCCCTACGCCGGTGCAGAAAGCGGGAGGCTACAACATAAGAACTGAAGATAAGTCTTTAAACTCCTTTCAATTGTGTGCCAATGCATTATGTTTGTAGCAGAATTTAAAGCAAGCTTAGCTTTCTCTTCTTCTTTTCTTTTTCTTTTTCTATTTCATTTTCATTACCACTAAATTATGTAAAATTATGAACTTTGAATAAAGTTGAATGTAAGTTGTAAAGCCAGCAGTTGGAGACGGCTGTGTAGTTTGATAAATGATAAAATAGAAGTCTCTGTTCTAGTTTCTTAGTGGGAAGCAGTGGTATAACTTATTAATTAGATGAGTCAGACCCCATTTTGTAGAATGAGATAGAAATTGCTATCAAACATATGCACACACCAAAGCCATGAAGATCAATGCCTT

Coding sequence (CDS)

AAATTTAAGATATTTTATTTTTGCCCTTTTCTCACTCTTCCTCACACTTCCATTGCTTCCCACCGGCAAAATCTCAGAGCTTCGATGGGGGATTCCGCCGGAGATGTTAAAACCGACCTCAAATTTCATGGTCCGATCGACGGCAATGTTCTCGCTGCCATGGCGCTCGCACGTTTCGGTGTCGACCGGATGTTTGGTGTCGTCGGAATTCCTGTTACCTCTCTGGCAACGCGCGCCGTCTCTCTTGGAATCCGATTCATCGCCTTTCATAACGAACAATCGGCTGGCTATGCTGCTTCTGCTTATGGATATCTAACGGGACGTCCTGGTGTGCTTCTTACTGTTTCTGGCCCTGGGTGTGTTCATGGCCTTGCGGGGGTTTCTAACGCCATGGCTAATGCTTGGCCTCTTGTGATGATCTCTGGTTCTTGTGATCAGAGGGATTTTGGCCGTGGTGATTTTCAGGAGCTTGATCAGATTGAGGCTGTGAAGCCCTTTTCGAAATTTTCCGTCAAGGCTACGGATATTTCCGAGATTCCGAATTGTGTCGCTCGAGTTCTTAATTCTGCCGTTTCTGGTCGGCCTGGTGGATGCTATTTTGATCTTCCGTCTGATGTTTTGCACCAGACTGTTTCCGAGTCTGAGGCGGAGAGATTATTGGCTGCGGCGGAGGAATTTTCACATCGCGAGGTTATTCATAGAGTTCCGAATTCGCAGATTGAGGAAGCGATTTCGTTACTGAAACATGCTGAACGGCCATTGATTGTGTTTGGTAAAGGGGCGGCGATCGCTCGGGCTGAGGGGCCGTTGAAGAAGCTTGTGGAGAGCACTGGGATTCCATTTCTTCCTACTCCAATGGGTAAAGGATTGTTGCCAGATACACATCAGCTAGCGGCTACAGCCGCTAGGTCGTTGGCGATTGGCAAGTGTGATGTTGCGCTTGTGGTTGGGGCACGGCTTAATTGGCTGTTGCATTTTGGCGAGCCGCCTAAATGGTCCAAGGATGTGAAGTTCATTTTGGTTGATATTTGCAAGGAAGAGGTTGAATTGAGAAAGCCCCATTTGGGTTTGATTGGCGATGCAAAACAGATATTGGAGTCCATTAACAAGGAGATTAAGGATGATCCCTTTTGTCTTGGGAAGTCTCACCCGTGGGTTGAAGCCATTTCACAGAAGGCAAGGGACAATGTGGCGAAAATGGAAGTTCAGCTGGCGAGGGATGTTGTGCCGTTCAATTTCATGACTCCTATGAGGATCATTAGAGATGCAATCTCGGCTGTTGGTAGCCCTTCTCCCATATTGGTCTCTGAAGGAGCCAATACAATGGATGTGGGTCGCTCAGTTTTGGTTCAGACTGAGCCAAGGACCAGGTTGGATGCAGGAACATGGGGTACAATGGGGGTTGGCTTGGGCTATTGCATTGCTGCTGCTGTCGCTTCGCCCGATCGATTGGTAGTTGCTGTGGAAGGCGATTCTGGATTCGGGTTTAGCGCCATGGAAGTTGAGACTCTTGTTCGATACCAGTTGCCAGTGGTCGTGATAGTTTTCAACAATGGCGGTGTCTATGGTGGTGACCGGAGAACACCGCAAGAAATTACCGGACCCTATAAGCATGACCCTGCACCAACCTCATTTGTCCCATCAGCAAATTATCACAAAATGATTGAAGCTTTTGGAGGCAAAGGATACTATGTCGAAACGCCTGAAGAACTAAAGTCTGCACTATCTGAGTCCTTTTCTGCTCGAAATCCAGCAGTCATAAATGTGAAGATCGATCCCTACGCCGGTGCAGAAAGCGGGAGGCTACAACATAAGAACTGA

Protein sequence

KFKIFYFCPFLTLPHTSIASHRQNLRASMGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN
Homology
BLAST of Cp4.1LG02g13770 vs. ExPASy Swiss-Prot
Match: Q9LF46 (2-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 5.0e-265
Identity = 445/561 (79.32%), Postives = 500/561 (89.13%), Query Frame = 0

Query: 46  IDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 105
           IDGNVL A +L+  GV  MFGVVGIPVTSLA+RA++LGIRFIAFHNEQSAGYAASAYGYL
Sbjct: 12  IDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMALGIRFIAFHNEQSAGYAASAYGYL 71

Query: 106 TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQIEAVKP 165
           TG+PG+LLTVSGPGCVHGLAG+SNA  N WP+VMISGSCDQRD GRGDFQELDQIEAVK 
Sbjct: 72  TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQIEAVKA 131

Query: 166 FSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLAAAEE 225
           FSK S KA D+ EIP+CV+RVL+ AVSGRPGGCY D+P+DVL Q +SESEA++L+   E 
Sbjct: 132 FSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKLVDEVER 191

Query: 226 FSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTP 285
               E I     S+IE A+SLL+ AERPLIVFGKGAA +RAE  LKKLVE TGIPFLPTP
Sbjct: 192 SRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPTP 251

Query: 286 MGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICK 345
           MGKGLLPDTH+ +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFILVD+ +
Sbjct: 252 MGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFILVDVSE 311

Query: 346 EEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAKMEVQLA 405
           EE+ELRKPHLG++GDAK ++  +N+EIKDDPFCLGKS+ WVE+IS+KA++N  KME+QLA
Sbjct: 312 EEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEKMEIQLA 371

Query: 406 RDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGT 465
           +DVVPFNF+TPMRIIRDAI AV  PSP++VSEGANTMDVGRSVLVQ EPRTRLDAGTWGT
Sbjct: 372 KDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGT 431

Query: 466 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYGGD 525
           MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY L VV+IVFNNGGVYGGD
Sbjct: 432 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNGGVYGGD 491

Query: 526 RRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESFSARNPA 585
           RR P+EI+GP+K DPAPTSFVP+A YHK+IEAFGGKGY VETP+ELKSAL+ESF+AR PA
Sbjct: 492 RRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESFAARKPA 551

Query: 586 VINVKIDPYAGAESGRLQHKN 607
           V+NV IDP+AGAESGRLQHKN
Sbjct: 552 VVNVIIDPFAGAESGRLQHKN 572

BLAST of Cp4.1LG02g13770 vs. ExPASy Swiss-Prot
Match: Q0JMH0 (2-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400 PE=3 SV=3)

HSP 1 Score: 885.2 bits (2286), Expect = 4.2e-256
Identity = 430/565 (76.11%), Postives = 493/565 (87.26%), Query Frame = 0

Query: 46  IDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 105
           +DG+ LA  ALA  G   MFGVVGIPVTSLA+RA + G+RF+AF NEQSAGYAA+AYG+L
Sbjct: 13  VDGSALAGRALAAAGARHMFGVVGIPVTSLASRAAAAGVRFLAFRNEQSAGYAAAAYGFL 72

Query: 106 TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQIEAVKP 165
           TG PG+LLTVSGPGCVHGLAG+S+A ANAWPL+M+SGSC Q D GRGDFQELDQI A KP
Sbjct: 73  TGSPGLLLTVSGPGCVHGLAGLSHATANAWPLLMVSGSCSQPDAGRGDFQELDQIAATKP 132

Query: 166 FSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLAAAEE 225
           F K +VKAT I++IP  V + L + VSGRPGGCY D+PSDVLHQT++ESEA  L+ AA  
Sbjct: 133 FIKIAVKATTIADIPRLVFQALAATVSGRPGGCYLDIPSDVLHQTLTESEAAALIDAAAA 192

Query: 226 FSHRE----VIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGIPF 285
            S +       H+  +  IE+A  LL+ AERPL+VFGKGAA +RAE  + KLV++TGIPF
Sbjct: 193 DSAKSDSSPPKHKSLDEGIEKAAELLRRAERPLVVFGKGAAYSRAEDAIWKLVDTTGIPF 252

Query: 286 LPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILV 345
           LPTPMGKG++PDTH L+ATAARSLAIG+CDVALVVGARLNWLLHFGEPPKWSKDVKFILV
Sbjct: 253 LPTPMGKGVVPDTHPLSATAARSLAIGQCDVALVVGARLNWLLHFGEPPKWSKDVKFILV 312

Query: 346 DICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAKME 405
           D+C+EE+ELRKPH+G++GDAK+++E IN+EIKD PFCL  SHPWVEAI++KARDNV KME
Sbjct: 313 DVCEEEIELRKPHVGIVGDAKRVVELINREIKDQPFCLAPSHPWVEAITKKARDNVLKME 372

Query: 406 VQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSVLVQTEPRTRLDAG 465
            QLA+DVVPFNF+TP+RIIRDAI A G+P+P++VSEGANTMDVGR+VLVQ EPRTRLDAG
Sbjct: 373 AQLAKDVVPFNFLTPLRIIRDAILAEGNPAPVVVSEGANTMDVGRAVLVQNEPRTRLDAG 432

Query: 466 TWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGV 525
           TWGTMGVGLG+C+AAAVA PDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNN GV
Sbjct: 433 TWGTMGVGLGFCVAAAVAEPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNNGV 492

Query: 526 YGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESFSA 585
           YGGDRR+P EITGPYK DPAPTSFVP+A YHKM+EAFGGKGY VETP+ELKSALSESF A
Sbjct: 493 YGGDRRSPDEITGPYKDDPAPTSFVPAAGYHKMMEAFGGKGYLVETPDELKSALSESFRA 552

