Cp4.1LG02g11400 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g11400
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprotein NETWORKED 4A-like
LocationCp4.1LG02: 10207730 .. 10209298 (+)
RNA-Seq ExpressionCp4.1LG02g11400
SyntenyCp4.1LG02g11400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGACATGGACCGCAGCATCAAACATATGTTGAGGCTAATAGAAGAGGATGCAGATTCATTTGCCAAGAAGGCTGAGATGTATTATCAGAAGAGGCCAATGATGATCTCTTACATTTGGGAGTTCTACACCACATACCGTTCCCTTGTTGAGCGTTATGATCATGTAACAGGAGAGCTCCGAAGAAATCNATTTGCCAAGAAGGCTGAGATGTATTATCAGAAGAGGCCAGTGATGATCTCTTACATTTGGGAGTTCTACACCACATACCGTTCCCTTGTTGAGCGTTATGATCATGTCACAGGAGAGCTCCGAAGAAATATCCCTTCAGATCTACAATCCCACGGCTCTGATATCTGTGATCTTATCTCTGAGCCACGAGCTGCTGGCTTTGATGTCTTCCTTGGATCTGGTGGCAGCAACTCTGATACTTGCCAAAAGGAAGGATATGATTCATCTTTTTTCACAGATTCTGAACCTGGATCTGATGATTCCTCTGTGTACAATTATTCAGATGGTGAANAGGAAGGATATGATTCATCTTTTTTCACAGATTCTGAACCTGGTTCTGATGATTCCTCGGTGTACTATTCAGATGGTGATCAAGGACTGAACAGGAAGATGATGGAATTGGAGAATGAGCTGAGAGAGGCTAAAGAACAGCTCCACATGAAGGAGGATAATGCAGAAGAATGTTCATTTCCATTCAAAGCGGCAACAGATGAAAATTCAGACATTCAAGTTATGAGGTTGAAAAGTGAGCACCAGAAGAATGTGCTATCAGAATTGACCAAGGATTTGCAGGCTGAGTCAGTGTCCGCTACAATGGCAGATACTCAGAAGCATGAGGATGGGATTCTAGTGGAAGAGCTAAGAATTACAAAGGAAAAACTTGAGAACTCGCAGAAAGAACATTCTAAATTAAAATTTGAACTTGAGAACAATAAAGCACCAGAGAAAATCTGCCATTTGCCGGATGAGCTTGAAGCTGCTCGAAAAGATACTGACACATGGAAGGACAAGGTGAGTGCAGATAGAAGAGCGGTCGCCGAGCTCCAAGAAAGAATTTCAAGGTTGAAAGCTAGAGATCTTAAACTTGCAGTATCTAATGCTGAGCATAAAATTTTTCCTGAGAATGTAACGATTACGGCTGAAATATCTCAGCCACCTAAGGAACAAGCTCTGTTGGAGGATGAGATCAGAAAAGTCAAGACAGAAAAGGAGGATCTGAAGGAGAGACTTAACCGCGAGATTGAGAATCTTATAAGTGGCCACAACGGCTTCCAATCTGAAAGAGATCAGCTCCACGCAGAACTTATTACTTTAAAAGAGAATTTGAGCTCTAAAGGCAAGCTTGTGCAAAAATTGGAAAGGGAACGTGTGGAACTAGAAGGGAAGGTGGAGAGGCAAAGAGTAGTGATAATGGAAGGAGCAGAGGAAAAAAGAGAGGCTATAAGGCAACTATGTTTTTCAATTGAGCATTACAGGAGTGGATATCATATGCTTAGAGAAGTTTTTATCAGTCAAAAGCGGGATCTCGGGTTCGCATCTCAAGGCAAGTCATGA

mRNA sequence

ATGTTAGACATGGACCGCAGCATCAAACATATGTTGAGGCTAATAGAAGAGGATGCAGATTCATTTGCCAAGAAGGCTGAGATGTATTATCAGAAGAGGCCAATGATGATCTCTTACATTTGGGAGTTCTACACCACATACCGTTCCCTTGTTGAGCGTTATGATCATGTAACAGGAGAGCTCCGAAGAAATCNATTTGCCAAGAAGGCTGAGATGTATTATCAGAAGAGGCCAGTGATGATCTCTTACATTTGGGAGTTCTACACCACATACCGTTCCCTTGTTGAGCGTTATGATCATGTCACAGGAGAGCTCCGAAGAAATATCCCTTCAGATCTACAATCCCACGGCTCTGATATCTGTGATCTTATCTCTGAGCCACGAGCTGCTGGCTTTGATGTCTTCCTTGGATCTGGTGGCAGCAACTCTGATACTTGCCAAAAGGAAGGATATGATTCATCTTTTTTCACAGATTCTGAACCTGGATCTGATGATTCCTCTGTGTACAATTATTCAGATGGTGAANAGGAAGGATATGATTCATCTTTTTTCACAGATTCTGAACCTGGTTCTGATGATTCCTCGGTGTACTATTCAGATGGTGATCAAGGACTGAACAGGAAGATGATGGAATTGGAGAATGAGCTGAGAGAGGCTAAAGAACAGCTCCACATGAAGGAGGATAATGCAGAAGAATGTTCATTTCCATTCAAAGCGGCAACAGATGAAAATTCAGACATTCAAGTTATGAGGTTGAAAAGTGAGCACCAGAAGAATGTGCTATCAGAATTGACCAAGGATTTGCAGGCTGAGTCAGTGTCCGCTACAATGGCAGATACTCAGAAGCATGAGGATGGGATTCTAGTGGAAGAGCTAAGAATTACAAAGGAAAAACTTGAGAACTCGCAGAAAGAACATTCTAAATTAAAATTTGAACTTGAGAACAATAAAGCACCAGAGAAAATCTGCCATTTGCCGGATGAGCTTGAAGCTGCTCGAAAAGATACTGACACATGGAAGGACAAGGTGAGTGCAGATAGAAGAGCGGTCGCCGAGCTCCAAGAAAGAATTTCAAGGTTGAAAGCTAGAGATCTTAAACTTGCAGTATCTAATGCTGAGCATAAAATTTTTCCTGAGAATGTAACGATTACGGCTGAAATATCTCAGCCACCTAAGGAACAAGCTCTGTTGGAGGATGAGATCAGAAAAGTCAAGACAGAAAAGGAGGATCTGAAGGAGAGACTTAACCGCGAGATTGAGAATCTTATAAGTGGCCACAACGGCTTCCAATCTGAAAGAGATCAGCTCCACGCAGAACTTATTACTTTAAAAGAGAATTTGAGCTCTAAAGGCAAGCTTGTGCAAAAATTGGAAAGGGAACGTGTGGAACTAGAAGGGAAGGTGGAGAGGCAAAGAGTAGTGATAATGGAAGGAGCAGAGGAAAAAAGAGAGGCTATAAGGCAACTATGTTTTTCAATTGAGCATTACAGGAGTGGATATCATATGCTTAGAGAAGTTTTTATCAGTCAAAAGCGGGATCTCGGGTTCGCATCTCAAGGCAAGTCATGA

Coding sequence (CDS)

