Cp4.1LG02g10200 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g10200
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	flavonol synthase/flavanone 3-hydroxylase-like
Location	Cp4.1LG02: 9768792 .. 9770552 (+)
RNA-Seq Expression	Cp4.1LG02g10200
Synteny	Cp4.1LG02g10200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACATCACTCTGCAACTTAAGAACTCCTTCGAAAATGGCTAACACAGGCATCCCTACTGTGGACGTTTCTCTGTTCTTAAGTGAAGGTGAAAACGAAGCCAAAAAACAGGCCCTTCAAACCATAACTGATGCTTGTTCAAGCTATGGCTTCTTTCAAATTGTGAACCATGGAATCCCCGTTGAGTTTCTTAAAGAAGCTCTGCAACTGTCAAAAACATTTTTCCACTACCCTGATGAGATAAAGCTTCAATATAGCCCCAAGCCTGGGGCTCCCCTTCTTGCTGGCTTCAACAAGCAGAAGACAAATTGTGTAGACAAGAATGAATATGTCCTCATTTTCCCTCCTGGATCCAAATTCAACATCTACCCACAAGAGCCACCACAATTCAAGTGAGTTTTTTGAGATTTGGGTTCTGTTCTTTTGATCTTGCATGTTATGTTGGATTCAAAATGGTTTGATATGAGTATTATAAACATTTTGGTTGTGGCAGGGAAACGCTGGAAGAGATGTTCCTGAAACTAAACGATGTGTCCTTGGTTATAGAGAGCATATTGAATGAGTGTTTGGGGCTTGCTCCTGGTTTCCTCAAACAATTCAACAATGACAGAAGCTGGGACTTCATGACTAATCTCTACTATTTCCCTGCAGCTGATGTGGGAGAAAATGGCCTCATTCACCATGAAGATGCAAACTGCATCACTTTGGTGATCCAGGATGATGCTGGAGGCCTTCAAGTCCAGAAGGACAGCGAATGGATTCCAGTCACCCCTGTTGAAGGAGCTATTGTTGTGAACGTGGGAGATATGATTCAGGTAAAAAAAAACACTGTTGTTAAACATTGAAGGAGCCATTGGGCTAGTGAGAGATCTGTTTGTTTGAAAGATTTTGTTTTAATGAAGGTGTTGAGCAACAAGAAATTCAAGAGCGCCACACACAGAGTGGTGAGGCAAAAGGGAAAAGAGAGATATTCTTTTGCATTCTTCCGCAGCTTGCATGGAGACAAATGGGTGGAGCCATTGCCAGAGTTCACTAAAGAAATTGGAGAGAAACCCAAATACAGAGGATTTGAGTTCAATGAATACTTAGCGTTGAGATTGAAGAACAAAACCCATCCACCTTCAAGAGTGGAGGATGAGATTAGCATCAAACATTATGAAATCAATTGAAAAATATATGTAAGAACAGAACCAACCACAGAGAAAAATAATGTAAAAGCACTTCCTGCCATCTCTATCTGTTGCTTTTACTGTCATGAAAGCTCGAGAGAGCCCTTTAAACTCGCTTGTTGTATGGTCATTTCATTTCATCATCCTACACTATGTTCAGTCTTTATATTAGAAATGTATGAAGAGAGAAAAAAAAAAAAAACATAGTGTTTAGTGGACATAGTTCATATTTGCTTCCCTTATCTTTTTATTATTGTTCTTGTTTCTTGGAAGCTTAGGTTGATTTTGGATTACTTATGGCAGCAAGCTTCACATCATCCCTGAAAATGCCAAGTTCCAGAAACACACACTGAGAGAGAGATTCAGGTTGAGGTTGGTATCTCAATTTTTGCTCTTTTCTTGATGAAACTTGTTCATTCTTGACCCATTAGATTGGTGTGGAATAGTGTTCAAGCTTTATGTTTGAGAATTCTGATTAGCCTTAGTGATAATTTAGGCCTCTATCATGTCATTTGTTTGAGCATGTTGGACGTTTATATATGTATGATATACTGGTAGGAGCTTGTTATAGGAGCGAAACGATATTCATTATAT

mRNA sequence

CACATCACTCTGCAACTTAAGAACTCCTTCGAAAATGGCTAACACAGGCATCCCTACTGTGGACGTTTCTCTGTTCTTAAGTGAAGGTGAAAACGAAGCCAAAAAACAGGCCCTTCAAACCATAACTGATGCTTGTTCAAGCTATGGCTTCTTTCAAATTGTGAACCATGGAATCCCCGTTGAGTTTCTTAAAGAAGCTCTGCAACTGTCAAAAACATTTTTCCACTACCCTGATGAGATAAAGCTTCAATATAGCCCCAAGCCTGGGGCTCCCCTTCTTGCTGGCTTCAACAAGCAGAAGACAAATTGTGTAGACAAGAATGAATATGTCCTCATTTTCCCTCCTGGATCCAAATTCAACATCTACCCACAAGAGCCACCACAATTCAAGGAAACGCTGGAAGAGATGTTCCTGAAACTAAACGATGTGTCCTTGGTTATAGAGAGCATATTGAATGAGTGTTTGGGGCTTGCTCCTGGTTTCCTCAAACAATTCAACAATGACAGAAGCTGGGACTTCATGACTAATCTCTACTATTTCCCTGCAGCTGATGTGGGAGAAAATGGCCTCATTCACCATGAAGATGCAAACTGCATCACTTTGGTGATCCAGGATGATGCTGGAGGCCTTCAAGTCCAGAAGGACAGCGAATGGATTCCAGTCACCCCTGTTGAAGGAGCTATTGTTGTGAACGTGGGAGATATGATTCAGGTGTTGAGCAACAAGAAATTCAAGAGCGCCACACACAGAGTGGTGAGGCAAAAGGGAAAAGAGAGATATTCTTTTGCATTCTTCCGCAGCTTGCATGGAGACAAATGGGTGGAGCCATTGCCAGAGTTCACTAAAGAAATTGGAGAGAAACCCAAATACAGAGGATTTGAGTTCAATGAATACTTAGCGTTGAGATTGAAGAACAAAACCCATCCACCTTCAAGAGTGGAGGATGAGATTAGCATCAAACATTATGAAATCAATTGAAAAATATATGTAAGAACAGAACCAACCACAGAGAAAAATAATGTAAAAGCACTTCCTGCCATCTCTATCTGTTGCTTTTACTGTCATGAAAGCTCGAGAGAGCCCTTTAAACTCGCTTGTTGTATGGTCATTTCATTTCATCATCCTACACTATGTTCAGTCTTTATATTAGAAATGTATGAAGAGAGAAAAAAAAAAAAAACATAGTGTTTAGTGGACATAGTTCATATTTGCTTCCCTTATCTTTTTATTATTGTTCTTGTTTCTTGGAAGCTTAGGTTGATTTTGGATTACTTATGGCAGCAAGCTTCACATCATCCCTGAAAATGCCAAGTTCCAGAAACACACACTGAGAGAGAGATTCAGGTTGAGGTTGGTATCTCAATTTTTGCTCTTTTCTTGATGAAACTTGTTCATTCTTGACCCATTAGATTGGTGTGGAATAGTGTTCAAGCTTTATGTTTGAGAATTCTGATTAGCCTTAGTGATAATTTAGGCCTCTATCATGTCATTTGTTTGAGCATGTTGGACGTTTATATATGTATGATATACTGGTAGGAGCTTGTTATAGGAGCGAAACGATATTCATTATAT

Coding sequence (CDS)

ATGGCTAACACAGGCATCCCTACTGTGGACGTTTCTCTGTTCTTAAGTGAAGGTGAAAACGAAGCCAAAAAACAGGCCCTTCAAACCATAACTGATGCTTGTTCAAGCTATGGCTTCTTTCAAATTGTGAACCATGGAATCCCCGTTGAGTTTCTTAAAGAAGCTCTGCAACTGTCAAAAACATTTTTCCACTACCCTGATGAGATAAAGCTTCAATATAGCCCCAAGCCTGGGGCTCCCCTTCTTGCTGGCTTCAACAAGCAGAAGACAAATTGTGTAGACAAGAATGAATATGTCCTCATTTTCCCTCCTGGATCCAAATTCAACATCTACCCACAAGAGCCACCACAATTCAAGGAAACGCTGGAAGAGATGTTCCTGAAACTAAACGATGTGTCCTTGGTTATAGAGAGCATATTGAATGAGTGTTTGGGGCTTGCTCCTGGTTTCCTCAAACAATTCAACAATGACAGAAGCTGGGACTTCATGACTAATCTCTACTATTTCCCTGCAGCTGATGTGGGAGAAAATGGCCTCATTCACCATGAAGATGCAAACTGCATCACTTTGGTGATCCAGGATGATGCTGGAGGCCTTCAAGTCCAGAAGGACAGCGAATGGATTCCAGTCACCCCTGTTGAAGGAGCTATTGTTGTGAACGTGGGAGATATGATTCAGGTGTTGAGCAACAAGAAATTCAAGAGCGCCACACACAGAGTGGTGAGGCAAAAGGGAAAAGAGAGATATTCTTTTGCATTCTTCCGCAGCTTGCATGGAGACAAATGGGTGGAGCCATTGCCAGAGTTCACTAAAGAAATTGGAGAGAAACCCAAATACAGAGGATTTGAGTTCAATGAATACTTAGCGTTGAGATTGAAGAACAAAACCCATCCACCTTCAAGAGTGGAGGATGAGATTAGCATCAAACATTATGAAATCAATTGA

Protein sequence

MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPSRVEDEISIKHYEIN

Homology

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.1e-36
Identity = 92/289 (31.83%), Postives = 152/289 (52.60%), Query Frame = 0


Query: 7   PTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHYP 66
           P VD+SL       E +   L+ I DAC ++GFF++VNHG+  E L    +++K   HY 
Sbjct: 5   PVVDLSLV----NGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKD--HYK 64

Query: 67  DEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETLEEMF 126
             ++ ++     A  L     +  +   ++ + L   P S  +  P    ++++T++E  
Sbjct: 65  KTMEQRFKEMVAAKGLDDVQSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTMKEFA 124

Query: 127 LKLNDVSLVIESILNECLGLAPGFLKQ-FNNDRSWDFMTNLYYFPAADVGE--NGLIHHE 186
           ++L  ++  +  +L E LGL  G+LK+ F   +  +F T +  +P     +   GL  H 
Sbjct: 125 VELEKLAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHS 184

Query: 187 DANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVR 246
           DA  I L+ QDD   GLQ+ KD EW+ V P+  +IV+N+GD I+V++N K+KS  HRV+ 
Sbjct: 185 DAGGIILLFQDDKVSGLQLLKDGEWVDVPPMHHSIVINLGDQIEVITNGKYKSVMHRVIA 244

Query: 247 QKGKERYSFAFFRSLHGDKWVEPLPE-FTKEIGEKPKYRGFEFNEYLAL 291
           Q    R S A F +   D ++ P P    K+  + P Y  F F++Y+ L
Sbjct: 245 QSDGTRMSIASFYNPGNDSFISPAPAVLEKKTEDAPTYPKFVFDDYMKL 287

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy Swiss-Prot
Match: O48882 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 4.6e-36
Identity = 94/289 (32.53%), Postives = 149/289 (51.56%), Query Frame = 0


Query: 7   PTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHYP 66
           P VD+ L       E +   L+ I DAC ++GFF++VNHGI  E L    +++K   HY 
Sbjct: 5   PVVDMDLI----NGEERAATLEKINDACENWGFFELVNHGISTELLDTVEKMNKD--HYK 64

Query: 67  DEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETLEEMF 126
             ++ ++     A  L     +      ++ + L   P S  +  P     +++T++E  
Sbjct: 65  KTMEQRFKEMVAAKGLEAVQSEIHYLDWESTFFLRHLPSSNISEIPDLEEDYRKTMKEFA 124

Query: 127 LKLNDVSLVIESILNECLGLAPGFLKQ-FNNDRSWDFMTNLYYFPAADVGE--NGLIHHE 186
           ++L  ++  +  +L E LGL  G+LK+ F   +  +F T +  +P     +   GL  H 
Sbjct: 125 VELEKLAEKLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHT 184

Query: 187 DANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVR 246
           DA  I L+ QDD   GLQ+ KD EW+ V PV  +IV+N+GD I+V++N K+KS  HRV+ 
Sbjct: 185 DAGGIILLFQDDKVSGLQLLKDGEWMDVPPVHHSIVINLGDQIEVITNGKYKSIMHRVIA 244

Query: 247 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGE-KPKYRGFEFNEYLAL 291
           Q    R S A F +   D ++ P P   +E  E  P Y  F F++Y+ L
Sbjct: 245 QSDGTRMSIASFYNPGDDAFISPAPALLEEKSEVSPTYPKFLFDDYMKL 287

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy Swiss-Prot
Match: Q8S932 (1-aminocyclopropane-1-carboxylate oxidase OS=Diospyros kaki OX=35925 GN=DK-ACO1 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.3e-35
Identity = 92/278 (33.09%), Postives = 147/278 (52.88%), Query Frame = 0


Query: 18  GENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHYPDEIKLQYSPKP 77
           GE  A    L  I DAC ++GFF++VNHGIP E +    +++K   HY   ++ ++    
Sbjct: 14  GEERAATMGL--INDACENWGFFELVNHGIPPELMDTVERVTKA--HYKKCMEQRFKELV 73

Query: 78  GAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETLEEMFLKLNDVSLVIE 137
            +  L G   + T+   ++ Y L   P S  +  P    +++  +++   +L  ++  + 
Sbjct: 74  ASKALEGIQAEVTDMDWESTYFLRHLPQSNISEVPDLDEEYRRVMKDFAERLEKLAEYLL 133

Query: 138 SILNECLGLAPGFLKQ-FNNDRSWDFMTNLYYFPAADVGE--NGLIHHEDANCITLVIQD 197
            +L E LGL  G+LK+ F   +  +F T +  +P     +   GL  H DA  I L+ QD
Sbjct: 134 DLLCENLGLEKGYLKKAFYGTKGPNFGTKVANYPPCPKADLIKGLRAHTDAGGIILLFQD 193

Query: 198 D-AGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVRQKGKERYSFAF 257
           D   GLQ+ KD +WI V P++ +IV+N+GD ++V++N K+KS  HRVV Q    R S A 
Sbjct: 194 DKVSGLQLLKDDQWIDVPPMKHSIVINLGDQLEVITNGKYKSVLHRVVAQTDGTRMSIAS 253

Query: 258 FRSLHGDKWVEPLPEFT-KEIGEKPKYRGFEFNEYLAL 291
           F +   D  + P P    KE+ EK  Y  F F++Y+ L
Sbjct: 254 FYNPGNDAVIYPAPALVEKEVEEKEVYPKFVFDDYMKL 287

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy Swiss-Prot
Match: O04395 (Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.7e-35
Identity = 100/304 (32.89%), Postives = 157/304 (51.64%), Query Frame = 0


Query: 6   IPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHY 65
           +P VD+S          ++   +T+  A   +G FQ+VNHGIP E ++   ++ + FF  
Sbjct: 2   VPVVDLSC-------PDEELVARTVVKASEDWGVFQVVNHGIPTELIQRLQKVGREFFEL 61

Query: 66  PDEIKLQYSPKPGAPLLAGFN-------KQKTNCVDKNEYVLIFPPGS-KFNIYPQEPPQ 125
           P+  K   + + G+  + G+        K++   VD+  Y+  +PP S  +  +P+ PP 
Sbjct: 62  PEAEKRSCAREAGS--VEGYGRRIELDIKKRKGIVDQ-IYLSTWPPSSVNYRYWPKSPPD 121

Query: 126 FKETLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYY--FPAADVG 185
           ++E  EE    +  +S  I   L+E LGL    +K+ N    W  M   +Y  +P +D  
Sbjct: 122 YREVNEEYARHVKTLSEKIMEWLSEGLGLGREAIKEVNG--CWYVMNINHYPPYPHSD-S 181

Query: 186 ENGLIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKS 245
            NGL  H D N +TL+I ++  GLQV KD  WI V  +  AI+VN+GD I +LSN K+K+
Sbjct: 182 FNGLEPHTDINGLTLIITNEIPGLQVFKDDHWIEVEYIPSAIIVNIGDQIMMLSNGKYKN 241

Query: 246 ATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALR---L 297
             H+    K K R S+    S   D  V PLPE T E  + PK++   + +Y+  +   L
Sbjct: 242 VLHKTTVDKEKTRMSWPVLVSPTYDMVVGPLPELTSE-DDPPKFKPIAYKDYVHNKITFL 291

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy Swiss-Prot
Match: Q9ZSA7 (Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 6.6e-35
Identity = 95/290 (32.76%), Postives = 138/290 (47.59%), Query Frame = 0


Query: 6   IPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHY 65
           IP +D  L    G N A    +     ACSS GFFQI NHG+P E +K+ +  ++ FF  
Sbjct: 42  IPLID--LHDLHGPNRA--DIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 101

Query: 66  PDEIKLQY---SPKPGAPLLAGFNKQKTNCVDKNEYVLI--FPPGSKFNIYPQEPPQFKE 125
            +  ++++     K    L   FN  K    +  +++ +  +P     N +P  P  F+E
Sbjct: 102 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 161

Query: 126 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGE--NG 185
              E    +  + L +   ++E LGLA   +             N  Y+P     E   G
Sbjct: 162 VTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAIN--YYPRCPQPELTYG 221

Query: 186 LIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATH 245
           L  H+DAN IT+++QD+  GLQV KD +WI V PV    +VN+GD +QV+SN+K+KS  H
Sbjct: 222 LPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLH 281

Query: 246 RVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPK-YRGFEFNEY 288
           R V     ER S   F     D  + P  E   E  + P  YR F + EY
Sbjct: 282 RAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of Cp4.1LG02g10200 vs. NCBI nr
Match: XP_023524991.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650 bits (1678), Expect = 6.87e-236
Identity = 314/314 (100.00%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180
           TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 314
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

[Close]

BLAST of Cp4.1LG02g10200 vs. NCBI nr
Match: KAG6607440.1 (hypothetical protein SDJN03_00782, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037097.1 hypothetical protein SDJN02_00718 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 649 bits (1674), Expect = 2.80e-235
Identity = 313/314 (99.68%), Postives = 314/314 (100.00%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQALQTIT+ACSSYGFFQIVNHGIPVEFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITEACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180
           TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 314
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

[Close]

BLAST of Cp4.1LG02g10200 vs. NCBI nr
Match: XP_022949101.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 644 bits (1660), Expect = 3.81e-233
Identity = 310/314 (98.73%), Postives = 312/314 (99.36%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQALQTIT+ACSSYGFFQIVNHGIPVEFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITEACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYS KPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSSKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180
           TLEEMFLKLNDVSLV+ESILNECLGL PGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVVESILNECLGLPPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 314
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

[Close]

BLAST of Cp4.1LG02g10200 vs. NCBI nr
Match: XP_022998829.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima] >AAB64346.1 gibberellin 7-oxidase [Cucurbita maxima])

HSP 1 Score: 637 bits (1643), Expect = 1.49e-230
Identity = 304/314 (96.82%), Postives = 312/314 (99.36%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQA+QTIT+ACSSYGFFQIVNHGIP+EFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVL+FPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180
           TLEEMFLKL+DVSLVIESILN CLGL PGFLKQFNNDRSWDFMTNLYY+PAADVGENGLI
Sbjct: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGD+IQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKY+GFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 314
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

[Close]

BLAST of Cp4.1LG02g10200 vs. NCBI nr
Match: XP_008441091.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo])

HSP 1 Score: 548 bits (1412), Expect = 4.02e-195
Identity = 254/312 (81.41%), Postives = 286/312 (91.67%), Query Frame = 0


Query: 3   NTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTF 62
           NTGIPTVD+S+F +E ENEAKK+A +TI  ACSSYGFFQIVNHGIPVEFLKE+L++S+TF
Sbjct: 17  NTGIPTVDLSVFSTEDENEAKKKAFETIFQACSSYGFFQIVNHGIPVEFLKESLEVSRTF 76

Query: 63  FHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETL 122
           FHYPD+IKL+YSPKPGAPLLAGFNKQK NCVDKNEYVL+FPPGS FNIYPQEPPQFKE L
Sbjct: 77  FHYPDDIKLKYSPKPGAPLLAGFNKQKKNCVDKNEYVLMFPPGSNFNIYPQEPPQFKEQL 136

Query: 123 EEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLIHH 182
           EE+F KL+ V L++ESI+NE LGL P FLKQ+NNDRSWDFMT LYYF A + GENGL HH
Sbjct: 137 EELFKKLSKVCLLLESIVNEGLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGENGLTHH 196

Query: 183 EDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVR 242
           EDANCITLVIQDD GGLQV+KD EWIPV PVEGAIVVN+GD+IQVLSNKKFKSATHRVVR
Sbjct: 197 EDANCITLVIQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSATHRVVR 256

Query: 243 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPSRV 302
           QKGKER+S+AFF +LHGDKWVEPLP+FT+EIGEKPKY+GF+F +YLA+RLKNKTHPPSRV
Sbjct: 257 QKGKERFSYAFFHNLHGDKWVEPLPKFTEEIGEKPKYKGFQFKDYLAMRLKNKTHPPSRV 316

Query: 303 EDEISIKHYEIN 314
           EDEI I HYEI+
Sbjct: 317 EDEIRITHYEIS 328

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy TrEMBL
Match: A0A6J1GB31 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111452556 PE=3 SV=1)

HSP 1 Score: 644 bits (1660), Expect = 1.85e-233
Identity = 310/314 (98.73%), Postives = 312/314 (99.36%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQALQTIT+ACSSYGFFQIVNHGIPVEFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITEACSSYGFFQIVNHGIPVEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYS KPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSSKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180
           TLEEMFLKLNDVSLV+ESILNECLGL PGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI
Sbjct: 121 TLEEMFLKLNDVSLVVESILNECLGLPPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 314
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy TrEMBL
Match: O23763 (Gibberellin 7-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111493375 PE=2 SV=1)

HSP 1 Score: 637 bits (1643), Expect = 7.21e-231
Identity = 304/314 (96.82%), Postives = 312/314 (99.36%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           MANTGIPTVDVSLFLSEGENEAKKQA+QTIT+ACSSYGFFQIVNHGIP+EFLKEALQLSK
Sbjct: 1   MANTGIPTVDVSLFLSEGENEAKKQAIQTITEACSSYGFFQIVNHGIPIEFLKEALQLSK 60

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKE 120
           TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVL+FPPGSKFNIYPQEPPQFKE
Sbjct: 61  TFFHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLVFPPGSKFNIYPQEPPQFKE 120

Query: 121 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLI 180
           TLEEMFLKL+DVSLVIESILN CLGL PGFLKQFNNDRSWDFMTNLYY+PAADVGENGLI
Sbjct: 121 TLEEMFLKLSDVSLVIESILNVCLGLPPGFLKQFNNDRSWDFMTNLYYYPAADVGENGLI 180

Query: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRV 240
           HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGD+IQVLSNKKFKSATHRV
Sbjct: 181 HHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDIIQVLSNKKFKSATHRV 240

Query: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPS 300
           VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKY+GFEFNEYLALRLKNKTHPPS
Sbjct: 241 VRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYKGFEFNEYLALRLKNKTHPPS 300

Query: 301 RVEDEISIKHYEIN 314
           RVEDEISIKHYEIN
Sbjct: 301 RVEDEISIKHYEIN 314

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy TrEMBL
Match: A0A1S3B2M0 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103485283 PE=3 SV=1)

HSP 1 Score: 548 bits (1412), Expect = 1.95e-195
Identity = 254/312 (81.41%), Postives = 286/312 (91.67%), Query Frame = 0


Query: 3   NTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTF 62
           NTGIPTVD+S+F +E ENEAKK+A +TI  ACSSYGFFQIVNHGIPVEFLKE+L++S+TF
Sbjct: 17  NTGIPTVDLSVFSTEDENEAKKKAFETIFQACSSYGFFQIVNHGIPVEFLKESLEVSRTF 76

Query: 63  FHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETL 122
           FHYPD+IKL+YSPKPGAPLLAGFNKQK NCVDKNEYVL+FPPGS FNIYPQEPPQFKE L
Sbjct: 77  FHYPDDIKLKYSPKPGAPLLAGFNKQKKNCVDKNEYVLMFPPGSNFNIYPQEPPQFKEQL 136

Query: 123 EEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLIHH 182
           EE+F KL+ V L++ESI+NE LGL P FLKQ+NNDRSWDFMT LYYF A + GENGL HH
Sbjct: 137 EELFKKLSKVCLLLESIVNEGLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGENGLTHH 196

Query: 183 EDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVR 242
           EDANCITLVIQDD GGLQV+KD EWIPV PVEGAIVVN+GD+IQVLSNKKFKSATHRVVR
Sbjct: 197 EDANCITLVIQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSATHRVVR 256

Query: 243 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPSRV 302
           QKGKER+S+AFF +LHGDKWVEPLP+FT+EIGEKPKY+GF+F +YLA+RLKNKTHPPSRV
Sbjct: 257 QKGKERFSYAFFHNLHGDKWVEPLPKFTEEIGEKPKYKGFQFKDYLAMRLKNKTHPPSRV 316

Query: 303 EDEISIKHYEIN 314
           EDEI I HYEI+
Sbjct: 317 EDEIRITHYEIS 328

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy TrEMBL
Match: A0A6J1C816 (flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111009081 PE=3 SV=1)

HSP 1 Score: 537 bits (1383), Expect = 2.73e-191
Identity = 247/309 (79.94%), Postives = 281/309 (90.94%), Query Frame = 0


Query: 6   IPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHY 65
           IPTVD+SLF  EG++E KK+A +TIT+ACSSYGFFQIVNHG+P+EFL  AL+LS+TFF Y
Sbjct: 3   IPTVDLSLFCREGDDEGKKKAFETITEACSSYGFFQIVNHGVPIEFLSGALELSRTFFQY 62

Query: 66  PDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETLEEM 125
           PD++KL+YS KPGAPLLAGFNKQK  CVDKNEYVL+FPPGS +NIYPQEPPQFKE LEEM
Sbjct: 63  PDDVKLKYSSKPGAPLLAGFNKQKDKCVDKNEYVLVFPPGSAYNIYPQEPPQFKEVLEEM 122

Query: 126 FLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLIHHEDA 185
           FLKL+ V L++ESI+N+CLGL PGFLKQ+N++R+WDFMTNLYYFPAA   ENGL HHED 
Sbjct: 123 FLKLSKVCLLLESIVNDCLGLPPGFLKQYNDNRTWDFMTNLYYFPAAGDNENGLTHHEDG 182

Query: 186 NCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVRQKG 245
           NCITLVIQD AGGLQV+K+ EWIPVTPV+GAIVVN+GD+IQVLSNKKFKSATHRVVRQKG
Sbjct: 183 NCITLVIQDAAGGLQVRKNGEWIPVTPVQGAIVVNIGDVIQVLSNKKFKSATHRVVRQKG 242

Query: 246 KERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPSRVEDE 305
           KER+S+AFF +LHGDKWVEPLP FTK+IGEKPKYRGFEF EY ALRLKNKTHPPSRVEDE
Sbjct: 243 KERFSYAFFHNLHGDKWVEPLPHFTKQIGEKPKYRGFEFKEYQALRLKNKTHPPSRVEDE 302

Query: 306 ISIKHYEIN 314
           I I HYEI+
Sbjct: 303 IHITHYEIS 311

[Close]

BLAST of Cp4.1LG02g10200 vs. ExPASy TrEMBL
Match: S0BE22 (GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox1 PE=2 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 3.58e-191
Identity = 247/312 (79.17%), Postives = 281/312 (90.06%), Query Frame = 0


Query: 3   NTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTF 62
           +TGIPTVD+S+F ++ E EAKK+A +TI  ACSSYGFFQIVNHG+P+EFL+EAL+LS+TF
Sbjct: 17  DTGIPTVDLSVFSAQNETEAKKKAFETIYQACSSYGFFQIVNHGVPIEFLEEALELSRTF 76

Query: 63  FHYPDEIKLQYSPKPGAPLLAGFNKQKTNCVDKNEYVLIFPPGSKFNIYPQEPPQFKETL 122
           FHYPD+IKL+YS KPGAPLLAGFNKQK NCVDKNEYVL+FPPGS +NIYPQEPPQFKE L
Sbjct: 77  FHYPDDIKLKYSSKPGAPLLAGFNKQKKNCVDKNEYVLVFPPGSNYNIYPQEPPQFKELL 136

Query: 123 EEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGENGLIHH 182
           EEMF KL+ V L++ESI+NE LGL P FLKQ+NNDRSWDFMT LYYF A + GENGL HH
Sbjct: 137 EEMFKKLSKVCLLLESIVNESLGLPPDFLKQYNNDRSWDFMTTLYYFSATEEGENGLTHH 196

Query: 183 EDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATHRVVR 242
           ED NCITLV QDD GGLQV+KD EWIPV PVEGAIVVN+GD+IQVLSNKKFKSATHRVVR
Sbjct: 197 EDGNCITLVFQDDTGGLQVRKDGEWIPVVPVEGAIVVNIGDVIQVLSNKKFKSATHRVVR 256

Query: 243 QKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNKTHPPSRV 302
           QKGKER+S+AFF +LHGDKWVEPLP+FT+EIGEKPKY+GF+F +Y ALRLKNKTHPPSRV
Sbjct: 257 QKGKERFSYAFFHNLHGDKWVEPLPQFTEEIGEKPKYKGFQFKDYQALRLKNKTHPPSRV 316

Query: 303 EDEISIKHYEIN 314
           EDEI I HYEI+
Sbjct: 317 EDEIRITHYEIS 328

[Close]

BLAST of Cp4.1LG02g10200 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 4.7e-36
Identity = 95/290 (32.76%), Postives = 138/290 (47.59%), Query Frame = 0


Query: 6   IPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHY 65
           IP +D  L    G N A    +     ACSS GFFQI NHG+P E +K+ +  ++ FF  
Sbjct: 42  IPLID--LHDLHGPNRA--DIINQFAHACSSCGFFQIKNHGVPEETIKKMMNAAREFFRQ 101

Query: 66  PDEIKLQY---SPKPGAPLLAGFNKQKTNCVDKNEYVLI--FPPGSKFNIYPQEPPQFKE 125
            +  ++++     K    L   FN  K    +  +++ +  +P     N +P  P  F+E
Sbjct: 102 SESERVKHYSADTKKTTRLSTSFNVSKEKVSNWRDFLRLHCYPIEDFINEWPSTPISFRE 161

Query: 126 TLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGE--NG 185
              E    +  + L +   ++E LGLA   +             N  Y+P     E   G
Sbjct: 162 VTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHGQHMAIN--YYPRCPQPELTYG 221

Query: 186 LIHHEDANCITLVIQDDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKSATH 245
           L  H+DAN IT+++QD+  GLQV KD +WI V PV    +VN+GD +QV+SN+K+KS  H
Sbjct: 222 LPGHKDANLITVLLQDEVSGLQVFKDGKWIAVNPVPNTFIVNLGDQMQVISNEKYKSVLH 281

Query: 246 RVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPK-YRGFEFNEY 288
           R V     ER S   F     D  + P  E   E  + P  YR F + EY
Sbjct: 282 RAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSPAIYRNFTYAEY 325

[Close]

BLAST of Cp4.1LG02g10200 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 6.1e-36
Identity = 107/315 (33.97%), Postives = 153/315 (48.57%), Query Frame = 0


Query: 4   TGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFF 63
           T IP +D+    SE E  +    +  I++AC  +GFFQ+VNHG+  E +  A +  + FF
Sbjct: 60  TNIPIIDLEGLFSE-EGLSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFF 119

Query: 64  HYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNEYVL--IFPPGSK-FNIYPQEPPQF 123
           H P   K  YS  P      G     +K   +D ++Y    + P   K FN +P  PP  
Sbjct: 120 HMPVNAKETYSNSPRTYEGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTI 179

Query: 124 KETLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVGE-- 183
           +E ++E   +L  +S  I  +L+  LGL     ++     +      + Y+P     E  
Sbjct: 180 REVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFGGENIGACLRVNYYPKCPRPELA 239

Query: 184 NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKS 243
            GL  H D   +T+++ DD   GLQV+KD  WI V P   A +VN+GD IQ+LSN  +KS
Sbjct: 240 LGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKS 299

Query: 244 ATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNK 303
             HRV+    KER S AFF +   D  ++PL E        P Y    F++Y   RL  +
Sbjct: 300 VEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELV-STHNPPLYPPMTFDQY---RLFIR 359

Query: 304 THPP---SRVEDEIS 308
           T  P   S VE  IS
Sbjct: 360 TQGPQGKSHVESHIS 369

[Close]

BLAST of Cp4.1LG02g10200 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.3e-35
Identity = 104/318 (32.70%), Postives = 156/318 (49.06%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           +A+  IP +D+    S  E++ K+     I++AC  +GFFQ++NHG+  E +  A +  K
Sbjct: 90  VADINIPIIDLDSLFSGNEDDKKR-----ISEACREWGFFQVINHGVKPELMDAARETWK 149

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNEYVLI--FPPGSK-FNIYPQEP 120
           +FF+ P E K  YS  P      G     +K   +D N+Y  +   P   K FN +P  P
Sbjct: 150 SFFNLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLP 209

Query: 121 PQFKETLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVG 180
              +E  +E   +L  +   + +IL+  LGL    L++            + Y+P     
Sbjct: 210 SNIREMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQP 269

Query: 181 E--NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKK 240
           E   GL  H D   +T+++ DD   GLQV+    WI V P+  A +VN+GD IQ+LSN K
Sbjct: 270 ELALGLSPHSDPGGMTILLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSK 329

Query: 241 FKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRL 300
           +KS  HRV+    KER S AFF +   D  ++P+ +        P Y    F++Y   RL
Sbjct: 330 YKSVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTST-MPPLYPPMTFDQY---RL 389

Query: 301 KNKTHPP---SRVEDEIS 308
             +T  P   S VE  IS
Sbjct: 390 FIRTQGPRGKSHVESHIS 398

[Close]

BLAST of Cp4.1LG02g10200 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 2.0e-34
Identity = 95/295 (32.20%), Postives = 146/295 (49.49%), Query Frame = 0


Query: 1   MANTGIPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSK 60
           +A+  IP +D+    S  E++ K+     I++AC  +GFFQ++NHG+  E +  A +  K
Sbjct: 90  VADINIPIIDLDSLFSGNEDDKKR-----ISEACREWGFFQVINHGVKPELMDAARETWK 149

Query: 61  TFFHYPDEIKLQYSPKPGAPLLAG--FNKQKTNCVDKNEYVLI--FPPGSK-FNIYPQEP 120
           +FF+ P E K  YS  P      G     +K   +D N+Y  +   P   K FN +P  P
Sbjct: 150 SFFNLPVEAKEVYSNSPRTYEGYGSRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLP 209

Query: 121 PQFKETLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVG 180
              +E  +E   +L  +   + +IL+  LGL    L++            + Y+P     
Sbjct: 210 SNIREMNDEYGKELVKLGGRLMTILSSNLGLRAEQLQEAFGGEDVGACLRVNYYPKCPQP 269

Query: 181 E--NGLIHHEDANCITLVIQDD-AGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKK 240
           E   GL  H D   +T+++ DD   GLQV+    WI V P+  A +VN+GD IQ+LSN K
Sbjct: 270 ELALGLSPHSDPGGMTILLPDDQVVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSK 329

Query: 241 FKSATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEY 288
           +KS  HRV+    KER S AFF +   D  ++P+ +        P Y    F++Y
Sbjct: 330 YKSVEHRVIVNSEKERVSLAFFYNPKSDIPIQPMQQLVTST-MPPLYPPMTFDQY 378

[Close]

BLAST of Cp4.1LG02g10200 vs. TAIR 10
Match: AT3G21420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 7.5e-34
Identity = 90/300 (30.00%), Postives = 156/300 (52.00%), Query Frame = 0


Query: 6   IPTVDVSLFLSEGENEAKKQALQTITDACSSYGFFQIVNHGIPVEFLKEALQLSKTFFHY 65
           IP +D+S  LS+ +N+     +  ++ AC  +GFFQ++NHGI VE +++  +++  FF  
Sbjct: 55  IPVIDLSK-LSKPDNDDFFFEILKLSQACEDWGFFQVINHGIEVEVVEDIEEVASEFFDM 114

Query: 66  PDEIKLQYSPKPGAPLLAGFNKQKTNCVDK-----NEYVL-IFPPGSKF-NIYPQEPPQF 125
           P E K +Y  +PG   + G+ +      D+     N + L + PP  +   ++P +P +F
Sbjct: 115 PLEEKKKYPMEPGT--VQGYGQAFIFSEDQKLDWCNMFALGVHPPQIRNPKLWPSKPARF 174

Query: 126 KETLEEMFLKLNDVSLVIESILNECLGLAPGFLKQFNNDRSWDFMTNLYYFPAADVG-EN 185
            E+LE    ++ ++   +   +   LGL     ++   +       N YY P +      
Sbjct: 175 SESLEGYSKEIRELCKRLLKYIAISLGLKEERFEEMFGEAVQAVRMN-YYPPCSSPDLVL 234

Query: 186 GLIHHEDANCITLVIQ--DDAGGLQVQKDSEWIPVTPVEGAIVVNVGDMIQVLSNKKFKS 245
           GL  H D + +T++ Q  +   GLQ+ KD+ W+PV P+  A+V+N+GD I+VLSN K+KS
Sbjct: 235 GLSPHSDGSALTVLQQSKNSCVGLQILKDNTWVPVKPLPNALVINIGDTIEVLSNGKYKS 294

Query: 246 ATHRVVRQKGKERYSFAFFRSLHGDKWVEPLPEFTKEIGEKPKYRGFEFNEYLALRLKNK 296
             HR V  + KER +   F + + +  +EP+ E   +     KYR +   +Y    + NK
Sbjct: 295 VEHRAVTNREKERLTIVTFYAPNYEVEIEPMSELVDDETNPCKYRSYNHGDYSYHYVSNK 350

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g10200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q00985	2.1e-36	31.83	1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1	[more]
O48882	4.6e-36	32.53	1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 P...	[more]
Q8S932	1.3e-35	33.09	1-aminocyclopropane-1-carboxylate oxidase OS=Diospyros kaki OX=35925 GN=DK-ACO1 ...	[more]
O04395	1.7e-35	32.89	Flavonol synthase/flavanone 3-hydroxylase (Fragment) OS=Matthiola incana OX=3724...	[more]
Q9ZSA7	6.6e-35	32.76	Protein DMR6-LIKE OXYGENASE 2 OS=Arabidopsis thaliana OX=3702 GN=DLO2 PE=2 SV=1	[more]

BLAST of Cp4.1LG02g10200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023524991.1	6.87e-236	100.00	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6607440.1	2.80e-235	99.68	hypothetical protein SDJN03_00782, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022949101.1	3.81e-233	98.73	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita moschata]	[more]
XP_022998829.1	1.49e-230	96.82	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita maxima] >AAB64346.1 gi...	[more]
XP_008441091.1	4.02e-195	81.41	PREDICTED: flavonol synthase/flavanone 3-hydroxylase-like [Cucumis melo]	[more]

BLAST of Cp4.1LG02g10200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GB31	1.85e-233	98.73	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
O23763	7.21e-231	96.82	Gibberellin 7-oxidase OS=Cucurbita maxima OX=3661 GN=LOC111493375 PE=2 SV=1	[more]
A0A1S3B2M0	1.95e-195	81.41	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1C816	2.73e-191	79.94	flavonol synthase/flavanone 3-hydroxylase-like OS=Momordica charantia OX=3673 GN...	[more]
S0BE22	3.58e-191	79.17	GA 7-oxidase OS=Cucumis sativus OX=3659 GN=GA7ox1 PE=2 SV=1	[more]

BLAST of Cp4.1LG02g10200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10490.1	4.7e-36	32.76	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G05600.1	6.1e-36	33.97	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.1	2.3e-35	32.70	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.2	2.0e-34	32.20	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G21420.1	7.5e-34	30.00	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 164..255 e-value: 1.1E-21 score: 77.1
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..298 e-value: 2.0E-81 score: 275.8
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 6..87 e-value: 1.9E-18 score: 67.3
None	No IPR available	PANTHER	PTHR10209:SF732	FLAVONOL SYNTHASE/FLAVANONE 3-HYDROXYLASE-LIKE	coord: 1..313
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 1..313
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 4..295
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 159..257 score: 11.852057

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g10200.1	Cp4.1LG02g10200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding