Cp4.1LG02g04620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g04620
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	rust resistance kinase Lr10-like
Location	Cp4.1LG02: 2273829 .. 2276447 (-)
RNA-Seq Expression	Cp4.1LG02g04620
Synteny	Cp4.1LG02g04620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAGATTTAGAGTTAGCTTCCGGTTCGGTGGGTATTTATTGAGTCCAACTGTGTAAACGAGTCTAGTTGCAAGTTCAGGTGGCAAAGGTCGGGCCGACGGCAGCCATGGCGGGTCCTCCCAGTCTCAGTGGCCCCATTAAGATGAAACTCGCCATCGGAAGAAGCTCCGGAAGAGGTCCAGCAGGTCTACGCCACAAACTGAGTAGATTGACTCAATCGGTCAGCTTTGAAATTGAAGTTTCAATCGTCGGTAGATATATTAGGTCTTCCTTTGTCCATTAATTACAAAGATTATTCATGTTGTCCGTCCCTGTCTTGGTTTGAAATCATTGAATGAGATGTGGAAATATTGGCGAGAAATCCGAGCTTTAAATTTCTGTAGGATTTGACTCTGTGAACAGCGACTGTGACGGGATAAGCCATGGCCTTGGGAATGGATCTTCTTCGAAATGTCGTTCCCCTTTTATTACTTTATCTAAGAAAAATACTCTCGAATTGTAGGAGATGGTAAGGCGAAAGAAGAAAAACATAGGAGGAAGAAGCTGCTGTACATGAACAAGATTTAACATTCATCTGATTTTACTACCAAGTAAGTGTAACTCTCTTGATAGCCAACCGAATTTCTTTTGAGCAATATGACTGGATTTGTTTGCTTTCACTGTAATGGCTTTGTTTTTCTTCACAAAATTTGATCAAATTAGTTGTTATTTGCGGCATAACGTTCAGTTGATCAACAATTCAAATTGATAAGTAAGAAAATCAGTGAGGAGTTGTGATTGGAATGGAATACTGAAGAATCAGTGGGAGATATTTAATCCTTTAGTTTCTGGTTCCTTTCAGATAGGTATGTTAAGAGCCAACAAACTTTAGAACAATTAGTGGGAGAGATCTGATTGTTGATTGCACGTAGAAAAAGAGATTAATGTCTGTTCAGACAAGAGAGATGTTCAAGCATAGGTTGATGTTTTGCATTTCAGCTAGCAGTATTCAGTTTGCAGATACCAGATATTTTCCTCTGTATTTATTCGATCTGATTGTTGATTGCGCGTTTGGTAATTTGAAACAAATATGAACACGAAGAACTGCAGATGTCCAATAGTTTGTTGTCCTAGATTAATTTTGTTGTCAAATGTTTTTATGTTGTAAGGTAACGATCAACTTGTCTCCTCATAATGTCGTCGGTCGTTTACTCACCAGATACGAATAGCAGCAGCAATTTTATCACAACTGCTATCTGTGAGTACCTCCTTATCACTGTTTTTTGTGAAAATATACGAAACATTATACTTGACACGAGTTAGACTTCTATTCATCACCACCTTAAACAAAAACATTGGCTTGCAGCTGTAGTAGTAACCTTGGTGATTTTTATTGTCATTGGTGCAATCTCTTGCTGCTTTGCTAGGAAAGCTAAGCAATGTATTGTCACAGAGTTGCGAAATGCAGTTGCTTCATCTGCATCAAATGTGGCACATTCATCTGCCCCAAATGCAGTTCCGGTTTGGGAAATCGATCAACCGACAATGGAAAAGTTCATCAAAGAAATGGCGGAACAGAGGCCTATAAGGTTCACTGCTCATCAACTATACTATTTCACAGGCAATTATTCGACACCACTCGGTTCGGGAGGATTCGGTACGGTCTATAAAGGAGAGTTCCCAAATGGAGTGAAGATTGCGGCGAAGGTTTTGAAGAGGAACTCGGACAAGCAGGCGGAGGAGCAATTTATGAATGAGGTAGGAACGATCGGGAAAACTTACCATATAAACTTGGTTAGGCTTAATGGGTTTTGTTATGACCATTATATGTGTGCACTGGTATTTGAGTACATGGAAAATCGATCTCTGGATAAGTATTTGTTTGGCAAAAATGAAGAACTTGATTGGGGGAAGTTACGTGATGTAGCCATTGGGACTGCTAAAGGTCTTGCTTACTTGCATGAGGAATGCCAACAGAAAATCATCCATTACGACATAAAGCCTGCAAACATTCTGTTGGATGAAAACTTCACTCCCAAAGTTGGTGATTTTGGCCTTGCCAAGCTTTGCAACCGGGACAGCACACATATGTCGCTCACTGGCTATCGTGGCACGCCTGGATATTCAGCACCGGAGTTCTTACTAAAGAACTATCCAATAACCCACAAGTGCGACGTGTATAGCTTCGGAATGGTATTGTTTGAGATCATTGGAAGAAAAAGGAATGGCGGTGTTACTGATTCTGGGAACCCAGATTGGTTTCCACAACATGTGTGGGAGGCTCAGGAAAAGGGTGAATTAGTTGAATTTGTTAGAAGATGTGGGTATGCTGAGGATGATAAAGAGGGAGTGATTTGGACGTGCGAGGTGGCTCTGTGGTGCATTCAGGACTGCCCCGACGAACGACCACCGATGAGTGCGGTGGTGAAGATGCTTGAAGGAGGTGTAGAAATAATGCCACCATCAAAGCCATTTCTGTACTTGGATCCAACTCCAAAAAGGAACAGTAGAGTATCGAATCTACAACAAACAAGCAGCAGTTCAGATTATTCAACCTCAAATGAAGACAGTGCCTCATATTGGTACAAGAAGACTACTCCAATAATGAAGAAGTACGAAATACAGATAGCAAGTTCTTAG

mRNA sequence

AGAAGATTTAGAGTTAGCTTCCGGTTCGGTGGGTATTTATTGAGTCCAACTGTGTAAACGAGTCTAGTTGCAAGTTCAGGTGGCAAAGGTCGGGCCGACGGCAGCCATGGCGGGTCCTCCCAGTCTCAGTGGCCCCATTAAGATGAAACTCGCCATCGGAAGAAGCTCCGGAAGAGGTCCAGCAGGTCTACGCCACAAACTGAGTAGATTGACTCAATCGGTCAGCTTTGAAATTGAAGTTTCAATCGTCGGTAGATATATTAGGAGATGGTAAGGCGAAAGAAGAAAAACATAGGAGGAAGAAGCTGCTGTACATGAACAAGATTTAACATTCATCTGATTTTACTACCAAGTAACGATCAACTTGTCTCCTCATAATGTCGTCGGTCGTTTACTCACCAGATACGAATAGCAGCAGCAATTTTATCACAACTGCTATCTCTGTAGTAGTAACCTTGGTGATTTTTATTGTCATTGGTGCAATCTCTTGCTGCTTTGCTAGGAAAGCTAAGCAATGTATTGTCACAGAGTTGCGAAATGCAGTTGCTTCATCTGCATCAAATGTGGCACATTCATCTGCCCCAAATGCAGTTCCGGTTTGGGAAATCGATCAACCGACAATGGAAAAGTTCATCAAAGAAATGGCGGAACAGAGGCCTATAAGGTTCACTGCTCATCAACTATACTATTTCACAGGCAATTATTCGACACCACTCGGTTCGGGAGGATTCGGTACGGTCTATAAAGGAGAGTTCCCAAATGGAGTGAAGATTGCGGCGAAGGTTTTGAAGAGGAACTCGGACAAGCAGGCGGAGGAGCAATTTATGAATGAGGAAAAGGGTGAATTAGTTGAATTTGTTAGAAGATGTGGGTATGCTGAGGATGATAAAGAGGGAGTGATTTGGACGTGCGAGGTGGCTCTGTGGTGCATTCAGGACTGCCCCGACGAACGACCACCGATGAGTGCGGTGGTGAAGATGCTTGAAGGAGGTGTAGAAATAATGCCACCATCAAAGCCATTTCTGTACTTGGATCCAACTCCAAAAAGGAACAGTAGAGTATCGAATCTACAACAAACAAGCAGCAGTTCAGATTATTCAACCTCAAATGAAGACAGTGCCTCATATTGGTACAAGAAGACTACTCCAATAATGAAGAAGTACGAAATACAGATAGCAAGTTCTTAG

Coding sequence (CDS)

ATGTCGTCGGTCGTTTACTCACCAGATACGAATAGCAGCAGCAATTTTATCACAACTGCTATCTCTGTAGTAGTAACCTTGGTGATTTTTATTGTCATTGGTGCAATCTCTTGCTGCTTTGCTAGGAAAGCTAAGCAATGTATTGTCACAGAGTTGCGAAATGCAGTTGCTTCATCTGCATCAAATGTGGCACATTCATCTGCCCCAAATGCAGTTCCGGTTTGGGAAATCGATCAACCGACAATGGAAAAGTTCATCAAAGAAATGGCGGAACAGAGGCCTATAAGGTTCACTGCTCATCAACTATACTATTTCACAGGCAATTATTCGACACCACTCGGTTCGGGAGGATTCGGTACGGTCTATAAAGGAGAGTTCCCAAATGGAGTGAAGATTGCGGCGAAGGTTTTGAAGAGGAACTCGGACAAGCAGGCGGAGGAGCAATTTATGAATGAGGAAAAGGGTGAATTAGTTGAATTTGTTAGAAGATGTGGGTATGCTGAGGATGATAAAGAGGGAGTGATTTGGACGTGCGAGGTGGCTCTGTGGTGCATTCAGGACTGCCCCGACGAACGACCACCGATGAGTGCGGTGGTGAAGATGCTTGAAGGAGGTGTAGAAATAATGCCACCATCAAAGCCATTTCTGTACTTGGATCCAACTCCAAAAAGGAACAGTAGAGTATCGAATCTACAACAAACAAGCAGCAGTTCAGATTATTCAACCTCAAATGAAGACAGTGCCTCATATTGGTACAAGAAGACTACTCCAATAATGAAGAAGTACGAAATACAGATAGCAAGTTCTTAG

