Cp4.1LG02g06610 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g06610
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description3-oxo-Delta(4,5)-steroid 5-beta-reductase
LocationCp4.1LG02: 1078945 .. 1080813 (+)
RNA-Seq ExpressionCp4.1LG02g06610
SyntenyCp4.1LG02g06610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGGAAGTAATTACCTTCCATAAACATTCGTTTCTGGGTAGACAGTTGCGTAAGAAAATCCAGTCGTGAGTCCAGCGGTTCGAGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCATCACCTTATAACCCTTTCGACAAACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCACTCCTCCCTAATTCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGGTAACTAACACCCCTAACTCCCTATCTATGCCCCTGTTTCTATTACGTTTCTCTAATCAGCATCTCTGTTCCCAATTTTGCAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCTGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATAATCCGACGCAGCGGCACGATCCGCCGTTCACGGAGGATATGCCTCGATTGGATACCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACCTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGCGAGAGTTGATGAAGGGGAAGAATGAAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTCTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAGATCGTGCCCTGATTTGGCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTCTGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTCGTGATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATGAAGTAATAATCTTTTAGAATGTTGTTAGATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCTCTTCAATGTTCTTCAGCTCATTGAGATTGATGATTACCTCCTTTTG

mRNA sequence

AAAGGAAGTAATTACCTTCCATAAACATTCGTTTCTGGGTAGACAGTTGCGTAAGAAAATCCAGTCGTGAGTCCAGCGGTTCGAGTTGAATGTTTATCCTTTCACAGCTCGCGTCATGGTTGGCGTTTCCTCTGTAATCCACAAGACAACAACGATCTGTAACATCATCATCACCTTATAACCCTTTCGACAAACGATTCAATTCCACTTCAATCTCTGTTTCGCATCTTCCACTCCTCCCTAATTCCCCAAAACCTATCTCCGATTCCCCGACGGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCTGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATAATCCGACGCAGCGGCACGATCCGCCGTTCACGGAGGATATGCCTCGATTGGATACCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACCTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGCGAGAGTTGATGAAGGGGAAGAATGAAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTCTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAGATCGTGCCCTGATTTGGCGAGTAATGTCCATAATTATCAATCTTCTCGTACTGATTTTCATTTCTGGAATCTGTTTGGTTTGGGTTGCTCTGGTTTTACAATAAATGTTGGCTTGTTTATATAGTTTCTTGGTATCTTAGTGACGAGTTCTTCGTGATTCATAGTGTTCCAGCTAGGGAATGATTTCAGTTTGTTTTATGCTTTTCACTCAATGAAGTAATAATCTTTTAGAATGTTGTTAGATTACTGGCTGTCTAATTGTTCGATTCTTGTCACAAACCACAGTTTCCTCTTCAATGTTCTTCAGCTCATTGAGATTGATGATTACCTCCTTTTG

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAATTTGAAGAAGATGAACAACCGAGGAGCTTTGAGAGCGTAGCATTGGTGATCGGCGTTACAGGCATCGTCGGCAACAGCCTTGCCGAGATTCTCCCTCTTTCCGACACCCCCGGCGGTCCGTGGAAGGTGTACGGCGTCGCTCGGAGGCCGAGGCCAAACTGGAACGCCGACCATCCCATCGAATACATTCAATGCGATGTCTCCGATCTGGAAGACGCCGAAGCCAAACTCTCGAAGCTCACTGATGTGACTCATGTGTTCTATGTCACATGGACCAATCTACCCACAGAGATCGAGAACTGTGAGGTCAACGGAAAAATGCTTCGCAACGTGTTGCGATCGGTGATTCCGAACGCGCCAAATCTCCGCCATATCTGCCTTCAAACAGGTACGAAGCACTACATCGGATCGTTCGAGAACATCATCGATAATCCGACGCAGCGGCACGATCCGCCGTTCACGGAGGATATGCCTCGATTGGATACCCCAAATTTCTATTACACTCAAGAAGATTTGTTGTTCGAAGAGATCGAAAAGAGTCAGAAAAAGGATCTGACATGGGCGGTGATCCGACCCAACACGATCTTCGGGTTTTCCCCTTTCAGCTTGATGAACATCGTCGGAACCCTATGCACCTACGCCGCCATATGCAAGCACGAGGGACGTCCGTTGAAATTCCCCGGCACGAAATCAGCTTGGGAGGGTTACTCAGTGGTCTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTAGATCCATACGCAAAGAACGAGGCGTTTAACTGCAGCAATGGCGATGTATTCAAGTGGAAACACTTCTGGAAGGTTTTGGCGGAGCAGTTCAACATCGAGGATTATGGGTTCGAAGAGGGTGAGAGATTGCCGCTGCGAGAGTTGATGAAGGGGAAGAATGAAGTCTGGGAAGAAATTGTGAAGGAGAATCAGTTGCAACCAACAAAATTGGAGGATGTTGCTAATTGGTGGCATACAGATATAGCTCTGAGCGCTTCTGGTAACATAGATAGTATGAACAAGAGCAAGGAGCATGGATTCTTGGGCTTCAGAAACTCCAAGAACTCCTTCATTTCTTGGATCGACAAGGTTAAGGCTTTCAAGATCGTGCCCTGA

Protein sequence

MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNGKMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNFYYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFNIEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDSMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Homology
[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 1.3e-186
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N +D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTN-VDGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 4.6e-179
Identity = 290/393 (73.79%), Postives = 331/393 (84.22%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WN DHPI YI CDV D  D EAKLS LTDVTH+FY TWT   TE ENCE NG
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHY+G+FE   D  ++ HDPP TED+PRLD+ N+
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFE---DLKSKCHDPPLTEDLPRLDSQNY 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE++K  K+ LTW+V RP TIFGFSP+S+MN+VGTLC YAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQK--KEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+GYS  SDADLIAE  IW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +E   +EEG+++ L++LMK K  VW++IV+EN L  TKLEDV NWW +DI L     +D+
Sbjct: 301 VECGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDT 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 626.7 bits (1615), Expect = 1.8e-178
Identity = 290/393 (73.79%), Postives = 329/393 (83.72%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTP GPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP+WN DHPI YI CDV D  D EAKLS LTDVTH+FY TWT   TE ENCE NG
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHY+G+FE   D   +RHDPP TED+PRLD+ NF
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFE---DWKIKRHDPPLTEDLPRLDSQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEE++K  K+ LTW+V RP TIFGFSP+S+MN+VGTLC YAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQK--KESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+GYS  SDADLIAE  IW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +E   +EEG+ + L++LMK K  VW++IV+EN L  TKLEDV NWW +DI L     +D+
Sbjct: 301 VECGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDT 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 2.6e-169
Identity = 283/394 (71.83%), Postives = 313/394 (79.44%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA+KKFEED+ PR  +SVAL+IGVTGIVGNSLAEILP+SDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHP+EYIQCD+SD  D   KLS LTDVTH+F+VTW N PTE E CE+NG
Sbjct: 61  VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
            MLRNVL ++IP A NL HICLQTG KHYIG FE       + H+PPFTEDMPRL+ PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAF--GKIKPHEPPFTEDMPRLNAPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYT ED+L E  EK  K  L W+V RP  IFGFSPFS+MNI+GTLC YAAICKHE  PLK
Sbjct: 181 YYTLEDMLVEASEK--KAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK+AW  YSV SDADLIAE  IW AVDPYAKNEAFNCSNGD+FKWKH WKVLAEQF 
Sbjct: 241 FPGTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEEGER-LPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNID 360
           +E   F+E E+   L E MK K  VWEEIV+EN L  TKLE VA WW  D+ L     +D
Sbjct: 301 VEYAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILGGECLLD 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           SMNKSKEHG+LGFRN+KNS IS IDK+KA KIVP
Sbjct: 361 SMNKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 591.7 bits (1524), Expect = 6.3e-168
Identity = 275/393 (69.97%), Postives = 317/393 (80.66%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARR RP W+ D+PI Y+QCD+SD +D++AKLS LTDVTHVFYVTW N  TE ENCE N 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G FE+      + HDPP+TED+PRL   NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY  ED++ EE+EK  K+ LTW+V RP  IFGFSP+S+MN+VGTLC YAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVEK--KEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           F G K+AW+GYS  SDADLIAE HIW AVDPYAKNEAFN SNGDVFKWKHFWKVLAEQF 
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           +    +EEG  L L++LMKGK  VWEEIV+EN L PTKL+DV  WW  D+ L     +DS
Sbjct: 301 VGCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]
BLAST of Cp4.1LG02g06610 vs. NCBI nr
Match: XP_023523285.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828 bits (2138), Expect = 2.47e-303
Identity = 393/393 (100.00%), Postives = 393/393 (100.00%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. NCBI nr
Match: AJE29749.1 (putative progesterone 5-beta-reductase [Cucurbita pepo])

HSP 1 Score: 822 bits (2123), Expect = 4.79e-301
Identity = 391/393 (99.49%), Postives = 391/393 (99.49%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. NCBI nr
Match: XP_022940360.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037773.1 3-oxo-Delta(4,5)-steroid 5-beta-reductase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 816 bits (2109), Expect = 6.52e-299
Identity = 388/393 (98.73%), Postives = 390/393 (99.24%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. NCBI nr
Match: XP_022981805.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima])

HSP 1 Score: 816 bits (2107), Expect = 1.32e-298
Identity = 386/393 (98.22%), Postives = 390/393 (99.24%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIG+TGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGITGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENC+VNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCDVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNP+ RHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPSHRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. NCBI nr
Match: XP_038899926.1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida])

HSP 1 Score: 739 bits (1907), Expect = 4.03e-268
Identity = 344/393 (87.53%), Postives = 367/393 (93.38%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           +ARRPRP WNADHPIEYIQCD+SD EDAEAKLS+LTDVTH+FYVTWTN PTEIENCE N 
Sbjct: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GS ++I+D   Q HDPPFTED+PRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY+QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSL+NIVGTLC YAAICKHEGR LK
Sbjct: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+KSAWE + V SDADLIAEQHIWTAVDPYAKNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFE+GERL L ELMK K+EVW+EIVKENQLQPTKLE++ NWW TD+  S SGNIDS
Sbjct: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy TrEMBL
Match: A0A0B5CYJ8 (Putative progesterone 5-beta-reductase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 822 bits (2123), Expect = 2.32e-301
Identity = 391/393 (99.49%), Postives = 391/393 (99.49%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           M WWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MRWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADH IEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHLIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy TrEMBL
Match: A0A6J1FQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC111445997 PE=3 SV=1)

HSP 1 Score: 816 bits (2109), Expect = 3.16e-299
Identity = 388/393 (98.73%), Postives = 390/393 (99.24%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIID+PTQRHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDHPTQRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKN VWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNAVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy TrEMBL
Match: A0A6J1J0N7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111480841 PE=3 SV=1)

HSP 1 Score: 816 bits (2107), Expect = 6.38e-299
Identity = 386/393 (98.22%), Postives = 390/393 (99.24%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIG+TGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGITGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRPNWNADHPIEYIQCDVSD EDAEAKLSKLTDVTHVFYVTWTNLPTEIENC+VNG
Sbjct: 61  VARRPRPNWNADHPIEYIQCDVSDPEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCDVNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNP+ RHDPPFTEDMPRLD+PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPSHRHDPPFTEDMPRLDSPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK
Sbjct: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IEDYGFEEGERLPL ELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS
Sbjct: 301 IEDYGFEEGERLPLGELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy TrEMBL
Match: A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)

HSP 1 Score: 728 bits (1878), Expect = 5.13e-264
Identity = 336/393 (85.50%), Postives = 364/393 (92.62%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSD +DAE KLS+L DVTH+FYVTWTN   EI+NCE N 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE++I+  +QRH+PPFTED+PRL+ PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE +   SDADL+AEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEGERL L +LMK K++VW+EIVKENQLQPTKLE++ NWW+ D+  S SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. ExPASy TrEMBL
Match: A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)

HSP 1 Score: 728 bits (1878), Expect = 5.13e-264
Identity = 336/393 (85.50%), Postives = 364/393 (92.62%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDE PRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPIEYIQCDVSD +DAE KLS+L DVTH+FYVTWTN   EI+NCE N 
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GSFE++I+  +QRH+PPFTED+PRL+ PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YY QEDLLFEEIE+SQKKDLTWAVIRPN IFGFSPFSLMNIVGTLC YAAICKHEGRPLK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPGTK AWE +   SDADL+AEQHIWTAVDPY+KNEAFNC+NGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEGERL L +LMK K++VW+EIVKENQLQPTKLE++ NWW+ D+  S SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 393
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of Cp4.1LG02g06610 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 653.7 bits (1685), Expect = 9.6e-188
Identity = 303/393 (77.10%), Postives = 334/393 (84.99%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N +D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTN-VDGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Cp4.1LG02g06610 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 647.1 bits (1668), Expect = 9.0e-186
Identity = 302/393 (76.84%), Postives = 333/393 (84.73%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEQPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA KK +EDE  +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAA-KKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  VARRPRPNWNADHPIEYIQCDVSDLEDAEAKLSKLTDVTHVFYVTWTNLPTEIENCEVNG 120
           VARRPRP WNADHPI+YIQCDVSD ED  +KLS LTDVTHVFYVTWTN  +E ENCE NG
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDTPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHY+G F N +D P  RHDPPFTEDMPRL   NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTN-VDGP--RHDPPFTEDMPRLQIQNF 180

Query: 181 YYTQEDLLFEEIEKSQKKDLTWAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLK 240
           YYTQED+LFEEI+K +   +TW++ RPN IFGFSP+SLMNIVGTLC YAAICKHEG PL 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGTKSAWEGYSVVSDADLIAEQHIWTAVDPYAKNEAFNCSNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEG+   SDADLIAEQ IW AVDPYAKNEAFNC+N D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEEGERLPLRELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGNIDS 360
           IE+YGFEEG+ L L E+MKGK  VWEE+VKENQLQ  KLE+V  WW  D+ L   G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387

[Close]
BLAST of Cp4.1LG02g06610 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 235.3 bits (599), Expect = 8.1e-62
Identity = 142/379 (37.47%), Postives = 210/379 (55.41%), Query Frame = 0 

Query: 26  ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPNWNADHPIEYIQCDVSDL 85
           E+VAL+ GVTG+VG  + + L L   PG  W++YGVAR P  N +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75

Query: 86  EDAEAKLSKLTD-VTHVFYVTWT-NLPTEIENCEVNGK-MLRNVLRSVIPNAPNLRHICL 145
            + + +LS L D V+HVF+VTW+   P + + C V  K ML N L +++PNA  L+H  L
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135

Query: 146 QTGTKHYIGSFENIIDNPTQRHDPPFTEDMPRLDT-PNFYYTQEDLLFEEIEKSQKKDLT 205
           QTG KHY+   E  +          ++E+ PR  +  NFYY  EDLL E+I +S    + 
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRS---SVV 195

Query: 206 WAVIRPNTIFGFSPFSLMNIVGTLCTYAAICKHEGRPLKFPGTKSAW-EGYSVVSDADLI 265
           W+V RP  + G S  +L N +G+LC Y A+CK+   P  F GT+  W E Y   SD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255

Query: 266 AEQHIWTAVDPYA--KNEAFNCSNGDVFKWKHFWKVLAEQFNI---EDYGFEEGERLPLR 325
           AEQHI+ A       K EAFN  NG  F WK  W  + ++  +   E   F+EG     R
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFG-R 315

Query: 326 ELMKGKNEVWEEIVKENQLQPTKLEDVANWWHTDIALSASGN-IDSMNKSKEHGFLGFRN 385
           E+++ K+ VW+EIV + +L  T++ED+ANW+  D         +    K    GF     
Sbjct: 316 EMVERKH-VWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFKRKYR 375

Query: 386 SKNSFISWIDKVKAFKIVP 394
           + +S + WID ++  K++P
Sbjct: 376 TLDSVLYWIDVMRDEKLIP 385

The following BLAST results are available for this feature:
BLAST of Cp4.1LG02g06610 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9STX21.3e-18677.103-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
A0A221J5W84.6e-17973.79(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ... [more]
A0A221J5X31.8e-17873.79(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1... [more]
A0A1C9CX662.6e-16971.83(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... [more]
Q6PQJ96.3e-16869.973-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1[more]
BLAST of Cp4.1LG02g06610 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023523285.12.47e-303100.003-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita pepo subsp. pepo][more]
AJE29749.14.79e-30199.49putative progesterone 5-beta-reductase [Cucurbita pepo][more]
XP_022940360.16.52e-29998.733-oxo-Delta(4,5)-steroid 5-beta-reductase [Cucurbita moschata] >KAG6608438.1 3-o... [more]
XP_022981805.11.32e-29898.223-oxo-Delta(4,5)-steroid 5-beta-reductase-like [Cucurbita maxima][more]
XP_038899926.14.03e-26887.533-oxo-Delta(4,5)-steroid 5-beta-reductase [Benincasa hispida][more]
BLAST of Cp4.1LG02g06610 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0B5CYJ82.32e-30199.49Putative progesterone 5-beta-reductase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1FQC13.16e-29998.733-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1J0N76.38e-29998.223-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A5A7T3B35.13e-26485.503-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BHA75.13e-26485.503-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983... [more]
BLAST of Cp4.1LG02g06610 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G24220.19.6e-18877.10NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.29.0e-18676.84NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G58750.18.1e-6237.47NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 29..256
e-value: 1.3E-4
score: 21.5
NoneNo IPR availableGENE3D3.40.50.720coord: 25..393
e-value: 8.5E-140
score: 467.8
NoneNo IPR availablePANTHERPTHR32487:SF203-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE-LIKEcoord: 1..393
NoneNo IPR availablePANTHERPTHR324873-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 1..393
NoneNo IPR availableCDDcd089485beta-POR_like_SDR_acoord: 28..332
e-value: 1.5272E-136
score: 390.453
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 28..302

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g06610.1Cp4.1LG02g06610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009611 response to wounding
biological_process GO:0008202 steroid metabolic process
biological_process GO:0010051 xylem and phloem pattern formation
molecular_function GO:0047568 3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function GO:0035671 enone reductase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0003824 catalytic activity