Cp4.1LG02g11280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG02g11280 vs. ExPASy Swiss-Prot
Match: Q9FGI1 (E3 ubiquitin-protein ligase listerin OS=Arabidopsis thaliana OX=3702 GN=At5g58410 PE=3 SV=1)

HSP 1 Score: 1519.6 bits (3933), Expect = 0.0e+00
Identity = 894/1998 (44.74%), Postives = 1222/1998 (61.16%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGD ARSKARPSSSSLAASLLPS SAA A GFGG++GS R  ++L+ +D+ASF D+D E
Sbjct: 4    PKGDAARSKARPSSSSLAASLLPSGSAA-AVGFGGYVGSSRFQTSLSNEDSASFLDLDSE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHL+RLSRKDPTTK              +KALASLSE +KQK GK++  IIPQWT   
Sbjct: 64   VAQHLQRLSRKDPTTK--------------IKALASLSELVKQKQGKELLPIIPQWT--- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                  FEYKKL++DY+RDVR ATH+ MTN+V  AGR+IAPHLKS+MGPWWFSQFD  SE
Sbjct: 124  ------FEYKKLILDYSRDVRRATHDVMTNVVTGAGRDIAPHLKSIMGPWWFSQFDLASE 183

Query: 217  VSQSAMQSLQ-------------AAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSD 276
            VSQ+A  S Q             AAFPAQEKR+ AL LC+ EIF YLEENLKLTP  LSD
Sbjct: 184  VSQAAKSSFQVGSSFGNSVFLVEAAFPAQEKRLHALNLCSAEIFAYLEENLKLTPQNLSD 243

Query: 277  KVVAKDELEEMHQQLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKG--SGE 336
            K +A DELEEM+QQ+    LV  L    +    E +               TG    + E
Sbjct: 244  KSLASDELEEMYQQMISSSLV-GLATLLDILLREPD--------------NTGSANINSE 303

Query: 337  TKHASKSRETAISFAEKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSM 396
            +K ASK+R  A S AEK+F+ HK F++ L+S+S  +RSAT+S+L S +KN+P  F E  +
Sbjct: 304  SKLASKARAVATSSAEKMFSSHKCFLNFLKSESPSIRSATYSLLSSFIKNVPEVFGEGDV 363

Query: 397  KTISGSILGAFQEKDPSCHSSMWDAVLLFSKRLPNCWTYVNVQKTFHLVLW--------- 456
            ++++ ++LG F+E +P+CHSSMW+AVLLFSK+ P  W Y+NV K+    LW         
Sbjct: 364  RSLAPALLGVFRENNPTCHSSMWEAVLLFSKKFPQSWVYLNVHKSVLNHLWQFLRNGCYG 423

Query: 457  ---------------------ESRNFSSTFSR---------------------------I 516
                                 ES  F   F +                           +
Sbjct: 424  SPQVSYPALILFLEVMPAQSVESDKFFVNFFKNLLAGRSMCESSSTDQLSLLRATTECFL 483

Query: 517  YGLEGT-------HSIRQMQ--------------------KGLRPLNNKRTAETPSTKYP 576
            +GL          +SI  +Q                    KG  P N +++AE       
Sbjct: 484  WGLRNASRYCDVPNSIHDLQVDLIDKVLVKILWADFTELSKGSIPPNQRKSAENLGMGNS 543

Query: 577  MSYLQDLRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGF 636
            +SYLQ+L +CI+EILSGI L+E +LLS F    Q++ ++M Q  D  E+ +G++ ++I F
Sbjct: 544  VSYLQELGRCILEILSGINLLEQNLLSFFCKAVQESFLNMLQQGD-LEIVAGSMRKMIDF 603

Query: 637  ILELEQLSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVREL 696
            +L LE+ S+ + + WPL   +GP L+ AFP IRS E  DGV+LLS ++SVFGPRK+V  L
Sbjct: 604  LLLLERYSVLEGESWPLHQFMGPLLSKAFPWIRSSELLDGVKLLSVSVSVFGPRKVVPVL 663

Query: 697  FICNNGMSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDER 756
               ++  +ST LS  + +++   + +++F E+F+PWC+ G +SS  AR DLL +L+DDE 
Sbjct: 664  I--DDIETSTLLSVEKEKNMSPEKLIKVFQEIFIPWCMDGYDSSTAARQDLLFSLLDDEC 723

Query: 757  FSHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKAN 816
            F+ QW+ VISY FN  H G         +LA +  LL++AR +IT   +    N    + 
Sbjct: 724  FTQQWSDVISYVFNQQHQGF-------NNLAAMKMLLEKARDEITKRSSGQELNQRIGSR 783

Query: 817  LGNWHHEHLESAAVAIAQSQAPFRSS---FTDSVLGGSVQSDCSSFVSRDTLIAIFEAVF 876
              +WHH  +ES A+++  S +   +S   F  SVLGGS Q    SFVSR +L+ I+  + 
Sbjct: 784  PEHWHHTLIESTAISLVHSSSATTTSAVQFLCSVLGGSTQDSSISFVSRSSLVLIYRGIL 843

Query: 877  QKLVSFLLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNL 936
            +KL+SF+  SPL    ++CSSLI +   +F +S SS DV+ +A F+ EV+D   F L +L
Sbjct: 844  EKLLSFIKQSPLCSVNDTCSSLIVE-AIAFDSS-SSVDVIVVAKFAAEVIDGSFFSLKSL 903

Query: 937  GEENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKI-YQF 996
             ++  LL ++L++I+ ID +  +    D+ L +  KE+R++R      V  +  K+  QF
Sbjct: 904  SQDATLLTTVLSSIFIIDLENRMTSLVDNTLSES-KEKRKDRNFVCDYVHAVCSKMDNQF 963

Query: 997  WKNCKTHNRKKYGSILIQFIRSAIFSDD---TEEIVSLCCQWMLEIKDQISQDHLEEQVI 1056
            WK+     RK   S L QF+RS +  +D     E+  LC   M E+ + +S D  +E+ I
Sbjct: 964  WKSINYDVRKSSASTLAQFLRSVVLLEDDLQPFELTLLCASRMTEVLEYLSLDQSDEENI 1023

Query: 1057 HGLSGLLPTXEIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIG 1116
             GL                          LL++ D WP W++P+  A  +      +   
Sbjct: 1024 CGL--------------------------LLLESDAWPIWVSPSSSASIDTHGMPVQLC- 1083

Query: 1117 FDIHKSGNHKLISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENS 1176
             ++ KS + + +S    L+ K+G+  F  G  ++                  F+ Q    
Sbjct: 1084 -ELRKSKSQRYVSFIDSLIMKLGIHRFIVGHKDHG-----------------FASQ---- 1143

Query: 1177 SPCLGEATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFN 1236
                           AWL  EILCTW+WPGG  + SFLP   ++ K   S   LL+S F+
Sbjct: 1144 ---------------AWLSVEILCTWEWPGGKVQTSFLPNLVSFCKDEPSSGGLLNSIFD 1203

Query: 1237 ILLDGALLCGSRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSL 1296
            ILL+GAL+      + + N+    +  + D+ EPFLRAL S L  + KE++WG ++A + 
Sbjct: 1204 ILLNGALVHVKDEEEGLGNMWVDFNNNIVDVVEPFLRALVSFLHILFKEDLWGEEEAMAA 1263

Query: 1297 FELLVRRLFIGEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENI--IQN 1356
            F+++  +LFIGE  + +CLRI+P I+S +I P+    T     GS      K+ +  ++ 
Sbjct: 1264 FKMITDKLFIGEETSKNCLRIIPYIMSIIISPL---RTKVKSGGS-----GKDTLLPLEV 1323

Query: 1357 TIEGWLQRVLLFPSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSL 1416
             +  WL+R L FP L  WQ+GED++DW  LVISCYP S        L+  R++STEE +L
Sbjct: 1324 LLRNWLERSLSFPPLVLWQSGEDIQDWFQLVISCYPVSDKAEEAKELQ--RHLSTEERTL 1383

Query: 1417 LLEIFRKQRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQ 1476
            LL++FRKQ+   G S+ V   P VQ+LL+ L++++V YC   FN++DW+F+   L   IQ
Sbjct: 1384 LLDLFRKQKQDPGASTVVTQLPAVQILLARLIMIAVSYCGNDFNEDDWDFVFSNLKRLIQ 1443

Query: 1477 PAVVVMEEIAESVNGII--VNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLF 1536
             AVVVMEE +E+VN  I  V+S      N+ LE L   V ISDP     ++NAL +FSL 
Sbjct: 1444 SAVVVMEETSENVNDFISGVSSMEKEKENDTLEGLGHIVFISDP-SINSAQNALSAFSLL 1503

Query: 1537 YGSFGLQGLKDMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIA 1596
                  + ++  + L     +  + V DRI+EG+LR+FFCTG++EAI  SY  +A +I+A
Sbjct: 1504 NALVNHKSVEGEDNLKSLADETWDPVKDRILEGVLRLFFCTGLTEAIAASYSPEAASIVA 1563

Query: 1597 SSRIELPHFWDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQY 1656
            S R++   FW+L+A  V  SS  AR+RAV+++EFWGLS+G +SSLY I+FS+ P+PSLQ 
Sbjct: 1564 SFRVDHLQFWELVAHLVVDSSPRARDRAVRAVEFWGLSRGSISSLYAIMFSSNPIPSLQL 1623

Query: 1657 AAYFMLSTEPISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLP 1716
            AAY +LSTEPIS  AI+ +  +  L+ ++  +Q S+     SE  +LL++E+  M+EKL 
Sbjct: 1624 AAYTVLSTEPISRLAIVAD-LNAPLNDESLNDQDSSNAGLPSEDKLLLRDEVSCMVEKLD 1683

Query: 1717 DDVFDMELMAQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHI 1776
             ++ D +L A ERV  FLAWSLLLS++ SLP  +  RERLVQYI+ +A+  ILD LFQHI
Sbjct: 1684 HELLDTDLTAPERVQTFLAWSLLLSNVNSLPSLTQGRERLVQYIEKTANPLILDSLFQHI 1743

Query: 1777 PVE---GMALQKKKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAI 1836
            P+E   G +L KKKD ++P+ LS  A+AA +AI TGS L  VE LWP+E  K+AS AGAI
Sbjct: 1744 PLELYMGQSL-KKKDGDIPSELSVVASAATRAIITGSSLSTVESLWPIETGKMASLAGAI 1803

Query: 1837 YGLMLRVLPAYVRGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSV 1896
            YGLMLRVLPAYVR WF ++RDRS SS+IE+FT+ WCSPSLI NELSQIKKA+F DE+FSV
Sbjct: 1804 YGLMLRVLPAYVREWFSEMRDRSASSLIEAFTRTWCSPSLIKNELSQIKKADFNDESFSV 1863

Query: 1897 VVSKSANEVIATYTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSF 1922
             +SK+ANEV+ATYTKDETGMDLVIRLP SYPL+ VDV+C +S+GISE KQRKWL+SM  F
Sbjct: 1864 SISKAANEVVATYTKDETGMDLVIRLPVSYPLKPVDVNCAKSIGISEAKQRKWLMSMQMF 1872

BLAST of Cp4.1LG02g11280 vs. ExPASy Swiss-Prot
Match: Q6A009 (E3 ubiquitin-protein ligase listerin OS=Mus musculus OX=10090 GN=Ltn1 PE=1 SV=3)

HSP 1 Score: 208.4 bits (529), Expect = 7.3e-52
Identity = 434/2002 (21.68%), Postives = 777/2002 (38.81%), Query Frame = 0

Query: 43   RSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSD--IDGEVAQH 102
            +   RPS+S  AA LL  +      GF GF  S+     +     A   D  +D +    
Sbjct: 10   KGNLRPSNSGRAAELLAKEQ-GTVPGFIGFGTSHSDLGYVPAVQGAEDIDSLVDSDFRMV 69

Query: 103  LKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQW-TIACLEF 162
            L++LS+KD TTK              LKA+        ++  + V  ++P W  I C   
Sbjct: 70   LRKLSKKDVTTK--------------LKAMQEFGIMCTERDTEAVKGVLPYWPRIFC--- 129

Query: 163  WKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQ 222
                   K+ +D++R VR AT +    L++   + +AP+LKS+MG W  +Q D+    + 
Sbjct: 130  -------KISLDHDRRVREATQQAFEKLILKVKKHLAPYLKSVMGYWLMAQCDTYPPAAL 189

Query: 223  SAMQSLQAAFPAQEKRVDALILCTTEIFIYLEEN-LKLTPDTLSD-KVVAKDELE-EMHQ 282
            +A  + +AAFP   K+ +A+  C  EI   L+++ LK TPDTLSD + V ++E E + H+
Sbjct: 190  AAKDAFEAAFP-PSKQPEAIAFCKEEITTVLQDHLLKETPDTLSDPQTVPEEEREAKFHR 249

Query: 283  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 342
             +   +L              K  + F    +    +   K                   
Sbjct: 250  VVTCSLLAL------------KRLLCFLPNNELDSLEEKFK------------------- 309

Query: 343  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 402
              L +++K++ +   S    VRSA F ++ +L +++P   KE++ K +S S+L +  + D
Sbjct: 310  -SLLSQNKFWKYGKHSVPQ-VRSAYFELVSALCQHVPQVMKEEAAK-VSPSVLLSIDDSD 369

Query: 403  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTFHLVLWESRNFSSTFSRIYGL--EGTHSIR 462
            P    ++W+AVL     + +CW +VN +K             S F ++  +  EG   + 
Sbjct: 370  PVVCPALWEAVLYTLTTIEDCWFHVNAKK-------------SVFPKLMAMIREGGRGLA 429

Query: 463  Q-MQKGLRPLNNKRTAETPSTKYPMSYLQDLRKCIVEILSGIYL-----VEHDLLSVFAT 522
              M   L P  +K        K     L   +  +  +++G+          +  +V + 
Sbjct: 430  AVMYPYLLPFISKLPQSITEPK-----LDFFKNFLTSLVTGLSTERTKSSSSECSAVISA 489

Query: 523  EFQKNCISMFQLTDNTEVASGTI-EQIIGFILELEQLSMDKDDIWPLALLVGPTLANAFP 582
             F+     M Q     E+    I EQ+I FI      ++ KD      L  GP   +   
Sbjct: 490  FFECLRFIMQQNLGEEEMVQMLINEQLIPFI-----DTVLKDS----GLHHGPMFDHLAD 549

Query: 583  IIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGMSSTHLSGVEAQDVEARQFMQIFN 642
             + S E+         A  V+    ++   +     +S      +   + + +  + + +
Sbjct: 550  TLSSWEAKADAERDPGA--VYNLENVLLSFW---GRLSEICTEKIRQPEADVKSVLCVSS 609

Query: 643  EVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWNSVISYSFNLDHTGVVLESINSESL 702
             V V   LQ   SS    L L        RF+      I  +   D   +  E  NSE  
Sbjct: 610  LVGV---LQRPRSS----LKLHRKKTAQVRFAIN----IPEAHKGDEKSMSSEGENSEGS 669

Query: 703  AMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHEHLESAAVAIAQSQAPFRSSFTDS 762
                     A+  ++N+ + ++ +  +K  L +   +  E +   + + ++     F  +
Sbjct: 670  D------GGAQSPLSNTSS-DLVSPLRKKPLEDLVCKLAEVSISFVNERKSEQHLQFLST 729

Query: 763  VLG--GSVQ------SDCSSFVSRDTLIAIFE-AVFQKLVSFLLHSPLSWARNSCSSLIP 822
            +L    SVQ      SD    V +   + I + A     V FL H  + W  +S      
Sbjct: 730  LLDSFSSVQVFNILLSDKQKNVVKAKPLEITKLAEKNPAVKFLYHKLIGWLNDS-----Q 789

Query: 823  KPGNSFPNSISSSDVVAMAHFSLEVLDRCV---FCLYNLGEENYLLPSILATIYAIDWDC 882
            K    F        +V + + +L   D  V     L +L +E+    S+L     I+  C
Sbjct: 790  KEDGGF--------LVDILYSALRCCDSGVERKEVLDDLTKEDLKWSSLL---QVIEKAC 849

Query: 883  SIEERQ-------------------DDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKN 942
            S  ++                     D L DK  E          +  +LR  + Q  KN
Sbjct: 850  SSSDKHALVTPWLKGSILGEKLVALADCLCDKDLEATTSESHSSEQWSLLRLALSQHVKN 909

Query: 943  CKTHNRKKYGSILIQFIRSAIFSDDTEEI------VSLCCQWMLEIKDQISQDHLEEQVI 1002
                     G I+++   +   + D  E       VS  C                  V+
Sbjct: 910  DYLIGEVYVGRIIVKLHETLSKTKDLSEAANSDSSVSFVC-----------------DVV 969

Query: 1003 HGL-----SGLL--PTXEI---KDQISQDHLEEQYMLDQLLIK----------------G 1062
            H        GLL  P+ ++     Q+     E  ++ D L+ K                 
Sbjct: 970  HSFFSSAGGGLLMPPSEDLLLTLFQLCAQSKERTHLPDFLICKLKNTLLSGVNLLVHQTA 1029

Query: 1063 DTW---PFWIAPNLMAPNELAASNTKNIGFDIHKSGNHKLISL------ASMLMSKIGLE 1122
             T+    F     L   +++ +S   N    +  S    L+         S+L   IG  
Sbjct: 1030 STYEQSTFLRLSVLWLKDQVQSSALDNTSLQVLLSAAGDLLGTLVESEDTSLLGVYIGSV 1089

Query: 1123 NFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLGEATNNEV---TCRAWLVAEI 1182
                 + E     L    + +  LE   S   E       E     +    C + L++++
Sbjct: 1090 MPSDSEWEKMRQALPVQWLHRPLLEGRLSLNYECFKTDFKEQDTKTLPNHLCTSSLLSKM 1149

Query: 1183 LCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCGSRAAQSIV--NI 1242
            +   +        + L    A +  S      LD+  + L   +  CG     +I   N+
Sbjct: 1150 ILVAQKKKLVLEDNVLEKIIAELLYSLQWCEELDNAPSFL---SGFCGILQKMNITYSNL 1209

Query: 1243 SPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIGEAVNIDCLR 1302
            S   +T           +L  LLF   ++N        +L+ L++ +L +  +++ D ++
Sbjct: 1210 SVLSET----------SSLLQLLFDRSRKN-------GTLWSLIIAKLILSRSISSDEVK 1269

Query: 1303 --------ILPLIVSFL-----IRPMCERNTVFDDSGSC---SVEGSKENIIQNTIEGWL 1362
                      PL    L     + P   +    + S  C    +  +KE++   +I G  
Sbjct: 1270 PYYKRKESFFPLTEGSLHTIQSLCPFLSKEEKKEFSAQCIPAFLGWTKEDLC--SINGAF 1329

Query: 1363 QRVLLFPSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFR 1422
              + +F S  Q ++ +D +    L+       +S    H                 +IF 
Sbjct: 1330 GHLAIFNSCLQTRSIDDKQ----LLHGILKIITSWRKQHE----------------DIFL 1389

Query: 1423 KQRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVM 1482
               N+S  S  V       M    L +    Y      D +W+F++C +++W++      
Sbjct: 1390 FSCNLSEASPEVLGLNIEIMRFLSLFLKHCAYP-LPLADSEWDFIMCSMLAWLE--TTSE 1449

Query: 1483 EEIAESVNGIIVNSSTSMNANEILEKLEQSV--KISDPIPFCISRNALLSFSLFYGSFGL 1542
             +   SV  + + +  S +    L     S+   I D +P     N +  +  F+ S G+
Sbjct: 1450 NQALYSVPLVQLFACVSFDLACDLCAFFDSITPDIVDNLPV----NLISEWKEFF-SKGI 1509

Query: 1543 QGLKDMETLNPQRLDKLNHVNDRIVEGILRMFFC---TGISEAIVCSYGDKATTIIASSR 1602
              L  +  L    + +   +++   +  +    C   T IS+  + S+   A  ++AS +
Sbjct: 1510 HSL--LLPLLVNAIGENKDLSETSFQNAMLKPMCETLTYISKDQLLSHKLPA-RLVASQK 1569

Query: 1603 IELPHFWDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAY 1662
              LP     + +++T                      P+     +LF  +PV   Q AAY
Sbjct: 1570 TNLPEHLQTLLNTLT----------------------PL-----LLFRARPV---QIAAY 1629

Query: 1663 FMLSTEPISYSAIIRENTSCYLD--YDTTTEQGSTQVDFSSEYNVLLKEEI-------VF 1722
             ML           ++N   Y D   +      +  +   S    LL+  +       + 
Sbjct: 1630 HMLCKLMPELPQHDQDNLRSYGDEEEEPALSPPAALMSLLSSQEELLENVLGCVPVGQIV 1689

Query: 1723 MIEKLPDDVFDMELMAQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILD 1782
             ++ L +D   +       +   L W L+L+   +   SS  R     Y++ + S   L+
Sbjct: 1690 TVKPLSEDFCYV-------LGYLLTWKLILTFFKA--ASSQLRALYSMYLRKTKS---LN 1749

Query: 1783 CLFQHIPVEGMALQKKKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYA 1842
             L  H+      L  +  T     +  ++       T    L + E      P  +   A
Sbjct: 1750 KLLYHL----FRLMPENPTYGETAIEVSSKDPKTFFTEEVQLSIRE--TATLPYHIPHLA 1766

Query: 1843 GAIYGLMLRVLPAYVRGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADEN 1902
             ++Y + L+ LPA VR W+ +  ++   ++++ FT  + S  L   E+S ++ +      
Sbjct: 1810 CSVYHMTLKDLPAMVRLWW-NSSEKRVFNIVDRFTSKYVSNVLSFQEISSVQTSTQLFNG 1766

Query: 1903 FSVVVSKSANEVIATYTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSM 1923
             +V    +  EV+ATYT ++  ++L+I+LPS+YPL  + V+  + +G++  + R W+L +
Sbjct: 1870 MTVKARATTREVMATYTIEDIVIELIIQLPSNYPLGSITVESGKRIGVAVQQWRNWMLQL 1766

BLAST of Cp4.1LG02g11280 vs. ExPASy Swiss-Prot
Match: Q555H8 (E3 ubiquitin-protein ligase listerin OS=Dictyostelium discoideum OX=44689 GN=rnf160 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.6e-51
Identity = 448/2071 (21.63%), Postives = 813/2071 (39.26%), Query Frame = 0

Query: 44   SKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGEVAQHLKR 103
            +K  PSSS  A +L+ S S+       GF+G     S LT      +  ++ E    LK+
Sbjct: 12   AKYNPSSSGEANTLMGSQSS-------GFIGFSAFSSNLTNATDPDYYQVEPEYKVLLKK 71

Query: 104  LSRKDPTTKGSLIGTNLLLDFQQLKALASL-SEFLK---QKSGKDVASIIPQWTIACLEF 163
            L +KD                 ++K L  L S+F K   + +  +++SI P      +  
Sbjct: 72   LQKKD--------------SISRIKGLEELNSKFQKINIEDAEFNISSISP-----LMNA 131

Query: 164  WKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQ 223
            W+ F YK+L+ D +R+VR    + + ++    G+ + PH+K L+GPW  +  D   +   
Sbjct: 132  WE-FMYKRLVNDDDRNVRDLASQCLGSIGSRIGKHLGPHVKQLLGPWIVAICDQNDQSIN 191

Query: 224  SAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSD-KVVAKDELEEMHQQL 283
            +A+ S Q  FP ++KR D       E   YL ENL+ TP T+ D K ++++ L+E +++ 
Sbjct: 192  NALLSFQNIFP-EKKRKDVFKFGHDEALTYLCENLQETPQTIGDSKSISQEILQERYERC 251

Query: 284  HYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFAEK 343
                L+  +EY   + +      +  S T          G+ E               EK
Sbjct: 252  ISNSLL-AIEYLINNTTATATDSSSSSSTTTTTTTTNNTGNTE-------------IYEK 311

Query: 344  LFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKDPS 403
            +F     F    QSKS+ +R   + VL +++K +   + E++ K  S  +LG F EKD S
Sbjct: 312  IF--ESQFFSFFQSKSSNIRKTAYRVLTTVIKKLA-GYVEKNFKDFSSKVLGLFSEKDSS 371

Query: 404  CHSSMWDAVLLFSKRL-PNCWTYVNVQKTFHLVLWE------SRNFSSTFSRIYGL---- 463
             H  MWDA++ F ++     W+ V+V+K     LW         +F  ++  I  L    
Sbjct: 372  THLYMWDAIISFLQQYGDKAWSNVDVRKHVLPRLWAFLRSGCYGSFELSYPSILPLLTFI 431

Query: 464  -----EGTHS-------------------IRQMQKGLRPLNNKRTAETPSTKYPMSYLQD 523
                  G+ S                      ++KGL  ++N R    P++    S L  
Sbjct: 432  PNSIVIGSPSPSPSPSPNTNIDIGFFKELFTNLEKGLEVVDNPRQYGNPNSVDRPSNL-- 491

Query: 524  LRKCIVEIL--------SGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQII 583
            L  C +E L             +   L+      F  N    F L  +       IE+++
Sbjct: 492  LLSCYLECLIYSSKKWGQQYQQINDYLIDTLLFNFYNN---HFNLEHSCFSEKLFIEKLL 551

Query: 584  GFILELEQLSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVR 643
               ++L  LS                 +N   I     S+  +   +    +     I  
Sbjct: 552  ESTIKLANLSN----------------SNLLKIQNLFNSNSFINFNNKENLLNNNEPITT 611

Query: 644  ELFICNNGMSSTHLSGVE--AQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALI 703
            +    NN  SS  +   E   Q +     +   N  F+  C   NNS+     +++    
Sbjct: 612  DNNNNNNNNSSPIIKKQEKIIQPISKETLISRLN-TFIIVCSNNNNSNNSNNNNVI---- 671

Query: 704  DDERFSHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTW 763
                       +I   FNL       +++NS    +  K+LD                  
Sbjct: 672  ---------QVIIKRLFNL-----ACDNLNSNQ-ELSIKILDFI---------------- 731

Query: 764  QKANLGNWHHEHLES----------AAVAIAQSQAPFRSSFTDSVLGGSVQSDCSSFVS- 823
                LGN+  + + S            +A+A +++   +   +  +GG + S     ++ 
Sbjct: 732  ----LGNYKIDQIISNGLDDIKKIITQLALALNESLLSTGGLNLGVGGDLVSVIVGLLAL 791

Query: 824  ---RDTLIAIFEAVFQ-KLVSFLLHSPLSWARNSCSSLIPKPGNSFPNSISSSD------ 883
                D +  I+  + Q  L S   ++  S  +N+ S+LI    NS  + I+S+       
Sbjct: 792  VPDEDEIKLIWSQILQIFLDSINNNNNNSKKKNNRSNLITNTTNSLVHLINSASNKINRN 851

Query: 884  -VVAMAHF-SLEVLDRCVFCLY----------NLGEENYLLPS----------------I 943
             +++   F S+++ D+    L           N  E + ++P+                I
Sbjct: 852  LLLSTTLFKSIKIQDKKQLQLIKSILFNNNNNNNNEFSPIIPNESVELLIKWVYNKLVDI 911

Query: 944  LATIYAI-----DWDCSIEERQDDMLDDKFKE----ERRERLLFGGRV--RVLRQKIYQF 1003
            + T+  +     D D   +   DD  DD            +LLF  R   + L   + ++
Sbjct: 912  INTMVQLNLVFNDNDKGGDNNNDDDSDDNDVSIDYLVELSKLLFSSRFKSKSLESLLIEW 971

Query: 1004 WK-NCKTHNR-------KKYGSILIQFIRSAIFSDDTEEIVSLCCQWM------LEIKDQ 1063
             K N + +NR        K  SI      +    ++ + I+SL  + +      +   D 
Sbjct: 972  CKFNIEINNRPSLSGFKSKCNSIDEYLFSTTSNIENDKFILSLLIESIDRNSSTIITNDN 1031

Query: 1064 ISQDHLEEQVIHGL-SGLLPTXEIKDQISQD-----HLEEQYMLDQLLI--KGDTWPFWI 1123
            ++ + L    I  + SG     E  D    D     +  +++  +QL+I  K      W 
Sbjct: 1032 LNLNFLSNCAIKLIKSGSYRHDENGDDDDDDDQLYSYFIKEFTSNQLVINSKDQIKISWF 1091

Query: 1124 APNLMAPNELAASNTKNIGFDIHKSGNHKLISLASMLMSKIGLENFFSGQVENSSPCLAS 1183
              N + P  L  +   N         N+   + ++  + K+ + NF              
Sbjct: 1092 RYNRITPFYLLDNYGSN-------KDNNDSTTFSTDKLLKVLIFNF-------------- 1151

Query: 1184 MLMSKIGLEKF--FSGQVENSSPCLGEATNNEVTCRAWLVAEILCTWKWPGGNARGSFLP 1243
             ++   GLEKF  +S +  N+S                 +  +L    +  G+ +    P
Sbjct: 1152 KVIESFGLEKFIKYSNKENNNS-----------------IELLLLNLLFVNGDNQLPPTP 1211

Query: 1244 LFCAYVKRSCSHESLLDSTFNILLDGALLCGSRAAQSIVNISPYPDTLLEDIQEPFLRAL 1303
                    + +   ++ +   IL            + I N S     LL    E   +  
Sbjct: 1212 TTTTTTTTTITTAVVVSTNEQIL------------EFIENNS---TNLLNQFIESLFKFY 1271

Query: 1304 ASLL-----FSMLKENIWGRDKASSLFELLVRRLFIGEAVNIDCLRILPL-IVSFLIRPM 1363
               +     F+ L  N+ G      ++ +L+  LFI    +  CL    + + S L+  +
Sbjct: 1272 GDEIFNCNWFNCLVSNVGGDGNNGGIYSVLL-ALFIDYITSDACLPTSSMKLKSALLNSI 1331

Query: 1364 CERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLFPSLSQWQAGEDMEDWLLLVISCY 1423
               N   DD+G+      + N++Q          +L P L Q ++ E  + W +  +S  
Sbjct: 1332 KIDN---DDNGNSIDSIRQLNLVQ----------ILLPIL-QKESIESYKQWSINQLS-- 1391

Query: 1424 PFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNISGRSSTVNH--APRVQMLLSE--- 1483
              SSS+         ++IS + S L   + ++Q+    +S             +L E   
Sbjct: 1392 QLSSSIP-----TNFKSISIQLSILSSIVIQRQQQQHQQSQKEQQQITQDDNEILKEKEW 1451

Query: 1484 LMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESVNG---IIVNSSTSMNAN 1543
            L ++++    +Q+N +    ++ Q    ++   + M+E+  S      I++   +++N+N
Sbjct: 1452 LTLLNLIQTIQQWNSKIKSAIIKQQEPELE--FLFMKEVELSCLNFILIVIQYISNINSN 1511

Query: 1544 EILEKL----EQSVKISDPIPFCISRN-ALLSFSLFYGSFGLQGLKDMETLNPQRLDKLN 1603
            +  + +     QS  +   +   +S N  LLSF+ F  ++  Q  +++      ++  + 
Sbjct: 1512 KSKQPIYLSNNQSQFVQSFLSIWLSNNFTLLSFNKFKSNYYYQQYRNLIEYQSLKIYNII 1571

Query: 1604 HVNDRIVEGILRMFFCTGISEAIVCSYG----DKATTIIASSR--------IELPHFWDL 1663
              ND I       F   GI +     +     +     +++SR         +  +  +L
Sbjct: 1572 ISNDNI------KFIIPGIGDQFQIDHSILILNNWLNRVSNSRSNNNNGNTSDSSYILEL 1631

Query: 1664 IASSVTKSSKDAR--------ERAVKSIEFWGLS------KGPVSSLYGILFSTKPVPSL 1723
             +++  K S D +        +  ++ +  +  S      KG   + Y +L S     + 
Sbjct: 1632 YSNTFMKMSNDIKYKISLTKLDNLIELLSTFNYSTSIYNNKGIKKTSYNLLESYFGNENT 1691

Query: 1724 QYAAYFMLSTEPISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEK 1783
            Q    F+L  E          N +     +   + G  +V F    N+  K  + F    
Sbjct: 1692 QLVKSFLLENE---------NNNN-----NNNNDDGEEKVSFILFSNIFEKILLDFDSTF 1751

Query: 1784 LP-DDVFDMELMAQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLF 1843
             P     D +L+    +   + W+LLL +   +    + R  L  ++ ++   ++   L 
Sbjct: 1752 KPMKKSKDNQLINSISMGQLMCWNLLLLNYSKI--DLNRRSILNGWLNDNNIQKVTLFLN 1811

Query: 1844 QHIPVEGMALQKKKDTELPAGLSEAATAANQAITTGSLLFLVEFLWP-VEPVKLASY--- 1903
            Q  P        K ++ L    +      N         + +EF +  ++  +L +Y   
Sbjct: 1812 QIFP-----FINKDESPLTTTTTTTDNNGNN--------YSIEFPFSIIDNDQLITYRMI 1861

Query: 1904 ----AGAIYGLMLRVLPAYVRGWFCDLRDRSK-SSVIESFTKAWCSPSLIANELSQIKK- 1921
                +   Y L +  LP+ VR W  D    SK +  I+S+   + SP +I NE+ ++   
Sbjct: 1872 QKLCSNCFYSL-VNYLPSMVRRWADDNSTNSKMNQQIQSYITRFISPIIIQNEIQRVNSY 1861

BLAST of Cp4.1LG02g11280 vs. ExPASy Swiss-Prot
Match: A0JM49 (E3 ubiquitin-protein ligase listerin OS=Xenopus tropicalis OX=8364 GN=ltn1 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 8.4e-48
Identity = 107/300 (35.67%), Postives = 175/300 (58.33%), Query Frame = 0

Query: 1624 LAWSLLLSHLWSLPPSSSARERLVQYIQNSAS-SRILDCLFQHIPVEGMALQKKKDTELP 1683
            L W L+L+   +   SS  R    QY++ + S +++L  LF+ +P          +  LP
Sbjct: 1409 LTWKLILTFFKA--ASSQLRALYSQYLRKTKSLNKLLYNLFKLMP---------HNPVLP 1468

Query: 1684 AGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCDL 1743
               S+  +   +   T  L   V+    V+  ++   A  +Y + L+ LPA VR W+   
Sbjct: 1469 GQASDMPSKEPKTFFTEELQLAVKGTATVQQ-EVPHLACCVYHMTLKDLPAMVRLWWNGC 1528

Query: 1744 RDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETG 1803
              R   +V++ FT  + S  L + E+S ++ +    +  +V    +  EVIATY+ D+  
Sbjct: 1529 EKRI-FNVVDRFTTKYVSSVLSSQEISSVQLSTQVFDGMTVKARSTTREVIATYSVDDIC 1588

Query: 1804 MDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNF 1863
            ++L+I+LP +YPL  + V+  R +G++  + R W+L + +++ +QNG++ E + +WK N 
Sbjct: 1589 IELIIQLPQNYPLGSITVESGRRVGVAVQQWRNWMLQLNTYLTHQNGSIMEGLALWKNNV 1648

Query: 1864 DKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSPF 1923
            DK FEGVE+C IC+SVIH  N+S+P+ AC+TCK KFHS CLYKWF++S+KSTCPLC+  F
Sbjct: 1649 DKRFEGVEDCMICFSVIHGSNYSLPKKACRTCKKKFHSECLYKWFTSSNKSTCPLCRETF 1695

HSP 2 Score: 82.4 bits (202), Expect = 6.1e-14
Identity = 49/127 (38.58%), Postives = 71/127 (55.91%), Query Frame = 0

Query: 138 KQKSGKDVASIIPQWTIACLEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAP 197
           K++  + V  ++P W            Y K+ +D +R VR AT +    L++   + +AP
Sbjct: 9   KEREMETVRGVLPYWPRI---------YCKISLDLDRRVREATQQAFEQLILKVKKYLAP 68

Query: 198 HLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAFPAQEKRVDALILCTTEIF-IYLEENLKL 257
           HLKSLMG W  +Q D+ S  + SA  + +AAFPA  K+ +AL  C  EI  + L+  LK 
Sbjct: 69  HLKSLMGFWLIAQCDTYSPAASSARVAFEAAFPA-HKQPEALAFCKEEIMNVLLDHLLKE 125

Query: 258 TPDTLSD 264
           TPDTLSD
Sbjct: 129 TPDTLSD 125

BLAST of Cp4.1LG02g11280 vs. ExPASy Swiss-Prot
Match: E1C231 (E3 ubiquitin-protein ligase listerin OS=Gallus gallus OX=9031 GN=LTN1 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 2.4e-47
Identity = 108/301 (35.88%), Postives = 177/301 (58.80%), Query Frame = 0

Query: 1624 LAWSLLLSHLWSLPPSSSARERLVQYIQNSAS-SRILDCLFQHIPVEGMALQKKKDTELP 1683
            L W L L+   +   SS  R    QY++ + S +++L  LF+ +P          +  + 
Sbjct: 1479 LTWKLTLTFFKA--ASSQLRVLYSQYLRRTKSLNKLLYHLFRLMP----------ENPVF 1538

Query: 1684 AGL-SEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCD 1743
            +GL SE      +   T  L   V+    +   ++   A ++Y + L+ LPA VR W+  
Sbjct: 1539 SGLTSEVPNKDTKTFFTEELHLDVKGTGALSS-QIPHLACSVYHITLKDLPAMVRLWWNS 1598

Query: 1744 LRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDET 1803
               R   +V++ FT  + S  L + E+S ++ +       +V    +A EVIATY+ D+ 
Sbjct: 1599 CEKR-VFNVVDKFTSKYVSSVLSSQEISSVQTSTQLFNGMTVKARSAAREVIATYSVDDI 1658

Query: 1804 GMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRN 1863
             ++L+I+LPS+YPL  + V+  + +G++  + R W+L + +++ +QNG++ E + +WK N
Sbjct: 1659 FIELIIQLPSNYPLGSITVESGKRVGVAVQQWRNWMLQLSTYLTHQNGSIMEGLSLWKNN 1718

Query: 1864 FDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSP 1923
             DK FEG+E+C IC+SVIH  N+S+P+ AC+TCK KFHSACLYKWF++S+KSTCPLC+  
Sbjct: 1719 VDKRFEGIEDCMICFSVIHGSNYSLPKKACRTCKKKFHSACLYKWFTSSNKSTCPLCRET 1765

HSP 2 Score: 91.7 bits (226), Expect = 1.0e-16
Identity = 80/266 (30.08%), Postives = 123/266 (46.24%), Query Frame = 0

Query: 43  RSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSD--IDGEVAQH 102
           +   RPSSS  AA LL  +      GF GF  S      +     A   D  +D +    
Sbjct: 10  KGNVRPSSSGRAAELLAKE-RGTVPGFIGFGTSQSDLGYVPAVQGAEEIDSLVDADFRMV 69

Query: 103 LKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIACLEFW 162
           L++LS++D  TK              LKA+       K++  + V  ++P W        
Sbjct: 70  LRKLSKRDIITK--------------LKAMQEFGTMCKEREAEVVKGVLPYWPRI----- 129

Query: 163 KVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQS 222
               Y K+ +D++R VR AT ++   L++   + +AP+LKS+MG W  +Q D+ S  + +
Sbjct: 130 ----YCKISLDHDRRVREATQQSFEQLILKVKKHLAPYLKSIMGYWLIAQCDTYSPAASA 189

Query: 223 AMQSLQAAFPAQEKRVDALILCTTEIFIYLEEN-LKLTPDTLSDKVVAKDELEEMH---- 282
           A ++ + AFP+  K+ +AL  C  EI   L+++ LK TPDTLSD     +E  E      
Sbjct: 190 AKEAFEKAFPS-SKQPEALAFCKDEILNVLQDHLLKETPDTLSDPQTVPEEEREAKFFRI 249

Query: 283 ---QQLHYQILVFPLEYKSEHYSEEK 299
                L  + L+  L  K  H  EEK
Sbjct: 250 LTCSLLALKKLLSMLPKKEMHSLEEK 250

BLAST of Cp4.1LG02g11280 vs. NCBI nr
Match: XP_023523677.1 (LOW QUALITY PROTEIN: E3 ubiquitin-protein ligase listerin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 3369 bits (8735), Expect = 0.0
Identity = 1747/1921 (90.94%), Postives = 1761/1921 (91.67%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSEFLKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEFLKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 336
            Q+    L+                ++     + +R+  TGKGSGETKHASKSRETAISFA
Sbjct: 244  QVISSSLLALAT-----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFA 303

Query: 337  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 396
            EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD
Sbjct: 304  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 363

Query: 397  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTFHLVLW------------------------ 456
            PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT     W                        
Sbjct: 364  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISYPALILFLDT 423

Query: 457  -------ESRNFSSTFSRIY-GLEGTHSIRQMQKGLRPLNNKRTAETPSTKYPMSYLQDL 516
                   E +   + F  ++ G    HS    ++   PLNNKRTAETPSTKYPMSYLQDL
Sbjct: 424  VPPSAVGEQKFLLNFFENLWVGRNPFHSS-NAERLAXPLNNKRTAETPSTKYPMSYLQDL 483

Query: 517  RKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQL 576
            RKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQL
Sbjct: 484  RKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQL 543

Query: 577  SMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGM 636
            SMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGM
Sbjct: 544  SMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGM 603

Query: 637  SSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWNS 696
            SSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWNS
Sbjct: 604  SSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWNS 663

Query: 697  VISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHE 756
            VISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHE
Sbjct: 664  VISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHE 723

Query: 757  HLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSFL 816
            HLESAAVAIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSFL
Sbjct: 724  HLESAAVAIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSFL 783

Query: 817  LHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYLL 876
            LHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYLL
Sbjct: 784  LHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYLL 843

Query: 877  PSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTHN 936
            PSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTHN
Sbjct: 844  PSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTHN 903

Query: 937  RKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSGLLPTXE 996
            RKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQ             
Sbjct: 904  RKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQ------------- 963

Query: 997  IKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHKL 1056
                         YMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHKL
Sbjct: 964  -------------YMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHKL 1023

Query: 1057 ISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLGEATNNE 1116
            ISLASMLMSKIGLE                         KFFSGQVENSSPCLGEATNNE
Sbjct: 1024 ISLASMLMSKIGLE-------------------------KFFSGQVENSSPCLGEATNNE 1083

Query: 1117 VTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCGS 1176
            VTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCGS
Sbjct: 1084 VTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCGS 1143

Query: 1177 RAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIG 1236
            RAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIG
Sbjct: 1144 RAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIG 1203

Query: 1237 EAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLFP 1296
            EAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLFP
Sbjct: 1204 EAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLFP 1263

Query: 1297 SLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNISG 1356
            SLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNISG
Sbjct: 1264 SLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNISG 1323

Query: 1357 RSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESV 1416
            RSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESV
Sbjct: 1324 RSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESV 1383

Query: 1417 NGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMETL 1476
            NGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMETL
Sbjct: 1384 NGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMETL 1443

Query: 1477 NPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIAS 1536
            NPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIAS
Sbjct: 1444 NPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIAS 1503

Query: 1537 SVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSA 1596
            SVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSA
Sbjct: 1504 SVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSA 1563

Query: 1597 IIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERVN 1656
            IIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERVN
Sbjct: 1564 IIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERVN 1623

Query: 1657 IFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTEL 1716
            IFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTEL
Sbjct: 1624 IFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTEL 1683

Query: 1717 PAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCD 1776
            PAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCD
Sbjct: 1684 PAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCD 1743

Query: 1777 LRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDET 1836
            LRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDET
Sbjct: 1744 LRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDET 1803

Query: 1837 GMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRN 1896
            GMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRN
Sbjct: 1804 GMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRN 1837

Query: 1897 FDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSP 1922
            FDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSP
Sbjct: 1864 FDKEFEGVEECPICYSVIHTVNHSIPRLACKTCKHKFHSACLYKWFSTSHKSTCPLCQSP 1837

BLAST of Cp4.1LG02g11280 vs. NCBI nr
Match: KAG7037028.1 (E3 ubiquitin-protein ligase listerin [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 3325 bits (8621), Expect = 0.0
Identity = 1736/1982 (87.59%), Postives = 1757/1982 (88.65%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSEFLKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEFLKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 336
            Q+    L+                ++     + +R+  TGKGSGETKHASKSRETAISFA
Sbjct: 244  QVISSSLLALAT-----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFA 303

Query: 337  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 396
            EKLFTEHKYFVHLLQSKSTIVRSATFSV+KSLVKNIPHAFKEQ+MKTISGSILGAFQEKD
Sbjct: 304  EKLFTEHKYFVHLLQSKSTIVRSATFSVVKSLVKNIPHAFKEQNMKTISGSILGAFQEKD 363

Query: 397  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT------------------------------ 456
            PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT                              
Sbjct: 364  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISYPALILFLDT 423

Query: 457  --------------FHLVLWESRN--FSSTFSRI-----------YGLEGT--------- 516
                          F   LW  RN   SS   R+           +GL+           
Sbjct: 424  VPPSAVGEQKFLLNFFENLWVGRNPFHSSNAERLAFFQAFKECFLWGLQNASRFCSGDDL 483

Query: 517  ---------------------HSIRQMQK------GLRPLNNKRTAETPSTKYPMSYLQD 576
                                 H ++ ++       G  PLNNKRT ETPSTKYPMSYLQD
Sbjct: 484  AHFQVTLVDVVLVNLLWKDYLHDVQCLKNQDRALSGGAPLNNKRTGETPSTKYPMSYLQD 543

Query: 577  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 636
            LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ
Sbjct: 544  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 603

Query: 637  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 696
            LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGP  IVRELFICNNG
Sbjct: 604  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPHNIVRELFICNNG 663

Query: 697  MSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWN 756
            MSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNN SAGARLDLLL LIDDE FSHQWN
Sbjct: 664  MSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNCSAGARLDLLLTLIDDEHFSHQWN 723

Query: 757  SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 816
            SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH
Sbjct: 724  SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 783

Query: 817  EHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 876
            EHLESAAVAIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF
Sbjct: 784  EHLESAAVAIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 843

Query: 877  LLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL 936
            LLHSPLSWARNSCS LIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL
Sbjct: 844  LLHSPLSWARNSCSLLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL 903

Query: 937  LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH 996
            LPSILATIYAIDWDCSIEERQDD+LDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH
Sbjct: 904  LPSILATIYAIDWDCSIEERQDDLLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH 963

Query: 997  NRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSGLLPTX 1056
            NRKKYGSILIQFIRSAIFS+DTEEIVSLCCQWMLE+KDQIS+DHLEEQ            
Sbjct: 964  NRKKYGSILIQFIRSAIFSEDTEEIVSLCCQWMLEVKDQISEDHLEEQ------------ 1023

Query: 1057 EIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHK 1116
                          YMLDQLLIKGDTWPFWIAPN MAPNELAASNTKNIGFDIHKSGNHK
Sbjct: 1024 --------------YMLDQLLIKGDTWPFWIAPNFMAPNELAASNTKNIGFDIHKSGNHK 1083

Query: 1117 LISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLGEATNN 1176
            LISLASMLMSKIGLE                         KFFSGQVENSSPCLGEATNN
Sbjct: 1084 LISLASMLMSKIGLE-------------------------KFFSGQVENSSPCLGEATNN 1143

Query: 1177 EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG 1236
            EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG
Sbjct: 1144 EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG 1203

Query: 1237 SRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI 1296
            SRAAQSIVNI PYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI
Sbjct: 1204 SRAAQSIVNIWPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI 1263

Query: 1297 GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1356
            GEAVNIDCLRILPLIVSFLIRPMCERNT FDDSGSCSVEGSKENIIQNTIEGWLQRVLLF
Sbjct: 1264 GEAVNIDCLRILPLIVSFLIRPMCERNTEFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1323

Query: 1357 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS 1416
            PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDR ISTEESSLLLEIFRKQRNIS
Sbjct: 1324 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRKISTEESSLLLEIFRKQRNIS 1383

Query: 1417 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1476
            GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES
Sbjct: 1384 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1443

Query: 1477 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1536
            VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET
Sbjct: 1444 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1503

Query: 1537 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1596
            LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA
Sbjct: 1504 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1563

Query: 1597 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1656
            SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS
Sbjct: 1564 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1623

Query: 1657 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV 1716
            AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV
Sbjct: 1624 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV 1683

Query: 1717 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE 1776
            NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE
Sbjct: 1684 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE 1743

Query: 1777 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1836
            LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC
Sbjct: 1744 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1803

Query: 1837 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1896
            DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE
Sbjct: 1804 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1863

Query: 1897 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1922
            TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR
Sbjct: 1864 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1899

BLAST of Cp4.1LG02g11280 vs. NCBI nr
Match: XP_022998599.1 (E3 ubiquitin-protein ligase listerin isoform X1 [Cucurbita maxima])

HSP 1 Score: 3308 bits (8578), Expect = 0.0
Identity = 1732/1987 (87.17%), Postives = 1757/1987 (88.42%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSEFLKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEFLKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 336
            Q+    L+                ++     + +R+  TGKGSGETKHASKSRETAISFA
Sbjct: 244  QVISSSLLALAT-----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFA 303

Query: 337  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 396
            EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQ+MKTISGSILGAFQEKD
Sbjct: 304  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQNMKTISGSILGAFQEKD 363

Query: 397  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT------------------------------ 456
            PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT                              
Sbjct: 364  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISHPALILFLDT 423

Query: 457  --------------FHLVLWESRN--FSSTFSRI-----------YGLEGT--------- 516
                          F   LW  RN   SS   R+           +GL+           
Sbjct: 424  VPPSAVGEQKFLLNFFENLWVGRNPFHSSNAERLAFFQAFKECFLWGLQNASRFCSGDDL 483

Query: 517  ---------------------HSIRQMQK------GLRPLNNKRTAETPSTKYPMSYLQD 576
                                 H ++ ++       G  PLNNKRTAETPSTKYPMSYLQD
Sbjct: 484  AHFQVTLVDVILVNLLWKDYLHDVQCLKSQDRALSGGAPLNNKRTAETPSTKYPMSYLQD 543

Query: 577  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 636
            LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ
Sbjct: 544  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 603

Query: 637  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 696
            LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG
Sbjct: 604  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 663

Query: 697  MSSTHLSGVEAQDVEA-----RQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERF 756
            MSSTHLSGVEAQDVEA     RQFMQIFNEVFVPWCLQGNNSSAG+RLDLLLALIDDE F
Sbjct: 664  MSSTHLSGVEAQDVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGSRLDLLLALIDDEHF 723

Query: 757  SHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANL 816
            SHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANL
Sbjct: 724  SHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANL 783

Query: 817  GNWHHEHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQ 876
            GNWHHEHLESAAVAIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQ
Sbjct: 784  GNWHHEHLESAAVAIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQ 843

Query: 877  KLVSFLLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLG 936
            KLVSF+LHSPLSWARNSCS LIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLG
Sbjct: 844  KLVSFILHSPLSWARNSCSLLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLG 903

Query: 937  EENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWK 996
            EENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWK
Sbjct: 904  EENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWK 963

Query: 997  NCKTHNRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSG 1056
            NCKTHNRKKYGSILIQFIRSAIFS+DTEEIVSLCCQWMLEIKDQISQDHLEEQ       
Sbjct: 964  NCKTHNRKKYGSILIQFIRSAIFSEDTEEIVSLCCQWMLEIKDQISQDHLEEQ------- 1023

Query: 1057 LLPTXEIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHK 1116
                               YMLDQLLIKGDTWPFWIAPN MAPNELAASNTKNIGF IHK
Sbjct: 1024 -------------------YMLDQLLIKGDTWPFWIAPNFMAPNELAASNTKNIGFHIHK 1083

Query: 1117 SGNHKLISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLG 1176
            SGNHKLISLASMLMSKIGLE                         KFFSGQVE+SSPCLG
Sbjct: 1084 SGNHKLISLASMLMSKIGLE-------------------------KFFSGQVESSSPCLG 1143

Query: 1177 EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDG 1236
            EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSH SLL+STFNILLDG
Sbjct: 1144 EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHVSLLNSTFNILLDG 1203

Query: 1237 ALLCGSRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV 1296
            ALLCGSRAAQSIVNI PYPDTLL+DIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV
Sbjct: 1204 ALLCGSRAAQSIVNIWPYPDTLLDDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV 1263

Query: 1297 RRLFIGEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ 1356
            RRLFIGEAVNIDCLRILPLIV+FLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ
Sbjct: 1264 RRLFIGEAVNIDCLRILPLIVNFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ 1323

Query: 1357 RVLLFPSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRK 1416
            RVLLFPSLSQWQAGED EDWLLLVISCYPFSSSM GLHTLKPDRNISTEE SLLLEIFRK
Sbjct: 1324 RVLLFPSLSQWQAGEDAEDWLLLVISCYPFSSSMRGLHTLKPDRNISTEEGSLLLEIFRK 1383

Query: 1417 QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME 1476
            QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME
Sbjct: 1384 QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME 1443

Query: 1477 EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL 1536
            EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL
Sbjct: 1444 EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL 1503

Query: 1537 KDMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHF 1596
            K+METLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTII+SSRIELPHF
Sbjct: 1504 KNMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIISSSRIELPHF 1563

Query: 1597 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE 1656
            WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE
Sbjct: 1564 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE 1623

Query: 1657 PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM 1716
            PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM
Sbjct: 1624 PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM 1683

Query: 1717 AQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK 1776
            A ERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVE MALQK
Sbjct: 1684 AHERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVESMALQK 1743

Query: 1777 KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV 1836
            KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV
Sbjct: 1744 KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV 1803

Query: 1837 RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT 1896
            RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT
Sbjct: 1804 RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT 1863

Query: 1897 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1922
            YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI
Sbjct: 1864 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1904

BLAST of Cp4.1LG02g11280 vs. NCBI nr
Match: XP_022948558.1 (E3 ubiquitin-protein ligase listerin [Cucurbita moschata])

HSP 1 Score: 3308 bits (8577), Expect = 0.0
Identity = 1727/1982 (87.13%), Postives = 1753/1982 (88.45%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSEFLKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEFLKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 336
            Q+    L+                ++     + +R+  TGKGSGETKHASKSRETAISFA
Sbjct: 244  QVISSSLLALAT-----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFA 303

Query: 337  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 396
            EKLFTEHKYFVHLLQSKSTIVRSATFSV+KSLVKNIPHAFKEQ+MKTISGSILGAFQEKD
Sbjct: 304  EKLFTEHKYFVHLLQSKSTIVRSATFSVVKSLVKNIPHAFKEQNMKTISGSILGAFQEKD 363

Query: 397  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT------------------------------ 456
            PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT                              
Sbjct: 364  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISYPALILFLDT 423

Query: 457  --------------FHLVLWESRN--FSSTFSRI-----------YGLEGT--------- 516
                          F   LW  RN   SS   R+           +GL+           
Sbjct: 424  VPPSAVGEQKFLLNFFENLWVGRNPFHSSNAERLAFFQAFKECFLWGLQNASRFCSGDDL 483

Query: 517  ---------------------HSIRQMQK------GLRPLNNKRTAETPSTKYPMSYLQD 576
                                 H ++ ++       G  PLNNKRT ETPSTKYPMSYLQD
Sbjct: 484  AHFQVTLVDVILVNLLWKDYLHDVQCLKNQDRALSGGAPLNNKRTGETPSTKYPMSYLQD 543

Query: 577  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 636
            LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ
Sbjct: 544  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 603

Query: 637  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 696
            LSMDKDDIWPLA+LVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPR IVRELFICNNG
Sbjct: 604  LSMDKDDIWPLAILVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRNIVRELFICNNG 663

Query: 697  MSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWN 756
             SSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNN SAGARLDLLL LIDDE FSHQWN
Sbjct: 664  KSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNCSAGARLDLLLTLIDDEHFSHQWN 723

Query: 757  SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 816
            SVISYSF+LDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH
Sbjct: 724  SVISYSFDLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 783

Query: 817  EHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 876
            EHLESAAVAIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF
Sbjct: 784  EHLESAAVAIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 843

Query: 877  LLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL 936
            LLHSPLSWARNSCS LIPKPGNSFPNSISSSDVVA+AHFSLEVLDRCVFCLYNLGEENYL
Sbjct: 844  LLHSPLSWARNSCSLLIPKPGNSFPNSISSSDVVAIAHFSLEVLDRCVFCLYNLGEENYL 903

Query: 937  LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH 996
            LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH
Sbjct: 904  LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH 963

Query: 997  NRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSGLLPTX 1056
            +RKKYGSILIQFIRSAIFS+DTEEIVSLCCQWMLE+KDQISQDHLEEQ            
Sbjct: 964  DRKKYGSILIQFIRSAIFSEDTEEIVSLCCQWMLEVKDQISQDHLEEQ------------ 1023

Query: 1057 EIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHK 1116
                          YML QLLIKGDTWPFWIAPN MA NELA SNTKNIGFDIHKSGNHK
Sbjct: 1024 --------------YMLYQLLIKGDTWPFWIAPNFMASNELATSNTKNIGFDIHKSGNHK 1083

Query: 1117 LISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLGEATNN 1176
            LISLASMLMSKIGLE                         KFFSGQVENSSPCLGEATNN
Sbjct: 1084 LISLASMLMSKIGLE-------------------------KFFSGQVENSSPCLGEATNN 1143

Query: 1177 EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG 1236
            EVTCRAWLVAEILCTW WPGGNARGSFLPLFCAYVK+SCSHESLLDSTFNILLDGALLCG
Sbjct: 1144 EVTCRAWLVAEILCTWNWPGGNARGSFLPLFCAYVKKSCSHESLLDSTFNILLDGALLCG 1203

Query: 1237 SRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI 1296
            SRAAQSIVNI PYPD+LLEDIQEPFLRALASLLF MLKENIWGRDKASSLFELLVRRLFI
Sbjct: 1204 SRAAQSIVNIWPYPDSLLEDIQEPFLRALASLLFCMLKENIWGRDKASSLFELLVRRLFI 1263

Query: 1297 GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1356
            GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF
Sbjct: 1264 GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1323

Query: 1357 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS 1416
            PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS
Sbjct: 1324 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS 1383

Query: 1417 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1476
            GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES
Sbjct: 1384 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1443

Query: 1477 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1536
            VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET
Sbjct: 1444 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1503

Query: 1537 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1596
            LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA
Sbjct: 1504 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1563

Query: 1597 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1656
            SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS
Sbjct: 1564 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1623

Query: 1657 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV 1716
            AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMA ERV
Sbjct: 1624 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAHERV 1683

Query: 1717 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE 1776
            NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDC+FQHIPVEGMALQKKKDTE
Sbjct: 1684 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCIFQHIPVEGMALQKKKDTE 1743

Query: 1777 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1836
            LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC
Sbjct: 1744 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1803

Query: 1837 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1896
            DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE
Sbjct: 1804 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1863

Query: 1897 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1922
            TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR
Sbjct: 1864 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1899

BLAST of Cp4.1LG02g11280 vs. NCBI nr
Match: KAG6607353.1 (E3 ubiquitin-protein ligase listerin, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 3216 bits (8339), Expect = 0.0
Identity = 1694/1982 (85.47%), Postives = 1714/1982 (86.48%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSEFLKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEFLKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPD LSDKVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDILSDKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 336
            Q+    L+                ++     + +R+  TGKGSGETKHASKSRETAISFA
Sbjct: 244  QVISSSLLALAT-----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFA 303

Query: 337  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 396
            EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQ+MKTISGSILGAFQEKD
Sbjct: 304  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQNMKTISGSILGAFQEKD 363

Query: 397  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT------------------------------ 456
            PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT                              
Sbjct: 364  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISYPALILFLDT 423

Query: 457  --------------FHLVLWESRN--FSSTFSRI-----------YGLEGT--------- 516
                          F   LW  RN   SS   R+           +GL+           
Sbjct: 424  VPPSAVGEQKFLLNFFENLWVGRNPFHSSNAERLAFFQAFKECFLWGLQNASRFCSGDDL 483

Query: 517  ---------------------HSIRQMQK------GLRPLNNKRTAETPSTKYPMSYLQD 576
                                 H ++ ++       G  PLNNKRT ETPSTKYPMSYLQD
Sbjct: 484  AHFQVTLVDVILVNLLWKDYLHDVQCLKNQDRALSGGAPLNNKRTGETPSTKYPMSYLQD 543

Query: 577  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 636
            LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ
Sbjct: 544  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 603

Query: 637  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 696
            LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPR IVRELFICNNG
Sbjct: 604  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRNIVRELFICNNG 663

Query: 697  MSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWN 756
            +SSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNN SAGARLDLLL LIDDE FSHQWN
Sbjct: 664  ISSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNCSAGARLDLLLTLIDDEHFSHQWN 723

Query: 757  SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 816
            SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH
Sbjct: 724  SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 783

Query: 817  EHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 876
            EHLESAAVAIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF
Sbjct: 784  EHLESAAVAIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 843

Query: 877  LLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL 936
            LLHSPLSWARNSCS LIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL
Sbjct: 844  LLHSPLSWARNSCSLLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL 903

Query: 937  LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH 996
            LPSILATIYAIDWDCSIEERQDD+LDDKFKEERRE                         
Sbjct: 904  LPSILATIYAIDWDCSIEERQDDLLDDKFKEERRE------------------------- 963

Query: 997  NRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSGLLPTX 1056
                              S+DTEEIVSLCCQWMLE+KDQISQDHLEEQ            
Sbjct: 964  ------------------SEDTEEIVSLCCQWMLEVKDQISQDHLEEQ------------ 1023

Query: 1057 EIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHK 1116
                          YMLDQLLIKGDTWPFWIAPN MAPNELAASNTKNIGFDIHKSGNHK
Sbjct: 1024 --------------YMLDQLLIKGDTWPFWIAPNFMAPNELAASNTKNIGFDIHKSGNHK 1083

Query: 1117 LISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLGEATNN 1176
            LISLASMLMSKIGLE                         KFFSGQVENSSPCLGEATNN
Sbjct: 1084 LISLASMLMSKIGLE-------------------------KFFSGQVENSSPCLGEATNN 1143

Query: 1177 EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG 1236
            EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG
Sbjct: 1144 EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG 1203

Query: 1237 SRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI 1296
            SRAAQSIVNI PYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI
Sbjct: 1204 SRAAQSIVNIWPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI 1263

Query: 1297 GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1356
            GEAVNIDCLRILPLIVSFLIRPMCERNT FDDSGSCSVEGSKENIIQNTIEGWLQRVLLF
Sbjct: 1264 GEAVNIDCLRILPLIVSFLIRPMCERNTEFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1323

Query: 1357 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS 1416
            PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDR ISTEESSLLLEIFRKQRNIS
Sbjct: 1324 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRKISTEESSLLLEIFRKQRNIS 1383

Query: 1417 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1476
            GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES
Sbjct: 1384 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1443

Query: 1477 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1536
            VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET
Sbjct: 1444 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1503

Query: 1537 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1596
            LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA
Sbjct: 1504 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1563

Query: 1597 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1656
            SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS
Sbjct: 1564 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1623

Query: 1657 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV 1716
            AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV
Sbjct: 1624 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV 1683

Query: 1717 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE 1776
            NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE
Sbjct: 1684 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE 1743

Query: 1777 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1836
            LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC
Sbjct: 1744 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1803

Query: 1837 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1896
            DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE
Sbjct: 1804 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1856

Query: 1897 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1922
            TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR
Sbjct: 1864 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1856

BLAST of Cp4.1LG02g11280 vs. ExPASy TrEMBL
Match: A0A6J1KER5 (E3 ubiquitin-protein ligase listerin OS=Cucurbita maxima OX=3661 GN=LOC111493187 PE=3 SV=1)

HSP 1 Score: 3308 bits (8578), Expect = 0.0
Identity = 1732/1987 (87.17%), Postives = 1757/1987 (88.42%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSEFLKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEFLKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 336
            Q+    L+                ++     + +R+  TGKGSGETKHASKSRETAISFA
Sbjct: 244  QVISSSLLALAT-----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFA 303

Query: 337  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 396
            EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQ+MKTISGSILGAFQEKD
Sbjct: 304  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQNMKTISGSILGAFQEKD 363

Query: 397  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT------------------------------ 456
            PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT                              
Sbjct: 364  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISHPALILFLDT 423

Query: 457  --------------FHLVLWESRN--FSSTFSRI-----------YGLEGT--------- 516
                          F   LW  RN   SS   R+           +GL+           
Sbjct: 424  VPPSAVGEQKFLLNFFENLWVGRNPFHSSNAERLAFFQAFKECFLWGLQNASRFCSGDDL 483

Query: 517  ---------------------HSIRQMQK------GLRPLNNKRTAETPSTKYPMSYLQD 576
                                 H ++ ++       G  PLNNKRTAETPSTKYPMSYLQD
Sbjct: 484  AHFQVTLVDVILVNLLWKDYLHDVQCLKSQDRALSGGAPLNNKRTAETPSTKYPMSYLQD 543

Query: 577  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 636
            LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ
Sbjct: 544  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 603

Query: 637  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 696
            LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG
Sbjct: 604  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 663

Query: 697  MSSTHLSGVEAQDVEA-----RQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERF 756
            MSSTHLSGVEAQDVEA     RQFMQIFNEVFVPWCLQGNNSSAG+RLDLLLALIDDE F
Sbjct: 664  MSSTHLSGVEAQDVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGSRLDLLLALIDDEHF 723

Query: 757  SHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANL 816
            SHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANL
Sbjct: 724  SHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANL 783

Query: 817  GNWHHEHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQ 876
            GNWHHEHLESAAVAIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQ
Sbjct: 784  GNWHHEHLESAAVAIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQ 843

Query: 877  KLVSFLLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLG 936
            KLVSF+LHSPLSWARNSCS LIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLG
Sbjct: 844  KLVSFILHSPLSWARNSCSLLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLG 903

Query: 937  EENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWK 996
            EENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWK
Sbjct: 904  EENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWK 963

Query: 997  NCKTHNRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSG 1056
            NCKTHNRKKYGSILIQFIRSAIFS+DTEEIVSLCCQWMLEIKDQISQDHLEEQ       
Sbjct: 964  NCKTHNRKKYGSILIQFIRSAIFSEDTEEIVSLCCQWMLEIKDQISQDHLEEQ------- 1023

Query: 1057 LLPTXEIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHK 1116
                               YMLDQLLIKGDTWPFWIAPN MAPNELAASNTKNIGF IHK
Sbjct: 1024 -------------------YMLDQLLIKGDTWPFWIAPNFMAPNELAASNTKNIGFHIHK 1083

Query: 1117 SGNHKLISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLG 1176
            SGNHKLISLASMLMSKIGLE                         KFFSGQVE+SSPCLG
Sbjct: 1084 SGNHKLISLASMLMSKIGLE-------------------------KFFSGQVESSSPCLG 1143

Query: 1177 EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDG 1236
            EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSH SLL+STFNILLDG
Sbjct: 1144 EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHVSLLNSTFNILLDG 1203

Query: 1237 ALLCGSRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV 1296
            ALLCGSRAAQSIVNI PYPDTLL+DIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV
Sbjct: 1204 ALLCGSRAAQSIVNIWPYPDTLLDDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV 1263

Query: 1297 RRLFIGEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ 1356
            RRLFIGEAVNIDCLRILPLIV+FLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ
Sbjct: 1264 RRLFIGEAVNIDCLRILPLIVNFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ 1323

Query: 1357 RVLLFPSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRK 1416
            RVLLFPSLSQWQAGED EDWLLLVISCYPFSSSM GLHTLKPDRNISTEE SLLLEIFRK
Sbjct: 1324 RVLLFPSLSQWQAGEDAEDWLLLVISCYPFSSSMRGLHTLKPDRNISTEEGSLLLEIFRK 1383

Query: 1417 QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME 1476
            QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME
Sbjct: 1384 QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME 1443

Query: 1477 EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL 1536
            EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL
Sbjct: 1444 EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL 1503

Query: 1537 KDMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHF 1596
            K+METLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTII+SSRIELPHF
Sbjct: 1504 KNMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIISSSRIELPHF 1563

Query: 1597 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE 1656
            WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE
Sbjct: 1564 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE 1623

Query: 1657 PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM 1716
            PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM
Sbjct: 1624 PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM 1683

Query: 1717 AQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK 1776
            A ERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVE MALQK
Sbjct: 1684 AHERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVESMALQK 1743

Query: 1777 KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV 1836
            KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV
Sbjct: 1744 KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV 1803

Query: 1837 RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT 1896
            RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT
Sbjct: 1804 RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT 1863

Query: 1897 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1922
            YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI
Sbjct: 1864 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1904

BLAST of Cp4.1LG02g11280 vs. ExPASy TrEMBL
Match: A0A6J1G9M2 (E3 ubiquitin-protein ligase listerin OS=Cucurbita moschata OX=3662 GN=LOC111452200 PE=3 SV=1)

HSP 1 Score: 3308 bits (8577), Expect = 0.0
Identity = 1727/1982 (87.13%), Postives = 1753/1982 (88.45%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSEFLKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEFLKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFA 336
            Q+    L+                ++     + +R+  TGKGSGETKHASKSRETAISFA
Sbjct: 244  QVISSSLLALAT-----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFA 303

Query: 337  EKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKD 396
            EKLFTEHKYFVHLLQSKSTIVRSATFSV+KSLVKNIPHAFKEQ+MKTISGSILGAFQEKD
Sbjct: 304  EKLFTEHKYFVHLLQSKSTIVRSATFSVVKSLVKNIPHAFKEQNMKTISGSILGAFQEKD 363

Query: 397  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT------------------------------ 456
            PSCHSSMWDAVLLFSKRLPNCWTYVNVQKT                              
Sbjct: 364  PSCHSSMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISYPALILFLDT 423

Query: 457  --------------FHLVLWESRN--FSSTFSRI-----------YGLEGT--------- 516
                          F   LW  RN   SS   R+           +GL+           
Sbjct: 424  VPPSAVGEQKFLLNFFENLWVGRNPFHSSNAERLAFFQAFKECFLWGLQNASRFCSGDDL 483

Query: 517  ---------------------HSIRQMQK------GLRPLNNKRTAETPSTKYPMSYLQD 576
                                 H ++ ++       G  PLNNKRT ETPSTKYPMSYLQD
Sbjct: 484  AHFQVTLVDVILVNLLWKDYLHDVQCLKNQDRALSGGAPLNNKRTGETPSTKYPMSYLQD 543

Query: 577  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 636
            LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ
Sbjct: 544  LRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQ 603

Query: 637  LSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNG 696
            LSMDKDDIWPLA+LVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPR IVRELFICNNG
Sbjct: 604  LSMDKDDIWPLAILVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRNIVRELFICNNG 663

Query: 697  MSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWN 756
             SSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNN SAGARLDLLL LIDDE FSHQWN
Sbjct: 664  KSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNCSAGARLDLLLTLIDDEHFSHQWN 723

Query: 757  SVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 816
            SVISYSF+LDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH
Sbjct: 724  SVISYSFDLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHH 783

Query: 817  EHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 876
            EHLESAAVAIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF
Sbjct: 784  EHLESAAVAIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF 843

Query: 877  LLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYL 936
            LLHSPLSWARNSCS LIPKPGNSFPNSISSSDVVA+AHFSLEVLDRCVFCLYNLGEENYL
Sbjct: 844  LLHSPLSWARNSCSLLIPKPGNSFPNSISSSDVVAIAHFSLEVLDRCVFCLYNLGEENYL 903

Query: 937  LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH 996
            LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH
Sbjct: 904  LPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTH 963

Query: 997  NRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSGLLPTX 1056
            +RKKYGSILIQFIRSAIFS+DTEEIVSLCCQWMLE+KDQISQDHLEEQ            
Sbjct: 964  DRKKYGSILIQFIRSAIFSEDTEEIVSLCCQWMLEVKDQISQDHLEEQ------------ 1023

Query: 1057 EIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHK 1116
                          YML QLLIKGDTWPFWIAPN MA NELA SNTKNIGFDIHKSGNHK
Sbjct: 1024 --------------YMLYQLLIKGDTWPFWIAPNFMASNELATSNTKNIGFDIHKSGNHK 1083

Query: 1117 LISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLGEATNN 1176
            LISLASMLMSKIGLE                         KFFSGQVENSSPCLGEATNN
Sbjct: 1084 LISLASMLMSKIGLE-------------------------KFFSGQVENSSPCLGEATNN 1143

Query: 1177 EVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCG 1236
            EVTCRAWLVAEILCTW WPGGNARGSFLPLFCAYVK+SCSHESLLDSTFNILLDGALLCG
Sbjct: 1144 EVTCRAWLVAEILCTWNWPGGNARGSFLPLFCAYVKKSCSHESLLDSTFNILLDGALLCG 1203

Query: 1237 SRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFI 1296
            SRAAQSIVNI PYPD+LLEDIQEPFLRALASLLF MLKENIWGRDKASSLFELLVRRLFI
Sbjct: 1204 SRAAQSIVNIWPYPDSLLEDIQEPFLRALASLLFCMLKENIWGRDKASSLFELLVRRLFI 1263

Query: 1297 GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1356
            GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF
Sbjct: 1264 GEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLF 1323

Query: 1357 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS 1416
            PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS
Sbjct: 1324 PSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNIS 1383

Query: 1417 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1476
            GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES
Sbjct: 1384 GRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAES 1443

Query: 1477 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1536
            VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET
Sbjct: 1444 VNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMET 1503

Query: 1537 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1596
            LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA
Sbjct: 1504 LNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIA 1563

Query: 1597 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1656
            SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS
Sbjct: 1564 SSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYS 1623

Query: 1657 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERV 1716
            AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMA ERV
Sbjct: 1624 AIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAHERV 1683

Query: 1717 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTE 1776
            NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDC+FQHIPVEGMALQKKKDTE
Sbjct: 1684 NIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCIFQHIPVEGMALQKKKDTE 1743

Query: 1777 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1836
            LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC
Sbjct: 1744 LPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFC 1803

Query: 1837 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1896
            DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE
Sbjct: 1804 DLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDE 1863

Query: 1897 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1922
            TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR
Sbjct: 1864 TGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKR 1899

BLAST of Cp4.1LG02g11280 vs. ExPASy TrEMBL
Match: A0A6J1KCY9 (E3 ubiquitin-protein ligase listerin OS=Cucurbita maxima OX=3661 GN=LOC111493187 PE=3 SV=1)

HSP 1 Score: 3123 bits (8096), Expect = 0.0
Identity = 1622/1854 (87.49%), Postives = 1647/1854 (88.83%), Query Frame = 0

Query: 170  MDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAF 229
            MDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAF
Sbjct: 1    MDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSEVSQSAMQSLQAAF 60

Query: 230  PAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQQLHYQILVFPLEY 289
            PAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQQ+    L+     
Sbjct: 61   PAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQQVISSSLLALAT- 120

Query: 290  KSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSRETAISFAEKLFTEHKYFVHL 349
                       ++     + +R+  TGKGSGETKHASKSRETAISFAEKLFTEHKYFVHL
Sbjct: 121  ----------LIDVLVSVRTERS-GTGKGSGETKHASKSRETAISFAEKLFTEHKYFVHL 180

Query: 350  LQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQEKDPSCHSSMWDAVLL 409
            LQSKSTIVRSATFSVLKSLVKNIPHAFKEQ+MKTISGSILGAFQEKDPSCHSSMWDAVLL
Sbjct: 181  LQSKSTIVRSATFSVLKSLVKNIPHAFKEQNMKTISGSILGAFQEKDPSCHSSMWDAVLL 240

Query: 410  FSKRLPNCWTYVNVQKT------------------------------------------- 469
            FSKRLPNCWTYVNVQKT                                           
Sbjct: 241  FSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQLISHPALILFLDTVPPSAVGEQKFLL 300

Query: 470  -FHLVLWESRN--FSSTFSRI-----------YGLEGT---------------------- 529
             F   LW  RN   SS   R+           +GL+                        
Sbjct: 301  NFFENLWVGRNPFHSSNAERLAFFQAFKECFLWGLQNASRFCSGDDLAHFQVTLVDVILV 360

Query: 530  --------HSIRQMQK------GLRPLNNKRTAETPSTKYPMSYLQDLRKCIVEILSGIY 589
                    H ++ ++       G  PLNNKRTAETPSTKYPMSYLQDLRKCIVEILSGIY
Sbjct: 361  NLLWKDYLHDVQCLKSQDRALSGGAPLNNKRTAETPSTKYPMSYLQDLRKCIVEILSGIY 420

Query: 590  LVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQLSMDKDDIWPLAL 649
            LVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQLSMDKDDIWPLAL
Sbjct: 421  LVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELEQLSMDKDDIWPLAL 480

Query: 650  LVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGMSSTHLSGVEAQD 709
            LVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGMSSTHLSGVEAQD
Sbjct: 481  LVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNNGMSSTHLSGVEAQD 540

Query: 710  VEA-----RQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQWNSVISYSFN 769
            VEA     RQFMQIFNEVFVPWCLQGNNSSAG+RLDLLLALIDDE FSHQWNSVISYSFN
Sbjct: 541  VEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGSRLDLLLALIDDEHFSHQWNSVISYSFN 600

Query: 770  LDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHEHLESAAV 829
            LDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHEHLESAAV
Sbjct: 601  LDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWHHEHLESAAV 660

Query: 830  AIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSFLLHSPLSW 889
            AIAQSQAPFRSSFT+   SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSF+LHSPLSW
Sbjct: 661  AIAQSQAPFRSSFTEFLCSVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVSFILHSPLSW 720

Query: 890  ARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYLLPSILATI 949
            ARNSCS LIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYLLPSILATI
Sbjct: 721  ARNSCSLLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGEENYLLPSILATI 780

Query: 950  YAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTHNRKKYGSI 1009
            YAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTHNRKKYGSI
Sbjct: 781  YAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIYQFWKNCKTHNRKKYGSI 840

Query: 1010 LIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSGLLPTXEIKDQISQ 1069
            LIQFIRSAIFS+DTEEIVSLCCQWMLEIKDQISQDHLEEQ                    
Sbjct: 841  LIQFIRSAIFSEDTEEIVSLCCQWMLEIKDQISQDHLEEQ-------------------- 900

Query: 1070 DHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHKSGNHKLISLASML 1129
                  YMLDQLLIKGDTWPFWIAPN MAPNELAASNTKNIGF IHKSGNHKLISLASML
Sbjct: 901  ------YMLDQLLIKGDTWPFWIAPNFMAPNELAASNTKNIGFHIHKSGNHKLISLASML 960

Query: 1130 MSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLGEATNNEVTCRAWL 1189
            MSKIGLE                         KFFSGQVE+SSPCLGEATNNEVTCRAWL
Sbjct: 961  MSKIGLE-------------------------KFFSGQVESSSPCLGEATNNEVTCRAWL 1020

Query: 1190 VAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDGALLCGSRAAQSIV 1249
            VAEILCTWKWPGGNARGSFLPLFCAYVKRSCSH SLL+STFNILLDGALLCGSRAAQSIV
Sbjct: 1021 VAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHVSLLNSTFNILLDGALLCGSRAAQSIV 1080

Query: 1250 NISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIGEAVNIDC 1309
            NI PYPDTLL+DIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIGEAVNIDC
Sbjct: 1081 NIWPYPDTLLDDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLVRRLFIGEAVNIDC 1140

Query: 1310 LRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLFPSLSQWQA 1369
            LRILPLIV+FLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLFPSLSQWQA
Sbjct: 1141 LRILPLIVNFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQRVLLFPSLSQWQA 1200

Query: 1370 GEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRKQRNISGRSSTVNH 1429
            GED EDWLLLVISCYPFSSSM GLHTLKPDRNISTEE SLLLEIFRKQRNISGRSSTVNH
Sbjct: 1201 GEDAEDWLLLVISCYPFSSSMRGLHTLKPDRNISTEEGSLLLEIFRKQRNISGRSSTVNH 1260

Query: 1430 APRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESVNGIIVNS 1489
            APRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESVNGIIVNS
Sbjct: 1261 APRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVMEEIAESVNGIIVNS 1320

Query: 1490 STSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKDMETLNPQRLDK 1549
            STSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLK+METLNPQRLDK
Sbjct: 1321 STSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGLKNMETLNPQRLDK 1380

Query: 1550 LNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHFWDLIASSVTKSSK 1609
            LNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTII+SSRIELPHFWDLIASSVTKSSK
Sbjct: 1381 LNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIISSSRIELPHFWDLIASSVTKSSK 1440

Query: 1610 DARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSAIIRENTS 1669
            DARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSAIIRENTS
Sbjct: 1441 DARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTEPISYSAIIRENTS 1500

Query: 1670 CYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAQERVNIFLAWSL 1729
            CYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMA ERVNIFLAWSL
Sbjct: 1501 CYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELMAHERVNIFLAWSL 1560

Query: 1730 LLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQKKKDTELPAGLSEA 1789
            LLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVE MALQKKKDTELPAGLSEA
Sbjct: 1561 LLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVESMALQKKKDTELPAGLSEA 1620

Query: 1790 ATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCDLRDRSKS 1849
            ATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCDLRDRSKS
Sbjct: 1621 ATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYVRGWFCDLRDRSKS 1680

Query: 1850 SVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIR 1909
            SVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIR
Sbjct: 1681 SVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIATYTKDETGMDLVIR 1740

Query: 1910 LPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEG 1922
            LPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEG
Sbjct: 1741 LPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAIRIWKRNFDKEFEG 1791

BLAST of Cp4.1LG02g11280 vs. ExPASy TrEMBL
Match: A0A1S3C4S0 (E3 ubiquitin-protein ligase listerin OS=Cucumis melo OX=3656 GN=LOC103497027 PE=3 SV=1)

HSP 1 Score: 2912 bits (7549), Expect = 0.0
Identity = 1535/1987 (77.25%), Postives = 1638/1987 (82.44%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDS+LTGDDAA FSDID E
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSSLTGDDAAPFSDIDSE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSE LKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEILKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVR ATH+TMTNLV+AAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDAL+LCTTEIFIYLEENLKLTPDTLSDK+VAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALVLCTTEIFIYLEENLKLTPDTLSDKLVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHAS--KSRETAIS 336
            Q+    L+          S   E               TGK SGETKH S  +SRETAIS
Sbjct: 244  QVISSSLLALATLIDVLVSGRSERSG------------TGKSSGETKHTSMSRSRETAIS 303

Query: 337  FAEKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQE 396
            FAEKLFTEHKYF+ LL+SKS IVRSAT+SV++SLVKNIPHAFKEQ+MKTI+GSILGAFQE
Sbjct: 304  FAEKLFTEHKYFIDLLKSKSNIVRSATYSVMRSLVKNIPHAFKEQNMKTIAGSILGAFQE 363

Query: 397  KDPSCHSSMWDAVLLFSKRLPNCWTYVNVQKT---------------------------- 456
            KDPSCHS MWDAVLLFSKRLPNCWTYVNVQKT                            
Sbjct: 364  KDPSCHSPMWDAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQKISYPTLILFL 423

Query: 457  ----------------FHLVLWESRN--FSSTFSRI-----------YGLEGTHS----- 516
                            F   LW  RN   SS+  R+           +G++   S     
Sbjct: 424  DTVPPCAVGGEKFLLDFFENLWVGRNPFHSSSTERLAFFQAFKECFLWGIQNASSFCNGD 483

Query: 517  --------------IRQMQKGL----------------RPLNNKRTAETPSTKYPMSYLQ 576
                          ++ + K                   PLNNK T + PSTKYPMSYLQ
Sbjct: 484  DFVHFQVTLIDAILVKLLWKDYLHVQCLKNQDRVFSEDAPLNNKMTEDLPSTKYPMSYLQ 543

Query: 577  DLRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELE 636
            DLRKCIVEILS I+L +HDLLSVFA EFQKNCI MFQLTDN  VAS TIEQIIGFILELE
Sbjct: 544  DLRKCIVEILSSIHLAKHDLLSVFAMEFQKNCIDMFQLTDNVGVASETIEQIIGFILELE 603

Query: 637  QLSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNN 696
            QLSMDKDD W L  LVGPTLAN FPII+S +S DGVRLLSAA+SVFGPRKIVRELFI NN
Sbjct: 604  QLSMDKDDTWLLVHLVGPTLANTFPIIQSLDSLDGVRLLSAAVSVFGPRKIVRELFINNN 663

Query: 697  GMSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQW 756
            GMSST  SGVEAQD+EARQFMQ+FN++FVPWCLQGNNSS+ A+LDLLLALIDDE FS QW
Sbjct: 664  GMSSTKFSGVEAQDLEARQFMQVFNDIFVPWCLQGNNSSSSAQLDLLLALIDDEHFSDQW 723

Query: 757  NSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWH 816
            +SVISYS NL+HT VVLES+NSESLA+LAKLL+R RGKITNSDAR VT+TWQ+ANLGNWH
Sbjct: 724  HSVISYSTNLNHTEVVLESMNSESLAVLAKLLNRVRGKITNSDARKVTHTWQRANLGNWH 783

Query: 817  HEHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVS 876
            HEHLESAA+AIAQS AP RSSFTD   SVLGGSVQ+DCSSFVSRD LIAIFEA+FQKLVS
Sbjct: 784  HEHLESAAIAIAQSHAPIRSSFTDFLCSVLGGSVQNDCSSFVSRDALIAIFEALFQKLVS 843

Query: 877  FLLHSPLSWARNSCSSLIPKPGNS---FPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGE 936
            FLLHSP +WARNSCS LI +P +    FP   +SS+VV MA+F+LEVLDRC+FCLYNLGE
Sbjct: 844  FLLHSPFTWARNSCSLLISRPDSPEKIFPK-YTSSEVVVMANFALEVLDRCIFCLYNLGE 903

Query: 937  ENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIY-QFWK 996
            ENYLLPSILATIYAIDWDCS+E +QDD+LD+KFKEE + RLLFG  VR LRQKI  QFWK
Sbjct: 904  ENYLLPSILATIYAIDWDCSMEGKQDDVLDEKFKEESKARLLFGESVRALRQKITDQFWK 963

Query: 997  NCKTHNRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSG 1056
            +C+THNRKKYGSILIQFIRSAIFS+D+EEIVSLC QWMLEI D ISQD  EEQ       
Sbjct: 964  SCRTHNRKKYGSILIQFIRSAIFSEDSEEIVSLCLQWMLEILDHISQDQFEEQ------- 1023

Query: 1057 LLPTXEIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHK 1116
                               YMLDQLLIK DTWPFWIAP+ MAPNE AASNTKNIG DIH 
Sbjct: 1024 -------------------YMLDQLLIKNDTWPFWIAPDFMAPNEFAASNTKNIGLDIHI 1083

Query: 1117 SGNHKLISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLG 1176
            SGNHK ISL SM MSKIGLE  FSGQVENSSPC++ M                       
Sbjct: 1084 SGNHKFISLISMFMSKIGLEKLFSGQVENSSPCISKM----------------------- 1143

Query: 1177 EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDG 1236
              T NEVT RAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFN+LLDG
Sbjct: 1144 --TKNEVTSRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDG 1203

Query: 1237 ALLCGSRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV 1296
            ALL  SRAAQS++NI PYP +LLEDIQEPFLRALASLLFS+LKENIWGRDKASS FELLV
Sbjct: 1204 ALLYSSRAAQSLINIWPYPVSLLEDIQEPFLRALASLLFSLLKENIWGRDKASSQFELLV 1263

Query: 1297 RRLFIGEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ 1356
             RLFIGEAVNIDCLRILPLI+S+L+RPMCERN+ FDD GSCS +   EN  Q T EGWLQ
Sbjct: 1264 SRLFIGEAVNIDCLRILPLILSYLVRPMCERNSTFDDCGSCSGDSLMENTFQRTTEGWLQ 1323

Query: 1357 RVLLFPSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRK 1416
            RVLLFPSL++WQ G+DME WLLLVISCYPFS S+GGL TLK DRNISTEE SLLLE+FRK
Sbjct: 1324 RVLLFPSLNEWQLGQDMEYWLLLVISCYPFSCSIGGLQTLKLDRNISTEEGSLLLELFRK 1383

Query: 1417 QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME 1476
            QR  S RS  VNHAP VQMLLSELMVVSVGYCWKQF+ EDWEFLL QLMSWIQ AVV+ME
Sbjct: 1384 QRKASSRSPAVNHAPWVQMLLSELMVVSVGYCWKQFSHEDWEFLLFQLMSWIQSAVVIME 1443

Query: 1477 EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL 1536
            EIAESVN IIV SST+M+ NEILEKLEQSV I DPIPFCISRNALLSFSLF GS GL GL
Sbjct: 1444 EIAESVNDIIVKSSTAMDLNEILEKLEQSVLILDPIPFCISRNALLSFSLFDGSLGLHGL 1503

Query: 1537 KDMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHF 1596
            KDME+ +PQ+ DKLNHVNDRIVEGILRMFFCTGISEAI  S+ DKA +II+SSR+ELP+F
Sbjct: 1504 KDMESSSPQQFDKLNHVNDRIVEGILRMFFCTGISEAIAYSFSDKAASIISSSRLELPYF 1563

Query: 1597 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE 1656
            WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFS KP+PSLQYAAY MLSTE
Sbjct: 1564 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSPKPIPSLQYAAYVMLSTE 1623

Query: 1657 PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM 1716
            PIS SAIIRENTSCYLDYD TTEQ STQVDFSSEYNVLLKEEI+ MIEKLPDDVF+MEL+
Sbjct: 1624 PISNSAIIRENTSCYLDYDITTEQRSTQVDFSSEYNVLLKEEILCMIEKLPDDVFEMELI 1683

Query: 1717 AQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK 1776
            AQERVNI+LAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK
Sbjct: 1684 AQERVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK 1743

Query: 1777 KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV 1836
            +KDTELPAGLSEAATAANQAITTGSLLF VEFLWP+EPVKLAS+AGAI+GLMLRVLPAYV
Sbjct: 1744 RKDTELPAGLSEAATAANQAITTGSLLFSVEFLWPIEPVKLASFAGAIFGLMLRVLPAYV 1803

Query: 1837 RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT 1896
            RGWF DLRDRSKSSV+ESFTKAWCSPS+IANELSQIKKAEFADENFSVVVSKSANEVIAT
Sbjct: 1804 RGWFSDLRDRSKSSVLESFTKAWCSPSIIANELSQIKKAEFADENFSVVVSKSANEVIAT 1863

Query: 1897 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1922
            YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI
Sbjct: 1864 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1903

BLAST of Cp4.1LG02g11280 vs. ExPASy TrEMBL
Match: A0A0A0LXT2 (E3 ubiquitin-protein ligase listerin OS=Cucumis sativus OX=3659 GN=Csa_1G533710 PE=3 SV=1)

HSP 1 Score: 2905 bits (7530), Expect = 0.0
Identity = 1533/1987 (77.15%), Postives = 1638/1987 (82.44%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLD +LTGDDAA FSDIDGE
Sbjct: 4    PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDYSLTGDDAAPFSDIDGE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHLKRLSRKDPTTK              LKALASLSE LKQKSGKDVASIIPQW    
Sbjct: 64   VAQHLKRLSRKDPTTK--------------LKALASLSEILKQKSGKDVASIIPQW---- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                 VFEYKKLLMDYNRDVR ATH+TMTNLV+AAGREIAPHLKSLMGPWWFSQFDSVSE
Sbjct: 124  -----VFEYKKLLMDYNRDVRRATHDTMTNLVMAAGREIAPHLKSLMGPWWFSQFDSVSE 183

Query: 217  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSDKVVAKDELEEMHQ 276
            VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLS+KVVAKDELEEMHQ
Sbjct: 184  VSQSAMQSLQAAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSEKVVAKDELEEMHQ 243

Query: 277  QLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKGSGETKHASKSR--ETAIS 336
            Q+    L+          S   E               TGK SGETKHASKSR  ETAIS
Sbjct: 244  QVISSSLLALATLIDVLVSGRSERSG------------TGKSSGETKHASKSRSRETAIS 303

Query: 337  FAEKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSMKTISGSILGAFQE 396
            FAEKLFTEHKYF+ LL+SKS IVR AT+SV++SLVKNIPHAFKEQ+MKTI+GSILGAFQE
Sbjct: 304  FAEKLFTEHKYFIDLLKSKSNIVRFATYSVMRSLVKNIPHAFKEQNMKTIAGSILGAFQE 363

Query: 397  KDPSCHSSMWDAVLLFSKRLPNCWTYVNVQKT---------------------------- 456
            KDPSCHS MW+AVLLFSKRLPNCWTYVNVQKT                            
Sbjct: 364  KDPSCHSPMWEAVLLFSKRLPNCWTYVNVQKTVLNRFWNFLRNGCFGSQKISYPTLILFL 423

Query: 457  ----------------FHLVLWESRN--FSSTFSRI-----------YGLEGTHS----- 516
                            F   LW  RN   SS+  R+           +G++   S     
Sbjct: 424  DTVPPRAVGGEKFLLDFFDNLWVGRNPFHSSSTERLAFFQAFKECFLWGIQNASSFCNGD 483

Query: 517  --------------IRQMQKGL----------------RPLNNKRTAETPSTKYPMSYLQ 576
                          ++ + K                   PLNNK   + PSTKYPMSYLQ
Sbjct: 484  DFAHFQVTLVDAILVKILWKDYLHVQCLKNQDRVFSEDEPLNNKMIEDIPSTKYPMSYLQ 543

Query: 577  DLRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGFILELE 636
            DLRKCIVEILS I+LV+HDLLSVFA EFQKNC+ MFQLTDN  VAS TIEQIIGFILELE
Sbjct: 544  DLRKCIVEILSSIHLVKHDLLSVFAMEFQKNCLDMFQLTDNVGVASETIEQIIGFILELE 603

Query: 637  QLSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVRELFICNN 696
            QLSMDKDD W L  LVGPTLAN FPII+S +SSDGVRLLSAA+SVFGPRKIV+ELFI NN
Sbjct: 604  QLSMDKDDTWLLVHLVGPTLANTFPIIQSLDSSDGVRLLSAAVSVFGPRKIVQELFINNN 663

Query: 697  GMSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDERFSHQW 756
            GMSST  SGVEAQD+EARQFMQ+FN+VFVPWCLQGNNSS+ ARLDLLLALIDDE FS QW
Sbjct: 664  GMSSTEFSGVEAQDLEARQFMQVFNDVFVPWCLQGNNSSSSARLDLLLALIDDEHFSDQW 723

Query: 757  NSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKANLGNWH 816
            +S+ISYS NLDHT VVLES+NSESLA+LAKLL+R RGKITNSDAR VT+TWQ+ANLGNWH
Sbjct: 724  HSIISYSTNLDHTEVVLESMNSESLAVLAKLLNRVRGKITNSDARKVTHTWQRANLGNWH 783

Query: 817  HEHLESAAVAIAQSQAPFRSSFTD---SVLGGSVQSDCSSFVSRDTLIAIFEAVFQKLVS 876
            HEHLESAAVAIAQS +P RSSFTD   SVLGGSVQ+DCSSFVSRD LIAIFEA+FQKLVS
Sbjct: 784  HEHLESAAVAIAQSHSPIRSSFTDFVCSVLGGSVQNDCSSFVSRDALIAIFEALFQKLVS 843

Query: 877  FLLHSPLSWARNSCSSLIPKPGN---SFPNSISSSDVVAMAHFSLEVLDRCVFCLYNLGE 936
            FLLHSPL+WARNSCS LI +P     SFP   SSS+VV MA+F+LEVLDRC FCL +LGE
Sbjct: 844  FLLHSPLTWARNSCSLLISRPDYPEISFPKYTSSSEVVVMANFALEVLDRCFFCLCHLGE 903

Query: 937  ENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKIY-QFWK 996
            ENYLLPSILATIYAIDWDCS+E +QDDMLD+KFKEE + RL+FG  VR LRQKI  +FW 
Sbjct: 904  ENYLLPSILATIYAIDWDCSMEGKQDDMLDEKFKEESKARLVFGESVRALRQKITDKFWN 963

Query: 997  NCKTHNRKKYGSILIQFIRSAIFSDDTEEIVSLCCQWMLEIKDQISQDHLEEQVIHGLSG 1056
            +C TH+RKKYGSILIQFIRSAIFS+D+EEIVSLC QWMLEI DQISQD  EEQ       
Sbjct: 964  SCTTHHRKKYGSILIQFIRSAIFSEDSEEIVSLCFQWMLEILDQISQDQFEEQ------- 1023

Query: 1057 LLPTXEIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIGFDIHK 1116
                               YMLDQLLIK DTWPFWIAPN MAPNELAASNTKN+G DIHK
Sbjct: 1024 -------------------YMLDQLLIKTDTWPFWIAPNFMAPNELAASNTKNVGLDIHK 1083

Query: 1117 SGNHKLISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENSSPCLG 1176
            SGNHK ISL SM MSKIGLE  F+ QVENSS C++ M                       
Sbjct: 1084 SGNHKFISLISMFMSKIGLEKLFNVQVENSSTCISKM----------------------- 1143

Query: 1177 EATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNILLDG 1236
              T NEVT RAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFN+LLDG
Sbjct: 1144 --TKNEVTSRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFNMLLDG 1203

Query: 1237 ALLCGSRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSLFELLV 1296
            ALL  SRAAQS +NI PYP +LLEDIQEPFLRALASLLFS+L+ENIWGRDKA S FELLV
Sbjct: 1204 ALLYSSRAAQSFINIWPYPVSLLEDIQEPFLRALASLLFSLLEENIWGRDKAISQFELLV 1263

Query: 1297 RRLFIGEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENIIQNTIEGWLQ 1356
             RLFIGEAVNIDCLRILPLI+S+L+RPMCERN+ FDDSGSCS +   EN  Q+TIEGWLQ
Sbjct: 1264 SRLFIGEAVNIDCLRILPLILSYLVRPMCERNSTFDDSGSCSGDSLMENTFQSTIEGWLQ 1323

Query: 1357 RVLLFPSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSLLLEIFRK 1416
            RVLLFPSL++WQ G+DME WLLLVISCYPFS ++GGL TLK DRNISTEE SLLLE+FRK
Sbjct: 1324 RVLLFPSLNEWQLGQDMEYWLLLVISCYPFSCTIGGLQTLKLDRNISTEEGSLLLELFRK 1383

Query: 1417 QRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQPAVVVME 1476
            QR  SGRS   NHAP VQMLLSELMVVSVGYCWKQF+DEDWEFLL QLMS IQ AVV+ME
Sbjct: 1384 QRKASGRSPAGNHAPWVQMLLSELMVVSVGYCWKQFSDEDWEFLLFQLMSGIQSAVVIME 1443

Query: 1477 EIAESVNGIIVNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLFYGSFGLQGL 1536
            EIAESVN IIV SST+M+ NEILEKLEQSV IS+PIPFCISRNALLSFSLF GS GL GL
Sbjct: 1444 EIAESVNDIIVKSSTTMDLNEILEKLEQSVLISNPIPFCISRNALLSFSLFDGSLGLHGL 1503

Query: 1537 KDMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIASSRIELPHF 1596
            KD+E+ +PQ+ DKLNHVNDRIVEGILRMFFCTGISEAI CS+ DKA +II+SSR+ELP+F
Sbjct: 1504 KDLESSSPQQFDKLNHVNDRIVEGILRMFFCTGISEAIACSFSDKAASIISSSRLELPYF 1563

Query: 1597 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQYAAYFMLSTE 1656
            WDLIASSVTKSSKDARERAVKSIEFWGLSKGP+SSLYGILFS KPVPSLQYAAY MLSTE
Sbjct: 1564 WDLIASSVTKSSKDARERAVKSIEFWGLSKGPISSLYGILFSPKPVPSLQYAAYVMLSTE 1623

Query: 1657 PISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLPDDVFDMELM 1716
            PIS SAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEI+ MIEKLPDDVFDMEL+
Sbjct: 1624 PISNSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEILCMIEKLPDDVFDMELI 1683

Query: 1717 AQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK 1776
            AQERVNI+LAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK
Sbjct: 1684 AQERVNIYLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHIPVEGMALQK 1743

Query: 1777 KKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAIYGLMLRVLPAYV 1836
            +KDTE PAGLSEAATAANQAITTGSLLF VEFLWP+EPVKLA++AGAI+GLMLRVLPAYV
Sbjct: 1744 RKDTEQPAGLSEAATAANQAITTGSLLFSVEFLWPIEPVKLATFAGAIFGLMLRVLPAYV 1803

Query: 1837 RGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSVVVSKSANEVIAT 1896
            RGWF DLRDRSKSS +ESFTK WCSPSLI NELSQIKKAEFADENFSVVVSKSANEVIAT
Sbjct: 1804 RGWFSDLRDRSKSSALESFTKVWCSPSLITNELSQIKKAEFADENFSVVVSKSANEVIAT 1863

Query: 1897 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1922
            YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI
Sbjct: 1864 YTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSFVRNQNGALAEAI 1904

BLAST of Cp4.1LG02g11280 vs. TAIR 10
Match: AT5G58410.1 (HEAT/U-box domain-containing protein )

HSP 1 Score: 1519.6 bits (3933), Expect = 0.0e+00
Identity = 894/1998 (44.74%), Postives = 1222/1998 (61.16%), Query Frame = 0

Query: 37   PKGDGARSKARPSSSSLAASLLPSDSAANAAGFGGFLGSYRLDSTLTGDDAASFSDIDGE 96
            PKGD ARSKARPSSSSLAASLLPS SAA A GFGG++GS R  ++L+ +D+ASF D+D E
Sbjct: 4    PKGDAARSKARPSSSSLAASLLPSGSAA-AVGFGGYVGSSRFQTSLSNEDSASFLDLDSE 63

Query: 97   VAQHLKRLSRKDPTTKGSLIGTNLLLDFQQLKALASLSEFLKQKSGKDVASIIPQWTIAC 156
            VAQHL+RLSRKDPTTK              +KALASLSE +KQK GK++  IIPQWT   
Sbjct: 64   VAQHLQRLSRKDPTTK--------------IKALASLSELVKQKQGKELLPIIPQWT--- 123

Query: 157  LEFWKVFEYKKLLMDYNRDVRLATHETMTNLVIAAGREIAPHLKSLMGPWWFSQFDSVSE 216
                  FEYKKL++DY+RDVR ATH+ MTN+V  AGR+IAPHLKS+MGPWWFSQFD  SE
Sbjct: 124  ------FEYKKLILDYSRDVRRATHDVMTNVVTGAGRDIAPHLKSIMGPWWFSQFDLASE 183

Query: 217  VSQSAMQSLQ-------------AAFPAQEKRVDALILCTTEIFIYLEENLKLTPDTLSD 276
            VSQ+A  S Q             AAFPAQEKR+ AL LC+ EIF YLEENLKLTP  LSD
Sbjct: 184  VSQAAKSSFQVGSSFGNSVFLVEAAFPAQEKRLHALNLCSAEIFAYLEENLKLTPQNLSD 243

Query: 277  KVVAKDELEEMHQQLHYQILVFPLEYKSEHYSEEKEYMNFRSKTQKQRAQXTGKG--SGE 336
            K +A DELEEM+QQ+    LV  L    +    E +               TG    + E
Sbjct: 244  KSLASDELEEMYQQMISSSLV-GLATLLDILLREPD--------------NTGSANINSE 303

Query: 337  TKHASKSRETAISFAEKLFTEHKYFVHLLQSKSTIVRSATFSVLKSLVKNIPHAFKEQSM 396
            +K ASK+R  A S AEK+F+ HK F++ L+S+S  +RSAT+S+L S +KN+P  F E  +
Sbjct: 304  SKLASKARAVATSSAEKMFSSHKCFLNFLKSESPSIRSATYSLLSSFIKNVPEVFGEGDV 363

Query: 397  KTISGSILGAFQEKDPSCHSSMWDAVLLFSKRLPNCWTYVNVQKTFHLVLW--------- 456
            ++++ ++LG F+E +P+CHSSMW+AVLLFSK+ P  W Y+NV K+    LW         
Sbjct: 364  RSLAPALLGVFRENNPTCHSSMWEAVLLFSKKFPQSWVYLNVHKSVLNHLWQFLRNGCYG 423

Query: 457  ---------------------ESRNFSSTFSR---------------------------I 516
                                 ES  F   F +                           +
Sbjct: 424  SPQVSYPALILFLEVMPAQSVESDKFFVNFFKNLLAGRSMCESSSTDQLSLLRATTECFL 483

Query: 517  YGLEGT-------HSIRQMQ--------------------KGLRPLNNKRTAETPSTKYP 576
            +GL          +SI  +Q                    KG  P N +++AE       
Sbjct: 484  WGLRNASRYCDVPNSIHDLQVDLIDKVLVKILWADFTELSKGSIPPNQRKSAENLGMGNS 543

Query: 577  MSYLQDLRKCIVEILSGIYLVEHDLLSVFATEFQKNCISMFQLTDNTEVASGTIEQIIGF 636
            +SYLQ+L +CI+EILSGI L+E +LLS F    Q++ ++M Q  D  E+ +G++ ++I F
Sbjct: 544  VSYLQELGRCILEILSGINLLEQNLLSFFCKAVQESFLNMLQQGD-LEIVAGSMRKMIDF 603

Query: 637  ILELEQLSMDKDDIWPLALLVGPTLANAFPIIRSHESSDGVRLLSAAISVFGPRKIVREL 696
            +L LE+ S+ + + WPL   +GP L+ AFP IRS E  DGV+LLS ++SVFGPRK+V  L
Sbjct: 604  LLLLERYSVLEGESWPLHQFMGPLLSKAFPWIRSSELLDGVKLLSVSVSVFGPRKVVPVL 663

Query: 697  FICNNGMSSTHLSGVEAQDVEARQFMQIFNEVFVPWCLQGNNSSAGARLDLLLALIDDER 756
               ++  +ST LS  + +++   + +++F E+F+PWC+ G +SS  AR DLL +L+DDE 
Sbjct: 664  I--DDIETSTLLSVEKEKNMSPEKLIKVFQEIFIPWCMDGYDSSTAARQDLLFSLLDDEC 723

Query: 757  FSHQWNSVISYSFNLDHTGVVLESINSESLAMLAKLLDRARGKITNSDARNVTNTWQKAN 816
            F+ QW+ VISY FN  H G         +LA +  LL++AR +IT   +    N    + 
Sbjct: 724  FTQQWSDVISYVFNQQHQGF-------NNLAAMKMLLEKARDEITKRSSGQELNQRIGSR 783

Query: 817  LGNWHHEHLESAAVAIAQSQAPFRSS---FTDSVLGGSVQSDCSSFVSRDTLIAIFEAVF 876
              +WHH  +ES A+++  S +   +S   F  SVLGGS Q    SFVSR +L+ I+  + 
Sbjct: 784  PEHWHHTLIESTAISLVHSSSATTTSAVQFLCSVLGGSTQDSSISFVSRSSLVLIYRGIL 843

Query: 877  QKLVSFLLHSPLSWARNSCSSLIPKPGNSFPNSISSSDVVAMAHFSLEVLDRCVFCLYNL 936
            +KL+SF+  SPL    ++CSSLI +   +F +S SS DV+ +A F+ EV+D   F L +L
Sbjct: 844  EKLLSFIKQSPLCSVNDTCSSLIVE-AIAFDSS-SSVDVIVVAKFAAEVIDGSFFSLKSL 903

Query: 937  GEENYLLPSILATIYAIDWDCSIEERQDDMLDDKFKEERRERLLFGGRVRVLRQKI-YQF 996
             ++  LL ++L++I+ ID +  +    D+ L +  KE+R++R      V  +  K+  QF
Sbjct: 904  SQDATLLTTVLSSIFIIDLENRMTSLVDNTLSES-KEKRKDRNFVCDYVHAVCSKMDNQF 963

Query: 997  WKNCKTHNRKKYGSILIQFIRSAIFSDD---TEEIVSLCCQWMLEIKDQISQDHLEEQVI 1056
            WK+     RK   S L QF+RS +  +D     E+  LC   M E+ + +S D  +E+ I
Sbjct: 964  WKSINYDVRKSSASTLAQFLRSVVLLEDDLQPFELTLLCASRMTEVLEYLSLDQSDEENI 1023

Query: 1057 HGLSGLLPTXEIKDQISQDHLEEQYMLDQLLIKGDTWPFWIAPNLMAPNELAASNTKNIG 1116
             GL                          LL++ D WP W++P+  A  +      +   
Sbjct: 1024 CGL--------------------------LLLESDAWPIWVSPSSSASIDTHGMPVQLC- 1083

Query: 1117 FDIHKSGNHKLISLASMLMSKIGLENFFSGQVENSSPCLASMLMSKIGLEKFFSGQVENS 1176
             ++ KS + + +S    L+ K+G+  F  G  ++                  F+ Q    
Sbjct: 1084 -ELRKSKSQRYVSFIDSLIMKLGIHRFIVGHKDHG-----------------FASQ---- 1143

Query: 1177 SPCLGEATNNEVTCRAWLVAEILCTWKWPGGNARGSFLPLFCAYVKRSCSHESLLDSTFN 1236
                           AWL  EILCTW+WPGG  + SFLP   ++ K   S   LL+S F+
Sbjct: 1144 ---------------AWLSVEILCTWEWPGGKVQTSFLPNLVSFCKDEPSSGGLLNSIFD 1203

Query: 1237 ILLDGALLCGSRAAQSIVNISPYPDTLLEDIQEPFLRALASLLFSMLKENIWGRDKASSL 1296
            ILL+GAL+      + + N+    +  + D+ EPFLRAL S L  + KE++WG ++A + 
Sbjct: 1204 ILLNGALVHVKDEEEGLGNMWVDFNNNIVDVVEPFLRALVSFLHILFKEDLWGEEEAMAA 1263

Query: 1297 FELLVRRLFIGEAVNIDCLRILPLIVSFLIRPMCERNTVFDDSGSCSVEGSKENI--IQN 1356
            F+++  +LFIGE  + +CLRI+P I+S +I P+    T     GS      K+ +  ++ 
Sbjct: 1264 FKMITDKLFIGEETSKNCLRIIPYIMSIIISPL---RTKVKSGGS-----GKDTLLPLEV 1323

Query: 1357 TIEGWLQRVLLFPSLSQWQAGEDMEDWLLLVISCYPFSSSMGGLHTLKPDRNISTEESSL 1416
             +  WL+R L FP L  WQ+GED++DW  LVISCYP S        L+  R++STEE +L
Sbjct: 1324 LLRNWLERSLSFPPLVLWQSGEDIQDWFQLVISCYPVSDKAEEAKELQ--RHLSTEERTL 1383

Query: 1417 LLEIFRKQRNISGRSSTVNHAPRVQMLLSELMVVSVGYCWKQFNDEDWEFLLCQLMSWIQ 1476
            LL++FRKQ+   G S+ V   P VQ+LL+ L++++V YC   FN++DW+F+   L   IQ
Sbjct: 1384 LLDLFRKQKQDPGASTVVTQLPAVQILLARLIMIAVSYCGNDFNEDDWDFVFSNLKRLIQ 1443

Query: 1477 PAVVVMEEIAESVNGII--VNSSTSMNANEILEKLEQSVKISDPIPFCISRNALLSFSLF 1536
             AVVVMEE +E+VN  I  V+S      N+ LE L   V ISDP     ++NAL +FSL 
Sbjct: 1444 SAVVVMEETSENVNDFISGVSSMEKEKENDTLEGLGHIVFISDP-SINSAQNALSAFSLL 1503

Query: 1537 YGSFGLQGLKDMETLNPQRLDKLNHVNDRIVEGILRMFFCTGISEAIVCSYGDKATTIIA 1596
                  + ++  + L     +  + V DRI+EG+LR+FFCTG++EAI  SY  +A +I+A
Sbjct: 1504 NALVNHKSVEGEDNLKSLADETWDPVKDRILEGVLRLFFCTGLTEAIAASYSPEAASIVA 1563

Query: 1597 SSRIELPHFWDLIASSVTKSSKDARERAVKSIEFWGLSKGPVSSLYGILFSTKPVPSLQY 1656
            S R++   FW+L+A  V  SS  AR+RAV+++EFWGLS+G +SSLY I+FS+ P+PSLQ 
Sbjct: 1564 SFRVDHLQFWELVAHLVVDSSPRARDRAVRAVEFWGLSRGSISSLYAIMFSSNPIPSLQL 1623

Query: 1657 AAYFMLSTEPISYSAIIRENTSCYLDYDTTTEQGSTQVDFSSEYNVLLKEEIVFMIEKLP 1716
            AAY +LSTEPIS  AI+ +  +  L+ ++  +Q S+     SE  +LL++E+  M+EKL 
Sbjct: 1624 AAYTVLSTEPISRLAIVAD-LNAPLNDESLNDQDSSNAGLPSEDKLLLRDEVSCMVEKLD 1683

Query: 1717 DDVFDMELMAQERVNIFLAWSLLLSHLWSLPPSSSARERLVQYIQNSASSRILDCLFQHI 1776
             ++ D +L A ERV  FLAWSLLLS++ SLP  +  RERLVQYI+ +A+  ILD LFQHI
Sbjct: 1684 HELLDTDLTAPERVQTFLAWSLLLSNVNSLPSLTQGRERLVQYIEKTANPLILDSLFQHI 1743

Query: 1777 PVE---GMALQKKKDTELPAGLSEAATAANQAITTGSLLFLVEFLWPVEPVKLASYAGAI 1836
            P+E   G +L KKKD ++P+ LS  A+AA +AI TGS L  VE LWP+E  K+AS AGAI
Sbjct: 1744 PLELYMGQSL-KKKDGDIPSELSVVASAATRAIITGSSLSTVESLWPIETGKMASLAGAI 1803

Query: 1837 YGLMLRVLPAYVRGWFCDLRDRSKSSVIESFTKAWCSPSLIANELSQIKKAEFADENFSV 1896
            YGLMLRVLPAYVR WF ++RDRS SS+IE+FT+ WCSPSLI NELSQIKKA+F DE+FSV
Sbjct: 1804 YGLMLRVLPAYVREWFSEMRDRSASSLIEAFTRTWCSPSLIKNELSQIKKADFNDESFSV 1863

Query: 1897 VVSKSANEVIATYTKDETGMDLVIRLPSSYPLRHVDVDCMRSLGISEVKQRKWLLSMMSF 1922
             +SK+ANEV+ATYTKDETGMDLVIRLP SYPL+ VDV+C +S+GISE KQRKWL+SM  F
Sbjct: 1864 SISKAANEVVATYTKDETGMDLVIRLPVSYPLKPVDVNCAKSIGISEAKQRKWLMSMQMF 1872

BLAST of Cp4.1LG02g11280 vs. TAIR 10
Match: AT1G67856.1 (RING/U-box superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 3.4e-04
Identity = 19/28 (67.86%), Postives = 23/28 (82.14%), Query Frame = 0

Query: 1893 TCKHKFHSACLYKWFSTSHKSTCPLCQS 1921
            +CKH FHSACL KWF  +H +TCPLC+S
Sbjct: 105  SCKHYFHSACLDKWFGNNH-TTCPLCRS 131