Cp4.1LG02g13400 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g13400
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein kinase domain-containing protein
LocationCp4.1LG02: 11091006 .. 11093918 (-)
RNA-Seq ExpressionCp4.1LG02g13400
SyntenyCp4.1LG02g13400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTGGAGTGATCAGTGATGAGCTCTCTTATGACTTTTCGCCGCCTCCATGGATTGTGGACGTGGTGAAATCAAATGGTGGAGAATCGTATCAAATTTCTGCACCTCAAAACCTTATCTGTTCGATTTCTTCTTCTGATTTGACCAGGTAAGAGCTTCCAAAAAGATGAGATATCAAAAGGGTCATCAAAAAAGGTTGTGCGGGGCCGCGGACTGAGCGCCATTAGTGCAAATTTCTCCACCTTTGCGCGCACTGGACCCCCTTTCAACCATCTCCCACTGCTCCATTCTTTATTCATTTCTTCTATCGGACACTGTTCCTCAATTTTCCCAGTCAAAGTCTCTCAACCCTTTTTCTCCTCCCTTTTCTCTCTAAATAGGCCCTTCCAAGATTCCTTCCCAATTCGATTCCACCCTTTTGAATATTTGTTTCCCCTCTTATATCTGCCCTCTCTACTTCGTTTGTTACTGAGATCTAAGTCTGTGGGGTGAACTGGTAAGAAATTTCTCCTTCCCCTGTTTGTGGGGTTTTAGAATTTTGTTGTTATAACTTGAATTCTGAAGTTGTGATTGCATGCAAGTGTGTTCTTGAGGTTTTTTTTTTACTCATTTTAAGTGATACCCATCTTTCTTTGCTTGTAAATCTACATTTTAGAACTTAGATCTGCAAGTTTTGTCTCTTTCTTTTGGTTGCTGTTGAAATTTCTTGCTGTAAGCCGCTGAATTTTGTGATACGCAGTAAGAAAACTGGTCAGGCTGTGAGATTTCGGGCCAGTCCCATGGGGATTTGCTTAAGCGCCAGAATTAAAGCTGTTAGCACATGCAGTACTGGTACAGGTAGAGCTCGGAAGCCATGGAAATGGATATCTTGCCCATTGTGTTTACTTTTAGTTGTTCATTTATTGGGAAAAGAAAGGGGGAAAAACATGTTTTCATGAATTTACTTGGCAAATGGTTGGAGATTCATTCTCCAGGCTTAGCCATTTGTTGTCTGTTGTATGAACTTGAATGATTAAGATCTGTTAGTCAACTCATTAGCTCATAAAATGTCATGTTTCTAAATTTAGCTACTATGGGATTGTAATGATGTTGAGTTGAAGATTCTCTGATGAACTTCAATCTGGTATTAGAATGTTCTTACGCGAATCGAAATTGGAGAATCGTGTTCTTCTGACACTCAAAATTGTATATGCAATGCAACTTGATTTGGCCATGTTCAGATATGGATATCTTACATTTAAGTTATGCAGGAGGAACTTTAAATGGCGTTAGTAAGGATGCAATCGATATCTCTAGTTCGAGTAGCAACAGCAAATTCTCATCGACATCAATATCTCGGAGTGAGGATGAGATCCTGCAGTCCTCAAATTTGAAGAGTTTTAGTTTCTCGGAACTTAAGCTAGCCACTAGGAACTTTCGCCCCGATAGTCTGTTGGGGGAAGGTGGGTTCGGTTCAGTTTATAAAGGTTGGATTCATGAGGAATCATCTTCTGCTGCCAAGCCAGGGTCTGGCACGGTTGTTGCGGTGAAAAGGCTTAACCTCGATGGTCTCCAAGGTCACAAGGAGTGGCTGGTAAGTCAATGTCAGGCTGCTTTATCTTTGGCTTCAACAAAGTAAGTTTCCAATCCGCTTACCGTTTAGTTTCTTCGATACTGTAGGCTGAAGTCAATTTCCTGGGCCAACTTCATCATTCTCATCTAGTGAGACTGATAGGCTATTGTTTGGAAGACGAACATCGGTTGCTGGTGTATGAGTTCCTTACCAGAGGCAGCTTGGAGAACCATTTGTTTAGGAGTGAGTCGTCATCTCCTACCCCAATTAGGCAAAAAGGATCCGTTGCTTAGGAATGTCGATTTATTCATATTATTGCAACTTTTTCTTCAGGGGGGCTGTATTTTCAACCTCTTTCGTGGAGCCTTCGCTTGAAGATCGCTCTTGGCGCTGCAATGGGGCTAGCTTTCCTTCACAGCGACGAAAACAAAGTTATCTATCGCGATTTCAAGACATCAAATATACTCCTCGATTCAGTAAGTATAGTTGCAGTGTGGCACGGTTTCGATTTCAATTATAGCCTGTATATCTGAAGACTCTATTCTATGATGTGGTTACAGGACTACAATGCAAAGCTCTCCGACTTTGGCCTTGCTAAGGATGGACCGACGGGCGACAAAAGTCACGTCTCCACCAGAATTATGGGGACGTATGGATATGCAGCTCCTGAGTATCTGGCCACAGGTAATACTCGTTCTAACATCAAAAAGAATTTCTTTTAGTGATATCGTCTGTTGATCTCTTATTTCATTCGAATGCGACTACTAGGTCATCTTAGTGCGAAGAGTGATGTGTATAGTTTCGGGGTAGTTCTGTTGGAGATTTTATCTGGTAGAAGAGCAATAGACAAGAATCGACCACCAGGGGAACAGAATCTGGTCGAGTGGGCCAAACCCTTTCTTGCTAATAAACGCAAAACTTATCGTTTACTTGATGCACGAACCGAACGCAACTACTCCATGGAATCAGCTAGTAGAGTAGCAATTCTTGCATCACGTTGCCTGTCTGCAGAACCGAAGTTCAGGCCGAGTATGAGAGACGTCGTAACGACATTAAAGGATCTCCAGGATTTAACAGGAAATGGAGGTAATGGGAATAGAAGATGCAGGAGTAGTAAGCAAGGCTCACACAGTGGTAATGCACAACACAAGGAAGTGTCTGCATATCCTAGACCATCGGCATCAGCTCTGTTTACATAAGCCGGGGTCGAGGGTCGAGGGGATCGGATTGTATAGTGTTCTTATTTCATTGCAACTAATGTATACTTACATAGCTATTTTGTGATATTCAGTTTTGTAATACTTTTCTTCAATCACTTGAGATCTATGTTAATGAAAGATGATTTCATCAGTTCTTATAAGAA

mRNA sequence

GTTGGAGTGATCAGTGATGAGCTCTCTTATGACTTTTCGCCGCCTCCATGGATTGTGGACGTGGTGAAATCAAATGGTGGAGAATCGTATCAAATTTCTGCACCTCAAAACCTTATCTGTTCGATTTCTTCTTCTGATTTGACCAGTAAGAAAACTGGTCAGGCTGTGAGATTTCGGGCCAGTCCCATGGGGATTTGCTTAAGCGCCAGAATTAAAGCTGTTAGCACATGCAGTACTGGTACAGATATGGATATCTTACATTTAAGTTATGCAGGAGGAACTTTAAATGGCGTTAGTAAGGATGCAATCGATATCTCTAGTTCGAGTAGCAACAGCAAATTCTCATCGACATCAATATCTCGGAGTGAGGATGAGATCCTGCAGTCCTCAAATTTGAAGAGTTTTAGTTTCTCGGAACTTAAGCTAGCCACTAGGAACTTTCGCCCCGATAGTCTGTTGGGGGAAGGTGGGTTCGGTTCAGTTTATAAAGGTTGGATTCATGAGGAATCATCTTCTGCTGCCAAGCCAGGGTCTGGCACGGTTGTTGCGGTGAAAAGGCTTAACCTCGATGGTCTCCAAGGTCACAAGGAGTGGCTGGTAAGTCAATGTCAGGCTGCTTTATCTTTGGCTTCAACAAAGGGGGCTGTATTTTCAACCTCTTTCGTGGAGCCTTCGCTTGAAGATCGCTCTTGGCGCTGCAATGGGGCTAGCTTTCCTTCACAGCGACGAAAACAAAGTTATCTATCGCGATTTCAAGACATCAAATATACTCCTCGATTCAGTCATCTTAGTGCGAAGAGTGATGTGTATAGTTTCGGGGTAGTTCTGTTGGAGATTTTATCTGGTAGAAGAGCAATAGACAAGAATCGACCACCAGGGGAACAGAATCTGGTCGAGTGGGCCAAACCCTTTCTTGCTAATAAACGCAAAACTTATCGTTTACTTGATGCACGAACCGAACGCAACTACTCCATGGAATCAGCTAGTAGAGTAGCAATTCTTGCATCACGTTGCCTGTCTGCAGAACCGAAGTTCAGGCCGAGTATGAGAGACGTCGTAACGACATTAAAGGATCTCCAGGATTTAACAGGAAATGGAGGTAATGGGAATAGAAGATGCAGGAGTAGTAAGCAAGGCTCACACAGTGGTAATGCACAACACAAGGAAGTGTCTGCATATCCTAGACCATCGGCATCAGCTCTGTTTACATAAGCCGGGGTCGAGGGTCGAGGGGATCGGATTGTATAGTGTTCTTATTTCATTGCAACTAATGTATACTTACATAGCTATTTTGTGATATTCAGTTTTGTAATACTTTTCTTCAATCACTTGAGATCTATGTTAATGAAAGATGATTTCATCAGTTCTTATAAGAA

Coding sequence (CDS)

GTTGGAGTGATCAGTGATGAGCTCTCTTATGACTTTTCGCCGCCTCCATGGATTGTGGACGTGGTGAAATCAAATGGTGGAGAATCGTATCAAATTTCTGCACCTCAAAACCTTATCTGTTCGATTTCTTCTTCTGATTTGACCAGTAAGAAAACTGGTCAGGCTGTGAGATTTCGGGCCAGTCCCATGGGGATTTGCTTAAGCGCCAGAATTAAAGCTGTTAGCACATGCAGTACTGGTACAGATATGGATATCTTACATTTAAGTTATGCAGGAGGAACTTTAAATGGCGTTAGTAAGGATGCAATCGATATCTCTAGTTCGAGTAGCAACAGCAAATTCTCATCGACATCAATATCTCGGAGTGAGGATGAGATCCTGCAGTCCTCAAATTTGAAGAGTTTTAGTTTCTCGGAACTTAAGCTAGCCACTAGGAACTTTCGCCCCGATAGTCTGTTGGGGGAAGGTGGGTTCGGTTCAGTTTATAAAGGTTGGATTCATGAGGAATCATCTTCTGCTGCCAAGCCAGGGTCTGGCACGGTTGTTGCGGTGAAAAGGCTTAACCTCGATGGTCTCCAAGGTCACAAGGAGTGGCTGGTAAGTCAATGTCAGGCTGCTTTATCTTTGGCTTCAACAAAGGGGGCTGTATTTTCAACCTCTTTCGTGGAGCCTTCGCTTGAAGATCGCTCTTGGCGCTGCAATGGGGCTAGCTTTCCTTCACAGCGACGAAAACAAAGTTATCTATCGCGATTTCAAGACATCAAATATACTCCTCGATTCAGTCATCTTAGTGCGAAGAGTGATGTGTATAGTTTCGGGGTAGTTCTGTTGGAGATTTTATCTGGTAGAAGAGCAATAGACAAGAATCGACCACCAGGGGAACAGAATCTGGTCGAGTGGGCCAAACCCTTTCTTGCTAATAAACGCAAAACTTATCGTTTACTTGATGCACGAACCGAACGCAACTACTCCATGGAATCAGCTAGTAGAGTAGCAATTCTTGCATCACGTTGCCTGTCTGCAGAACCGAAGTTCAGGCCGAGTATGAGAGACGTCGTAACGACATTAAAGGATCTCCAGGATTTAACAGGAAATGGAGGTAATGGGAATAGAAGATGCAGGAGTAGTAAGCAAGGCTCACACAGTGGTAATGCACAACACAAGGAAGTGTCTGCATATCCTAGACCATCGGCATCAGCTCTGTTTACATAA

Protein sequence

VGVISDELSYDFSPPPWIVDVVKSNGGESYQISAPQNLICSISSSDLTSKKTGQAVRFRASPMGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVVAVKRLNLDGLQGHKEWLVSQCQAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQSYLSRFQDIKYTPRFSHLSAKSDVYSFGVVLLEILSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRPSMRDVVTTLKDLQDLTGNGGNGNRRCRSSKQGSHSGNAQHKEVSAYPRPSASALFT
Homology
BLAST of Cp4.1LG02g13400 vs. ExPASy Swiss-Prot
Match: P46573 (Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 GN=PBL10 PE=1 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 3.3e-63
Identity = 176/430 (40.93%), Postives = 228/430 (53.02%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSA+IKAVS                G +   +S +A D S  S +S  S  +  R+
Sbjct: 1   MGICLSAQIKAVS---------------PGASPKYMSSEAND-SLGSKSSSVSIRTNPRT 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           E EILQS NLKSF+F+ELK ATRNFRPDS+LGEGGFGSV+KGWI E++ +A+KPG+G V+
Sbjct: 61  EGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVI 120

Query: 183 AVKRLNLDGLQGHKEW-----------------LVSQC---------------------- 242
           AVK+LN DG QGH+EW                 L+  C                      
Sbjct: 121 AVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHL 180

Query: 243 --------------QAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQSYLS 302
                         +  ++L + KG  F  +  E S+  R ++ +     S+   +  LS
Sbjct: 181 FRRGSYFQPLSWTLRLKVALGAAKGLAFLHN-AETSVIYRDFKTSNILLDSEYNAK--LS 240

Query: 303 RFQDIKYTP-----------------------RFSHLSAKSDVYSFGVVLLEILSGRRAI 362
            F   K  P                          HL+ KSDVYS+GVVLLE+LSGRRA+
Sbjct: 241 DFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAV 300

Query: 363 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFR 403
           DKNRPPGEQ LVEWA+P LANKRK +R++D R +  YSME A +VA LA RCL+ E K R
Sbjct: 301 DKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLR 360

BLAST of Cp4.1LG02g13400 vs. ExPASy Swiss-Prot
Match: Q06548 (Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN=PBL9 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 3.1e-61
Identity = 173/432 (40.05%), Postives = 224/432 (51.85%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSI--- 122
           MGICLSA++KA S+ ++                     DA DI   S  SK SS S+   
Sbjct: 1   MGICLSAQVKAESSGAS------------------TKYDAKDI--GSLGSKASSVSVRPS 60

Query: 123 SRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSG 182
            R+E EILQS NLKSFSF+ELK ATRNFRPDS+LGEGGFG V+KGWI E+S +A++PG+G
Sbjct: 61  PRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTG 120

Query: 183 TVVAVKRLNLDGLQGHKEWLVS-------------------------------------- 242
            V+AVK+LN DG QGH+EWL                                        
Sbjct: 121 LVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLE 180

Query: 243 ---------------QCQAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQS 302
                          + +  ++L + KG  F  S  E  +  R ++ +     S+   + 
Sbjct: 181 NHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHS-SETRVIYRDFKTSNILLDSEYNAK- 240

Query: 303 YLSRFQDIKYTP-----------------------RFSHLSAKSDVYSFGVVLLEILSGR 362
            LS F   K  P                          HL+ KSDVYSFGVVLLE+LSGR
Sbjct: 241 -LSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGR 300

Query: 363 RAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEP 403
           RA+DKNRP GE+NLVEWAKP+L NKRK +R++D R +  YSME A +VA L+ RCL+ E 
Sbjct: 301 RAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEI 360

BLAST of Cp4.1LG02g13400 vs. ExPASy Swiss-Prot
Match: Q65XV8 (Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK176 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 3.3e-55
Identity = 154/403 (38.21%), Postives = 209/403 (51.86%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSI--- 122
           MG C  A+I + S C + +       S +GGT           S  +SNS  S+ S+   
Sbjct: 1   MGNCWGAKISSESPCRSAS-------SPSGGT-----------SKYASNSSVSAASVPPT 60

Query: 123 SRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSG 182
            RSEDEIL+++N+K+F+F+EL+ ATRNFRPDS+LGEGGFGSV+KGWI E++ +  KPG+G
Sbjct: 61  PRSEDEILEAANVKAFAFNELRTATRNFRPDSVLGEGGFGSVFKGWIDEKTLAPTKPGTG 120

Query: 183 TVVAVKRLNLDGLQGHKEW-----------------LVSQC------------------- 242
            V+AVK+LN +G QGH+EW                 LV  C                   
Sbjct: 121 MVIAVKKLNQEGHQGHREWLAEVNYLGQLSHPYLVRLVGYCVEDEQRLLVYEFMPRGSLE 180

Query: 243 -----------------QAALSLASTKGAV-------------FSTSFV------EPSLE 302
                            +  ++L + KG               F TS V      +  L 
Sbjct: 181 NHLFRRSTHFQPLSWNLRMKIALGAAKGLAFLHSDKVKVIYRDFKTSNVLLDANYDAKLS 240

Query: 303 DRSWRCNG----ASFPSQRRKQSYLSRFQDIKYTPRFSHLSAKSDVYSFGVVLLEILSGR 362
           D     +G     S  S R   +Y     +   T    HL+ KSDVYSFGVVLLE+LSGR
Sbjct: 241 DFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLAT---GHLTTKSDVYSFGVVLLEMLSGR 300

Query: 363 RAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEP 387
           RA+DKNRP GE NLVEWA+P+L +KR+ +R+LDAR    YS+  A + A LA +C+S E 
Sbjct: 301 RALDKNRPTGEHNLVEWARPYLMSKRRIFRILDARLGGQYSLAKAQKAATLALQCISVEA 360

BLAST of Cp4.1LG02g13400 vs. ExPASy Swiss-Prot
Match: P43293 (Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 GN=PBL11 PE=1 SV=2)

HSP 1 Score: 209.1 bits (531), Expect = 9.0e-53
Identity = 142/370 (38.38%), Postives = 192/370 (51.89%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MG C S RIK     ST      L            S+D    S  SS + FS   + R+
Sbjct: 1   MGGCFSNRIKTDIASSTWLSSKFL------------SRDG---SKGSSTASFS--YMPRT 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           E EILQ++NLK+FS SELK ATRNFRPDS++GEGGFG V+KGWI E S + +KPG+G V+
Sbjct: 61  EGEILQNANLKNFSLSELKSATRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVI 120

Query: 183 AVKRLNLDGLQGHKEW-----------------LVSQC-----QAALSLASTKGAVFSTS 242
           AVKRLN +G QGH+EW                 L+  C     +  +    T+G++ +  
Sbjct: 121 AVKRLNQEGFQGHREWLAEINYLGQLDHPNLVKLIGYCLEEEHRLLVYEFMTRGSLENHL 180

Query: 243 FVEPSL-EDRSWR---------CNGASFPSQRRKQSYLSRFQ------DIKYTPRFS--- 302
           F   +  +  SW            G +F    + Q     F+      D  Y  + S   
Sbjct: 181 FRRGTFYQPLSWNTRVRMALGAARGLAFLHNAQPQVIYRDFKASNILLDSNYNAKLSDFG 240

Query: 303 --------------------------------HLSAKSDVYSFGVVLLEILSGRRAIDKN 360
                                           HLS KSDVYSFGVVLLE+LSGRRAIDKN
Sbjct: 241 LARDGPMGDNSHVSTRVMGTQGYAAPEYLATGHLSVKSDVYSFGVVLLELLSGRRAIDKN 300

BLAST of Cp4.1LG02g13400 vs. ExPASy Swiss-Prot
Match: O48814 (Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=1 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 7.6e-52
Identity = 148/407 (36.36%), Postives = 201/407 (49.39%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MG C S+R+KA          DI H        NG S D   +S SS  S  +  +  ++
Sbjct: 1   MGSCFSSRVKA----------DIFH--------NGKSSDLYGLSLSSRKSSSTVAAAQKT 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           E EIL S+ +KSF+F+ELKLATRNFRPDS++GEGGFG V+KGW+ E + +  KPG+G V+
Sbjct: 61  EGEILSSTPVKSFTFNELKLATRNFRPDSVIGEGGFGCVFKGWLDESTLTPTKPGTGLVI 120

Query: 183 AVKRLNLDGLQGHKEW-----------------LVSQC---------------------- 242
           AVK+LN +G QGH+EW                 L+  C                      
Sbjct: 121 AVKKLNQEGFQGHREWLTEINYLGQLSHPNLVKLIGYCLEDEHRLLVYEFMQKGSLENHL 180

Query: 243 --------------QAALSLASTKGAVFSTS----------FVEPSLEDRSWRCNGASFP 302
                         +  ++L + KG  F  S               L D  +    + F 
Sbjct: 181 FRRGAYFKPLPWFLRVNVALDAAKGLAFLHSDPVKVIYRDIKASNILLDADYNAKLSDFG 240

Query: 303 SQR----RKQSYLSRFQDIKY---TPRF---SHLSAKSDVYSFGVVLLEILSGRRAIDKN 362
             R       SY+S      Y    P +    HL+A+SDVYSFGV+LLEILSG+RA+D N
Sbjct: 241 LARDGPMGDLSYVSTRVMGTYGYAAPEYMSSGHLNARSDVYSFGVLLLEILSGKRALDHN 300

Query: 363 RPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRPSM 397
           RP  E+NLV+WA+P+L +KRK   ++D R +  Y  E A R+A +A +CLS EPK RP+M
Sbjct: 301 RPAKEENLVDWARPYLTSKRKVLLIVDNRLDTQYLPEEAVRMASVAVQCLSFEPKSRPTM 360

BLAST of Cp4.1LG02g13400 vs. NCBI nr
Match: KAG6607247.1 (putative serine/threonine-protein kinase PBL10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 483 bits (1243), Expect = 1.65e-165
Identity = 292/430 (67.91%), Postives = 303/430 (70.47%), Query Frame = 0

Query: 50  KKTGQAVRFRASPMGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSS 109
           KKTGQAVRFRASPMGICLSARIKAVSTCSTGT          GGTLNGVSKDAIDISSSS
Sbjct: 68  KKTGQAVRFRASPMGICLSARIKAVSTCSTGT----------GGTLNGVSKDAIDISSSS 127

Query: 110 SNSKFSSTSISRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEE 169
           SNSKFSSTSISRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEE
Sbjct: 128 SNSKFSSTSISRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEE 187

Query: 170 SSSAAKPGSGTVVAVKRLNLDGLQGHKEWL-----------------VSQCQA------- 229
           SSSAAKPGSGTVVAVKRLNLDGLQGHKEWL                 +  C         
Sbjct: 188 SSSAAKPGSGTVVAVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLV 247

Query: 230 -------------------------ALSLASTKGAVFSTSFV---EPSLEDRSWRCNGAS 289
                                    +L L    GA    +F+   E  +  R ++ +   
Sbjct: 248 YEFLTRGSLENHLFRRGLYFQPLSWSLRLKIALGAAMGLAFLHSDENKVIYRDFKTSNIL 307

Query: 290 FPSQRRKQSYLSRFQDIK--------------------YTPRF---SHLSAKSDVYSFGV 349
             S     + LS F   K                      P +    HLSAKSDVYSFGV
Sbjct: 308 LDSD--YNAKLSDFGLAKDGLTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYSFGV 367

Query: 350 VLLEILSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAIL 403
           VLLEILSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAIL
Sbjct: 368 VLLEILSGRRAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAIL 427

BLAST of Cp4.1LG02g13400 vs. NCBI nr
Match: XP_023523063.1 (probable serine/threonine-protein kinase PBL10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 472 bits (1215), Expect = 1.79e-162
Identity = 283/416 (68.03%), Postives = 292/416 (70.19%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSARIKAVSTCSTGT          GGTLNGVSKDAIDISSSSSNSKFSSTSISRS
Sbjct: 1   MGICLSARIKAVSTCSTGT----------GGTLNGVSKDAIDISSSSSNSKFSSTSISRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQCQA-------------------- 242
           AVKRLNLDGLQGHKEWL                 +  C                      
Sbjct: 121 AVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLTRGSLENHL 180

Query: 243 ------------ALSLASTKGAVFSTSFV---EPSLEDRSWRCNGASFPSQRRKQSYLSR 302
                       +L L    GA    +F+   E  +  R ++ +     S     + LS 
Sbjct: 181 FRRGLYFQPLSWSLRLKIALGAAMGLAFLHSDENKVIYRDFKTSNILLDSD--YNAKLSD 240

Query: 303 FQDIKYTPR-----------------------FSHLSAKSDVYSFGVVLLEILSGRRAID 362
           F   K  P                          HLSAKSDVYSFGVVLLEILSGRRAID
Sbjct: 241 FGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYSFGVVLLEILSGRRAID 300

Query: 363 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 403
           KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP
Sbjct: 301 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 360

BLAST of Cp4.1LG02g13400 vs. NCBI nr
Match: KAG7036931.1 (putative serine/threonine-protein kinase PBL10 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 459 bits (1181), Expect = 2.68e-157
Identity = 278/417 (66.67%), Postives = 289/417 (69.30%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSARIKAVSTCSTGT          GGTLNGVSKDAIDISSSSSNSKFSSTSISRS
Sbjct: 1   MGICLSARIKAVSTCSTGT----------GGTLNGVSKDAIDISSSSSNSKFSSTSISRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQCQA-------------------- 242
           AVKRLNLDGLQGHKEWL                 +  C                      
Sbjct: 121 AVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLTRGSLENHL 180

Query: 243 ------------ALSLASTKGAVFSTSFV---EPSLEDRSWRCNGASFPSQRRKQSYLSR 302
                       +L L    GA    +F+   E  +  R ++ +     S     + LS 
Sbjct: 181 FRRGLYFQPLSWSLRLKIALGAAMGLAFLHSDENKVIYRDFKTSNILLDSD--YNAKLSD 240

Query: 303 FQDIKYTPR-----------------------FSHLSAKSDVYSFGVVLLEILSGRRAID 362
           F   K  P                          HLSAKSDVYSFGVVLLEILSGRRAID
Sbjct: 241 FGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYSFGVVLLEILSGRRAID 300

Query: 363 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 403
           KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP
Sbjct: 301 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 360

BLAST of Cp4.1LG02g13400 vs. NCBI nr
Match: XP_022948607.1 (probable serine/threonine-protein kinase PBL10 [Cucurbita moschata])

HSP 1 Score: 458 bits (1178), Expect = 7.65e-157
Identity = 278/417 (66.67%), Postives = 288/417 (69.06%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSARIKAVSTCSTGT          GGTLNGVSKDAIDISSSSSNSKFSSTSISRS
Sbjct: 1   MGICLSARIKAVSTCSTGT----------GGTLNGVSKDAIDISSSSSNSKFSSTSISRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQCQA-------------------- 242
           AVKRLNLDGLQGHKEWL                 +  C                      
Sbjct: 121 AVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLTRGSLENHL 180

Query: 243 ------------ALSLASTKGAVFSTSFV---EPSLEDRSWRCNGASFPSQRRKQSYLSR 302
                       +L L    GA    +F+   E  +  R ++ +     S     + LS 
Sbjct: 181 FRRGLYFQPLSWSLRLKIALGAAMGLAFLHSDENKVIYRDFKTSNILLDSD--YNAKLSD 240

Query: 303 FQDIKYTPR-----------------------FSHLSAKSDVYSFGVVLLEILSGRRAID 362
           F   K  P                          HLS KSDVYSFGVVLLEILSGRRAID
Sbjct: 241 FGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSGKSDVYSFGVVLLEILSGRRAID 300

Query: 363 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 403
           KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP
Sbjct: 301 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 360

BLAST of Cp4.1LG02g13400 vs. NCBI nr
Match: XP_022998330.1 (probable serine/threonine-protein kinase PBL10 [Cucurbita maxima])

HSP 1 Score: 449 bits (1156), Expect = 1.26e-153
Identity = 274/416 (65.87%), Postives = 285/416 (68.51%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSARIKAVSTCSTGT          GGTLNGVSKDAIDISSSSSNSKFSSTSISRS
Sbjct: 1   MGICLSARIKAVSTCSTGT----------GGTLNGVSKDAIDISSSSSNSKFSSTSISRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEE+SSAAKPGSGTVV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEETSSAAKPGSGTVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQCQA-------------------- 242
           AVKRLNLDGLQGHKEWL                 +  C                      
Sbjct: 121 AVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLTRGSLENHL 180

Query: 243 ------------ALSLASTKGAVFSTSFV---EPSLEDRSWRCNGASFPSQRRKQSYLSR 302
                       +L L    GA    +F+   E  +  R ++ +     S     + LS 
Sbjct: 181 FRRGLYFQPLSWSLRLKIALGAAMGLAFLHSDENKVIYRDFKTSNILLDSD--YNAKLSD 240

Query: 303 FQDIKYTPR-----------------------FSHLSAKSDVYSFGVVLLEILSGRRAID 362
           F   K  P                          HLSAKSDVYSFGVVLLEILSGRRAID
Sbjct: 241 FGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYSFGVVLLEILSGRRAID 300

Query: 363 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 403
           KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP
Sbjct: 301 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 360

BLAST of Cp4.1LG02g13400 vs. ExPASy TrEMBL
Match: A0A6J1G9Q0 (probable serine/threonine-protein kinase PBL10 OS=Cucurbita moschata OX=3662 GN=LOC111452233 PE=3 SV=1)

HSP 1 Score: 458 bits (1178), Expect = 3.70e-157
Identity = 278/417 (66.67%), Postives = 288/417 (69.06%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSARIKAVSTCSTGT          GGTLNGVSKDAIDISSSSSNSKFSSTSISRS
Sbjct: 1   MGICLSARIKAVSTCSTGT----------GGTLNGVSKDAIDISSSSSNSKFSSTSISRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQCQA-------------------- 242
           AVKRLNLDGLQGHKEWL                 +  C                      
Sbjct: 121 AVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLTRGSLENHL 180

Query: 243 ------------ALSLASTKGAVFSTSFV---EPSLEDRSWRCNGASFPSQRRKQSYLSR 302
                       +L L    GA    +F+   E  +  R ++ +     S     + LS 
Sbjct: 181 FRRGLYFQPLSWSLRLKIALGAAMGLAFLHSDENKVIYRDFKTSNILLDSD--YNAKLSD 240

Query: 303 FQDIKYTPR-----------------------FSHLSAKSDVYSFGVVLLEILSGRRAID 362
           F   K  P                          HLS KSDVYSFGVVLLEILSGRRAID
Sbjct: 241 FGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSGKSDVYSFGVVLLEILSGRRAID 300

Query: 363 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 403
           KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP
Sbjct: 301 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 360

BLAST of Cp4.1LG02g13400 vs. ExPASy TrEMBL
Match: A0A6J1KC73 (probable serine/threonine-protein kinase PBL10 OS=Cucurbita maxima OX=3661 GN=LOC111493001 PE=3 SV=1)

HSP 1 Score: 449 bits (1156), Expect = 6.11e-154
Identity = 274/416 (65.87%), Postives = 285/416 (68.51%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSARIKAVSTCSTGT          GGTLNGVSKDAIDISSSSSNSKFSSTSISRS
Sbjct: 1   MGICLSARIKAVSTCSTGT----------GGTLNGVSKDAIDISSSSSNSKFSSTSISRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEE+SSAAKPGSGTVV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEETSSAAKPGSGTVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQCQA-------------------- 242
           AVKRLNLDGLQGHKEWL                 +  C                      
Sbjct: 121 AVKRLNLDGLQGHKEWLAEVNFLGQLHHSHLVRLIGYCLEDEHRLLVYEFLTRGSLENHL 180

Query: 243 ------------ALSLASTKGAVFSTSFV---EPSLEDRSWRCNGASFPSQRRKQSYLSR 302
                       +L L    GA    +F+   E  +  R ++ +     S     + LS 
Sbjct: 181 FRRGLYFQPLSWSLRLKIALGAAMGLAFLHSDENKVIYRDFKTSNILLDSD--YNAKLSD 240

Query: 303 FQDIKYTPR-----------------------FSHLSAKSDVYSFGVVLLEILSGRRAID 362
           F   K  P                          HLSAKSDVYSFGVVLLEILSGRRAID
Sbjct: 241 FGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYSFGVVLLEILSGRRAID 300

Query: 363 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 403
           KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP
Sbjct: 301 KNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRP 360

BLAST of Cp4.1LG02g13400 vs. ExPASy TrEMBL
Match: A0A6J1DQR7 (probable serine/threonine-protein kinase PBL10 OS=Momordica charantia OX=3673 GN=LOC111022260 PE=3 SV=1)

HSP 1 Score: 390 bits (1001), Expect = 2.25e-130
Identity = 240/417 (57.55%), Postives = 263/417 (63.07%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLS+RIKAVSTCSTG            GT NGVS+DA D+SSSSSNSKFSS SI RS
Sbjct: 1   MGICLSSRIKAVSTCSTG------------GTSNGVSRDATDLSSSSSNSKFSSMSIPRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSF+ELKLAT+NFRPDSLLGEGGFGSVYKGWI E S +AAKPGSG VV
Sbjct: 61  EDEILQSSNLKSFSFAELKLATKNFRPDSLLGEGGFGSVYKGWIDEHSLAAAKPGSGMVV 120

Query: 183 AVKRLNLDGLQGHKEWLVS----------------------------------------- 242
           AVKRLNLDG QGHKEWL                                           
Sbjct: 121 AVKRLNLDGFQGHKEWLAEVNFLGQLHHSHLVKLIGYGLEDEHRLLVYEFLTRGSLENHL 180

Query: 243 ------------QCQAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQSYLS 302
                         +  ++L + KG  F  S  E  +  R ++ +     S     + LS
Sbjct: 181 FRRGLYFQPLSWSLRMKIALGAAKGLAFLHSD-ENKVIYRDFKTSNILLDSN--YNAKLS 240

Query: 303 RFQDIKYTPR-----------------------FSHLSAKSDVYSFGVVLLEILSGRRAI 362
            F   K  P                          HLSAKSDVYSFGVVLLE+LSGRRAI
Sbjct: 241 DFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLSAKSDVYSFGVVLLEMLSGRRAI 300

Query: 363 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFR 403
           DKNRPPGEQNLVEWAKPFLANKRKT+RLLD R ERNYSM+SA RVAILASRCLS EPKFR
Sbjct: 301 DKNRPPGEQNLVEWAKPFLANKRKTFRLLDGRIERNYSMDSAFRVAILASRCLSVEPKFR 360

BLAST of Cp4.1LG02g13400 vs. ExPASy TrEMBL
Match: A0A6J1HLY9 (probable serine/threonine-protein kinase PBL10 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464795 PE=3 SV=1)

HSP 1 Score: 387 bits (995), Expect = 1.76e-129
Identity = 243/417 (58.27%), Postives = 264/417 (63.31%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MG+CLSARIKAVSTCSTG            G  NGVS++A D+SSSSSNSKFSS SI RS
Sbjct: 1   MGVCLSARIKAVSTCSTG------------GNSNGVSREANDLSSSSSNSKFSSISIPRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSV+KGWI E S SAAKPGSG VV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVFKGWIDEHSFSAAKPGSGMVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQC---------------------- 242
           AVKRLN+DG QGHKEWL                 +  C                      
Sbjct: 121 AVKRLNVDGHQGHKEWLAEVNFLGQLRHPHLVKLIGYCFEDEHRLLVYEFLPRGSLENHL 180

Query: 243 --------------QAALSLASTKGAVF-------------STSFV------EPSLED-- 302
                         +  ++L + KG  F              TS +         L D  
Sbjct: 181 FKRGLYFQPLSWSLRLKIALGAAKGLAFLHNDENKVIYRDFKTSNILLDTNYNAKLSDFG 240

Query: 303 --RSWRCNGASFPSQRRKQSYLSRFQDIKYTPRFSHLSAKSDVYSFGVVLLEILSGRRAI 362
             +     G S  S R   +Y     +   T    HLSAKSD+YSFGVVLLE+LSGRRAI
Sbjct: 241 LAKDGPTGGKSHVSTRVMGTYGYAAPEYLAT---GHLSAKSDLYSFGVVLLEMLSGRRAI 300

Query: 363 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFR 403
           DKNRPPGEQNLVEWAKPFLANKRKTYRLLD R ERNYSMESA RVAILASRCLSAEPKFR
Sbjct: 301 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDTRIERNYSMESAFRVAILASRCLSAEPKFR 360

BLAST of Cp4.1LG02g13400 vs. ExPASy TrEMBL
Match: A0A6J1I5T6 (probable serine/threonine-protein kinase PBL10 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111469485 PE=3 SV=1)

HSP 1 Score: 386 bits (991), Expect = 7.11e-129
Identity = 241/417 (57.79%), Postives = 268/417 (64.27%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MG+CLSARIKAVSTCSTG            G  NGVS++A D+SSSSSNSKFSS SI RS
Sbjct: 1   MGVCLSARIKAVSTCSTG------------GNSNGVSREANDLSSSSSNSKFSSISIPRS 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSV+KGWI E S SAAKPGSG VV
Sbjct: 61  EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVFKGWIDEHSFSAAKPGSGMVV 120

Query: 183 AVKRLNLDGLQGHKEWL-----------------VSQC---------------------- 242
           AVKRLN+DG QGHKEWL                 +  C                      
Sbjct: 121 AVKRLNVDGHQGHKEWLAEVNFLGQLRHPHLVKLIGYCFEDEHRLLVYEFLPRGSLENHL 180

Query: 243 --------------QAALSLASTKGAVF-------------STSFVEPSLEDRSWRCNGA 302
                         +  ++L + KG  F              TS +   L D ++    +
Sbjct: 181 FKRGLYFQPLSWSLRLKIALGAAKGLAFLHNDENKVIYRDFKTSNI---LLDTNYNAKLS 240

Query: 303 SFPSQRR----KQSYLSRFQDIKY---TPRF---SHLSAKSDVYSFGVVLLEILSGRRAI 362
            F   +      +S++S      Y    P +    HLSAKSD+YSFGVVLLE+LSGRRAI
Sbjct: 241 DFGLAKDGPTGDKSHVSTRVMGTYGYAAPEYLATGHLSAKSDLYSFGVVLLEMLSGRRAI 300

Query: 363 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFR 403
           DKNRPPGEQNLVEWAKPFLANKRKTYRLLD R ERNYSMESA RVAILASRCLSAEPKFR
Sbjct: 301 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDTRIERNYSMESAFRVAILASRCLSAEPKFR 360

BLAST of Cp4.1LG02g13400 vs. TAIR 10
Match: AT2G28930.3 (protein kinase 1B )

HSP 1 Score: 244.2 bits (622), Expect = 1.8e-64
Identity = 176/430 (40.93%), Postives = 227/430 (52.79%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSA+IKAVS    G     +            S +A D S  S +S  S  +  R+
Sbjct: 1   MGICLSAQIKAVSPGKPGASPKYM------------SSEAND-SLGSKSSSVSIRTNPRT 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           E EILQS NLKSF+F+ELK ATRNFRPDS+LGEGGFGSV+KGWI E++ +A+KPG+G V+
Sbjct: 61  EGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVI 120

Query: 183 AVKRLNLDGLQGHKEW-----------------LVSQC---------------------- 242
           AVK+LN DG QGH+EW                 L+  C                      
Sbjct: 121 AVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHL 180

Query: 243 --------------QAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQSYLS 302
                         +  ++L + KG  F  +  E S+  R ++ +     S+   +  LS
Sbjct: 181 FRRGSYFQPLSWTLRLKVALGAAKGLAFLHN-AETSVIYRDFKTSNILLDSEYNAK--LS 240

Query: 303 RFQDIKYTP-----------------------RFSHLSAKSDVYSFGVVLLEILSGRRAI 362
            F   K  P                          HL+ KSDVYS+GVVLLE+LSGRRA+
Sbjct: 241 DFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAV 300

Query: 363 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFR 403
           DKNRPPGEQ LVEWA+P LANKRK +R++D R +  YSME A +VA LA RCL+ E K R
Sbjct: 301 DKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLR 360

BLAST of Cp4.1LG02g13400 vs. TAIR 10
Match: AT2G28930.2 (protein kinase 1B )

HSP 1 Score: 243.8 bits (621), Expect = 2.3e-64
Identity = 176/430 (40.93%), Postives = 228/430 (53.02%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSISRS 122
           MGICLSA+IKAVS                G +   +S +A D S  S +S  S  +  R+
Sbjct: 1   MGICLSAQIKAVS---------------PGASPKYMSSEAND-SLGSKSSSVSIRTNPRT 60

Query: 123 EDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVV 182
           E EILQS NLKSF+F+ELK ATRNFRPDS+LGEGGFGSV+KGWI E++ +A+KPG+G V+
Sbjct: 61  EGEILQSPNLKSFTFAELKAATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVI 120

Query: 183 AVKRLNLDGLQGHKEW-----------------LVSQC---------------------- 242
           AVK+LN DG QGH+EW                 L+  C                      
Sbjct: 121 AVKKLNQDGWQGHQEWLAEVNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHL 180

Query: 243 --------------QAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQSYLS 302
                         +  ++L + KG  F  +  E S+  R ++ +     S+   +  LS
Sbjct: 181 FRRGSYFQPLSWTLRLKVALGAAKGLAFLHN-AETSVIYRDFKTSNILLDSEYNAK--LS 240

Query: 303 RFQDIKYTP-----------------------RFSHLSAKSDVYSFGVVLLEILSGRRAI 362
            F   K  P                          HL+ KSDVYS+GVVLLE+LSGRRA+
Sbjct: 241 DFGLAKDGPTGDKSHVSTRIMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAV 300

Query: 363 DKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFR 403
           DKNRPPGEQ LVEWA+P LANKRK +R++D R +  YSME A +VA LA RCL+ E K R
Sbjct: 301 DKNRPPGEQKLVEWARPLLANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLR 360

BLAST of Cp4.1LG02g13400 vs. TAIR 10
Match: AT1G07570.2 (Protein kinase superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 2.2e-62
Identity = 173/432 (40.05%), Postives = 224/432 (51.85%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSI--- 122
           MGICLSA++KA S+ ++                     DA DI   S  SK SS S+   
Sbjct: 1   MGICLSAQVKAESSGAS------------------TKYDAKDI--GSLGSKASSVSVRPS 60

Query: 123 SRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSG 182
            R+E EILQS NLKSFSF+ELK ATRNFRPDS+LGEGGFG V+KGWI E+S +A++PG+G
Sbjct: 61  PRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTG 120

Query: 183 TVVAVKRLNLDGLQGHKEWLVS-------------------------------------- 242
            V+AVK+LN DG QGH+EWL                                        
Sbjct: 121 LVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLE 180

Query: 243 ---------------QCQAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQS 302
                          + +  ++L + KG  F  S  E  +  R ++ +     S+   + 
Sbjct: 181 NHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHS-SETRVIYRDFKTSNILLDSEYNAK- 240

Query: 303 YLSRFQDIKYTP-----------------------RFSHLSAKSDVYSFGVVLLEILSGR 362
            LS F   K  P                          HL+ KSDVYSFGVVLLE+LSGR
Sbjct: 241 -LSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGR 300

Query: 363 RAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEP 403
           RA+DKNRP GE+NLVEWAKP+L NKRK +R++D R +  YSME A +VA L+ RCL+ E 
Sbjct: 301 RAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEI 360

BLAST of Cp4.1LG02g13400 vs. TAIR 10
Match: AT1G07570.1 (Protein kinase superfamily protein )

HSP 1 Score: 237.3 bits (604), Expect = 2.2e-62
Identity = 173/432 (40.05%), Postives = 224/432 (51.85%), Query Frame = 0

Query: 63  MGICLSARIKAVSTCSTGTDMDILHLSYAGGTLNGVSKDAIDISSSSSNSKFSSTSI--- 122
           MGICLSA++KA S+ ++                     DA DI   S  SK SS S+   
Sbjct: 1   MGICLSAQVKAESSGAS------------------TKYDAKDI--GSLGSKASSVSVRPS 60

Query: 123 SRSEDEILQSSNLKSFSFSELKLATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSG 182
            R+E EILQS NLKSFSF+ELK ATRNFRPDS+LGEGGFG V+KGWI E+S +A++PG+G
Sbjct: 61  PRTEGEILQSPNLKSFSFAELKSATRNFRPDSVLGEGGFGCVFKGWIDEKSLTASRPGTG 120

Query: 183 TVVAVKRLNLDGLQGHKEWLVS-------------------------------------- 242
            V+AVK+LN DG QGH+EWL                                        
Sbjct: 121 LVIAVKKLNQDGWQGHQEWLAEVNYLGQFSHRHLVKLIGYCLEDEHRLLVYEFMPRGSLE 180

Query: 243 ---------------QCQAALSLASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQS 302
                          + +  ++L + KG  F  S  E  +  R ++ +     S+   + 
Sbjct: 181 NHLFRRGLYFQPLSWKLRLKVALGAAKGLAFLHS-SETRVIYRDFKTSNILLDSEYNAK- 240

Query: 303 YLSRFQDIKYTP-----------------------RFSHLSAKSDVYSFGVVLLEILSGR 362
            LS F   K  P                          HL+ KSDVYSFGVVLLE+LSGR
Sbjct: 241 -LSDFGLAKDGPIGDKSHVSTRVMGTHGYAAPEYLATGHLTTKSDVYSFGVVLLELLSGR 300

Query: 363 RAIDKNRPPGEQNLVEWAKPFLANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEP 403
           RA+DKNRP GE+NLVEWAKP+L NKRK +R++D R +  YSME A +VA L+ RCL+ E 
Sbjct: 301 RAVDKNRPSGERNLVEWAKPYLVNKRKIFRVIDNRLQDQYSMEEACKVATLSLRCLTTEI 360

BLAST of Cp4.1LG02g13400 vs. TAIR 10
Match: AT2G28930.1 (protein kinase 1B )

HSP 1 Score: 235.3 bits (599), Expect = 8.3e-62
Identity = 168/411 (40.88%), Postives = 219/411 (53.28%), Query Frame = 0

Query: 86  LHLSYAGGTLNGVSKDAIDISSSSS-NSKFSSTSI---SRSEDEILQSSNLKSFSFSELK 145
           L+ +Y  G   G S   +   ++ S  SK SS SI    R+E EILQS NLKSF+F+ELK
Sbjct: 15  LYANYVFGCCIGASPKYMSSEANDSLGSKSSSVSIRTNPRTEGEILQSPNLKSFTFAELK 74

Query: 146 LATRNFRPDSLLGEGGFGSVYKGWIHEESSSAAKPGSGTVVAVKRLNLDGLQGHKEW--- 205
            ATRNFRPDS+LGEGGFGSV+KGWI E++ +A+KPG+G V+AVK+LN DG QGH+EW   
Sbjct: 75  AATRNFRPDSVLGEGGFGSVFKGWIDEQTLTASKPGTGVVIAVKKLNQDGWQGHQEWLAE 134

Query: 206 --------------LVSQC------------------------------------QAALS 265
                         L+  C                                    +  ++
Sbjct: 135 VNYLGQFSHPNLVKLIGYCLEDEHRLLVYEFMPRGSLENHLFRRGSYFQPLSWTLRLKVA 194

Query: 266 LASTKGAVFSTSFVEPSLEDRSWRCNGASFPSQRRKQSYLSRFQDIKYTP---------- 325
           L + KG  F  +  E S+  R ++ +     S+   +  LS F   K  P          
Sbjct: 195 LGAAKGLAFLHN-AETSVIYRDFKTSNILLDSEYNAK--LSDFGLAKDGPTGDKSHVSTR 254

Query: 326 -------------RFSHLSAKSDVYSFGVVLLEILSGRRAIDKNRPPGEQNLVEWAKPFL 385
                           HL+ KSDVYS+GVVLLE+LSGRRA+DKNRPPGEQ LVEWA+P L
Sbjct: 255 IMGTYGYAAPEYLATGHLTTKSDVYSYGVVLLEVLSGRRAVDKNRPPGEQKLVEWARPLL 314

Query: 386 ANKRKTYRLLDARTERNYSMESASRVAILASRCLSAEPKFRPSMRDVVTTLKDLQDLTGN 403
           ANKRK +R++D R +  YSME A +VA LA RCL+ E K RP+M +VV+ L+ +Q L   
Sbjct: 315 ANKRKLFRVIDNRLQDQYSMEEACKVATLALRCLTFEIKLRPNMNEVVSHLEHIQTLNEA 374

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P465733.3e-6340.93Probable serine/threonine-protein kinase PBL10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q065483.1e-6140.05Probable serine/threonine-protein kinase PBL9 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q65XV83.3e-5538.21Receptor-like cytoplasmic kinase 176 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
P432939.0e-5338.38Probable serine/threonine-protein kinase PBL11 OS=Arabidopsis thaliana OX=3702 G... [more]
O488147.6e-5236.36Serine/threonine-protein kinase BIK1 OS=Arabidopsis thaliana OX=3702 GN=BIK1 PE=... [more]
Match NameE-valueIdentityDescription
KAG6607247.11.65e-16567.91putative serine/threonine-protein kinase PBL10, partial [Cucurbita argyrosperma ... [more]
XP_023523063.11.79e-16268.03probable serine/threonine-protein kinase PBL10 [Cucurbita pepo subsp. pepo][more]
KAG7036931.12.68e-15766.67putative serine/threonine-protein kinase PBL10 [Cucurbita argyrosperma subsp. ar... [more]
XP_022948607.17.65e-15766.67probable serine/threonine-protein kinase PBL10 [Cucurbita moschata][more]
XP_022998330.11.26e-15365.87probable serine/threonine-protein kinase PBL10 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G9Q03.70e-15766.67probable serine/threonine-protein kinase PBL10 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KC736.11e-15465.87probable serine/threonine-protein kinase PBL10 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1DQR72.25e-13057.55probable serine/threonine-protein kinase PBL10 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1HLY91.76e-12958.27probable serine/threonine-protein kinase PBL10 isoform X2 OS=Cucurbita moschata ... [more]
A0A6J1I5T67.11e-12957.79probable serine/threonine-protein kinase PBL10 isoform X3 OS=Cucurbita maxima OX... [more]
Match NameE-valueIdentityDescription
AT2G28930.31.8e-6440.93protein kinase 1B [more]
AT2G28930.22.3e-6440.93protein kinase 1B [more]
AT1G07570.22.2e-6240.05Protein kinase superfamily protein [more]
AT1G07570.12.2e-6240.05Protein kinase superfamily protein [more]
AT2G28930.18.3e-6240.88protein kinase 1B [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 261..356
e-value: 6.0E-7
score: 29.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 239..372
e-value: 1.2E-21
score: 78.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 117..212
e-value: 3.8E-16
score: 61.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 360..387
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 360..403
NoneNo IPR availablePANTHERPTHR45621OS01G0588500 PROTEIN-RELATEDcoord: 100..200
coord: 261..390
NoneNo IPR availablePANTHERPTHR45621:SF184KINASE 1Bcoord: 100..200
coord: 261..390
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 153..185
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 136..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g13400.1Cp4.1LG02g13400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity