Cp4.1LG02g11360 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g11360
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionBZIP transcription factor
LocationCp4.1LG02: 10238148 .. 10242238 (+)
RNA-Seq ExpressionCp4.1LG02g11360
SyntenyCp4.1LG02g11360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAAAAATTAGTTAGCATTATCCACGAACAAGAAAAGAGGTTGTGGATAGAGAATTTTAGTGTCTACTACAGTAAGAGTGGCAGTTGACGCCACTAATAAATTAAGCAATTTCTACTTTCCTCTACTGTCGCCATTGCTAGCGACGCGCAAAGAAGAAGAACAAGAACACCGAAGAAGATGATGAAGAAGAAGACGAACCGCCGGACTAATTGCTTGCACTAATCCAACGGCAGCGCCATCTTCCTCGCACTCACAGCCCAACAATTTACAGTAGCTTCGGAAATGCAAGATCCGGCGTCAAATCCCGATTCCTCCATCTCCAACAATGCTTCTACTCCCAACATTCATCTTCCGCCGCCGCCGCCGCCGCTCTCTTCTACCAGACCCCACCACCGCAGGGCCCACTCCGAACTCACATTTCGGTTCCCTGATGACATCATGGATCTCTCTTCCTCTGATCCCTTCACTGCCGGATCCGCCGCCCTCGCTTTGGACGAGATCGGATCTGAGGACGACCTCTTTTCCACCTACATTGATGTAGACAAGCTTGGCCCCGGCACTTCTTCTGCAGATCACACCGCTAATGCTGAACCTGAGAAGACTCCCAGACCGAGGCATAGATATAGCAACTCTGTCGATGGTTCCACCTCTACCAGTGTGTTTGGGGATGTACTGGACGCCAAGAAAGCTATGCCTCCTGATAAATTAGCTGAGCTTTGGACTCTTGATCCCAAGCGCGCCAAAAGGTCTCTCTCTCTTCCTCCATTGTCTTCCTTCTTAATGCGTATTCATTCTTGATTCAACTGATTACTGGTCATGGTGAATAGATTTAATGAAACTGGACCTCTTTCTGCCTCCAAAATTCAAGCAACTTGAGCTCTAAGCATAAAAATAACCCTCACTGCACTGTTCCCAATAACTTCAGCGCTACTTCTGATATACCCTCTCCTTTAGAGATTTGAACAATGAGCCCTTACTTTTAAGGGTGGACCCATTGTAGCGTATAGATGTGGCTCAAATCTGGTCAGAGTAATGAAGGAAGAGAAAGGGACGTGCTCAATTTAGGTTCATATCAATTGAGCAATTGCTAAACTAGATTCTAGGGAGAGTTGAGAAGGGAATTTAGAAAGGGTTTGAAGAAGCCCAGATCCTGCAGGTAGTCATATTAGAGAAGAGGAAACAATTATCAAGGGAAAGGAGAAACTTAAGGGATATGGGTATGCTATAATTCTTGCTTTTTGACCAGAAAAACTCTCTTATTGATGTCATTGAAGACACTTACCCTAATTAATGCCGCCTATGCAAGTCTAGTGATGAAACTCAGAGCTATCTTTCCTTGTCGTTATGTCTGGTGGTTCTGTTGTATTATTTTCAGCTCCTTTGACTGGACCAATCTTTATGAATGGCATCATGCTCTTTTTACTCTCATTTTGGATACTCATCCTTCAAAAGGGAGACAAAGATTAATCTGTCCAACTTTCTTGAGAGATTTTTTTCCTTTTTGTCTGCTTGGATGGAGAGGATAGAAGAATGTTCTTGAGGTTTTCTGGGAGACTTTACTTTTTCCTTGTTGCTTCTAGCAAATGGAATTATAGGTATGGTTCTTTTTACACTTGTTGGAAGACCTTTTGGAGATTTCCTTAGCTTGGGTTTTTTCTTTTTAATTTCATTATGTCGATAAGTGTATCTTATGAGAAGCTGAAGGAACGGCGTACCTTTTACATGATGGGTCAGCGAGAAAATGGGAGCAGCGGCTTAAGCTATTAGGTGTAGGCGCTTTCGACTAAGTTTGGTTTTCATGGAAGTAGAAATTTTGAGAATGGGATGGTTTAATAATCAAAGGTTTTGGGAAGTTGTGTGTGGGAGAGATCAAAAGACAAAGCATAGAGGAAAAACATGTATCCACTTGCATCTATGGTCTACACACCTATTCAGTCAAATTCTTGCCTCTACACACATTTAAAGCTTCACTTTTGCACGGATAAACAATAATGATACCAATAAATAAGTAAATTGAAGAGATATAAGAAGTTACCAGACATGTCTTCATTTTACGCATTCAGCACTTTCAACCATCTTCATTTAGGAATCTGTTCAGTGCTGTTCCTTGTGTTGTTCTTGTATAGGATTTTGGCCAATCGACAGTCTGCTTCCCGTTCTAAAGAGAGGAAGGCACGATACATGCAGGAACTCGAGCGCAAAGTTCAGACCCTTCAAACAGAGGCGACAACTCTTTCAGCTCAACTCGCACTGTTCCAGGTATGTGTTGCTAAATCAAAATTAAGCTTTCATCTTTAAAAGCTTTGGGAATTCTGAGCAAGAACCTTTTTTGAGTCTACTTAGCTCTTGGTTTATTTAACATATCAACTTATTCTCAATTCTATTGCTACTTTTTGGCTTGGAAAGTTTGATTCTGATGCATTTGTCTTCTAGATTCTTTCTGGGCTTGCATTTTAATCTCAAGAAACCAAAAAGTTACATGGTTTTGTGAGATGCTTCTACTTATGATTTCTTGTTGTTTGAGGAACTGAACTTGAACAGATTGATCCATATTTTCATAACTAGAACTTCGTATAATTTGTAACCTCATCTGACTTTCTTTTAGAATATGCGGAAAGAAAGAAATCAGAGAATATTTACAGAAAAAACATAGACCTATATAAAACTTTTCGACTATATCTTGGTGTAAACTGTCTAATATTTTTACTTCCTATAGTTATACCACCCTTCTTCCAAATTGAGAAGGTCTAATTTCAATCATCAATGAACTTGTCTCTTATTGAAAAGAAAAAGAAAAAGAAAAAGATTTTGATTTGATCTAACACTAGTCACAAGCAAGAAATTTTATTACAGTTTTCTTTTTGTCCATGCTCCTTTTCAGTTCAGACCTGCGCCATCTTAAGAATGTGTAGCCTTTCATATTATCTAACTTAGTATTAAACATGGGGCTGTTTGTATAGAGGGATACGACAGGTCTTAGTAGTGAAAATACAGAACTTAAGCTTCGGTTGCAGGCAATGGAGCAACAGGCTCATTTGCGGGATGGTAAGCACTTCCTAGATCACTTGTATCACCAAATTATCCTAAGAATTTTCCTTTCTATCATCACATGTTCTCGCCTTGGTTAATAGAAATCATCACTGTCTATGTGACACAGCCTTGAATGAAGCACTAAAGAACGAAGTTGAGAGGCTCAAAGTAGCCACTGGAGAGACATCAGGCGCCTCCGATTCCTTCAACTTGGGGGGAATGCCTCACATGCCATATTTAGCATCCAGTTTCTTTCCCCTGCATCAGCAGCCAGGTTCTGCCGGCACCCACAACATGCTGCAGATGCCATTATACGGCCATTCCTCATCAAACATGTCAATACATCCTCTGCATCATCAGCTGAATTCACATCAATTCTCTGATACCATGCAGAGTGATCCACTTGGGCGATTACAGGGTCTGGATATCAGCAGCAATGGATCCCCTCTGGTGAAATCAGAAGTCCCTTCTCTTTCTGCTAGTGAAAGTAGCGCCACATTTTGATGCAGACTGGTAGCCGGCCTGCTGATTTGCTCATGTCCAGTTCATGAGCTTGGTGTCAAATGTATTATTGTTTATAGCTCGACTTTTAGAGGCCATTTGGTGCGCTGGTTCGGAATCAGAAACACATAGGGTGGGTTGATTCGTTGGAATCCTTGAGTTTGATTAGTCTGTTCTTTGTTTTGATTTTTTTAGACTGGTTTTTCTTATCTCCATTTTTTGTGACTTGCTAATTGTTTTTGAATTGCGTAACATTATTTTGACCTATGAATCATTTGTCCCAACCTTATTCAAATCAGAATTATACTATGAATTAGTAGTTGTTCAAACATATTATTAGATTCATCATACAAAAAATTTCTATAACCTATATGAAGAGAAAGGGAAGAAGGAGTGTGTGTTTTACTTGATCTTGTAGGGAACAGTGGCGCCGCATATCTCTGAGATCGTAGTTGATTCCAGGCATTGCGGAAACGCCATTCGTAATGCAGTTCTCCGGTCAGCGGTGGTGGTGGAACCGATCGAAATCAGGCTCTTTATGGCAGAGCAGCATTCCG

mRNA sequence

AAATAAAAATTAGTTAGCATTATCCACGAACAAGAAAAGAGGTTGTGGATAGAGAATTTTAGTGTCTACTACAGTAAGAGTGGCAGTTGACGCCACTAATAAATTAAGCAATTTCTACTTTCCTCTACTGTCGCCATTGCTAGCGACGCGCAAAGAAGAAGAACAAGAACACCGAAGAAGATGATGAAGAAGAAGACGAACCGCCGGACTAATTGCTTGCACTAATCCAACGGCAGCGCCATCTTCCTCGCACTCACAGCCCAACAATTTACAGTAGCTTCGGAAATGCAAGATCCGGCGTCAAATCCCGATTCCTCCATCTCCAACAATGCTTCTACTCCCAACATTCATCTTCCGCCGCCGCCGCCGCCGCTCTCTTCTACCAGACCCCACCACCGCAGGGCCCACTCCGAACTCACATTTCGGTTCCCTGATGACATCATGGATCTCTCTTCCTCTGATCCCTTCACTGCCGGATCCGCCGCCCTCGCTTTGGACGAGATCGGATCTGAGGACGACCTCTTTTCCACCTACATTGATGTAGACAAGCTTGGCCCCGGCACTTCTTCTGCAGATCACACCGCTAATGCTGAACCTGAGAAGACTCCCAGACCGAGGCATAGATATAGCAACTCTGTCGATGGTTCCACCTCTACCAGTGTGTTTGGGGATGTACTGGACGCCAAGAAAGCTATGCCTCCTGATAAATTAGCTGAGCTTTGGACTCTTGATCCCAAGCGCGCCAAAAGGATTTTGGCCAATCGACAGTCTGCTTCCCGTTCTAAAGAGAGGAAGGCACGATACATGCAGGAACTCGAGCGCAAAGTTCAGACCCTTCAAACAGAGGCGACAACTCTTTCAGCTCAACTCGCACTGTTCCAGAGGGATACGACAGGTCTTAGTAGTGAAAATACAGAACTTAAGCTTCGGTTGCAGGCAATGGAGCAACAGGCTCATTTGCGGGATGCCTTGAATGAAGCACTAAAGAACGAAGTTGAGAGGCTCAAAGTAGCCACTGGAGAGACATCAGGCGCCTCCGATTCCTTCAACTTGGGGGGAATGCCTCACATGCCATATTTAGCATCCAGTTTCTTTCCCCTGCATCAGCAGCCAGGTTCTGCCGGCACCCACAACATGCTGCAGATGCCATTATACGGCCATTCCTCATCAAACATGTCAATACATCCTCTGCATCATCAGCTGAATTCACATCAATTCTCTGATACCATGCAGAGTGATCCACTTGGGCGATTACAGGGTCTGGATATCAGCAGCAATGGATCCCCTCTGGTGAAATCAGAAGTCCCTTCTCTTTCTGCTAGTGAAAGTAGCGCCACATTTTGATGCAGACTGGTAGCCGGCCTGCTGATTTGCTCATGTCCAGTTCATGAGCTTGGTGTCAAATGTATTATTGTTTATAGCTCGACTTTTAGAGGCCATTTGGTGCGCTGGTTCGGAATCAGAAACACATAGGGTGGGTTGATTCGTTGGAATCCTTGAGTTTGATTAGTCTGTTCTTTGTTTTGATTTTTTTAGACTGGTTTTTCTTATCTCCATTTTTTGTGACTTGCTAATTGTTTTTGAATTGCGTAACATTATTTTGACCTATGAATCATTTGTCCCAACCTTATTCAAATCAGAATTATACTATGAATTAGTAGTTGTTCAAACATATTATTAGATTCATCATACAAAAAATTTCTATAACCTATATGAAGAGAAAGGGAAGAAGGAGTGTGTGTTTTACTTGATCTTGTAGGGAACAGTGGCGCCGCATATCTCTGAGATCGTAGTTGATTCCAGGCATTGCGGAAACGCCATTCGTAATGCAGTTCTCCGGTCAGCGGTGGTGGTGGAACCGATCGAAATCAGGCTCTTTATGGCAGAGCAGCATTCCG

Coding sequence (CDS)

ATGCAAGATCCGGCGTCAAATCCCGATTCCTCCATCTCCAACAATGCTTCTACTCCCAACATTCATCTTCCGCCGCCGCCGCCGCCGCTCTCTTCTACCAGACCCCACCACCGCAGGGCCCACTCCGAACTCACATTTCGGTTCCCTGATGACATCATGGATCTCTCTTCCTCTGATCCCTTCACTGCCGGATCCGCCGCCCTCGCTTTGGACGAGATCGGATCTGAGGACGACCTCTTTTCCACCTACATTGATGTAGACAAGCTTGGCCCCGGCACTTCTTCTGCAGATCACACCGCTAATGCTGAACCTGAGAAGACTCCCAGACCGAGGCATAGATATAGCAACTCTGTCGATGGTTCCACCTCTACCAGTGTGTTTGGGGATGTACTGGACGCCAAGAAAGCTATGCCTCCTGATAAATTAGCTGAGCTTTGGACTCTTGATCCCAAGCGCGCCAAAAGGATTTTGGCCAATCGACAGTCTGCTTCCCGTTCTAAAGAGAGGAAGGCACGATACATGCAGGAACTCGAGCGCAAAGTTCAGACCCTTCAAACAGAGGCGACAACTCTTTCAGCTCAACTCGCACTGTTCCAGAGGGATACGACAGGTCTTAGTAGTGAAAATACAGAACTTAAGCTTCGGTTGCAGGCAATGGAGCAACAGGCTCATTTGCGGGATGCCTTGAATGAAGCACTAAAGAACGAAGTTGAGAGGCTCAAAGTAGCCACTGGAGAGACATCAGGCGCCTCCGATTCCTTCAACTTGGGGGGAATGCCTCACATGCCATATTTAGCATCCAGTTTCTTTCCCCTGCATCAGCAGCCAGGTTCTGCCGGCACCCACAACATGCTGCAGATGCCATTATACGGCCATTCCTCATCAAACATGTCAATACATCCTCTGCATCATCAGCTGAATTCACATCAATTCTCTGATACCATGCAGAGTGATCCACTTGGGCGATTACAGGGTCTGGATATCAGCAGCAATGGATCCCCTCTGGTGAAATCAGAAGTCCCTTCTCTTTCTGCTAGTGAAAGTAGCGCCACATTTTGA

Protein sequence

MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDGSTSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIHPLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF
Homology
BLAST of Cp4.1LG02g11360 vs. ExPASy Swiss-Prot
Match: Q6S4P4 (Transcription factor RF2b OS=Oryza sativa subsp. japonica OX=39947 GN=RF2b PE=1 SV=2)

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-80
Identity = 188/340 (55.29%), Postives = 235/340 (69.12%), Query Frame = 0

Query: 24  PPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEIGSEDDLFSTY 83
           P    P +    HHRRA SE+ FR PDD +DL        G  A A DEIGSEDDLFST+
Sbjct: 4   PKHTDPAAMRGAHHRRARSEVAFRLPDD-LDL-------GGGGAGAFDEIGSEDDLFSTF 63

Query: 84  IDVDKLGPGTSSA---DHTANAEPEKTPRPRHRYSNSVDGS--------TSTSVFGDVLD 143
           +D++K+  G ++A   D    AE    PRP+HR+S+SVDGS         + +   +V++
Sbjct: 64  MDIEKISSGPAAAGGSDRDRAAETSSPPRPKHRHSSSVDGSGFFAAARKDAAASLAEVME 123

Query: 144 AKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQTLQTEATTLS 203
           AKKAM P++L+EL  +DPKRAKRILANRQSA+RSKERKARY+ ELERKVQTLQTEATTLS
Sbjct: 124 AKKAMTPEQLSELAAIDPKRAKRILANRQSAARSKERKARYITELERKVQTLQTEATTLS 183

Query: 204 AQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVATGETSGASD 263
           AQL LFQRDTTGLS+EN ELK+RLQAMEQQA LRDALN+ALK E+ERLK+ATGE + +++
Sbjct: 184 AQLTLFQRDTTGLSAENAELKIRLQAMEQQAQLRDALNDALKQELERLKLATGEMTNSNE 243

Query: 264 SFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIHPLHHQLNSHQFS 323
           ++++ G+ H+PY  + FFPL Q   +         P +     N+  H L H    +   
Sbjct: 244 TYSM-GLQHVPY-NTPFFPLAQHNAARQNGGTQLPPQFQPPRPNVPNHMLSH---PNGLQ 303

Query: 324 DTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 353
           D MQ DPLGRLQGLDI S G  +VKSE  S+SASESS+TF
Sbjct: 304 DIMQQDPLGRLQGLDI-SKGPLVVKSESSSISASESSSTF 329

BLAST of Cp4.1LG02g11360 vs. ExPASy Swiss-Prot
Match: O22873 (bZIP transcription factor 18 OS=Arabidopsis thaliana OX=3702 GN=BZIP18 PE=1 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 8.3e-79
Identity = 206/389 (52.96%), Postives = 247/389 (63.50%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           M+DP SNP  + SN +  P +   P P P+    P+HRRAHSE+ FR P+   DL  S+P
Sbjct: 1   MEDP-SNPQPNQSNLSQCPPLATAPTPAPVRG--PYHRRAHSEVQFRLPE---DLDLSEP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKT------------- 120
           F         DE+GSEDDLF +Y+D++KLG G+ SA  +A     ++             
Sbjct: 61  FG------GFDELGSEDDLFCSYMDIEKLGSGSGSASDSAGPSAPRSDNPFSAENGGAEA 120

Query: 121 --PRPRHRYSNSVDGSTSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASR 180
              RPRHR+S SVDGS++     + ++AKKAM PDKLAELW +DPKRAKRI+ANRQSA+R
Sbjct: 121 GNSRPRHRHSLSVDGSSTL----ESIEAKKAMAPDKLAELWVVDPKRAKRIIANRQSAAR 180

Query: 181 SKERKARYMQELERKVQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHL 240
           SKERKARY+ ELERKVQTLQTEATTLSAQL+LFQRDTTGLSSENTELKLRLQ MEQQA L
Sbjct: 181 SKERKARYILELERKVQTLQTEATTLSAQLSLFQRDTTGLSSENTELKLRLQVMEQQAKL 240

Query: 241 RDALNEALKNEVERLKVATGETSGASDSFNLGGMPHMPYL---ASSFFPLHQQPGSAGTH 300
           RDALNE LK EVERLK ATGE S A D++NL GM HM Y      SFF  H Q       
Sbjct: 241 RDALNEQLKKEVERLKFATGEVSPA-DAYNL-GMAHMQYQQQPQQSFFQHHHQQ-QTDAQ 300

Query: 301 NMLQM--------PLYGHSSSNMSIHPLHHQL----------NSHQFSDTMQSDPLGRLQ 352
           N+ QM        P    + S+ +  P  HQL           SH +S+ M  D LGRLQ
Sbjct: 301 NLQQMTHQFHLFQPNNNQNQSSRTNPPTAHQLMHHATSNAPAQSHSYSEAMHEDHLGRLQ 360

BLAST of Cp4.1LG02g11360 vs. ExPASy Swiss-Prot
Match: Q69IL4 (Transcription factor RF2a OS=Oryza sativa subsp. japonica OX=39947 GN=RF2a PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 7.4e-51
Identity = 142/306 (46.41%), Postives = 182/306 (59.48%), Query Frame = 0

Query: 28  PPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVD 87
           P   +  P HRRAHSE+    P+D +DL ++     G      DE  ++++LFS ++DV+
Sbjct: 41  PDFPTRNPGHRRAHSEI-LSLPED-LDLCAAG---GGDGPSLSDE--NDEELFSMFLDVE 100

Query: 88  KL---------------GPGTSSADHTANAEPEKTPRPRHRYSNSVDGSTSTSVFGDV-- 147
           KL                 G ++A   A A      RP+H++S S+D S S      V  
Sbjct: 101 KLNSTCGASSEAEAESSSAGAAAAVAAAAAAAAHGARPKHQHSLSMDESMSIKAEELVGA 160

Query: 148 ---------LDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKV 207
                     +AKKA+   KLAEL  +DPKRAKRI ANRQSA+RSKERK RY+ ELERKV
Sbjct: 161 SPGTEGMSSAEAKKAVSAAKLAELALVDPKRAKRIWANRQSAARSKERKMRYIAELERKV 220

Query: 208 QTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLK 267
           QTLQTEATTLSAQLAL QRDT+GL++EN+ELKLRLQ MEQQ HL+DALN+ LK+EV+RLK
Sbjct: 221 QTLQTEATTLSAQLALLQRDTSGLTTENSELKLRLQTMEQQVHLQDALNDTLKSEVQRLK 280

Query: 268 VATGE-TSGASDSFNLGGMPHMPYLASSFFPLHQQPGS-AGTHNMLQMPLYGHSSSNMSI 306
           VATG+  +G     N GGMPH        F  +Q   S    H + Q+ L+  +     +
Sbjct: 281 VATGQMANGGGMMMNFGGMPHQFGGNQQMFQNNQAMQSMLAAHQLQQLQLHPQAQQQQVL 339

BLAST of Cp4.1LG02g11360 vs. ExPASy Swiss-Prot
Match: Q04088 (Probable transcription factor PosF21 OS=Arabidopsis thaliana OX=3702 GN=POSF21 PE=2 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 9.3e-46
Identity = 127/267 (47.57%), Postives = 161/267 (60.30%), Query Frame = 0

Query: 37  HRRAHSELTFRFPDDI-MDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKL------ 96
           HRRAHSE+    PDD+  D        A   A   DE  +E+DL S Y+D+DK       
Sbjct: 58  HRRAHSEI-LTLPDDLSFDSDLGVVGNAADGASFSDE--TEEDLLSMYLDMDKFNSSATS 117

Query: 97  --------------------GPG-TSSADHTANAEPEKTPRPRHRYSNSVDGSTSTSVF- 156
                               G G TS+  +T N+  E+ PR RH++S S+DGS + +   
Sbjct: 118 SAQVGEPSGTAWKNETMMQTGTGSTSNPQNTVNSLGER-PRIRHQHSQSMDGSMNINEML 177

Query: 157 ------GDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKV 216
                    +DAKK+M   KLAEL  +DPKRAKRI ANRQSA+RSKERK RY+ ELERKV
Sbjct: 178 MSGNEDDSAIDAKKSMSATKLAELALIDPKRAKRIWANRQSAARSKERKTRYIFELERKV 237

Query: 217 QTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLK 269
           QTLQTEATTLSAQL L QRDT GL+ EN ELKLRLQ MEQQ HL+D LNEALK E++ LK
Sbjct: 238 QTLQTEATTLSAQLTLLQRDTNGLTVENNELKLRLQTMEQQVHLQDELNEALKEEIQHLK 297

BLAST of Cp4.1LG02g11360 vs. ExPASy Swiss-Prot
Match: Q8H1F0 (bZIP transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=BZIP29 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 6.7e-36
Identity = 97/170 (57.06%), Postives = 117/170 (68.82%), Query Frame = 0

Query: 115 SNSVDGSTSTSV---FGD----VLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSK 174
           +NSVDG++  +    F +      + KK M  DKLAE+   DPKR KRILANRQSA+RSK
Sbjct: 353 TNSVDGNSGAAFSIEFNNGEFTAAEMKKIMANDKLAEMAMSDPKRVKRILANRQSAARSK 412

Query: 175 ERKARYMQELERKVQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRD 234
           ERK RY+ ELE KVQTLQTEATTLSAQL L QRD  GL+++N ELK RLQAMEQQA LRD
Sbjct: 413 ERKMRYIVELEHKVQTLQTEATTLSAQLTLLQRDMMGLTNQNNELKFRLQAMEQQARLRD 472

Query: 235 ALNEALKNEVERLKVATGETS-GASDSFNLGGMPHMPYLASSFFPLHQQP 277
           ALNEAL  EV+RLK+A GE+S   S+   +  +    +   +   L QQP
Sbjct: 473 ALNEALNGEVQRLKLAIGESSQNESERSKMQSLNAEMFQQLNISQLRQQP 522

BLAST of Cp4.1LG02g11360 vs. NCBI nr
Match: XP_023525940.1 (transcription factor RF2b-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 675 bits (1741), Expect = 3.31e-244
Identity = 352/352 (100.00%), Postives = 352/352 (100.00%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP
Sbjct: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120
           FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG
Sbjct: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120

Query: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180
           STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK
Sbjct: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180

Query: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240
           VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL
Sbjct: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240

Query: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300
           KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH
Sbjct: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300

Query: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352
           PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF
Sbjct: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352

BLAST of Cp4.1LG02g11360 vs. NCBI nr
Match: XP_022949363.1 (transcription factor RF2b-like [Cucurbita moschata])

HSP 1 Score: 669 bits (1725), Expect = 9.09e-242
Identity = 349/352 (99.15%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           MQDPASNPDSSISNNASTPN HLPPPPPP SSTRPHHRRAHSELTFRFPDDIMDLSSSDP
Sbjct: 1   MQDPASNPDSSISNNASTPNTHLPPPPPPPSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120
           FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG
Sbjct: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120

Query: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180
           STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK
Sbjct: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180

Query: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240
           VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL
Sbjct: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240

Query: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300
           KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH
Sbjct: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300

Query: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352
           PLHHQLNSHQFS+TMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF
Sbjct: 301 PLHHQLNSHQFSETMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352

BLAST of Cp4.1LG02g11360 vs. NCBI nr
Match: KAG6607373.1 (bZIP transcription factor 18, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037042.1 Transcription factor RF2b, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 666 bits (1719), Expect = 7.19e-241
Identity = 350/352 (99.43%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           MQDPASNPDSSISNNASTPN HLPPPPPP SSTRPHHRRAHSELTFRFPDDIMDLSSSDP
Sbjct: 1   MQDPASNPDSSISNNASTPNTHLPPPPPP-SSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120
           FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG
Sbjct: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120

Query: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180
           STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK
Sbjct: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180

Query: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240
           VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL
Sbjct: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240

Query: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300
           KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH
Sbjct: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300

Query: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352
           PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF
Sbjct: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 351

BLAST of Cp4.1LG02g11360 vs. NCBI nr
Match: XP_022997725.1 (transcription factor RF2b-like [Cucurbita maxima])

HSP 1 Score: 662 bits (1707), Expect = 4.85e-239
Identity = 348/352 (98.86%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           MQDPASNPDSSISNNASTPN HLPPPPPP SSTRPHHRRAHSELTFRFPDDIMDLSSSDP
Sbjct: 1   MQDPASNPDSSISNNASTPNTHLPPPPPP-SSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120
           FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG
Sbjct: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120

Query: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180
           STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK
Sbjct: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180

Query: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240
           VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL
Sbjct: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240

Query: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300
           KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH
Sbjct: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300

Query: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352
           P HHQLNSHQFSD MQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF
Sbjct: 301 PPHHQLNSHQFSDNMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 351

BLAST of Cp4.1LG02g11360 vs. NCBI nr
Match: XP_022149560.1 (transcription factor RF2b [Momordica charantia])

HSP 1 Score: 427 bits (1099), Expect = 7.28e-146
Identity = 241/328 (73.48%), Postives = 269/328 (82.01%), Query Frame = 0

Query: 36  HHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKLGP--GT 95
           HHRRAHSE++FR P+D+MDLS SDPF  GS+  +L+EIGSEDDLFSTYIDV KLG   G 
Sbjct: 69  HHRRAHSEVSFRLPEDMMDLSGSDPFNGGSSTASLEEIGSEDDLFSTYIDVKKLGGNGGG 128

Query: 96  SSADHTAN--------AEPEKTPRPRHRYSNSVDGSTSTS-VFGDVLDAKKAMPPDKLAE 155
           + ADH AN        +E EKT +PRHR+S SVDG+TS+S +FG++++AKKAMPPDKLAE
Sbjct: 129 NYADHNANGGSEGAGGSEGEKTSKPRHRHSVSVDGTTSSSSMFGEIMEAKKAMPPDKLAE 188

Query: 156 LWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQTLQTEATTLSAQLALFQRDTTG 215
           LWT DPKRAKRILANRQSA+RSKERKARY+QELERKVQTLQTEATTLSAQL LFQRDTTG
Sbjct: 189 LWTSDPKRAKRILANRQSAARSKERKARYIQELERKVQTLQTEATTLSAQLTLFQRDTTG 248

Query: 216 LSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVATGETSGASDSFNLGGMPHMPY 275
           LS+ENTELKLRLQAMEQQA LRDALNEALK EVERL++ATGE    S+SFNLG M HM Y
Sbjct: 249 LSTENTELKLRLQAMEQQAQLRDALNEALKKEVERLRIATGEIMSPSESFNLG-MHHMAY 308

Query: 276 LASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIHPLHHQLNSHQFSDTMQSDPLGRLQ 335
             SSF  L  QPGSAG  NM QMP Y HS SNMS HPLH Q +SH  S+ +QSDP+GRLQ
Sbjct: 309 APSSFIQLPPQPGSAGPQNM-QMPPYSHSLSNMSSHPLH-QSDSHSLSEVLQSDPIGRLQ 368

Query: 336 GLDISSNGSPLVKSEVPSLSASESSATF 352
           GLDISS G  LVKSE PSLSASESS TF
Sbjct: 369 GLDISSKGPSLVKSEGPSLSASESSTTF 393

BLAST of Cp4.1LG02g11360 vs. ExPASy TrEMBL
Match: A0A6J1GBT8 (transcription factor RF2b-like OS=Cucurbita moschata OX=3662 GN=LOC111452738 PE=4 SV=1)

HSP 1 Score: 669 bits (1725), Expect = 4.40e-242
Identity = 349/352 (99.15%), Postives = 350/352 (99.43%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           MQDPASNPDSSISNNASTPN HLPPPPPP SSTRPHHRRAHSELTFRFPDDIMDLSSSDP
Sbjct: 1   MQDPASNPDSSISNNASTPNTHLPPPPPPPSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120
           FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG
Sbjct: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120

Query: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180
           STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK
Sbjct: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180

Query: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240
           VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL
Sbjct: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240

Query: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300
           KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH
Sbjct: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300

Query: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352
           PLHHQLNSHQFS+TMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF
Sbjct: 301 PLHHQLNSHQFSETMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352

BLAST of Cp4.1LG02g11360 vs. ExPASy TrEMBL
Match: A0A6J1KAR1 (transcription factor RF2b-like OS=Cucurbita maxima OX=3661 GN=LOC111492598 PE=4 SV=1)

HSP 1 Score: 662 bits (1707), Expect = 2.35e-239
Identity = 348/352 (98.86%), Postives = 348/352 (98.86%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           MQDPASNPDSSISNNASTPN HLPPPPPP SSTRPHHRRAHSELTFRFPDDIMDLSSSDP
Sbjct: 1   MQDPASNPDSSISNNASTPNTHLPPPPPP-SSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120
           FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG
Sbjct: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKTPRPRHRYSNSVDG 120

Query: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180
           STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK
Sbjct: 121 STSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERK 180

Query: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240
           VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL
Sbjct: 181 VQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERL 240

Query: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300
           KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH
Sbjct: 241 KVATGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIH 300

Query: 301 PLHHQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 352
           P HHQLNSHQFSD MQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF
Sbjct: 301 PPHHQLNSHQFSDNMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 351

BLAST of Cp4.1LG02g11360 vs. ExPASy TrEMBL
Match: A0A6J1D622 (transcription factor RF2b OS=Momordica charantia OX=3673 GN=LOC111017966 PE=4 SV=1)

HSP 1 Score: 427 bits (1099), Expect = 3.52e-146
Identity = 241/328 (73.48%), Postives = 269/328 (82.01%), Query Frame = 0

Query: 36  HHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKLGP--GT 95
           HHRRAHSE++FR P+D+MDLS SDPF  GS+  +L+EIGSEDDLFSTYIDV KLG   G 
Sbjct: 69  HHRRAHSEVSFRLPEDMMDLSGSDPFNGGSSTASLEEIGSEDDLFSTYIDVKKLGGNGGG 128

Query: 96  SSADHTAN--------AEPEKTPRPRHRYSNSVDGSTSTS-VFGDVLDAKKAMPPDKLAE 155
           + ADH AN        +E EKT +PRHR+S SVDG+TS+S +FG++++AKKAMPPDKLAE
Sbjct: 129 NYADHNANGGSEGAGGSEGEKTSKPRHRHSVSVDGTTSSSSMFGEIMEAKKAMPPDKLAE 188

Query: 156 LWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQTLQTEATTLSAQLALFQRDTTG 215
           LWT DPKRAKRILANRQSA+RSKERKARY+QELERKVQTLQTEATTLSAQL LFQRDTTG
Sbjct: 189 LWTSDPKRAKRILANRQSAARSKERKARYIQELERKVQTLQTEATTLSAQLTLFQRDTTG 248

Query: 216 LSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVATGETSGASDSFNLGGMPHMPY 275
           LS+ENTELKLRLQAMEQQA LRDALNEALK EVERL++ATGE    S+SFNLG M HM Y
Sbjct: 249 LSTENTELKLRLQAMEQQAQLRDALNEALKKEVERLRIATGEIMSPSESFNLG-MHHMAY 308

Query: 276 LASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIHPLHHQLNSHQFSDTMQSDPLGRLQ 335
             SSF  L  QPGSAG  NM QMP Y HS SNMS HPLH Q +SH  S+ +QSDP+GRLQ
Sbjct: 309 APSSFIQLPPQPGSAGPQNM-QMPPYSHSLSNMSSHPLH-QSDSHSLSEVLQSDPIGRLQ 368

Query: 336 GLDISSNGSPLVKSEVPSLSASESSATF 352
           GLDISS G  LVKSE PSLSASESS TF
Sbjct: 369 GLDISSKGPSLVKSEGPSLSASESSTTF 393

BLAST of Cp4.1LG02g11360 vs. ExPASy TrEMBL
Match: A0A6J1JCI4 (transcription factor RF2b-like OS=Cucurbita maxima OX=3661 GN=LOC111484488 PE=4 SV=1)

HSP 1 Score: 426 bits (1095), Expect = 1.16e-145
Identity = 256/390 (65.64%), Postives = 291/390 (74.62%), Query Frame = 0

Query: 1   MQDPA-SNPDSSISNNASTP-NIHLPPPP----PPLSSTRP------------------- 60
           MQDPA SNP  S ++N   P N  LPPPP    PP+++                      
Sbjct: 1   MQDPAPSNPIHSPNSNQIPPLNAALPPPPAHKPPPVANATASSSMFANANAGNGSFVPRA 60

Query: 61  --HHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKLGP-- 120
             HHRRAHSE++FR  +D+MD+S+SDPF  GS+  +L+EIGSEDDLFSTYIDV K G   
Sbjct: 61  GSHHRRAHSEVSFRLQEDMMDISASDPFNGGSSTASLEEIGSEDDLFSTYIDVKKFGGNG 120

Query: 121 GTSSADHTANA--------EPEKTPRPRHRYSNSVDGSTSTS-VFGDVLDAKKAMPPDKL 180
           G +  DH AN         E EKT +PRHR+S SVDG+TS+S +FG++++AKKAMPPDKL
Sbjct: 121 GGNFVDHDANGGSEGAGGNEGEKTSKPRHRHSVSVDGTTSSSSMFGEIMEAKKAMPPDKL 180

Query: 181 AELWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQTLQTEATTLSAQLALFQRDT 240
           AELW+ DPKRAKRILANRQSA+RSKERKARY+QELERKVQTLQTEATTLSAQL LFQRDT
Sbjct: 181 AELWSSDPKRAKRILANRQSAARSKERKARYIQELERKVQTLQTEATTLSAQLTLFQRDT 240

Query: 241 TGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVATGETSGASDSFNLGGMPHM 300
           TGLS+ENTELKLRLQAMEQQA +RDALNEALK EVERLK+ATGE    S+SFNLG M HM
Sbjct: 241 TGLSTENTELKLRLQAMEQQAQMRDALNEALKKEVERLKIATGEMMSPSESFNLG-MHHM 300

Query: 301 PYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIHPLHHQLNSHQFSDTMQSDPLGR 352
            Y  SSF  L QQPGSAG  N +QMP Y HS SNMS HPLH   +SH  S+ +QSDPLGR
Sbjct: 301 AYAPSSFIQLSQQPGSAGPQN-IQMPPYNHSPSNMSSHPLHPS-DSHSLSEVLQSDPLGR 360

BLAST of Cp4.1LG02g11360 vs. ExPASy TrEMBL
Match: A0A6J1FUC1 (transcription factor RF2b-like OS=Cucurbita moschata OX=3662 GN=LOC111448861 PE=4 SV=1)

HSP 1 Score: 424 bits (1089), Expect = 8.82e-145
Identity = 255/389 (65.55%), Postives = 292/389 (75.06%), Query Frame = 0

Query: 1   MQDPA-SNPDSSISNNASTPNIH--LPPP----PPPLSSTRP------------------ 60
           MQDPA SNP  S ++N   P +H  LPPP    PPP+++                     
Sbjct: 1   MQDPAPSNPIHSPNSNQIPP-LHAALPPPAAHKPPPVANATASSSMFANAGNGSFVPRAG 60

Query: 61  -HHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKLGP--G 120
            HHRRAHSE++FR  +D+MD+S+SDPF  GS+  +L+EIGSEDDLFSTYIDV K G   G
Sbjct: 61  SHHRRAHSEVSFRLQEDMMDISASDPFNGGSSTASLEEIGSEDDLFSTYIDVKKFGGNGG 120

Query: 121 TSSADHTANA--------EPEKTPRPRHRYSNSVDGSTSTS-VFGDVLDAKKAMPPDKLA 180
            +  DH AN         E EKT +PRHR+S SVDG+TS+S +FG++++AKKAMPPDKLA
Sbjct: 121 GNFVDHDANGGSEGAGGNEEEKTSKPRHRHSVSVDGTTSSSSMFGEIMEAKKAMPPDKLA 180

Query: 181 ELWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQTLQTEATTLSAQLALFQRDTT 240
           ELW+ DPKRAKRILANRQSA+RSKERKARY+QELERKVQTLQTEATTLSAQL LFQRDTT
Sbjct: 181 ELWSSDPKRAKRILANRQSAARSKERKARYIQELERKVQTLQTEATTLSAQLTLFQRDTT 240

Query: 241 GLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVATGETSGASDSFNLGGMPHMP 300
           GLS+ENTELKLRLQAMEQQA +RDALNEALK EVERLK+ATGE    S+SFNLG M HM 
Sbjct: 241 GLSTENTELKLRLQAMEQQAQMRDALNEALKKEVERLKIATGEMMSPSESFNLG-MHHMA 300

Query: 301 YLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIHPLHHQLNSHQFSDTMQSDPLGRL 352
           Y  SSF  L QQPGSAG  N +QMP Y HS SN+S HPLH   +SH  S+ +QSDPLGRL
Sbjct: 301 YAPSSFIQLSQQPGSAGPQN-IQMPPYNHSPSNVSSHPLHPS-DSHSLSEVLQSDPLGRL 360

BLAST of Cp4.1LG02g11360 vs. TAIR 10
Match: AT2G40620.1 (Basic-leucine zipper (bZIP) transcription factor family protein )

HSP 1 Score: 295.4 bits (755), Expect = 5.9e-80
Identity = 206/389 (52.96%), Postives = 247/389 (63.50%), Query Frame = 0

Query: 1   MQDPASNPDSSISNNASTPNIHLPPPPPPLSSTRPHHRRAHSELTFRFPDDIMDLSSSDP 60
           M+DP SNP  + SN +  P +   P P P+    P+HRRAHSE+ FR P+   DL  S+P
Sbjct: 1   MEDP-SNPQPNQSNLSQCPPLATAPTPAPVRG--PYHRRAHSEVQFRLPE---DLDLSEP 60

Query: 61  FTAGSAALALDEIGSEDDLFSTYIDVDKLGPGTSSADHTANAEPEKT------------- 120
           F         DE+GSEDDLF +Y+D++KLG G+ SA  +A     ++             
Sbjct: 61  FG------GFDELGSEDDLFCSYMDIEKLGSGSGSASDSAGPSAPRSDNPFSAENGGAEA 120

Query: 121 --PRPRHRYSNSVDGSTSTSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASR 180
              RPRHR+S SVDGS++     + ++AKKAM PDKLAELW +DPKRAKRI+ANRQSA+R
Sbjct: 121 GNSRPRHRHSLSVDGSSTL----ESIEAKKAMAPDKLAELWVVDPKRAKRIIANRQSAAR 180

Query: 181 SKERKARYMQELERKVQTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHL 240
           SKERKARY+ ELERKVQTLQTEATTLSAQL+LFQRDTTGLSSENTELKLRLQ MEQQA L
Sbjct: 181 SKERKARYILELERKVQTLQTEATTLSAQLSLFQRDTTGLSSENTELKLRLQVMEQQAKL 240

Query: 241 RDALNEALKNEVERLKVATGETSGASDSFNLGGMPHMPYL---ASSFFPLHQQPGSAGTH 300
           RDALNE LK EVERLK ATGE S A D++NL GM HM Y      SFF  H Q       
Sbjct: 241 RDALNEQLKKEVERLKFATGEVSPA-DAYNL-GMAHMQYQQQPQQSFFQHHHQQ-QTDAQ 300

Query: 301 NMLQM--------PLYGHSSSNMSIHPLHHQL----------NSHQFSDTMQSDPLGRLQ 352
           N+ QM        P    + S+ +  P  HQL           SH +S+ M  D LGRLQ
Sbjct: 301 NLQQMTHQFHLFQPNNNQNQSSRTNPPTAHQLMHHATSNAPAQSHSYSEAMHEDHLGRLQ 360

BLAST of Cp4.1LG02g11360 vs. TAIR 10
Match: AT1G06850.1 (basic leucine-zipper 52 )

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-76
Identity = 188/349 (53.87%), Postives = 236/349 (67.62%), Query Frame = 0

Query: 19  PNIHLPPPPPPLSSTRP-----HHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEI 78
           P   + P   P+ +  P      HRR+ S       DD+      DP ++ +A  + D++
Sbjct: 12  PGATIIPSSDPIPNADPIPSSSFHRRSRS-------DDMSMFMFMDPLSS-AAPPSSDDL 71

Query: 79  GSEDDLFSTYIDVDKL----------GPGTSSADHTANAEPEKTPRPRHRYSNSVDGSTS 138
            S+DDLFS++IDVD L             ++S    AN  P  + RPRHR+SNSVD   +
Sbjct: 72  PSDDDLFSSFIDVDSLTSNPNPFQNPSLSSNSVSGAANPPPPPSSRPRHRHSNSVDAGCA 131

Query: 139 TSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQT 198
               GD++DAKKAMPP+KL+ELW +DPKRAKRILANRQSA+RSKERKARY+QELERKVQ+
Sbjct: 132 MYA-GDIMDAKKAMPPEKLSELWNIDPKRAKRILANRQSAARSKERKARYIQELERKVQS 191

Query: 199 LQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVA 258
           LQTEATTLSAQL L+QRDT GL++ENTELKLRLQAMEQQA LR+ALNEAL+ EVER+K+ 
Sbjct: 192 LQTEATTLSAQLTLYQRDTNGLANENTELKLRLQAMEQQAQLRNALNEALRKEVERMKME 251

Query: 259 TGETSGASDSFNLGGMPHMPYLASSFFPLHQQPGSAGTHNMLQMPLYGHSSSNMSIHPLH 318
           TGE SG SDSF++ GM  + Y +S+F  +    GS   H+M QM    HSS N    P+ 
Sbjct: 252 TGEISGNSDSFDM-GMQQIQYSSSTFMAIPPYHGSMNLHDM-QM----HSSFN----PM- 311

Query: 319 HQLNSHQFSDTMQSDPLGRLQGLDISSNGSPLVKSEVPSLSASESSATF 353
              NS   SD +Q+   GR+QGL+ISSN S LVKSE PSLSASESS+ +
Sbjct: 312 EMSNSQSVSDFLQN---GRMQGLEISSNSSSLVKSEGPSLSASESSSAY 337

BLAST of Cp4.1LG02g11360 vs. TAIR 10
Match: AT1G06850.2 (basic leucine-zipper 52 )

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 139/247 (56.28%), Postives = 173/247 (70.04%), Query Frame = 0

Query: 19  PNIHLPPPPPPLSSTRP-----HHRRAHSELTFRFPDDIMDLSSSDPFTAGSAALALDEI 78
           P   + P   P+ +  P      HRR+ S       DD+      DP ++ +A  + D++
Sbjct: 12  PGATIIPSSDPIPNADPIPSSSFHRRSRS-------DDMSMFMFMDPLSS-AAPPSSDDL 71

Query: 79  GSEDDLFSTYIDVDKL----------GPGTSSADHTANAEPEKTPRPRHRYSNSVDGSTS 138
            S+DDLFS++IDVD L             ++S    AN  P  + RPRHR+SNSVD   +
Sbjct: 72  PSDDDLFSSFIDVDSLTSNPNPFQNPSLSSNSVSGAANPPPPPSSRPRHRHSNSVDAGCA 131

Query: 139 TSVFGDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKVQT 198
               GD++DAKKAMPP+KL+ELW +DPKRAKRILANRQSA+RSKERKARY+QELERKVQ+
Sbjct: 132 MYA-GDIMDAKKAMPPEKLSELWNIDPKRAKRILANRQSAARSKERKARYIQELERKVQS 191

Query: 199 LQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLKVA 251
           LQTEATTLSAQL L+QRDT GL++ENTELKLRLQAMEQQA LR+ALNEAL+ EVER+K+ 
Sbjct: 192 LQTEATTLSAQLTLYQRDTNGLANENTELKLRLQAMEQQAQLRNALNEALRKEVERMKME 249

BLAST of Cp4.1LG02g11360 vs. TAIR 10
Match: AT2G31370.3 (Basic-leucine zipper (bZIP) transcription factor family protein )

HSP 1 Score: 185.7 bits (470), Expect = 6.6e-47
Identity = 127/267 (47.57%), Postives = 161/267 (60.30%), Query Frame = 0

Query: 37  HRRAHSELTFRFPDDI-MDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKL------ 96
           HRRAHSE+    PDD+  D        A   A   DE  +E+DL S Y+D+DK       
Sbjct: 58  HRRAHSEI-LTLPDDLSFDSDLGVVGNAADGASFSDE--TEEDLLSMYLDMDKFNSSATS 117

Query: 97  --------------------GPG-TSSADHTANAEPEKTPRPRHRYSNSVDGSTSTSVF- 156
                               G G TS+  +T N+  E+ PR RH++S S+DGS + +   
Sbjct: 118 SAQVGEPSGTAWKNETMMQTGTGSTSNPQNTVNSLGER-PRIRHQHSQSMDGSMNINEML 177

Query: 157 ------GDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKV 216
                    +DAKK+M   KLAEL  +DPKRAKRI ANRQSA+RSKERK RY+ ELERKV
Sbjct: 178 MSGNEDDSAIDAKKSMSATKLAELALIDPKRAKRIWANRQSAARSKERKTRYIFELERKV 237

Query: 217 QTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLK 269
           QTLQTEATTLSAQL L QRDT GL+ EN ELKLRLQ MEQQ HL+D LNEALK E++ LK
Sbjct: 238 QTLQTEATTLSAQLTLLQRDTNGLTVENNELKLRLQTMEQQVHLQDELNEALKEEIQHLK 297

BLAST of Cp4.1LG02g11360 vs. TAIR 10
Match: AT2G31370.2 (Basic-leucine zipper (bZIP) transcription factor family protein )

HSP 1 Score: 185.7 bits (470), Expect = 6.6e-47
Identity = 127/267 (47.57%), Postives = 161/267 (60.30%), Query Frame = 0

Query: 37  HRRAHSELTFRFPDDI-MDLSSSDPFTAGSAALALDEIGSEDDLFSTYIDVDKL------ 96
           HRRAHSE+    PDD+  D        A   A   DE  +E+DL S Y+D+DK       
Sbjct: 58  HRRAHSEI-LTLPDDLSFDSDLGVVGNAADGASFSDE--TEEDLLSMYLDMDKFNSSATS 117

Query: 97  --------------------GPG-TSSADHTANAEPEKTPRPRHRYSNSVDGSTSTSVF- 156
                               G G TS+  +T N+  E+ PR RH++S S+DGS + +   
Sbjct: 118 SAQVGEPSGTAWKNETMMQTGTGSTSNPQNTVNSLGER-PRIRHQHSQSMDGSMNINEML 177

Query: 157 ------GDVLDAKKAMPPDKLAELWTLDPKRAKRILANRQSASRSKERKARYMQELERKV 216
                    +DAKK+M   KLAEL  +DPKRAKRI ANRQSA+RSKERK RY+ ELERKV
Sbjct: 178 MSGNEDDSAIDAKKSMSATKLAELALIDPKRAKRIWANRQSAARSKERKTRYIFELERKV 237

Query: 217 QTLQTEATTLSAQLALFQRDTTGLSSENTELKLRLQAMEQQAHLRDALNEALKNEVERLK 269
           QTLQTEATTLSAQL L QRDT GL+ EN ELKLRLQ MEQQ HL+D LNEALK E++ LK
Sbjct: 238 QTLQTEATTLSAQLTLLQRDTNGLTVENNELKLRLQTMEQQVHLQDELNEALKEEIQHLK 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6S4P41.5e-8055.29Transcription factor RF2b OS=Oryza sativa subsp. japonica OX=39947 GN=RF2b PE=1 ... [more]
O228738.3e-7952.96bZIP transcription factor 18 OS=Arabidopsis thaliana OX=3702 GN=BZIP18 PE=1 SV=1[more]
Q69IL47.4e-5146.41Transcription factor RF2a OS=Oryza sativa subsp. japonica OX=39947 GN=RF2a PE=1 ... [more]
Q040889.3e-4647.57Probable transcription factor PosF21 OS=Arabidopsis thaliana OX=3702 GN=POSF21 P... [more]
Q8H1F06.7e-3657.06bZIP transcription factor 29 OS=Arabidopsis thaliana OX=3702 GN=BZIP29 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023525940.13.31e-244100.00transcription factor RF2b-like [Cucurbita pepo subsp. pepo][more]
XP_022949363.19.09e-24299.15transcription factor RF2b-like [Cucurbita moschata][more]
KAG6607373.17.19e-24199.43bZIP transcription factor 18, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022997725.14.85e-23998.86transcription factor RF2b-like [Cucurbita maxima][more]
XP_022149560.17.28e-14673.48transcription factor RF2b [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1GBT84.40e-24299.15transcription factor RF2b-like OS=Cucurbita moschata OX=3662 GN=LOC111452738 PE=... [more]
A0A6J1KAR12.35e-23998.86transcription factor RF2b-like OS=Cucurbita maxima OX=3661 GN=LOC111492598 PE=4 ... [more]
A0A6J1D6223.52e-14673.48transcription factor RF2b OS=Momordica charantia OX=3673 GN=LOC111017966 PE=4 SV... [more]
A0A6J1JCI41.16e-14565.64transcription factor RF2b-like OS=Cucurbita maxima OX=3661 GN=LOC111484488 PE=4 ... [more]
A0A6J1FUC18.82e-14565.55transcription factor RF2b-like OS=Cucurbita moschata OX=3662 GN=LOC111448861 PE=... [more]
Match NameE-valueIdentityDescription
AT2G40620.15.9e-8052.96Basic-leucine zipper (bZIP) transcription factor family protein [more]
AT1G06850.11.0e-7653.87basic leucine-zipper 52 [more]
AT1G06850.21.5e-5956.28basic leucine-zipper 52 [more]
AT2G31370.36.6e-4747.57Basic-leucine zipper (bZIP) transcription factor family protein [more]
AT2G31370.26.6e-4747.57Basic-leucine zipper (bZIP) transcription factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 226..246
NoneNo IPR availableCOILSCoilCoilcoord: 167..194
NoneNo IPR availableGENE3D1.20.5.170coord: 150..202
e-value: 1.4E-9
score: 39.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..61
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 312..352
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..121
NoneNo IPR availablePANTHERPTHR13690:SF122ATBZIP TRANSCRIPTION FACTORcoord: 11..349
NoneNo IPR availablePANTHERPTHR13690TRANSCRIPTION FACTOR POSF21-RELATEDcoord: 11..349
NoneNo IPR availableSUPERFAMILY57959Leucine zipper domaincoord: 151..202
IPR004827Basic-leucine zipper domainSMARTSM00338brlzneucoord: 147..211
e-value: 4.3E-16
score: 69.4
IPR004827Basic-leucine zipper domainPFAMPF00170bZIP_1coord: 151..207
e-value: 1.3E-8
score: 34.8
IPR004827Basic-leucine zipper domainPROSITEPS50217BZIPcoord: 149..212
score: 10.978449
IPR044759RF2-like transcription factor, bZIP domainCDDcd14703bZIP_plant_RF2coord: 152..201
e-value: 1.02023E-22
score: 87.6304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g11360.1Cp4.1LG02g11360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity