Cp4.1LG02g08540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG02g08540 vs. ExPASy Swiss-Prot
Match: O23144 (Proton pump-interactor 1 OS=Arabidopsis thaliana OX=3702 GN=PPI1 PE=1 SV=2)

HSP 1 Score: 130.2 bits (326), Expect = 2.1e-28
Identity = 142/577 (24.61%), Postives = 278/577 (48.18%), Query Frame = 0

Query: 1017 FLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGR 1076
            +LV+   + D  I+ ++  A  E+++  K R  +  +++  RA    L D L+   SE +
Sbjct: 80   YLVKYRSYADPKIKAKLDLADKELEKLNKARTGVLDKLRAKRAERSELFDLLDPLKSERK 139

Query: 1077 AARDLLRFKRQEIDSVQSVITKVKN-----------AMSIGDIDGRVRSIEHIIEHETLP 1136
                +   KR+E++ +Q  + K+++             S  +++  + S ++ I+HE++P
Sbjct: 140  GFNTMFDEKRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMIYSYQYRIQHESIP 199

Query: 1137 LKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLL-------RKEMD 1196
            L EEKQ+++EI+ L+  R+++ +    + ++++++ QKD  + ++KL+       +KE  
Sbjct: 200  LTEEKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDGVKKERQ 259

Query: 1197 LLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNK 1256
             +   + +    +KA K        ++  L+++ K   + R +AY+N+   R+Q  + N 
Sbjct: 260  AISARINELSEKLKATKD-------EITVLENELKTVSEKRDKAYSNIHDLRRQRDETNS 319

Query: 1257 YYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRR 1316
             Y++ R  + +A ++A   ++  L+  +  +VE  + LW +   FRE+Y K  ++S   R
Sbjct: 320  EYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSR 379

Query: 1317 LKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEV 1376
              + DGR   P+E+P          P    S +T S+T     ++P    +   KP    
Sbjct: 380  QLSRDGRMRNPDEKPLI-------APEAAPSKATPSET----EVVP----KAKAKP---- 439

Query: 1377 VKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEE----LAAKAEE 1436
             + K +P    KP   VA            + E+    V++K   ++++       K ++
Sbjct: 440  -QPKEEPVSAPKPDATVA-----------QNTEKAKDAVKVKNVADDDDDEVYGLGKPQK 499

Query: 1437 LRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAK 1496
              K  +A   KE  K EE  KAK+A+ERKK+ AEKA A+A  +A+KEAE++EK   K  +
Sbjct: 500  EEKPVDAATAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEK---KEQE 559

Query: 1497 KKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSK 1556
            KKA+K     TE        E+  + E++ +E+     ++    ++  K    +     +
Sbjct: 560  KKAKKKTGGNTET-------ETEEVPEASEEEIEAPVQEEKPQKEKVFKEKPIRNRTRGR 607

Query: 1557 TKSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFVG 1572
               TIP  +  R K+    W++     L+V ++  +G
Sbjct: 620  GPETIPRAILKR-KKSTNYWVYAAPAALVVLLLLVLG 607

BLAST of Cp4.1LG02g08540 vs. ExPASy Swiss-Prot
Match: Q6ZBF6 (Proton pump-interactor BIP131 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP131 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 8.5e-22
Identity = 141/569 (24.78%), Postives = 256/569 (44.99%), Query Frame = 0

Query: 1017 FLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGR 1076
            F VR+  + D +++ +++ A  E  +  + R  I   ++  R+    +  +L+   +E +
Sbjct: 78   FFVRICSYEDPSLKAKLEQADKECQKKIQARSHIFEALRTKRSERSNIISELKPLAAENK 137

Query: 1077 AARDLLRFKRQEIDSVQSVITKVK---NAM---------SIGDIDGRVRSIEHIIEHETL 1136
               +++  K +EI+ +Q  + K +   NAM         SI ++D  ++S+   I HE++
Sbjct: 138  QYNEVVSGKLKEIEPLQKSLGKFRSENNAMRAQGAGLCSSIEELDQLIKSLNDRISHESI 197

Query: 1137 PLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNV 1196
             L EEK+L++EIKQL   R ++     K+ ++Q  + ++    +++K +   +D ++ + 
Sbjct: 198  SLDEEKRLVKEIKQLNGTRSKVIENAAKRAKMQDTVVERGTIHDQVKQIGVGIDEVKRDR 257

Query: 1197 LKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYR 1256
                  IK  +   +    ++  LQ    AA   + +A+  L   RK     N  + +YR
Sbjct: 258  QAVRDKIKVLEDQIHAVDGEIAALQDDLTAATARKDKAFEALNELRKTRDLNNTSFHQYR 317

Query: 1257 DDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDG 1316
                   +++  G+VE +Q+   N+VE  M  W ++  FRE+Y K  + S   R  + DG
Sbjct: 318  TISNSVRDLSARGEVEAVQQLCQNEVEKFMAQWCSSKSFREDYEKRILVSLNSRQLSRDG 377

Query: 1317 RSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQ 1376
            R   P+E+P  L   V   P  +   + + K        PA+  ++   P  +V  T   
Sbjct: 378  RMRNPDEKPIVLETQV--APPAEQEPAPLKK--------PAKQAKEAPAPRADV--TPKD 437

Query: 1377 PTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKL 1436
              + K P                    +  QP++I    +  +     E+ + K +  KL
Sbjct: 438  EIRAKAPAKAA----------------KAKQPLDIDDIPDVHDDEPPKEKTKPKVDEAKL 497

Query: 1437 KEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAE 1496
            KE  + EE  K K ALERKK+ AEK   +A  +A KEAE++ K +EK+A+K++     AE
Sbjct: 498  KEMKRQEEIEKNKLALERKKKQAEKQAMKAAARAEKEAEKKLKEKEKKARKRSATAGGAE 557

Query: 1497 TEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIPPPLR 1556
            +E              ES  K       ++  AA    K +    +  +KTK+ +P  + 
Sbjct: 558  SEEA-----------AESDAKSDEAEAQEEEPAAPVTIKKNARHRSTVTKTKTPLPKAVL 606

Query: 1557 NRSKRGMQPWMW-VLLTTLIVFVVFFVGA 1573
             R K+    W W   +  L   +V  +GA
Sbjct: 618  KR-KKSQAFWSWGAPMAALAAALVALLGA 606

BLAST of Cp4.1LG02g08540 vs. ExPASy Swiss-Prot
Match: Q762B4 (Proton pump-interactor BIP103 OS=Oryza sativa subsp. japonica OX=39947 GN=BIP103 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 4.2e-21
Identity = 127/558 (22.76%), Postives = 261/558 (46.77%), Query Frame = 0

Query: 1017 FLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGR 1076
            F V++    D  ++ +I  A+ +  +  + R  I   I+  +     +  +L+   +E +
Sbjct: 81   FFVKIRLLEDPKLKMKIDQAEKDFQKKIQARSQIFEAIKAKKNERFGIISELKPLAAENK 140

Query: 1077 AARDLLRFKRQEIDSVQSVITKVKN------------AMSIGDIDGRVRSIEHIIEHETL 1136
               + +  K + I+ +++ + K ++              SI +++  ++ +   I HE++
Sbjct: 141  QYNEAVSEKLKAIEPLRNRLGKFRDENNAIRAQGAGICSSIEELEKSIKRLNDRISHESI 200

Query: 1137 PLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNV 1196
            PL EEK+LI++I++L++ R ++ ST+  + ++Q  + ++D  ++++K++ + +D     V
Sbjct: 201  PLDEEKRLIKQIRELEKTRPKVISTSANRAQIQDTVVERDAIQDQVKIIGEGID----GV 260

Query: 1197 LKAESVIKAAKKICNDE----SLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYY 1256
             K    +++  K+  DE     +++  LQ    AA+  + +A+ +L   R      N  +
Sbjct: 261  KKERQAVRSKIKVLEDELKAIDMEMGSLQEDLTAANARKDKAHESLVQLRHARDAYNASF 320

Query: 1257 WKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLK 1316
             + R  + +A ++A+  ++ ++Q     QV+  +  W  +  FRE+Y K  + S   R  
Sbjct: 321  HQNRQLLSKARDLASRSELAQVQELYKTQVDKFVAEWCNSKAFREDYEKRILSSLNSRQL 380

Query: 1317 TLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEV-V 1376
            + DGR   P+E+P  +       P     +         ++ +PA+  ++   P  EV  
Sbjct: 381  SRDGRMRNPDEKPIFIETEAAAPPVEQEPI---------QSKMPAKQAKEAPAPQAEVSP 440

Query: 1377 KTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKE 1436
            K +++     KP+   + ++   +   E+  +E P+P E+                    
Sbjct: 441  KDESRVKAIAKPSKAKSSLDADDDYEAESP-KEKPKPKEV-------------------- 500

Query: 1437 EAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARK 1496
            +  KLKE  + EE  K + ALERKK+ AEK  A+A  +A+KEAE++ K  E RA+++A  
Sbjct: 501  DVAKLKEIKRQEEMEKNRLALERKKKLAEKQAAKAAARAQKEAEKKLKREEMRARRRA-- 560

Query: 1497 MAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTI 1556
              AA+TEA    + +  G       +  +E+     +A     +    +Y+++  TKS  
Sbjct: 561  -GAADTEASTESDNRSDG-----AAEAQAEDDSAPASAPVMREQRESVRYSRNVVTKSKA 596

BLAST of Cp4.1LG02g08540 vs. ExPASy Swiss-Prot
Match: B3H4K7 (Proton pump-interactor 2 OS=Arabidopsis thaliana OX=3702 GN=PPI2 PE=2 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 4.7e-12
Identity = 136/575 (23.65%), Postives = 253/575 (44.00%), Query Frame = 0

Query: 1013 KPLNF-LVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAA 1072
            KP +F  V+ P + D  I+ +I  A  E+++  KDR  +    +  +A    L   +++ 
Sbjct: 65   KPFSFYFVKQPAYDDPEIKAKIDEAGHEINRYNKDRIVVSNAQESEKAEILSLFGQMKSL 124

Query: 1073 VSEGRAARDLLRFKRQEIDSVQSVI------TKVKNAMSIGDIDGRVRSIEHIIEHETLP 1132
            VS+    R ++  K+ E D++   +      T  +   S  ++D  +    + IEH ++ 
Sbjct: 125  VSKSEGYRVVIEEKKMEFDALHESLRNLRCSTSDQLCFSKEELDHLIYIAHYQIEHGSIG 184

Query: 1133 LKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVL 1192
             +EE  +++E ++   +     S   K+  + +      +  E    ++KE+D +   + 
Sbjct: 185  FEEEDWVLKETEKADGIVLSEDSLAEKEASINRVKSMAVELNE----VKKELDAITWKI- 244

Query: 1193 KAESVIKAAKKICNDESLKLDELQSKFKAAD-------KIRQEAYANLQSTRKQLYDKNK 1252
                         N  S K+ + Q+  +  D       + R  +Y  ++  R Q      
Sbjct: 245  -------------NHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKA 304

Query: 1253 YYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRR 1312
             +++    +++A E+A SG+V  L+ F+ ++ +  M  WN +  FR++Y+K    S   R
Sbjct: 305  AFYQSLPVMRKARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCER 364

Query: 1313 LKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEV 1372
                DGR   P        Q+V E+         V +T         E+   N      V
Sbjct: 365  QLNQDGRIKDPEV------QVVWEKKVPVKGGEKVHET-------NREDSSSNSSQYGSV 424

Query: 1373 VKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKK 1432
            +  K +    KK       M+  R+ + E+DV +    V  K  +EEEE+          
Sbjct: 425  ITDKRKKETRKK------AMDFNRSSAEESDVTDLEFSVYEKPKKEEEEV---------D 484

Query: 1433 EEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKAR 1492
            EE   LKE+ + E+  KA+ A+ERK++  EKA A+A  +A+KEAE++ K  EK+AKKKA 
Sbjct: 485  EET--LKEREREEQLEKARLAMERKRKLQEKAAAKAAIRAQKEAEKKLKECEKKAKKKAA 544

Query: 1493 KMAAAETEAGNGLEEKESGIITESTPK-EVSENTGKQGTAAKRP--PKASQSQYTKHSKT 1552
              +++ +E+ +  E      +T+   K      +GK+    +R   PK    +Y    + 
Sbjct: 545  ANSSSPSESDHSQE------VTKDLEKVRTLAVSGKEKHQKERSLFPKQRSFRYKHRGRG 584

Query: 1553 KSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFV 1571
               +P  + NR ++  + W+W L +  +   +F V
Sbjct: 605  TEALPKAILNR-RKAHKYWVWGLSSAALAVALFLV 584

BLAST of Cp4.1LG02g08540 vs. ExPASy Swiss-Prot
Match: P0DKC1 (Proton pump-interactor 3A OS=Arabidopsis thaliana OX=3702 GN=PPI3A PE=3 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 1.9e-05
Identity = 150/638 (23.51%), Postives = 263/638 (41.22%), Query Frame = 0

Query: 967  SQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNF-LVRVPKFG 1026
            S T+S+ T +E E    S  +  S ++ E              +G KPL+F +V+ P + 
Sbjct: 30   SLTNSEVTTDEEEDTIFSGGDSSSGLAAEED-----------SSGDKPLSFYIVKQPVYD 89

Query: 1027 DVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARDLLRFK 1086
            +  I+ +I  A  E+ +  + R  +    +   A    L   +E+ V +    R ++  K
Sbjct: 90   NPEIKAKIDEANQEIFRCNELRINVLSAKKSELAEVSSLYTQMESLVPQSEGYRMVIEEK 149

Query: 1087 RQEIDSVQSVITKVKNAMS------IGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQL 1146
            ++E D++   +  ++   S        ++D      ++ IE+ ++ L+EE  +++E    
Sbjct: 150  KKEFDTLLEALRNLRCTTSDQLCFTKEELDHLSYIAQYQIEYGSIGLEEEDWMLKE---- 209

Query: 1147 KQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICN 1206
                      T K D +   L +K+    R+K +  E++       + ++ + A     N
Sbjct: 210  ----------TEKPDGI--ILSEKEASINRVKSMALELN-------EVKNELDAITWKIN 269

Query: 1207 DESLKLDELQSKFKAAD-------KIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANE 1266
            D S KL + Q+  +  D       + R   YA ++  R Q    N  +++    + +A E
Sbjct: 270  DLSDKLWKSQNNIRVLDLEKAHILEKRDRFYARIKMLRIQRDKGNAAFFQSLSVMCKAKE 329

Query: 1267 IATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEE 1326
            +A SG+V  L+ F+ ++V+  M LWN +  FRE+Y++    S   R    DGR       
Sbjct: 330  LAASGNVRELEVFASSEVDRFMTLWNDDKAFREDYVRRISHSLCERELNEDGR------- 389

Query: 1327 PHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPT 1386
                                               ++D D   +++   K  P K  K +
Sbjct: 390  -----------------------------------IKDAD---LQIFWEKKVPVKTIKRS 449

Query: 1387 TVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAI---KLKEQHK 1446
              V  MN  R  S  N  E+    +  KR +E +      E+ +KKEE I    LKE+ +
Sbjct: 450  EKVHKMN--REDSSSNSSED-GNVITDKRKKETKSDVIVYEKPKKKEEEIDEEALKERKR 509

Query: 1447 LEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKL-----------REKRAKKKAR 1506
             E+  KA+  +ERK++  EKA A+A  +A+KEAE++ K             EK+AKKKA 
Sbjct: 510  EEQLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFFNECEKKAKKKA- 569

Query: 1507 KMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP--PKASQSQYTKHSKTK 1566
                    A N     ES  +           +GK+    +R   PK    +Y       
Sbjct: 570  --------AANSTSPSESDQVINDEKVRTLAVSGKEKHQKERLLFPKQRSFRYKHRGSGT 575

Query: 1567 STIPPPLRNRSKRGMQPWMWVL----LTTLIVFVVFFV 1571
              +P  + NR ++  + W+W L    L   +V VV  +
Sbjct: 630  EALPKAILNR-RKARRYWVWGLSSAALAVSLVLVVLLL 575

BLAST of Cp4.1LG02g08540 vs. NCBI nr
Match: XP_023525592.1 (uncharacterized protein LOC111789163 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2900 bits (7518), Expect = 0.0
Identity = 1561/1561 (100.00%), Postives = 1561/1561 (100.00%), Query Frame = 0

Query: 22   MSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDAATSDDHVVSDLNGQLECSHNV 81
            MSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDAATSDDHVVSDLNGQLECSHNV
Sbjct: 1    MSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDAATSDDHVVSDLNGQLECSHNV 60

Query: 82   DTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLKVSDAIQDETRVVDVLQSSDA 141
            DTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLKVSDAIQDETRVVDVLQSSDA
Sbjct: 61   DTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLKVSDAIQDETRVVDVLQSSDA 120

Query: 142  KVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGVDAPQEDENVQIQEENQIISTS 201
            KVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGVDAPQEDENVQIQEENQIISTS
Sbjct: 121  KVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGVDAPQEDENVQIQEENQIISTS 180

Query: 202  ESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSNTKANRGIDIEAESSQNAEGI 261
            ESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSNTKANRGIDIEAESSQNAEGI
Sbjct: 181  ESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSNTKANRGIDIEAESSQNAEGI 240

Query: 262  QFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEENGRVKAFNLTDTAANLRGKI 321
            QFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEENGRVKAFNLTDTAANLRGKI
Sbjct: 241  QFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEENGRVKAFNLTDTAANLRGKI 300

Query: 322  KLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVESSQKGDDILNDEENGRVKAFN 381
            KLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVESSQKGDDILNDEENGRVKAFN
Sbjct: 301  KLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVESSQKGDDILNDEENGRVKAFN 360

Query: 382  LSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGEGEDIKLNGQNEIVDAVKSSA 441
            LSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGEGEDIKLNGQNEIVDAVKSSA
Sbjct: 361  LSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGEGEDIKLNGQNEIVDAVKSSA 420

Query: 442  TLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSFEREEGIRESQDANVEAADCHS 501
            TLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSFEREEGIRESQDANVEAADCHS
Sbjct: 421  TLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSFEREEGIRESQDANVEAADCHS 480

Query: 502  GKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHPDGSVEDVKGECKLGVALNEEN 561
            GKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHPDGSVEDVKGECKLGVALNEEN
Sbjct: 481  GKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHPDGSVEDVKGECKLGVALNEEN 540

Query: 562  SERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENKVDMEQHLAAAPSPLVNSEDVN 621
            SERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENKVDMEQHLAAAPSPLVNSEDVN
Sbjct: 541  SERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENKVDMEQHLAAAPSPLVNSEDVN 600

Query: 622  GSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETLSSSVDPDIDSVETHKLTHTML 681
            GSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETLSSSVDPDIDSVETHKLTHTML
Sbjct: 601  GSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETLSSSVDPDIDSVETHKLTHTML 660

Query: 682  INDPKVELNEITVNEQEVNHVLELEETSETASHPKVDECDRVEVLEGTVSGDEMSIALDE 741
            INDPKVELNEITVNEQEVNHVLELEETSETASHPKVDECDRVEVLEGTVSGDEMSIALDE
Sbjct: 661  INDPKVELNEITVNEQEVNHVLELEETSETASHPKVDECDRVEVLEGTVSGDEMSIALDE 720

Query: 742  SRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSVEIREMSCTHGLDDPVLRPDL 801
            SRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSVEIREMSCTHGLDDPVLRPDL
Sbjct: 721  SRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSVEIREMSCTHGLDDPVLRPDL 780

Query: 802  EAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFETKSESGDIEEENQSTFPCNDM 861
            EAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFETKSESGDIEEENQSTFPCNDM
Sbjct: 781  EAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFETKSESGDIEEENQSTFPCNDM 840

Query: 862  RSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVETDSEFRDNERSSSPTANEKSG 921
            RSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVETDSEFRDNERSSSPTANEKSG
Sbjct: 841  RSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVETDSEFRDNERSSSPTANEKSG 900

Query: 922  ENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLNPMSDIASQTDSKPTKEETEVV 981
            ENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLNPMSDIASQTDSKPTKEETEVV
Sbjct: 901  ENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLNPMSDIASQTDSKPTKEETEVV 960

Query: 982  HESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVD 1041
            HESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVD
Sbjct: 961  HESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVD 1020

Query: 1042 QTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARDLLRFKRQEIDSVQSVITKVKN 1101
            QTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARDLLRFKRQEIDSVQSVITKVKN
Sbjct: 1021 QTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARDLLRFKRQEIDSVQSVITKVKN 1080

Query: 1102 AMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALD 1161
            AMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALD
Sbjct: 1081 AMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALD 1140

Query: 1162 QKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQE 1221
            QKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQE
Sbjct: 1141 QKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQE 1200

Query: 1222 AYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNA 1281
            AYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNA
Sbjct: 1201 AYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNA 1260

Query: 1282 EFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPEN 1341
            EFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPEN
Sbjct: 1261 EFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPEN 1320

Query: 1342 LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKR 1401
            LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKR
Sbjct: 1321 LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKR 1380

Query: 1402 TREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKE 1461
            TREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKE
Sbjct: 1381 TREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKE 1440

Query: 1462 AEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP 1521
            AEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP
Sbjct: 1441 AEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP 1500

Query: 1522 PKASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFVGATAASLLGLG 1581
            PKASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFVGATAASLLGLG
Sbjct: 1501 PKASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFVGATAASLLGLG 1560

BLAST of Cp4.1LG02g08540 vs. NCBI nr
Match: XP_022940627.1 (uncharacterized protein LOC111446165 isoform X1 [Cucurbita moschata] >XP_022940628.1 uncharacterized protein LOC111446165 isoform X1 [Cucurbita moschata])

HSP 1 Score: 2832 bits (7342), Expect = 0.0
Identity = 1538/1583 (97.16%), Postives = 1548/1583 (97.79%), Query Frame = 0

Query: 1    MMADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60
            MMADMDVHADVSQV EKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA
Sbjct: 1    MMADMDVHADVSQVNEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60

Query: 61   ATSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120
            ATSDDHVVSDLNGQ E SHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL
Sbjct: 61   ATSDDHVVSDLNGQPERSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120

Query: 121  KVSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGV 180
            KVSDAIQDETRVVDVLQSSDAKVDE EPGLDSSQKVEETR LENQAVNESFDVEGEIHGV
Sbjct: 121  KVSDAIQDETRVVDVLQSSDAKVDEAEPGLDSSQKVEETRFLENQAVNESFDVEGEIHGV 180

Query: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240
            DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS
Sbjct: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240

Query: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300
            NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE
Sbjct: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300

Query: 301  ENGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360
            ENGRVK FNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE
Sbjct: 301  ENGRVKTFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360

Query: 361  SSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420
            SSQKGDDILNDE NGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG
Sbjct: 361  SSQKGDDILNDEVNGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420

Query: 421  EGEDIKLNGQNEIVDAVKSSATLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSF 480
            EGEDIKLNGQNEIV AVKSSAT+DKRGQGE VPEDNETVAINELSDTIENRSEETELDSF
Sbjct: 421  EGEDIKLNGQNEIVGAVKSSATVDKRGQGEVVPEDNETVAINELSDTIENRSEETELDSF 480

Query: 481  EREEGIRESQDANVEAADCHSGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHP 540
            EREEGIRESQDANVEAADCHSGK+KVDEMVNKAATSDPVGGLGE QIISMGAVKSELDH 
Sbjct: 481  EREEGIRESQDANVEAADCHSGKEKVDEMVNKAATSDPVGGLGEYQIISMGAVKSELDHS 540

Query: 541  DGSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENK 600
            DGSVEDVKGECKLGVALNEENSERTQVTIN DGEHCHVVGE        DSLLEPSEENK
Sbjct: 541  DGSVEDVKGECKLGVALNEENSERTQVTINQDGEHCHVVGE--------DSLLEPSEENK 600

Query: 601  VDMEQHLAAAPSPLVNSEDVNGSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETL 660
            VDMEQHLAAAPSPLVNSED+NGSISTSTATSMDQDDP KTIDDKDTVANTSSFHDHTETL
Sbjct: 601  VDMEQHLAAAPSPLVNSEDLNGSISTSTATSMDQDDPSKTIDDKDTVANTSSFHDHTETL 660

Query: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKVDEC 720
            SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETAS+PKVDEC
Sbjct: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASNPKVDEC 720

Query: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780
            DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS
Sbjct: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780

Query: 781  VEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFE 840
            VEIREMSCTH LDDPVLRPDLEAEDCTMSENVASAGDYV LDNEVRENHEISLLGDNNFE
Sbjct: 781  VEIREMSCTHSLDDPVLRPDLEAEDCTMSENVASAGDYVHLDNEVRENHEISLLGDNNFE 840

Query: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900
            TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE
Sbjct: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900

Query: 901  TDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960
            TDSEFRDNERSSSPTA EKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN
Sbjct: 901  TDSEFRDNERSSSPTAIEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960

Query: 961  PMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020
            PMSDIASQTDSKPTKEET VVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR
Sbjct: 961  PMSDIASQTDSKPTKEETVVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020

Query: 1021 VPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARD 1080
            VPKFGDVNIREQIKCAQTEVD+TTK RDAIRVQIQIMRAAWKVLSD+LEAAVSEGRAARD
Sbjct: 1021 VPKFGDVNIREQIKCAQTEVDRTTKGRDAIRVQIQIMRAAWKVLSDNLEAAVSEGRAARD 1080

Query: 1081 LLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140
            LLR KRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK
Sbjct: 1081 LLRSKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140

Query: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICND 1200
            QLREQLSSTTGKQDELQQALDQKDQFEERLKLLR EMDLLRGNVLKAESVIKAAKKICND
Sbjct: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRMEMDLLRGNVLKAESVIKAAKKICND 1200

Query: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVE 1260
            ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIA+SGDVE
Sbjct: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIASSGDVE 1260

Query: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIV 1320
            RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALN IV
Sbjct: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNHIV 1320

Query: 1321 KERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNG 1380
            KER ARD+SLSTVSKTLEPE LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVAL+NG
Sbjct: 1321 KERQARDNSLSTVSKTLEPEKLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALVNG 1380

Query: 1381 LRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440
            LRNISCENDVEEPP+PVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL
Sbjct: 1381 LRNISCENDVEEPPRPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440

Query: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIIT 1500
            ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAE EAGNGLEEKESGIIT
Sbjct: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAEAEAGNGLEEKESGIIT 1500

Query: 1501 ESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIPPPLRNRSKRG-MQPWMWVLL 1560
            ESTPKEVSENTGKQGTAAKRP KASQSQYTKHSKTKSTIPPPLRNRSKRG MQPWMWVLL
Sbjct: 1501 ESTPKEVSENTGKQGTAAKRPQKASQSQYTKHSKTKSTIPPPLRNRSKRGGMQPWMWVLL 1560

Query: 1561 TTLIVFVVFFVGATAASLLGLGF 1582
            TTLIV VVFFVGATAASLLGLGF
Sbjct: 1561 TTLIVSVVFFVGATAASLLGLGF 1575

BLAST of Cp4.1LG02g08540 vs. NCBI nr
Match: KAG7037983.1 (Squamosa promoter-binding-like protein 14, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2807 bits (7277), Expect = 0.0
Identity = 1514/1555 (97.36%), Postives = 1526/1555 (98.14%), Query Frame = 0

Query: 2    MADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDAA 61
            MADMDVHADVSQV EKCALDMSTYENLPNACNAASLDSAAS+PKDDTDASYVFVNSTDAA
Sbjct: 1    MADMDVHADVSQVNEKCALDMSTYENLPNACNAASLDSAASVPKDDTDASYVFVNSTDAA 60

Query: 62   TSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLK 121
            TSDDHVVSDLNGQ ECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLK
Sbjct: 61   TSDDHVVSDLNGQPECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLK 120

Query: 122  VSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGVD 181
            VSDAIQDETRVVDVLQSSDAKVDE EPGLDSSQKVEETRILENQAVNESFDVEGEIHGVD
Sbjct: 121  VSDAIQDETRVVDVLQSSDAKVDEAEPGLDSSQKVEETRILENQAVNESFDVEGEIHGVD 180

Query: 182  APQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSN 241
            APQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSN
Sbjct: 181  APQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSN 240

Query: 242  TKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEE 301
            TKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEE
Sbjct: 241  TKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEE 300

Query: 302  NGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVES 361
            NGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVES
Sbjct: 301  NGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVES 360

Query: 362  SQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGE 421
            SQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGE
Sbjct: 361  SQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGE 420

Query: 422  GEDIKLNGQNEIVDAVKSSATLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSFE 481
            GEDIKLNGQNEIVDAVKSSAT+DKRGQGE VPEDNETVAINELSDTIENRSEETELDSFE
Sbjct: 421  GEDIKLNGQNEIVDAVKSSATMDKRGQGEVVPEDNETVAINELSDTIENRSEETELDSFE 480

Query: 482  REEGIRESQDANVEAADCHSGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHPD 541
            REEGIRESQDANVEAADCHSGK+K DEMVNKA TSDPVGGLGE QIISMGAVKSELDH D
Sbjct: 481  REEGIRESQDANVEAADCHSGKEKFDEMVNKAETSDPVGGLGEYQIISMGAVKSELDHSD 540

Query: 542  GSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENKV 601
            GSVEDVKG+CKLGVALNEENSERTQVTIN DGEHCHVVGE        DSLLEPSEENKV
Sbjct: 541  GSVEDVKGKCKLGVALNEENSERTQVTINQDGEHCHVVGE--------DSLLEPSEENKV 600

Query: 602  DMEQHLAAAPSPLVNSEDVNGSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETLS 661
            DMEQHLAAAPSPLVNSED+NGSISTSTATSMDQDDP KTIDDKDTVANTSSFHDHTETLS
Sbjct: 601  DMEQHLAAAPSPLVNSEDLNGSISTSTATSMDQDDPSKTIDDKDTVANTSSFHDHTETLS 660

Query: 662  SSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKVDECD 721
            SSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASH KVDECD
Sbjct: 661  SSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHLKVDECD 720

Query: 722  RVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSV 781
            RVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSV
Sbjct: 721  RVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSV 780

Query: 782  EIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFET 841
            EIREMSCTH LDDPVLRPDLEAEDCT+SENVASAGDYVQLDNEVRENHEISLLGDNNFET
Sbjct: 781  EIREMSCTHSLDDPVLRPDLEAEDCTISENVASAGDYVQLDNEVRENHEISLLGDNNFET 840

Query: 842  KSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVET 901
            KSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVET
Sbjct: 841  KSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVET 900

Query: 902  DSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLNP 961
            DSEFRDNERSSSPTANEKSGENIEIASSVGGGGRD TSDDCTSETEVKGSAV DEVDLNP
Sbjct: 901  DSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDKTSDDCTSETEVKGSAVKDEVDLNP 960

Query: 962  MSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRV 1021
            MSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRV
Sbjct: 961  MSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRV 1020

Query: 1022 PKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARDL 1081
            PKFGDVNIREQIKCAQTEVD+TTKDRDAIRVQIQIMRAAWKVLSD+LEAAVSEGRAARDL
Sbjct: 1021 PKFGDVNIREQIKCAQTEVDRTTKDRDAIRVQIQIMRAAWKVLSDNLEAAVSEGRAARDL 1080

Query: 1082 LRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQ 1141
            LR KRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQ
Sbjct: 1081 LRSKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQ 1140

Query: 1142 LREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICNDE 1201
            LREQLSSTTGKQDELQQALDQKDQFEERLKLLR EMDLLRGNVLKAESVIKAAKKICNDE
Sbjct: 1141 LREQLSSTTGKQDELQQALDQKDQFEERLKLLRMEMDLLRGNVLKAESVIKAAKKICNDE 1200

Query: 1202 SLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVER 1261
            SLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIA+SGDVER
Sbjct: 1201 SLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIASSGDVER 1260

Query: 1262 LQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIVK 1321
            LQRFSVNQVECMMELWN NAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALN IVK
Sbjct: 1261 LQRFSVNQVECMMELWNINAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNHIVK 1320

Query: 1322 ERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNGL 1381
            ERPARD+SLSTVSKTLEPE LIPAENMRDNDKPVIEVVKTKNQPTKNKKP TVVAL+NGL
Sbjct: 1321 ERPARDNSLSTVSKTLEPEKLIPAENMRDNDKPVIEVVKTKNQPTKNKKPMTVVALVNGL 1380

Query: 1382 RNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALE 1441
            RNISCENDVEEPP+PVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALE
Sbjct: 1381 RNISCENDVEEPPRPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALE 1440

Query: 1442 RKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITE 1501
            RKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITE
Sbjct: 1441 RKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITE 1500

Query: 1502 STPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMW 1556
            STPKEVSENTGKQGTAAKRP KASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMW
Sbjct: 1501 STPKEVSENTGKQGTAAKRPQKASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMW 1547

BLAST of Cp4.1LG02g08540 vs. NCBI nr
Match: KAG6608667.1 (Proton pump-interactor 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2733 bits (7084), Expect = 0.0
Identity = 1488/1561 (95.32%), Postives = 1500/1561 (96.09%), Query Frame = 0

Query: 22   MSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDAATSDDHVVSDLNGQLECSHNV 81
            MSTYENLPNACNAASLDSAAS+PKDDTDASYVFVNSTDAATSDDHVVSDLNGQ ECSHNV
Sbjct: 1    MSTYENLPNACNAASLDSAASVPKDDTDASYVFVNSTDAATSDDHVVSDLNGQPECSHNV 60

Query: 82   DTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLKVSDAIQDETRVVDVLQSSDA 141
            DTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLKVSDAIQDETRVVDVLQSSDA
Sbjct: 61   DTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLKVSDAIQDETRVVDVLQSSDA 120

Query: 142  KVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGVDAPQEDENVQIQEENQIISTS 201
            KVDE EPGLDSSQKVEETRILENQAVNESFDVEGEIHGVD PQEDENVQIQEENQIISTS
Sbjct: 121  KVDEAEPGLDSSQKVEETRILENQAVNESFDVEGEIHGVDTPQEDENVQIQEENQIISTS 180

Query: 202  ESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSNTKANRGIDIEAESSQNAEGI 261
            ESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSNTKANRGIDIEAESSQNAEGI
Sbjct: 181  ESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSSNTKANRGIDIEAESSQNAEGI 240

Query: 262  QFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEENGRVKAFNLTDTAANLRGKI 321
            QFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEENGRVKAFNLTD AANLRGKI
Sbjct: 241  QFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHEENGRVKAFNLTDIAANLRGKI 300

Query: 322  KLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVESSQKGDDILNDEENGRVKAFN 381
            KLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVESSQKGDDILNDEENGRVKAFN
Sbjct: 301  KLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVESSQKGDDILNDEENGRVKAFN 360

Query: 382  LSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGEGEDIKLNGQNEIVDAVKSSA 441
            LSDTEVDIQNHKENGIMKAPKLSNTE NPRSEIEVESPGEGEDIKLNGQNEIVDAVKSSA
Sbjct: 361  LSDTEVDIQNHKENGIMKAPKLSNTEVNPRSEIEVESPGEGEDIKLNGQNEIVDAVKSSA 420

Query: 442  TLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSFEREEGIRESQDANVEAADCHS 501
            T+DKRGQGE VPEDNETVAINELSDTIENRSEETELDSFEREEGIRESQDANVEAADCHS
Sbjct: 421  TMDKRGQGEVVPEDNETVAINELSDTIENRSEETELDSFEREEGIRESQDANVEAADCHS 480

Query: 502  GKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHPDGSVEDVKGECKLGVALNEEN 561
            GK+K DEMVNKAATSDPVGGLGE QIISMGAVKSELDH DG                   
Sbjct: 481  GKEKFDEMVNKAATSDPVGGLGEYQIISMGAVKSELDHSDG------------------- 540

Query: 562  SERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENKVDMEQHLAAAPSPLVNSEDVN 621
                        EHCHVVGE        DSLLEPSEENKVDMEQHLAAAPSPLVNSED+N
Sbjct: 541  ------------EHCHVVGE--------DSLLEPSEENKVDMEQHLAAAPSPLVNSEDLN 600

Query: 622  GSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETLSSSVDPDIDSVETHKLTHTML 681
            GSISTSTATSMDQDDP KTIDDKDTVANTSSFHDHTETLSSSVDPDIDSVETHKLTHTML
Sbjct: 601  GSISTSTATSMDQDDPSKTIDDKDTVANTSSFHDHTETLSSSVDPDIDSVETHKLTHTML 660

Query: 682  INDPKVELNEITVNEQEVNHVLELEETSETASHPKVDECDRVEVLEGTVSGDEMSIALDE 741
            INDPKVELNEITVN+QEVNHVLELEETSETASH KVDECDRVEVLEGTVSGDEMSIALDE
Sbjct: 661  INDPKVELNEITVNDQEVNHVLELEETSETASHLKVDECDRVEVLEGTVSGDEMSIALDE 720

Query: 742  SRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSVEIREMSCTHGLDDPVLRPDL 801
            SRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSVEIREMSCTH LDDPVLRPDL
Sbjct: 721  SRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTSVEIREMSCTHSLDDPVLRPDL 780

Query: 802  EAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFETKSESGDIEEENQSTFPCNDM 861
            EAEDCT+SENVASAGDYVQLDNEVRENHEISLLGDNNFETKSESGDIEEENQSTFPCNDM
Sbjct: 781  EAEDCTISENVASAGDYVQLDNEVRENHEISLLGDNNFETKSESGDIEEENQSTFPCNDM 840

Query: 862  RSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVETDSEFRDNERSSSPTANEKSG 921
            RSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVETDSEFRDNERSSSPTANEKSG
Sbjct: 841  RSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVETDSEFRDNERSSSPTANEKSG 900

Query: 922  ENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLNPMSDIASQTDSKPTKEETEVV 981
            ENIEIASSVGGGGRD TSDDCTSETEVKGSAV DEVDLNPMSDIASQTDSKPTKEETEVV
Sbjct: 901  ENIEIASSVGGGGRDKTSDDCTSETEVKGSAVKDEVDLNPMSDIASQTDSKPTKEETEVV 960

Query: 982  HESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVD 1041
            HESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVD
Sbjct: 961  HESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVD 1020

Query: 1042 QTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARDLLRFKRQEIDSVQSVITKVKN 1101
            +TTKDRDAIRVQIQIMRAAWKVLSD+LEAAVSEGRAARDLLR KRQEIDSVQSVITKVKN
Sbjct: 1021 RTTKDRDAIRVQIQIMRAAWKVLSDNLEAAVSEGRAARDLLRSKRQEIDSVQSVITKVKN 1080

Query: 1102 AMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALD 1161
            AMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALD
Sbjct: 1081 AMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALD 1140

Query: 1162 QKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQE 1221
            QKDQFEERLKLLR EMDLLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQE
Sbjct: 1141 QKDQFEERLKLLRMEMDLLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQE 1200

Query: 1222 AYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNA 1281
            AYANLQSTRKQLYDKNKYYWKYRDDVKEANEIA+SGDVERLQRFSVNQVECMMELWN NA
Sbjct: 1201 AYANLQSTRKQLYDKNKYYWKYRDDVKEANEIASSGDVERLQRFSVNQVECMMELWNINA 1260

Query: 1282 EFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPEN 1341
            EFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALN IVKERPARD+SLSTVSKTLEPE 
Sbjct: 1261 EFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNHIVKERPARDNSLSTVSKTLEPEK 1320

Query: 1342 LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKR 1401
            LIPAENMRDNDKPVIEVVKTKNQPTKNKKP TVVAL+NGLRNISCENDVEEPP+PVEIKR
Sbjct: 1321 LIPAENMRDNDKPVIEVVKTKNQPTKNKKPMTVVALVNGLRNISCENDVEEPPRPVEIKR 1380

Query: 1402 TREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKE 1461
            TREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKE
Sbjct: 1381 TREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKE 1440

Query: 1462 AEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP 1521
            AEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP
Sbjct: 1441 AEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP 1500

Query: 1522 PKASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFVGATAASLLGLG 1581
             KASQSQYTKHSKTKSTIPPPLRNRSKRGMQ WMWVLLTTLIVFVVFFVGATAASLLGLG
Sbjct: 1501 QKASQSQYTKHSKTKSTIPPPLRNRSKRGMQQWMWVLLTTLIVFVVFFVGATAASLLGLG 1522

BLAST of Cp4.1LG02g08540 vs. NCBI nr
Match: XP_022940629.1 (uncharacterized protein LOC111446165 isoform X2 [Cucurbita moschata])

HSP 1 Score: 2712 bits (7031), Expect = 0.0
Identity = 1489/1583 (94.06%), Postives = 1500/1583 (94.76%), Query Frame = 0

Query: 1    MMADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60
            MMADMDVHADVSQV EKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA
Sbjct: 1    MMADMDVHADVSQVNEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60

Query: 61   ATSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120
            ATSDDHVVSDLNGQ E SHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL
Sbjct: 61   ATSDDHVVSDLNGQPERSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120

Query: 121  KVSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGV 180
            KVSDAIQDETRVVDVLQSSDAKVDE EPGLDSSQKVEETR LENQAVNESFDVEGEIHGV
Sbjct: 121  KVSDAIQDETRVVDVLQSSDAKVDEAEPGLDSSQKVEETRFLENQAVNESFDVEGEIHGV 180

Query: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240
            DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS
Sbjct: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240

Query: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300
            NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE
Sbjct: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300

Query: 301  ENGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360
            ENGRVK FNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE
Sbjct: 301  ENGRVKTFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360

Query: 361  SSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420
            SSQKGDDILNDE NGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG
Sbjct: 361  SSQKGDDILNDEVNGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420

Query: 421  EGEDIKLNGQNEIVDAVKSSATLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSF 480
            EGEDIKLNGQNEIV AVKSSAT+DKRGQGE VPEDNETVAINELSDTIENRSEETELDSF
Sbjct: 421  EGEDIKLNGQNEIVGAVKSSATVDKRGQGEVVPEDNETVAINELSDTIENRSEETELDSF 480

Query: 481  EREEGIRESQDANVEAADCHSGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHP 540
            EREEGIRESQDANVEAADCHSGK+KVDEMVNKAATSDPVGGLGE QII            
Sbjct: 481  EREEGIRESQDANVEAADCHSGKEKVDEMVNKAATSDPVGGLGEYQII------------ 540

Query: 541  DGSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENK 600
                                                  +GE        DSLLEPSEENK
Sbjct: 541  --------------------------------------IGE--------DSLLEPSEENK 600

Query: 601  VDMEQHLAAAPSPLVNSEDVNGSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETL 660
            VDMEQHLAAAPSPLVNSED+NGSISTSTATSMDQDDP KTIDDKDTVANTSSFHDHTETL
Sbjct: 601  VDMEQHLAAAPSPLVNSEDLNGSISTSTATSMDQDDPSKTIDDKDTVANTSSFHDHTETL 660

Query: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKVDEC 720
            SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETAS+PKVDEC
Sbjct: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASNPKVDEC 720

Query: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780
            DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS
Sbjct: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780

Query: 781  VEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFE 840
            VEIREMSCTH LDDPVLRPDLEAEDCTMSENVASAGDYV LDNEVRENHEISLLGDNNFE
Sbjct: 781  VEIREMSCTHSLDDPVLRPDLEAEDCTMSENVASAGDYVHLDNEVRENHEISLLGDNNFE 840

Query: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900
            TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE
Sbjct: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900

Query: 901  TDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960
            TDSEFRDNERSSSPTA EKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN
Sbjct: 901  TDSEFRDNERSSSPTAIEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960

Query: 961  PMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020
            PMSDIASQTDSKPTKEET VVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR
Sbjct: 961  PMSDIASQTDSKPTKEETVVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020

Query: 1021 VPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARD 1080
            VPKFGDVNIREQIKCAQTEVD+TTK RDAIRVQIQIMRAAWKVLSD+LEAAVSEGRAARD
Sbjct: 1021 VPKFGDVNIREQIKCAQTEVDRTTKGRDAIRVQIQIMRAAWKVLSDNLEAAVSEGRAARD 1080

Query: 1081 LLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140
            LLR KRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK
Sbjct: 1081 LLRSKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140

Query: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICND 1200
            QLREQLSSTTGKQDELQQALDQKDQFEERLKLLR EMDLLRGNVLKAESVIKAAKKICND
Sbjct: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRMEMDLLRGNVLKAESVIKAAKKICND 1200

Query: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVE 1260
            ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIA+SGDVE
Sbjct: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIASSGDVE 1260

Query: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIV 1320
            RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALN IV
Sbjct: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNHIV 1320

Query: 1321 KERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNG 1380
            KER ARD+SLSTVSKTLEPE LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVAL+NG
Sbjct: 1321 KERQARDNSLSTVSKTLEPEKLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALVNG 1380

Query: 1381 LRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440
            LRNISCENDVEEPP+PVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL
Sbjct: 1381 LRNISCENDVEEPPRPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440

Query: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIIT 1500
            ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAE EAGNGLEEKESGIIT
Sbjct: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAEAEAGNGLEEKESGIIT 1500

Query: 1501 ESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIPPPLRNRSKRG-MQPWMWVLL 1560
            ESTPKEVSENTGKQGTAAKRP KASQSQYTKHSKTKSTIPPPLRNRSKRG MQPWMWVLL
Sbjct: 1501 ESTPKEVSENTGKQGTAAKRPQKASQSQYTKHSKTKSTIPPPLRNRSKRGGMQPWMWVLL 1525

Query: 1561 TTLIVFVVFFVGATAASLLGLGF 1582
            TTLIV VVFFVGATAASLLGLGF
Sbjct: 1561 TTLIVSVVFFVGATAASLLGLGF 1525

BLAST of Cp4.1LG02g08540 vs. ExPASy TrEMBL
Match: A0A6J1FPU2 (uncharacterized protein LOC111446165 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446165 PE=4 SV=1)

HSP 1 Score: 2832 bits (7342), Expect = 0.0
Identity = 1538/1583 (97.16%), Postives = 1548/1583 (97.79%), Query Frame = 0

Query: 1    MMADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60
            MMADMDVHADVSQV EKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA
Sbjct: 1    MMADMDVHADVSQVNEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60

Query: 61   ATSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120
            ATSDDHVVSDLNGQ E SHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL
Sbjct: 61   ATSDDHVVSDLNGQPERSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120

Query: 121  KVSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGV 180
            KVSDAIQDETRVVDVLQSSDAKVDE EPGLDSSQKVEETR LENQAVNESFDVEGEIHGV
Sbjct: 121  KVSDAIQDETRVVDVLQSSDAKVDEAEPGLDSSQKVEETRFLENQAVNESFDVEGEIHGV 180

Query: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240
            DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS
Sbjct: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240

Query: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300
            NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE
Sbjct: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300

Query: 301  ENGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360
            ENGRVK FNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE
Sbjct: 301  ENGRVKTFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360

Query: 361  SSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420
            SSQKGDDILNDE NGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG
Sbjct: 361  SSQKGDDILNDEVNGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420

Query: 421  EGEDIKLNGQNEIVDAVKSSATLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSF 480
            EGEDIKLNGQNEIV AVKSSAT+DKRGQGE VPEDNETVAINELSDTIENRSEETELDSF
Sbjct: 421  EGEDIKLNGQNEIVGAVKSSATVDKRGQGEVVPEDNETVAINELSDTIENRSEETELDSF 480

Query: 481  EREEGIRESQDANVEAADCHSGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHP 540
            EREEGIRESQDANVEAADCHSGK+KVDEMVNKAATSDPVGGLGE QIISMGAVKSELDH 
Sbjct: 481  EREEGIRESQDANVEAADCHSGKEKVDEMVNKAATSDPVGGLGEYQIISMGAVKSELDHS 540

Query: 541  DGSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENK 600
            DGSVEDVKGECKLGVALNEENSERTQVTIN DGEHCHVVGE        DSLLEPSEENK
Sbjct: 541  DGSVEDVKGECKLGVALNEENSERTQVTINQDGEHCHVVGE--------DSLLEPSEENK 600

Query: 601  VDMEQHLAAAPSPLVNSEDVNGSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETL 660
            VDMEQHLAAAPSPLVNSED+NGSISTSTATSMDQDDP KTIDDKDTVANTSSFHDHTETL
Sbjct: 601  VDMEQHLAAAPSPLVNSEDLNGSISTSTATSMDQDDPSKTIDDKDTVANTSSFHDHTETL 660

Query: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKVDEC 720
            SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETAS+PKVDEC
Sbjct: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASNPKVDEC 720

Query: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780
            DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS
Sbjct: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780

Query: 781  VEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFE 840
            VEIREMSCTH LDDPVLRPDLEAEDCTMSENVASAGDYV LDNEVRENHEISLLGDNNFE
Sbjct: 781  VEIREMSCTHSLDDPVLRPDLEAEDCTMSENVASAGDYVHLDNEVRENHEISLLGDNNFE 840

Query: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900
            TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE
Sbjct: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900

Query: 901  TDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960
            TDSEFRDNERSSSPTA EKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN
Sbjct: 901  TDSEFRDNERSSSPTAIEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960

Query: 961  PMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020
            PMSDIASQTDSKPTKEET VVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR
Sbjct: 961  PMSDIASQTDSKPTKEETVVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020

Query: 1021 VPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARD 1080
            VPKFGDVNIREQIKCAQTEVD+TTK RDAIRVQIQIMRAAWKVLSD+LEAAVSEGRAARD
Sbjct: 1021 VPKFGDVNIREQIKCAQTEVDRTTKGRDAIRVQIQIMRAAWKVLSDNLEAAVSEGRAARD 1080

Query: 1081 LLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140
            LLR KRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK
Sbjct: 1081 LLRSKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140

Query: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICND 1200
            QLREQLSSTTGKQDELQQALDQKDQFEERLKLLR EMDLLRGNVLKAESVIKAAKKICND
Sbjct: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRMEMDLLRGNVLKAESVIKAAKKICND 1200

Query: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVE 1260
            ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIA+SGDVE
Sbjct: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIASSGDVE 1260

Query: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIV 1320
            RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALN IV
Sbjct: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNHIV 1320

Query: 1321 KERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNG 1380
            KER ARD+SLSTVSKTLEPE LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVAL+NG
Sbjct: 1321 KERQARDNSLSTVSKTLEPEKLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALVNG 1380

Query: 1381 LRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440
            LRNISCENDVEEPP+PVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL
Sbjct: 1381 LRNISCENDVEEPPRPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440

Query: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIIT 1500
            ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAE EAGNGLEEKESGIIT
Sbjct: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAEAEAGNGLEEKESGIIT 1500

Query: 1501 ESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIPPPLRNRSKRG-MQPWMWVLL 1560
            ESTPKEVSENTGKQGTAAKRP KASQSQYTKHSKTKSTIPPPLRNRSKRG MQPWMWVLL
Sbjct: 1501 ESTPKEVSENTGKQGTAAKRPQKASQSQYTKHSKTKSTIPPPLRNRSKRGGMQPWMWVLL 1560

Query: 1561 TTLIVFVVFFVGATAASLLGLGF 1582
            TTLIV VVFFVGATAASLLGLGF
Sbjct: 1561 TTLIVSVVFFVGATAASLLGLGF 1575

BLAST of Cp4.1LG02g08540 vs. ExPASy TrEMBL
Match: A0A6J1FKT5 (uncharacterized protein LOC111446165 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446165 PE=4 SV=1)

HSP 1 Score: 2712 bits (7031), Expect = 0.0
Identity = 1489/1583 (94.06%), Postives = 1500/1583 (94.76%), Query Frame = 0

Query: 1    MMADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60
            MMADMDVHADVSQV EKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA
Sbjct: 1    MMADMDVHADVSQVNEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60

Query: 61   ATSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120
            ATSDDHVVSDLNGQ E SHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL
Sbjct: 61   ATSDDHVVSDLNGQPERSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120

Query: 121  KVSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDVEGEIHGV 180
            KVSDAIQDETRVVDVLQSSDAKVDE EPGLDSSQKVEETR LENQAVNESFDVEGEIHGV
Sbjct: 121  KVSDAIQDETRVVDVLQSSDAKVDEAEPGLDSSQKVEETRFLENQAVNESFDVEGEIHGV 180

Query: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240
            DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS
Sbjct: 181  DAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMKSS 240

Query: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300
            NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE
Sbjct: 241  NTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQNHE 300

Query: 301  ENGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360
            ENGRVK FNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE
Sbjct: 301  ENGRVKTFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIEVE 360

Query: 361  SSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420
            SSQKGDDILNDE NGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG
Sbjct: 361  SSQKGDDILNDEVNGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPG 420

Query: 421  EGEDIKLNGQNEIVDAVKSSATLDKRGQGEAVPEDNETVAINELSDTIENRSEETELDSF 480
            EGEDIKLNGQNEIV AVKSSAT+DKRGQGE VPEDNETVAINELSDTIENRSEETELDSF
Sbjct: 421  EGEDIKLNGQNEIVGAVKSSATVDKRGQGEVVPEDNETVAINELSDTIENRSEETELDSF 480

Query: 481  EREEGIRESQDANVEAADCHSGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSELDHP 540
            EREEGIRESQDANVEAADCHSGK+KVDEMVNKAATSDPVGGLGE QII            
Sbjct: 481  EREEGIRESQDANVEAADCHSGKEKVDEMVNKAATSDPVGGLGEYQII------------ 540

Query: 541  DGSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENK 600
                                                  +GE        DSLLEPSEENK
Sbjct: 541  --------------------------------------IGE--------DSLLEPSEENK 600

Query: 601  VDMEQHLAAAPSPLVNSEDVNGSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHTETL 660
            VDMEQHLAAAPSPLVNSED+NGSISTSTATSMDQDDP KTIDDKDTVANTSSFHDHTETL
Sbjct: 601  VDMEQHLAAAPSPLVNSEDLNGSISTSTATSMDQDDPSKTIDDKDTVANTSSFHDHTETL 660

Query: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKVDEC 720
            SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETAS+PKVDEC
Sbjct: 661  SSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASNPKVDEC 720

Query: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780
            DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS
Sbjct: 721  DRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIGNTS 780

Query: 781  VEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENHEISLLGDNNFE 840
            VEIREMSCTH LDDPVLRPDLEAEDCTMSENVASAGDYV LDNEVRENHEISLLGDNNFE
Sbjct: 781  VEIREMSCTHSLDDPVLRPDLEAEDCTMSENVASAGDYVHLDNEVRENHEISLLGDNNFE 840

Query: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900
            TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE
Sbjct: 841  TKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQSSAVE 900

Query: 901  TDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960
            TDSEFRDNERSSSPTA EKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN
Sbjct: 901  TDSEFRDNERSSSPTAIEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDEVDLN 960

Query: 961  PMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020
            PMSDIASQTDSKPTKEET VVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR
Sbjct: 961  PMSDIASQTDSKPTKEETVVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNFLVR 1020

Query: 1021 VPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARD 1080
            VPKFGDVNIREQIKCAQTEVD+TTK RDAIRVQIQIMRAAWKVLSD+LEAAVSEGRAARD
Sbjct: 1021 VPKFGDVNIREQIKCAQTEVDRTTKGRDAIRVQIQIMRAAWKVLSDNLEAAVSEGRAARD 1080

Query: 1081 LLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140
            LLR KRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK
Sbjct: 1081 LLRSKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQLK 1140

Query: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICND 1200
            QLREQLSSTTGKQDELQQALDQKDQFEERLKLLR EMDLLRGNVLKAESVIKAAKKICND
Sbjct: 1141 QLREQLSSTTGKQDELQQALDQKDQFEERLKLLRMEMDLLRGNVLKAESVIKAAKKICND 1200

Query: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATSGDVE 1260
            ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIA+SGDVE
Sbjct: 1201 ESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIASSGDVE 1260

Query: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNQIV 1320
            RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALN IV
Sbjct: 1261 RLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHALNHIV 1320

Query: 1321 KERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALMNG 1380
            KER ARD+SLSTVSKTLEPE LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVAL+NG
Sbjct: 1321 KERQARDNSLSTVSKTLEPEKLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVALVNG 1380

Query: 1381 LRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440
            LRNISCENDVEEPP+PVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL
Sbjct: 1381 LRNISCENDVEEPPRPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKAKEAL 1440

Query: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKESGIIT 1500
            ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAE EAGNGLEEKESGIIT
Sbjct: 1441 ERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAEAEAGNGLEEKESGIIT 1500

Query: 1501 ESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIPPPLRNRSKRG-MQPWMWVLL 1560
            ESTPKEVSENTGKQGTAAKRP KASQSQYTKHSKTKSTIPPPLRNRSKRG MQPWMWVLL
Sbjct: 1501 ESTPKEVSENTGKQGTAAKRPQKASQSQYTKHSKTKSTIPPPLRNRSKRGGMQPWMWVLL 1525

Query: 1561 TTLIVFVVFFVGATAASLLGLGF 1582
            TTLIV VVFFVGATAASLLGLGF
Sbjct: 1561 TTLIVSVVFFVGATAASLLGLGF 1525

BLAST of Cp4.1LG02g08540 vs. ExPASy TrEMBL
Match: A0A6J1J1D0 (uncharacterized protein LOC111480984 OS=Cucurbita maxima OX=3661 GN=LOC111480984 PE=4 SV=1)

HSP 1 Score: 2660 bits (6895), Expect = 0.0
Identity = 1464/1586 (92.31%), Postives = 1485/1586 (93.63%), Query Frame = 0

Query: 1    MMADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDA 60
            MMADMDVHADVS+V EKCALDMSTYENLPNACNA+ LDSAASIPKD+TDASYVFVNSTDA
Sbjct: 1    MMADMDVHADVSEVNEKCALDMSTYENLPNACNASPLDSAASIPKDNTDASYVFVNSTDA 60

Query: 61   ATSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHL 120
            ATSDDHVVSDLNGQ ECSHNVDTEIKVHNGEF TDDGRKPDSFFVSNASEDSPFKFSEHL
Sbjct: 61   ATSDDHVVSDLNGQPECSHNVDTEIKVHNGEFCTDDGRKPDSFFVSNASEDSPFKFSEHL 120

Query: 121  KVSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDVE---GEI 180
            KVSDAIQDETRVVDVLQSSDAKVDE EPGLDSSQKVEETRILENQAVNE FD E   GEI
Sbjct: 121  KVSDAIQDETRVVDVLQSSDAKVDEAEPGLDSSQKVEETRILENQAVNELFDAEKNSGEI 180

Query: 181  HGVDAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIM 240
            +GVDAPQEDENVQIQEENQIIST+ESSNTDANLGGQILVVSSQM EDIQIHEDNGVMGIM
Sbjct: 181  YGVDAPQEDENVQIQEENQIISTTESSNTDANLGGQILVVSSQMVEDIQIHEDNGVMGIM 240

Query: 241  KSSNTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQ 300
            KSSNTKAN GIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESS KADEVQ
Sbjct: 241  KSSNTKANHGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSRKADEVQ 300

Query: 301  NHEENGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKI 360
            NHEENGRVKAFNLTDTAA LRGKIKLESSKKADDIQN EEN RMKAFDLS TVANPRGKI
Sbjct: 301  NHEENGRVKAFNLTDTAATLRGKIKLESSKKADDIQNGEENVRMKAFDLSCTVANPRGKI 360

Query: 361  EVESSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVE 420
            EVESSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVE
Sbjct: 361  EVESSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVE 420

Query: 421  SPGEGEDIKLNGQNEIVDAVKSSATLDKRGQGEAVPEDNETVAINELSDTIENRSEETEL 480
            SPGEGEDIKLNGQNEIVDAVKSSAT+DKRGQGE VPEDNETVAINELSDTIENRSEETEL
Sbjct: 421  SPGEGEDIKLNGQNEIVDAVKSSATVDKRGQGEVVPEDNETVAINELSDTIENRSEETEL 480

Query: 481  DSFEREEGIRESQDANVEAADCHSGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKSEL 540
            DSFEREEGIRESQDANVEAADCHSGK+KVDEMVNKAA SDPVGGLGESQII         
Sbjct: 481  DSFEREEGIRESQDANVEAADCHSGKEKVDEMVNKAAISDPVGGLGESQII--------- 540

Query: 541  DHPDGSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSE 600
                                                             N EDSLLEPSE
Sbjct: 541  -------------------------------------------------NGEDSLLEPSE 600

Query: 601  ENKVDMEQHLAAAPSPLVNSEDVNGSISTSTATSMDQDDPLKTIDDKDTVANTSSFHDHT 660
            ENKVDMEQHLAAAPSPLVNSED+NGSISTSTATSMDQDDP KTIDDKDT ANTSSFHDHT
Sbjct: 601  ENKVDMEQHLAAAPSPLVNSEDLNGSISTSTATSMDQDDPSKTIDDKDTGANTSSFHDHT 660

Query: 661  ETLSSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKV 720
            ETLSSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKV
Sbjct: 661  ETLSSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSETASHPKV 720

Query: 721  DECDRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIG 780
            DECDRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIG
Sbjct: 721  DECDRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVIG 780

Query: 781  NTSVEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENHEISLL-GD 840
            NTSVEIR+MSCTH LDDPVLRPDLEAEDCTMSENVASA DYVQLDNEVRENHEISLL GD
Sbjct: 781  NTSVEIRKMSCTHSLDDPVLRPDLEAEDCTMSENVASADDYVQLDNEVRENHEISLLLGD 840

Query: 841  NNFETKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQS 900
            NNFETKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNG NKSTAIQQS
Sbjct: 841  NNFETKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGGNKSTAIQQS 900

Query: 901  SAVETDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVNDE 960
            SAVETDSEFRDNERS SPTANEKSGENIEIASSV GGGRDITSDDCTSETEVKGSAVNDE
Sbjct: 901  SAVETDSEFRDNERSLSPTANEKSGENIEIASSVVGGGRDITSDDCTSETEVKGSAVNDE 960

Query: 961  VDLNPMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLN 1020
            VDLNPMSDIASQTDSKPTKEETEVVH SCQNEPSPISPEGSIEALAGKNVGTEAGTKPLN
Sbjct: 961  VDLNPMSDIASQTDSKPTKEETEVVH-SCQNEPSPISPEGSIEALAGKNVGTEAGTKPLN 1020

Query: 1021 FLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGR 1080
            FLVRVPKFGDVNIREQIK AQ EVDQTTKDRDAIRVQIQI+RAAWKVLSD+LEAAVSEGR
Sbjct: 1021 FLVRVPKFGDVNIREQIKSAQMEVDQTTKDRDAIRVQIQIVRAAWKVLSDNLEAAVSEGR 1080

Query: 1081 AARDLLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIREI 1140
            AARDLL+ KRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEH IEHETLPLKEEKQLIREI
Sbjct: 1081 AARDLLKSKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHSIEHETLPLKEEKQLIREI 1140

Query: 1141 KQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKK 1200
            KQLKQLREQLSSTTGKQ+ELQQALDQKDQFEERL+LLRKEMDLLRGNVLKAESVIKAAKK
Sbjct: 1141 KQLKQLREQLSSTTGKQNELQQALDQKDQFEERLRLLRKEMDLLRGNVLKAESVIKAAKK 1200

Query: 1201 ICNDESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIATS 1260
            ICNDESLKLDELQSKFKAADKIRQEAYANLQS RKQLYDKNKYYWKYRDDVKEANEIA+S
Sbjct: 1201 ICNDESLKLDELQSKFKAADKIRQEAYANLQSLRKQLYDKNKYYWKYRDDVKEANEIASS 1260

Query: 1261 GDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHAL 1320
            GDVERLQRFSVNQVECMMELWNTNAEFREEYIK+NMRSTLRRLKTLDGRSLGPNEEPHAL
Sbjct: 1261 GDVERLQRFSVNQVECMMELWNTNAEFREEYIKTNMRSTLRRLKTLDGRSLGPNEEPHAL 1320

Query: 1321 NQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVA 1380
            N IVKERPARD+SLSTVSKTLEPE LIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVA
Sbjct: 1321 NHIVKERPARDNSLSTVSKTLEPEKLIPAENMRDNDKPVIEVVKTKNQPTKNKKPTTVVA 1380

Query: 1381 LMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKA 1440
            L+NGL NISCENDVEEPP+PVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKA
Sbjct: 1381 LVNGLGNISCENDVEEPPRPVEIKRTREEEELAAKAEELRKKEEAIKLKEQHKLEEKTKA 1440

Query: 1441 KEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEEKES 1500
            KEALERK RNAEKAHARA TKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEE+ES
Sbjct: 1441 KEALERKSRNAEKAHARAATKARKEAEEREKLREKRAKKKARKMAAAETEAGNGLEERES 1500

Query: 1501 GIITESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIPPPLRNRSKRGMQPWMW 1560
            GIITESTPKEVSENTGKQGTAAKRP KASQSQYTKHSKTKSTIPP LRNRSKRGMQPWMW
Sbjct: 1501 GIITESTPKEVSENTGKQGTAAKRPQKASQSQYTKHSKTKSTIPPHLRNRSKRGMQPWMW 1527

Query: 1561 VLLTTLIVFVVFFVGATAASLLGLGF 1582
            VLL TLIVFVVFFVGAT AS+LGLGF
Sbjct: 1561 VLLATLIVFVVFFVGATTASILGLGF 1527

BLAST of Cp4.1LG02g08540 vs. ExPASy TrEMBL
Match: A0A6J1F7N0 (uncharacterized protein LOC111442839 OS=Cucurbita moschata OX=3662 GN=LOC111442839 PE=4 SV=1)

HSP 1 Score: 1841 bits (4769), Expect = 0.0
Identity = 1084/1591 (68.13%), Postives = 1218/1591 (76.56%), Query Frame = 0

Query: 2    MADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDAA 61
            MADMDV AD S+V EKC LDMSTYENLP ACNA  LD A +IPKDDTD SYVFV+S DAA
Sbjct: 1    MADMDVLADFSEVNEKCTLDMSTYENLPKACNAEPLDCATAIPKDDTDGSYVFVSSNDAA 60

Query: 62   TSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLK 121
            TSDDHV SD+NGQ +CS NVDTEIKV +GE STDDGRKP+  FVS+A  DS F FSEH+ 
Sbjct: 61   TSDDHVASDVNGQAKCSQNVDTEIKVRDGELSTDDGRKPEPIFVSDALGDSQFNFSEHVN 120

Query: 122  VSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDV---EGEIH 181
             SDAI DE RV DVLQSSDAK DE EP L+ S  VEETRI E QA NESFD     GEIH
Sbjct: 121  ESDAILDEIRVADVLQSSDAKEDEAEPQLNFSPTVEETRIPEGQATNESFDAVTNSGEIH 180

Query: 182  GVDAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMK 241
            GV++P E E++ IQEENQIIST  SSN D+N  G++ V SSQMAEDIQIHEDNG++GIMK
Sbjct: 181  GVESPHETEDIHIQEENQIISTPGSSNPDSNRSGEVKVESSQMAEDIQIHEDNGIVGIMK 240

Query: 242  SSNTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQN 301
            SS+T+ N  I IEAESSQ A+ IQ  KE G VAI  SD ESN GEE EVESSLKAD+ QN
Sbjct: 241  SSDTEENHVIGIEAESSQKADSIQIHKENGTVAIMLSDTESNPGEETEVESSLKADDTQN 300

Query: 302  HEENGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIE 361
            HEENG VKAF+                                                 
Sbjct: 301  HEENGIVKAFD------------------------------------------------- 360

Query: 362  VESSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVES 421
                                                        LSNTEANPRSE+E ES
Sbjct: 361  --------------------------------------------LSNTEANPRSELEEES 420

Query: 422  PGEGEDIKLNGQNEIVDAVKSSATLDKRGQ-GEAVPEDNETVAINELSDTIENRSEETEL 481
              E EDI+L GQNEIV+  KSSA+++KRG+ GE +PEDNETV INELSDTI NRSEETE+
Sbjct: 421  SREVEDIELRGQNEIVETNKSSASMEKRGEEGEVIPEDNETVVINELSDTIPNRSEETEM 480

Query: 482  DSFEREEGIRESQDANVEAADCH--SGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKS 541
            +SFEREE IRESQDA +EAADC+  + ++KVDEMV+KA  SD VGG+GESQIIS+GA KS
Sbjct: 481  ESFEREESIRESQDATMEAADCNCVNVQEKVDEMVSKAVVSDSVGGIGESQIISLGAAKS 540

Query: 542  ELDHPDGSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEP 601
            E+DH D SVEDVKGECK GVALNEEN E TQ+TI+ DGE   V GEEQESLNNE SLLEP
Sbjct: 541  EVDHLDDSVEDVKGECKQGVALNEENPESTQITISQDGERFQVAGEEQESLNNELSLLEP 600

Query: 602  SEENKVDMEQHLAAAPSPLVNSEDVNGSISTST----ATSMDQDDPLKTIDDKDTVANTS 661
            SEENK DMEQ L A PSPLV  ED+NGS+   T     TSMDQDDPL+ IDDKDT+ N +
Sbjct: 601  SEENKADMEQDLEATPSPLVCPEDINGSMPIRTDDGLPTSMDQDDPLEAIDDKDTIVNRT 660

Query: 662  SFHDHTETLSSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSET 721
            SFHD TE+   SVD DI + ETH L+ TM I+DP+VELNEITVNEQ VNHV ELEE SET
Sbjct: 661  SFHDLTESSPGSVDYDIATDETHILSPTMFISDPRVELNEITVNEQVVNHVFELEENSET 720

Query: 722  ASHPKVDECDRVEVLEGTVSG--DEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVET 781
             S PKVDEC +V  LE TVSG  D+M  ALD+SR + GD+SVAGSQLIP ++E  +S+ET
Sbjct: 721  VSQPKVDECIKVGDLECTVSGNGDDMPTALDQSRIACGDNSVAGSQLIPEDIERVESIET 780

Query: 782  AVSSVVIGNTSVEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENH 841
            AVS VVIGNT +EIRE    + L+DP LR DL  E CTMSENVASAGD V  D EV ENH
Sbjct: 781  AVSIVVIGNTKIEIRETPSVNCLNDPFLRSDLRVEHCTMSENVASAGDDVLPDQEVSENH 840

Query: 842  EISLLGDNNFETKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNK 901
            E  LLG++NFE K E+G IE+++QSTF  NDMRS+S D  SIE EERGSTVPEVPNG  K
Sbjct: 841  EDDLLGNSNFEIKCENGHIEKDDQSTFHSNDMRSKSKDCTSIENEERGSTVPEVPNGVVK 900

Query: 902  STAIQQSSAVETDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCT-SETEV 961
            S  I QSSAVET SE  DN+ SSSPTANE S ++IEI SS+GGG R I  DDC+ S+TEV
Sbjct: 901  SPEIPQSSAVETGSELHDNKSSSSPTANENSVDDIEITSSIGGGSRTIPGDDCSVSKTEV 960

Query: 962  -KGSAVNDEVDLNPMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVG 1021
             K S + DE  LN +SD+  +TD K TK+ETEV+HE CQNEPSP+SPEGS +AL G+NVG
Sbjct: 961  LKRSMIKDEGSLNSISDVVFETDGKLTKDETEVIHEDCQNEPSPVSPEGSADALTGQNVG 1020

Query: 1022 TEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDD 1081
             EAGT+P NFLV+VP+F D NIREQIKCAQTEVD+ TKDRDAIRVQIQ MRAA KVLSD+
Sbjct: 1021 AEAGTRPFNFLVKVPRFDDQNIREQIKCAQTEVDRKTKDRDAIRVQIQTMRAACKVLSDN 1080

Query: 1082 LEAAVSEGRAARDLLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLK 1141
            LEAA+SEGRAARDLL+ KR EIDSVQSVITKVKNAMS+ DIDGR+R+ EH+IEHETLPLK
Sbjct: 1081 LEAAMSEGRAARDLLKSKRLEIDSVQSVITKVKNAMSVEDIDGRIRNTEHMIEHETLPLK 1140

Query: 1142 EEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKA 1201
            EEKQL+REIKQLKQ REQLSST GKQ+ELQQALDQ+DQ EERLKLLRKEMDLLRGNVLKA
Sbjct: 1141 EEKQLLREIKQLKQQREQLSSTMGKQEELQQALDQRDQIEERLKLLRKEMDLLRGNVLKA 1200

Query: 1202 ESVIKAAKKICNDESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDV 1261
            ESVIK AKK  NDES+KLDELQS+FKAAD+IRQEAYA+LQSTRKQL +KNKY W YR D 
Sbjct: 1201 ESVIKVAKKKYNDESIKLDELQSQFKAADEIRQEAYADLQSTRKQLSEKNKYCWNYRKDA 1260

Query: 1262 KEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSL 1321
            KEANEIA SGD+ERLQR  VNQVE MMELWNTNAEFREEYIKSNMRSTL RLKTLDGRSL
Sbjct: 1261 KEANEIALSGDLERLQRLCVNQVERMMELWNTNAEFREEYIKSNMRSTLWRLKTLDGRSL 1320

Query: 1322 GPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPA---ENMRDNDKPVIEVVKTKNQ 1381
            GPNEEPH  N+IVKERPA+D+SL TVS   E E LIPA   ++ RDNDK V +V +TKN+
Sbjct: 1321 GPNEEPHVPNRIVKERPAKDNSLLTVSTMQETEKLIPAADADDARDNDKSVTKVAETKNR 1380

Query: 1382 PTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKL 1441
             TK KKP TVVAL +G RNIS EN+VEEPP+PVEIKRTREEEE+AAKAEELRK+EEAIKL
Sbjct: 1381 TTK-KKPETVVALESGPRNISSENEVEEPPRPVEIKRTREEEEMAAKAEELRKEEEAIKL 1440

Query: 1442 KEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAE 1501
            KE+ KLEEK KAKEALERK+RNAEKA ARA  KARKEAEEREKLREKRAKKK RK+AA  
Sbjct: 1441 KERRKLEEKAKAKEALERKRRNAEKAQARAAIKARKEAEEREKLREKRAKKKERKIAAG- 1494

Query: 1502 TEAGNGLEEKESGIITEST----PKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIP 1561
            TEAGNG +E E  I+T++     PKE SEN G QGTAAKR PK + SQYTK SKTKS +P
Sbjct: 1501 TEAGNGRDEMECAIVTKTPAAEGPKEESENRGNQGTAAKRRPKKA-SQYTKQSKTKS-MP 1494

Query: 1562 PPLRNRSKRGMQPWMWVLLTTLIVFVVFFVG 1571
            PPLRNR KR MQPWMW+LLTTL+V  +FFVG
Sbjct: 1561 PPLRNRGKRRMQPWMWILLTTLVVLALFFVG 1494

BLAST of Cp4.1LG02g08540 vs. ExPASy TrEMBL
Match: A0A6J1IIW2 (uncharacterized protein LOC111474484 OS=Cucurbita maxima OX=3661 GN=LOC111474484 PE=4 SV=1)

HSP 1 Score: 1827 bits (4732), Expect = 0.0
Identity = 1081/1591 (67.94%), Postives = 1213/1591 (76.24%), Query Frame = 0

Query: 2    MADMDVHADVSQVYEKCALDMSTYENLPNACNAASLDSAASIPKDDTDASYVFVNSTDAA 61
            MADMDV AD S+V EKC LDMSTYENLP ACNA  LD A +IPKDDTD SYVFV+S DAA
Sbjct: 1    MADMDVLADFSEVNEKCTLDMSTYENLPKACNAEPLDCATAIPKDDTDGSYVFVSSNDAA 60

Query: 62   TSDDHVVSDLNGQLECSHNVDTEIKVHNGEFSTDDGRKPDSFFVSNASEDSPFKFSEHLK 121
            TSDDHV SD+NGQ +CS NVDTEIKV +GE STDD RKP+S FVS+A  DS F FS+H+ 
Sbjct: 61   TSDDHVASDVNGQAKCSQNVDTEIKVRDGELSTDDDRKPESIFVSDALGDSQFNFSDHVN 120

Query: 122  VSDAIQDETRVVDVLQSSDAKVDEPEPGLDSSQKVEETRILENQAVNESFDV---EGEIH 181
             SDAI DE RV DVLQSSDAK DE EP L+ S  VEETRI E QA NE FD     GEIH
Sbjct: 121  KSDAILDEIRVADVLQSSDAKEDEAEPQLNFSPTVEETRIPEGQATNEPFDAVTNSGEIH 180

Query: 182  GVDAPQEDENVQIQEENQIISTSESSNTDANLGGQILVVSSQMAEDIQIHEDNGVMGIMK 241
            GV++P E E++ IQEENQIIST  SSN D+N  G++ V SSQMAEDIQIHEDNG++GIMK
Sbjct: 181  GVESPHETEDIHIQEENQIISTPGSSNPDSNRSGEVKVESSQMAEDIQIHEDNGIVGIMK 240

Query: 242  SSNTKANRGIDIEAESSQNAEGIQFRKEYGIVAIKFSDPESNTGEEIEVESSLKADEVQN 301
            SS+T+ N  IDIEAESSQ A+ IQ  KE G VAI  SD ESN GEE EVESSLKAD+ QN
Sbjct: 241  SSDTEENPVIDIEAESSQKADSIQIHKENGTVAIMLSDTESNPGEETEVESSLKADDTQN 300

Query: 302  HEENGRVKAFNLTDTAANLRGKIKLESSKKADDIQNDEENGRMKAFDLSGTVANPRGKIE 361
            HEENG VKAF+                                                 
Sbjct: 301  HEENGIVKAFD------------------------------------------------- 360

Query: 362  VESSQKGDDILNDEENGRVKAFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVES 421
                                                        LSNTEANPRSE+E ES
Sbjct: 361  --------------------------------------------LSNTEANPRSELEEES 420

Query: 422  PGEGEDIKLNGQNEIVDAVKSSATLDKRGQ-GEAVPEDNETVAINELSDTIENRSEETEL 481
              E EDI+L GQNEIVD   SSA+++KRG+ GE +PEDNETV INELSDTI NRSEETE+
Sbjct: 421  SREVEDIELRGQNEIVDTNTSSASMEKRGEEGEVIPEDNETVVINELSDTIPNRSEETEM 480

Query: 482  DSFEREEGIRESQDANVEAADCH--SGKDKVDEMVNKAATSDPVGGLGESQIISMGAVKS 541
            +SFEREE IRESQDA +EAADCH  + ++KVDEMV+KA  SD VGG+GESQIIS+GA KS
Sbjct: 481  ESFEREESIRESQDATMEAADCHCVNVQEKVDEMVSKAVVSDSVGGIGESQIISLGAAKS 540

Query: 542  ELDHPDGSVEDVKGECKLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEP 601
            E+DH D SVEDVKGECK GVALNEEN E TQ+TI+ DGE   V GEEQESLNNE SLLEP
Sbjct: 541  EVDHLDDSVEDVKGECKQGVALNEENPESTQITISQDGERFQVAGEEQESLNNELSLLEP 600

Query: 602  SEENKVDMEQHLAAAPSPLVNSEDVNGSISTST----ATSMDQDDPLKTIDDKDTVANTS 661
            SEENK DMEQ L A PSPLV  ED+NGS+   T     TSMDQDDPL+ IDDKDT+ N +
Sbjct: 601  SEENKADMEQDLEATPSPLVCPEDINGSMPIRTDDGLPTSMDQDDPLEAIDDKDTIVNRT 660

Query: 662  SFHDHTETLSSSVDPDIDSVETHKLTHTMLINDPKVELNEITVNEQEVNHVLELEETSET 721
            SFHD TE+   SVD DI + ETH L+ TM I+DP+VEL+EITVNEQ VNHV ELEE SET
Sbjct: 661  SFHDLTESSPGSVDYDIATDETHILSPTMFISDPRVELSEITVNEQVVNHVFELEENSET 720

Query: 722  ASHPKVDECDRVEVLEGTVSG--DEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVET 781
             S PKVDEC +V  LE TVSG  D+M  ALD+SR + GD+SVAGSQLIP ++E  +S+ET
Sbjct: 721  VSQPKVDECIKVGDLECTVSGNEDDMPTALDQSRIACGDNSVAGSQLIPEDIERVESIET 780

Query: 782  AVSSVVIGNTSVEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDYVQLDNEVRENH 841
            AVS VVIGNT +EIRE    + L+DP LR DL  E  TMSENVASAGD V  D EV EN 
Sbjct: 781  AVSIVVIGNTKIEIRETPSVNCLNDPFLRSDLHVEHYTMSENVASAGDDVLPDQEVSENR 840

Query: 842  EISLLGDNNFETKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNK 901
            E  LLGD+NFE   E+G IE+++QSTF  NDMRS+S D  SIE EERGSTVPEV NG  K
Sbjct: 841  EDDLLGDSNFEIMCENGHIEKDDQSTFHSNDMRSKSKDCTSIESEERGSTVPEVFNGVVK 900

Query: 902  STAIQQSSAVETDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCT-SETEV 961
            S AI QSSAVETDSE  DN+ SSS TANE S ++IEI SS+GGG R I  DDC+ S+TEV
Sbjct: 901  SPAIPQSSAVETDSESHDNKSSSSLTANENSVDDIEITSSMGGGSRTIPGDDCSMSKTEV 960

Query: 962  -KGSAVNDEVDLNPMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVG 1021
             K S + DE +LN +SD+  +TD K TK+ETEV+HE CQNEPSP+SPEGS +AL G+NVG
Sbjct: 961  LKRSMIKDEGNLNSISDVVFETDGKLTKDETEVIHEDCQNEPSPVSPEGSADALTGQNVG 1020

Query: 1022 TEAGTKPLNFLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDD 1081
             EAGT+P NFLV+VP+F D NIREQIKCAQTEVD+ TKDRDAIRVQIQ MRAA K LSD+
Sbjct: 1021 AEAGTRPFNFLVKVPRFDDQNIREQIKCAQTEVDRKTKDRDAIRVQIQTMRAACKALSDN 1080

Query: 1082 LEAAVSEGRAARDLLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLK 1141
            LEAA+SEGRAARDLL+ KR EIDSVQSVITKVKNAMS+ DIDGR+R+ EH+IEHETLPLK
Sbjct: 1081 LEAAMSEGRAARDLLKSKRLEIDSVQSVITKVKNAMSVEDIDGRIRNTEHMIEHETLPLK 1140

Query: 1142 EEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKA 1201
            EEKQL+REIKQLKQ REQLSST GKQ+ELQQALDQ+DQ EERLKLLRKEMDLLRGNVLKA
Sbjct: 1141 EEKQLLREIKQLKQQREQLSSTMGKQEELQQALDQRDQIEERLKLLRKEMDLLRGNVLKA 1200

Query: 1202 ESVIKAAKKICNDESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDV 1261
            ESVIK AKK  NDES+KLDELQS+FKAAD+IRQEAYANLQSTRKQL +KNKY W YR D 
Sbjct: 1201 ESVIKVAKKKYNDESIKLDELQSQFKAADEIRQEAYANLQSTRKQLSEKNKYCWNYRKDA 1260

Query: 1262 KEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSL 1321
            KEANEIA SGD+ERLQR  VNQVE MMELWNTNAEFREEYIKSNMRSTL RLKTLDGRSL
Sbjct: 1261 KEANEIALSGDLERLQRLCVNQVERMMELWNTNAEFREEYIKSNMRSTLWRLKTLDGRSL 1320

Query: 1322 GPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPA---ENMRDNDKPVIEVVKTKNQ 1381
            GPNEEPH  N IVKERPA+D+SL TVS   E E LIPA   ++ RDNDK V +V +TKN+
Sbjct: 1321 GPNEEPHVPNHIVKERPAKDNSLLTVSTMQETEKLIPAADADDARDNDKSVTKVAETKNR 1380

Query: 1382 PTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAIKL 1441
             TK KKP TVVAL +G R+IS EN+VEEPP+PVEIKRTREEEELAAKAEELRK+EEAIKL
Sbjct: 1381 TTK-KKPETVVALESGPRSISSENEVEEPPRPVEIKRTREEEELAAKAEELRKEEEAIKL 1440

Query: 1442 KEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKARKMAAAE 1501
            KE+ KLEEK KAKEALERK+RNAEKA ARA  K RKEAEEREKLREKRAKKK RK+AA  
Sbjct: 1441 KERRKLEEKAKAKEALERKRRNAEKAQARAAIKERKEAEEREKLREKRAKKKERKLAAG- 1494

Query: 1502 TEAGNGLEEKESGIITEST----PKEVSENTGKQGTAAKRPPKASQSQYTKHSKTKSTIP 1561
            TEAGNG +E E  I+T++     PKE SEN GKQGTAAKR PK + SQYTK SKTKS +P
Sbjct: 1501 TEAGNGRDEMECAIVTKTPAAEGPKEESENRGKQGTAAKRRPKKA-SQYTKQSKTKS-MP 1494

Query: 1562 PPLRNRSKRGMQPWMWVLLTTLIVFVVFFVG 1571
            PPLRNR KR MQPWMW+LLTTL+V  +FFVG
Sbjct: 1561 PPLRNRGKRRMQPWMWILLTTLVVLALFFVG 1494

BLAST of Cp4.1LG02g08540 vs. TAIR 10
Match: AT1G20970.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: guard cell, cultured cell; BEST Arabidopsis thaliana protein match is: proton pump interactor 1 (TAIR:AT4G27500.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 374.0 bits (959), Expect = 5.9e-103
Identity = 390/1299 (30.02%), Postives = 633/1299 (48.73%), Query Frame = 0

Query: 321  IKLESSKKADD--IQNDEENGRMKAFDLSGTVANPRGKIEVESSQKGDDILNDEENGRVK 380
            +K+++   ADD  +   +++  ++  +L  T   P   +E+  S+  D +          
Sbjct: 95   VKVDAPSIADDDVLGVSQDSQTLEKSELESTDDGPEEVVEIPKSEVEDSL---------- 154

Query: 381  AFNLSDTEVDIQNHKENGIMKAPKLSNTEANPRSEIEVESPGEGEDIKLNGQNEIVDAVK 440
                 +  VD Q H  NG +++      E+    E   +S    +D+  N   E    ++
Sbjct: 155  -----EKSVD-QQHPGNGHLESGLEGKVESKEEVEQLHDSEVGSKDLTKNNVEEPEVEIE 214

Query: 441  SSATLDKRG-QGEAVPEDNETVAINELSDTIE--NRSEETELDSFE-REEGIRESQDANV 500
            S +  D  G QG+ +    ++    ++S  ++     E+  +DS E RE  +  ++ +  
Sbjct: 215  SDSETDVEGHQGDKIEAQEKSDRDLDVSQDLKLNENVEKHPVDSDEVRESELVSAKVSPT 274

Query: 501  EAAD--CHSGKDKVDEMVNKAATSDPVGG-LGESQIISMGAVKSELDHPDGSVEDVKGEC 560
            E +D     G+  V +       S+ V   +G   +  +  V  E  HP    E ++   
Sbjct: 275  EPSDGGMDLGQPTVTDPAETINGSESVNDHVGSEPVTVLEPVSVENGHPPVESE-LERSS 334

Query: 561  KLGVALNEENSERTQVTINPDGEHCHVVGEEQESLNNEDSLLEPSEENKVDMEQHLAAAP 620
             +      E    +   + PD     VV  E  S    D   E    N + ++   +   
Sbjct: 335  DVPFTSVAEKVNASDGEVLPDSGTVDVVVSEVSS----DVPAETQALNAISLDSQPSGKD 394

Query: 621  SPLVN----SEDVNGSISTSTATSMD---QDDPLKTIDDKDTVANTSSFHDHTETLSSSV 680
            S + N    SE  +  + +      D    D  + T  +    ++ +      + +SS V
Sbjct: 395  SVVENGNSKSESEDSKMQSEIGAVDDGSVSDGSINTHPESQDASDPTCDQGGKQHISSEV 454

Query: 681  DPDIDSVETHKLTHTMLI---NDPKVELNE-ITVNEQEVNHVLEL----EETSETASHPK 740
               +D+  + +++  +++   N  +  +++ ++   Q+ +   E+    E+    A H  
Sbjct: 455  KEVLDAPASEEISDAVIVAKDNGSEAAISDGLSCTNQQGSESDEISGLVEKLPSHALHEV 514

Query: 741  VDECDRVEVLEGTVSGDEMSIALDESRTSYGDDSVAGSQLIPVEVEPAQSVETAVSSVVI 800
            V   +   V+   VS D  S  L E      + ++       +E     +V+ A +  V 
Sbjct: 515  VSSANDTSVI---VSDDTKSQGLSEDHGVDTNQTIQDDCSAELEEVTDVNVKHAPNEKVQ 574

Query: 801  GNTSVEIREMSCTHGLDDPVLRPDLEAEDCTMSENVASAGDY-VQLDNEVRENHEISLLG 860
            G+ S     +     L+      +L   D + + +  SA      +D  +      + + 
Sbjct: 575  GDNSEGNLNVGGDVCLNSAEEAKELPTGDLSGNASHESAETLSTNIDEPLSLLDTKTAVS 634

Query: 861  DNNFETKSESGDIEEENQSTFPCNDMRSESNDFISIECEERGSTVPEVPNGDNKSTAIQQ 920
            D    +   +G+I+     +     ++  +   ++    E G    EV  G   +     
Sbjct: 635  DFAESSAGVAGEIDAVAMESEAAQSIKQCAEAHVAPSIIEDGEIDREVNCGSEVNVTKTT 694

Query: 921  SSAVETDSEFRDNERSSSPTANEKSGENIEIASSVGGGGRDITSDDCTSETEVKGSAVND 980
              AV  D   ++          E+S  N +   +      +I +  C  + E K     D
Sbjct: 695  PVAVREDIPPKEVSEMEESDVKERSSINTDEEVATASVASEIKT--CAQDLESKVVTSTD 754

Query: 981  EVDLNPMSDIASQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPL 1040
             +       +    DS+P + +   V +   ++ +       ++A  G  V  E   +P 
Sbjct: 755  TIHTGAKDCV----DSQPAENKEGNVVDRTDDKVASTGEVSVLDASEGLTVAAEIEKRPF 814

Query: 1041 NFLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEG 1100
             FL RVP++ D  + EQ+K A+ +VDQ T++RDA+R  IQ +RA  K      +A ++E 
Sbjct: 815  YFLPRVPRYDDEKLAEQLKHAEEQVDQKTQNRDALRADIQKIRAICKDYDISYKAVMAEE 874

Query: 1101 RAARDLLRFKRQEIDSVQSVITKVKNAMSIGDIDGRVRSIEHIIEHETLPLKEEKQLIRE 1160
            R+AR  +  KRQEI+++QS+I++VK+A S+ DID RVR++EH ++H TL L EEK  +RE
Sbjct: 875  RSARKAMHSKRQEIEALQSMISRVKSAASVDDIDSRVRNMEHTMQHTTLSLNEEKGFMRE 934

Query: 1161 IKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAK 1220
            IKQLKQLREQ+SS+ G +DE++QALD+K++ EERLK+LRKE+D LR ++ KAE + KAAK
Sbjct: 935  IKQLKQLREQISSSMGTKDEVKQALDEKEKTEERLKVLRKELDALRNDLSKAEEITKAAK 994

Query: 1221 KICNDESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANEIAT 1280
            K C+ E     +LQ +F+AAD +RQEA+ +LQ  +KQ  +KNKY++KYRD+ + A+E+A 
Sbjct: 995  KKCDGEWEAQSKLQEQFRAADAVRQEAFVHLQDLKKQQREKNKYFFKYRDNSRAASEMAL 1054

Query: 1281 SGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEEPHA 1340
              D   LQ    +QVE  M +WN + EFR+ Y+KSN RST RRL TLDGRSLGP+EEP  
Sbjct: 1055 KKDRAALQSLCSDQVENFMNMWNNDDEFRKYYVKSNTRSTFRRLGTLDGRSLGPDEEPPR 1114

Query: 1341 LNQIVKERPARDSSLSTVSKTLEPENLIPAE----------NMRDNDKPVIEVVKTKNQP 1400
            +         R   L T S   E    +PA+           + +N K V +  + K+Q 
Sbjct: 1115 ITY-----APRTDKLRTSSDRAEKHEAVPAQKEKVVKFEGSKVENNGKEVAKPTEQKSQT 1174

Query: 1401 TKNKK-------PTTVVALMNGLRNI--SCENDVEEPPQPVEIKRTREEEELAAKAEELR 1460
            TK+KK       P+ V  L++G   I  S   + EEPP     K T+EEEEL  K EE R
Sbjct: 1175 TKSKKAVKPDQPPSIVTELVSGKEEIEKSATPEEEEPP-----KLTKEEEELIKKEEEKR 1234

Query: 1461 KKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKK 1520
            K++EA K+KEQH+LEE  KAKEA+ERKK+  EKA ARAV KA+KEAEEREK REK+ +KK
Sbjct: 1235 KQKEAAKMKEQHRLEEIAKAKEAMERKKKREEKAKARAVLKAQKEAEEREKEREKKLRKK 1294

Query: 1521 ARK---MAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRPPKASQSQYTKHS 1572
             R+     + ET   N +   E+ + T    +   + T ++    K+  K S SQ+ K +
Sbjct: 1295 ERRKGIFTSEETATENPIPTAETVVETPREIETPKKQTVEESQQMKKSHKPS-SQFLKQN 1351

BLAST of Cp4.1LG02g08540 vs. TAIR 10
Match: AT4G27500.1 (proton pump interactor 1 )

HSP 1 Score: 130.2 bits (326), Expect = 1.5e-29
Identity = 142/577 (24.61%), Postives = 278/577 (48.18%), Query Frame = 0

Query: 1017 FLVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGR 1076
            +LV+   + D  I+ ++  A  E+++  K R  +  +++  RA    L D L+   SE +
Sbjct: 80   YLVKYRSYADPKIKAKLDLADKELEKLNKARTGVLDKLRAKRAERSELFDLLDPLKSERK 139

Query: 1077 AARDLLRFKRQEIDSVQSVITKVKN-----------AMSIGDIDGRVRSIEHIIEHETLP 1136
                +   KR+E++ +Q  + K+++             S  +++  + S ++ I+HE++P
Sbjct: 140  GFNTMFDEKRKEMEPLQQALGKLRSNDGGSARGPAICSSEEELNSMIYSYQYRIQHESIP 199

Query: 1137 LKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLL-------RKEMD 1196
            L EEKQ+++EI+ L+  R+++ +    + ++++++ QKD  + ++KL+       +KE  
Sbjct: 200  LTEEKQILKEIRLLEGTRDKVIANAAMRAKIKESMGQKDDIQGQVKLMGAGLDGVKKERQ 259

Query: 1197 LLRGNVLKAESVIKAAKKICNDESLKLDELQSKFKAADKIRQEAYANLQSTRKQLYDKNK 1256
             +   + +    +KA K        ++  L+++ K   + R +AY+N+   R+Q  + N 
Sbjct: 260  AISARINELSEKLKATKD-------EITVLENELKTVSEKRDKAYSNIHDLRRQRDETNS 319

Query: 1257 YYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRR 1316
             Y++ R  + +A ++A   ++  L+  +  +VE  + LW +   FRE+Y K  ++S   R
Sbjct: 320  EYYQNRTVLNKARDLAAQKNISELEALANAEVEKFISLWCSKKNFREDYEKRILQSLDSR 379

Query: 1317 LKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEV 1376
              + DGR   P+E+P          P    S +T S+T     ++P    +   KP    
Sbjct: 380  QLSRDGRMRNPDEKPLI-------APEAAPSKATPSET----EVVP----KAKAKP---- 439

Query: 1377 VKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEE----LAAKAEE 1436
             + K +P    KP   VA            + E+    V++K   ++++       K ++
Sbjct: 440  -QPKEEPVSAPKPDATVA-----------QNTEKAKDAVKVKNVADDDDDEVYGLGKPQK 499

Query: 1437 LRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAK 1496
              K  +A   KE  K EE  KAK+A+ERKK+ AEKA A+A  +A+KEAE++EK   K  +
Sbjct: 500  EEKPVDAATAKEMRKQEEIAKAKQAMERKKKLAEKAAAKAAIRAQKEAEKKEK---KEQE 559

Query: 1497 KKARKMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRPPKASQSQYTKHSK 1556
            KKA+K     TE        E+  + E++ +E+     ++    ++  K    +     +
Sbjct: 560  KKAKKKTGGNTET-------ETEEVPEASEEEIEAPVQEEKPQKEKVFKEKPIRNRTRGR 607

Query: 1557 TKSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFVG 1572
               TIP  +  R K+    W++     L+V ++  +G
Sbjct: 620  GPETIPRAILKR-KKSTNYWVYAAPAALVVLLLLVLG 607

BLAST of Cp4.1LG02g08540 vs. TAIR 10
Match: AT3G15340.1 (proton pump interactor 2 )

HSP 1 Score: 75.9 bits (185), Expect = 3.3e-13
Identity = 136/575 (23.65%), Postives = 253/575 (44.00%), Query Frame = 0

Query: 1013 KPLNF-LVRVPKFGDVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAA 1072
            KP +F  V+ P + D  I+ +I  A  E+++  KDR  +    +  +A    L   +++ 
Sbjct: 65   KPFSFYFVKQPAYDDPEIKAKIDEAGHEINRYNKDRIVVSNAQESEKAEILSLFGQMKSL 124

Query: 1073 VSEGRAARDLLRFKRQEIDSVQSVI------TKVKNAMSIGDIDGRVRSIEHIIEHETLP 1132
            VS+    R ++  K+ E D++   +      T  +   S  ++D  +    + IEH ++ 
Sbjct: 125  VSKSEGYRVVIEEKKMEFDALHESLRNLRCSTSDQLCFSKEELDHLIYIAHYQIEHGSIG 184

Query: 1133 LKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVL 1192
             +EE  +++E ++   +     S   K+  + +      +  E    ++KE+D +   + 
Sbjct: 185  FEEEDWVLKETEKADGIVLSEDSLAEKEASINRVKSMAVELNE----VKKELDAITWKI- 244

Query: 1193 KAESVIKAAKKICNDESLKLDELQSKFKAAD-------KIRQEAYANLQSTRKQLYDKNK 1252
                         N  S K+ + Q+  +  D       + R  +Y  ++  R Q      
Sbjct: 245  -------------NHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRDKGKA 304

Query: 1253 YYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRR 1312
             +++    +++A E+A SG+V  L+ F+ ++ +  M  WN +  FR++Y+K    S   R
Sbjct: 305  AFYQSLPVMRKARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPSLCER 364

Query: 1313 LKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEV 1372
                DGR   P        Q+V E+         V +T         E+   N      V
Sbjct: 365  QLNQDGRIKDPEV------QVVWEKKVPVKGGEKVHET-------NREDSSSNSSQYGSV 424

Query: 1373 VKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKK 1432
            +  K +    KK       M+  R+ + E+DV +    V  K  +EEEE+          
Sbjct: 425  ITDKRKKETRKK------AMDFNRSSAEESDVTDLEFSVYEKPKKEEEEV---------D 484

Query: 1433 EEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAKKKAR 1492
            EE   LKE+ + E+  KA+ A+ERK++  EKA A+A  +A+KEAE++ K  EK+AKKKA 
Sbjct: 485  EET--LKEREREEQLEKARLAMERKRKLQEKAAAKAAIRAQKEAEKKLKECEKKAKKKAA 544

Query: 1493 KMAAAETEAGNGLEEKESGIITESTPK-EVSENTGKQGTAAKRP--PKASQSQYTKHSKT 1552
              +++ +E+ +  E      +T+   K      +GK+    +R   PK    +Y    + 
Sbjct: 545  ANSSSPSESDHSQE------VTKDLEKVRTLAVSGKEKHQKERSLFPKQRSFRYKHRGRG 584

Query: 1553 KSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFV 1571
               +P  + NR ++  + W+W L +  +   +F V
Sbjct: 605  TEALPKAILNR-RKAHKYWVWGLSSAALAVALFLV 584

BLAST of Cp4.1LG02g08540 vs. TAIR 10
Match: AT3G15340.2 (proton pump interactor 2 )

HSP 1 Score: 60.1 bits (144), Expect = 1.9e-08
Identity = 121/519 (23.31%), Postives = 227/519 (43.74%), Query Frame = 0

Query: 1068 LEAAVSEGRAARDLLRFKRQEIDSVQSVI------TKVKNAMSIGDIDGRVRSIEHIIEH 1127
            +++ VS+    R ++  K+ E D++   +      T  +   S  ++D  +    + IEH
Sbjct: 1    MKSLVSKSEGYRVVIEEKKMEFDALHESLRNLRCSTSDQLCFSKEELDHLIYIAHYQIEH 60

Query: 1128 ETLPLKEEKQLIREIKQLKQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLR 1187
             ++  +EE  +++E ++   +     S   K+  + +      +  E    ++KE+D + 
Sbjct: 61   GSIGFEEEDWVLKETEKADGIVLSEDSLAEKEASINRVKSMAVELNE----VKKELDAIT 120

Query: 1188 GNVLKAESVIKAAKKICNDESLKLDELQSKFKAAD-------KIRQEAYANLQSTRKQLY 1247
              +              N  S K+ + Q+  +  D       + R  +Y  ++  R Q  
Sbjct: 121  WKI--------------NHLSDKVGKSQNNLRVLDVKKAHILEERDRSYERIKMLRIQRD 180

Query: 1248 DKNKYYWKYRDDVKEANEIATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRS 1307
                 +++    +++A E+A SG+V  L+ F+ ++ +  M  WN +  FR++Y+K    S
Sbjct: 181  KGKAAFYQSLPVMRKARELAASGNVRDLEVFASSEADRFMTQWNDDKAFRDDYVKRISPS 240

Query: 1308 TLRRLKTLDGRSLGPNEEPHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKP 1367
               R    DGR   P        Q+V E+         V +T         E+   N   
Sbjct: 241  LCERQLNQDGRIKDPEV------QVVWEKKVPVKGGEKVHET-------NREDSSSNSSQ 300

Query: 1368 VIEVVKTKNQPTKNKKPTTVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEE 1427
               V+  K +    KK       M+  R+ + E+DV +    V  K  +EEEE+      
Sbjct: 301  YGSVITDKRKKETRKK------AMDFNRSSAEESDVTDLEFSVYEKPKKEEEEV------ 360

Query: 1428 LRKKEEAIKLKEQHKLEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKLREKRAK 1487
                EE   LKE+ + E+  KA+ A+ERK++  EKA A+A  +A+KEAE++ K  EK+AK
Sbjct: 361  ---DEET--LKEREREEQLEKARLAMERKRKLQEKAAAKAAIRAQKEAEKKLKECEKKAK 420

Query: 1488 KKARKMAAAETEAGNGLEEKESGIITESTPK-EVSENTGKQGTAAKRP--PKASQSQYTK 1547
            KKA   +++ +E+ +  E      +T+   K      +GK+    +R   PK    +Y  
Sbjct: 421  KKAAANSSSPSESDHSQE------VTKDLEKVRTLAVSGKEKHQKERSLFPKQRSFRYKH 464

Query: 1548 HSKTKSTIPPPLRNRSKRGMQPWMWVLLTTLIVFVVFFV 1571
              +    +P  + NR ++  + W+W L +  +   +F V
Sbjct: 481  RGRGTEALPKAILNR-RKAHKYWVWGLSSAALAVALFLV 464

BLAST of Cp4.1LG02g08540 vs. TAIR 10
Match: AT5G36780.1 (AT5G36780 and AT5G36690 represent identical copies. The duplication within clone F27C7 is believed an artifact with only one true copy existing. )

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-06
Identity = 150/638 (23.51%), Postives = 263/638 (41.22%), Query Frame = 0

Query: 967  SQTDSKPTKEETEVVHESCQNEPSPISPEGSIEALAGKNVGTEAGTKPLNF-LVRVPKFG 1026
            S T+S+ T +E E    S  +  S ++ E              +G KPL+F +V+ P + 
Sbjct: 30   SLTNSEVTTDEEEDTIFSGGDSSSGLAAEED-----------SSGDKPLSFYIVKQPVYD 89

Query: 1027 DVNIREQIKCAQTEVDQTTKDRDAIRVQIQIMRAAWKVLSDDLEAAVSEGRAARDLLRFK 1086
            +  I+ +I  A  E+ +  + R  +    +   A    L   +E+ V +    R ++  K
Sbjct: 90   NPEIKAKIDEANQEIFRCNELRINVLSAKKSELAEVSSLYTQMESLVPQSEGYRMVIEEK 149

Query: 1087 RQEIDSVQSVITKVKNAMS------IGDIDGRVRSIEHIIEHETLPLKEEKQLIREIKQL 1146
            ++E D++   +  ++   S        ++D      ++ IE+ ++ L+EE  +++E    
Sbjct: 150  KKEFDTLLEALRNLRCTTSDQLCFTKEELDHLSYIAQYQIEYGSIGLEEEDWMLKE---- 209

Query: 1147 KQLREQLSSTTGKQDELQQALDQKDQFEERLKLLRKEMDLLRGNVLKAESVIKAAKKICN 1206
                      T K D +   L +K+    R+K +  E++       + ++ + A     N
Sbjct: 210  ----------TEKPDGI--ILSEKEASINRVKSMALELN-------EVKNELDAITWKIN 269

Query: 1207 DESLKLDELQSKFKAAD-------KIRQEAYANLQSTRKQLYDKNKYYWKYRDDVKEANE 1266
            D S KL + Q+  +  D       + R   YA ++  R Q    N  +++    + +A E
Sbjct: 270  DLSDKLWKSQNNIRVLDLEKAHILEKRDRFYARIKMLRIQRDKGNAAFFQSLSVMCKAKE 329

Query: 1267 IATSGDVERLQRFSVNQVECMMELWNTNAEFREEYIKSNMRSTLRRLKTLDGRSLGPNEE 1326
            +A SG+V  L+ F+ ++V+  M LWN +  FRE+Y++    S   R    DGR       
Sbjct: 330  LAASGNVRELEVFASSEVDRFMTLWNDDKAFREDYVRRISHSLCERELNEDGR------- 389

Query: 1327 PHALNQIVKERPARDSSLSTVSKTLEPENLIPAENMRDNDKPVIEVVKTKNQPTKNKKPT 1386
                                               ++D D   +++   K  P K  K +
Sbjct: 390  -----------------------------------IKDAD---LQIFWEKKVPVKTIKRS 449

Query: 1387 TVVALMNGLRNISCENDVEEPPQPVEIKRTREEEELAAKAEELRKKEEAI---KLKEQHK 1446
              V  MN  R  S  N  E+    +  KR +E +      E+ +KKEE I    LKE+ +
Sbjct: 450  EKVHKMN--REDSSSNSSED-GNVITDKRKKETKSDVIVYEKPKKKEEEIDEEALKERKR 509

Query: 1447 LEEKTKAKEALERKKRNAEKAHARAVTKARKEAEEREKL-----------REKRAKKKAR 1506
             E+  KA+  +ERK++  EKA A+A  +A+KEAE++ K             EK+AKKKA 
Sbjct: 510  EEQLEKARLVMERKRKLQEKAAAKAAIRAQKEAEKKLKAIILSCSHFFNECEKKAKKKA- 569

Query: 1507 KMAAAETEAGNGLEEKESGIITESTPKEVSENTGKQGTAAKRP--PKASQSQYTKHSKTK 1566
                    A N     ES  +           +GK+    +R   PK    +Y       
Sbjct: 570  --------AANSTSPSESDQVINDEKVRTLAVSGKEKHQKERLLFPKQRSFRYKHRGSGT 575

Query: 1567 STIPPPLRNRSKRGMQPWMWVL----LTTLIVFVVFFV 1571
              +P  + NR ++  + W+W L    L   +V VV  +
Sbjct: 630  EALPKAILNR-RKARRYWVWGLSSAALAVSLVLVVLLL 575