Cp4.1LG02g04600 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g04600
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Ribosomal RNA large subunit methyltransferase H
Location	Cp4.1LG02: 2302934 .. 2306421 (-)
RNA-Seq Expression	Cp4.1LG02g04600
Synteny	Cp4.1LG02g04600

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACGGTGGCAACTTGCTAACTGACACGTCATCATCTCCGTCTTAGCCCGAGGGGTCTGGTTTCTGCTCTCGGCTTCTTATCAATGGTTACTGCTCTCTGTGCTAACATTTCCGCTTCTCCTTCAGGTATCATCGTTTTCTCTACAAGAGATGGAAAATAAAAAAAAACCCTAGAATTTGACCTTGGTTTTGTTCGTCTATGTTCTTACCGGCGTTGTGTAATGTTTCTCCGATTTCGAGCTTTCAGTTGGACGTTGCAAGTACACCGGTCAATCTGTGGTACGTTTATTGTTTCTTTCTGTAAATTTAGTTTCGTTGTTTGGTTCTTGTGATTTTTGTCTTCGTTGTGATTCTTTTTCCTTTTTACGGTCTCGGTCGTTCGATTGGCGTTTCGTTGAGTTTTGCAATTCCATTTTGTTAATATCTGATATATTTGGCTGAGATTTCAGTGTGATTGAGGCTCAGGATGACTGAGTTTTAACTGAGATTAGTCTTTTATGGACAATGAACAGAGAGCGGTGCCGATACGCGTTTTAACTGTTGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGACGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGTATGTGATTGTACGGTTGGTTTTCATTTATCAGTTTTCTGATTTACGCTCGAACTTTCAATCTCTGATGAATGATGCTAATCTTAATGAATGCTTTCCATTCCATGTTTCGTTTCTGCATTATAGGGATCCAAGAGCTCAGGTTGATGACGAAGACGTAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTTATCTCTTAACATTCATTTTCGTTGAATCATTACGAGTTTTGTTATTCTGGCTGTGCTCTTTTATTTGATAACTAGTTTAGAACTCTCTTTGTGAGACCTCGCATCGATTAAAGAGGGGAACGAATGCCAGTGAGAACACTAGACTCCAAAGGGGTGGATGGATTGTGAGATCCCACAACGGTTGGAGAGGGGAACGAAACATTCTTTACAAGGGTGTGGAAACCTCTGTCTAGTTTAGAACTAGTTTAGAGCTCTCCCTGTGAGATCCCACATCGATTGAAAAGGGGAACGAATGCCAACAAGGATGCTGGACCCCGAAAGGGGTGGATTGTGAGATCTCACATCGGTTGGAGAGGGGAACAAAGCATTCTTTGTAAGAGTGTGGAAACCTCTCTCAAACATATGCGTTTTAAAACCTTGAGGGGAAGCCTGAAAGGGAAAGCCCAAACAATATCTGCTAGGGGTGGACTTGAACGGTTACAAATCTCTCTATCTCTCTCTCCGTCCCTCAATTACATCATTCTTTTGCCATCTAAAATGTGAAAGAACATTGATTTCTTGTACTTTTTACTTCAAAAGCATGGATCATAGTGCTTCTTTTTCAGAAAAGAAATATAGTTTGCTATGTGCTTTGATTTTATTTTTAGCCCTTCTTCTAAAACTGATCTTTAAAATAGTTTATAATGGTCGTGCTTACTGCTTACAGTGTGTACCATATCCATTCTGCATATGCACCTTCAGGTTGTGATGTTGGATGAGAAAGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGTGAGTACCATTTCCAATTCAAATCAAATGGGTTGCATTGTTATCATCAATTCATCTCTACTGGACTGATTCCTAATAGTTCAAAGTTCAAAGTTAGTTGCCAAGGCCCCATTTGAGAACGGATTGGTTTGGTTTTTTGTTTTCGAAAATTAAGCTTATAAATACTACTTCCACCTATGGAATTCCTTGTTTTGTTATCCACTTCTTACATTTATTTTAGAAAATCGAGCCAAGTTTTGAAAAACTAAGAAAAGTAGTTTTCAAAAACGTGTTTTTGTCTTTAGATCTTGGCTAAGAATTTGTTTTCTCAAGAGAAATGATACGAAAATAAACATAATTTTCAAAAACAGAATAGTTATCAAACAGGACCCGAGGAGTTTGGACTAGAAATTGAGAGTTTTGCTTGAGGCACCTCGAAGTTAAACCAGCCCACATGTTCAACCTCCATGTCAGTCTAGATCTTAATATGTAACAGTCTCCTGCTTTCTAATTTGATGTTAATAAAGTTTTGTATTAATCACCATGGTCCAATTGTTTCTGTAATCAAATTTGTTAGAACTATGGGTGTATCCCAGAATGAAAATGGCATTTCTTATGTTTCTTCTTCTTTGCAGGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCTTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGGTACTTTTTTGTTTCTTCTCTTCTAACCATTAGTGCATTTCGAGTTTCATTTGCAACATCAAATGCTCCTCAAGCAGAGTTTTCCCAAAATGATGTGTTGGGTGGAGTATCCACTTACATTAATGTGATCTTGTACAATAAAGTAACAACTACTACATAGTGAATATATAGGAATCCAAATCCCCGAATTATTCTGATTATGATCTTCTACATCCTAGGTCTTTCTGTTTCTTCATCTGACTTATGTTCTTTCTTTAGCATTCAGATCAAATGATTCTATGAAATTACGTCGATACTTCTACATATTACAAGTTTTGAGAATCCTTAGAATTACCACTGCGATAATAATCAACTTCTACTCAAGAAGTTTGACATATAAAATACTGAGGGACTTTCAGTTTTGCTCAAATCAGCCAAGAATCATGATGCTCCTCTTTCTCTCTCTATCTCCATCTAGAGATTTATTCAAACCTCAGTTCAACCCTGAAAAACACACAAAACTAACAATGCACCATCTTTGCATCTGTGAAGCTTATTGTACAATATAATGCTTATTCTGAATGTATGTATTCTTTTAGTTCATGGACTATTCTGAAGGGTCAGAAGTACCATCATTAGTAGCAGTGTTCGCATGGTATTTTCGTATCTTTTTTTATTTATTGGAAGAGACCGTTTAATTGTAGTTTATTATTATGTCGGGATGAATATTTAGAGGTTTGAATATCATTTTGGTCCATGTATTTTTGTGATGTCTCACATTGGTTGGGGAGGAGAACATGCCACCATTTATAAGGGTGTGGAAACCTTCCCCTAGCAGACGCGTTTTAAAGCCTTGAGAGGAAGCCCGAAAGGGAAAGCCCAAAAAGGACAATATCGGCTAGCGGTGGATCTGGGTCGTTACAATTTTTAGTTCCATTTCATTATTATAGTCTCTTGCCACCAGATGAGGCATCATCTTCCCTGCCCAACCTAGTTGCACCACATGCAGCTCATCAAAATGAAGAGGGTAAGGGGAGTTCTGCCCCGGGGCTAACCCTTCGAGTAGTATTCACTTTTTA

mRNA sequence

CACGGTGGCAACTTGCTAACTGACACGTCATCATCTCCGTCTTAGCCCGAGGGGTCTGGTTTCTGCTCTCGGCTTCTTATCAATGGTTACTGCTCTCTGTGCTAACATTTCCGCTTCTCCTTCAGTTGGACGTTGCAAGTACACCGGTCAATCTGTGAGAGCGGTGCCGATACGCGTTTTAACTGTTGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGACGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGGATCCAAGAGCTCAGGTTGATGACGAAGACGTAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTGTGATGTTGGATGAGAAAGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCTTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGTTCATGGACTATTCTGAAGGGTCAGAAGTACCATCATTAGTAGCAGTGTTCGCATGGTATTTTCGTATCTTTTTTTATTTATTGGAAGAGACCGTTTAATTGTAGTTTATTATTATGTCGGGATGAATATTTAGAGGTTTGAATATCATTTTGGTCCATGTATTTTTGTGATGTCTCACATTGGTTGGGGAGGAGAACATGCCACCATTTATAAGGGTGTGGAAACCTTCCCCTAGCAGACGCGTTTTAAAGCCTTGAGAGGAAGCCCGAAAGGGAAAGCCCAAAAAGGACAATATCGGCTAGCGGTGGATCTGGGTCGTTACAATTTTTAGTTCCATTTCATTATTATAGTCTCTTGCCACCAGATGAGGCATCATCTTCCCTGCCCAACCTAGTTGCACCACATGCAGCTCATCAAAATGAAGAGGGTAAGGGGAGTTCTGCCCCGGGGCTAACCCTTCGAGTAGTATTCACTTTTTA

Coding sequence (CDS)

ATGGTTACTGCTCTCTGTGCTAACATTTCCGCTTCTCCTTCAGTTGGACGTTGCAAGTACACCGGTCAATCTGTGAGAGCGGTGCCGATACGCGTTTTAACTGTTGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGACGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGGATCCAAGAGCTCAGGTTGATGACGAAGACGTAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTGTGATGTTGGATGAGAAAGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCTTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGTTCATGGACTATTCTGAAGGGTCAGAAGTACCATCATTAG

Protein sequence

MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKYHH

Homology

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy Swiss-Prot
Match: Q9LXB4 (Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g10620 PE=3 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.2e-65
Identity = 126/177 (71.19%), Postives = 154/177 (87.01%), Query Frame = 0


Query: 10  SASPS---VGR-CKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQL 69
           SASP+    GR C+Y GQ+VRA+PIRV+TVGKKRS GV+LL+DEY  KL  YC+ ED  +
Sbjct: 17  SASPTPQEKGRTCRYAGQAVRALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLV 76

Query: 70  RSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASR 129
           RSNPRNA+D RAQV+DE+VA++ L+ SDDWVV+LDE+G++I SEQMAEL+ DAGN+GASR
Sbjct: 77  RSNPRNAQDVRAQVEDEEVAMMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASR 136

Query: 130 LAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKYHH 183
           ++FCIGG YGHG Q+R+RANV+I+LSSMVLNHQIAL+VLMEQLY SWTILKGQ YHH
Sbjct: 137 ISFCIGGAYGHGTQVRKRANVTIRLSSMVLNHQIALVVLMEQLYRSWTILKGQNYHH 193

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy Swiss-Prot
Match: A0M0D0 (Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0803) OX=411154 GN=rlmH PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.1e-21
Identity = 59/158 (37.34%), Postives = 91/158 (57.59%), Query Frame = 0


Query: 30  IRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVE-----DVQLRSNPRNARDPRAQVDDEDV 89
           I+++ +GK   + ++ LI  Y D+L +Y   E     D++   N     D   Q   E  
Sbjct: 3   IKLVCIGKTDKKELEALIKIYSDRLQHYIKFEFEIIPDLKKTKN----LDENQQKSKEGE 62

Query: 90  AVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRA 149
            +++ +++ D+VV+LDE GK+ SSE  +E +    NTG  RL F IGGPYG  +++ +RA
Sbjct: 63  LILSGVQNSDFVVLLDENGKQFSSESFSEYIQKRMNTGLKRLIFVIGGPYGFSEEVYKRA 122

Query: 150 NVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKYHH 183
           +  I LS M  +HQ+  L   EQLY ++TILK + YHH
Sbjct: 123 DSKISLSKMTFSHQMVRLFFTEQLYRAFTILKNEPYHH 156

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy Swiss-Prot
Match: Q1DCX5 (Ribosomal RNA large subunit methyltransferase H OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=rlmH PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 5.9e-20
Identity = 57/152 (37.50%), Postives = 88/152 (57.89%), Query Frame = 0


Query: 30  IRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDVAVVNL 89
           +R+L++GK RS   +  + EY  +L +Y   E V++         P      E  A+++ 
Sbjct: 3   VRLLSIGKDRSGLYEPAVQEYAKRLGHYTRFELVEMAEASGKRLKPGDAKAAEAEAILSK 62

Query: 90  LKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRANVSIK 149
            K  DW+V LDE+G  + S +++  V  A  TGA  L F IGG  G   ++R  AN+++ 
Sbjct: 63  RKPQDWLVALDERGSLLDSVELSRYVGKA-QTGAKDLLFIIGGDEGLDARVRDAANLTLS 122

Query: 150 LSSMVLNHQIALLVLMEQLYSSWTILKGQKYH 182
           LS M L H++A +VL+EQLY ++TILKG+ YH
Sbjct: 123 LSKMTLPHRLARVVLIEQLYRAFTILKGEPYH 153

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy Swiss-Prot
Match: B2V1Q9 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain Alaska E43 / Type E3) OX=508767 GN=rlmH PE=3 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.3e-19
Identity = 55/156 (35.26%), Postives = 93/156 (59.62%), Query Frame = 0


Query: 30  IRVLTVGKKRSRGVQLLIDEYIDKLNNYC--NVEDVQLRSNPRNA--RDPRAQVDDEDVA 89
           I +++VGK + + ++  IDEY  +L+ YC  N+ ++Q    P NA  +D     D E   
Sbjct: 3   ITIISVGKLKEKYLKHAIDEYSKRLSRYCKLNIVELQDEQTPDNASEKDELIIKDKEGNK 62

Query: 90  VVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 149
           ++N +K + +V+ LD KGK ISSE++++ + + G  G S L F IGG  G  + + +R+N
Sbjct: 63  ILNSIKDNMYVITLDLKGKMISSEELSKFIDNCGVRGNSNLCFIIGGSLGLSEAVLKRSN 122

Query: 150 VSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKYH 182
            S+  S M   HQ+  ++L+EQ+Y ++ I  G+ YH
Sbjct: 123 YSLCFSKMTFPHQLFRVMLLEQIYRAFRISNGEPYH 158

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy Swiss-Prot
Match: B1KTH1 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain Loch Maree / Type A3) OX=498214 GN=rlmH PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.7e-19
Identity = 54/156 (34.62%), Postives = 93/156 (59.62%), Query Frame = 0


Query: 30  IRVLTVGKKRSRGVQLLIDEYIDKLNNYC--NVEDVQLRSNPRNA--RDPRAQVDDEDVA 89
           I +++VGK + + ++  IDEY  +L+ YC  N+ +V     P NA  ++     + E   
Sbjct: 3   ISIISVGKIKEKFLRAAIDEYSKRLSKYCKLNIIEVTDEKTPDNASLKEENIIREKEGSL 62

Query: 90  VVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 149
           ++  +K +++V+ +D KGK I+SE+ ++L+ +   TG S +AF IGG  G  QQ+  RAN
Sbjct: 63  ILKHIKDNNFVIAIDLKGKSIASEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSRAN 122

Query: 150 VSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKYH 182
             +  S M   HQ+  ++L+EQ+Y ++ IL G+ YH
Sbjct: 123 YKLSFSKMTFPHQLFRVMLLEQVYRAFRILCGEPYH 158

[Close]

BLAST of Cp4.1LG02g04600 vs. NCBI nr
Match: XP_023524400.1 (putative RNA methyltransferase At5g10620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352 bits (903), Expect = 3.77e-122
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV
Sbjct: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN
Sbjct: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY
Sbjct: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. NCBI nr
Match: XP_022940478.1 (putative RNA methyltransferase At5g10620 [Cucurbita moschata] >KAG6608193.1 putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 345 bits (886), Expect = 1.47e-119
Identity = 178/182 (97.80%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           MVTALCA ISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV
Sbjct: 1   MVTALCATISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRNARDPRAQVDDED+AVVNLLKSDDWVVMLDE GKEISSEQMAELVADAGN
Sbjct: 61  EDVQLRSNPRNARDPRAQVDDEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLY SWTILKGQKY
Sbjct: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. NCBI nr
Match: XP_022982492.1 (putative RNA methyltransferase At5g10620 [Cucurbita maxima] >XP_022982493.1 putative RNA methyltransferase At5g10620 [Cucurbita maxima])

HSP 1 Score: 338 bits (868), Expect = 8.17e-117
Identity = 174/182 (95.60%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           MVTALCA ISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV
Sbjct: 1   MVTALCATISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRN RD RAQVDDED+AVVNLLKSDDWVVMLDE GKEISSEQMAELVADAGN
Sbjct: 61  EDVQLRSNPRNTRDARAQVDDEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           TGASRL+FCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIA+LVLMEQLY SWTILKGQKY
Sbjct: 121 TGASRLSFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIAVLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. NCBI nr
Match: XP_008466330.1 (PREDICTED: putative RNA methyltransferase At5g10620 [Cucumis melo] >XP_008466331.1 PREDICTED: putative RNA methyltransferase At5g10620 [Cucumis melo])

HSP 1 Score: 325 bits (832), Expect = 2.52e-111
Identity = 165/182 (90.66%), Postives = 175/182 (96.15%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           M+TAL   +SASPS GRCKYTGQSVRAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+V
Sbjct: 1   MITALSTALSASPSGGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRNARDP+AQ+DDEDVAVVNLLKSDDWVVMLDE GKEISSEQMAELVADAGN
Sbjct: 61  EDVQLRSNPRNARDPKAQIDDEDVAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           TGASRL+FCIGGPYGHGQ+LR+RAN+SIKLSSMVLNHQIALLVLMEQLY SWTILKGQKY
Sbjct: 121 TGASRLSFCIGGPYGHGQKLRERANLSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. NCBI nr
Match: XP_011652513.1 (putative RNA methyltransferase At5g10620 isoform X4 [Cucumis sativus])

HSP 1 Score: 320 bits (820), Expect = 1.70e-109
Identity = 164/182 (90.11%), Postives = 172/182 (94.51%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           M+TAL   +SASPS GRCKYTGQSVRAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+V
Sbjct: 1   MITALSTALSASPSGGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRNARDP+AQVDDEDVAVVNLLK DDWVVMLDE GKE+SSE MAELVADAGN
Sbjct: 61  EDVQLRSNPRNARDPKAQVDDEDVAVVNLLKPDDWVVMLDENGKELSSELMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           T ASRL+FCIGGPYGHGQQLRQRAN+SIKLSSMVLNHQIALLVLMEQLY SWTILKGQKY
Sbjct: 121 TSASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy TrEMBL
Match: A0A6J1FPF2 (putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111446064 PE=3 SV=1)

HSP 1 Score: 345 bits (886), Expect = 7.13e-120
Identity = 178/182 (97.80%), Postives = 179/182 (98.35%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           MVTALCA ISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV
Sbjct: 1   MVTALCATISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRNARDPRAQVDDED+AVVNLLKSDDWVVMLDE GKEISSEQMAELVADAGN
Sbjct: 61  EDVQLRSNPRNARDPRAQVDDEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLY SWTILKGQKY
Sbjct: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy TrEMBL
Match: A0A6J1IWR3 (putative RNA methyltransferase At5g10620 OS=Cucurbita maxima OX=3661 GN=LOC111481296 PE=3 SV=1)

HSP 1 Score: 338 bits (868), Expect = 3.96e-117
Identity = 174/182 (95.60%), Postives = 177/182 (97.25%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           MVTALCA ISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV
Sbjct: 1   MVTALCATISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRN RD RAQVDDED+AVVNLLKSDDWVVMLDE GKEISSEQMAELVADAGN
Sbjct: 61  EDVQLRSNPRNTRDARAQVDDEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           TGASRL+FCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIA+LVLMEQLY SWTILKGQKY
Sbjct: 121 TGASRLSFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIAVLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy TrEMBL
Match: A0A1S3CQZ8 (putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770 PE=3 SV=1)

HSP 1 Score: 325 bits (832), Expect = 1.22e-111
Identity = 165/182 (90.66%), Postives = 175/182 (96.15%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           M+TAL   +SASPS GRCKYTGQSVRAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+V
Sbjct: 1   MITALSTALSASPSGGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRNARDP+AQ+DDEDVAVVNLLKSDDWVVMLDE GKEISSEQMAELVADAGN
Sbjct: 61  EDVQLRSNPRNARDPKAQIDDEDVAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           TGASRL+FCIGGPYGHGQ+LR+RAN+SIKLSSMVLNHQIALLVLMEQLY SWTILKGQKY
Sbjct: 121 TGASRLSFCIGGPYGHGQKLRERANLSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy TrEMBL
Match: A0A0A0LGS0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881670 PE=3 SV=1)

HSP 1 Score: 320 bits (820), Expect = 8.22e-110
Identity = 164/182 (90.11%), Postives = 172/182 (94.51%), Query Frame = 0


Query: 1   MVTALCANISASPSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNV 60
           M+TAL   +SASPS GRCKYTGQSVRAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+V
Sbjct: 1   MITALSTALSASPSGGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHV 60

Query: 61  EDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGN 120
           EDVQLRSNPRNARDP+AQVDDEDVAVVNLLK DDWVVMLDE GKE+SSE MAELVADAGN
Sbjct: 61  EDVQLRSNPRNARDPKAQVDDEDVAVVNLLKPDDWVVMLDENGKELSSELMAELVADAGN 120

Query: 121 TGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180
           T ASRL+FCIGGPYGHGQQLRQRAN+SIKLSSMVLNHQIALLVLMEQLY SWTILKGQKY
Sbjct: 121 TSASRLSFCIGGPYGHGQQLRQRANLSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

Query: 181 HH 182
           HH
Sbjct: 181 HH 182

[Close]

BLAST of Cp4.1LG02g04600 vs. ExPASy TrEMBL
Match: A0A6J1BPR4 (putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 318 bits (816), Expect = 3.85e-109
Identity = 164/186 (88.17%), Postives = 177/186 (95.16%), Query Frame = 0


Query: 1   MVTALCANISAS----PSVGRCKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNN 60
           MV+ALCA++SAS    PS GRCKYT QSVRAVPIRVLTVGKKRSRGVQL++DEYI+KL +
Sbjct: 1   MVSALCASLSASASPSPSGGRCKYTAQSVRAVPIRVLTVGKKRSRGVQLVVDEYIEKLKH 60

Query: 61  YCNVEDVQLRSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVA 120
           YCNVEDVQLRSNPRNARDPRAQVDDED+AVVNLLKSDDWVVMLDE GKE+SSE+MAELVA
Sbjct: 61  YCNVEDVQLRSNPRNARDPRAQVDDEDMAVVNLLKSDDWVVMLDENGKEMSSERMAELVA 120

Query: 121 DAGNTGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILK 180
           DAGNTGASRL+FCIGGPYGHGQQLRQRAN++IKLSSMVLNHQIALLVLMEQLY SWTILK
Sbjct: 121 DAGNTGASRLSFCIGGPYGHGQQLRQRANLTIKLSSMVLNHQIALLVLMEQLYRSWTILK 180

Query: 181 GQKYHH 182
           GQKYHH
Sbjct: 181 GQKYHH 186

[Close]

BLAST of Cp4.1LG02g04600 vs. TAIR 10
Match: AT5G10620.1 (methyltransferases )

HSP 1 Score: 250.8 bits (639), Expect = 8.7e-67
Identity = 126/177 (71.19%), Postives = 154/177 (87.01%), Query Frame = 0


Query: 10  SASPS---VGR-CKYTGQSVRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQL 69
           SASP+    GR C+Y GQ+VRA+PIRV+TVGKKRS GV+LL+DEY  KL  YC+ ED  +
Sbjct: 17  SASPTPQEKGRTCRYAGQAVRALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLV 76

Query: 70  RSNPRNARDPRAQVDDEDVAVVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASR 129
           RSNPRNA+D RAQV+DE+VA++ L+ SDDWVV+LDE+G++I SEQMAEL+ DAGN+GASR
Sbjct: 77  RSNPRNAQDVRAQVEDEEVAMMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASR 136

Query: 130 LAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKYHH 183
           ++FCIGG YGHG Q+R+RANV+I+LSSMVLNHQIAL+VLMEQLY SWTILKGQ YHH
Sbjct: 137 ISFCIGGAYGHGTQVRKRANVTIRLSSMVLNHQIALVVLMEQLYRSWTILKGQNYHH 193

[Close]

BLAST of Cp4.1LG02g04600 vs. TAIR 10
Match: AT5G10605.1 (methyltransferases )

HSP 1 Score: 57.4 bits (137), Expect = 1.4e-08
Identity = 28/36 (77.78%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 131 GGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLME 167
           G  YGHG+QLR+RANV+I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

[Close]

BLAST of Cp4.1LG02g04600 vs. TAIR 10
Match: AT5G10605.2 (methyltransferases )

HSP 1 Score: 57.4 bits (137), Expect = 1.4e-08
Identity = 28/36 (77.78%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 131 GGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLME 167
           G  YGHG+QLR+RANV+I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g04600 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXB4	1.2e-65	71.19	Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
A0M0D0	1.1e-21	37.34	Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0...	[more]
Q1DCX5	5.9e-20	37.50	Ribosomal RNA large subunit methyltransferase H OS=Myxococcus xanthus (strain DK...	[more]
B2V1Q9	1.3e-19	35.26	Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain...	[more]
B1KTH1	1.7e-19	34.62	Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain...	[more]

BLAST of Cp4.1LG02g04600 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023524400.1	3.77e-122	100.00	putative RNA methyltransferase At5g10620 [Cucurbita pepo subsp. pepo]	[more]
XP_022940478.1	1.47e-119	97.80	putative RNA methyltransferase At5g10620 [Cucurbita moschata] >KAG6608193.1 puta...	[more]
XP_022982492.1	8.17e-117	95.60	putative RNA methyltransferase At5g10620 [Cucurbita maxima] >XP_022982493.1 puta...	[more]
XP_008466330.1	2.52e-111	90.66	PREDICTED: putative RNA methyltransferase At5g10620 [Cucumis melo] >XP_008466331...	[more]
XP_011652513.1	1.70e-109	90.11	putative RNA methyltransferase At5g10620 isoform X4 [Cucumis sativus]	[more]

BLAST of Cp4.1LG02g04600 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FPF2	7.13e-120	97.80	putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1IWR3	3.96e-117	95.60	putative RNA methyltransferase At5g10620 OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A1S3CQZ8	1.22e-111	90.66	putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770...	[more]
A0A0A0LGS0	8.22e-110	90.11	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881670 PE=3 SV=1	[more]
A0A6J1BPR4	3.85e-109	88.17	putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=36...	[more]

BLAST of Cp4.1LG02g04600 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10620.1	8.7e-67	71.19	methyltransferases	[more]
AT5G10605.1	1.4e-08	77.78	methyltransferases	[more]
AT5G10605.2	1.4e-08	77.78	methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003742	RNA methyltransferase RlmH	PFAM	PF02590	SPOUT_MTase	coord: 30..181 e-value: 1.3E-44 score: 151.9
IPR003742	RNA methyltransferase RlmH	PIRSF	PIRSF004505	MT_bac	coord: 28..182 e-value: 1.6E-52 score: 175.7
IPR003742	RNA methyltransferase RlmH	PANTHER	PTHR33603	METHYLTRANSFERASE	coord: 9..182
IPR003742	RNA methyltransferase RlmH	HAMAP	MF_00658	23SrRNA_methyltr_H	coord: 28..182 score: 22.643721
IPR003742	RNA methyltransferase RlmH	CDD	cd18081	RlmH-like	coord: 30..182 e-value: 1.4184E-60 score: 183.412
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 29..182 e-value: 6.1E-47 score: 161.2
None	No IPR available	SFLD	SFLDS00070	SPOUT_Methyltransferase	coord: 7..182 e-value: 0.0 score: 250.1
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 30..180

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g04600.1	Cp4.1LG02g04600.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006364	rRNA processing
molecular_function	GO:0008168	methyltransferase activity