Protein sequence

MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSASNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEEKGELVEFVRRCGYAEDDKEGVIWTCEVALWCIQDCPDERPPMSAVVKMLEGGVEIMPPSKPFLYLDPTPKRNSRVSNLQQTSSSSDYSTSNEDSASYWYKKTTPIMKKYEIQIASS

Homology

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BLAST of Cp4.1LG02g04620 vs. ExPASy Swiss-Prot
Match: P93604 (Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.9e-07
Identity = 27/71 (38.03%), Postives = 43/71 (60.56%), Query Frame = 0


Query: 82  MEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGTVYKGEFPNGVKIAAKVLKRNS 141
           +E F+K     +P R+T  ++      +   +G GGFG+VYKGE PNGV +A K+L+ ++
Sbjct: 314 VEMFLKTYGTSKPTRYTFSEVKKMARRFKEKVGQGGFGSVYKGELPNGVPVAVKMLENST 373

Query: 142 DKQAEEQFMNE 153
            +   E F+NE
Sbjct: 374 GE--GESFINE 382

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy Swiss-Prot
Match: Q3E9X6 (Cysteine-rich receptor-like protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN=CRK21 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.5e-06
Identity = 31/65 (47.69%), Postives = 38/65 (58.46%), Query Frame = 0


Query: 90  AEQRPIRFTAHQLYYFTGNY--STPLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEE 149
           A    +RF    +   T N+  S  LG GGFG VYKG FPNG ++AAK L + SD Q E 
Sbjct: 344 ASSGSLRFDFRAIKAATSNFHKSNKLGHGGFGAVYKGMFPNGTEVAAKRLSKPSD-QGEP 403

Query: 150 QFMNE 153
           +F NE
Sbjct: 404 EFKNE 407

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BLAST of Cp4.1LG02g04620 vs. ExPASy Swiss-Prot
Match: Q9FLV4 (G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabidopsis thaliana OX=3702 GN=At5g24080 PE=2 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 5.5e-06
Identity = 33/86 (38.37%), Postives = 44/86 (51.16%), Query Frame = 0


Query: 94  PIRFTAHQLYYFTGNYSTPLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEE 153
           P+ FT   L   T N+S  LGSGGFGTVYKG       +A K L R +    E +F+ E 
Sbjct: 517 PVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDR-ALSHGEREFITEV 576

Query: 154 K--GEL--VEFVRRCGYAEDDKEGVI 176
              G +  +  VR CGY  +D   ++
Sbjct: 577 NTIGSMHHMNLVRLCGYCSEDSHRLL 601

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy Swiss-Prot
Match: Q9ZT07 (G-type lectin S-receptor-like serine/threonine-protein kinase RKS1 OS=Arabidopsis thaliana OX=3702 GN=RKS1 PE=3 SV=3)

HSP 1 Score: 52.0 bits (123), Expect = 1.2e-05
Identity = 43/111 (38.74%), Postives = 57/111 (51.35%), Query Frame = 0


Query: 47  CIVTELRNAVASSASNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPI-RFTAHQLYYF 106
           C+V E R       SN   SS+ N  PV   D     +F ++ A  R +  F  + +   
Sbjct: 459 CVVRERRK------SNRHRSSSANFAPV-PFDFDESFRFEQDKARNRELPLFDLNTIVAA 518

Query: 107 TGNYST--PLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEEK 155
           T N+S+   LG+GGFG VYKG   N ++IA K L RNS  Q  E+F NE K
Sbjct: 519 TNNFSSQNKLGAGGFGPVYKGVLQNRMEIAVKRLSRNSG-QGMEEFKNEVK 561

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy Swiss-Prot
Match: O64477 (G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 OS=Arabidopsis thaliana OX=3702 GN=At2g19130 PE=2 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.7e-05
Identity = 33/95 (34.74%), Postives = 47/95 (49.47%), Query Frame = 0


Query: 170 DKEGVIWTCEVALWCIQDCPDERPPMSAVVKMLEGGVEIMPPSKP--------------- 229
           D E V   C+VA WCIQD    RP MS VV++LEG +E+ PP  P               
Sbjct: 734 DIEEVTRACKVACWCIQDEESHRPAMSQVVQILEGVLEVNPPPFPRSIQALVVSDEDVVF 793

Query: 230 FLYLDPTPKRNSRVSNLQQTSSSSDYSTSNEDSAS 250
           F     +   NS  ++   +SSSS    +N++S++
Sbjct: 794 FTESSSSSSHNSSQNHKHSSSSSSSKKMTNDNSSA 828

[Close]

BLAST of Cp4.1LG02g04620 vs. NCBI nr
Match: XP_023524202.1 (rust resistance kinase Lr10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524203.1 rust resistance kinase Lr10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524204.1 rust resistance kinase Lr10-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 454 bits (1169), Expect = 4.02e-157
Identity = 269/445 (60.45%), Postives = 269/445 (60.45%), Query Frame = 0


Query: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60
           MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA
Sbjct: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60

Query: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120
           SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT
Sbjct: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120

Query: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNE---------------------------- 180
           VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNE                            
Sbjct: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDHYMCALV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FEYMENRSLDKYLFGKNEELDWGKLRDVAIGTAKGLAYLHEECQQKIIHYDIKPANILLD 240

Query: 241 ------------------------------------------------------------ 269
                                                                       
Sbjct: 241 ENFTPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLLKNYPITHKCDVYSFGMVLF 300

[Close]

BLAST of Cp4.1LG02g04620 vs. NCBI nr
Match: XP_022940209.1 (rust resistance kinase Lr10-like isoform X1 [Cucurbita moschata] >XP_022940210.1 rust resistance kinase Lr10-like isoform X1 [Cucurbita moschata] >XP_022940211.1 rust resistance kinase Lr10-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 426 bits (1096), Expect = 4.90e-146
Identity = 256/445 (57.53%), Postives = 261/445 (58.65%), Query Frame = 0


Query: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60
           MSSVVYSPDTNSSSN  TTA SVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA
Sbjct: 1   MSSVVYSPDTNSSSNSSTTATSVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60

Query: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120
           SNVAHS+APNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLG+GGFGT
Sbjct: 61  SNVAHSTAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGAGGFGT 120

Query: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNE---------------------------- 180
           VYKGEFPNGVKIA KVLKRNS+KQAEEQFMNE                            
Sbjct: 121 VYKGEFPNGVKIAVKVLKRNSEKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDQYMCALV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FEYMENRSLDKYLFAKNEELDWGKLHDVAIGTAKGLAYLHEECQQKIIHYDIKPANILLD 240

Query: 241 ------------------------------------------------------------ 269
                                                                       
Sbjct: 241 ENFTPKVGDFGLAKLCNRDSTHTSLTGYRGTPGYSAPEFLLKNYPITHKCDVYSFGMVLF 300

[Close]

BLAST of Cp4.1LG02g04620 vs. NCBI nr
Match: KAG6608199.1 (PR5-like receptor kinase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 436 bits (1121), Expect = 1.21e-144
Identity = 239/307 (77.85%), Postives = 247/307 (80.46%), Query Frame = 0


Query: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60
           MSSVVYSPDTNSS+N  TTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA
Sbjct: 1   MSSVVYSPDTNSSNNSSTTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60

Query: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120
           SNVAHS+APNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLG+GGFGT
Sbjct: 61  SNVAHSTAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGAGGFGT 120

Query: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEE----KGELVEFVRRCGY---------- 180
           VYKGEFPNGVKIA KVLKRNS+KQAEEQFMNE     K   +  VR  G+          
Sbjct: 121 VYKGEFPNGVKIAVKVLKRNSEKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDQYMCALV 180

Query: 181 --------------------------------AEDDKEGVIWTCEVALWCIQDCPDERPP 240
                                           A+DDKEGVI  CEVALWCIQDCPDERPP
Sbjct: 181 FEYMENRSLDKYLFGKNEELDWRKLQDVAIGTAKDDKEGVIRMCEVALWCIQDCPDERPP 240

Query: 241 MSAVVKMLEGGVEIMPPSKPFLYLDPTPKRNSRVSNLQQTSSSSDYSTSNEDSASYWYKK 261
           MSAVVKMLEGGVEI+PPSKPFLYLDPTPKRNSR SNLQQTSSSSDYSTSNEDSASYWYKK
Sbjct: 241 MSAVVKMLEGGVEIVPPSKPFLYLDPTPKRNSRGSNLQQTSSSSDYSTSNEDSASYWYKK 300

[Close]

BLAST of Cp4.1LG02g04620 vs. NCBI nr
Match: KAG7037558.1 (Rust resistance kinase Lr10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 416 bits (1070), Expect = 1.15e-140
Identity = 224/267 (83.90%), Postives = 229/267 (85.77%), Query Frame = 0


Query: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60
           MSSVVYSPDTNSSSN  TTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA
Sbjct: 1   MSSVVYSPDTNSSSNSSTTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60

Query: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120
           SNVAHS+APNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLG+GGFGT
Sbjct: 61  SNVAHSTAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGAGGFGT 120

Query: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEEKGELVEFVRRCGYAEDDKEGVIWTCEV 180
           VYKGEFPNGVKIA KVLKRNS+K+AEEQFMNE                            
Sbjct: 121 VYKGEFPNGVKIAVKVLKRNSEKRAEEQFMNE---------------------------- 180

Query: 181 ALWCIQDCPDERPPMSAVVKMLEGGVEIMPPSKPFLYLDPTPKRNSRVSNLQQTSSSSDY 240
                 DCPDERPPMSAVVKMLEGGVEI+PPSKPFLYLDPTPKRNSR SNLQQTSSSSDY
Sbjct: 181 ------DCPDERPPMSAVVKMLEGGVEIVPPSKPFLYLDPTPKRNSRGSNLQQTSSSSDY 233

Query: 241 STSNEDSASYWYKKTTPIMKKYEIQIA 267
           STSNEDSASYWYKKTTPIMKKYEIQIA
Sbjct: 241 STSNEDSASYWYKKTTPIMKKYEIQIA 233

[Close]

BLAST of Cp4.1LG02g04620 vs. NCBI nr
Match: XP_022982065.1 (rust resistance kinase Lr10-like isoform X1 [Cucurbita maxima] >XP_022982066.1 rust resistance kinase Lr10-like isoform X1 [Cucurbita maxima] >XP_022982067.1 rust resistance kinase Lr10-like isoform X1 [Cucurbita maxima] >XP_022982068.1 rust resistance kinase Lr10-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 411 bits (1056), Expect = 5.76e-140
Identity = 251/445 (56.40%), Postives = 254/445 (57.08%), Query Frame = 0


Query: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60
           MSSV YSPDTNSSSN  TTA SV VTLVIFIVIG ISCCFARK KQCIVTELRNAVASSA
Sbjct: 1   MSSVDYSPDTNSSSNSSTTATSVGVTLVIFIVIGVISCCFARKVKQCIVTELRNAVASSA 60

Query: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120
           SNVAHSSAP AVPVWEIDQPTMEKFIKEMAEQR IRFTAHQLYYFTGNYSTPLG+GGFGT
Sbjct: 61  SNVAHSSAPYAVPVWEIDQPTMEKFIKEMAEQRAIRFTAHQLYYFTGNYSTPLGAGGFGT 120

Query: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNE---------------------------- 180
           VYKGEFPNGVKIA KVLKRNSDKQAEEQFMNE                            
Sbjct: 121 VYKGEFPNGVKIAVKVLKRNSDKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDQYMCALV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FEYMENRSLDKYLFGKSEELDWRKLQDVAIGTAKGLAYLHEECQQKVIHYDIKPANILLD 240

Query: 241 ------------------------------------------------------------ 269
                                                                       
Sbjct: 241 ENFTPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLLKNYPITHKCDVYSFGMVLF 300

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy TrEMBL
Match: A0A6J1FJD9 (rust resistance kinase Lr10-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445902 PE=3 SV=1)

HSP 1 Score: 426 bits (1096), Expect = 2.37e-146
Identity = 256/445 (57.53%), Postives = 261/445 (58.65%), Query Frame = 0


Query: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60
           MSSVVYSPDTNSSSN  TTA SVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA
Sbjct: 1   MSSVVYSPDTNSSSNSSTTATSVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60

Query: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120
           SNVAHS+APNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLG+GGFGT
Sbjct: 61  SNVAHSTAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGAGGFGT 120

Query: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNE---------------------------- 180
           VYKGEFPNGVKIA KVLKRNS+KQAEEQFMNE                            
Sbjct: 121 VYKGEFPNGVKIAVKVLKRNSEKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDQYMCALV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FEYMENRSLDKYLFAKNEELDWGKLHDVAIGTAKGLAYLHEECQQKIIHYDIKPANILLD 240

Query: 241 ------------------------------------------------------------ 269
                                                                       
Sbjct: 241 ENFTPKVGDFGLAKLCNRDSTHTSLTGYRGTPGYSAPEFLLKNYPITHKCDVYSFGMVLF 300

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy TrEMBL
Match: A0A6J1IVM1 (rust resistance kinase Lr10-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481026 PE=3 SV=1)

HSP 1 Score: 411 bits (1056), Expect = 2.79e-140
Identity = 251/445 (56.40%), Postives = 254/445 (57.08%), Query Frame = 0


Query: 1   MSSVVYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSA 60
           MSSV YSPDTNSSSN  TTA SV VTLVIFIVIG ISCCFARK KQCIVTELRNAVASSA
Sbjct: 1   MSSVDYSPDTNSSSNSSTTATSVGVTLVIFIVIGVISCCFARKVKQCIVTELRNAVASSA 60

Query: 61  SNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGT 120
           SNVAHSSAP AVPVWEIDQPTMEKFIKEMAEQR IRFTAHQLYYFTGNYSTPLG+GGFGT
Sbjct: 61  SNVAHSSAPYAVPVWEIDQPTMEKFIKEMAEQRAIRFTAHQLYYFTGNYSTPLGAGGFGT 120

Query: 121 VYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNE---------------------------- 180
           VYKGEFPNGVKIA KVLKRNSDKQAEEQFMNE                            
Sbjct: 121 VYKGEFPNGVKIAVKVLKRNSDKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDQYMCALV 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 FEYMENRSLDKYLFGKSEELDWRKLQDVAIGTAKGLAYLHEECQQKVIHYDIKPANILLD 240

Query: 241 ------------------------------------------------------------ 269
                                                                       
Sbjct: 241 ENFTPKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLLKNYPITHKCDVYSFGMVLF 300

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy TrEMBL
Match: A0A6J1FNN4 (rust resistance kinase Lr10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445902 PE=3 SV=1)

HSP 1 Score: 288 bits (738), Expect = 3.08e-93
Identity = 179/364 (49.18%), Postives = 183/364 (50.27%), Query Frame = 0


Query: 82  MEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGTVYKGEFPNGVKIAAKVLKRNS 141
           MEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLG+GGFGTVYKGEFPNGVKIA KVLKRNS
Sbjct: 1   MEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGAGGFGTVYKGEFPNGVKIAVKVLKRNS 60

Query: 142 DKQAEEQFMNE------------------------------------------------- 201
           +KQAEEQFMNE                                                 
Sbjct: 61  EKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDQYMCALVFEYMENRSLDKYLFAKNEELD 120

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 121 WGKLHDVAIGTAKGLAYLHEECQQKIIHYDIKPANILLDENFTPKVGDFGLAKLCNRDST 180

Query: 262 ------------------------------------------------------------ 269
                                                                       
Sbjct: 181 HTSLTGYRGTPGYSAPEFLLKNYPITHKCDVYSFGMVLFEIVGRKRNGGVTDSGNPDWFP 240

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy TrEMBL
Match: A0A6J1IYA8 (rust resistance kinase Lr10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481026 PE=3 SV=1)

HSP 1 Score: 283 bits (725), Expect = 2.85e-91
Identity = 178/364 (48.90%), Postives = 181/364 (49.73%), Query Frame = 0


Query: 82  MEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGTVYKGEFPNGVKIAAKVLKRNS 141
           MEKFIKEMAEQR IRFTAHQLYYFTGNYSTPLG+GGFGTVYKGEFPNGVKIA KVLKRNS
Sbjct: 1   MEKFIKEMAEQRAIRFTAHQLYYFTGNYSTPLGAGGFGTVYKGEFPNGVKIAVKVLKRNS 60

Query: 142 DKQAEEQFMNE------------------------------------------------- 201
           DKQAEEQFMNE                                                 
Sbjct: 61  DKQAEEQFMNEVGTIGKTYHINLVRLNGFCYDQYMCALVFEYMENRSLDKYLFGKSEELD 120

Query: 202 ------------------------------------------------------------ 261
                                                                       
Sbjct: 121 WRKLQDVAIGTAKGLAYLHEECQQKVIHYDIKPANILLDENFTPKVGDFGLAKLCNRDST 180

Query: 262 ------------------------------------------------------------ 269
                                                                       
Sbjct: 181 HMSLTGYRGTPGYSAPEFLLKNYPITHKCDVYSFGMVLFEIVGRKRNGGVTDSGNPDWFP 240

[Close]

BLAST of Cp4.1LG02g04620 vs. ExPASy TrEMBL
Match: A0A5A7T688 (Rust resistance kinase Lr10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G006820 PE=3 SV=1)

HSP 1 Score: 279 bits (713), Expect = 1.66e-88
Identity = 185/441 (41.95%), Postives = 212/441 (48.07%), Query Frame = 0


Query: 5   VYSPDTNSSSNFITTAISVVVTLVIFIVIGAISCCFARKAKQCIVTELRNAVASSASNVA 64
           V+SP +++  N   TAI ++++ VIF VIGAISCCFARK +Q IV EL+N VAS+     
Sbjct: 4   VFSPPSDNGENNAGTAIYILLSFVIFAVIGAISCCFARKVRQGIVRELQNVVASAPPTST 63

Query: 65  HSSAPNAVPVWEIDQPTMEKFIKEMAEQRPIRFTAHQLYYFTGNYSTPLGSGGFGTVYKG 124
           HS  PN V +WEIDQPTMEKFI+EMA++RP+RFT  QLY FT NYSTPLGSGGFG+VYKG
Sbjct: 64  HS-VPNVVQIWEIDQPTMEKFIREMAKERPVRFTPQQLYCFTSNYSTPLGSGGFGSVYKG 123

Query: 125 EFPNGVKIAAKVLKRNSDKQAEEQFM---------------------------------- 184
           +FPNGV IA KVLKRNSD+QAEEQFM                                  
Sbjct: 124 QFPNGVNIAVKVLKRNSDRQAEEQFMAEVGTIGRTYHINLVRLYGFCYDQYMGALVFEYM 183

Query: 185 ------------------------------------------------------------ 244
                                                                       
Sbjct: 184 ENGSLDKYLFGKNQDIDWRKLHDVAIGTAKALAYLHEECQQRIIHYDIKPANILLDANFS 243

Query: 245 ------------------------------------------------------------ 269
                                                                       
Sbjct: 244 PKVGDFGLAKLCNRDSTHMSLTGYRGTPGYSAPEFLFYNYPITHKCDVYSFGMVLFEIVG 303

[Close]

BLAST of Cp4.1LG02g04620 vs. TAIR 10
Match: AT1G70250.1 (receptor serine/threonine kinase, putative )

HSP 1 Score: 57.0 bits (136), Expect = 2.7e-08
Identity = 38/99 (38.38%), Postives = 57/99 (57.58%), Query Frame = 0


Query: 153 EKGELVEFVRRCGYAEDDKEGVIWTCEVALWCIQDCPDERPPMSAVVKMLEGGVEIMP-P 212
           EKGE++ F+      E+D++ V     V LWCIQ  P +RPPMS VV+MLEG +E +  P
Sbjct: 685 EKGEIMSFLADQITEEEDEKIVKKMVLVGLWCIQTNPYDRPPMSKVVEMLEGSLEALQIP 744

Query: 213 SKPFLYLDPTPKRNSRVSNLQQTSSSSDYSTSNEDSASY 251
            KP L L       +   ++Q+TSS   +   ++D++ Y
Sbjct: 745 PKPLLCLPAITAPITVDEDIQETSS---FLKPSQDTSYY 780

HSP 2 Score: 46.6 bits (109), Expect = 3.7e-05
Identity = 25/58 (43.10%), Postives = 38/58 (65.52%), Query Frame = 0


Query: 96  RFTAHQLYYFTGNYSTPLGSGGFGTVYKGEFPNGVK-IAAKVLKRNSDKQAEEQFMNE 153
           RF+  Q+   T ++   LG GGFGTVYKG+ P+G + +A K+LK +++    E F+NE
Sbjct: 448 RFSYVQVKKMTKSFENVLGKGGFGTVYKGKLPDGSRDVAVKILKESNED--GEDFINE 503

[Close]

BLAST of Cp4.1LG02g04620 vs. TAIR 10
Match: AT4G23290.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 21 )

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-07
Identity = 31/65 (47.69%), Postives = 38/65 (58.46%), Query Frame = 0


Query: 90  AEQRPIRFTAHQLYYFTGNY--STPLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEE 149
           A    +RF    +   T N+  S  LG GGFG VYKG FPNG ++AAK L + SD Q E 
Sbjct: 254 ASSGSLRFDFRAIKAATSNFHKSNKLGHGGFGAVYKGMFPNGTEVAAKRLSKPSD-QGEP 313

Query: 150 QFMNE 153
           +F NE
Sbjct: 314 EFKNE 317

[Close]

BLAST of Cp4.1LG02g04620 vs. TAIR 10
Match: AT4G23290.2 (cysteine-rich RLK (RECEPTOR-like protein kinase) 21 )

HSP 1 Score: 54.3 bits (129), Expect = 1.8e-07
Identity = 31/65 (47.69%), Postives = 38/65 (58.46%), Query Frame = 0


Query: 90  AEQRPIRFTAHQLYYFTGNY--STPLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEE 149
           A    +RF    +   T N+  S  LG GGFG VYKG FPNG ++AAK L + SD Q E 
Sbjct: 344 ASSGSLRFDFRAIKAATSNFHKSNKLGHGGFGAVYKGMFPNGTEVAAKRLSKPSD-QGEP 403

Query: 150 QFMNE 153
           +F NE
Sbjct: 404 EFKNE 407

[Close]

BLAST of Cp4.1LG02g04620 vs. TAIR 10
Match: AT5G24080.1 (Protein kinase superfamily protein )

HSP 1 Score: 53.1 bits (126), Expect = 3.9e-07
Identity = 33/86 (38.37%), Postives = 44/86 (51.16%), Query Frame = 0


Query: 94  PIRFTAHQLYYFTGNYSTPLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEE 153
           P+ FT   L   T N+S  LGSGGFGTVYKG       +A K L R +    E +F+ E 
Sbjct: 115 PVSFTYRDLQNCTNNFSQLLGSGGFGTVYKGTVAGETLVAVKRLDR-ALSHGEREFITEV 174

Query: 154 K--GEL--VEFVRRCGYAEDDKEGVI 176
              G +  +  VR CGY  +D   ++
Sbjct: 175 NTIGSMHHMNLVRLCGYCSEDSHRLL 199

[Close]

BLAST of Cp4.1LG02g04620 vs. TAIR 10
Match: AT1G11340.1 (S-locus lectin protein kinase family protein )

HSP 1 Score: 52.0 bits (123), Expect = 8.7e-07
Identity = 43/111 (38.74%), Postives = 57/111 (51.35%), Query Frame = 0


Query: 47  CIVTELRNAVASSASNVAHSSAPNAVPVWEIDQPTMEKFIKEMAEQRPI-RFTAHQLYYF 106
           C+V E R       SN   SS+ N  PV   D     +F ++ A  R +  F  + +   
Sbjct: 527 CVVRERRK------SNRHRSSSANFAPV-PFDFDESFRFEQDKARNRELPLFDLNTIVAA 586

Query: 107 TGNYST--PLGSGGFGTVYKGEFPNGVKIAAKVLKRNSDKQAEEQFMNEEK 155
           T N+S+   LG+GGFG VYKG   N ++IA K L RNS  Q  E+F NE K
Sbjct: 587 TNNFSSQNKLGAGGFGPVYKGVLQNRMEIAVKRLSRNSG-QGMEEFKNEVK 629

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g04620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P93604	2.9e-07	38.03	Rust resistance kinase Lr10 OS=Triticum aestivum OX=4565 GN=LRK10 PE=2 SV=1	[more]
Q3E9X6	2.5e-06	47.69	Cysteine-rich receptor-like protein kinase 21 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9FLV4	5.5e-06	38.37	G-type lectin S-receptor-like serine/threonine-protein kinase At5g24080 OS=Arabi...	[more]
Q9ZT07	1.2e-05	38.74	G-type lectin S-receptor-like serine/threonine-protein kinase RKS1 OS=Arabidopsi...	[more]
O64477	2.7e-05	34.74	G-type lectin S-receptor-like serine/threonine-protein kinase At2g19130 OS=Arabi...	[more]

BLAST of Cp4.1LG02g04620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023524202.1	4.02e-157	60.45	rust resistance kinase Lr10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023...	[more]
XP_022940209.1	4.90e-146	57.53	rust resistance kinase Lr10-like isoform X1 [Cucurbita moschata] >XP_022940210.1...	[more]
KAG6608199.1	1.21e-144	77.85	PR5-like receptor kinase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7037558.1	1.15e-140	83.90	Rust resistance kinase Lr10, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_022982065.1	5.76e-140	56.40	rust resistance kinase Lr10-like isoform X1 [Cucurbita maxima] >XP_022982066.1 r...	[more]

BLAST of Cp4.1LG02g04620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FJD9	2.37e-146	57.53	rust resistance kinase Lr10-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IVM1	2.79e-140	56.40	rust resistance kinase Lr10-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1FNN4	3.08e-93	49.18	rust resistance kinase Lr10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IYA8	2.85e-91	48.90	rust resistance kinase Lr10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A5A7T688	1.66e-88	41.95	Rust resistance kinase Lr10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Cp4.1LG02g04620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70250.1	2.7e-08	38.38	receptor serine/threonine kinase, putative	[more]
AT4G23290.1	1.8e-07	47.69	cysteine-rich RLK (RECEPTOR-like protein kinase) 21	[more]
AT4G23290.2	1.8e-07	47.69	cysteine-rich RLK (RECEPTOR-like protein kinase) 21	[more]
AT5G24080.1	3.9e-07	38.37	Protein kinase superfamily protein	[more]
AT1G11340.1	8.7e-07	38.74	S-locus lectin protein kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 79..168 e-value: 1.4E-11 score: 46.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 224..246
None	No IPR available	PANTHER	PTHR27009	RUST RESISTANCE KINASE LR10-RELATED	coord: 163..242 coord: 17..152
None	No IPR available	PANTHER	PTHR27009:SF26	OSJNBB0022F16.11 PROTEIN	coord: 163..242 coord: 17..152
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 113..135
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 84..152

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g04620.1	Cp4.1LG02g04620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016740	transferase activity