ATGTTAGACATGGACCGCAGCATCAAACATATGTTGAGGCTAATAGAAGAGGATGCAGATTCATTTGCCAAGAAGGCTGAGATGTATTATCAGAAGAGGCCAATGATGATCTCTTACATTTGGGAGTTCTACACCACATACCGTTCCCTTGTTGAGCGTTATGATCATGTAACAGGAGAGCTCCGAAGAAATCNATTTGCCAAGAAGGCTGAGATGTATTATCAGAAGAGGCCAGTGATGATCTCTTACATTTGGGAGTTCTACACCACATACCGTTCCCTTGTTGAGCGTTATGATCATGTCACAGGAGAGCTCCGAAGAAATATCCCTTCAGATCTACAATCCCACGGCTCTGATATCTGTGATCTTATCTCTGAGCCACGAGCTGCTGGCTTTGATGTCTTCCTTGGATCTGGTGGCAGCAACTCTGATACTTGCCAAAAGGAAGGATATGATTCATCTTTTTTCACAGATTCTGAACCTGGATCTGATGATTCCTCTGTGTACAATTATTCAGATGGTGAANAGGAAGGATATGATTCATCTTTTTTCACAGATTCTGAACCTGGTTCTGATGATTCCTCGGTGTACTATTCAGATGGTGATCAAGGACTGAACAGGAAGATGATGGAATTGGAGAATGAGCTGAGAGAGGCTAAAGAACAGCTCCACATGAAGGAGGATAATGCAGAAGAATGTTCATTTCCATTCAAAGCGGCAACAGATGAAAATTCAGACATTCAAGTTATGAGGTTGAAAAGTGAGCACCAGAAGAATGTGCTATCAGAATTGACCAAGGATTTGCAGGCTGAGTCAGTGTCCGCTACAATGGCAGATACTCAGAAGCATGAGGATGGGATTCTAGTGGAAGAGCTAAGAATTACAAAGGAAAAACTTGAGAACTCGCAGAAAGAACATTCTAAATTAAAATTTGAACTTGAGAACAATAAAGCACCAGAGAAAATCTGCCATTTGCCGGATGAGCTTGAAGCTGCTCGAAAAGATACTGACACATGGAAGGACAAGGTGAGTGCAGATAGAAGAGCGGTCGCCGAGCTCCAAGAAAGAATTTCAAGGTTGAAAGCTAGAGATCTTAAACTTGCAGTATCTAATGCTGAGCATAAAATTTTTCCTGAGAATGTAACGATTACGGCTGAAATATCTCAGCCACCTAAGGAACAAGCTCTGTTGGAGGATGAGATCAGAAAAGTCAAGACAGAAAAGGAGGATCTGAAGGAGAGACTTAACCGCGAGATTGAGAATCTTATAAGTGGCCACAACGGCTTCCAATCTGAAAGAGATCAGCTCCACGCAGAACTTATTACTTTAAAAGAGAATTTGAGCTCTAAAGGCAAGCTTGTGCAAAAATTGGAAAGGGAACGTGTGGAACTAGAAGGGAAGGTGGAGAGGCAAAGAGTAGTGATAATGGAAGGAGCAGAGGAAAAAAGAGAGGCTATAAGGCAACTATGTTTTTCAATTGAGCATTACAGGAGTGGATATCATATGCTTAGAGAAGTTTTTATCAGTCAAAAGCGGGATCTCGGGTTCGCATCTCAAGGCAAGTCATGA

Protein sequence

MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATDENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEKREAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQGKS
Homology
BLAST of Cp4.1LG02g11400 vs. ExPASy Swiss-Prot
Match: F4KEW8 (Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.4e-61
Identity = 212/572 (37.06%), Postives = 293/572 (51.22%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           +MDRS+K M++LIEEDADSFAKKAEMYYQ RP +I           +LV+          
Sbjct: 43  EMDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELI-----------ALVD---------- 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
                                  EF+  YR+L ERY+++TGELR+  P +LQS GS + D
Sbjct: 103 -----------------------EFHRMYRALAERYENITGELRKGSPLELQSQGSGLSD 162

Query: 123 LI--------------------SEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPG 182
           +                     S  RA GF+ FLG+GG  SD   K+G DS+  TDSE  
Sbjct: 163 ISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYHKDGDDSASITDSELE 222

Query: 183 SDDSSVYNYSDGEXEGYDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQ 242
           SDDSSV NY      GY S        GSD          Q L++++M+LE ELREAKE+
Sbjct: 223 SDDSSVTNY-----PGYVSI-------GSD---------FQSLSKRIMDLEIELREAKER 282

Query: 243 LHMK-EDNAEE-----------CSFPFKAATDE-----------NSDIQVMRLKSEHQKN 302
           L M+ E N E              FP K A  E           NS+ Q+  LKS+  + 
Sbjct: 283 LRMQLEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARY 342

Query: 303 VLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENSQKEHSKLKFELENNKAP 362
           + S L  D Q+E      A TQ+ +   L EELRIT  +L  ++K++  ++ E+E +K+ 
Sbjct: 343 LPSGL-DDEQSEGA----ASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSD 402

Query: 363 E-KICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA---------RDLKLAV 422
           + K+  L D LE+A+K+   WK K SAD+R V +L +RIS LK+         RDLK A+
Sbjct: 403 DAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDHEIRDLKTAL 462

Query: 423 SNAEHKIFPENVTITAEISQPPKEQ--------------ALLEDEIRKVKTEKEDLKERL 482
           S+AE KIFPE   + A+I++  +E+                LEDE RKV  EK + +E+L
Sbjct: 463 SDAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELEANVRYLEDERRKVNNEKIEEEEKL 522

Query: 483 NREIENLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVI 508
             EIE L           + L  ++  L+  +S  G  ++  +   +E+E +VE+QR  +
Sbjct: 523 KSEIEVLTLEKVEKGRCIETLSRKVSELESEISRLGSEIKARDDRTMEMEKEVEKQRREL 544

BLAST of Cp4.1LG02g11400 vs. ExPASy Swiss-Prot
Match: Q84VY2 (Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 5.4e-50
Identity = 188/552 (34.06%), Postives = 262/552 (47.46%), Query Frame = 0

Query: 4   MDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELRR 63
           MD  + HML+LIEEDADSFAKKA+MY+QKRP +I            LVE           
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELI-----------QLVE----------- 103

Query: 64  NXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICDL 123
                                 EFY  YR+L ERYD  +GEL++N  S++QS  S     
Sbjct: 104 ----------------------EFYRMYRALAERYDQASGELQKNHTSEIQSQSS----- 163

Query: 124 ISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSSF 183
                                              S P  +  S    S  E E  DSS 
Sbjct: 164 --------------------------------LEISSPTKEKLSRRQSSHKEEE--DSSS 223

Query: 184 FTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAE---ECSFPFKAA 243
            TDS   SD SS    DGD+ L R+M ELE EL+E K++L +++++ +         K  
Sbjct: 224 LTDSGSDSDHSSANDEDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLHKIT 283

Query: 244 TDENS-----------DIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGI-- 303
           T E             + ++  LK++ Q  +  +    L AE  S  +      ED +  
Sbjct: 284 TYEGELKEANEKMRMHEDEIANLKNQLQSFMSFDTEDHLGAEQKSVDLDKEDTKEDAVAT 343

Query: 304 ----LVEELRITKEKLENSQKEHSKLKFELENNK-APEKICHLPDELEAARKDTDTWKDK 363
               L EEL I KEKL++ +KE   LK ELE  K A EK+  L  ELE A++D DT+ +K
Sbjct: 344 KVLALEEELSIAKEKLQHFEKETYSLKNELEIGKAAEEKLKSLQHELELAQRDADTYINK 403

Query: 364 VSADRRAVAELQERISRLKA---------RDLKLAVSNAEHKIFPENVTITAEISQPPKE 423
           ++A+++ V +LQER++ +K          R LK AVS+AE KIFPE   I  E+S+  +E
Sbjct: 404 LNAEKKEVLKLQERLAMVKTSLQDRDNEIRALKTAVSDAEQKIFPEKAQIKGEMSKMLEE 463

Query: 424 QAL-------LEDEIRKVKTEKEDLKERLNREIENLISGHNGFQSERDQLHAELITLKEN 483
           ++        LE  IR +K EK + +E+L    E +    +G + E + L  E+   +E 
Sbjct: 464 RSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKI----SGMRDESNVLREEIGKREEK 508

Query: 484 LSSKGKLVQKLERERV-------ELEGKVERQRVVIMEGAEEKREAIRQLCFSIEHYRSG 512
           +    K +++L  E+V       EL  +VER RV   E AE+KREAIRQLC S++HYR G
Sbjct: 524 IKETEKHMEELHMEQVRLRRRSSELTEEVERTRVSASEMAEQKREAIRQLCMSLDHYRDG 508

BLAST of Cp4.1LG02g11400 vs. ExPASy Swiss-Prot
Match: Q9ZQX8 (Protein NETWORKED 1C OS=Arabidopsis thaliana OX=3702 GN=NET1C PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 3.6e-09
Identity = 115/475 (24.21%), Postives = 197/475 (41.47%), Query Frame = 0

Query: 1   MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGE 60
           + DMD ++K M++++EEDADSFA++AEMYY+KRP ++  + EFY  YR+L ERY+H TG 
Sbjct: 33  LADMDSNVKQMIKVLEEDADSFARRAEMYYRKRPELMKLVEEFYRAYRALAERYNHATGV 92

Query: 61  LRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDI 120
           +      K  E   +  P  +  I+   +   +L    D  T ++    P   ++ G   
Sbjct: 93  IH-----KAHETIAEAFPNQVPLIFGDESHGGALTNDVDPQTPDM----PPPFRARG--- 152

Query: 121 CDLISEPRAAGFDVFLGSGGSNSDTCQKE--GYDSSFFTDSEPGSDDSSVYNYSDGEXEG 180
                                N D  Q++  G+  S   D +   D S            
Sbjct: 153 ---------------------NPDEFQQDALGFSLSHVHDVKRNIDFS------------ 212

Query: 181 YDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFK 240
            +   F  +  G     + ++D   G  R  +         K+ +  + + A +      
Sbjct: 213 -EEPLFVSN--GKARKGLNFNDHGDGKGRNGL---------KDHILSESERASKAEAEVV 272

Query: 241 AATDENSDIQVMRLKS--EHQKNVLSELTKDLQAESVSATMADTQKHEDGIL-----VEE 300
           A  D  S +Q  +  S    +KN+  E   +L++E VS   AD++   D        ++ 
Sbjct: 273 ALKDSLSKMQAEKQASLALFEKNL--ERLSNLESE-VSRAQADSRGINDRAASAEAEIQT 332

Query: 301 LRITKEKLENSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAV- 360
           LR T  KLE S+KE S L++    +K  +KI  L D L  A K+      K   +  A+ 
Sbjct: 333 LRETLYKLE-SEKESSFLQY----HKCLQKIADLEDGLSVAHKEAGERASKAETETLALK 392

Query: 361 -----AELQERISRLKARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVK 420
                AE  +  + ++ R     +SN E +           + +  ++  L+ +   K  
Sbjct: 393 RSLAKAETDKETALIQYRQCLNTISNLEER-----------LRKAEEDARLINERAEKAG 427

Query: 421 TEKEDLKERLNREIENLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERE 461
            E E+LK+ +++ I++  +    FQ    Q    + +LK  L    +  Q L  E
Sbjct: 453 VEVENLKQTVSKLIKDKEASELQFQ----QCLNIIASLKVKLHHAQEETQSLSHE 427

BLAST of Cp4.1LG02g11400 vs. ExPASy Swiss-Prot
Match: F4HZB5 (Protein NETWORKED 1D OS=Arabidopsis thaliana OX=3702 GN=NET1D PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 3.0e-08
Identity = 118/516 (22.87%), Postives = 227/516 (43.99%), Query Frame = 0

Query: 1   MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGE 60
           + DMD  +K M+++IEEDADSFA++AEMYY+KRP ++  + EFY  YR+L ERYDH TG 
Sbjct: 32  LTDMDSKVKQMIKVIEEDADSFARRAEMYYKKRPELMKLVEEFYRAYRALAERYDHATGV 91

Query: 61  LRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDI 120
           + R+     AE +  + P+M        +   S  + +D  T +    I + +       
Sbjct: 92  I-RHAQQTMAEAFPNQDPMMFGE----ESPLGSSTDGFDPQTPDSYPPIRAPVYPD---- 151

Query: 121 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSV--------YNYS 180
                       D+  G+ G +S        + +F  D +  S              N++
Sbjct: 152 ------------DLRKGAFGISSSHLSTVKRNIAFMEDPQSVSSGKGFKTAKARKGLNFN 211

Query: 181 DGEXEGYDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEE 240
           + + +  ++   ++SE  S   +   +  D  L++   E E  L +  + L    +   E
Sbjct: 212 NVDGKEINAKVLSESERASKAEAEIVALKD-ALSKVQAEKEASLAQFDQNLEKLSNLESE 271

Query: 241 CSFPFKAATDENSDIQVMR-LKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEE 300
            S        E+S + + R  ++E +   L E    ++ E  S+ +   Q       ++ 
Sbjct: 272 VS-----RAQEDSRVLIERATRAEAEVETLRESLSKVEVEKESSLLQYQQ------CLQN 331

Query: 301 LRITKEKLENSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVA 360
           +   ++++  +QKE  ++  +   N+A  +   L   L ++  D +    +     + ++
Sbjct: 332 IADLEDRISLAQKEAGEV--DERANRAEAETLALKQSLVSSETDKEAALVQYQQCLKTIS 391

Query: 361 ELQERISRLKARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDL 420
            L+ER+ + +  D +L    AE+    E  ++  ++S+  +E    E + ++      DL
Sbjct: 392 NLEERLHKAE-EDSRLTNQRAEN-AEGEVESLKQKVSKLIEENEAYELQYQQCLDTIADL 451

Query: 421 K----------ERLNREIENLISGHNGFQSER--------DQLHAELITLKENLSSKGKL 480
           K          +RL+REIE+ ++    F  E+          LH+EL  L E L ++   
Sbjct: 452 KLKLFHAQEETQRLSREIEDGVAKLK-FAEEKCVVLERSNQNLHSELDGLLEKLGNQSHE 508

Query: 481 VQKLERERVELEGKVERQRVVIMEGAEEKREAIRQL 490
           + + ++E   L   V+ + +  ME AE   + ++QL
Sbjct: 512 LTEKQKELGRLWTCVQEENLRFME-AETAFQTLQQL 508

BLAST of Cp4.1LG02g11400 vs. ExPASy Swiss-Prot
Match: F4JIF4 (Protein NETWORKED 1B OS=Arabidopsis thaliana OX=3702 GN=NET1B PE=2 SV=1)

HSP 1 Score: 61.2 bits (147), Expect = 3.9e-08
Identity = 113/509 (22.20%), Postives = 215/509 (42.24%), Query Frame = 0

Query: 1   MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGE 60
           + DMD  +K M++LIE DADSFA++A+MY++KRP ++  + E Y  YR+L ERYDH T E
Sbjct: 32  LADMDSKVKTMIKLIEADADSFARRADMYFKKRPELMKLVEELYRAYRALAERYDHTTVE 91

Query: 61  LRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDI 120
           LRR                           ++ +VE + +   ++  ++  D  S     
Sbjct: 92  LRR--------------------------AHKVMVEAFPN---QMSFDMIEDSASSS--- 151

Query: 121 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYD 180
               SEPR            ++++  QK+G  S          D +S  + +D E E   
Sbjct: 152 ----SEPRTE----------ADTEALQKDGTKSKRSFSQMNKLDGTSDSHEADSEVETLK 211

Query: 181 SSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 240
            +   + +   +  ++ Y    Q +  K+   E EL +A++ +   ++ A +     K  
Sbjct: 212 RTLL-ELQTEKEALNLQY----QLILSKVSRFEKELNDAQKDVKGFDERACKADIEIKIL 271

Query: 241 TDENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 300
            +  + ++V R     Q +   E   DL+A S+S      +   + +   E      K E
Sbjct: 272 KESLAKLEVERDTGLLQYSQAIERIADLEA-SISHGQEYAKGLTNRVSEAEREAMSLKKE 331

Query: 301 NSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRL 360
            S+ +  K    L  NK+ E I  L   +  A +    ++D+       +  L++ + +L
Sbjct: 332 LSRLQSEKEAGLLRYNKSLELISSLEKTIRDAEESVRVFRDQSEQAETEIKALKQELLKL 391

Query: 361 K--------------------ARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDE 420
                                 R++  A  NA+ ++  E +   A+I    ++ ALLE  
Sbjct: 392 NEVNEDLNVRYQQCLETISKLEREVSHAQDNAK-RLSSEVLAGAAKIKTVEEQCALLESF 451

Query: 421 IRKVKTEKEDLKERLNREIENLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERE 480
            + +K E E+L  +++ + + L    +  Q+E ++L A +   +   S  G  ++ LE  
Sbjct: 452 NQTMKVEAENLAHKMSAKDQEL----SQKQNEIEKLQAVMQEEQLRFSELGASLRNLE-- 479

Query: 481 RVELEGKVERQRVVIMEGAEEKREAIRQL 490
              L  + + ++ V+      + + +R+L
Sbjct: 512 --SLHSQSQEEQKVLTSELHSRIQMLREL 479

BLAST of Cp4.1LG02g11400 vs. NCBI nr
Match: XP_023522537.1 (protein NETWORKED 4A-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 811 bits (2095), Expect = 6.15e-293
Identity = 448/520 (86.15%), Postives = 449/520 (86.35%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR
Sbjct: 43  DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
           RN                                            +PSDLQSHGSDICD
Sbjct: 103 RN--------------------------------------------LPSDLQSHGSDICD 162

Query: 123 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSS 182
           LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGE       
Sbjct: 163 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGE------- 222

Query: 183 FFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 242
                               QGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD
Sbjct: 223 --------------------QGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 282

Query: 243 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 302
           ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS
Sbjct: 283 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 342

Query: 303 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 362
           QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA
Sbjct: 343 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 402

Query: 363 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 422
           RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL
Sbjct: 403 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 462

Query: 423 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 482
           ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK
Sbjct: 463 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 491

Query: 483 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQGKS 522
           REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQGKS
Sbjct: 523 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQGKS 491

BLAST of Cp4.1LG02g11400 vs. NCBI nr
Match: XP_023522519.1 (protein NETWORKED 4A-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 809 bits (2090), Expect = 4.28e-292
Identity = 446/518 (86.10%), Postives = 447/518 (86.29%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR
Sbjct: 43  DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
           RN                                            +PSDLQSHGSDICD
Sbjct: 103 RN--------------------------------------------LPSDLQSHGSDICD 162

Query: 123 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSS 182
           LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY           
Sbjct: 163 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY----------- 222

Query: 183 FFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 242
                           SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD
Sbjct: 223 ----------------SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 282

Query: 243 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 302
           ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS
Sbjct: 283 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 342

Query: 303 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 362
           QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA
Sbjct: 343 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 402

Query: 363 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 422
           RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL
Sbjct: 403 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 462

Query: 423 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 482
           ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK
Sbjct: 463 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 489

Query: 483 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 520
           REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG
Sbjct: 523 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 489

BLAST of Cp4.1LG02g11400 vs. NCBI nr
Match: XP_023521179.1 (protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023522268.1 protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023522526.1 protein NETWORKED 4A-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 808 bits (2088), Expect = 8.63e-292
Identity = 447/518 (86.29%), Postives = 447/518 (86.29%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           DMDRSIKHMLRLIEEDADSF                                        
Sbjct: 43  DMDRSIKHMLRLIEEDADSF---------------------------------------- 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
               AKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD
Sbjct: 103 ----AKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 162

Query: 123 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSS 182
           LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGE       
Sbjct: 163 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGE------- 222

Query: 183 FFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 242
                               QGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD
Sbjct: 223 --------------------QGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 282

Query: 243 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 302
           ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS
Sbjct: 283 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 342

Query: 303 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 362
           QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA
Sbjct: 343 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 402

Query: 363 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 422
           RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL
Sbjct: 403 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 462

Query: 423 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 482
           ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK
Sbjct: 463 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 489

Query: 483 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 520
           REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG
Sbjct: 523 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 489

BLAST of Cp4.1LG02g11400 vs. NCBI nr
Match: XP_023522532.1 (protein NETWORKED 4A-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 808 bits (2087), Expect = 1.18e-291
Identity = 445/518 (85.91%), Postives = 446/518 (86.10%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR
Sbjct: 43  DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
           RN                                            +PSDLQSHGSDICD
Sbjct: 103 RN--------------------------------------------LPSDLQSHGSDICD 162

Query: 123 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSS 182
           LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDS                       
Sbjct: 163 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDS----------------------- 222

Query: 183 FFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 242
                EPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD
Sbjct: 223 -----EPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 282

Query: 243 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 302
           ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS
Sbjct: 283 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 342

Query: 303 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 362
           QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA
Sbjct: 343 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 402

Query: 363 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 422
           RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL
Sbjct: 403 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 462

Query: 423 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 482
           ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK
Sbjct: 463 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 488

Query: 483 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 520
           REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG
Sbjct: 523 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 488

BLAST of Cp4.1LG02g11400 vs. NCBI nr
Match: XP_022997716.1 (protein NETWORKED 4A-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 769 bits (1986), Expect = 2.40e-276
Identity = 427/522 (81.80%), Postives = 436/522 (83.52%), Query Frame = 0

Query: 1   MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGE 60
           + DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFY+TYRSLVERYDHVTGE
Sbjct: 41  LADMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYSTYRSLVERYDHVTGE 100

Query: 61  LRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDI 120
           LRRN                                            IPSDLQSHGSDI
Sbjct: 101 LRRN--------------------------------------------IPSDLQSHGSDI 160

Query: 121 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYD 180
           CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY         
Sbjct: 161 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY--------- 220

Query: 181 SSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 240
                             SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA
Sbjct: 221 ------------------SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 280

Query: 241 TDENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 300
           TDENSDIQ+MRLKSEHQKN+LSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE
Sbjct: 281 TDENSDIQIMRLKSEHQKNILSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 340

Query: 301 NSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRL 360
           NSQKEHSKLKFELENNKAPEKICHLPDELEA RKDTDTWKDKVSADRRAVAELQERISRL
Sbjct: 341 NSQKEHSKLKFELENNKAPEKICHLPDELEAVRKDTDTWKDKVSADRRAVAELQERISRL 400

Query: 361 KARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIE 420
           KARDLKLAVSNA HK FPENV I AEISQP KEQALLEDEIRK+KTEK DL+ERLNREIE
Sbjct: 401 KARDLKLAVSNAGHKFFPENVPIKAEISQPLKEQALLEDEIRKIKTEKVDLEERLNREIE 460

Query: 421 NLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAE 480
            L SG NGFQSERDQLHAE+ TLKENLSSKGKLVQKLE+ERVELEGKVERQRVVIMEGAE
Sbjct: 461 TLQSGLNGFQSERDQLHAEVTTLKENLSSKGKLVQKLEKERVELEGKVERQRVVIMEGAE 491

Query: 481 EKREAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQGKS 522
           EKREAIRQLCFSIEHYRSGYHMLREVFIS+K DL FA QGKS
Sbjct: 521 EKREAIRQLCFSIEHYRSGYHMLREVFISRKWDLVFAPQGKS 491

BLAST of Cp4.1LG02g11400 vs. ExPASy TrEMBL
Match: A0A6J1K893 (protein NETWORKED 4A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492593 PE=4 SV=1)

HSP 1 Score: 769 bits (1986), Expect = 1.16e-276
Identity = 427/522 (81.80%), Postives = 436/522 (83.52%), Query Frame = 0

Query: 1   MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGE 60
           + DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFY+TYRSLVERYDHVTGE
Sbjct: 41  LADMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYSTYRSLVERYDHVTGE 100

Query: 61  LRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDI 120
           LRRN                                            IPSDLQSHGSDI
Sbjct: 101 LRRN--------------------------------------------IPSDLQSHGSDI 160

Query: 121 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYD 180
           CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY         
Sbjct: 161 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY--------- 220

Query: 181 SSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 240
                             SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA
Sbjct: 221 ------------------SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 280

Query: 241 TDENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 300
           TDENSDIQ+MRLKSEHQKN+LSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE
Sbjct: 281 TDENSDIQIMRLKSEHQKNILSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 340

Query: 301 NSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRL 360
           NSQKEHSKLKFELENNKAPEKICHLPDELEA RKDTDTWKDKVSADRRAVAELQERISRL
Sbjct: 341 NSQKEHSKLKFELENNKAPEKICHLPDELEAVRKDTDTWKDKVSADRRAVAELQERISRL 400

Query: 361 KARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIE 420
           KARDLKLAVSNA HK FPENV I AEISQP KEQALLEDEIRK+KTEK DL+ERLNREIE
Sbjct: 401 KARDLKLAVSNAGHKFFPENVPIKAEISQPLKEQALLEDEIRKIKTEKVDLEERLNREIE 460

Query: 421 NLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAE 480
            L SG NGFQSERDQLHAE+ TLKENLSSKGKLVQKLE+ERVELEGKVERQRVVIMEGAE
Sbjct: 461 TLQSGLNGFQSERDQLHAEVTTLKENLSSKGKLVQKLEKERVELEGKVERQRVVIMEGAE 491

Query: 481 EKREAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQGKS 522
           EKREAIRQLCFSIEHYRSGYHMLREVFIS+K DL FA QGKS
Sbjct: 521 EKREAIRQLCFSIEHYRSGYHMLREVFISRKWDLVFAPQGKS 491

BLAST of Cp4.1LG02g11400 vs. ExPASy TrEMBL
Match: A0A6J1GAV6 (protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111452470 PE=4 SV=1)

HSP 1 Score: 767 bits (1981), Expect = 8.07e-276
Identity = 426/518 (82.24%), Postives = 436/518 (84.17%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFY+TYRSLVERYDHVTGELR
Sbjct: 43  DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYSTYRSLVERYDHVTGELR 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
           RN                                            +PSDLQSHGSDICD
Sbjct: 103 RN--------------------------------------------LPSDLQSHGSDICD 162

Query: 123 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSS 182
           LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY           
Sbjct: 163 LISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY----------- 222

Query: 183 FFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 242
                           SDGDQ LNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD
Sbjct: 223 ----------------SDGDQELNRKMMELENELREAKEQLHMKEDNAEECSFPFKAATD 282

Query: 243 ENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENS 302
           ENSDIQ+MRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILV+ELRITK+KLENS
Sbjct: 283 ENSDIQIMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVKELRITKQKLENS 342

Query: 303 QKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA 362
            KEHSKLKFELENNK PEKICHLPDELEAARKDT+TWKDKVSADRRAVAELQERISRLKA
Sbjct: 343 HKEHSKLKFELENNKEPEKICHLPDELEAARKDTETWKDKVSADRRAVAELQERISRLKA 402

Query: 363 RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIENL 422
           RDLKLAVSNAEH IFPENV I AEISQP KEQALLEDEIRKV+TEKEDL+ERLNREIENL
Sbjct: 403 RDLKLAVSNAEHNIFPENVPIKAEISQPLKEQALLEDEIRKVQTEKEDLEERLNREIENL 462

Query: 423 ISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 482
            SG NGFQSERDQLHAE+ITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK
Sbjct: 463 QSGLNGFQSERDQLHAEVITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEK 489

Query: 483 REAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 520
           REAIRQLCFSIEHYRSGYHMLREV IS+KRDL FASQG
Sbjct: 523 REAIRQLCFSIEHYRSGYHMLREVVISRKRDLKFASQG 489

BLAST of Cp4.1LG02g11400 vs. ExPASy TrEMBL
Match: A0A6J1KAQ6 (protein NETWORKED 4A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492593 PE=4 SV=1)

HSP 1 Score: 766 bits (1977), Expect = 4.41e-275
Identity = 425/520 (81.73%), Postives = 434/520 (83.46%), Query Frame = 0

Query: 1   MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGE 60
           + DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFY+TYRSLVERYDHVTGE
Sbjct: 41  LADMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYSTYRSLVERYDHVTGE 100

Query: 61  LRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDI 120
           LRRN                                            IPSDLQSHGSDI
Sbjct: 101 LRRN--------------------------------------------IPSDLQSHGSDI 160

Query: 121 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYD 180
           CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY         
Sbjct: 161 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY--------- 220

Query: 181 SSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 240
                             SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA
Sbjct: 221 ------------------SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 280

Query: 241 TDENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 300
           TDENSDIQ+MRLKSEHQKN+LSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE
Sbjct: 281 TDENSDIQIMRLKSEHQKNILSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 340

Query: 301 NSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRL 360
           NSQKEHSKLKFELENNKAPEKICHLPDELEA RKDTDTWKDKVSADRRAVAELQERISRL
Sbjct: 341 NSQKEHSKLKFELENNKAPEKICHLPDELEAVRKDTDTWKDKVSADRRAVAELQERISRL 400

Query: 361 KARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIE 420
           KARDLKLAVSNA HK FPENV I AEISQP KEQALLEDEIRK+KTEK DL+ERLNREIE
Sbjct: 401 KARDLKLAVSNAGHKFFPENVPIKAEISQPLKEQALLEDEIRKIKTEKVDLEERLNREIE 460

Query: 421 NLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAE 480
            L SG NGFQSERDQLHAE+ TLKENLSSKGKLVQKLE+ERVELEGKVERQRVVIMEGAE
Sbjct: 461 TLQSGLNGFQSERDQLHAEVTTLKENLSSKGKLVQKLEKERVELEGKVERQRVVIMEGAE 489

Query: 481 EKREAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQG 520
           EKREAIRQLCFSIEHYRSGYHMLREVFIS+K DL FA QG
Sbjct: 521 EKREAIRQLCFSIEHYRSGYHMLREVFISRKWDLVFAPQG 489

BLAST of Cp4.1LG02g11400 vs. ExPASy TrEMBL
Match: A0A6J1KEQ8 (protein NETWORKED 4A-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492593 PE=4 SV=1)

HSP 1 Score: 763 bits (1971), Expect = 2.15e-274
Identity = 424/519 (81.70%), Postives = 433/519 (83.43%), Query Frame = 0

Query: 1   MLDMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGE 60
           + DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFY+TYRSLVERYDHVTGE
Sbjct: 41  LADMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYSTYRSLVERYDHVTGE 100

Query: 61  LRRNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDI 120
           LRRN                                            IPSDLQSHGSDI
Sbjct: 101 LRRN--------------------------------------------IPSDLQSHGSDI 160

Query: 121 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYD 180
           CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY         
Sbjct: 161 CDLISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNY--------- 220

Query: 181 SSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 240
                             SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA
Sbjct: 221 ------------------SDGDQGLNRKMMELENELREAKEQLHMKEDNAEECSFPFKAA 280

Query: 241 TDENSDIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 300
           TDENSDIQ+MRLKSEHQKN+LSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE
Sbjct: 281 TDENSDIQIMRLKSEHQKNILSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLE 340

Query: 301 NSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRL 360
           NSQKEHSKLKFELENNKAPEKICHLPDELEA RKDTDTWKDKVSADRRAVAELQERISRL
Sbjct: 341 NSQKEHSKLKFELENNKAPEKICHLPDELEAVRKDTDTWKDKVSADRRAVAELQERISRL 400

Query: 361 KARDLKLAVSNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTEKEDLKERLNREIE 420
           KARDLKLAVSNA HK FPENV I AEISQP KEQALLEDEIRK+KTEK DL+ERLNREIE
Sbjct: 401 KARDLKLAVSNAGHKFFPENVPIKAEISQPLKEQALLEDEIRKIKTEKVDLEERLNREIE 460

Query: 421 NLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAE 480
            L SG NGFQSERDQLHAE+ TLKENLSSKGKLVQKLE+ERVELEGKVERQRVVIMEGAE
Sbjct: 461 TLQSGLNGFQSERDQLHAEVTTLKENLSSKGKLVQKLEKERVELEGKVERQRVVIMEGAE 488

Query: 481 EKREAIRQLCFSIEHYRSGYHMLREVFISQKRDLGFASQ 519
           EKREAIRQLCFSIEHYRSGYHMLREVFIS+K DL FA Q
Sbjct: 521 EKREAIRQLCFSIEHYRSGYHMLREVFISRKWDLVFAPQ 488

BLAST of Cp4.1LG02g11400 vs. ExPASy TrEMBL
Match: A0A6J1K5V2 (protein NETWORKED 4A-like OS=Cucurbita maxima OX=3661 GN=LOC111492590 PE=4 SV=1)

HSP 1 Score: 496 bits (1277), Expect = 3.55e-167
Identity = 345/648 (53.24%), Postives = 386/648 (59.57%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           +MDRSIK ML+LIEEDADSF                                        
Sbjct: 43  EMDRSIKRMLKLIEEDADSF---------------------------------------- 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
               AKKAEMYYQKRPV+IS++ EFY  YRSL ERYDHVTGELR+NIPSDLQS GS ICD
Sbjct: 103 ----AKKAEMYYQKRPVLISHVEEFYRMYRSLAERYDHVTGELRKNIPSDLQSQGSGICD 162

Query: 123 LISEP------------------RAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSD 182
           L SEP                  RAAGFD FLGSGGSN D CQKEG +SS  T+SEP SD
Sbjct: 163 LPSEPSSTWPSPDQRLGRRRSGPRAAGFDFFLGSGGSN-DICQKEGDESSSLTESEPDSD 222

Query: 183 DSSVYNYSDGEXEGYDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLH 242
           DSSVYNY                           + GDQGLNRKM+ELE ELREA+E++ 
Sbjct: 223 DSSVYNY---------------------------AGGDQGLNRKMIELEIELREAREKIR 282

Query: 243 MKEDNAEECSFPFKAATDENSD----------------------IQVMRLKSEHQKNVLS 302
           MKE+NAE  SFPFK  TDENSD                      IQ+ RLK++  K   S
Sbjct: 283 MKEENAE-SSFPFKGLTDENSDDAFARNREEELRNANEKLRISDIQITRLKNKLLKYEQS 342

Query: 303 ELTKDLQAESVSATMADTQKHEDGI---------------------------LVEELRIT 362
           ELTKDL+AESVSATMADT++HED +                           LV EL+IT
Sbjct: 343 ELTKDLKAESVSATMADTKRHEDEVPLVINLESEVEEHHTGLEDDQVINVQGLVAELKIT 402

Query: 363 KEKLENSQKEHSKLKFELENNKAPEKICHLPDELEAARKDTDTWKDKVSADRRAVAELQE 422
           KE+LENSQKE  KLK + ENN++PEKICHL DELEAARKDT TWK K+SA++R V++LQE
Sbjct: 403 KERLENSQKELCKLKLQHENNRSPEKICHLQDELEAARKDTATWKAKLSAEKREVSKLQE 462

Query: 423 RISRLKA---------RDLKLAVSNAEHKIFPENVTITAEISQPPKEQALL--------- 482
           RI RLKA         RDL LAVS+AE KIFPE   I AEIS+  +E+ALL         
Sbjct: 463 RIVRLKASLLDRDHEIRDLNLAVSDAEQKIFPEKTQIKAEISRLLEERALLMEQLRKWEH 522

Query: 483 -----EDEIRKVKTEKEDLKERLNREI--------------ENLISGHNGFQSERDQLHA 518
                EDEIRKVK EK  L+ERLNREI              ENL SG +GFQSERDQLH+
Sbjct: 523 QTRLLEDEIRKVKREKVVLEERLNREIQLLETTIVNKVECIENLKSGLDGFQSERDQLHS 582

BLAST of Cp4.1LG02g11400 vs. TAIR 10
Match: AT5G58320.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 238.8 bits (608), Expect = 9.8e-63
Identity = 212/572 (37.06%), Postives = 293/572 (51.22%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           +MDRS+K M++LIEEDADSFAKKAEMYYQ RP +I           +LV+          
Sbjct: 43  EMDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELI-----------ALVD---------- 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
                                  EF+  YR+L ERY+++TGELR+  P +LQS GS + D
Sbjct: 103 -----------------------EFHRMYRALAERYENITGELRKGSPLELQSQGSGLSD 162

Query: 123 LI--------------------SEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPG 182
           +                     S  RA GF+ FLG+GG  SD   K+G DS+  TDSE  
Sbjct: 163 ISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYHKDGDDSASITDSELE 222

Query: 183 SDDSSVYNYSDGEXEGYDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQ 242
           SDDSSV NY      GY S        GSD          Q L++++M+LE ELREAKE+
Sbjct: 223 SDDSSVTNY-----PGYVSI-------GSD---------FQSLSKRIMDLEIELREAKER 282

Query: 243 LHMK-EDNAEE-----------CSFPFKAATDE-----------NSDIQVMRLKSEHQKN 302
           L M+ E N E              FP K A  E           NS+ Q+  LKS+  + 
Sbjct: 283 LRMQLEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARY 342

Query: 303 VLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENSQKEHSKLKFELENNKAP 362
           + S L  D Q+E      A TQ+ +   L EELRIT  +L  ++K++  ++ E+E +K+ 
Sbjct: 343 LPSGL-DDEQSEGA----ASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSD 402

Query: 363 E-KICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA---------RDLKLAV 422
           + K+  L D LE+A+K+   WK K SAD+R V +L +RIS LK+         RDLK A+
Sbjct: 403 DAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDHEIRDLKTAL 462

Query: 423 SNAEHKIFPENVTITAEISQPPKEQ--------------ALLEDEIRKVKTEKEDLKERL 482
           S+AE KIFPE   + A+I++  +E+                LEDE RKV  EK + +E+L
Sbjct: 463 SDAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELEANVRYLEDERRKVNNEKIEEEEKL 522

Query: 483 NREIENLISGHNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVI 508
             EIE L           + L  ++  L+  +S  G  ++  +   +E+E +VE+QR  +
Sbjct: 523 KSEIEVLTLEKVEKGRCIETLSRKVSELESEISRLGSEIKARDDRTMEMEKEVEKQRREL 544

BLAST of Cp4.1LG02g11400 vs. TAIR 10
Match: AT5G58320.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 215.3 bits (547), Expect = 1.2e-55
Identity = 199/562 (35.41%), Postives = 284/562 (50.53%), Query Frame = 0

Query: 3   DMDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELR 62
           +MDRS+K M++LIEEDADSFAKKAEMYYQ RP +I           +LV+          
Sbjct: 43  EMDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELI-----------ALVD---------- 102

Query: 63  RNXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICD 122
                                  EF+  YR+L ERY+++TGELR+  P +LQS GS + D
Sbjct: 103 -----------------------EFHRMYRALAERYENITGELRKGSPLELQSQGSGLSD 162

Query: 123 LI--------------------SEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPG 182
           +                     S  RA GF+ FLG+GG  SD   K+G DS+  TDSE  
Sbjct: 163 ISASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYHKDGDDSASITDSELE 222

Query: 183 SDDSSVYNYSDGEXEGYDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQ 242
           SDDSSV NY      GY S        GSD          Q L++++M+LE ELREAKE+
Sbjct: 223 SDDSSVTNY-----PGYVSI-------GSD---------FQSLSKRIMDLEIELREAKER 282

Query: 243 LHMK-EDNAEE-----------CSFPFKAATDE-----------NSDIQVMRLKSEHQKN 302
           L M+ E N E              FP K A  E           NS+ Q+  LKS+  + 
Sbjct: 283 LRMQLEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARY 342

Query: 303 VLSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENSQKEHSKLKFELENNKAP 362
           + S L  D Q+E      A TQ+ +   L EELRIT  +L  ++K++  ++ E+E +K+ 
Sbjct: 343 LPSGL-DDEQSEGA----ASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSD 402

Query: 363 E-KICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA---------RDLKLAV 422
           + K+  L D LE+A+K+   WK K SAD+R V +L +RIS LK+         RDLK A+
Sbjct: 403 DAKLKSLQDMLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDHEIRDLKTAL 462

Query: 423 SNAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTE---KEDLKERLNRE-IENLISG 482
           S+AE KIFPE   + A+I++  +E+   +D+ ++++      ED + ++N E IE     
Sbjct: 463 SDAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELEANVRYLEDERRKVNNEKIE----- 511

Query: 483 HNGFQSERDQLHAELITLKENLSSKGKLVQKLERERVELEGKVERQRVVIMEGAEEKREA 508
                 E ++L +E+  L      KG+ ++ L R+  ELE ++ R  +            
Sbjct: 523 ------EEEKLKSEIEVLTLEKVEKGRCIETLSRKVSELESEISRLGM------------ 511

BLAST of Cp4.1LG02g11400 vs. TAIR 10
Match: AT5G58320.3 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-51
Identity = 187/524 (35.69%), Postives = 267/524 (50.95%), Query Frame = 0

Query: 4   MDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELRR 63
           MDRS+K M++LIEEDADSFAKKAEMYYQ RP +I           +LV+           
Sbjct: 1   MDRSVKRMVKLIEEDADSFAKKAEMYYQSRPELI-----------ALVD----------- 60

Query: 64  NXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICDL 123
                                 EF+  YR+L ERY+++TGELR+  P +LQS GS + D+
Sbjct: 61  ----------------------EFHRMYRALAERYENITGELRKGSPLELQSQGSGLSDI 120

Query: 124 I--------------------SEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGS 183
                                S  RA GF+ FLG+GG  SD   K+G DS+  TDSE  S
Sbjct: 121 SASDLSALWTSNEVNRLGRPPSGRRAPGFEYFLGNGGLPSDLYHKDGDDSASITDSELES 180

Query: 184 DDSSVYNYSDGEXEGYDSSFFTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQL 243
           DDSSV NY      GY S        GSD          Q L++++M+LE ELREAKE+L
Sbjct: 181 DDSSVTNY-----PGYVSI-------GSD---------FQSLSKRIMDLEIELREAKERL 240

Query: 244 HMK-EDNAEE-----------CSFPFKAATDE-----------NSDIQVMRLKSEHQKNV 303
            M+ E N E              FP K A  E           NS+ Q+  LKS+  + +
Sbjct: 241 RMQLEGNTESLLPRVKSETKFVDFPAKLAACEQELKDVNEKLQNSEDQIYILKSQLARYL 300

Query: 304 LSELTKDLQAESVSATMADTQKHEDGILVEELRITKEKLENSQKEHSKLKFELENNKAPE 363
            S L  D Q+E      A TQ+ +   L EELRIT  +L  ++K++  ++ E+E +K+ +
Sbjct: 301 PSGL-DDEQSEGA----ASTQELDIETLSEELRITSLRLREAEKQNGIMRKEVEKSKSDD 360

Query: 364 -KICHLPDELEAARKDTDTWKDKVSADRRAVAELQERISRLKA---------RDLKLAVS 423
            K+  L D LE+A+K+   WK K SAD+R V +L +RIS LK+         RDLK A+S
Sbjct: 361 AKLKSLQDMLESAQKEAAAWKSKASADKREVVKLLDRISMLKSSLAGRDHEIRDLKTALS 420

Query: 424 NAEHKIFPENVTITAEISQPPKEQALLEDEIRKVKTE---KEDLKERLNRE-IENLISGH 471
           +AE KIFPE   + A+I++  +E+   +D+ ++++      ED + ++N E IE      
Sbjct: 421 DAEEKIFPEKAQVKADIAKLLEEKIHRDDQFKELEANVRYLEDERRKVNNEKIE------ 443

BLAST of Cp4.1LG02g11400 vs. TAIR 10
Match: AT2G30500.1 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 200.3 bits (508), Expect = 3.8e-51
Identity = 188/552 (34.06%), Postives = 262/552 (47.46%), Query Frame = 0

Query: 4   MDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELRR 63
           MD  + HML+LIEEDADSFAKKA+MY+QKRP +I            LVE           
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELI-----------QLVE----------- 103

Query: 64  NXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICDL 123
                                 EFY  YR+L ERYD  +GEL++N  S++QS  S     
Sbjct: 104 ----------------------EFYRMYRALAERYDQASGELQKNHTSEIQSQSS----- 163

Query: 124 ISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSSF 183
                                              S P  +  S    S  E E  DSS 
Sbjct: 164 --------------------------------LEISSPTKEKLSRRQSSHKEEE--DSSS 223

Query: 184 FTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAE---ECSFPFKAA 243
            TDS   SD SS    DGD+ L R+M ELE EL+E K++L +++++ +         K  
Sbjct: 224 LTDSGSDSDHSSANDEDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLHKIT 283

Query: 244 TDENS-----------DIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGI-- 303
           T E             + ++  LK++ Q  +  +    L AE  S  +      ED +  
Sbjct: 284 TYEGELKEANEKMRMHEDEIANLKNQLQSFMSFDTEDHLGAEQKSVDLDKEDTKEDAVAT 343

Query: 304 ----LVEELRITKEKLENSQKEHSKLKFELENNK-APEKICHLPDELEAARKDTDTWKDK 363
               L EEL I KEKL++ +KE   LK ELE  K A EK+  L  ELE A++D DT+ +K
Sbjct: 344 KVLALEEELSIAKEKLQHFEKETYSLKNELEIGKAAEEKLKSLQHELELAQRDADTYINK 403

Query: 364 VSADRRAVAELQERISRLKA---------RDLKLAVSNAEHKIFPENVTITAEISQPPKE 423
           ++A+++ V +LQER++ +K          R LK AVS+AE KIFPE   I  E+S+  +E
Sbjct: 404 LNAEKKEVLKLQERLAMVKTSLQDRDNEIRALKTAVSDAEQKIFPEKAQIKGEMSKMLEE 463

Query: 424 QAL-------LEDEIRKVKTEKEDLKERLNREIENLISGHNGFQSERDQLHAELITLKEN 483
           ++        LE  IR +K EK + +E+L    E +    +G + E + L  E+   +E 
Sbjct: 464 RSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKI----SGMRDESNVLREEIGKREEK 508

Query: 484 LSSKGKLVQKLERERV-------ELEGKVERQRVVIMEGAEEKREAIRQLCFSIEHYRSG 512
           +    K +++L  E+V       EL  +VER RV   E AE+KREAIRQLC S++HYR G
Sbjct: 524 IKETEKHMEELHMEQVRLRRRSSELTEEVERTRVSASEMAEQKREAIRQLCMSLDHYRDG 508

BLAST of Cp4.1LG02g11400 vs. TAIR 10
Match: AT2G30500.2 (Kinase interacting (KIP1-like) family protein )

HSP 1 Score: 200.3 bits (508), Expect = 3.8e-51
Identity = 188/552 (34.06%), Postives = 262/552 (47.46%), Query Frame = 0

Query: 4   MDRSIKHMLRLIEEDADSFAKKAEMYYQKRPMMISYIWEFYTTYRSLVERYDHVTGELRR 63
           MD  + HML+LIEEDADSFAKKA+MY+QKRP +I            LVE           
Sbjct: 44  MDDRVNHMLKLIEEDADSFAKKAQMYFQKRPELI-----------QLVE----------- 103

Query: 64  NXFAKKAEMYYQKRPVMISYIWEFYTTYRSLVERYDHVTGELRRNIPSDLQSHGSDICDL 123
                                 EFY  YR+L ERYD  +GEL++N  S++QS  S     
Sbjct: 104 ----------------------EFYRMYRALAERYDQASGELQKNHTSEIQSQSS----- 163

Query: 124 ISEPRAAGFDVFLGSGGSNSDTCQKEGYDSSFFTDSEPGSDDSSVYNYSDGEXEGYDSSF 183
                                              S P  +  S    S  E E  DSS 
Sbjct: 164 --------------------------------LEISSPTKEKLSRRQSSHKEEE--DSSS 223

Query: 184 FTDSEPGSDDSSVYYSDGDQGLNRKMMELENELREAKEQLHMKEDNAE---ECSFPFKAA 243
            TDS   SD SS    DGD+ L R+M ELE EL+E K++L +++++ +         K  
Sbjct: 224 LTDSGSDSDHSSANDEDGDEALIRRMAELELELQETKQKLLLQQESVDGDNNVDLLHKIT 283

Query: 244 TDENS-----------DIQVMRLKSEHQKNVLSELTKDLQAESVSATMADTQKHEDGI-- 303
           T E             + ++  LK++ Q  +  +    L AE  S  +      ED +  
Sbjct: 284 TYEGELKEANEKMRMHEDEIANLKNQLQSFMSFDTEDHLGAEQKSVDLDKEDTKEDAVAT 343

Query: 304 ----LVEELRITKEKLENSQKEHSKLKFELENNK-APEKICHLPDELEAARKDTDTWKDK 363
               L EEL I KEKL++ +KE   LK ELE  K A EK+  L  ELE A++D DT+ +K
Sbjct: 344 KVLALEEELSIAKEKLQHFEKETYSLKNELEIGKAAEEKLKSLQHELELAQRDADTYINK 403

Query: 364 VSADRRAVAELQERISRLKA---------RDLKLAVSNAEHKIFPENVTITAEISQPPKE 423
           ++A+++ V +LQER++ +K          R LK AVS+AE KIFPE   I  E+S+  +E
Sbjct: 404 LNAEKKEVLKLQERLAMVKTSLQDRDNEIRALKTAVSDAEQKIFPEKAQIKGEMSKMLEE 463

Query: 424 QAL-------LEDEIRKVKTEKEDLKERLNREIENLISGHNGFQSERDQLHAELITLKEN 483
           ++        LE  IR +K EK + +E+L    E +    +G + E + L  E+   +E 
Sbjct: 464 RSQLGEQLRELESHIRLIKEEKAETEEKLRGGTEKI----SGMRDESNVLREEIGKREEK 508

Query: 484 LSSKGKLVQKLERERV-------ELEGKVERQRVVIMEGAEEKREAIRQLCFSIEHYRSG 512
           +    K +++L  E+V       EL  +VER RV   E AE+KREAIRQLC S++HYR G
Sbjct: 524 IKETEKHMEELHMEQVRLRRRSSELTEEVERTRVSASEMAEQKREAIRQLCMSLDHYRDG 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4KEW81.4e-6137.06Protein NETWORKED 4A OS=Arabidopsis thaliana OX=3702 GN=NET4A PE=2 SV=1[more]
Q84VY25.4e-5034.06Protein NETWORKED 4B OS=Arabidopsis thaliana OX=3702 GN=NET4B PE=2 SV=1[more]
Q9ZQX83.6e-0924.21Protein NETWORKED 1C OS=Arabidopsis thaliana OX=3702 GN=NET1C PE=3 SV=1[more]
F4HZB53.0e-0822.87Protein NETWORKED 1D OS=Arabidopsis thaliana OX=3702 GN=NET1D PE=3 SV=1[more]
F4JIF43.9e-0822.20Protein NETWORKED 1B OS=Arabidopsis thaliana OX=3702 GN=NET1B PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023522537.16.15e-29386.15protein NETWORKED 4A-like isoform X4 [Cucurbita pepo subsp. pepo][more]
XP_023522519.14.28e-29286.10protein NETWORKED 4A-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023521179.18.63e-29286.29protein NETWORKED 4A-like [Cucurbita pepo subsp. pepo] >XP_023522268.1 protein N... [more]
XP_023522532.11.18e-29185.91protein NETWORKED 4A-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_022997716.12.40e-27681.80protein NETWORKED 4A-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1K8931.16e-27681.80protein NETWORKED 4A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492593... [more]
A0A6J1GAV68.07e-27682.24protein NETWORKED 4A-like OS=Cucurbita moschata OX=3662 GN=LOC111452470 PE=4 SV=... [more]
A0A6J1KAQ64.41e-27581.73protein NETWORKED 4A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492593... [more]
A0A6J1KEQ82.15e-27481.70protein NETWORKED 4A-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492593... [more]
A0A6J1K5V23.55e-16753.24protein NETWORKED 4A-like OS=Cucurbita maxima OX=3661 GN=LOC111492590 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G58320.29.8e-6337.06Kinase interacting (KIP1-like) family protein [more]
AT5G58320.11.2e-5535.41Kinase interacting (KIP1-like) family protein [more]
AT5G58320.31.0e-5135.69Kinase interacting (KIP1-like) family protein [more]
AT2G30500.13.8e-5134.06Kinase interacting (KIP1-like) family protein [more]
AT2G30500.23.8e-5134.06Kinase interacting (KIP1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 287..314
NoneNo IPR availableCOILSCoilCoilcoord: 392..423
NoneNo IPR availableCOILSCoilCoilcoord: 200..227
NoneNo IPR availableCOILSCoilCoilcoord: 341..361
NoneNo IPR availableCOILSCoilCoilcoord: 438..472
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..199
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..201
NoneNo IPR availablePANTHERPTHR32258:SF21KINASE INTERACTING (KIP1-LIKE) FAMILY PROTEINcoord: 64..510
NoneNo IPR availablePANTHERPTHR32258PROTEIN NETWORKED 4Acoord: 3..57
NoneNo IPR availablePANTHERPTHR32258PROTEIN NETWORKED 4Acoord: 64..510
NoneNo IPR availablePANTHERPTHR32258:SF21KINASE INTERACTING (KIP1-LIKE) FAMILY PROTEINcoord: 3..57
IPR011684Protein Networked (NET), actin-binding (NAB) domainPFAMPF07765KIP1coord: 3..55
e-value: 1.8E-19
score: 69.6
coord: 64..98
e-value: 1.1E-9
score: 38.3
IPR011684Protein Networked (NET), actin-binding (NAB) domainPROSITEPS51774NABcoord: 1..61
score: 29.356047

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g11400.1Cp4.1LG02g11400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003779 actin binding