Cp4.1LG02g05190 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g05190
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNAD(P)H-hydrate epimerase
LocationCp4.1LG02: 1919023 .. 1923927 (-)
RNA-Seq ExpressionCp4.1LG02g05190
SyntenyCp4.1LG02g05190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAGAATAAAATAATAGGAAGATTAATAAGAATAAAATTCATTGATCCTATCCAACAGTAAGTGTGATAAAAGAACTTGGCCAATTATTTCAAGAAGCAACGGTGACAACTCGTCTTCTGGCGATGCGAAGCGGGTTGAGCCAGCGCAACTCCAATCAGGAATGCGTCCATTTATGCGCACAGCTCTTTCTATGTCACGCTTGATTTCTGCCCACTCATTCCATCTCAAATCGAACTCGATACCGATAATTTCCCATTCTTCTCGCTCTCTTCGAATCCCTCTCTTCAAGCTTCCACAGGTTAGTTCTTCGTACTGTTTTCTGTGTTTTGCCGCCTTTTTTTCTAATCGGTTGTGTAGTAAATTTTGTTGTTAGGGTGTTTATCGGCCGAATTCGAGCTCTGAGTTTCGTTGGTTTTGCTCGAAGTCTCGGATTGCTGCTGCAACCATGCAGAATCGGGAGTCGATTTCGTATCTGACGCAGAAGGAAGCCGCTGAGGTCGATGAAATTCTCATGGGTCCTCTCGGGTTCAGCGTCGATCAGTTGATGGTTAGTTTTGTAATTGCTTGATTCATTGCTAGAAGTTCTTTTGTGAGTTTCAATTTGTGAGTTCAAAATTAGTCTCATTCATCACTTTACAGTACTGAAAATTAAACTGTCGGTGTGTTTTTTTCTTCCCTTTTTTGTTTGTGTGTGTCCAGGAGCTGGCTGGATTGAGCGTAGCCACTTCAATAGCAGAGGTAAGCTTCCTCTTGGCGTTTATTTTCTGTTAAGATCGTTCACACTCGATGGTTAGTGAAAGTAGATTGTATTCTGGCTACTGTTTCGTTCTTGTTTATGAATGATTGTTATGAATCCTTATTTGGCTTATCTTGTTTGGTTAGTGAAAGTAGATATATGTAAGTTAACGTACAATGTTCATTGTTTGGCTAATCTTCTTTTGATTTGCAATTTTCATTGCTCTATATTTGAACCATTAATGAGCATCTTTCTTACGAGTTATAAGGTGGTTTGGGCCAATCTTTGTGTGAGATCCCACATCGGTTGGGGAGGAGAATGAAACATTTTTATAAGAGTGTGGAAACCTCACCCTAGCATACACGTTTTAGAAACCTTGAGGGGAAACTCGAAAGGGAAAGCCCAAAGAGGACAATATCTGCTAGCGGTAGGCTTGGGCCGTTACAAATGGTATATGAGCCAGACATGAGGGATGTGTCGGCGAGGAGGCTGAACCCCGTAGGGAGTGGACACGAGACGGTGTGCCAGCAAGGACGATGGCCCTGAAGAGGGTGGATTGGGGGTCTCACATCGATTGGAGAAGGGAACGAGTGCAAGAGAGGATGTTGGGCCCCAAAGGGGGTGGATTGTGAGATCTCACGTCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGAAACCCTCTCCCTAGCAGACGCATTTTAAAAACCTTGAGAGGAAGCCCGAAAGGGAAAGTCCAAAGAGGACAATATTTGCTAGCAGTGGGCTTGGACCGTTACACTGTGTCTAGCTCAACCAAGTAGGATGAGGAGGTTAAATAGAATTTAAAAAGACATAGTTTTGTTTAGCAAATAGCTGAGAACTTCTGTTTTCCTCCCAAAAAATCAACAGGTTTACAAAGTCGATGAGTATAAGCGCGTTCTTGTTATCTGTGGCCCTGGAAACAATGGTGGAGATGGTCTCGTGGCTGCTCGTCATCTGTTTCACTTCGGATACAATCCATCAATTTGTTATCCAAAGAGGACTCCTAAGCCTCTTTATGCCGGTTTGGTTACTCAGGTTGTGTTTCTCAACCATAATTGTAATCTTTTCCTTGTTCATCACTAGTTTGGCTGAAGTTCTTATCTAATTTCACGGTTTTGTAGCTGGAATCACTGTCAGTTCCTTTCGTGTCTGTAGAGGACTTGCCTTCGGATTTGTCGAAGGATTTTGACATTATAATCGATGCAATCTTTGGGTTCTCCTTCCACGGTAATATCTTCCTGCTATACGCGCTCAATATATTATTTCAAAGAATTAAACTTTCGACCTTACCATCAATTAGTCATTTCTTCTTTAAGATGATCCTAAATTTGTTTCTAATTTGTGGGTTTTTGGGTCGGTTTGTGCATACCTCGACTACTTTCAGGTGCTCCAAGGCCACCATTCAATGATTTGATCCAAAGGCTTGTCTCTTTAAATGAGTACAAGGAAGCAAAGCAAAGATTTCCTGTTGTTGTTTCTGTAGATATTCCATCCGGATGGCATGTCGAAGAAGGGGATATTAGTGATAGCAGCTTTAAACCCGATATGTTGGTATGCTACCGTTCGAATTTTCTACGACCACCGTAGAGGTTTTTTTAATATTTAATTACACAAATTTGTTTAAAAACCGATTAGATCATTAAACACTTATTTATGCTAGGTTTCTCTGACTGCCCCGAAGCTATGTGCAAAGAGATTCCATGGTCCACATCATTTTTTAGGTGGCAGATTTGTGCCACCGTCTATCGTGAGCAAATTTAACCTTCATCTTCCTCCTTATCCCGGCACGGCAATGTGTGTTCGAATAGGAAAGCCTCCACATGTTGATATAGCTGCACTTAGAGAAAACTATATTTCTCCTGAATTTGTTGAAGATAATGTAGAAGCTGATCCTATTCATCAGGTGAATACTATATTCTTATCGGTGTTTACTTATATTGAAATATATCCTCTATAATAAAGTTACTACATTTATGTGAGATCCCACATTAGTTGGAGAGAGGAACGAAGCATTCCTTACAAGGGTGTGGAAACCTCTCTCTAGTAAACGCGTTTTAAAACCGTGAGGCTGACGGCGATACATAACCGGCCAAGGCAGTCAATATCTGCTAACGGTGGGCTTGGACTGTTAGAACTTCCTTATTTGAAAAAGAGTCCTTTGTTAACTTTATTGTTTCTAGTAGTGAACCAGTAGTTTTCTTGCAGTTCCGCAAATGGTTCGATGAAGCAATTGCTGCTAATTTACGGGAACCTAATGCTATGTCGTTGTCTACTGCTACGAGCGATGGAAAACCGTGAGCTTTGATTCTCTTCTTTTGGCATTCACTTAATTGAAGATCATTTTCTCCCTCGCTCAATTTGATAACCCTTTAATTTCTGGTTCACTTTGCTGCTTCAGATCATCTCGTATGGTTTTGCTAAAAGGATTTGATGAAGATGGTTTTGTCTGGTCAGTGTGCTACACTATTATCATGTTGCAGCAAACAATTTTTTCCCGTGCGCCATAAAATAACTCCGCTCATATTGTTCGTTGTTATCTGCAGGTACACAAATTATGGAAGTCAGAAAGCACACGATTTAGCTGAAAATCCACGTGCTTCGCTTCTTTTCTTCTGGGACGGTCTTAATAGGCAGGTGAAATAACAGCTTGCAGTTTTAGTTTGAGTTGAAAATGTCAGTAGGGAGGCCATTTTTTCTGCCCATAAATTCCTCGAGTAACTCGCCCTTCTGTTTCAGTTCCTTTTGGATTCTAATCCATGGGTTCAACATATAAAAAATTAAGCGGTGAGCTTAGGCTGTTACAAATGGTATTATAGAGCCAGTCACCAAGCGGTATGCTAGTGAGGACGTTGGCTCCCAAAAGGGGGGTGGATTGTGAGATCCCACGTTAATTGGAGAGGGGAACGAAACATTCCTTGTAAGGGCGTGAAAACCTCTCCTTAGCAGACGCGTTTTAAAACGTAGAGGGGAAGCCCGAAAGAGAAAGCCCAAAGAGGACAATATCCGCTAGCAATGGGCTTGAGCTGTTACATGTTCTTACACTCGGTCATTAGCTTTTGGCATTTCCTCCACTTCATAATGCTCATCCTCAATGCTTTCAAGCCAATGCAAATGAATGTGCTTACTATATTAGCTATTGTTTGATTGTTACCTTCTAGGTAAGAGTGGAAGGAACAGTGCAGAAAGTTTCCGAAGAGGAATCGGAGCAGTACTTCCACAGTCGTCCTCGTGGAAGTCAGCTCGGTGCAATCGTTAGCCCACAGGTCTGTATCAGTGCCATTCAATATATTGTAGATAATCAAAATGCTGAGATATTGATAGATTCATGATGTCGCTTTCTCATTCAGAGTTCTGTGGTTCCTGGAAGACATTTTCTCATTGATAAGTTCAAAGAACTAGAGGATAGATACTCGAATGGGTATGTGAATCAATATAAGCTCATATCTGATTCAAAATACAAATGATCTTTGATAATTCATTAAGGAAGATGTTCTTCTGTTTGCAGAAGTTTGATTCCAAAACCAAAATACTGGGGCGGATACAGGCTTAAACCAGAAGCTTTTGAGTTTTGGCAGGGGCAGCCATCTCGGCTGCATGACAGGTTATTTTCACCTCTCTAAGAACAGGTGGCTTTATGTCATTGGCCAAGTAGTTGAGTTGCGGCCTTGCAAGCCTTTCTGATACTTAACGTTCGAGCGTTTGAGCGTTTGAGCCCTTCTCAATCTAACTATAACTCAACGGTTAAAATATATGAACGTTTGAGCTTTAAAAAATAACAAACAAAAGGAAATGCACTTTCTTCTCACTCGATTTTTATGCTTGTTTTCTCGACAGGTTGCGGTATTCCCCTTGCGAGATAGAGGGAAAAAAGGTTTGGAAGGTAGAACGGTTGGCTCCGTGATTGCCTCCTCCATTACGTTAGGCTTGGAATTTCATCGTTCATGTACTTTTTCTTTACTTTTCATGCTTGTTTCCCCTCCCCCTCCCCTTTTGTGTTAACGTGGCTAATAAATGATAATTGAATGATTTACTGAGTAATTACAATATTTACGGTTGCCATTAAATTACGTGACAAGTGGTTCTACTACCGACCTCCATAGTGGTATGCTACTATCTACTTTGAACATTAACTCTTAT

mRNA sequence

AAGAGAATAAAATAATAGGAAGATTAATAAGAATAAAATTCATTGATCCTATCCAACAGTAAGTGTGATAAAAGAACTTGGCCAATTATTTCAAGAAGCAACGGTGACAACTCGTCTTCTGGCGATGCGAAGCGGGTTGAGCCAGCGCAACTCCAATCAGGAATGCGTCCATTTATGCGCACAGCTCTTTCTATGTCACGCTTGATTTCTGCCCACTCATTCCATCTCAAATCGAACTCGATACCGATAATTTCCCATTCTTCTCGCTCTCTTCGAATCCCTCTCTTCAAGCTTCCACAGGGTGTTTATCGGCCGAATTCGAGCTCTGAGTTTCGTTGGTTTTGCTCGAAGTCTCGGATTGCTGCTGCAACCATGCAGAATCGGGAGTCGATTTCGTATCTGACGCAGAAGGAAGCCGCTGAGGTCGATGAAATTCTCATGGGTCCTCTCGGGTTCAGCGTCGATCAGTTGATGGAGCTGGCTGGATTGAGCGTAGCCACTTCAATAGCAGAGGTTTACAAAGTCGATGAGTATAAGCGCGTTCTTGTTATCTGTGGCCCTGGAAACAATGGTGGAGATGGTCTCGTGGCTGCTCGTCATCTGTTTCACTTCGGATACAATCCATCAATTTGTTATCCAAAGAGGACTCCTAAGCCTCTTTATGCCGGTTTGGTTACTCAGCTGGAATCACTGTCAGTTCCTTTCGTGTCTGTAGAGGACTTGCCTTCGGATTTGTCGAAGGATTTTGACATTATAATCGATGCAATCTTTGGGTTCTCCTTCCACGGTGCTCCAAGGCCACCATTCAATGATTTGATCCAAAGGCTTGTCTCTTTAAATGAGTACAAGGAAGCAAAGCAAAGATTTCCTGTTGTTGTTTCTGTAGATATTCCATCCGGATGGCATGTCGAAGAAGGGGATATTAGTGATAGCAGCTTTAAACCCGATATGTTGGTTTCTCTGACTGCCCCGAAGCTATGTGCAAAGAGATTCCATGGTCCACATCATTTTTTAGGTGGCAGATTTGTGCCACCGTCTATCGTGAGCAAATTTAACCTTCATCTTCCTCCTTATCCCGGCACGGCAATGTGTGTTCGAATAGGAAAGCCTCCACATGTTGATATAGCTGCACTTAGAGAAAACTATATTTCTCCTGAATTTGTTGAAGATAATGTAGAAGCTGATCCTATTCATCAGTTCCGCAAATGGTTCGATGAAGCAATTGCTGCTAATTTACGGGAACCTAATGCTATGTCGTTGTCTACTGCTACGAGCGATGGAAAACCATCATCTCGTATGGTTTTGCTAAAAGGATTTGATGAAGATGGTTTTGTCTGGTACACAAATTATGGAAGTCAGAAAGCACACGATTTAGCTGAAAATCCACGTGCTTCGCTTCTTTTCTTCTGGGACGGTCTTAATAGGCAGGTAAGAGTGGAAGGAACAGTGCAGAAAGTTTCCGAAGAGGAATCGGAGCAGTACTTCCACAGTCGTCCTCGTGGAAGTCAGCTCGGTGCAATCGTTAGCCCACAGAGTTCTGTGGTTCCTGGAAGACATTTTCTCATTGATAAGTTCAAAGAACTAGAGGATAGATACTCGAATGGAAGTTTGATTCCAAAACCAAAATACTGGGGCGGATACAGGCTTAAACCAGAAGCTTTTGAGTTTTGGCAGGGGCAGCCATCTCGGCTGCATGACAGGTTGCGGTATTCCCCTTGCGAGATAGAGGGAAAAAAGGTTTGGAAGGTAGAACGGTTGGCTCCGTGATTGCCTCCTCCATTACGTTAGGCTTGGAATTTCATCGTTCATGTACTTTTTCTTTACTTTTCATGCTTGTTTCCCCTCCCCCTCCCCTTTTGTGTTAACGTGGCTAATAAATGATAATTGAATGATTTACTGAGTAATTACAATATTTACGGTTGCCATTAAATTACGTGACAAGTGGTTCTACTACCGACCTCCATAGTGGTATGCTACTATCTACTTTGAACATTAACTCTTAT

Coding sequence (CDS)

ATGCGTCCATTTATGCGCACAGCTCTTTCTATGTCACGCTTGATTTCTGCCCACTCATTCCATCTCAAATCGAACTCGATACCGATAATTTCCCATTCTTCTCGCTCTCTTCGAATCCCTCTCTTCAAGCTTCCACAGGGTGTTTATCGGCCGAATTCGAGCTCTGAGTTTCGTTGGTTTTGCTCGAAGTCTCGGATTGCTGCTGCAACCATGCAGAATCGGGAGTCGATTTCGTATCTGACGCAGAAGGAAGCCGCTGAGGTCGATGAAATTCTCATGGGTCCTCTCGGGTTCAGCGTCGATCAGTTGATGGAGCTGGCTGGATTGAGCGTAGCCACTTCAATAGCAGAGGTTTACAAAGTCGATGAGTATAAGCGCGTTCTTGTTATCTGTGGCCCTGGAAACAATGGTGGAGATGGTCTCGTGGCTGCTCGTCATCTGTTTCACTTCGGATACAATCCATCAATTTGTTATCCAAAGAGGACTCCTAAGCCTCTTTATGCCGGTTTGGTTACTCAGCTGGAATCACTGTCAGTTCCTTTCGTGTCTGTAGAGGACTTGCCTTCGGATTTGTCGAAGGATTTTGACATTATAATCGATGCAATCTTTGGGTTCTCCTTCCACGGTGCTCCAAGGCCACCATTCAATGATTTGATCCAAAGGCTTGTCTCTTTAAATGAGTACAAGGAAGCAAAGCAAAGATTTCCTGTTGTTGTTTCTGTAGATATTCCATCCGGATGGCATGTCGAAGAAGGGGATATTAGTGATAGCAGCTTTAAACCCGATATGTTGGTTTCTCTGACTGCCCCGAAGCTATGTGCAAAGAGATTCCATGGTCCACATCATTTTTTAGGTGGCAGATTTGTGCCACCGTCTATCGTGAGCAAATTTAACCTTCATCTTCCTCCTTATCCCGGCACGGCAATGTGTGTTCGAATAGGAAAGCCTCCACATGTTGATATAGCTGCACTTAGAGAAAACTATATTTCTCCTGAATTTGTTGAAGATAATGTAGAAGCTGATCCTATTCATCAGTTCCGCAAATGGTTCGATGAAGCAATTGCTGCTAATTTACGGGAACCTAATGCTATGTCGTTGTCTACTGCTACGAGCGATGGAAAACCATCATCTCGTATGGTTTTGCTAAAAGGATTTGATGAAGATGGTTTTGTCTGGTACACAAATTATGGAAGTCAGAAAGCACACGATTTAGCTGAAAATCCACGTGCTTCGCTTCTTTTCTTCTGGGACGGTCTTAATAGGCAGGTAAGAGTGGAAGGAACAGTGCAGAAAGTTTCCGAAGAGGAATCGGAGCAGTACTTCCACAGTCGTCCTCGTGGAAGTCAGCTCGGTGCAATCGTTAGCCCACAGAGTTCTGTGGTTCCTGGAAGACATTTTCTCATTGATAAGTTCAAAGAACTAGAGGATAGATACTCGAATGGAAGTTTGATTCCAAAACCAAAATACTGGGGCGGATACAGGCTTAAACCAGAAGCTTTTGAGTTTTGGCAGGGGCAGCCATCTCGGCTGCATGACAGGTTGCGGTATTCCCCTTGCGAGATAGAGGGAAAAAAGGTTTGGAAGGTAGAACGGTTGGCTCCGTGA

Protein sequence

MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWFCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYKVDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP
Homology
BLAST of Cp4.1LG02g05190 vs. ExPASy Swiss-Prot
Match: Q9LTX3 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPOX1 PE=1 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 8.1e-219
Identity = 366/535 (68.41%), Postives = 422/535 (78.88%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPII-SHSSRSLRIPLFKLPQGVYRPNSSSEFRW 60
           MR  +R   +M+      +F L+S  +PI  S    SL        Q    P+  S  R 
Sbjct: 1   MRNVIRRVTTMT-----FTFLLQSPPLPISPSPPQFSLSSSPLSKTQRFITPSQGSRLRT 60

Query: 61  FCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVY 120
            C+K  I          +SYLTQ+EAAE+DE LMGPLGFS+DQLMELAGLSVA SIAEVY
Sbjct: 61  LCTKVIIPNMQDSGSPPLSYLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVY 120

Query: 121 KVDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSV 180
           K +EY RVL ICGPGNNGGDGLVAARHL HFGY P ICYPKRT KPLY GLVTQL+SLSV
Sbjct: 121 KPEEYSRVLAICGPGNNGGDGLVAARHLHHFGYKPFICYPKRTAKPLYTGLVTQLDSLSV 180

Query: 181 PFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVV 240
           PFVSVEDLP DLSKDFD+I+DA+FGFSFHGAPRPPF+DLI+RLVSL  Y++  Q+ PV+V
Sbjct: 181 PFVSVEDLPDDLSKDFDVIVDAMFGFSFHGAPRPPFDDLIRRLVSLQNYEQTLQKHPVIV 240

Query: 241 SVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNL 300
           SVDIPSGWHVEEGD  D   KPDMLVSLTAPKLCAKRF GPHHFLGGRFVPPS+  K+ L
Sbjct: 241 SVDIPSGWHVEEGDHEDGGIKPDMLVSLTAPKLCAKRFRGPHHFLGGRFVPPSVAEKYKL 300

Query: 301 HLPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLR 360
            LP YPGT+MCVRIGKPP VDI+A+R NY+SPE +E+ VE DP  QFRKWFDEA+AA LR
Sbjct: 301 ELPSYPGTSMCVRIGKPPKVDISAMRVNYVSPELLEEQVETDPTVQFRKWFDEAVAAGLR 360

Query: 361 EPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGL 420
           E NAM+LSTA  D KPSSRMVLLKGFDE+GFVW+TNY S+K  DL+ENP A+LLF+W+ L
Sbjct: 361 ETNAMALSTANKDKKPSSRMVLLKGFDENGFVWFTNYESKKGSDLSENPSAALLFYWEIL 420

Query: 421 NRQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYS 480
           NRQVR+EG V+++ E ESE YFHSRPRGSQ+GAIVS QSSVVPGRH L D+++EL  +YS
Sbjct: 421 NRQVRIEGPVERIPESESENYFHSRPRGSQIGAIVSKQSSVVPGRHVLYDEYEELTKQYS 480

Query: 481 NGSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 535
           +GS+IPKPK WGG+RLKP  FEFWQGQPSRLHDRL+YS  ++ G   WK+ RLAP
Sbjct: 481 DGSVIPKPKNWGGFRLKPNLFEFWQGQPSRLHDRLQYSLQDVNGNPAWKIHRLAP 530

BLAST of Cp4.1LG02g05190 vs. ExPASy Swiss-Prot
Match: C3YDS7 (NAD(P)H-hydrate epimerase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_104103 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.5e-66
Identity = 125/240 (52.08%), Postives = 162/240 (67.50%), Query Frame = 0

Query: 77  ISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYKVDEYKR---VLVICGP 136
           ISY++Q+EA ++D+ L     +SVDQLMELAG S A ++A+ Y +   K+   VLV CGP
Sbjct: 44  ISYVSQEEAQQIDQELFNEYAYSVDQLMELAGHSCAVALAKSYPLTSLKKDATVLVCCGP 103

Query: 137 GNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSDLSK 196
           GNNGGDGLV ARHL  FGYNPS+ YPKRT KPLY  L  Q E L +PF+S    P  LS 
Sbjct: 104 GNNGGDGLVCARHLKMFGYNPSVFYPKRTDKPLYKNLTIQCEQLDIPFLSHLPKPQLLSD 163

Query: 197 DFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVEEGD 256
            F  I+DA+FGFSF G  RPPF D+++ L         K+    + S+D+PSGW VE G 
Sbjct: 164 GFSYIVDALFGFSFKGEVRPPFGDVLKTL---------KEVTVPICSIDVPSGWDVEGG- 223

Query: 257 ISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMCVRI 314
            +    +P+ L+SLTAPK CA++F G +H+LGGRFVPP I+ K+ L+LP YPGT  C+R+
Sbjct: 224 -NPDGLQPEFLISLTAPKKCAEKFAGRYHYLGGRFVPPGIIQKYELNLPTYPGTEPCIRL 272

BLAST of Cp4.1LG02g05190 vs. ExPASy Swiss-Prot
Match: Q7WG77 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) OX=257310 GN=pdxH PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.6e-65
Identity = 118/216 (54.63%), Postives = 157/216 (72.69%), Query Frame = 0

Query: 319 VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSR 378
           + ++ LR++Y     VE+   A P  QF +WFDEA+AA + EPNAM+L+T  ++G+PS+R
Sbjct: 1   MSVSDLRQSYEKGVLVEEQAAASPFQQFARWFDEAVAARVPEPNAMTLATVNAEGQPSAR 60

Query: 379 MVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEEESE 438
           +VL+KG+D+ GFV++TNY S+K  DL  NPRASLLFFW  L RQVR+EG ++KVS  ES+
Sbjct: 61  IVLIKGYDDAGFVFFTNYESRKGLDLDANPRASLLFFWQPLERQVRIEGVIEKVSAAESD 120

Query: 439 QYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPE 498
           +YFHSRP GS+LGA  S QS  +  R  L  + +E  DRY  G   P+P +WGGYRLKP 
Sbjct: 121 EYFHSRPLGSRLGAWASRQSQPIT-RDELEAREREFRDRY--GEHPPRPPHWGGYRLKPN 180

Query: 499 AFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 535
            FEFWQG+PSRLHDRLRY P   +GK+ W ++RL+P
Sbjct: 181 RFEFWQGRPSRLHDRLRYEP---DGKQGWTIDRLSP 210

BLAST of Cp4.1LG02g05190 vs. ExPASy Swiss-Prot
Match: Q7W4Q1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) OX=257311 GN=pdxH PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.6e-65
Identity = 118/216 (54.63%), Postives = 157/216 (72.69%), Query Frame = 0

Query: 319 VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSR 378
           + ++ LR++Y     VE+   A P  QF +WFDEA+AA + EPNAM+L+T  ++G+PS+R
Sbjct: 1   MSVSDLRQSYEKGVLVEEQAAASPFQQFARWFDEAVAARVPEPNAMTLATVNAEGQPSAR 60

Query: 379 MVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEEESE 438
           +VL+KG+D+ GFV++TNY S+K  DL  NPRASLLFFW  L RQVR+EG ++KVS  ES+
Sbjct: 61  IVLIKGYDDAGFVFFTNYESRKGLDLDANPRASLLFFWQPLERQVRIEGVIEKVSAAESD 120

Query: 439 QYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPE 498
           +YFHSRP GS+LGA  S QS  +  R  L  + +E  DRY  G   P+P +WGGYRLKP 
Sbjct: 121 EYFHSRPLGSRLGAWASRQSQPIT-RDELEAREREFRDRY--GEHPPRPPHWGGYRLKPN 180

Query: 499 AFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 535
            FEFWQG+PSRLHDRLRY P   +GK+ W ++RL+P
Sbjct: 181 RFEFWQGRPSRLHDRLRYEP---DGKQGWTIDRLSP 210

BLAST of Cp4.1LG02g05190 vs. ExPASy Swiss-Prot
Match: Q7VU72 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) OX=257313 GN=pdxH PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 3.6e-65
Identity = 118/216 (54.63%), Postives = 157/216 (72.69%), Query Frame = 0

Query: 319 VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSR 378
           + ++ LR++Y     VE+   A P  QF +WFDEA+AA + EPNAM+L+T  ++G+PS+R
Sbjct: 1   MSVSDLRQSYEKGVLVEEQAAASPFQQFARWFDEAVAARVPEPNAMTLATVNAEGQPSAR 60

Query: 379 MVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEEESE 438
           +VL+KG+D+ GFV++TNY S+K  DL  NPRASLLFFW  L RQVR+EG ++KVS  ES+
Sbjct: 61  IVLIKGYDDAGFVFFTNYESRKGLDLDANPRASLLFFWQPLERQVRIEGVIEKVSAAESD 120

Query: 439 QYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPE 498
           +YFHSRP GS+LGA  S QS  +  R  L  + +E  DRY  G   P+P +WGGYRLKP 
Sbjct: 121 EYFHSRPLGSRLGAWASRQSQPIT-RDELEAREREFRDRY--GEHPPRPPHWGGYRLKPN 180

Query: 499 AFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 535
            FEFWQG+PSRLHDRLRY P   +GK+ W ++RL+P
Sbjct: 181 RFEFWQGRPSRLHDRLRYEP---DGKQGWTIDRLSP 210

BLAST of Cp4.1LG02g05190 vs. NCBI nr
Match: XP_023524154.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1097 bits (2837), Expect = 0.0
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
           VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534

BLAST of Cp4.1LG02g05190 vs. NCBI nr
Match: KAG6608269.1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1078 bits (2789), Expect = 0.0
Identity = 525/534 (98.31%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MRPFMRTALSMSRLISAHSFHLKSNS PIIS SSRSLRIPLF LPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 60

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
           V+EYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGS+KAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEGTVQKVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 534

BLAST of Cp4.1LG02g05190 vs. NCBI nr
Match: KAG7037612.1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1078 bits (2789), Expect = 0.0
Identity = 525/534 (98.31%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MRPFMRTALSMSRLISAHSFHLKSNS PIIS SSRSLRIPLF LPQGVYRPNSSSEFRWF
Sbjct: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK
Sbjct: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
           V+EYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGS+KAHDLAENPRASLLFFWDGLN
Sbjct: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEGTVQKVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 565

BLAST of Cp4.1LG02g05190 vs. NCBI nr
Match: XP_022980894.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 522/534 (97.75%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MRPFMRTALSMSRL+SAHSFHLKSN+ PIISHSSRSLRIPLFKLPQGV RPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLVSAHSFHLKSNATPIISHSSRSLRIPLFKLPQGVCRPNSSSEFRWF 60

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRIAAATMQNRES+SYLTQKEAAEVDEILMGPLGFSV+QLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESVSYLTQKEAAEVDEILMGPLGFSVEQLMELAGLSVATSIAEVYK 120

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
           V+EYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           FVSVEDLP DLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPLDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGS+KAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 534

BLAST of Cp4.1LG02g05190 vs. NCBI nr
Match: XP_022941315.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 525/534 (98.31%), Postives = 527/534 (98.69%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MRPFMRTALSMSRLISAHSFHLKSNS PIIS SSRSLRIPLF LPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 60

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIA VYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAGVYK 120

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
           V+EYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKGFDE GFVWYTNYGS+KAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEYGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534

BLAST of Cp4.1LG02g05190 vs. ExPASy TrEMBL
Match: A0A6J1J0H2 (NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1)

HSP 1 Score: 1076 bits (2783), Expect = 0.0
Identity = 522/534 (97.75%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MRPFMRTALSMSRL+SAHSFHLKSN+ PIISHSSRSLRIPLFKLPQGV RPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLVSAHSFHLKSNATPIISHSSRSLRIPLFKLPQGVCRPNSSSEFRWF 60

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRIAAATMQNRES+SYLTQKEAAEVDEILMGPLGFSV+QLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESVSYLTQKEAAEVDEILMGPLGFSVEQLMELAGLSVATSIAEVYK 120

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
           V+EYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           FVSVEDLP DLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPLDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGS+KAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 534

BLAST of Cp4.1LG02g05190 vs. ExPASy TrEMBL
Match: A0A6J1FT85 (NAD(P)H-hydrate epimerase OS=Cucurbita moschata OX=3662 GN=LOC111446652 PE=3 SV=1)

HSP 1 Score: 1076 bits (2782), Expect = 0.0
Identity = 525/534 (98.31%), Postives = 527/534 (98.69%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MRPFMRTALSMSRLISAHSFHLKSNS PIIS SSRSLRIPLF LPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 60

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIA VYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAGVYK 120

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
           V+EYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKGFDE GFVWYTNYGS+KAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEYGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534

BLAST of Cp4.1LG02g05190 vs. ExPASy TrEMBL
Match: A0A6J1E2J2 (NAD(P)H-hydrate epimerase OS=Momordica charantia OX=3673 GN=LOC111025403 PE=3 SV=1)

HSP 1 Score: 956 bits (2471), Expect = 0.0
Identity = 463/534 (86.70%), Postives = 492/534 (92.13%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60
           MR  +R   +M+R   AHSF LK NS  IISHSSR L+IP  KL QGV RP SSS FRWF
Sbjct: 36  MRSLLRKTQAMTRSFLAHSFSLKPNSSSIISHSSRPLQIPFSKLSQGVSRPYSSSAFRWF 95

Query: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120
           CSKSRI AA+MQN ESISYLTQ+EAAEVDEILMGPLGFSVDQLMELAGLSVATSIAE YK
Sbjct: 96  CSKSRIEAASMQNWESISYLTQREAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEAYK 155

Query: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180
             E KRVL ICGPGNNGGDGLVAARHLFHFGY PSICYPK+TPKPLYAGLVTQLESLSVP
Sbjct: 156 ASECKRVLAICGPGNNGGDGLVAARHLFHFGYKPSICYPKQTPKPLYAGLVTQLESLSVP 215

Query: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240
           F+SVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEA QRFPV+VS
Sbjct: 216 FISVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEADQRFPVIVS 275

Query: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300
           VDIPSGWHVEEGDIS+S+FKPDMLVSLTAPKL AK+FHG HHFLGGRFVPPSIVSK+NLH
Sbjct: 276 VDIPSGWHVEEGDISNSNFKPDMLVSLTAPKLGAKKFHGSHHFLGGRFVPPSIVSKYNLH 335

Query: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEF+E+NVE +PIHQFRKWFDEA+ ANLRE
Sbjct: 336 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFLEENVETNPIHQFRKWFDEAVTANLRE 395

Query: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420
           PNAMSLSTATSDGKPSSRMVLLKG DEDGFVWYTNY S+KAHDLAEN RASLLF+W+GLN
Sbjct: 396 PNAMSLSTATSDGKPSSRMVLLKGVDEDGFVWYTNYESRKAHDLAENARASLLFYWEGLN 455

Query: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480
           RQVRVEG+VQKVSEEESEQYFHSRPRGSQLGAI SPQSSVV GRH LI+++KELEDR+SN
Sbjct: 456 RQVRVEGSVQKVSEEESEQYFHSRPRGSQLGAIASPQSSVVAGRHVLIERYKELEDRFSN 515

Query: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GS IPKPK+WGGYRLKPE FEFWQGQ SRLHDRL+YSPCEI+GK+VWKVERLAP
Sbjct: 516 GSFIPKPKHWGGYRLKPELFEFWQGQQSRLHDRLQYSPCEIDGKRVWKVERLAP 569

BLAST of Cp4.1LG02g05190 vs. ExPASy TrEMBL
Match: A0A6J1J0L7 (NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1)

HSP 1 Score: 947 bits (2448), Expect = 0.0
Identity = 456/464 (98.28%), Postives = 461/464 (99.35%), Query Frame = 0

Query: 71  MQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYKVDEYKRVLVI 130
           MQNRES+SYLTQKEAAEVDEILMGPLGFSV+QLMELAGLSVATSIAEVYKV+EYKRVLVI
Sbjct: 1   MQNRESVSYLTQKEAAEVDEILMGPLGFSVEQLMELAGLSVATSIAEVYKVNEYKRVLVI 60

Query: 131 CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSD 190
           CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLP D
Sbjct: 61  CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPLD 120

Query: 191 LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE 250
           LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE
Sbjct: 121 LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE 180

Query: 251 EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC 310
           EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC
Sbjct: 181 EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC 240

Query: 311 VRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT 370
           VRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT
Sbjct: 241 VRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT 300

Query: 371 SDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLNRQVRVEGTVQ 430
           SDGKPSSRMVLLKGFDEDGFVWYTNYGS+KAHDLAENPRASLLFFWDGLNRQVRVEGTVQ
Sbjct: 301 SDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQ 360

Query: 431 KVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW 490
           KVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW
Sbjct: 361 KVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW 420

Query: 491 GGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 421 GGYRLKPETFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 464

BLAST of Cp4.1LG02g05190 vs. ExPASy TrEMBL
Match: A0A6J1FMZ7 (NAD(P)H-hydrate epimerase OS=Cucurbita moschata OX=3662 GN=LOC111446652 PE=3 SV=1)

HSP 1 Score: 947 bits (2447), Expect = 0.0
Identity = 458/464 (98.71%), Postives = 460/464 (99.14%), Query Frame = 0

Query: 71  MQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYKVDEYKRVLVI 130
           MQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIA VYKV+EYKRVLVI
Sbjct: 1   MQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAGVYKVNEYKRVLVI 60

Query: 131 CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSD 190
           CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSD
Sbjct: 61  CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSD 120

Query: 191 LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE 250
           LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE
Sbjct: 121 LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE 180

Query: 251 EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC 310
           EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC
Sbjct: 181 EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC 240

Query: 311 VRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT 370
           VRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT
Sbjct: 241 VRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT 300

Query: 371 SDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLNRQVRVEGTVQ 430
           SDGKPSSRMVLLKGFDE GFVWYTNYGS+KAHDLAENPRASLLFFWDGLNRQVRVEGTVQ
Sbjct: 301 SDGKPSSRMVLLKGFDEYGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQ 360

Query: 431 KVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW 490
           KVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW
Sbjct: 361 KVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW 420

Query: 491 GGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534
           GGYRLKPE FEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP
Sbjct: 421 GGYRLKPETFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 464

BLAST of Cp4.1LG02g05190 vs. TAIR 10
Match: AT5G49970.1 (pyridoxin (pyrodoxamine) 5'-phosphate oxidase )

HSP 1 Score: 761.1 bits (1964), Expect = 5.8e-220
Identity = 366/535 (68.41%), Postives = 422/535 (78.88%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPII-SHSSRSLRIPLFKLPQGVYRPNSSSEFRW 60
           MR  +R   +M+      +F L+S  +PI  S    SL        Q    P+  S  R 
Sbjct: 1   MRNVIRRVTTMT-----FTFLLQSPPLPISPSPPQFSLSSSPLSKTQRFITPSQGSRLRT 60

Query: 61  FCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVY 120
            C+K  I          +SYLTQ+EAAE+DE LMGPLGFS+DQLMELAGLSVA SIAEVY
Sbjct: 61  LCTKVIIPNMQDSGSPPLSYLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVY 120

Query: 121 KVDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSV 180
           K +EY RVL ICGPGNNGGDGLVAARHL HFGY P ICYPKRT KPLY GLVTQL+SLSV
Sbjct: 121 KPEEYSRVLAICGPGNNGGDGLVAARHLHHFGYKPFICYPKRTAKPLYTGLVTQLDSLSV 180

Query: 181 PFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVV 240
           PFVSVEDLP DLSKDFD+I+DA+FGFSFHGAPRPPF+DLI+RLVSL  Y++  Q+ PV+V
Sbjct: 181 PFVSVEDLPDDLSKDFDVIVDAMFGFSFHGAPRPPFDDLIRRLVSLQNYEQTLQKHPVIV 240

Query: 241 SVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNL 300
           SVDIPSGWHVEEGD  D   KPDMLVSLTAPKLCAKRF GPHHFLGGRFVPPS+  K+ L
Sbjct: 241 SVDIPSGWHVEEGDHEDGGIKPDMLVSLTAPKLCAKRFRGPHHFLGGRFVPPSVAEKYKL 300

Query: 301 HLPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLR 360
            LP YPGT+MCVRIGKPP VDI+A+R NY+SPE +E+ VE DP  QFRKWFDEA+AA LR
Sbjct: 301 ELPSYPGTSMCVRIGKPPKVDISAMRVNYVSPELLEEQVETDPTVQFRKWFDEAVAAGLR 360

Query: 361 EPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGL 420
           E NAM+LSTA  D KPSSRMVLLKGFDE+GFVW+TNY S+K  DL+ENP A+LLF+W+ L
Sbjct: 361 ETNAMALSTANKDKKPSSRMVLLKGFDENGFVWFTNYESKKGSDLSENPSAALLFYWEIL 420

Query: 421 NRQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYS 480
           NRQVR+EG V+++ E ESE YFHSRPRGSQ+GAIVS QSSVVPGRH L D+++EL  +YS
Sbjct: 421 NRQVRIEGPVERIPESESENYFHSRPRGSQIGAIVSKQSSVVPGRHVLYDEYEELTKQYS 480

Query: 481 NGSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 535
           +GS+IPKPK WGG+RLKP  FEFWQGQPSRLHDRL+YS  ++ G   WK+ RLAP
Sbjct: 481 DGSVIPKPKNWGGFRLKPNLFEFWQGQPSRLHDRLQYSLQDVNGNPAWKIHRLAP 530

BLAST of Cp4.1LG02g05190 vs. TAIR 10
Match: AT5G49970.2 (pyridoxin (pyrodoxamine) 5'-phosphate oxidase )

HSP 1 Score: 643.7 bits (1659), Expect = 1.3e-184
Identity = 318/482 (65.98%), Postives = 368/482 (76.35%), Query Frame = 0

Query: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPII-SHSSRSLRIPLFKLPQGVYRPNSSSEFRW 60
           MR  +R   +M+      +F L+S  +PI  S    SL        Q    P+  S  R 
Sbjct: 1   MRNVIRRVTTMT-----FTFLLQSPPLPISPSPPQFSLSSSPLSKTQRFITPSQGSRLRT 60

Query: 61  FCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVY 120
            C+K  I          +SYLTQ+EAAE+DE LMGPLGFS+DQLMELAGLSVA SIAEVY
Sbjct: 61  LCTKVIIPNMQDSGSPPLSYLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVY 120

Query: 121 KVDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSV 180
           K +EY RVL ICGPGNNGGDGLVAARHL HFGY P ICYPKRT KPLY GLVTQL+SLSV
Sbjct: 121 KPEEYSRVLAICGPGNNGGDGLVAARHLHHFGYKPFICYPKRTAKPLYTGLVTQLDSLSV 180

Query: 181 PFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVV 240
           PFVSVEDLP DLSKDFD+I+DA+FGFSFHGAPRPPF+DLI+RLVSL  Y++  Q+ PV+V
Sbjct: 181 PFVSVEDLPDDLSKDFDVIVDAMFGFSFHGAPRPPFDDLIRRLVSLQNYEQTLQKHPVIV 240

Query: 241 SVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNL 300
           SVDIPSGWHVEEGD  D   KPDML           RF GPHHFLGGRFVPPS+  K+ L
Sbjct: 241 SVDIPSGWHVEEGDHEDGGIKPDML-----------RFRGPHHFLGGRFVPPSVAEKYKL 300

Query: 301 HLPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLR 360
            LP YPGT+MCVRIGKPP VDI+A+R NY+SPE +E+ VE DP  QFRKWFDEA+AA LR
Sbjct: 301 ELPSYPGTSMCVRIGKPPKVDISAMRVNYVSPELLEEQVETDPTVQFRKWFDEAVAAGLR 360

Query: 361 EPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGL 420
           E NAM+LSTA  D KPSSRMVLLKGFDE+GFVW+TNY S+K  DL+ENP A+LLF+W+ L
Sbjct: 361 ETNAMALSTANKDKKPSSRMVLLKGFDENGFVWFTNYESKKGSDLSENPSAALLFYWEIL 420

Query: 421 NRQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYS 480
           NRQVR+EG V+++ E ESE YFHSRPRGSQ+GAIVS QSSVVPGRH L D+++EL  +YS
Sbjct: 421 NRQVRIEGPVERIPESESENYFHSRPRGSQIGAIVSKQSSVVPGRHVLYDEYEELTKQYS 466

Query: 481 NG 482
           +G
Sbjct: 481 DG 466

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTX38.1e-21968.41Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic OS=Arabidopsis tha... [more]
C3YDS71.5e-6652.08NAD(P)H-hydrate epimerase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_104103... [more]
Q7WG773.6e-6554.63Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella bronchiseptica (strai... [more]
Q7W4Q13.6e-6554.63Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella parapertussis (strain... [more]
Q7VU723.6e-6554.63Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella pertussis (strain Toh... [more]
Match NameE-valueIdentityDescription
XP_023524154.10.0100.00pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X1 [Cucurb... [more]
KAG6608269.10.098.31Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbit... [more]
KAG7037612.10.098.31Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbit... [more]
XP_022980894.10.097.75pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurb... [more]
XP_022941315.10.098.31pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurb... [more]
Match NameE-valueIdentityDescription
A0A6J1J0H20.097.75NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1[more]
A0A6J1FT850.098.31NAD(P)H-hydrate epimerase OS=Cucurbita moschata OX=3662 GN=LOC111446652 PE=3 SV=... [more]
A0A6J1E2J20.086.70NAD(P)H-hydrate epimerase OS=Momordica charantia OX=3673 GN=LOC111025403 PE=3 SV... [more]
A0A6J1J0L70.098.28NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1[more]
A0A6J1FMZ70.098.71NAD(P)H-hydrate epimerase OS=Cucurbita moschata OX=3662 GN=LOC111446652 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT5G49970.15.8e-22068.41pyridoxin (pyrodoxamine) 5'-phosphate oxidase [more]
AT5G49970.21.3e-18465.98pyridoxin (pyrodoxamine) 5'-phosphate oxidase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011576Pyridoxamine 5'-phosphate oxidase, putativePFAMPF01243Putative_PNPOxcoord: 350..436
e-value: 4.2E-30
score: 103.8
IPR004443YjeF N-terminal domainPFAMPF03853YjeF_Ncoord: 101..273
e-value: 3.4E-35
score: 121.4
IPR004443YjeF N-terminal domainTIGRFAMTIGR00197TIGR00197coord: 82..277
e-value: 1.4E-45
score: 153.4
IPR004443YjeF N-terminal domainHAMAPMF_01966NADHX_epimerasecoord: 81..316
score: 20.531984
IPR004443YjeF N-terminal domainPROSITEPS51385YJEF_Ncoord: 85..321
score: 56.391365
IPR019576Pyridoxine 5'-phosphate oxidase, dimerisation, C-terminalPFAMPF10590PNP_phzG_Ccoord: 490..534
e-value: 1.5E-19
score: 69.7
IPR012349FMN-binding split barrelGENE3D2.30.110.10coord: 328..524
e-value: 5.2E-84
score: 282.6
IPR036652YjeF N-terminal domain superfamilyGENE3D3.40.50.10260coord: 76..319
e-value: 3.5E-74
score: 251.1
IPR036652YjeF N-terminal domain superfamilySUPERFAMILY64153YjeF N-terminal domain-likecoord: 78..308
IPR000659Pyridoxamine 5'-phosphate oxidaseTIGRFAMTIGR00558TIGR00558coord: 317..534
e-value: 4.8E-87
score: 289.0
IPR000659Pyridoxamine 5'-phosphate oxidaseHAMAPMF_01629PdxHcoord: 327..534
score: 39.059021
IPR032976YjeF N-terminal domain-containing protein NAXE-likePANTHERPTHR13232NAD(P)H-HYDRATE EPIMERASEcoord: 71..518
NoneNo IPR availablePANTHERPTHR13232:SF13NAD(P)H-HYDRATE EPIMERASEcoord: 71..518
NoneNo IPR availableSUPERFAMILY50475FMN-binding split barrelcoord: 335..534
IPR019740Pyridoxamine 5'-phosphate oxidase, conserved sitePROSITEPS01064PYRIDOX_OXIDASEcoord: 500..513

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g05190.1Cp4.1LG02g05190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0008615 pyridoxine biosynthetic process
molecular_function GO:0010181 FMN binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0052856 NADHX epimerase activity
molecular_function GO:0052857 NADPHX epimerase activity
molecular_function GO:0004733 pyridoxamine-phosphate oxidase activity
molecular_function GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors