Cp4.1LG02g11580 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g11580
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionmetal tolerance protein B-like
LocationCp4.1LG02: 10553611 .. 10554588 (-)
RNA-Seq ExpressionCp4.1LG02g11580
SyntenyCp4.1LG02g11580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAGGAGGAAGTGCCGATTTTAAGAACAGAGCATTCGCTTGATGTTTTGATTCCAGTAATTGCTAAGAAGAGGAATGTTTCTATTCCGAATCCCCTAGATGTAAACTGTTGTCGTTCTGGCTGTCCGTTTTCGAGATTAGAACATAGTAATCTAGAGTCATTAACACGATCGAAGTCCGCTATGAAGCTCGGTGGATTGATATTGTTCTATGTAATCGCTATCATTGTAGAGATTATTGGTGGCTTGAGAGCCAACAGTCTTTCTGTATTGACTGATGCAGCTCACTTGTTGAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCTGTCTGGGTTTCAGGCTGGGCGGCCACACCACAACACTCATTTGGGTTTAATCGCCTTGAAGTTATCGGTGCCCTTGTGTCTGTGCAGCTCATATGGCTGATTTCTGGGTTCTTAATCTACGAGGCAGCCAATCGGATCCTCGGCCACAAGACAAAGGTTAATGGATTGCTCATGTTCGCCATTGCAGCTTTTGGCTTCCTCCTTAACTTAGTTATGGTCATGTGGCTTGGCCATAGCCATAGCCATAGCCATAGCCATAGCCATAGCCATAGCCATGGCCACTCTCATTCTTGTCATCATGATCACTTGGATCATGACACTGACCATGAGGAGGAGGGACATTCACACAGTTCAAGTTCGAACATAAATCTCCAAGGTGCTTATCTTCATCTCATCTCTGATATGATTCAATCTGTTGGTGTGATGGTTGCTGGAGCTGTTCTATGGCTTAAACCCAACTGGGTCGTGGTGGATTTAATCTGTACTCTTGTGTTTTCTGTTCTTGCTTTAGCTACTACTGTTCCCATGCTTAGACATATAGGTGGTATACTGATGGAAGGAACACCCAAGGAGATAAACATTGAGAGGCTAGAACATGATATCAAGAACATCAAAGGTCAAGAACGAAACGTACTGAACGTATAA

mRNA sequence

ATGGCGGAGGAGGAAGTGCCGATTTTAAGAACAGAGCATTCGCTTGATGTTTTGATTCCAGTAATTGCTAAGAAGAGGAATGTTTCTATTCCGAATCCCCTAGATGTAAACTGTTGTCGTTCTGGCTGTCCGTTTTCGAGATTAGAACATAGTAATCTAGAGTCATTAACACGATCGAAGTCCGCTATGAAGCTCGGTGGATTGATATTGTTCTATGTAATCGCTATCATTGTAGAGATTATTGGTGGCTTGAGAGCCAACAGTCTTTCTGTATTGACTGATGCAGCTCACTTGTTGAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCTGTCTGGGTTTCAGGCTGGGCGGCCACACCACAACACTCATTTGGGTTTAATCGCCTTGAAGTTATCGGTGCCCTTGTGTCTGTGCAGCTCATATGGCTGATTTCTGGGTTCTTAATCTACGAGGCAGCCAATCGGATCCTCGGCCACAAGACAAAGGTTAATGGATTGCTCATGTTCGCCATTGCAGCTTTTGGCTTCCTCCTTAACTTAGTTATGGTCATGTGGCTTGGCCATAGCCATAGCCATAGCCATAGCCATAGCCATAGCCATAGCCATGGCCACTCTCATTCTTGTCATCATGATCACTTGGATCATGACACTGACCATGAGGAGGAGGGACATTCACACAGTTCAAGTTCGAACATAAATCTCCAAGGTGCTTATCTTCATCTCATCTCTGATATGATTCAATCTGTTGGTGTGATGGTTGCTGGAGCTGTTCTATGGCTTAAACCCAACTGGGTCGTGGTGGATTTAATCTGTACTCTTGTGTTTTCTGTTCTTGCTTTAGCTACTACTGTTCCCATGCTTAGACATATAGGTGGTATACTGATGGAAGGAACACCCAAGGAGATAAACATTGAGAGGCTAGAACATGATATCAAGAACATCAAAGGTCAAGAACGAAACGTACTGAACGTATAA

Coding sequence (CDS)

ATGGCGGAGGAGGAAGTGCCGATTTTAAGAACAGAGCATTCGCTTGATGTTTTGATTCCAGTAATTGCTAAGAAGAGGAATGTTTCTATTCCGAATCCCCTAGATGTAAACTGTTGTCGTTCTGGCTGTCCGTTTTCGAGATTAGAACATAGTAATCTAGAGTCATTAACACGATCGAAGTCCGCTATGAAGCTCGGTGGATTGATATTGTTCTATGTAATCGCTATCATTGTAGAGATTATTGGTGGCTTGAGAGCCAACAGTCTTTCTGTATTGACTGATGCAGCTCACTTGTTGAGTGATGTTGCTGGTTTCTCTGTTTCCCTCTTTGCTGTCTGGGTTTCAGGCTGGGCGGCCACACCACAACACTCATTTGGGTTTAATCGCCTTGAAGTTATCGGTGCCCTTGTGTCTGTGCAGCTCATATGGCTGATTTCTGGGTTCTTAATCTACGAGGCAGCCAATCGGATCCTCGGCCACAAGACAAAGGTTAATGGATTGCTCATGTTCGCCATTGCAGCTTTTGGCTTCCTCCTTAACTTAGTTATGGTCATGTGGCTTGGCCATAGCCATAGCCATAGCCATAGCCATAGCCATAGCCATAGCCATGGCCACTCTCATTCTTGTCATCATGATCACTTGGATCATGACACTGACCATGAGGAGGAGGGACATTCACACAGTTCAAGTTCGAACATAAATCTCCAAGGTGCTTATCTTCATCTCATCTCTGATATGATTCAATCTGTTGGTGTGATGGTTGCTGGAGCTGTTCTATGGCTTAAACCCAACTGGGTCGTGGTGGATTTAATCTGTACTCTTGTGTTTTCTGTTCTTGCTTTAGCTACTACTGTTCCCATGCTTAGACATATAGGTGGTATACTGATGGAAGGAACACCCAAGGAGATAAACATTGAGAGGCTAGAACATGATATCAAGAACATCAAAGGTCAAGAACGAAACGTACTGAACGTATAA

Protein sequence

MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIKGQERNVLNV
Homology
BLAST of Cp4.1LG02g11580 vs. ExPASy Swiss-Prot
Match: Q6DBM8 (Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 2.5e-77
Identity = 154/282 (54.61%), Postives = 201/282 (71.28%), Query Frame = 0

Query: 43  CPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDV 102
           C F+R EH   E+  R +S  +L  LI  Y+I + V+I+GG +ANSL+V+TDAAHLLSDV
Sbjct: 36  CAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDV 95

Query: 103 AGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKT 162
           AG  VSL A+ VS W A P++SFGF RLEV+ A +SVQLIWL+SG +I+EA  R+L    
Sbjct: 96  AGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSR 155

Query: 163 KVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEE 222
           +VNG +MF I+AFGF +NLVMV+WLGH+HSH H   H H H H H   H H +   + EE
Sbjct: 156 EVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHHHDHHHHHHNHKHQHQHHHKEVVAEEEE 215

Query: 223 E-------GHSHSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLV 282
           E         S S   NIN+QGAYLH ++DMIQS+GVM+ G ++W+KP WV+VDLICTLV
Sbjct: 216 EEMNPLKGEKSSSKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLV 275

Query: 283 FSVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIKG 318
           FS  ALA T+P+L++I GILME  P++++IE+LE  +K I G
Sbjct: 276 FSAFALAATLPILKNIFGILMERVPRDMDIEKLERGLKRIDG 317

BLAST of Cp4.1LG02g11580 vs. ExPASy Swiss-Prot
Match: Q9LXS1 (Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 1.7e-70
Identity = 145/289 (50.17%), Postives = 200/289 (69.20%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS  + S++E+  R+ S  KL   +L   I I+VE++GG++ANSL++LTDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LLSDVA F++SLF++W SGW A PQ S+GF R+E++GALVS+Q+IWL++G L+YEA  R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 158 LGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSHSCHH-----D 217
                +V G LMFA++A G L+N+ M + LGH H H H HSH + HGHSH   H     +
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 218 HLDHDTDHEEEGHS-----HSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVV 277
           H  HD+ H+E   S          N+N+QGAYLH++ D IQSVGVM+ GA++W KP W +
Sbjct: 227 H-HHDSGHDESQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKI 286

Query: 278 VDLICTLVFSVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIK 317
           +DLICTLVFSV+ L TT+ MLR+I  +LME TP+EI+   LE  +  I+
Sbjct: 287 LDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIE 334

BLAST of Cp4.1LG02g11580 vs. ExPASy Swiss-Prot
Match: Q9ZT63 (Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2)

HSP 1 Score: 265.0 bits (676), Expect = 1.1e-69
Identity = 143/303 (47.19%), Postives = 204/303 (67.33%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS  ++++ ++  RS S  KL   ++  ++ + VE++GG++ANSL++LTDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LLSDVA F++SLF++W +GW ATP+ ++GF R+E++GALVS+QLIWL++G L+YEA  RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 158 LGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHS---HGHSH----SCH 217
           +   ++VNG LMF +AAFG ++N++M + LGH H HSH H H H    H HSH    + H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 218 HDHLDHDTDH-----EEEGHSHSS-------------------SSNINLQGAYLHLISDM 277
           H H DH+  H     E++ H+H                       NINLQGAYLH++ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 278 IQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTPKEINIE 310
           IQSVGVM+ GA++W  P W +VDLICTL FSV+ L TT+ M+R+I  +LME TP+EI+  
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

BLAST of Cp4.1LG02g11580 vs. ExPASy Swiss-Prot
Match: Q688R1 (Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 8.0e-68
Identity = 140/330 (42.42%), Postives = 202/330 (61.21%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS   +S+ ++  R  S  KL   ++  +I + VE++GG++ANSL++LTDAAH
Sbjct: 31  CRGAACDFSDSSNSSKDARERMASMRKLIIAVILCIIFMAVEVVGGIKANSLAILTDAAH 90

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LLSDVA F++SLF++W +GW ATPQ S+GF R+E++GALVS+QLIWL++G L+YEA  R+
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPQQSYGFFRIEILGALVSIQLIWLLAGILVYEAIVRL 150

Query: 158 LGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSH---SCHHDHL 217
           +    +V G LMFA++AFG  +N++M + LGH H H H H H H H H H      HDH 
Sbjct: 151 INESGEVQGSLMFAVSAFGLFVNIIMAVLLGHDHGHGHGHGHGHGHSHDHDHGGSDHDHH 210

Query: 218 DHD------TDHEEEGHSHS---------------------------------------- 277
            H+        H E+GH +S                                        
Sbjct: 211 HHEDQEHGHVHHHEDGHGNSITVNLHHHPGTGHHHHDAEEPLLKSDAGCDSTQSGAKDAK 270

Query: 278 -SSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPM 318
            +  NIN+  AYLH++ D IQS+GVM+ GA++W KP W ++DLICTL+FSV+ L TT+ M
Sbjct: 271 KARRNINVHSAYLHVLGDSIQSIGVMIGGAIIWYKPEWKIIDLICTLIFSVIVLFTTIKM 330

BLAST of Cp4.1LG02g11580 vs. ExPASy Swiss-Prot
Match: Q9M271 (Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 3.6e-60
Identity = 122/280 (43.57%), Postives = 185/280 (66.07%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS    S+ ++  R+ S  KL  +++  ++ + +E++ G++ANSL++L DAAH
Sbjct: 9   CGETACGFST---SSSDAKKRAASMRKLCFVVVLCLLFMSIEVVCGIKANSLAILADAAH 68

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LL+DV  F++S+ ++W S W A P+ S+GF R+E++G LVS+QLIWL++G L+YEA  R+
Sbjct: 69  LLTDVGAFAISMLSLWASSWEANPRQSYGFFRIEILGTLVSIQLIWLLTGILVYEAVTRL 128

Query: 158 LGH-KTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDH 217
           +      V+G  M  +AAFG ++N++M++ LGH H H H H HSH HGHS+    + L  
Sbjct: 129 VQETNDDVDGFFMVLVAAFGLVVNIIMIVVLGHDHGHGHDHGHSHDHGHSYGERAEQLLE 188

Query: 218 DTDHEEEGHSHSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVF 277
            +             NIN+QGAYLH++ D+IQS+GVM+ G ++W  P W V+DLICTL F
Sbjct: 189 KS---------KEIRNINVQGAYLHVLGDLIQSIGVMIGGGMIWYNPKWKVIDLICTLFF 248

Query: 278 SVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIK 317
           SV+ L TT+ MLR I  +LME TP+EI+  +LE  +  I+
Sbjct: 249 SVIVLGTTIKMLRSILEVLMESTPREIDARQLEKGLMEIE 276

BLAST of Cp4.1LG02g11580 vs. NCBI nr
Match: XP_023523132.1 (metal tolerance protein B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 610 bits (1574), Expect = 6.84e-219
Identity = 317/317 (100.00%), Postives = 317/317 (100.00%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK
Sbjct: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT
Sbjct: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN
Sbjct: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240
           LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL
Sbjct: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240

Query: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300
           HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP
Sbjct: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300

Query: 301 KEINIERLEHDIKNIKG 317
           KEINIERLEHDIKNIKG
Sbjct: 301 KEINIERLEHDIKNIKG 317

BLAST of Cp4.1LG02g11580 vs. NCBI nr
Match: KAG6607273.1 (Metal tolerance protein B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 608 bits (1569), Expect = 3.95e-218
Identity = 316/317 (99.68%), Postives = 316/317 (99.68%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK
Sbjct: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT
Sbjct: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN
Sbjct: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240
           LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHD DHEEEGHSHSSSSNINLQGAYL
Sbjct: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDADHEEEGHSHSSSSNINLQGAYL 240

Query: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300
           HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP
Sbjct: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300

Query: 301 KEINIERLEHDIKNIKG 317
           KEINIERLEHDIKNIKG
Sbjct: 301 KEINIERLEHDIKNIKG 317

BLAST of Cp4.1LG02g11580 vs. NCBI nr
Match: KAG7036951.1 (Metal tolerance protein B, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 608 bits (1569), Expect = 3.95e-218
Identity = 316/317 (99.68%), Postives = 316/317 (99.68%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK
Sbjct: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT
Sbjct: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN
Sbjct: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240
           LVMVMWLGHSHSHSHSH HSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL
Sbjct: 181 LVMVMWLGHSHSHSHSHRHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240

Query: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300
           HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP
Sbjct: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300

Query: 301 KEINIERLEHDIKNIKG 317
           KEINIERLEHDIKNIKG
Sbjct: 301 KEINIERLEHDIKNIKG 317

BLAST of Cp4.1LG02g11580 vs. NCBI nr
Match: XP_022998906.1 (metal tolerance protein B-like [Cucurbita maxima])

HSP 1 Score: 583 bits (1503), Expect = 3.33e-208
Identity = 306/317 (96.53%), Postives = 307/317 (96.85%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEVPILRTEHSLDVLIPV+AKKRNVSIPNP DVNCCRSGCPFS LEHSNLESLTRSK
Sbjct: 1   MAEEEVPILRTEHSLDVLIPVLAKKRNVSIPNPPDVNCCRSGCPFSGLEHSNLESLTRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT
Sbjct: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN
Sbjct: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240
           LVMVMWLGHSHSHSH        GHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL
Sbjct: 181 LVMVMWLGHSHSHSH--------GHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240

Query: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300
           HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP
Sbjct: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300

Query: 301 KEINIERLEHDIKNIKG 317
           KEINIERLEHDIKNIKG
Sbjct: 301 KEINIERLEHDIKNIKG 309

BLAST of Cp4.1LG02g11580 vs. NCBI nr
Match: XP_022948628.1 (metal tolerance protein B-like [Cucurbita moschata])

HSP 1 Score: 580 bits (1496), Expect = 3.61e-207
Identity = 305/317 (96.21%), Postives = 305/317 (96.21%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK
Sbjct: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT
Sbjct: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILG KTKVNGLLMFAIAAFGFLLN
Sbjct: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGQKTKVNGLLMFAIAAFGFLLN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240
           LVMVMWLGHSHSHSHSHS          CHHDHLDHDTDHEEEGHSHSSS NINLQGAYL
Sbjct: 181 LVMVMWLGHSHSHSHSHS----------CHHDHLDHDTDHEEEGHSHSSSPNINLQGAYL 240

Query: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300
           HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP
Sbjct: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300

Query: 301 KEINIERLEHDIKNIKG 317
           KEINIERLEHDIKNIKG
Sbjct: 301 KEINIERLEHDIKNIKG 307

BLAST of Cp4.1LG02g11580 vs. ExPASy TrEMBL
Match: A0A6J1KBI4 (metal tolerance protein B-like OS=Cucurbita maxima OX=3661 GN=LOC111493429 PE=4 SV=1)

HSP 1 Score: 583 bits (1503), Expect = 1.61e-208
Identity = 306/317 (96.53%), Postives = 307/317 (96.85%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEVPILRTEHSLDVLIPV+AKKRNVSIPNP DVNCCRSGCPFS LEHSNLESLTRSK
Sbjct: 1   MAEEEVPILRTEHSLDVLIPVLAKKRNVSIPNPPDVNCCRSGCPFSGLEHSNLESLTRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT
Sbjct: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN
Sbjct: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240
           LVMVMWLGHSHSHSH        GHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL
Sbjct: 181 LVMVMWLGHSHSHSH--------GHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240

Query: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300
           HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP
Sbjct: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300

Query: 301 KEINIERLEHDIKNIKG 317
           KEINIERLEHDIKNIKG
Sbjct: 301 KEINIERLEHDIKNIKG 309

BLAST of Cp4.1LG02g11580 vs. ExPASy TrEMBL
Match: A0A6J1G9U1 (metal tolerance protein B-like OS=Cucurbita moschata OX=3662 GN=LOC111452250 PE=4 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 1.75e-207
Identity = 305/317 (96.21%), Postives = 305/317 (96.21%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK
Sbjct: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT
Sbjct: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILG KTKVNGLLMFAIAAFGFLLN
Sbjct: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGQKTKVNGLLMFAIAAFGFLLN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEEEGHSHSSSSNINLQGAYL 240
           LVMVMWLGHSHSHSHSHS          CHHDHLDHDTDHEEEGHSHSSS NINLQGAYL
Sbjct: 181 LVMVMWLGHSHSHSHSHS----------CHHDHLDHDTDHEEEGHSHSSSPNINLQGAYL 240

Query: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300
           HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP
Sbjct: 241 HLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTP 300

Query: 301 KEINIERLEHDIKNIKG 317
           KEINIERLEHDIKNIKG
Sbjct: 301 KEINIERLEHDIKNIKG 307

BLAST of Cp4.1LG02g11580 vs. ExPASy TrEMBL
Match: A0A1S3C537 (metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1)

HSP 1 Score: 452 bits (1163), Expect = 2.15e-156
Identity = 244/341 (71.55%), Postives = 274/341 (80.35%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEV IL+TEH  +V +P++AKKRNV IP   +V CC SGC FSRLEHSNLESL RSK
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGG+ILFY IAI+VEIIGGLRANSLSV+TDAAHLLSDVAGFSVSLFAVWVSGW AT
Sbjct: 61  SAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFG+NRLEV+GALVSVQLIWLISG LIYEA +RIL  KTKV+G LMFA+AAFGF+LN
Sbjct: 121 PQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDH----------LDHDTDHEEE------- 240
           L MV+WLGHSH H HSHS       SH CHHDH          L H++DHE+E       
Sbjct: 181 LFMVIWLGHSHHHHHSHS-------SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTK 240

Query: 241 -------GHSHSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVF 300
                     +SS+ NINLQGAYLH+I+DMIQS+GVM+AG VLW KP W+VVDLICTLVF
Sbjct: 241 LEGASLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVF 300

Query: 301 SVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIKG 317
           SVLALATT  MLRHI  ILMEGTP+E++IE LE+DIKNIKG
Sbjct: 301 SVLALATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKG 334

BLAST of Cp4.1LG02g11580 vs. ExPASy TrEMBL
Match: A0A5D3BGQ9 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G001790 PE=4 SV=1)

HSP 1 Score: 452 bits (1163), Expect = 5.88e-156
Identity = 244/341 (71.55%), Postives = 274/341 (80.35%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEV IL+TEH  +V +P++AKKRNV IP   +V CC SGC FSRLEHSNLESL RSK
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGG+ILFY IAI+VEIIGGLRANSLSV+TDAAHLLSDVAGFSVSLFAVWVSGW AT
Sbjct: 61  SAMKLGGMILFYTIAIVVEIIGGLRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEAT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFG+NRLEV+GALVSVQLIWLISG LIYEA +RIL  KTKV+G LMFA+AAFGF+LN
Sbjct: 121 PQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDH----------LDHDTDHEEE------- 240
           L MV+WLGHSH H HSHS       SH CHHDH          L H++DHE+E       
Sbjct: 181 LFMVIWLGHSHHHHHSHS-------SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTK 240

Query: 241 -------GHSHSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVF 300
                     +SS+ NINLQGAYLH+I+DMIQS+GVM+AG VLW KP W+VVDLICTLVF
Sbjct: 241 LEGASLGSKDNSSTLNINLQGAYLHVITDMIQSIGVMIAGFVLWFKPEWIVVDLICTLVF 300

Query: 301 SVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIKG 317
           SVLALATT  MLRHI  ILMEGTP+E++IE LE+DIKNIKG
Sbjct: 301 SVLALATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKG 334

BLAST of Cp4.1LG02g11580 vs. ExPASy TrEMBL
Match: A0A5A7SNE8 (Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold848G00010 PE=4 SV=1)

HSP 1 Score: 448 bits (1152), Expect = 1.01e-154
Identity = 241/341 (70.67%), Postives = 272/341 (79.77%), Query Frame = 0

Query: 1   MAEEEVPILRTEHSLDVLIPVIAKKRNVSIPNPLDVNCCRSGCPFSRLEHSNLESLTRSK 60
           MAEEEV IL+TEH  +V +P++AKKRNV IP   +V CC SGC FSRLEHSNLESL RSK
Sbjct: 1   MAEEEVLILKTEHWDEVKMPIVAKKRNVVIPTSSEVKCCNSGCAFSRLEHSNLESLKRSK 60

Query: 61  SAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDVAGFSVSLFAVWVSGWAAT 120
           SAMKLGG+ILFY IAI+VEIIGG RANSLSV+TDAAHLLSDVAGFSVSLFAVWVSGW  T
Sbjct: 61  SAMKLGGMILFYTIAIVVEIIGGFRANSLSVMTDAAHLLSDVAGFSVSLFAVWVSGWEVT 120

Query: 121 PQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKTKVNGLLMFAIAAFGFLLN 180
           PQHSFG+NRLEV+GALVSVQLIWLISG LIYEA +RIL  KTKV+G LMFA+AAFGF+LN
Sbjct: 121 PQHSFGYNRLEVLGALVSVQLIWLISGVLIYEAIDRILAQKTKVDGFLMFAVAAFGFILN 180

Query: 181 LVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDH----------LDHDTDHEEE------- 240
           L MV+WLGHSH H HSHS       SH CHHDH          L H++DHE+E       
Sbjct: 181 LFMVIWLGHSHHHHHSHS-------SHCCHHDHHSHSHSDQNHLGHESDHEKEEIYTLTK 240

Query: 241 -------GHSHSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLVF 300
                     +SS+ NINLQGAYLH+I+D+IQS+GVM+AG VLW KP W+VVDLICTLVF
Sbjct: 241 LEGASLGSKDNSSTLNINLQGAYLHVITDLIQSIGVMIAGFVLWFKPEWIVVDLICTLVF 300

Query: 301 SVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIKG 317
           SVLALATT  MLRHI  ILMEGTP+E++IE LE+DIKNIKG
Sbjct: 301 SVLALATTFSMLRHIAVILMEGTPREVHIESLENDIKNIKG 334

BLAST of Cp4.1LG02g11580 vs. TAIR 10
Match: AT2G29410.1 (metal tolerance protein B1 )

HSP 1 Score: 290.4 bits (742), Expect = 1.8e-78
Identity = 154/282 (54.61%), Postives = 201/282 (71.28%), Query Frame = 0

Query: 43  CPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAHLLSDV 102
           C F+R EH   E+  R +S  +L  LI  Y+I + V+I+GG +ANSL+V+TDAAHLLSDV
Sbjct: 36  CAFTRQEHCVSETKEREESTRRLSSLIFLYLIVMSVQIVGGFKANSLAVMTDAAHLLSDV 95

Query: 103 AGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRILGHKT 162
           AG  VSL A+ VS W A P++SFGF RLEV+ A +SVQLIWL+SG +I+EA  R+L    
Sbjct: 96  AGLCVSLLAIKVSSWEANPRNSFGFKRLEVLAAFLSVQLIWLVSGVIIHEAIQRLLSRSR 155

Query: 163 KVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSHSCHHDHLDHDTDHEE 222
           +VNG +MF I+AFGF +NLVMV+WLGH+HSH H   H H H H H   H H +   + EE
Sbjct: 156 EVNGEIMFGISAFGFFMNLVMVLWLGHNHSHHHHDHHHHHHNHKHQHQHHHKEVVAEEEE 215

Query: 223 E-------GHSHSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVVVDLICTLV 282
           E         S S   NIN+QGAYLH ++DMIQS+GVM+ G ++W+KP WV+VDLICTLV
Sbjct: 216 EEMNPLKGEKSSSKEMNINIQGAYLHAMADMIQSLGVMIGGGIIWVKPKWVLVDLICTLV 275

Query: 283 FSVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIKG 318
           FS  ALA T+P+L++I GILME  P++++IE+LE  +K I G
Sbjct: 276 FSAFALAATLPILKNIFGILMERVPRDMDIEKLERGLKRIDG 317

BLAST of Cp4.1LG02g11580 vs. TAIR 10
Match: AT3G58810.1 (metal tolerance protein A2 )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 145/289 (50.17%), Postives = 200/289 (69.20%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS  + S++E+  R+ S  KL   +L   I I+VE++GG++ANSL++LTDAAH
Sbjct: 86  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 145

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LLSDVA F++SLF++W SGW A PQ S+GF R+E++GALVS+Q+IWL++G L+YEA  R+
Sbjct: 146 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 205

Query: 158 LGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSHSCHH-----D 217
                +V G LMFA++A G L+N+ M + LGH H H H HSH + HGHSH   H     +
Sbjct: 206 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 265

Query: 218 HLDHDTDHEEEGHS-----HSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVV 277
           H  HD+ H+E   S          N+N+QGAYLH++ D IQSVGVM+ GA++W KP W +
Sbjct: 266 H-HHDSGHDESQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKI 325

Query: 278 VDLICTLVFSVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIK 317
           +DLICTLVFSV+ L TT+ MLR+I  +LME TP+EI+   LE  +  I+
Sbjct: 326 LDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIE 373

BLAST of Cp4.1LG02g11580 vs. TAIR 10
Match: AT3G58810.2 (metal tolerance protein A2 )

HSP 1 Score: 267.7 bits (683), Expect = 1.2e-71
Identity = 145/289 (50.17%), Postives = 200/289 (69.20%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS  + S++E+  R+ S  KL   +L   I I+VE++GG++ANSL++LTDAAH
Sbjct: 47  CGEAPCGFSDAKTSSIEAQERAASMRKLLIAVLLCAIFIVVEVVGGIKANSLAILTDAAH 106

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LLSDVA F++SLF++W SGW A PQ S+GF R+E++GALVS+Q+IWL++G L+YEA  R+
Sbjct: 107 LLSDVAAFAISLFSLWASGWKANPQQSYGFFRIEILGALVSIQMIWLLAGILVYEAIVRL 166

Query: 158 LGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHSHGHSHSCHH-----D 217
                +V G LMFA++A G L+N+ M + LGH H H H HSH + HGHSH   H     +
Sbjct: 167 NNGSGEVEGSLMFAVSAVGLLVNIAMAILLGHDHGHGHGHSHDNGHGHSHDHGHGIAATE 226

Query: 218 HLDHDTDHEEEGHS-----HSSSSNINLQGAYLHLISDMIQSVGVMVAGAVLWLKPNWVV 277
           H  HD+ H+E   S          N+N+QGAYLH++ D IQSVGVM+ GA++W KP W +
Sbjct: 227 H-HHDSGHDESQLSDVLIEQKKQRNVNIQGAYLHVLGDSIQSVGVMIGGAIIWYKPEWKI 286

Query: 278 VDLICTLVFSVLALATTVPMLRHIGGILMEGTPKEINIERLEHDIKNIK 317
           +DLICTLVFSV+ L TT+ MLR+I  +LME TP+EI+   LE  +  I+
Sbjct: 287 LDLICTLVFSVIVLGTTIGMLRNILEVLMESTPREIDPTMLEKGVCEIE 334

BLAST of Cp4.1LG02g11580 vs. TAIR 10
Match: AT2G46800.1 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 265.0 bits (676), Expect = 7.9e-71
Identity = 143/303 (47.19%), Postives = 204/303 (67.33%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS  ++++ ++  RS S  KL   ++  ++ + VE++GG++ANSL++LTDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LLSDVA F++SLF++W +GW ATP+ ++GF R+E++GALVS+QLIWL++G L+YEA  RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 158 LGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHS---HGHSH----SCH 217
           +   ++VNG LMF +AAFG ++N++M + LGH H HSH H H H    H HSH    + H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 218 HDHLDHDTDH-----EEEGHSHSS-------------------SSNINLQGAYLHLISDM 277
           H H DH+  H     E++ H+H                       NINLQGAYLH++ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 278 IQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTPKEINIE 310
           IQSVGVM+ GA++W  P W +VDLICTL FSV+ L TT+ M+R+I  +LME TP+EI+  
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

BLAST of Cp4.1LG02g11580 vs. TAIR 10
Match: AT2G46800.2 (zinc transporter of Arabidopsis thaliana )

HSP 1 Score: 265.0 bits (676), Expect = 7.9e-71
Identity = 143/303 (47.19%), Postives = 204/303 (67.33%), Query Frame = 0

Query: 38  CCRSGCPFSRLEHSNLESLTRSKSAMKLGGLILFYVIAIIVEIIGGLRANSLSVLTDAAH 97
           C  + C FS  ++++ ++  RS S  KL   ++  ++ + VE++GG++ANSL++LTDAAH
Sbjct: 31  CGEAPCGFSDSKNASGDAHERSASMRKLCIAVVLCLVFMSVEVVGGIKANSLAILTDAAH 90

Query: 98  LLSDVAGFSVSLFAVWVSGWAATPQHSFGFNRLEVIGALVSVQLIWLISGFLIYEAANRI 157
           LLSDVA F++SLF++W +GW ATP+ ++GF R+E++GALVS+QLIWL++G L+YEA  RI
Sbjct: 91  LLSDVAAFAISLFSLWAAGWEATPRQTYGFFRIEILGALVSIQLIWLLTGILVYEAIIRI 150

Query: 158 LGHKTKVNGLLMFAIAAFGFLLNLVMVMWLGHSHSHSHSHSHSHS---HGHSH----SCH 217
           +   ++VNG LMF +AAFG ++N++M + LGH H HSH H H H    H HSH    + H
Sbjct: 151 VTETSEVNGFLMFLVAAFGLVVNIIMAVLLGHDHGHSHGHGHGHGHDHHNHSHGVTVTTH 210

Query: 218 HDHLDHDTDH-----EEEGHSHSS-------------------SSNINLQGAYLHLISDM 277
           H H DH+  H     E++ H+H                       NINLQGAYLH++ D 
Sbjct: 211 HHHHDHEHGHSHGHGEDKHHAHGDVTEQLLDKSKTQVAAKEKRKRNINLQGAYLHVLGDS 270

Query: 278 IQSVGVMVAGAVLWLKPNWVVVDLICTLVFSVLALATTVPMLRHIGGILMEGTPKEINIE 310
           IQSVGVM+ GA++W  P W +VDLICTL FSV+ L TT+ M+R+I  +LME TP+EI+  
Sbjct: 271 IQSVGVMIGGAIIWYNPEWKIVDLICTLAFSVIVLGTTINMIRNILEVLMESTPREIDAT 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6DBM82.5e-7754.61Metal tolerance protein B OS=Arabidopsis thaliana OX=3702 GN=MTPB PE=2 SV=1[more]
Q9LXS11.7e-7050.17Metal tolerance protein A2 OS=Arabidopsis thaliana OX=3702 GN=MTPA2 PE=2 SV=2[more]
Q9ZT631.1e-6947.19Metal tolerance protein 1 OS=Arabidopsis thaliana OX=3702 GN=MTP1 PE=1 SV=2[more]
Q688R18.0e-6842.42Metal tolerance protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MTP1 PE=2 ... [more]
Q9M2713.6e-6043.57Metal tolerance protein A1 OS=Arabidopsis thaliana OX=3702 GN=MTPA1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023523132.16.84e-219100.00metal tolerance protein B-like [Cucurbita pepo subsp. pepo][more]
KAG6607273.13.95e-21899.68Metal tolerance protein B, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7036951.13.95e-21899.68Metal tolerance protein B, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022998906.13.33e-20896.53metal tolerance protein B-like [Cucurbita maxima][more]
XP_022948628.13.61e-20796.21metal tolerance protein B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1KBI41.61e-20896.53metal tolerance protein B-like OS=Cucurbita maxima OX=3661 GN=LOC111493429 PE=4 ... [more]
A0A6J1G9U11.75e-20796.21metal tolerance protein B-like OS=Cucurbita moschata OX=3662 GN=LOC111452250 PE=... [more]
A0A1S3C5372.15e-15671.55metal tolerance protein B OS=Cucumis melo OX=3656 GN=LOC103497126 PE=4 SV=1[more]
A0A5D3BGQ95.88e-15671.55Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A5A7SNE81.01e-15470.67Metal tolerance protein B OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo... [more]
Match NameE-valueIdentityDescription
AT2G29410.11.8e-7854.61metal tolerance protein B1 [more]
AT3G58810.11.2e-7150.17metal tolerance protein A2 [more]
AT3G58810.21.2e-7150.17metal tolerance protein A2 [more]
AT2G46800.17.9e-7147.19zinc transporter of Arabidopsis thaliana [more]
AT2G46800.27.9e-7147.19zinc transporter of Arabidopsis thaliana [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002524Cation efflux proteinTIGRFAMTIGR01297TIGR01297coord: 69..317
e-value: 6.4E-61
score: 204.1
IPR002524Cation efflux proteinPFAMPF01545Cation_effluxcoord: 69..296
e-value: 5.7E-38
score: 130.7
IPR027469Cation efflux transmembrane domain superfamilyGENE3D1.20.1510.10Cation efflux protein transmembrane domaincoord: 65..295
e-value: 8.8E-44
score: 151.5
IPR027469Cation efflux transmembrane domain superfamilySUPERFAMILY161111Cation efflux protein transmembrane domain-likecoord: 67..297
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 208..227
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 197..231
NoneNo IPR availablePANTHERPTHR11562CATION EFFLUX PROTEIN/ ZINC TRANSPORTERcoord: 4..318
NoneNo IPR availablePANTHERPTHR11562:SF54METAL TOLERANCE PROTEIN Bcoord: 4..318

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g11580.1Cp4.1LG02g11580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071577 zinc ion transmembrane transport
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0005385 zinc ion transmembrane transporter activity
molecular_function GO:0008324 cation transmembrane transporter activity