Query: 586 RNPAVINVKIDPYAGAESGRLQHKN 607
           R PAVINV IDPYAGAESGR+QHKN
Sbjct: 553 RKPAVINVIIDPYAGAESGRMQHKN 577

BLAST of Cp4.1LG02g13770 vs. ExPASy Swiss-Prot
Match: Q8CHM7 (2-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 2.0e-128
Identity = 245/558 (43.91%), Postives = 352/558 (63.08%), Query Frame = 0

Query: 46  IDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 105
           + G  + A AL    V+ MFGVVGIPVT +A  A  LGI++I   NEQ+A YAASA GYL
Sbjct: 17  VSGAKVIAQALKTQDVEYMFGVVGIPVTEIALAAQELGIKYIGMRNEQAACYAASAVGYL 76

Query: 106 TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQIEAVKP 165
           TGRPGV L VSGPG +H L G++NA  N WPL++I GS ++     G FQE  Q+EA + 
Sbjct: 77  TGRPGVCLVVSGPGLIHALGGMANANMNCWPLIVIGGSSERNQEAMGAFQEFPQVEACRL 136

Query: 166 FSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLAAAEE 225
           +SKFS + + I  IP  + + + S++ GRPG CY D+P+D++  T+ ES         +E
Sbjct: 137 YSKFSARPSSIQHIPFVIEKAVRSSIYGRPGACYIDVPADLV--TLQESTTS---IKYKE 196

Query: 226 FSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTP 285
                 +     S +  A S+L+ A++PL++ GKGAA + AE  ++KLVE   +PFLPTP
Sbjct: 197 CCMPPPLSLAETSAVRAAASVLRSAKQPLLIIGKGAAYSHAEDSIRKLVELCNLPFLPTP 256

Query: 286 MGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICK 345
           MGKG++PD H     AARS A+   DV ++ GARLNW+LHFG PP++  DVKFI +DIC 
Sbjct: 257 MGKGVVPDNHPNCVGAARSRALQFADVIVLFGARLNWILHFGLPPRYQADVKFIQIDICA 316

Query: 346 EEVELR-KPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAKMEVQL 405
           EE+    +P + L+GD   + + + ++   +P+       W E + +K ++N A  +   
Sbjct: 317 EELGNNVRPSVTLLGDVNAVSKQLLEQFVKNPWQYPTDSKWWETLREKRKNNEAVSKELA 376

Query: 406 ARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWG 465
           ++  +P N+ T    +++ +      +  +VSEGANTMD+GR+VL    PR RLDAG++G
Sbjct: 377 SKKSLPMNYYTVFYHVQEQLPR----NCFIVSEGANTMDIGRTVLQNYLPRHRLDAGSFG 436

Query: 466 TMGVGLGYCIAAAVA----SPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGG 525
           TMGVGLG+ IAAAV     SP + V+ VEGDS FGFS MEVET+ RY LP++++V NN G
Sbjct: 437 TMGVGLGFAIAAAVVAKERSPGQRVICVEGDSAFGFSGMEVETICRYNLPIIILVVNNNG 496

Query: 526 VYGG-DRRTPQEI---TGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALS 585
           +Y G D  T  +I    G     P P   +P+++Y +++ AFGGKGY+V+TPEEL+ +L 
Sbjct: 497 IYQGFDADTWGKILNFQGTATTIP-PMCLLPNSHYEQVMTAFGGKGYFVQTPEELQDSLR 556

Query: 586 ESF-SARNPAVINVKIDP 594
           ++      P +IN+ I+P
Sbjct: 557 QALKDTSKPCLINIMIEP 564

BLAST of Cp4.1LG02g13770 vs. ExPASy Swiss-Prot
Match: P0AFI1 (Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 5.8e-128
Identity = 246/563 (43.69%), Postives = 356/563 (63.23%), Query Frame = 0

Query: 47  DGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLT 106
           DG  +   AL +  +D ++GVVGIPVT +A  A + GIR+I F +EQSAGYAA+A G+LT
Sbjct: 9   DGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAASGFLT 68

Query: 107 GRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQR--DFGRGDFQELDQIEAVK 166
            +PG+ LTVS PG ++GL  ++NA  N +P++MISGS D+   D  +GD++ELDQ+ A K
Sbjct: 69  QKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAK 128

Query: 167 PFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLAAAE 226
           P++K + +     ++   +AR +  +VSGRPGG Y DLP++VL  T+ + EA   +   E
Sbjct: 129 PYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEALTTIVKVE 188

Query: 227 EFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPT 286
             S    +   P S +  AISLL  AERPLI+ GKGAA ++A+  L++ +ES  IPFLP 
Sbjct: 189 NPS--PALLPCPKS-VTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESAQIPFLPM 248

Query: 287 PMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDIC 346
            M KG+L DTH L+A AARS A+   DV ++VGARLNWLL  G+   W+ D +FI +DI 
Sbjct: 249 SMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGK-KGWAADTQFIQLDIE 308

Query: 347 KEEVELRKP-HLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAKMEVQ 406
            +E++  +P  + ++GD    ++ +  E+K + F       W + ++   + N  KM  +
Sbjct: 309 PQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFT--TPLVWRDILNIHKQQNAQKMHEK 368

Query: 407 LARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSVLVQTEPRTRLDAGTW 466
           L+ D  P N+   +  +RD +    +    LV+EGANT+D  R+++   +PR RLD GTW
Sbjct: 369 LSTDTQPLNYFNALSAVRDVLRE--NQDIYLVNEGANTLDNARNIIDMYKPRRRLDCGTW 428

Query: 467 GTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYG 526
           G MG+G+GY I A+V S    VVA+EGDS FGFS ME+ET+ RY LPV +++FNNGG+Y 
Sbjct: 429 GVMGIGMGYAIGASVTSGSP-VVAIEGDSAFGFSGMEIETICRYNLPVTIVIFNNGGIYR 488

Query: 527 GDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESFSARN 586
           GD     +++G     P+PT  +  A Y K+++AF G GY V T +EL+ AL+    +R 
Sbjct: 489 GD---GVDLSG--AGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTTGIQSRK 548

Query: 587 PAVINVKIDPYAGAESGRLQHKN 607
           P +INV IDP AG ESG +   N
Sbjct: 549 PTIINVVIDPAAGTESGHITKLN 557

BLAST of Cp4.1LG02g13770 vs. ExPASy Swiss-Prot
Match: P0AFI0 (Oxalyl-CoA decarboxylase OS=Escherichia coli (strain K12) OX=83333 GN=oxc PE=1 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 5.8e-128
Identity = 246/563 (43.69%), Postives = 356/563 (63.23%), Query Frame = 0

Query: 47  DGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLT 106
           DG  +   AL +  +D ++GVVGIPVT +A  A + GIR+I F +EQSAGYAA+A G+LT
Sbjct: 9   DGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAEGIRYIGFRHEQSAGYAAAASGFLT 68

Query: 107 GRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQR--DFGRGDFQELDQIEAVK 166
            +PG+ LTVS PG ++GL  ++NA  N +P++MISGS D+   D  +GD++ELDQ+ A K
Sbjct: 69  QKPGICLTVSAPGFLNGLTALANATVNGFPMIMISGSSDRAIVDLQQGDYEELDQMNAAK 128

Query: 167 PFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLAAAE 226
           P++K + +     ++   +AR +  +VSGRPGG Y DLP++VL  T+ + EA   +   E
Sbjct: 129 PYAKAAFRVNQPQDLGIALARAIRVSVSGRPGGVYLDLPANVLAATMEKDEALTTIVKVE 188

Query: 227 EFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPT 286
             S    +   P S +  AISLL  AERPLI+ GKGAA ++A+  L++ +ES  IPFLP 
Sbjct: 189 NPS--PALLPCPKS-VTSAISLLAKAERPLIILGKGAAYSQADEQLREFIESAQIPFLPM 248

Query: 287 PMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDIC 346
            M KG+L DTH L+A AARS A+   DV ++VGARLNWLL  G+   W+ D +FI +DI 
Sbjct: 249 SMAKGILEDTHPLSAAAARSFALANADVVMLVGARLNWLLAHGK-KGWAADTQFIQLDIE 308

Query: 347 KEEVELRKP-HLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAKMEVQ 406
            +E++  +P  + ++GD    ++ +  E+K + F       W + ++   + N  KM  +
Sbjct: 309 PQEIDSNRPIAVPVVGDIASSMQGMLAELKQNTFT--TPLVWRDILNIHKQQNAQKMHEK 368

Query: 407 LARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSVLVQTEPRTRLDAGTW 466
           L+ D  P N+   +  +RD +    +    LV+EGANT+D  R+++   +PR RLD GTW
Sbjct: 369 LSTDTQPLNYFNALSAVRDVLRE--NQDIYLVNEGANTLDNARNIIDMYKPRRRLDCGTW 428

Query: 467 GTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYG 526
           G MG+G+GY I A+V S    VVA+EGDS FGFS ME+ET+ RY LPV +++FNNGG+Y 
Sbjct: 429 GVMGIGMGYAIGASVTSGSP-VVAIEGDSAFGFSGMEIETICRYNLPVTIVIFNNGGIYR 488

Query: 527 GDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESFSARN 586
           GD     +++G     P+PT  +  A Y K+++AF G GY V T +EL+ AL+    +R 
Sbjct: 489 GD---GVDLSG--AGAPSPTDLLHHARYDKLMDAFRGVGYNVTTTDELRHALTTGIQSRK 548

Query: 587 PAVINVKIDPYAGAESGRLQHKN 607
           P +INV IDP AG ESG +   N
Sbjct: 549 PTIINVVIDPAAGTESGHITKLN 557

BLAST of Cp4.1LG02g13770 vs. NCBI nr
Match: XP_023524913.1 (2-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1156 bits (2990), Expect = 0.0
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. NCBI nr
Match: KAG7036762.1 (2-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1145 bits (2962), Expect = 0.0
Identity = 571/578 (98.79%), Postives = 576/578 (99.65%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGDV+ DLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVRADLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTV+ESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFIL+DICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILIDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSALSESFSAR+PAVINVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. NCBI nr
Match: XP_022948740.1 (2-hydroxyacyl-CoA lyase-like [Cucurbita moschata])

HSP 1 Score: 1145 bits (2962), Expect = 0.0
Identity = 571/578 (98.79%), Postives = 576/578 (99.65%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTV+ESEAE LLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAEGLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           P+KK VESTGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PMKKFVESTGIPFLPTPMGKGLLPDTHKLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSALSESFSAR+PAVINVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. NCBI nr
Match: XP_022998084.1 (2-hydroxyacyl-CoA lyase [Cucurbita maxima])

HSP 1 Score: 1144 bits (2959), Expect = 0.0
Identity = 569/578 (98.44%), Postives = 577/578 (99.83%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTV+E+EAERLLAAAEEFSHREVIHRVPNSQIEEAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTETEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDICKEEVE+RKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVEMRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAI+A+GSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAITALGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPV+VIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVIVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSALSESFSAR+PAVINVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. NCBI nr
Match: KAG6607061.1 (2-hydroxyacyl-CoA lyase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1143 bits (2956), Expect = 0.0
Identity = 570/578 (98.62%), Postives = 575/578 (99.48%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGDV+ DLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVRADLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTV+ESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFIL+DIC EEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILIDICMEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSALSESFSAR+PAVINVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. ExPASy TrEMBL
Match: A0A6J1GAR5 (2-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 SV=1)

HSP 1 Score: 1145 bits (2962), Expect = 0.0
Identity = 571/578 (98.79%), Postives = 576/578 (99.65%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTV+ESEAE LLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTESEAEGLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           P+KK VESTGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PMKKFVESTGIPFLPTPMGKGLLPDTHKLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSALSESFSAR+PAVINVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. ExPASy TrEMBL
Match: A0A6J1KDD5 (2-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1)

HSP 1 Score: 1144 bits (2959), Expect = 0.0
Identity = 569/578 (98.44%), Postives = 577/578 (99.83%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTV+E+EAERLLAAAEEFSHREVIHRVPNSQIEEAISLLK AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVTETEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKRAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDICKEEVE+RKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDICKEEVEMRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAI+A+GSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAITALGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPV+VIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVIVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSALSESFSAR+PAVINVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALSESFSARSPAVINVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. ExPASy TrEMBL
Match: A0A5A7VDZ6 (2-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2030G00330 PE=3 SV=1)

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 545/578 (94.29%), Postives = 566/578 (97.92%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSA  ++ DL+FHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQ+EAVKPFSK SVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QT+SESEAERLLAAAEEF++REVI RVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVE+TGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKA+DNVAKMEVQLA+DVVPFNF+TPMRIIRDAI AVGSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSAL+ESFSAR PAV+NVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. ExPASy TrEMBL
Match: A0A1S3B9H9 (2-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1)

HSP 1 Score: 1100 bits (2846), Expect = 0.0
Identity = 545/578 (94.29%), Postives = 566/578 (97.92%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSA  ++ DL+FHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAEALQNDLRFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAG+SNAM NAWPLVMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGISNAMVNAWPLVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQ+EAVKPFSK SVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQVEAVKPFSKISVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QT+SESEAERLLAAAEEF++REVI RVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG
Sbjct: 181 QTISESEAERLLAAAEEFANREVIPRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVE+TGIPFLPTPMGKGLLPDTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVETTGIPFLPTPMGKGLLPDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAK++LESINKEIKDDPFCLGKSHPWV A
Sbjct: 301 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKEVLESINKEIKDDPFCLGKSHPWVGA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKA+DNVAKMEVQLA+DVVPFNF+TPMRIIRDAI AVGSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKDNVAKMEVQLAKDVVPFNFLTPMRIIRDAILAVGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY+
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYE 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNN GVYGGDRRTPQEITGPYKHDPAPTSFVPSA+Y KMIEAFGGKGYYVE+P
Sbjct: 481 LPVVVIVFNNSGVYGGDRRTPQEITGPYKHDPAPTSFVPSASYDKMIEAFGGKGYYVESP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSAL+ESFSAR PAV+NVKIDPYAGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPYAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. ExPASy TrEMBL
Match: A0A6J1DW15 (2-hydroxyacyl-CoA lyase OS=Momordica charantia OX=3673 GN=LOC111024979 PE=3 SV=1)

HSP 1 Score: 1091 bits (2821), Expect = 0.0
Identity = 543/578 (93.94%), Postives = 563/578 (97.40%), Query Frame = 0

Query: 29  MGDSAGDVKTDLKFHGPIDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIA 88
           MGDSAGD++ DLKFHGPIDGNVLAAMALAR GVDRMFGVVGIPVTSLATRAVSLGIRFIA
Sbjct: 1   MGDSAGDLQNDLKFHGPIDGNVLAAMALARCGVDRMFGVVGIPVTSLATRAVSLGIRFIA 60

Query: 89  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRD 148
           FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWP+VMISGSCDQRD
Sbjct: 61  FHNEQSAGYAASAYGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPMVMISGSCDQRD 120

Query: 149 FGRGDFQELDQIEAVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLH 208
           FGRGDFQELDQIEAVKPFSKFSVKA DISEIPNCVA+VL+SA SGRPGGCYFDLPSDVLH
Sbjct: 121 FGRGDFQELDQIEAVKPFSKFSVKAKDISEIPNCVAQVLSSAASGRPGGCYFDLPSDVLH 180

Query: 209 QTVSESEAERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEG 268
           QTVSESEAERLL  AE+ + RE IHRVPNSQI+EAISLLK+AERPLIVFGKGAAIARAEG
Sbjct: 181 QTVSESEAERLLTEAEKSARREEIHRVPNSQIQEAISLLKNAERPLIVFGKGAAIARAEG 240

Query: 269 PLKKLVESTGIPFLPTPMGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGE 328
           PLKKLVESTGIPFLPTPMGKGLL DTH+LAATAARSLAIGKCDVALVVGARLNWLLHFGE
Sbjct: 241 PLKKLVESTGIPFLPTPMGKGLLSDTHELAATAARSLAIGKCDVALVVGARLNWLLHFGE 300

Query: 329 PPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEA 388
           PPKWSKDVKFILVDI +EEVELRKP LGLIGDAKQ+LESINKEIKDDPF LGKSHPWVEA
Sbjct: 301 PPKWSKDVKFILVDISEEEVELRKPRLGLIGDAKQVLESINKEIKDDPFSLGKSHPWVEA 360

Query: 389 ISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSV 448
           ISQKA++NV+KMEVQLARDVVPFNF+TPMRIIRDAISA GSP+PILVSEGANTMDVGRSV
Sbjct: 361 ISQKAKENVSKMEVQLARDVVPFNFLTPMRIIRDAISAQGSPAPILVSEGANTMDVGRSV 420

Query: 449 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 508
           LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ
Sbjct: 421 LVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQ 480

Query: 509 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 568
           LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP
Sbjct: 481 LPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETP 540

Query: 569 EELKSALSESFSARNPAVINVKIDPYAGAESGRLQHKN 606
           EELKSAL+ESFSAR PAV+NVKIDP+AGAESGRLQHKN
Sbjct: 541 EELKSALAESFSARKPAVVNVKIDPFAGAESGRLQHKN 578

BLAST of Cp4.1LG02g13770 vs. TAIR 10
Match: AT5G17380.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 914.8 bits (2363), Expect = 3.6e-266
Identity = 445/561 (79.32%), Postives = 500/561 (89.13%), Query Frame = 0

Query: 46  IDGNVLAAMALARFGVDRMFGVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYL 105
           IDGNVL A +L+  GV  MFGVVGIPVTSLA+RA++LGIRFIAFHNEQSAGYAASAYGYL
Sbjct: 12  IDGNVLVAKSLSHLGVTHMFGVVGIPVTSLASRAMALGIRFIAFHNEQSAGYAASAYGYL 71

Query: 106 TGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQIEAVKP 165
           TG+PG+LLTVSGPGCVHGLAG+SNA  N WP+VMISGSCDQRD GRGDFQELDQIEAVK 
Sbjct: 72  TGKPGILLTVSGPGCVHGLAGLSNAWVNTWPMVMISGSCDQRDVGRGDFQELDQIEAVKA 131

Query: 166 FSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLAAAEE 225
           FSK S KA D+ EIP+CV+RVL+ AVSGRPGGCY D+P+DVL Q +SESEA++L+   E 
Sbjct: 132 FSKLSEKAKDVREIPDCVSRVLDRAVSGRPGGCYLDIPTDVLRQKISESEADKLVDEVER 191

Query: 226 FSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVESTGIPFLPTP 285
               E I     S+IE A+SLL+ AERPLIVFGKGAA +RAE  LKKLVE TGIPFLPTP
Sbjct: 192 SRKEEPIRGSLRSEIESAVSLLRKAERPLIVFGKGAAYSRAEDELKKLVEITGIPFLPTP 251

Query: 286 MGKGLLPDTHQLAATAARSLAIGKCDVALVVGARLNWLLHFGEPPKWSKDVKFILVDICK 345
           MGKGLLPDTH+ +ATAARSLAIGKCDVALVVGARLNWLLHFGE PKW KDVKFILVD+ +
Sbjct: 252 MGKGLLPDTHEFSATAARSLAIGKCDVALVVGARLNWLLHFGESPKWDKDVKFILVDVSE 311

Query: 346 EEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAISQKARDNVAKMEVQLA 405
           EE+ELRKPHLG++GDAK ++  +N+EIKDDPFCLGKS+ WVE+IS+KA++N  KME+QLA
Sbjct: 312 EEIELRKPHLGIVGDAKTVIGLLNREIKDDPFCLGKSNSWVESISKKAKENGEKMEIQLA 371

Query: 406 RDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRSVLVQTEPRTRLDAGTWGT 465
           +DVVPFNF+TPMRIIRDAI AV  PSP++VSEGANTMDVGRSVLVQ EPRTRLDAGTWGT
Sbjct: 372 KDVVPFNFLTPMRIIRDAILAVEGPSPVVVSEGANTMDVGRSVLVQKEPRTRLDAGTWGT 431

Query: 466 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYQLPVVVIVFNNGGVYGGD 525
           MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRY L VV+IVFNNGGVYGGD
Sbjct: 432 MGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVETLVRYNLAVVIIVFNNGGVYGGD 491

Query: 526 RRTPQEITGPYKHDPAPTSFVPSANYHKMIEAFGGKGYYVETPEELKSALSESFSARNPA 585
           RR P+EI+GP+K DPAPTSFVP+A YHK+IEAFGGKGY VETP+ELKSAL+ESF+AR PA
Sbjct: 492 RRGPEEISGPHKEDPAPTSFVPNAGYHKLIEAFGGKGYIVETPDELKSALAESFAARKPA 551

Query: 586 VINVKIDPYAGAESGRLQHKN 607
           V+NV IDP+AGAESGRLQHKN
Sbjct: 552 VVNVIIDPFAGAESGRLQHKN 572

BLAST of Cp4.1LG02g13770 vs. TAIR 10
Match: AT3G48560.1 (chlorsulfuron/imidazolinone resistant 1 )

HSP 1 Score: 174.5 bits (441), Expect = 2.6e-43
Identity = 135/495 (27.27%), Postives = 227/495 (45.86%), Query Frame = 0

Query: 45  PIDGNVLAAMALARFGVDRMF---GVVGIPVTSLATRAVSLGIRFIAFHNEQSAGYAASA 104
           P  G  +   AL R GV+ +F   G   + +    TR+ S  IR +   +EQ   +AA  
Sbjct: 96  PRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSS--IRNVLPRHEQGGVFAAEG 155

Query: 105 YGYLTGRPGVLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRGDFQELDQIE 164
           Y   +G+PG+ +  SGPG  + ++G+++A+ ++ PLV I+G   +R  G   FQE   +E
Sbjct: 156 YARSSGKPGICIATSGPGATNLVSGLADALLDSVPLVAITGQVPRRMIGTDAFQETPIVE 215

Query: 165 AVKPFSKFSVKATDISEIPNCVARVLNSAVSGRPGGCYFDLPSDVLHQTVSESEAERLLA 224
             +  +K +    D+ +IP  +      A SGRPG    D+P D+  Q    +  + +  
Sbjct: 216 VTRSITKHNYLVMDVEDIPRIIEEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRL 275

Query: 225 AAEEFSHREVIHRVP----NSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVEST 284
                     + R+P    +S +E+ + L+  +++P++  G G   +  E  L + VE T
Sbjct: 276 PG-------YMSRMPKPPEDSHLEQIVRLISESKKPVLYVGGGCLNSSDE--LGRFVELT 335

Query: 285 GIPFLPTPMGKGLLPDTHQLA-------ATAARSLAIGKCDVALVVGARLNWLLHFGEPP 344
           GIP   T MG G  P   +L+        T   + A+   D+ L  G R +  +  G+  
Sbjct: 336 GIPVASTLMGLGSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRV-TGKLE 395

Query: 345 KWSKDVKFILVDICKEEVELRK-PHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVEAI 404
            ++   K + +DI   E+   K PH+ + GD K  L+ +NK +++             A 
Sbjct: 396 AFASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLEN------------RAE 455

Query: 405 SQKARDNVAKMEVQLARDVVPFNFMT------PMRIIRDAISAVGSPSPILVSEGANTMD 464
             K    V + E+ + +   P +F T      P   I+         + I    G + M 
Sbjct: 456 ELKLDFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGVGQHQMW 515

Query: 465 VGRSVLVQTEPRTRLDAGTWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVET 519
             +    + +PR  L +G  G MG GL   I A+VA+PD +VV ++GD  F  +  E+ T
Sbjct: 516 AAQFYNYK-KPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDIDGDGSFIMNVQELAT 565

BLAST of Cp4.1LG02g13770 vs. TAIR 10
Match: AT4G33070.1 (Thiamine pyrophosphate dependent pyruvate decarboxylase family protein )

HSP 1 Score: 66.6 bits (161), Expect = 7.7e-11
Identity = 119/574 (20.73%), Postives = 218/574 (37.98%), Query Frame = 0

Query: 53  AMALARFGVDRMFGVVG-IPVTSLATRAVSLGIRFIAFHNEQSAGYAASAYGYLTGRPG- 112
           A  L + GV  +F V G   +T L        +  I   NE +AGYAA  Y    G    
Sbjct: 52  ARRLVQAGVTDVFSVPGDFNLTLLDHLMAEPDLNLIGCCNELNAGYAADGYARSRGVGAC 111

Query: 113 -VLLTVSGPGCVHGLAGVSNAMANAWPLVMISGSCDQRDFGRG----------DFQELDQ 172
            V  TV G   ++ +AG   A +   PL+ I G  +  D+G            DF +  +
Sbjct: 112 VVTFTVGGLSVLNAIAG---AYSENLPLICIVGGPNSNDYGTNRILHHTIGLPDFSQ--E 171

Query: 173 IEAVKPFSKFSVKATDISEIPNCVARVLNSAV-SGRPGGCYFDLPSD---VLHQTVSESE 232
           +   +  + +     ++ +    + + +++A+   +P   Y  +  +   + H T S   
Sbjct: 172 LRCFQTVTCYQAVVNNLDDAHEQIDKAISTALKESKP--VYISVSCNLAAIPHHTFSRDP 231

Query: 233 AERLLAAAEEFSHREVIHRVPNSQIEEAISLLKHAERPLIVFGKGAAIARAEGPLKKLVE 292
                + A   S++  +     + +E  +  L  A +P++V G    +A+A     +L +
Sbjct: 232 VP--FSLAPRLSNKMGL----EAAVEATLEFLNKAVKPVMVGGPKLRVAKACDAFVELAD 291

Query: 293 STGIPFLPTPMGKGLLPDTHQ--------LAATAARSLAIGKCDVALVVGARLNWLLHFG 352
           ++G      P  KG +P+ H           +T   S  +   D  +  G   N     G
Sbjct: 292 ASGYALAMMPSAKGFVPEHHPHFIGTYWGAVSTPFCSEIVESADAYIFAGPIFNDYSSVG 351

Query: 353 EPPKWSKDVKFILVDICKEEVELRKPHLGLIGDAKQILESINKEIKDDPFCLGKSHPWVE 412
                 K+ K I+V   +  V    P  G I         ++K +K +       H    
Sbjct: 352 YSLLLKKE-KAIVVQPDRITV-ANGPTFGCI-LMSDFFRELSKRVKRNETAYENYH---- 411

Query: 413 AISQKARDNVAKMEVQLARDVVPFNFMTPMRIIRDAISAVGSPSPILVSEGANTMDVGRS 472
                 R  V + +        P    T  + I+  +S          SE A   + G S
Sbjct: 412 ------RIFVPEGKPLKCESREPLRVNTMFQHIQKMLS----------SETAVIAETGDS 471

Query: 473 VLVQTEPRTRLDAG-----TWGTMGVGLGYCIAAAVASPDRLVVAVEGDSGFGFSAMEVE 532
                + +     G      +G++G  +G  +  A ASP++ V+A  GD  F  +  ++ 
Sbjct: 472 WFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQASPEKRVLAFIGDGSFQVTVQDIS 531

Query: 533 TLVRYQLPVVVIVFNNGGVYGGDRRTPQEITGPYKHDPAPTSFVPSANYHKMIEAF-GGK 592
           T++R     ++ + NNGG          EI     HD  P + + + NY  +++A   G+
Sbjct: 532 TMLRNGQKTIIFLINNGGY-----TIEVEI-----HD-GPYNVIKNWNYTGLVDAIHNGE 578

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LF465.0e-26579.322-hydroxyacyl-CoA lyase OS=Arabidopsis thaliana OX=3702 GN=HACL PE=1 SV=1[more]
Q0JMH04.2e-25676.112-hydroxyacyl-CoA lyase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0505400... [more]
Q8CHM72.0e-12843.912-hydroxyacyl-CoA lyase 1 OS=Rattus norvegicus OX=10116 GN=Hacl1 PE=1 SV=1[more]
P0AFI15.8e-12843.69Oxalyl-CoA decarboxylase OS=Escherichia coli O157:H7 OX=83334 GN=oxc PE=3 SV=1[more]
P0AFI05.8e-12843.69Oxalyl-CoA decarboxylase OS=Escherichia coli (strain K12) OX=83333 GN=oxc PE=1 S... [more]
Match NameE-valueIdentityDescription
XP_023524913.10.0100.002-hydroxyacyl-CoA lyase-like [Cucurbita pepo subsp. pepo][more]
KAG7036762.10.098.792-hydroxyacyl-CoA lyase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022948740.10.098.792-hydroxyacyl-CoA lyase-like [Cucurbita moschata][more]
XP_022998084.10.098.442-hydroxyacyl-CoA lyase [Cucurbita maxima][more]
KAG6607061.10.098.622-hydroxyacyl-CoA lyase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1GAR50.098.792-hydroxyacyl-CoA lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111452318 PE=3 ... [more]
A0A6J1KDD50.098.442-hydroxyacyl-CoA lyase OS=Cucurbita maxima OX=3661 GN=LOC111492838 PE=3 SV=1[more]
A0A5A7VDZ60.094.292-hydroxyacyl-CoA lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A1S3B9H90.094.292-hydroxyacyl-CoA lyase OS=Cucumis melo OX=3656 GN=LOC103487508 PE=3 SV=1[more]
A0A6J1DW150.093.942-hydroxyacyl-CoA lyase OS=Momordica charantia OX=3673 GN=LOC111024979 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G17380.13.6e-26679.32Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
AT3G48560.12.6e-4327.27chlorsulfuron/imidazolinone resistant 1 [more]
AT4G33070.17.7e-1120.73Thiamine pyrophosphate dependent pyruvate decarboxylase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011766Thiamine pyrophosphate enzyme, C-terminal TPP-bindingPFAMPF02775TPP_enzyme_Ccoord: 440..589
e-value: 1.8E-28
score: 99.3
NoneNo IPR availableGENE3D3.40.50.970coord: 46..227
e-value: 2.5E-43
score: 149.6
NoneNo IPR availableGENE3D3.40.50.1220coord: 236..390
e-value: 2.4E-37
score: 130.0
NoneNo IPR availableGENE3D3.40.50.970coord: 392..606
e-value: 1.0E-64
score: 219.5
NoneNo IPR availablePANTHERPTHR43710:SF22-HYDROXYACYL-COA LYASE 1coord: 46..606
NoneNo IPR availableCDDcd07035TPP_PYR_POX_likecoord: 55..203
e-value: 4.87842E-46
score: 157.693
NoneNo IPR availableCDDcd02004TPP_BZL_OCoD_HPCLcoord: 417..592
e-value: 1.06569E-81
score: 252.453
IPR012000Thiamine pyrophosphate enzyme, central domainPFAMPF00205TPP_enzyme_Mcoord: 240..367
e-value: 3.0E-32
score: 111.2
IPR012001Thiamine pyrophosphate enzyme, N-terminal TPP-binding domainPFAMPF02776TPP_enzyme_Ncoord: 53..213
e-value: 2.4E-40
score: 137.9
IPR045025TPP-binding domain containing protein HACL1-likePANTHERPTHR437102-HYDROXYACYL-COA LYASEcoord: 46..606
IPR000399TPP-binding enzyme, conserved sitePROSITEPS00187TPP_ENZYMEScoord: 474..493
IPR029035DHS-like NAD/FAD-binding domain superfamilySUPERFAMILY52467DHS-like NAD/FAD-binding domaincoord: 237..392
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 419..595
IPR029061Thiamin diphosphate-binding foldSUPERFAMILY52518Thiamin diphosphate-binding fold (THDP-binding)coord: 47..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g13770.1Cp4.1LG02g13770.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0001561 fatty acid alpha-oxidation
cellular_component GO:0005777 peroxisome
molecular_function GO:0016829 lyase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0030976 thiamine pyrophosphate binding
molecular_function GO:0003824 catalytic activity