Cp4.1LG02g07300 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g07300
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCalcium uniporter protein
LocationCp4.1LG02: 721932 .. 723514 (+)
RNA-Seq ExpressionCp4.1LG02g07300
SyntenyCp4.1LG02g07300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAACTCAATCATCCAAACACTGCCCTAATTTTACGCTTCGATTTCCCTTCCCCCGATTTTCCCCCTCTGCTCCGTCAATGGCGCTCCGGAGATCCATTTCCAAGCGCTTTCTTGATGGTTTCAGAACTAATTCTTCTCCATCGCCTATCAATTTGACCTCCCGCCGCGACTACATTTCTCCTCCGCCCGATTCCGCCGGGAAGTCCGTTTTCAGGCGCTTTCTTCAGAGGAGAGCGATCTATCATTCTCCTTCTGCTGCTCGGATCCCCGAGTTGTTGACTCTTCCATTAGGCGATGATCTCAGAGAGAAACTCGGAGGTCGCAACATTAGTGCCGATCGAATCCGCCTCGACGGCCTCATTCCTCCGTCGTCGTTCGATAATGAACGCTCTGTTTCTGGATGTGGTATCTCGATCGACGATGCTAGGAAGATCATTAGGCTATCGCAGATCGAAAAACTAAAGGCGAAATTGAGAAATATTCAGAAGAGTTCGATCTCGTACTCCGAGTTCTTTCGGATCTGTGTTGAAGATTGTGGGAATAAGGATCAGGGCGCCGAATTCGCCAAGTTGTTGGATGAATCTGGAAGCGTCATTGTCATTGGAAATATCGTGTTCCTCCGGCCGGATCAGGTAAAATTATAAGTGCGATTAGGTGAAGATTTACAGATTAAAAAAAAAAAAAACTGACCGAATCCATCCCTTACGGAGATCCGATTTAAGCGGACGATGACGATGAACAGTGTTGCAAGAATATACAGAATCAAAATCATCACAAATTTGGAAACATGTTTTAAGTGGTTGAAATTTTGGGACAAAATCGTGACATATTCAACGAATAGTTAATTAATTAAACAAAAAATTAAAAAAGTTTAACAAAGAAAAAGGAAAATGTTGAAATCCTTCTGTGATTGACGAGTTTGAAATATGTACAGGTGGCTAAATCAATGGAAACGATGATATCTGGATCAATAGCGAGACCAGACGACCCAAGGTTGAAACAACTAGCCCAAATGGAGAGGCAAAAGGAAATAATCGACAAAAAGGCCAAGGCCCAGGTTCAAGGCGAGCTCTTTTGTGGATTGGGCCTTCTCGTGGCCCAAACATTGGGCTGCATGAGGCTCACTTTCTGGGAGCTAAGTTGGGACGTAATGGAGCCCATTTGCTTCTTCGTGACATCCCTTCACTTTGCCCTTGCCTATGCCTTCTTCCTTACAACCTCCACAGAGCCCACTTTTGAAGGCATCTTCCGACGTCGTTTTAAGGCAAAGCAAAAGAAACTCATGGCCTCTCAGAATTTCGACATCAACAACTACAATGAACTGAAGAGAATTTGCTATCCAAGCTACCCAACTCAACAAGAACCCTTGTTTTCCAATGCCCATACTCTATCATAAGACTTTTTTGTAAATTTAATAATTCCATTTTTTTTTTCTCTTCTAAAATCGGAGCTTTAGGATTCAGCTTTTTTTTTTCTTTTTTATTTGAGTGTCTTGATCTAAATCGGCTTTGGACATGATACATCGATTCGTATCATGTATTGTATAGGACCATTGAAAAAAAAAATTAATATAAAAACGAGA

mRNA sequence

CAAACTCAATCATCCAAACACTGCCCTAATTTTACGCTTCGATTTCCCTTCCCCCGATTTTCCCCCTCTGCTCCGTCAATGGCGCTCCGGAGATCCATTTCCAAGCGCTTTCTTGATGGTTTCAGAACTAATTCTTCTCCATCGCCTATCAATTTGACCTCCCGCCGCGACTACATTTCTCCTCCGCCCGATTCCGCCGGGAAGTCCGTTTTCAGGCGCTTTCTTCAGAGGAGAGCGATCTATCATTCTCCTTCTGCTGCTCGGATCCCCGAGTTGTTGACTCTTCCATTAGGCGATGATCTCAGAGAGAAACTCGGAGGTCGCAACATTAGTGCCGATCGAATCCGCCTCGACGGCCTCATTCCTCCGTCGTCGTTCGATAATGAACGCTCTGTTTCTGGATGTGGTATCTCGATCGACGATGCTAGGAAGATCATTAGGCTATCGCAGATCGAAAAACTAAAGGCGAAATTGAGAAATATTCAGAAGAGTTCGATCTCGTACTCCGAGTTCTTTCGGATCTGTGTTGAAGATTGTGGGAATAAGGATCAGGGCGCCGAATTCGCCAAGTTGTTGGATGAATCTGGAAGCGTCATTGTCATTGGAAATATCGTGTTCCTCCGGCCGGATCAGGTGGCTAAATCAATGGAAACGATGATATCTGGATCAATAGCGAGACCAGACGACCCAAGGTTGAAACAACTAGCCCAAATGGAGAGGCAAAAGGAAATAATCGACAAAAAGGCCAAGGCCCAGGTTCAAGGCGAGCTCTTTTGTGGATTGGGCCTTCTCGTGGCCCAAACATTGGGCTGCATGAGGCTCACTTTCTGGGAGCTAAGTTGGGACGTAATGGAGCCCATTTGCTTCTTCGTGACATCCCTTCACTTTGCCCTTGCCTATGCCTTCTTCCTTACAACCTCCACAGAGCCCACTTTTGAAGGCATCTTCCGACGTCGTTTTAAGGCAAAGCAAAAGAAACTCATGGCCTCTCAGAATTTCGACATCAACAACTACAATGAACTGAAGAGAATTTGCTATCCAAGCTACCCAACTCAACAAGAACCCTTGTTTTCCAATGCCCATACTCTATCATAAGACTTTTTTGTAAATTTAATAATTCCATTTTTTTTTTCTCTTCTAAAATCGGAGCTTTAGGATTCAGCTTTTTTTTTTCTTTTTTATTTGAGTGTCTTGATCTAAATCGGCTTTGGACATGATACATCGATTCGTATCATGTATTGTATAGGACCATTGAAAAAAAAAATTAATATAAAAACGAGA

Coding sequence (CDS)

ATGGCGCTCCGGAGATCCATTTCCAAGCGCTTTCTTGATGGTTTCAGAACTAATTCTTCTCCATCGCCTATCAATTTGACCTCCCGCCGCGACTACATTTCTCCTCCGCCCGATTCCGCCGGGAAGTCCGTTTTCAGGCGCTTTCTTCAGAGGAGAGCGATCTATCATTCTCCTTCTGCTGCTCGGATCCCCGAGTTGTTGACTCTTCCATTAGGCGATGATCTCAGAGAGAAACTCGGAGGTCGCAACATTAGTGCCGATCGAATCCGCCTCGACGGCCTCATTCCTCCGTCGTCGTTCGATAATGAACGCTCTGTTTCTGGATGTGGTATCTCGATCGACGATGCTAGGAAGATCATTAGGCTATCGCAGATCGAAAAACTAAAGGCGAAATTGAGAAATATTCAGAAGAGTTCGATCTCGTACTCCGAGTTCTTTCGGATCTGTGTTGAAGATTGTGGGAATAAGGATCAGGGCGCCGAATTCGCCAAGTTGTTGGATGAATCTGGAAGCGTCATTGTCATTGGAAATATCGTGTTCCTCCGGCCGGATCAGGTGGCTAAATCAATGGAAACGATGATATCTGGATCAATAGCGAGACCAGACGACCCAAGGTTGAAACAACTAGCCCAAATGGAGAGGCAAAAGGAAATAATCGACAAAAAGGCCAAGGCCCAGGTTCAAGGCGAGCTCTTTTGTGGATTGGGCCTTCTCGTGGCCCAAACATTGGGCTGCATGAGGCTCACTTTCTGGGAGCTAAGTTGGGACGTAATGGAGCCCATTTGCTTCTTCGTGACATCCCTTCACTTTGCCCTTGCCTATGCCTTCTTCCTTACAACCTCCACAGAGCCCACTTTTGAAGGCATCTTCCGACGTCGTTTTAAGGCAAAGCAAAAGAAACTCATGGCCTCTCAGAATTTCGACATCAACAACTACAATGAACTGAAGAGAATTTGCTATCCAAGCTACCCAACTCAACAAGAACCCTTGTTTTCCAATGCCCATACTCTATCATAA

Protein sequence

MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSAARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVFLRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKKLMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS
Homology
BLAST of Cp4.1LG02g07300 vs. ExPASy Swiss-Prot
Match: Q9FJV7 (Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g42610 PE=2 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 9.5e-72
Identity = 150/325 (46.15%), Postives = 205/325 (63.08%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRR+I+KR +D +R++ +                      S FR+         SP  
Sbjct: 1   MALRRAITKRLIDCYRSDVTQQ-------------------HSFFRQL--------SPYN 60

Query: 61  ARI-PELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKI 120
            +I  + +T P G +L +++ G       IR++ + PP            G+S+ + +K+
Sbjct: 61  MKIHRQYMTSPNGPNLTDRIVG-------IRIESVSPP----RREETPVMGLSVSETKKL 120

Query: 121 IRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIV 180
           +RL Q+EK+K +LR I KSS+SY EF +IC E CGN +QG++ AK LD SG V+V+G+IV
Sbjct: 121 LRLYQMEKVKERLREIPKSSVSYWEFIQICCESCGNDEQGSQMAKSLDHSGCVVVLGDIV 180

Query: 181 FLRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLV 240
           FL P Q+AKSME MI  +   P+DPR ++L Q+   K+ ID +A+  VQ EL+CGLG L 
Sbjct: 181 FLHPHQIAKSMEAMIKQTSVLPNDPRKEELVQLATTKKSIDIEARRIVQAELYCGLGFLA 240

Query: 241 AQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQK 300
            QT+G MRLTFWELSWDVMEPICFFVT++HF L Y FFL TSTEP+FEG+FR+RFK KQK
Sbjct: 241 VQTIGFMRLTFWELSWDVMEPICFFVTTIHFILGYIFFLRTSTEPSFEGLFRQRFKTKQK 286

Query: 301 KLMASQNFDINNYNELKRICYPSYP 325
           KLM    FD   YNEL  + + ++P
Sbjct: 301 KLMERHGFDFLRYNELNSL-FTTFP 286

BLAST of Cp4.1LG02g07300 vs. ExPASy Swiss-Prot
Match: O64823 (Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g23790 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.5e-64
Identity = 146/334 (43.71%), Postives = 208/334 (62.28%), Query Frame = 0

Query: 1   MALRRSISKRF----------LDGFRTNSSPSPI-----NLTSRRDYISPPPDSAGKSVF 60
           MA+R+ +SK+           L   R +SS   +     N ++    I+P P     S  
Sbjct: 1   MAMRKLLSKKLFNITNVASQSLMNCRISSSSLAVRTRVPNDSTDTTKIAPEPGDLAMS-- 60

Query: 61  RRFLQRRAIYHSPSAARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERS 120
           RRF+      H+ +  R  E++ +P+G+ L EKL   + S DRIRLDGL PP     ER 
Sbjct: 61  RRFM------HNSAMIRPAEIMQMPVGESLIEKLREIDGSKDRIRLDGLSPP-----ERE 120

Query: 121 VSGCGISIDDARKIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKL 180
            S   +++ D +K++R +QIE +K+KLR   +S + Y EF  +C E   + D G++ AK+
Sbjct: 121 TS---LTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVCGEASSDPDLGSKIAKM 180

Query: 181 LDESGSVIVIGNIVFLRPDQVAKSMETMIS-GSIARPDDPRLKQLAQMERQKEIIDKKAK 240
           LD+S +VIV+G+ V +RPDQV KS+E ++    I  P+DPR  +  ++E +K +ID KA 
Sbjct: 181 LDDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAH 240

Query: 241 AQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEP 300
             V+ EL+ GLG L+ QT G MRLTFWELSWDVMEPICF+VTS++F   YAFFL TS EP
Sbjct: 241 TLVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEP 300

Query: 301 TFEGIFRRRFKAKQKKLMASQNFDINNYNELKRI 319
           +FEG ++ RF+AKQ+KLM    FD+  YNELK++
Sbjct: 301 SFEGFYQSRFEAKQRKLMNEYEFDLERYNELKKL 318

BLAST of Cp4.1LG02g07300 vs. ExPASy Swiss-Prot
Match: Q1PE15 (Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g36820 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.4e-61
Identity = 123/286 (43.01%), Postives = 188/286 (65.73%), Query Frame = 0

Query: 37  PDSAGKSVFRRFLQRRAIYHSPSAARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIP 96
           P+    ++ +RFL + ++    + ++      + +G+ L EKL   +++ DRIRLDGL  
Sbjct: 51  PEPGDLTISQRFLNKFSMNGIDTTSK------MSIGESLMEKLKEMDMNKDRIRLDGLSH 110

Query: 97  PSSFDNERSVSGCGISIDDARKIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNK 156
           P            G+++ D +K++R ++IE +K KL    K  I YS+F  +C +   + 
Sbjct: 111 PKE-------ETLGLTVQDVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLDP 170

Query: 157 DQGAEFAKLLDESGSVIVIGNIVFLRPDQVAKSMETMIS-GSIARPDDPRLKQLAQMERQ 216
            QGA  AK+LD+SG+VIV+GN V LRP Q+ KS+E ++    I  P+DPR K+L ++E  
Sbjct: 171 SQGALIAKMLDDSGNVIVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAI 230

Query: 217 KEIIDKKAKAQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYA 276
           K +ID+KA + V+ EL+ GLG L+ QT G MRLTFW+L+WDVMEPICF+V+S++F   Y 
Sbjct: 231 KTVIDQKAHSLVRRELWAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYT 290

Query: 277 FFLTTSTEPTFEGIFRRRFKAKQKKLMASQNFDINNYNELKRICYP 322
           FFL TS EP+F+G ++ RF+AKQ+KLM S++FD+  Y+ELK++  P
Sbjct: 291 FFLKTSREPSFQGFYQSRFEAKQRKLMQSEDFDVGRYDELKKLFNP 323

BLAST of Cp4.1LG02g07300 vs. ExPASy Swiss-Prot
Match: Q9LVR5 (Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g66650 PE=2 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-58
Identity = 122/288 (42.36%), Postives = 186/288 (64.58%), Query Frame = 0

Query: 36  PPDSAGKSVFRRFLQRRAIYHSPSAARIPELLTLPLGDDLREKLGGRNIS-ADRIRLDGL 95
           PPD+    + RRFL +RA +        PE+  +P G +L EKL    +S  +RIRLD +
Sbjct: 36  PPDAGDSGIRRRFLHKRAFFS-------PEI--VPKGGNLMEKLRELTLSNNNRIRLDEM 95

Query: 96  IPPSSFDNERSVSGCGISIDDARKIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCG 155
           +PP S           ++++D +K++R +++E +K+KLR I K+ + YSEF R+C E   
Sbjct: 96  LPPPSPKKSSPEFFPAVTVEDVKKLMRAAEMELVKSKLREIGKNWVPYSEFVRVCGEYSS 155

Query: 156 NKDQGAEFAKLLDESGSVIVIGNIVFLRPDQVAKSMETMISGSIARPDDPRLKQLAQMER 215
           + +QG   A +LDE+G+VIV+G +V L+P+++  +M  +I       D    ++  Q+E 
Sbjct: 156 DPEQGNRVANMLDEAGNVIVLGKLVCLKPEELTSAMAGLIPTLEPSLDAETRQEFEQLEI 215

Query: 216 QKEIIDKKAKAQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAY 275
            K  IDK+A   V+ EL+ GLGL++AQT+G  RLTFWELSWDVMEPICF+VTS +F   Y
Sbjct: 216 IKSDIDKRADDLVRKELWAGLGLIMAQTVGFFRLTFWELSWDVMEPICFYVTSTYFMAGY 275

Query: 276 AFFLTTSTEPTFEGIFRRRFKAKQKKLMASQNFDINNYNELKRICYPS 323
           AFFL TS EP+FEG ++ RF+ KQK+L+   +FDI+ + +L+++  P+
Sbjct: 276 AFFLRTSKEPSFEGFYKSRFETKQKRLIKMLDFDIDRFTKLQKMHRPN 314

BLAST of Cp4.1LG02g07300 vs. ExPASy Swiss-Prot
Match: F4I111 (Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g09575 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.9e-29
Identity = 77/225 (34.22%), Postives = 125/225 (55.56%), Query Frame = 0

Query: 97  PSSFDNERSVSGCGISIDDARKIIRLSQIEKLKAKLRNI-QKSSISYSEFFRIC--VEDC 156
           PS  + E+      I+I +A+K++RL  +E +K KL  +  +  +SYS        +   
Sbjct: 39  PSEDEEEKKKE---ITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLEASQGMGIA 98

Query: 157 GNKDQGAEFAKLLDESGSVIVIGNIVFLRPDQVAKSMETMISGSIARPDDPRLKQLAQME 216
            + D+   FA++LD++G V++  + V+L PD+V   +   +       +D   ++  ++ 
Sbjct: 99  RSPDEAHVFARVLDDAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEEFNKLR 158

Query: 217 RQKEIIDKKAKAQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALA 276
             KE ID  A  QV+  L+CGL   + Q     RLTFWE SWDVMEPI FF T+    + 
Sbjct: 159 IMKEEIDVLAHRQVRKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATATGIIVG 218

Query: 277 YAFFLTTSTEPTFEGIFRRRFKAKQKKLMASQNFDINNYNELKRI 319
           YA+FL TS +PT++   +R F ++Q+KL+ S  FD   + EL+R+
Sbjct: 219 YAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDCERFKELERL 260

BLAST of Cp4.1LG02g07300 vs. NCBI nr
Match: XP_023523255.1 (calcium uniporter protein 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667 bits (1721), Expect = 1.24e-241
Identity = 338/338 (100.00%), Postives = 338/338 (100.00%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA
Sbjct: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
           ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII
Sbjct: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF
Sbjct: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA
Sbjct: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK
Sbjct: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300

Query: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338
           LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS
Sbjct: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338

BLAST of Cp4.1LG02g07300 vs. NCBI nr
Match: XP_022940100.1 (calcium uniporter protein 4, mitochondrial-like [Cucurbita moschata] >KAG6608518.1 Calcium uniporter protein 4, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037841.1 Calcium uniporter protein 4, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 665 bits (1716), Expect = 7.20e-241
Identity = 336/338 (99.41%), Postives = 337/338 (99.70%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA
Sbjct: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
           ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII
Sbjct: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQ+EKLK KLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF
Sbjct: 121 RLSQVEKLKTKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA
Sbjct: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK
Sbjct: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300

Query: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338
           LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS
Sbjct: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338

BLAST of Cp4.1LG02g07300 vs. NCBI nr
Match: XP_022982077.1 (calcium uniporter protein 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 657 bits (1695), Expect = 1.14e-237
Identity = 332/338 (98.22%), Postives = 336/338 (99.41%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRRSISKRFLDGFRTNSSPSPINLTSRR+YISPPPDSAGKSVFRRFLQRRAIYHSPSA
Sbjct: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRREYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
           ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII
Sbjct: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQ+EKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF
Sbjct: 121 RLSQVEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA
Sbjct: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK
Sbjct: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300

Query: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338
           LMASQNFDI N+NELKRICYPSYPTQ+E LFSNAHTLS
Sbjct: 301 LMASQNFDIKNFNELKRICYPSYPTQEESLFSNAHTLS 338

BLAST of Cp4.1LG02g07300 vs. NCBI nr
Match: XP_022149376.1 (calcium uniporter protein 4, mitochondrial [Momordica charantia])

HSP 1 Score: 524 bits (1349), Expect = 5.48e-185
Identity = 266/339 (78.47%), Postives = 301/339 (88.79%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRR++SKRFLDG+R   +PS +N + RRDY SPP DSAG+S+FRRFLQRRAIYHSPSA
Sbjct: 1   MALRRTLSKRFLDGYRV--TPSSVNFSFRRDYFSPP-DSAGRSIFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
           ARIPEL+TLP+GD LREKLGG +I+ DRIRLDGLIPP+S +++RS  GCGISIDDARKII
Sbjct: 61  ARIPELMTLPVGDKLREKLGGCSITGDRIRLDGLIPPASLEDQRSGYGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQ+EKLK++LR+I  SSISYSEF RICVE CGN++QG EFAKLLDESG VIV+GNIVF
Sbjct: 121 RLSQVEKLKSRLRDIPTSSISYSEFTRICVEGCGNEEQGTEFAKLLDESGHVIVLGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSME+MIS SIA P+DPR KQLA+ME+QK IIDKKAKAQVQGEL CGLGLL A
Sbjct: 181 LRPDQVAKSMESMISESIATPNDPRFKQLAEMEKQKAIIDKKAKAQVQGELLCGLGLLTA 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLG MRLTFWELSWDVMEPICFFVTSLHFAL Y FFLTTSTEP+FEG FRRRF+AKQKK
Sbjct: 241 QTLGFMRLTFWELSWDVMEPICFFVTSLHFALGYGFFLTTSTEPSFEGFFRRRFRAKQKK 300

Query: 301 LMASQNFDINNYNELKRICYPSYPTQQE-PLFSNAHTLS 338
           LMAS NFDINNYNEL+R CYP++   +E  LFS+ H+LS
Sbjct: 301 LMASYNFDINNYNELRRACYPTHIYNEEGALFSSGHSLS 336

BLAST of Cp4.1LG02g07300 vs. NCBI nr
Match: XP_038905202.1 (calcium uniporter protein 4, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 514 bits (1325), Expect = 2.65e-181
Identity = 269/344 (78.20%), Postives = 297/344 (86.34%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRRSIS RFL      SS SP+ L  RR Y+SPPPDSAG+SVFRRFLQRRAIYHSPSA
Sbjct: 1   MALRRSISHRFL------SSSSPLTLIFRRHYLSPPPDSAGRSVFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSS-FDNERSVSGCGISIDDARKI 120
            RIPE+L++P+GD LREKLG  NI+ DRIRLDGLIPPSS F N  SVSGCGISIDDARKI
Sbjct: 61  PRIPEILSIPVGDRLREKLGASNIAGDRIRLDGLIPPSSNFANGPSVSGCGISIDDARKI 120

Query: 121 IRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGN-KDQGAEFAKLLDESGSVIVIGNI 180
           IRLSQ EK+KAKLR+I K SISYS+F RICVEDCGN ++QGAEFAKLLDESGSVIV+GNI
Sbjct: 121 IRLSQAEKIKAKLRDIPKPSISYSDFTRICVEDCGNNEEQGAEFAKLLDESGSVIVLGNI 180

Query: 181 VFLRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLL 240
           VFLRPDQVAKSMETMISG+IA P+DPRLK+L +ME+QK IIDKKAKAQVQ EL CGLGL+
Sbjct: 181 VFLRPDQVAKSMETMISGAIATPNDPRLKELEEMEKQKAIIDKKAKAQVQAELICGLGLI 240

Query: 241 VAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQ 300
           + QTLG MRLTFWELSWDVMEPICFFVTSLHFAL Y FFL+TSTEPTFEG FRRRF+AKQ
Sbjct: 241 LGQTLGFMRLTFWELSWDVMEPICFFVTSLHFALGYGFFLSTSTEPTFEGFFRRRFRAKQ 300

Query: 301 KKLMASQNFDINNYNELKRICYPSY----PTQQEPLFSNAHTLS 338
           KKLMAS NFDIN YNEL+RICYP++    P ++  LFS  HTLS
Sbjct: 301 KKLMASHNFDINIYNELRRICYPNHRAASPHEEGALFSAPHTLS 338

BLAST of Cp4.1LG02g07300 vs. ExPASy TrEMBL
Match: A0A6J1FPK7 (Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111445822 PE=3 SV=1)

HSP 1 Score: 665 bits (1716), Expect = 3.48e-241
Identity = 336/338 (99.41%), Postives = 337/338 (99.70%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA
Sbjct: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
           ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII
Sbjct: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQ+EKLK KLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF
Sbjct: 121 RLSQVEKLKTKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA
Sbjct: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK
Sbjct: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300

Query: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338
           LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS
Sbjct: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338

BLAST of Cp4.1LG02g07300 vs. ExPASy TrEMBL
Match: A0A6J1IVN2 (Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111481032 PE=3 SV=1)

HSP 1 Score: 657 bits (1695), Expect = 5.54e-238
Identity = 332/338 (98.22%), Postives = 336/338 (99.41%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRRSISKRFLDGFRTNSSPSPINLTSRR+YISPPPDSAGKSVFRRFLQRRAIYHSPSA
Sbjct: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRREYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
           ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII
Sbjct: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQ+EKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF
Sbjct: 121 RLSQVEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA
Sbjct: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK
Sbjct: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300

Query: 301 LMASQNFDINNYNELKRICYPSYPTQQEPLFSNAHTLS 338
           LMASQNFDI N+NELKRICYPSYPTQ+E LFSNAHTLS
Sbjct: 301 LMASQNFDIKNFNELKRICYPSYPTQEESLFSNAHTLS 338

BLAST of Cp4.1LG02g07300 vs. ExPASy TrEMBL
Match: A0A6J1D870 (Calcium uniporter protein OS=Momordica charantia OX=3673 GN=LOC111017780 PE=3 SV=1)

HSP 1 Score: 524 bits (1349), Expect = 2.65e-185
Identity = 266/339 (78.47%), Postives = 301/339 (88.79%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRR++SKRFLDG+R   +PS +N + RRDY SPP DSAG+S+FRRFLQRRAIYHSPSA
Sbjct: 1   MALRRTLSKRFLDGYRV--TPSSVNFSFRRDYFSPP-DSAGRSIFRRFLQRRAIYHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
           ARIPEL+TLP+GD LREKLGG +I+ DRIRLDGLIPP+S +++RS  GCGISIDDARKII
Sbjct: 61  ARIPELMTLPVGDKLREKLGGCSITGDRIRLDGLIPPASLEDQRSGYGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQ+EKLK++LR+I  SSISYSEF RICVE CGN++QG EFAKLLDESG VIV+GNIVF
Sbjct: 121 RLSQVEKLKSRLRDIPTSSISYSEFTRICVEGCGNEEQGTEFAKLLDESGHVIVLGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSME+MIS SIA P+DPR KQLA+ME+QK IIDKKAKAQVQGEL CGLGLL A
Sbjct: 181 LRPDQVAKSMESMISESIATPNDPRFKQLAEMEKQKAIIDKKAKAQVQGELLCGLGLLTA 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLG MRLTFWELSWDVMEPICFFVTSLHFAL Y FFLTTSTEP+FEG FRRRF+AKQKK
Sbjct: 241 QTLGFMRLTFWELSWDVMEPICFFVTSLHFALGYGFFLTTSTEPSFEGFFRRRFRAKQKK 300

Query: 301 LMASQNFDINNYNELKRICYPSYPTQQE-PLFSNAHTLS 338
           LMAS NFDINNYNEL+R CYP++   +E  LFS+ H+LS
Sbjct: 301 LMASYNFDINNYNELRRACYPTHIYNEEGALFSSGHSLS 336

BLAST of Cp4.1LG02g07300 vs. ExPASy TrEMBL
Match: A0A6J1IBJ5 (Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111473692 PE=3 SV=1)

HSP 1 Score: 499 bits (1284), Expect = 1.56e-175
Identity = 249/323 (77.09%), Postives = 280/323 (86.69%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MA RR + KRFLD +RTN     +  + RRDY+SPP DSA K+VFRR L RRAIYHSPS 
Sbjct: 1   MAFRRPLCKRFLDAYRTNPFSPSLCFSLRRDYLSPPMDSAVKAVFRRLLHRRAIYHSPST 60

Query: 61  ARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKII 120
             I ELL+  +G+ LREK+GG NI+ DRIRLDGLIPP+S +NE SVSGCGISIDDARKII
Sbjct: 61  PPISELLSTSVGNKLREKIGGSNITGDRIRLDGLIPPASLENECSVSGCGISIDDARKII 120

Query: 121 RLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIVF 180
           RLSQ+EKLKAKLR+I +SSISYSEF RIC++DCGNKD GAEFAKLLD+SGSVIV+GNIVF
Sbjct: 121 RLSQVEKLKAKLRDIPRSSISYSEFTRICIDDCGNKDDGAEFAKLLDKSGSVIVLGNIVF 180

Query: 181 LRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLVA 240
           LRPDQVAKSMETMIS SIA PDDPR KQL++MERQK  IDKKAKAQVQ ELFCGLGL++ 
Sbjct: 181 LRPDQVAKSMETMISRSIATPDDPRFKQLSEMERQKAAIDKKAKAQVQAELFCGLGLVMG 240

Query: 241 QTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQKK 300
           QTLG MRLTFWELSWDVMEPICFFVTSL+F L Y FFL+TSTEPTFEG+FRRRFKAKQ K
Sbjct: 241 QTLGFMRLTFWELSWDVMEPICFFVTSLYFTLGYGFFLSTSTEPTFEGVFRRRFKAKQTK 300

Query: 301 LMASQNFDINNYNELKRICYPSY 323
           LMAS NFDI+NYNEL+RICYP++
Sbjct: 301 LMASHNFDIDNYNELRRICYPNH 323

BLAST of Cp4.1LG02g07300 vs. ExPASy TrEMBL
Match: A0A0A0LGH7 (Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_3G854770 PE=3 SV=1)

HSP 1 Score: 497 bits (1280), Expect = 1.09e-174
Identity = 263/346 (76.01%), Postives = 288/346 (83.24%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRRS S  F     T+ SPSP     RR Y+SPP DSAGKSVFRRFLQRRAI+HSPSA
Sbjct: 1   MALRRSFSNAFRPSTSTSPSPSPFYSIFRRHYLSPPADSAGKSVFRRFLQRRAIFHSPSA 60

Query: 61  ARIPELLTLPLGDDLREKLG--GRNI-SADRIRLDGLIPPSSFDNERSVSGCGISIDDAR 120
           +RIPEL ++P+GD+LREKLG  G NI S DRIRLDGLI P+S   ERSVSGCGISIDDAR
Sbjct: 61  SRIPELFSMPVGDELREKLGVGGSNITSGDRIRLDGLIHPAS---ERSVSGCGISIDDAR 120

Query: 121 KIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGN-KDQGAEFAKLLDESGSVIVIG 180
           KIIRLSQ+EK+K KLRN+ KS ISYSE+ RIC EDCGN ++QGAEFAKLLDESGSVIV+G
Sbjct: 121 KIIRLSQVEKIKNKLRNVGKSCISYSEYTRICAEDCGNNEEQGAEFAKLLDESGSVIVLG 180

Query: 181 NIVFLRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLG 240
           NIVFLRPDQVA+SME MISGSIA P+DPRLKQL +ME+QK IIDKKAKAQVQ EL CGLG
Sbjct: 181 NIVFLRPDQVARSMERMISGSIATPNDPRLKQLQEMEKQKAIIDKKAKAQVQAELICGLG 240

Query: 241 LLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKA 300
           L++ QTLG MRLTFWELSWDVMEPICFFVTSLHFAL YAFFL TSTEPTFEG FRRRFKA
Sbjct: 241 LILGQTLGFMRLTFWELSWDVMEPICFFVTSLHFALGYAFFLKTSTEPTFEGFFRRRFKA 300

Query: 301 KQKKLMASQNFDINNYNELKRICYPSY----PTQQEPLFSNAHTLS 338
           KQ KLMAS NFDINNYNEL+ ICYP +    P Q  P  S+ H LS
Sbjct: 301 KQTKLMASHNFDINNYNELRTICYPYHTQPLPHQDGPFISSPHALS 343

BLAST of Cp4.1LG02g07300 vs. TAIR 10
Match: AT5G42610.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 271.9 bits (694), Expect = 6.7e-73
Identity = 150/325 (46.15%), Postives = 205/325 (63.08%), Query Frame = 0

Query: 1   MALRRSISKRFLDGFRTNSSPSPINLTSRRDYISPPPDSAGKSVFRRFLQRRAIYHSPSA 60
           MALRR+I+KR +D +R++ +                      S FR+         SP  
Sbjct: 1   MALRRAITKRLIDCYRSDVTQQ-------------------HSFFRQL--------SPYN 60

Query: 61  ARI-PELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERSVSGCGISIDDARKI 120
            +I  + +T P G +L +++ G       IR++ + PP            G+S+ + +K+
Sbjct: 61  MKIHRQYMTSPNGPNLTDRIVG-------IRIESVSPP----RREETPVMGLSVSETKKL 120

Query: 121 IRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKLLDESGSVIVIGNIV 180
           +RL Q+EK+K +LR I KSS+SY EF +IC E CGN +QG++ AK LD SG V+V+G+IV
Sbjct: 121 LRLYQMEKVKERLREIPKSSVSYWEFIQICCESCGNDEQGSQMAKSLDHSGCVVVLGDIV 180

Query: 181 FLRPDQVAKSMETMISGSIARPDDPRLKQLAQMERQKEIIDKKAKAQVQGELFCGLGLLV 240
           FL P Q+AKSME MI  +   P+DPR ++L Q+   K+ ID +A+  VQ EL+CGLG L 
Sbjct: 181 FLHPHQIAKSMEAMIKQTSVLPNDPRKEELVQLATTKKSIDIEARRIVQAELYCGLGFLA 240

Query: 241 AQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEPTFEGIFRRRFKAKQK 300
            QT+G MRLTFWELSWDVMEPICFFVT++HF L Y FFL TSTEP+FEG+FR+RFK KQK
Sbjct: 241 VQTIGFMRLTFWELSWDVMEPICFFVTTIHFILGYIFFLRTSTEPSFEGLFRQRFKTKQK 286

Query: 301 KLMASQNFDINNYNELKRICYPSYP 325
           KLM    FD   YNEL  + + ++P
Sbjct: 301 KLMERHGFDFLRYNELNSL-FTTFP 286

BLAST of Cp4.1LG02g07300 vs. TAIR 10
Match: AT2G23790.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 248.1 bits (632), Expect = 1.0e-65
Identity = 146/334 (43.71%), Postives = 208/334 (62.28%), Query Frame = 0

Query: 1   MALRRSISKRF----------LDGFRTNSSPSPI-----NLTSRRDYISPPPDSAGKSVF 60
           MA+R+ +SK+           L   R +SS   +     N ++    I+P P     S  
Sbjct: 1   MAMRKLLSKKLFNITNVASQSLMNCRISSSSLAVRTRVPNDSTDTTKIAPEPGDLAMS-- 60

Query: 61  RRFLQRRAIYHSPSAARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIPPSSFDNERS 120
           RRF+      H+ +  R  E++ +P+G+ L EKL   + S DRIRLDGL PP     ER 
Sbjct: 61  RRFM------HNSAMIRPAEIMQMPVGESLIEKLREIDGSKDRIRLDGLSPP-----ERE 120

Query: 121 VSGCGISIDDARKIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNKDQGAEFAKL 180
            S   +++ D +K++R +QIE +K+KLR   +S + Y EF  +C E   + D G++ AK+
Sbjct: 121 TS---LTVADTKKLLRAAQIEIVKSKLRETGRSWMPYKEFVSVCGEASSDPDLGSKIAKM 180

Query: 181 LDESGSVIVIGNIVFLRPDQVAKSMETMIS-GSIARPDDPRLKQLAQMERQKEIIDKKAK 240
           LD+S +VIV+G+ V +RPDQV KS+E ++    I  P+DPR  +  ++E +K +ID KA 
Sbjct: 181 LDDSANVIVLGDSVCIRPDQVTKSIEGLLPLPKIHNPNDPRRIEFKELEAEKAVIDVKAH 240

Query: 241 AQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYAFFLTTSTEP 300
             V+ EL+ GLG L+ QT G MRLTFWELSWDVMEPICF+VTS++F   YAFFL TS EP
Sbjct: 241 TLVRKELWAGLGYLILQTAGFMRLTFWELSWDVMEPICFYVTSVYFMAGYAFFLRTSKEP 300

Query: 301 TFEGIFRRRFKAKQKKLMASQNFDINNYNELKRI 319
           +FEG ++ RF+AKQ+KLM    FD+  YNELK++
Sbjct: 301 SFEGFYQSRFEAKQRKLMNEYEFDLERYNELKKL 318

BLAST of Cp4.1LG02g07300 vs. TAIR 10
Match: AT4G36820.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 236.5 bits (602), Expect = 3.1e-62
Identity = 123/286 (43.01%), Postives = 188/286 (65.73%), Query Frame = 0

Query: 37  PDSAGKSVFRRFLQRRAIYHSPSAARIPELLTLPLGDDLREKLGGRNISADRIRLDGLIP 96
           P+    ++ +RFL + ++    + ++      + +G+ L EKL   +++ DRIRLDGL  
Sbjct: 51  PEPGDLTISQRFLNKFSMNGIDTTSK------MSIGESLMEKLKEMDMNKDRIRLDGLSH 110

Query: 97  PSSFDNERSVSGCGISIDDARKIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCGNK 156
           P            G+++ D +K++R ++IE +K KL    K  I YS+F  +C +   + 
Sbjct: 111 PKE-------ETLGLTVQDVKKLLRAAEIEVIKTKLMETGKIWIRYSDFLGVCSDSSLDP 170

Query: 157 DQGAEFAKLLDESGSVIVIGNIVFLRPDQVAKSMETMIS-GSIARPDDPRLKQLAQMERQ 216
            QGA  AK+LD+SG+VIV+GN V LRP Q+ KS+E ++    I  P+DPR K+L ++E  
Sbjct: 171 SQGALIAKMLDDSGNVIVMGNSVCLRPHQLTKSIEGLLPLSQIHNPNDPRRKELNELEAI 230

Query: 217 KEIIDKKAKAQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAYA 276
           K +ID+KA + V+ EL+ GLG L+ QT G MRLTFW+L+WDVMEPICF+V+S++F   Y 
Sbjct: 231 KTVIDQKAHSLVRRELWAGLGYLIIQTAGFMRLTFWDLTWDVMEPICFYVSSVYFMAGYT 290

Query: 277 FFLTTSTEPTFEGIFRRRFKAKQKKLMASQNFDINNYNELKRICYP 322
           FFL TS EP+F+G ++ RF+AKQ+KLM S++FD+  Y+ELK++  P
Sbjct: 291 FFLKTSREPSFQGFYQSRFEAKQRKLMQSEDFDVGRYDELKKLFNP 323

BLAST of Cp4.1LG02g07300 vs. TAIR 10
Match: AT5G66650.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 227.6 bits (579), Expect = 1.5e-59
Identity = 122/288 (42.36%), Postives = 186/288 (64.58%), Query Frame = 0

Query: 36  PPDSAGKSVFRRFLQRRAIYHSPSAARIPELLTLPLGDDLREKLGGRNIS-ADRIRLDGL 95
           PPD+    + RRFL +RA +        PE+  +P G +L EKL    +S  +RIRLD +
Sbjct: 36  PPDAGDSGIRRRFLHKRAFFS-------PEI--VPKGGNLMEKLRELTLSNNNRIRLDEM 95

Query: 96  IPPSSFDNERSVSGCGISIDDARKIIRLSQIEKLKAKLRNIQKSSISYSEFFRICVEDCG 155
           +PP S           ++++D +K++R +++E +K+KLR I K+ + YSEF R+C E   
Sbjct: 96  LPPPSPKKSSPEFFPAVTVEDVKKLMRAAEMELVKSKLREIGKNWVPYSEFVRVCGEYSS 155

Query: 156 NKDQGAEFAKLLDESGSVIVIGNIVFLRPDQVAKSMETMISGSIARPDDPRLKQLAQMER 215
           + +QG   A +LDE+G+VIV+G +V L+P+++  +M  +I       D    ++  Q+E 
Sbjct: 156 DPEQGNRVANMLDEAGNVIVLGKLVCLKPEELTSAMAGLIPTLEPSLDAETRQEFEQLEI 215

Query: 216 QKEIIDKKAKAQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALAY 275
            K  IDK+A   V+ EL+ GLGL++AQT+G  RLTFWELSWDVMEPICF+VTS +F   Y
Sbjct: 216 IKSDIDKRADDLVRKELWAGLGLIMAQTVGFFRLTFWELSWDVMEPICFYVTSTYFMAGY 275

Query: 276 AFFLTTSTEPTFEGIFRRRFKAKQKKLMASQNFDINNYNELKRICYPS 323
           AFFL TS EP+FEG ++ RF+ KQK+L+   +FDI+ + +L+++  P+
Sbjct: 276 AFFLRTSKEPSFEGFYKSRFETKQKRLIKMLDFDIDRFTKLQKMHRPN 314

BLAST of Cp4.1LG02g07300 vs. TAIR 10
Match: AT1G09575.1 (Protein of unknown function (DUF607) )

HSP 1 Score: 129.0 bits (323), Expect = 7.1e-30
Identity = 77/225 (34.22%), Postives = 125/225 (55.56%), Query Frame = 0

Query: 97  PSSFDNERSVSGCGISIDDARKIIRLSQIEKLKAKLRNI-QKSSISYSEFFRIC--VEDC 156
           PS  + E+      I+I +A+K++RL  +E +K KL  +  +  +SYS        +   
Sbjct: 39  PSEDEEEKKKE---ITIAEAKKLMRLVNVEDMKKKLVGVADRDVVSYSTLLEASQGMGIA 98

Query: 157 GNKDQGAEFAKLLDESGSVIVIGNIVFLRPDQVAKSMETMISGSIARPDDPRLKQLAQME 216
            + D+   FA++LD++G V++  + V+L PD+V   +   +       +D   ++  ++ 
Sbjct: 99  RSPDEAHVFARVLDDAGVVLIFRDKVYLHPDKVVDLIRRAMPLDQNPEEDQIKEEFNKLR 158

Query: 217 RQKEIIDKKAKAQVQGELFCGLGLLVAQTLGCMRLTFWELSWDVMEPICFFVTSLHFALA 276
             KE ID  A  QV+  L+CGL   + Q     RLTFWE SWDVMEPI FF T+    + 
Sbjct: 159 IMKEEIDVLAHRQVRKILWCGLATSMVQIGLFFRLTFWEFSWDVMEPITFFATATGIIVG 218

Query: 277 YAFFLTTSTEPTFEGIFRRRFKAKQKKLMASQNFDINNYNELKRI 319
           YA+FL TS +PT++   +R F ++Q+KL+ S  FD   + EL+R+
Sbjct: 219 YAYFLMTSRDPTYQDFMKRLFLSRQRKLLKSHKFDCERFKELERL 260

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJV79.5e-7246.15Calcium uniporter protein 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
O648231.5e-6443.71Calcium uniporter protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q1PE154.4e-6143.01Calcium uniporter protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Q9LVR52.1e-5842.36Calcium uniporter protein 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
F4I1119.9e-2934.22Calcium uniporter protein 6, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At... [more]
Match NameE-valueIdentityDescription
XP_023523255.11.24e-241100.00calcium uniporter protein 4, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022940100.17.20e-24199.41calcium uniporter protein 4, mitochondrial-like [Cucurbita moschata] >KAG6608518... [more]
XP_022982077.11.14e-23798.22calcium uniporter protein 4, mitochondrial [Cucurbita maxima][more]
XP_022149376.15.48e-18578.47calcium uniporter protein 4, mitochondrial [Momordica charantia][more]
XP_038905202.12.65e-18178.20calcium uniporter protein 4, mitochondrial-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1FPK73.48e-24199.41Calcium uniporter protein OS=Cucurbita moschata OX=3662 GN=LOC111445822 PE=3 SV=... [more]
A0A6J1IVN25.54e-23898.22Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111481032 PE=3 SV=1[more]
A0A6J1D8702.65e-18578.47Calcium uniporter protein OS=Momordica charantia OX=3673 GN=LOC111017780 PE=3 SV... [more]
A0A6J1IBJ51.56e-17577.09Calcium uniporter protein OS=Cucurbita maxima OX=3661 GN=LOC111473692 PE=3 SV=1[more]
A0A0A0LGH71.09e-17476.01Calcium uniporter protein OS=Cucumis sativus OX=3659 GN=Csa_3G854770 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G42610.16.7e-7346.15Protein of unknown function (DUF607) [more]
AT2G23790.11.0e-6543.71Protein of unknown function (DUF607) [more]
AT4G36820.13.1e-6243.01Protein of unknown function (DUF607) [more]
AT5G66650.11.5e-5942.36Protein of unknown function (DUF607) [more]
AT1G09575.17.1e-3034.22Protein of unknown function (DUF607) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006769Calcium uniporter protein, C-terminalPFAMPF04678MCUcoord: 156..315
e-value: 2.3E-47
score: 161.4
NoneNo IPR availablePANTHERPTHR13462:SF17CALCIUM UNIPORTER PROTEIN 4, MITOCHONDRIALcoord: 1..322
IPR039055MCU familyPANTHERPTHR13462CALCIUM UNIPORTER PROTEIN, MITOCHONDRIALcoord: 1..322

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g07300.1Cp4.1LG02g07300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
cellular_component GO:0031305 integral component of mitochondrial inner membrane
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005262 calcium channel activity
molecular_function GO:0015292 uniporter activity