Cp4.1LG02g02490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG02g02490
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionProtein VACUOLELESS1
LocationCp4.1LG02: 3769129 .. 3779723 (-)
RNA-Seq ExpressionCp4.1LG02g02490
SyntenyCp4.1LG02g02490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGAGGCTATAAGTGGGCCTAGTTTGGGCCTCATTGAGCCCAATTCAGCCTGTATAGTTGGATCATCAAAATACCGTACGGTTCAAACCGACCTGCGGTCTATATGAACCGGGGGAGCTTCATTCGACAAATATGGGACCTTCACTTTCTTCCATTTTCTTTCTAAATTTTTTTCCCCTTTGCTCGAATTGACTGAATGATCGATTCCAACGTTTTCGAGCCAATTTACGAATCTCAATCTGTAATTCTTCTGTTTGTTCGTTCAATCTGCTTCACGAAGGACTGAAATCTTGAACAATTCTCTAGCTGTGATCGCTTTTTCATGGCTAACGTATCGGTTGCTGCGGAATGGCAGCTCCTTCATAATCGGTACTACCGCAAGCCGGAGCTTTATCCTATGAGATGGAAGCATATTGACCTTGGCCGGAATAAGGTAGCCTGTGCTCCCTTTGGCGGTCCCATCGCCATTATTCGTGATGACTCGAAGATCGTTCAGCTCTACGCAGAATCCGCCCTCAGGAAGCTGCGAATCTTCAACTGTGCAGGTGTTCAGCTGGCAGAGACCGTTTGGCGGAACCCTGGGGGACGGTTGATTGGCATGTCGTGGACCGATGATCAAACTCTTGTCTGTGTGGTGCAGGATGGCACTGTTTACCGCTACAATATTCATGCCGAGCTTCTGGAACCGAATTTTTCGATGGGAAAGGAGTGTTTTGAGCAGAATGTAGTGGAATGTGTGTTTTGGGGCAATGGAGTTGTGTGCATAACCGAGGCCAACCAGATTTTTTGCATCTCGGATTTTAAGAATCCGAAATCGTGTAAGCTTTCGGACCCTGGAATTGAGGATTTGCCGCATTGTATGGCGGTAATCGAGCCTCAATACACCATGTCTGGGAATGTGGAGGTGTTGCTCGGAGTTGGGGAGTCGTGTGTGATAGCTGTTGAGGAGGATGGAGTGCAGCGCCTTGGCGAGGGCGTGCTAGATGGGCCGCTGCAGAGGATGGCCGTCTCTTTAGATGGAAAGTGGTTGGCGGCGTTTACCCACGACGGGAGGCTTTTGGTTTTGACTTCAGACTTGCAGAAAACAATTCTGGATCGTGAGTGTGAGGTTAGCTACGTGTTTTTGCAATTTGAGGTTGTTCTTACGAAATGGATAAAAGTTACATAAGATGCCAGTCCTAGTCTATTTTTTCTGGTCGTACTTGCATAACAACAACTGTTCACTTGAATGTAATGATTCAATGTTCGGGTTTAGATTTTTTTTTTTTTTTCCTTAAGAAGATCGTTTATTTGACATCAAGATGATTCGATGTTTCAAAGCATGGTGGACTAGAACAATTAGTTTATTCAGCCTACAAGCATCCATTCATTTGGCATTTTTCTGAAGTTCTCCCTTTATTCTTGCCTCTTATTCTTCATTTGTCATTTGTCATTTGTTTTTGTGAGATCCCACGTTGGTTGGAGAGGGGAATGAAGCATTCTTTTTAAGGGTGTGGAAACCTCTCCCTAACAGACGCGTTTTAAAACCTCGAGGGGAAGCCTAGAAGGGAAAGTCCAAAGAGGACAATATCTACTGGCGGTGGGCAGAATTGGACATCTATTATGTTTGATCATGTCTGTCTCTTGTTTTCTGGCTGCAGTCAGCTCTTCCTCCGGAGCAGTTAGCTTGGTGTGGAATGGACAGTGTACTTCTTTATTGGGATGATATGCTTTTGATGATGGGTCCAGATGGAGATCCTGTTCGTTACCTTTATGATGAACCGGTTGTTCTGATCCCCGAGTGTGATGGGGTGAGGATATTATCTAATACCAGTACGGAATTTCTTCAAAGAGTACCCGATTCCACCGTAACAATCTTCAGGATCGGAAGTACCTCTCCTGCAGCTCTTCTGTATGATGCATTGGATCATTTTGACAGACGTAGTGCGAAGGTGATGTTAATTTTCCTCTTTCTTGTGAAAAAAATGTAGTGTTAACTAAGCTATTTTTTTCTGTTTTAGATGGTGGAGACTGGCTTAAATTTATTCCATAACTATTGTCGTTTGGTAGTTTTACAATGAGGATAAAAAAGGTCCCTCCTCTAAAATCTATGGGGGAGAGTCTGCCGGGAGAATCGAACCAAAAAAGATAGTTTATGTTTGAGTGAATAGTTATTTTGTCATTTTCCATACTTTATAGCACTAGTAGTATGATGTCCCACATTGGTTAGGGAGGAGAACAAAACACCTTTTATAAGGGTGTGGAAACCTTCCCCTAGCAGACGCGTTTTAAAGCCTTGAGGGGAAACCCGAAAGGGAAAGCCCAAAGAGGAAAATATCTGCTAGCGGTGGATCTGGGTCGTTACAAGTAGTTGTAGTCTTATAAGCTCATTGAAATATTTTTTTCTATTTGTAATGTCTACAAGTGGTATCGGAGGATCTTTTAGAAGGAGGAGCCATTTGATTAGGAAACTATGAAGGAGGAGCTATTTGGTTCGCACTACAAAGGGACGGGGAATGGATTTAACAGACTTATGTTACGATTGGACATTGGCAGCGGGGAATAGATTTAACAAATCAGTGTTATTAATGGACATTGGCATTCTAAGCATACAATAAATAGATATAAATATAAATCATATTTTTCCAGTTTCATTCTCATTTACCAAACACAATCATCCTATTTCTAAGCTTCACATTCTCTTTCCCATTGCCATATACTATATGCCCTCCAAGAGAATTTTCGAGGATAGAGTTACATGAAAGAAAAACTCAGTGCCTGTTTCACAATTGGTCTCAACTATATTTTATTTTCGATATATATATTTTGTAATTTTACTTGGAAGAAATCTCGGTGCTGGTTTTGTAATGGTAAATTTATTTTAGTTTGCAAATTCATGTTCTTGATGATAATATTAGTGTACCCACAAAATACTGTGAAATATTTTCTGTGATTTGTTATTATTCTTTTTATTTAATTGTCAATATTATGTTGGCCAGGCAGATGAAAACTTAAGACTGATACGCCCATCATTACATGAGGCTGTTGAAGCATGCGTGGATGCTGCTGGCCATGAATTTGATATTTCTCGGCAGCAGACCCTGTTAAGAGCTTCCAGCTATGGCCAAGCCTTTTGCAGGTCAGTGATTCTAGTGTTACATTTTAACTACTTTTTTATGATGTTGCTTGTTCTTAGTTCTTTTCTTTTAAATGAGGAAAATTCCGGATGTACTTTCTCTTTTCTTTTGAATTTAAATAGGCACGTGCCTGCCATTTCTTACTTTTTATTTGGTCATAAGATAGTTAAGTGGTAGCTTGTTTAGAATTAAGTATGATGATCCTTCCAAACGAGCACAAATTTAGCTTATTTAACCTTTTTACGTTTGGAATTTGTTGGCAATATGTCATGTGGTTTGAACAATGAGTGCTTCTCTAAGTTTTTTGTTATTATTCTTTACCTCTTTGATTCATGCCCACTGATTACTCCTTTGGGCAGGTTTTGGGAGGATTCCTTATCCTTAACCTCTTACATATTTCACCCTTTTGTGAAATTTGTTTCGTTTAAAAAATATAAAGTTCTTTTTAGTGTTATCTCTGATTTTGCTCTCCATAATGATATCCAGTTGGCTTTGGCAGCAATTTTAATCGCGAACGAATACAAGAGATGTGCAGACTATTGCGAGTTTTAAATGCTGTACGCAACCCAGAGATTGGCATCCCTCTCAGTATACAACAGTTTAAGGTCCTCAATGATTTCTGATAAGAGTTTTCAGTTTCTTGAACTATAATGGATCTTCATACTAAATAAATGTGTTCCTTTCAGCTTCTTACAGCACCTGTTCTGATTGCTCGTTTAATCAATGCCCACCAACACTTGCTTGCATTACGGATTTCTGAGTACCTTGGAATGAGTCAGGTGAATAGAATCCTATAATTCGTGGATCAAACATATGGTGATTTTTCTTGTTGCTTGTGTTTTTATACGACACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCACACACACACACACACACACGCACACAGCTGTGAAAACTTTGAAATTTACATGGAAATTATGTTGTAGAATGACTCATGATAAAATGAATTGTTTCCTTGGTTCTTGCAATGATAATAAATTGAAAATAAAGATAATATTATTACCCGAATAATTTTCATTTAAGAAGCAGAGTACTTTTACTTAAGAGACAGTGAGGAATAAGCATTAAAATTTTCATTTTTCAAATGTCTATTAAGAAGAATCATCAAATGAAGAATTGAATTTACATTTGAGCATAAGATCGGAAGCTACTTCCTGCTCATGGTTAACCCCGCTTTTGTGGTTGAAGGTGCTTTGAGATTCTGACTTTTAAGCAACTTTTGCCTATCGATAAAAGATAAAATAAGTCTAAATTAGAAGAAATGAGGCCGATAAAGAAGAAAATAAATGTGTACTTCGGGAACTTGCAATTTTAGGAAGTGTAAGATACATCCTAATAAGATTTTGAAGTTGTTACAGCTGCAAAAAAAAGAAATTTCATTGCTCCATTTGATATCATGTCTCCCTCATTGACCCAATTCAACGAAAATCCAAGCACTGAGTACTTAGAATATGTCTACCATTTATCGGCTTGTTGCTTATTCTCTCAACAAGATAAATATATTTTGTATCTGTTATACAGGAGGTGGTGATAATGCACTGGGCATGTTCAAAGATAACAGCTTCATTAAATATTCCTGATGTCACCCTTCTTGAAGTTCTACTTGATAAGGTTTTATATTTTTCATACATCAAGTATTGAGATTCTGTTCCTTTAGTATCATGCAATGGCGTCTGAAATGAACTCTTGGGAAATTCAGCTGAAGTTGTGTAAAGGCATATCATATGCTGCCGTTGCTGGTCATGCTGATAAAATTGGTCGCAGGAAATTAGCTGCCATGCTTGTTGATCATGAACCGCGGTCCTCCAAACAGGTCTTTTTTTCTTTACATGTCATTTCTTCTTCTTGGATTCTGTTATTTTTGGGCATAAAAACATATAAATTAAGAGAAGATCTACGAGTATCCTGTAGTATACGAGTTGTCTTTTTGTTTTTCCATCAGTATATCTCCAGATTTTTTATTAAGCAAAAAAAAAAAAAACATACNGCCATACAACATTAAACCGAAAAAGGCCCAGGAGATTGCAAAAGCTCTCCAATTTGCTTCTTTTTCTTTCTATAATTGCATTTTTGTAGCAAAGGTAATAAGTAGAAATACTTTTTAGTAAAAATACCAGTTCAAAACATAGAAAATCTCAGCTGCTTTCAATCCTATGTGTTCGTTTCCTTTCTCCTTTTTTCTTTTAAAAATGTCTTCTTTTCCTCTCATTTCATTTCCTTTATCATTATTTTTTCTTTTAAATTATTTTCCTCTCTTTCTGCAAATTCCACAAGGCTGTTGGGATTGCAAATGTTCACGTGTTCCTCGCTCTTCTTATGAACTTTGGGCCAAAAGGAAGCTGGAAAGAAAATCCCTTAGTTTCACAGGGACAATACCCATCCAAGGTTGGGCTGTTCAAATATCCCAATGCCCTGGAAAGCTGAATAGCAGAACCCAAACCACCCAAAATGTACTCTCCTCTCTTCTCTCTCACACACACAAACTCGGGCTGATGCATGCTAAGCATCAGAACCTCCATGGATGCATCAACGTTTTCTCTAAAATGCAGCAGTTAATAGAAGTAATTGGTGGTTCCATCAAAATCTTGTTGTAGTTTTACCATGTCTAGGTCTGCTGTTTCAGAAATGAGAGTGCTTACTTTGGCAAACAAAGCTTGTTTGGTTGGACAGTGCAGAAGAACTCTCTTGGTGTTTTGAAGTATGAACCTAAAGAGAGGTCAGATGTGTCAAAATGCACCAAATTCCTGAACTCCTCCAATAACCTTCTTCTACTCAGAGTCCCAGAAAGCCTCACTACTGGGATTTACCCACAAAGGAGGGTCCAAAATATCCTTATGGCCATTTACTAACCAAATGTGATAGAGCTTCTTCATTCAATTTTTGATCTTTTGCAAATCGGTAATGGAATTTCATGATGTCAGCTTTTGGATAGAGTACAGCGTTGCCTGGGAATATTTTTGACTTCCTATCTCCGATTTTTGTGGTACATCCTTTCAAAGGAGATGCAAATGCTCTTTGGATGGCCTTCAACCGTTCCTTCTTTTGGTCTCTTTGGCATGAAAGGAATGAGTGGTACTTCAACGACAATTCTTGGTCTTTTGAGGGGTTATTTGATTTAGTTCTTTCAATACTTTATTTTGGTGTAAAAGTTCACACCCCTTTAGAGATTAGTCTTACTGCCTTATTAAACATATGCATTTCTTTCTTGTTACTCACTTTTGTGGGCTATTCATTTCATTTATCAAGGAAATCTCTCCGTTTCTCTTCCAAAAAAAAAAAAAGAAAAAAACTTCACTTCGGTGTATTCAATACTGGCAATGTAATTTTTGAACATGCATTTTCAAACTACGAGACTGTTTCAATCAGGTAGAGAATTTTCAAACGGACATGATTTTTTTCTTTTGTTGTTGATGCTATCATATCCTTTCAATGTATGTCGATACATTTTTGTTATACTATGGAAGTGGAACTGTAGATGAAAGAGAAATAAATCATTATTATGAAAATATTTATCATGAACTCGTTTCCTTTTTTTCACTTTGTCCATTTAGGTACCTCTCTTGTTAAGCATAGGAGAAGAAGACACTGCATTAATCAAAGCAACTGAAAGTGGTGATACTGATCTTGTTTATCTTGTTCTCTTCCATATCTGGCAAAAGGTAATTCTTCAATAATATCTAATATATGTATGATTTCTTATCACACGTCTGCATATTTTATGAGTCTAAGATATGCATAAATAAGATCTAAGTGGTATTTACAACTTTTAAATAGCATGCATGTTCTTAACGAAAGCTTGTTTTTTTCCATAAAAAGAGAACTTTTGAATAGAATGCATAATTGGCTTTTATGCAGAAGCAACCACTGGAATTCTTTGGAATGATACAAGCCAGAATTCAGGCACGGGATTTATTTGTTACTTATGCCCGGTATGTATGAACATTTCTATTCCTCCAGTAATAGATTTGATGCCAAAAGCTTAATACTTTATTTTAAGATTGCAGATTCTTCAATTATTACTTTGAGATTAATTTTGCCTCTATTTAATCTATATGAGTGAGATTTTCTTTGTTTTTATTCAGTCTTTTAACTTGAGGATTTCTCGTCCTCCACTGTATTTGTCTATATTTTCATTTGATGTAAACTGTCTTCCATTAAAATCCATGAGATAAAATAAGAAGTCGATGGAACCAACTCTTGTAAATTGTTGACGAATTAAAAGCTTATGTAGGCTGCAATGGAGATATCATTTTTCTTCTTTGTATGCTTACACTAATGTGTTGGAGAGCTGTTTGGTTTCCATTTATCGTAATTCTTTGGGGTTACATAAAAAATTTGTCAGGTTCCACTGTATCGGAAGGAAAATAAACTGAATATAATATTTTCAGACTGCTCGCAGAGCTCGTAGTTGCAGATAATAAAAAATAGCATGGATGCAGATGTTCATTATGACACTACAAATATCATACTCTTGATCTACCAAACTTCTAGTGTTTATTTAGTGGTTTGCTGGTTATTAATTGTATGGCTTAATGGAAGCATAGACTCATGACATCGTCTCATGTTTGGTTCTTGAGCTCATCTCTTGTTTTAAAATGCACTTGTACTTAGACATGACCTTTTTATTTGCTTGACTGATTATGTTATCTCCTTCAGGTGCTATAAGCATGAATTTCTGAAGGACTTTTTCCTATCAACTGGACAACTTCATGTACGTCTTTATGTTCAATATTGGTCGCATCTTTATTTTTTTCAATTTATATGTTGAAACGTATCTCACGAGTTCGTACTTCTTTTTTATATGGATTAGAGAAACGGTAGGTCTGTTATGTGGAGTATATCTTTTATTTGGAATGATAAGTTTCTTCGTTTCTTCGGAGGAAAAGAAATTACACCAAAATGGTCTTTTTGGATCTGATCGGCCATGTTGATACCCCCGTGTCTGAGACTGAGGACCAAATAGAAAACCTTAAATCTTTGAATATTGGCGCTTTCACATAGTCAAAAGCTCAAAAGTGTATTTAGAATTTCATCCTTGTCCTGAGTTCCGAGATGAATAAAAGGGTAATAGGTGGAAAATCCCGAGTCTCTTAAGAACTCAAATTCTCCTATCCTCTTTGTGGCTAGGACATCATTTTTTCTTTCTTTAAACCACGCAAGACGCACCTTCGATGAGATAAGACAGTTAAAGATGAACCACAATTTTTTATTGGCATTAATCTTTCTGTGATGACACTTCAAGGGCCATTCTCCTATACCCTTTCTAATACAGTTTATTTATTTTTATAGGAGGTTGCTTTTCTTCTCTGGAAAGAGTCGTGGGTGCTAGGGAAAAATCCAATGGCTAGCAAGGGATCTCCACTCCATAGTCCACGCATTAAACTCATTGAAAAGGTCCAGAGTCTTTTTGCAGAGACCAAGGAACATGTTTTCGAGTCAAAAGCTGCTGAAGAGCATGCAAAATTGTTGAAGTTAGTATAAGCTTGGTTACTTTGTGTTTGTGAATATAAAAAGACATAACACCACTAAATCATTCTCTGATCTCGTCTGCAGAATACAACATGATCTAGAGGTAAGTACGAAGCAGGCCATTTTTGTTGATTCAAGTATTAATGATACAATTCGAACATGTATTGTGTTGGGAAATCATCGAGCTGCTCTAAAAGTTAAAACAGAATTCAAGGTATGTGAACTTCAATTTGCTATTTCCCTCCGTGTATGGTATTGTAGGATAATTACTTTGTTCATGTGTTCCTATTCATATCTCCCAGCTGTTTTTTTCATGTTGTGCCTCAGGTTTCTGAGAAAAGATGGTATTGGCTTAAAGTTTTTGCTTTGGCTACCACAAGAGATTGGGTCGCATTGGAAACGTTCTCGAAGGAGAAAAGACCGCCAATCGGTAAATGTCCGATTATTAGAGTAGCAAATTACTTTCTCCTATTAGATTGACATCTATTTACATGCTTACTTATTCTGCCTTGAAATGAGATGCAAACCGTTCCTGAGATTCATTGAATATCTTTTATCTTAACGAATATCTTTTTGGAAAGCACTTACGCACAAAGGTGCTTCCATGAACAAGAGTGAGGAATTACTTATGGGTTCTTCACCAACTCACTCTCATTTCAATCATGCTCGTTAGGTTGTAGCTACTTGTATTTTAACAGTCGGTACATGATTTCATGTGTAAACTTGTGATTAGGCTTGTAACTCTTTCCGTTCATGTTTTATAAAAGAAAAACAAAAATTACTTGCGACTTTTTTAATATATTTCAGTGCTTCAACCTTAATTAGAATTGTAATATTCAGTTAGGCTCTTTATCATTAATATCTCTTTCGTTTTTTTCTTTTATTAACAGGTTACAAGCCATTTGTTGAGGCTTGCATTGAAGCAGATGAAAAGGCTGAAGCACTGAAATATATTCCAAAACTTGCAGATCCACGAGAAAGGGCAGAGGTAATATAACATTCTAACCCATGCATACTATAGCTTCAATTTTTTGCTTGTATTGCTGGCTGGTAGTTTCATAAATGGGAATTTACGATCTTACCAAAGATGAAAAGTTTAGCATTCAAATCATGAAATTTCCAAATTGTGGCACTTAAAAAACCAGATTTATCTCTATTGAATCAAGTTTATTCAACATAATCACTGGTCGAGCAGCTATCTAGCAACATGTGCAGATTGTGAAAGTGTGATATTTATTTTCTTTACAGGCTTATGCTCGGATTGGCATGGCCAAGGAAGCAGCCGACGCTGCTTCACAAGCGAAGGATGGAGAATTACTAGGTCGATTAAAATTAACCTTTGCACAAAATTCAGCAGCGTCATCAATTTTCGATACTCTTCGAGATCGGTTGTCCTTCCCAGGAGTGTCATAGTCTTTTCAGTCCCACGTTTCTTTGCTCTATTATCTTATTCGGTTTCCCTCCTAAGCTTCTGCTGGTGTTTTTTGTGAAATATTTAGCTATGATTGGTGCATACCCATATGTTGAAATATGTCACTGATTTGGACTGTTGTATTCGTTAATTATTTCTGACCCTTGTAACTTTGGCTTTTCCTTCCTCTCTAAACCATGTTCTTTGTCGTCTGTTTGTATAATTACCCCAAGCAACTAACAGTTCAGGGAGACGTGTTCCTATGTTACCTAATTGCCATCAAAATATTCTGTTTTGTTGATATACTGAAATGTGATGGAAAATTACCACAAAAAAAAGAGAAAAAAAAAAGAATCCTAGGATGGTTGAATCTCATGTACATGGTCCGGG

mRNA sequence

AAGAGGCTATAAGTGGGCCTAGTTTGGGCCTCATTGAGCCCAATTCAGCCTGTATAGTTGGATCATCAAAATACCGTACGGTTCAAACCGACCTGCGGTCTATATGAACCGGGGGAGCTTCATTCGACAAATATGGGACCTTCACTTTCTTCCATTTTCTTTCTAAATTTTTTTCCCCTTTGCTCGAATTGACTGAATGATCGATTCCAACGTTTTCGAGCCAATTTACGAATCTCAATCTGTAATTCTTCTGTTTGTTCGTTCAATCTGCTTCACGAAGGACTGAAATCTTGAACAATTCTCTAGCTGTGATCGCTTTTTCATGGCTAACGTATCGGTTGCTGCGGAATGGCAGCTCCTTCATAATCGGTACTACCGCAAGCCGGAGCTTTATCCTATGAGATGGAAGCATATTGACCTTGGCCGGAATAAGGTAGCCTGTGCTCCCTTTGGCGGTCCCATCGCCATTATTCGTGATGACTCGAAGATCGTTCAGCTCTACGCAGAATCCGCCCTCAGGAAGCTGCGAATCTTCAACTGTGCAGGTGTTCAGCTGGCAGAGACCGTTTGGCGGAACCCTGGGGGACGGTTGATTGGCATGTCGTGGACCGATGATCAAACTCTTGTCTGTGTGGTGCAGGATGGCACTGTTTACCGCTACAATATTCATGCCGAGCTTCTGGAACCGAATTTTTCGATGGGAAAGGAGTGTTTTGAGCAGAATGTAGTGGAATGTGTGTTTTGGGGCAATGGAGTTGTGTGCATAACCGAGGCCAACCAGATTTTTTGCATCTCGGATTTTAAGAATCCGAAATCGTGTAAGCTTTCGGACCCTGGAATTGAGGATTTGCCGCATTGTATGGCGGTAATCGAGCCTCAATACACCATGTCTGGGAATGTGGAGGTGTTGCTCGGAGTTGGGGAGTCGTGTGTGATAGCTGTTGAGGAGGATGGAGTGCAGCGCCTTGGCGAGGGCGTGCTAGATGGGCCGCTGCAGAGGATGGCCGTCTCTTTAGATGGAAAGTGGTTGGCGGCGTTTACCCACGACGGGAGGCTTTTGGTTTTGACTTCAGACTTGCAGAAAACAATTCTGGATCGTGAGTGTGAGGCAGATGAAAACTTAAGACTGATACGCCCATCATTACATGAGGCTGTTGAAGCATGCGTGGATGCTGCTGGCCATGAATTTGATATTTCTCGGCAGCAGACCCTGTTAAGAGCTTCCAGCTATGGCCAAGCCTTTTGCAGCAATTTTAATCGCGAACGAATACAAGAGATGTGCAGACTATTGCGAGTTTTAAATGCTGTACGCAACCCAGAGATTGGCATCCCTCTCAGTATACAACAGTTTAAGCTTCTTACAGCACCTGTTCTGATTGCTCGTTTAATCAATGCCCACCAACACTTGCTTGCATTACGGATTTCTGAGTACCTTGGAATGAGTCAGGAGGTGGTGATAATGCACTGGGCATGTTCAAAGATAACAGCTTCATTAAATATTCCTGATGTCACCCTTCTTGAAGTTCTACTTGATAAGCTGAAGTTGTGTAAAGGCATATCATATGCTGCCGTTGCTGGTCATGCTGATAAAATTGGTCGCAGGAAATTAGCTGCCATGCTTGTTGATCATGAACCGCGGTCCTCCAAACAGGTACCTCTCTTGTTAAGCATAGGAGAAGAAGACACTGCATTAATCAAAGCAACTGAAAGTGGTGATACTGATCTTGTTTATCTTGTTCTCTTCCATATCTGGCAAAAGAAGCAACCACTGGAATTCTTTGGAATGATACAAGCCAGAATTCAGGCACGGGATTTATTTGTTACTTATGCCCGGTGCTATAAGCATGAATTTCTGAAGGACTTTTTCCTATCAACTGGACAACTTCATGAGGTTGCTTTTCTTCTCTGGAAAGAGTCGTGGGTGCTAGGGAAAAATCCAATGGCTAGCAAGGGATCTCCACTCCATAGTCCACGCATTAAACTCATTGAAAAGGTCCAGAGTCTTTTTGCAGAGACCAAGGAACATGTTTTCGAGTCAAAAGCTGCTGAAGAGCATGCAAAATTGTTGAAAATACAACATGATCTAGAGGTAAGTACGAAGCAGGCCATTTTTGTTGATTCAAGTATTAATGATACAATTCGAACATGTATTGTGTTGGGAAATCATCGAGCTGCTCTAAAAGTTAAAACAGAATTCAAGGTTTCTGAGAAAAGATGGTATTGGCTTAAAGTTTTTGCTTTGGCTACCACAAGAGATTGGGTCGCATTGGAAACGTTCTCGAAGGAGAAAAGACCGCCAATCGGTTACAAGCCATTTGTTGAGGCTTGCATTGAAGCAGATGAAAAGGCTGAAGCACTGAAATATATTCCAAAACTTGCAGATCCACGAGAAAGGGCAGAGGCTTATGCTCGGATTGGCATGGCCAAGGAAGCAGCCGACGCTGCTTCACAAGCGAAGGATGGAGAATTACTAGGTCGATTAAAATTAACCTTTGCACAAAATTCAGCAGCGTCATCAATTTTCGATACTCTTCGAGATCGGTTGTCCTTCCCAGGAGTGTCATAGTCTTTTCAGTCCCACGTTTCTTTGCTCTATTATCTTATTCGGTTTCCCTCCTAAGCTTCTGCTGGTGTTTTTTGTGAAATATTTAGCTATGATTGGTGCATACCCATATGTTGAAATATGTCACTGATTTGGACTGTTGTATTCGTTAATTATTTCTGACCCTTGTAACTTTGGCTTTTCCTTCCTCTCTAAACCATGTTCTTTGTCGTCTGTTTGTATAATTACCCCAAGCAACTAACAGTTCAGGGAGACGTGTTCCTATGTTACCTAATTGCCATCAAAATATTCTGTTTTGTTGATATACTGAAATGTGATGGAAAATTACCACAAAAAAAAGAGAAAAAAAAAAGAATCCTAGGATGGTTGAATCTCATGTACATGGTCCGGG

Coding sequence (CDS)

ATGGCTAACGTATCGGTTGCTGCGGAATGGCAGCTCCTTCATAATCGGTACTACCGCAAGCCGGAGCTTTATCCTATGAGATGGAAGCATATTGACCTTGGCCGGAATAAGGTAGCCTGTGCTCCCTTTGGCGGTCCCATCGCCATTATTCGTGATGACTCGAAGATCGTTCAGCTCTACGCAGAATCCGCCCTCAGGAAGCTGCGAATCTTCAACTGTGCAGGTGTTCAGCTGGCAGAGACCGTTTGGCGGAACCCTGGGGGACGGTTGATTGGCATGTCGTGGACCGATGATCAAACTCTTGTCTGTGTGGTGCAGGATGGCACTGTTTACCGCTACAATATTCATGCCGAGCTTCTGGAACCGAATTTTTCGATGGGAAAGGAGTGTTTTGAGCAGAATGTAGTGGAATGTGTGTTTTGGGGCAATGGAGTTGTGTGCATAACCGAGGCCAACCAGATTTTTTGCATCTCGGATTTTAAGAATCCGAAATCGTGTAAGCTTTCGGACCCTGGAATTGAGGATTTGCCGCATTGTATGGCGGTAATCGAGCCTCAATACACCATGTCTGGGAATGTGGAGGTGTTGCTCGGAGTTGGGGAGTCGTGTGTGATAGCTGTTGAGGAGGATGGAGTGCAGCGCCTTGGCGAGGGCGTGCTAGATGGGCCGCTGCAGAGGATGGCCGTCTCTTTAGATGGAAAGTGGTTGGCGGCGTTTACCCACGACGGGAGGCTTTTGGTTTTGACTTCAGACTTGCAGAAAACAATTCTGGATCGTGAGTGTGAGGCAGATGAAAACTTAAGACTGATACGCCCATCATTACATGAGGCTGTTGAAGCATGCGTGGATGCTGCTGGCCATGAATTTGATATTTCTCGGCAGCAGACCCTGTTAAGAGCTTCCAGCTATGGCCAAGCCTTTTGCAGCAATTTTAATCGCGAACGAATACAAGAGATGTGCAGACTATTGCGAGTTTTAAATGCTGTACGCAACCCAGAGATTGGCATCCCTCTCAGTATACAACAGTTTAAGCTTCTTACAGCACCTGTTCTGATTGCTCGTTTAATCAATGCCCACCAACACTTGCTTGCATTACGGATTTCTGAGTACCTTGGAATGAGTCAGGAGGTGGTGATAATGCACTGGGCATGTTCAAAGATAACAGCTTCATTAAATATTCCTGATGTCACCCTTCTTGAAGTTCTACTTGATAAGCTGAAGTTGTGTAAAGGCATATCATATGCTGCCGTTGCTGGTCATGCTGATAAAATTGGTCGCAGGAAATTAGCTGCCATGCTTGTTGATCATGAACCGCGGTCCTCCAAACAGGTACCTCTCTTGTTAAGCATAGGAGAAGAAGACACTGCATTAATCAAAGCAACTGAAAGTGGTGATACTGATCTTGTTTATCTTGTTCTCTTCCATATCTGGCAAAAGAAGCAACCACTGGAATTCTTTGGAATGATACAAGCCAGAATTCAGGCACGGGATTTATTTGTTACTTATGCCCGGTGCTATAAGCATGAATTTCTGAAGGACTTTTTCCTATCAACTGGACAACTTCATGAGGTTGCTTTTCTTCTCTGGAAAGAGTCGTGGGTGCTAGGGAAAAATCCAATGGCTAGCAAGGGATCTCCACTCCATAGTCCACGCATTAAACTCATTGAAAAGGTCCAGAGTCTTTTTGCAGAGACCAAGGAACATGTTTTCGAGTCAAAAGCTGCTGAAGAGCATGCAAAATTGTTGAAAATACAACATGATCTAGAGGTAAGTACGAAGCAGGCCATTTTTGTTGATTCAAGTATTAATGATACAATTCGAACATGTATTGTGTTGGGAAATCATCGAGCTGCTCTAAAAGTTAAAACAGAATTCAAGGTTTCTGAGAAAAGATGGTATTGGCTTAAAGTTTTTGCTTTGGCTACCACAAGAGATTGGGTCGCATTGGAAACGTTCTCGAAGGAGAAAAGACCGCCAATCGGTTACAAGCCATTTGTTGAGGCTTGCATTGAAGCAGATGAAAAGGCTGAAGCACTGAAATATATTCCAAAACTTGCAGATCCACGAGAAAGGGCAGAGGCTTATGCTCGGATTGGCATGGCCAAGGAAGCAGCCGACGCTGCTTCACAAGCGAAGGATGGAGAATTACTAGGTCGATTAAAATTAACCTTTGCACAAAATTCAGCAGCGTCATCAATTTTCGATACTCTTCGAGATCGGTTGTCCTTCCCAGGAGTGTCATAG

Protein sequence

MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCMAVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFTHDGRLLVLTSDLQKTILDRECEADENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFAETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIPKLADPRERAEAYARIGMAKEAADAASQAKDGELLGRLKLTFAQNSAASSIFDTLRDRLSFPGVS
Homology
BLAST of Cp4.1LG02g02490 vs. ExPASy Swiss-Prot
Match: Q93VQ0 (Protein VACUOLELESS1 OS=Arabidopsis thaliana OX=3702 GN=VCL1 PE=1 SV=1)

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 572/865 (66.13%), Postives = 653/865 (75.49%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLL++RYYRKPE+Y M+WKH+DL RNKVACA FGGPIA+IRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLYDRYYRKPEIYQMKWKHVDLSRNKVACASFGGPIAVIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFN AG+ L+ETVW++PGGRLIGMSW+DDQTL+C+VQDGT+YRYNIHAEL+
Sbjct: 61  AESALRKLRIFNSAGILLSETVWKHPGGRLIGMSWSDDQTLICIVQDGTIYRYNIHAELI 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSD-PGI--EDL- 180
           EPN SMG+ECFEQNVVECVFWGNGVVC+TE  Q+ CI DFK  K  KL D PG+  +DL 
Sbjct: 121 EPNMSMGQECFEQNVVECVFWGNGVVCLTEGGQLICIFDFKTMKPSKLPDVPGLAEDDLL 180

Query: 181 -PHCMAVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRL---------------GEGVL 240
            P C+ V EP+YTMSG  EVL+ VG+  +  VEED VQ +                 G L
Sbjct: 181 QPICLTVREPKYTMSGIAEVLVAVGDD-IFVVEEDMVQTIRFDEPSVDDSEMQNDDSGNL 240

Query: 241 DGPLQRMAVSLDGKWLAAFTHDGRLLVLTSDLQKTILDRECE------------------ 300
            G +Q+M VS +GK+L  FTHDGR++V+  + ++  +D  CE                  
Sbjct: 241 IGVVQKMIVSPNGKFLTLFTHDGRIVVVDMETKQIAIDYSCESALPPQQMAWCGMDSVLL 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 YWDEDLMMVGPVGDPVHYFYDEPIILIPECDGVRILSNTNLEFLQRVPDSTESIFKIGST 360

Query: 361 -------------------ADENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSY 420
                              ADENLRLIR SL EAVE+C+DAAGHEFD++RQ+ LLRA+SY
Sbjct: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLSEAVESCIDAAGHEFDVTRQRALLRAASY 420

Query: 421 GQAFCSNFNRERIQEMCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLL 480
           GQAFCSNF RER+QE CR LRVLNAVR+P IGIPLSIQQ+KLLT  VLI+RLINA+ HLL
Sbjct: 421 GQAFCSNFQRERVQETCRTLRVLNAVRDPAIGIPLSIQQYKLLTPVVLISRLINANCHLL 480

Query: 481 ALRISEYLGMSQEVVIMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADK 540
           ALRISEYL M++EVVIMHWAC+KITAS + PD  LLE+LLDKL+LCKGISYAAVA HAD 
Sbjct: 481 ALRISEYLDMNKEVVIMHWACAKITASPSTPDSHLLEILLDKLQLCKGISYAAVATHADN 540

Query: 541 IGRRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPL 600
            GRRKLAAMLV+HEPRS+KQVPLLLSIGEEDTAL+KATESGDTDLVYLV+FHIWQK+ PL
Sbjct: 541 CGRRKLAAMLVEHEPRSTKQVPLLLSIGEEDTALVKATESGDTDLVYLVIFHIWQKRPPL 600

Query: 601 EFFGMIQARIQARDLFVTYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASK 660
           EFF MIQ R+ ARDLFV YARC+KHEFLKDFFLSTGQ+HEVAFLLWKESW +GKNPMASK
Sbjct: 601 EFFAMIQGRVLARDLFVAYARCHKHEFLKDFFLSTGQIHEVAFLLWKESWDMGKNPMASK 660

Query: 661 GSPLHSPRIKLIEKVQSLFAETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSIN 720
           GSPLH PRIKLIEK ++LF++TKEH FESKAAEEHAKLLKIQH+LE STKQAIFVDSSIN
Sbjct: 661 GSPLHGPRIKLIEKARNLFSQTKEHTFESKAAEEHAKLLKIQHELEASTKQAIFVDSSIN 720

Query: 721 DTIRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYK 748
           DTIRTCIVLGN+RAA+KVKTEFKVS+KRWYWLK FALAT +DW ALE FSKEKRPP+G++
Sbjct: 721 DTIRTCIVLGNNRAAIKVKTEFKVSDKRWYWLKAFALATIKDWAALEKFSKEKRPPMGFR 780

BLAST of Cp4.1LG02g02490 vs. ExPASy Swiss-Prot
Match: Q55C58 (Vacuolar protein sorting-associated protein 16 homolog OS=Dictyostelium discoideum OX=44689 GN=vps16 PE=3 SV=2)

HSP 1 Score: 419.5 bits (1077), Expect = 8.1e-116
Identity = 272/839 (32.42%), Postives = 435/839 (51.85%), Query Frame = 0

Query: 6   VAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 65
           +AA+W+++ N  Y K E+Y M W  +DL +     +PF GPIA++RD SK V++ +++  
Sbjct: 2   IAAQWKIIGNSTYIKKEIYSMSW-DVDLKQQVSVGSPFAGPIAVMRDSSKFVEMNSQNMK 61

Query: 66  RKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 125
             L+IF  +G  +++ +W +    ++ M W + + LV V+Q+ TV  +N+  E +   FS
Sbjct: 62  PYLKIFTASGDLISQMIW-DSSKNIVAMDWIEKERLVIVLQNATVLIFNVFCEQM-TQFS 121

Query: 126 MGKECFEQNVVECVFWGNGVVCITEANQIF---CISDFKNPKSCKLSDPGIEDLPHC--- 185
           +G    E+ ++EC  W +G+V +T A+Q++    I+DF       +  P + + P     
Sbjct: 122 LGDIVREEEILECKIWSDGIVVLTSASQLYSVPSINDFFVESGRVIRLPPLPEEPKARPE 181

Query: 186 MAVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAF 245
            A++EPQ+++S ++E+ + +  +  + ++ED V+   E     P+Q+M VS  GK LA F
Sbjct: 182 WAILEPQFSLSQSIEIFMSINGTLYL-IDEDKVESQLEAT--EPIQKMVVSPCGKKLACF 241

Query: 246 THDGRLLVLTSDLQKTILDR---------------------------------------- 305
              G LL+L +D   T  DR                                        
Sbjct: 242 DTKGTLLILKTDGSTTNPDRMDTKATKSPVLKWCGSDGVMMYWDSIKDPILFYFSKGDSW 301

Query: 306 ------------------------------------------------------------ 365
                                                                       
Sbjct: 302 AKFTLDQPVSLVTEIDGLRIISDTTSEFFHKVSDVTIDIFKIGTTSPASILYDATDHFIS 361

Query: 366 -ECEADENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQE 425
              +ADE++R I   L +AV  C+ AAG EF+   Q  LL+A+S+G+ F  N+N  +   
Sbjct: 362 KSPQADESIRSINDQLEDAVNDCILAAGFEFNGGEQSKLLKAASFGKCFLENYNPSQFVT 421

Query: 426 MCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVV 485
           MCR LRVLNAVR+ EIGIPLSI+Q+  +    LI RLI+  +HLLA RI +YL +  +VV
Sbjct: 422 MCRSLRVLNAVRHHEIGIPLSIKQYYHIGIEELIDRLISRRKHLLAWRICDYLKIKSDVV 481

Query: 486 IMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEP 545
           + HWAC+K+    +IPD  L ++++ KL+   GIS+A +A  A   GR KLA  L+++EP
Sbjct: 482 LNHWACTKVRT--DIPDQELGKIIIKKLESVPGISFANIASAAYLAGRSKLATKLLEYEP 541

Query: 546 RSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDL 605
           ++++QVP L+ +GE   AL KA ESGDTDLVYLVL  + +     +F  +  +++ A DL
Sbjct: 542 KAAEQVPPLIKMGESGLALNKAIESGDTDLVYLVLLAMQRSLPLADFLELTFSKVVALDL 601

Query: 606 FVTYARCYKHEF--LKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIE 665
            ++  +  K++F  L++ +    Q  E+  +  +E+       ++S  S L   RIK   
Sbjct: 602 LISMCK-QKNDFPLLREIYHIKDQSKEMGNIYLQEA-------LSSHPSQL-DQRIKAYN 661

Query: 666 KVQSLF--AETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGN 725
           K    +  ++ K+    SK  ++  KL  +Q +LE + +   FV  SINDTI   I +  
Sbjct: 662 KSIEHYHHSKDKDDQATSKFIDDQIKLEMLQKELETNLQDESFVGISINDTIYKLITMNQ 721

Query: 726 HRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADE 734
            + A  +++EFKV +KR++W+K+ AL+   DW  L  FSKEK+ PIGY+PFVE C++   
Sbjct: 722 PKKAQSIRSEFKVPDKRFWWIKIKALSIMNDWEELMKFSKEKKSPIGYEPFVEVCLDQKN 781

BLAST of Cp4.1LG02g02490 vs. ExPASy Swiss-Prot
Match: Q9H269 (Vacuolar protein sorting-associated protein 16 homolog OS=Homo sapiens OX=9606 GN=VPS16 PE=1 SV=2)

HSP 1 Score: 323.2 bits (827), Expect = 7.9e-87
Identity = 245/828 (29.59%), Postives = 394/828 (47.58%), Query Frame = 0

Query: 8   AEWQLL-HNRYYRKPELYPMRWK-HIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 67
           A W  L  + +YRK ELY M W    +L    VA AP+GGPIA++R+  +  +  A S  
Sbjct: 6   ANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWR--KEKAASVR 65

Query: 68  RKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 127
             L I++ +G+ LA  +W++  G ++ + W+ ++ L+CV +DG V  Y +H +    +FS
Sbjct: 66  PVLDIYSASGMPLASLLWKS--GPVVSLGWSAEEELLCVQEDGAVLVYGLHGD-FRRHFS 125

Query: 128 MGKECFEQNVVEC-VF---WGNGVVCITEANQIFCISDFKNPKSCKLSD-PGIEDLPHCM 187
           MG E  +  V++  +F   +G+GV  +T A++    ++  + K  ++ + PG++  P C 
Sbjct: 126 MGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLQSAPSCW 185

Query: 188 AVIEPQYTMSGNVEVLLGVGESCVI-------AVEEDGVQRLGEGVLDGPLQRMAVSLDG 247
            V+           +LL VG    +       AV   G   L  GV      +MAVS   
Sbjct: 186 TVL----CQDRVAHILLAVGPDLYLLDHAACSAVTPPG---LAPGV--SSFLQMAVSFTY 245

Query: 248 KWLAAFTHDGRLLVLTSDLQKTILDREC-------------------------------- 307
           + LA FT  G + + T+ L++ + +  C                                
Sbjct: 246 RHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMV 305

Query: 308 ------------------------------------------------------------ 367
                                                                       
Sbjct: 306 VGDAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPAASEEIFKIASMAPGALLLE 365

Query: 368 ----------EADENLRLIRP--SLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCS 427
                     +ADE LR I+    L +AV+ C++AAGHE     Q++LLRA+S+G+ F  
Sbjct: 366 AQKEYEKESQKADEYLREIQELGQLTQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLD 425

Query: 428 NFNRERIQEMCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISE 487
            F  +    MC+ LRVLNAVR+  IGIPL+  Q+K LT  VL+ RL+    + LA++I E
Sbjct: 426 RFPPDSFVHMCQDLRVLNAVRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICE 485

Query: 488 YLGMSQ----EVVIMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIG 547
           YL + +      ++ HWAC K+    ++ D  +   +  KL    G+SY+ +A  A   G
Sbjct: 486 YLRLPEVQGVSRILAHWACYKVQQK-DVSDEDVARAINQKLGDTPGVSYSDIAARAYGCG 545

Query: 548 RRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEF 607
           R +LA  L+++EPRS +QVPLLL +     AL KA ESGDTDLV+ VL H+  +    +F
Sbjct: 546 RTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDF 605

Query: 608 FGMIQARIQARDLFVTYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGS 667
           F  ++ +  A  L+  + +  + E LKD +       E+     + S+   +        
Sbjct: 606 FMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEER------- 665

Query: 668 PLHSPRIKLIEKVQSLFAETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDT 714
                R+  ++     F + K   F +KA E+  +LL++Q  LE       F+D S++DT
Sbjct: 666 --IEGRVAALQTAADAFYKAKNE-FAAKATEDQMRLLRLQRRLE-DELGGQFLDLSLHDT 725

BLAST of Cp4.1LG02g02490 vs. ExPASy Swiss-Prot
Match: Q5E9L7 (Vacuolar protein sorting-associated protein 16 homolog OS=Bos taurus OX=9913 GN=VPS16 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 3.9e-86
Identity = 244/828 (29.47%), Postives = 391/828 (47.22%), Query Frame = 0

Query: 8   AEWQLL-HNRYYRKPELYPMRWK-HIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 67
           A W  L  + +YRK ELY M W    +L    VA AP+GGPIA++R+  +  +    SA 
Sbjct: 6   ANWNPLGDSAFYRKYELYSMDWDLKEELRDCLVAAAPYGGPIALLRNPWR--KEKPASAR 65

Query: 68  RKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 127
             L I++ +GV LA  +W++  G ++ + W+ ++ L+CV +DG V  Y +H +    +FS
Sbjct: 66  PVLEIYSASGVPLASLLWKS--GPVVSLGWSAEEELLCVQEDGVVLVYGLHGD-FRRHFS 125

Query: 128 MGKECFEQNVVEC-VF---WGNGVVCITEANQIFCISDFKNPKSCKLSD-PGIEDLPHCM 187
           MG E  +  V++  +F   +G+GV  +T A++    ++  + K  ++ + PG+   P C 
Sbjct: 126 MGNEVLQNRVLDARIFHTEFGSGVAILTGAHRFTLSANVGDLKLRRMPEVPGLXSAPSCW 185

Query: 188 AVIEPQYTMSGNVEVLLGVGESCVI-------AVEEDGVQRLGEGVLDGPLQRMAVSLDG 247
             +           +LL VG    +       AV   G   L  GV      +MAVS   
Sbjct: 186 TTV----CQDRVAHILLAVGPDLYLLDHAACSAVTPPG---LAPGV--SSFLQMAVSFTY 245

Query: 248 KWLAAFTHDGRLLVLTSDLQKTILDREC-------------------------------- 307
           + LA FT  G + + T+ L++ + +  C                                
Sbjct: 246 RHLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMV 305

Query: 308 ------------------------------------------------------------ 367
                                                                       
Sbjct: 306 VGDAPESIQFVLDEDSYLVPELDGVRVFSRSTHEFLHEVPVASEEIFKIASMAPGALLLE 365

Query: 368 ----------EADENLRLIRP--SLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCS 427
                     +ADE LR I+    L +AV+ C++AAGHE     Q++LLRA+S+G+ F  
Sbjct: 366 AQKEYEKESQKADEYLREIQELGQLPQAVQQCIEAAGHEHWPDMQKSLLRAASFGKCFLD 425

Query: 428 NFNRERIQEMCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISE 487
            F  +    MC+ LRVLNA+R+  IGIPL+  Q+K LT  VL+ RL+    + LA++I E
Sbjct: 426 RFPPDSFVRMCQDLRVLNAIRDYHIGIPLTYSQYKQLTIQVLLDRLVLRRLYPLAIQICE 485

Query: 488 YLGMSQ----EVVIMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIG 547
           YL + +      ++ HWAC K+    ++ D  +   +  KL    G+SY+ +A  A   G
Sbjct: 486 YLRLPEVQGVSRILAHWACYKVQQK-DVSDEDVARAINQKLGDTPGVSYSDIAARAYGCG 545

Query: 548 RRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEF 607
           R +LA  L+++EPRS +QVPLLL +     AL KA ESGDTDLV+ VL H+  +    +F
Sbjct: 546 RTELAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDF 605

Query: 608 FGMIQARIQARDLFVTYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGS 667
           F  ++ +  A  L+  + +  + E LKD +       E+     + S+   +        
Sbjct: 606 FMTLRNQPMALSLYRQFCKHQELETLKDLYNQDDNHQELGSFHIRASYAAEER------- 665

Query: 668 PLHSPRIKLIEKVQSLFAETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDT 714
                R+  ++     F + K   F +KA E+  +LL++Q  LE       F+D S++DT
Sbjct: 666 --IEGRVAALQTAADAFYKAKNE-FAAKATEDQMRLLRLQRRLE-DELGGQFLDLSLHDT 725

BLAST of Cp4.1LG02g02490 vs. ExPASy Swiss-Prot
Match: Q920Q4 (Vacuolar protein sorting-associated protein 16 homolog OS=Mus musculus OX=10090 GN=Vps16 PE=1 SV=3)

HSP 1 Score: 316.2 bits (809), Expect = 9.7e-85
Identity = 247/850 (29.06%), Postives = 405/850 (47.65%), Query Frame = 0

Query: 8   AEWQLL-HNRYYRKPELYPMRWK-HIDLGRNKVACAPFGGPIAIIRDDSKIVQLYAESAL 67
           A W  L  + +YRK ELY M W    +L    VA AP+GGPIA++R+  +  +  A S  
Sbjct: 6   ANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWR--KEKAASVR 65

Query: 68  RKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELLEPNFS 127
             L I++ +G+ LA  +W++  G ++ + W+ ++ L+CV +DG V  Y +H +    +FS
Sbjct: 66  PVLEIYSASGLPLASLLWKS--GPVVALGWSAEEELLCVQEDGAVLVYGLHGD-FRRHFS 125

Query: 128 MGKECFEQNVVEC-VF---WGNGVVCITEANQIFCISDFKNPKSCKLSD-PGIEDLPHCM 187
           MG E  +  V++  +F   +G+GV  +T A +    ++  + K  ++ + PG++  P C 
Sbjct: 126 MGNEVLQNRVLDARIFHTEFGSGVAILTGAYRFTLSANVGDLKLRRMPEVPGLQSAPSCW 185

Query: 188 AVI----EPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWL 247
             +     P   ++   ++ L    +C  AV   G   L  GV      +MAVS   ++L
Sbjct: 186 TTLCHDRVPHILLAVGPDLYLLDHATC-SAVTPAG---LAPGV--SSFLQMAVSFTYRYL 245

Query: 248 AAFTHDGRLLVLTSDLQKTILDREC----------------------------------- 307
           A FT  G + + T+ L++ + +  C                                   
Sbjct: 246 ALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERAVVVAWERRLMVVGN 305

Query: 308 ------------------------------------------------------------ 367
                                                                       
Sbjct: 306 APESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLLEAQK 365

Query: 368 -------EADENLRLIRP--SLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFN 427
                  +ADE LR I+    L +AV+ C++AAGHE     Q++LLRA+S+G+ F   F 
Sbjct: 366 EYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFP 425

Query: 428 RERIQEMCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLG 487
            +    MC+ LRVLNA+R+  IGIPL+  Q+K LT  VL+ RL+    + LA++I EYL 
Sbjct: 426 PDSFVHMCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLR 485

Query: 488 MSQ----EVVIMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRK 547
           + +      ++ HWAC K  A  ++ D  +   +  KL    G+SY+ +A  A   GR +
Sbjct: 486 LPEVQGVSRILAHWACYKARA-WDMRDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTE 545

Query: 548 LAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGM 607
           LA  L+++EPRS +QVPLLL +     AL KA ESGDTDLV+ VL H+  +    +FF  
Sbjct: 546 LAIKLLEYEPRSGEQVPLLLKMKRSKLALSKAIESGDTDLVFTVLLHLKNELNRGDFFMT 605

Query: 608 IQARIQARDLFVTYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLH 667
           ++ +  A  L+  + +  + + LKD +       E+     + S+   +           
Sbjct: 606 LRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELGSFHIRASYAAEER---------I 665

Query: 668 SPRIKLIEKVQSLFAETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRT 727
             R+  ++     F + K   F +KA E+  +LL+IQ  LE       F+D S++DT+ T
Sbjct: 666 EGRVAALQTAADAFYKAKNE-FAAKATEDQMRLLRIQRRLE-DELGGRFLDLSLHDTVTT 725

Query: 728 CIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEA 739
            I+ G+++ A ++  +F++ +KR +WLK+ ALA   DW  LE FSK K+ PIGY PFVE 
Sbjct: 726 LILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEI 785

BLAST of Cp4.1LG02g02490 vs. NCBI nr
Match: XP_022940286.1 (protein VACUOLELESS1 [Cucurbita moschata] >XP_023525113.1 protein VACUOLELESS1 [Cucurbita pepo subsp. pepo] >KAG6607979.1 Protein VACUOLELESS1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037493.1 Protein VACUOLELESS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1441 bits (3731), Expect = 0.0
Identity = 747/844 (88.51%), Postives = 747/844 (88.51%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYLY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVVLIPECDGVRILSNTSTEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 780

BLAST of Cp4.1LG02g02490 vs. NCBI nr
Match: XP_022981085.1 (protein VACUOLELESS1 [Cucurbita maxima])

HSP 1 Score: 1439 bits (3726), Expect = 0.0
Identity = 745/844 (88.27%), Postives = 746/844 (88.39%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYLY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVVLIPECDGVRILSNTSTEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEAC DAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACADAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDVTLLE+LLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDVTLLEILLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 780

BLAST of Cp4.1LG02g02490 vs. NCBI nr
Match: XP_008465445.1 (PREDICTED: protein VACUOLELESS1 [Cucumis melo] >XP_008465446.1 PREDICTED: protein VACUOLELESS1 [Cucumis melo])

HSP 1 Score: 1408 bits (3644), Expect = 0.0
Identity = 723/844 (85.66%), Postives = 737/844 (87.32%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGP+AIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPVAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAG+QLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGE+CVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVVLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRA+SYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVR+PEIGIPLSIQQFKLLT PVLIARLINAHQHLLALR+SEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPD TLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK+QPLEFFGMIQAR QARDLF+TYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESW LGKNPMASKGSPLH PR KLI+K  SLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHIPRTKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH FESKAAEEHAKLLKIQH+LEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHTFESKAAEEHAKLLKIQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

BLAST of Cp4.1LG02g02490 vs. NCBI nr
Match: XP_004144632.1 (protein VACUOLELESS1 [Cucumis sativus] >XP_031739894.1 protein VACUOLELESS1 [Cucumis sativus] >KGN54934.1 hypothetical protein Csa_012058 [Cucumis sativus])

HSP 1 Score: 1405 bits (3638), Expect = 0.0
Identity = 722/844 (85.55%), Postives = 735/844 (87.09%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAG+QLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNP +CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPNACKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGE+CVIAVEEDGVQRLGEG+LDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGILDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQK ILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKIILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVFLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRA+SYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVRNPEIGIPLSIQQFKLLT PVLIARLINAHQHLLALR+SEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITAS NI D TLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASANIADATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK+QPLEFFGMIQAR QARDLF+TYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESW LGKNPMASKGSPLHSPR KLIEK  SLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHSPRTKLIEKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH+FESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHIFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

BLAST of Cp4.1LG02g02490 vs. NCBI nr
Match: XP_038898258.1 (protein VACUOLELESS1 isoform X1 [Benincasa hispida] >XP_038898259.1 protein VACUOLELESS1 isoform X1 [Benincasa hispida] >XP_038898260.1 protein VACUOLELESS1 isoform X1 [Benincasa hispida] >XP_038898261.1 protein VACUOLELESS1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1404 bits (3633), Expect = 0.0
Identity = 721/844 (85.43%), Postives = 735/844 (87.09%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAG+QLAET+WRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETIWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGE+CVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYLY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVVLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRA+SYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVR+PEIGIPLS+QQFKLLT  VLIARLINAHQHLLALR+SEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPEIGIPLSVQQFKLLTPSVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITAS N+PD TLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASSNVPDATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGD+DLVYLVLFHIWQK+QPLEFFGMIQARIQARDLF+TYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDSDLVYLVLFHIWQKRQPLEFFGMIQARIQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQL EVAFLLWKESW LGKNPMASKGSPLHSPR KLIEK  SLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLDEVAFLLWKESWELGKNPMASKGSPLHSPRTKLIEKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH+FESKAAEEHA+LLKIQH+LEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHMFESKAAEEHARLLKIQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEK E LKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKVETLKYIP 780

BLAST of Cp4.1LG02g02490 vs. ExPASy TrEMBL
Match: A0A6J1FI17 (Protein VACUOLELESS1 OS=Cucurbita moschata OX=3662 GN=LOC111445955 PE=3 SV=1)

HSP 1 Score: 1441 bits (3731), Expect = 0.0
Identity = 747/844 (88.51%), Postives = 747/844 (88.51%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYLY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVVLIPECDGVRILSNTSTEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 780

BLAST of Cp4.1LG02g02490 vs. ExPASy TrEMBL
Match: A0A6J1IYG6 (Protein VACUOLELESS1 OS=Cucurbita maxima OX=3661 GN=LOC111480344 PE=3 SV=1)

HSP 1 Score: 1439 bits (3726), Expect = 0.0
Identity = 745/844 (88.27%), Postives = 746/844 (88.39%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYLY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVVLIPECDGVRILSNTSTEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEAC DAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACADAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPDVTLLE+LLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDVTLLEILLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 780

BLAST of Cp4.1LG02g02490 vs. ExPASy TrEMBL
Match: A0A1S3CQD9 (Protein VACUOLELESS1 OS=Cucumis melo OX=3656 GN=LOC103503053 PE=3 SV=1)

HSP 1 Score: 1408 bits (3644), Expect = 0.0
Identity = 723/844 (85.66%), Postives = 737/844 (87.32%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGP+AIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPVAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAG+QLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGE+CVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVVLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRA+SYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVR+PEIGIPLSIQQFKLLT PVLIARLINAHQHLLALR+SEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPD TLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK+QPLEFFGMIQAR QARDLF+TYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESW LGKNPMASKGSPLH PR KLI+K  SLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHIPRTKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH FESKAAEEHAKLLKIQH+LEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHTFESKAAEEHAKLLKIQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

BLAST of Cp4.1LG02g02490 vs. ExPASy TrEMBL
Match: A0A0A0L1K9 (Protein VACUOLELESS1 OS=Cucumis sativus OX=3659 GN=Csa_4G608100 PE=3 SV=1)

HSP 1 Score: 1405 bits (3638), Expect = 0.0
Identity = 722/844 (85.55%), Postives = 735/844 (87.09%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAG+QLAETVWRNPGGRLIGM+WTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMAWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNP +CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPNACKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
            VIEPQYTMSGNVEVLLGVGE+CVIAVEEDGVQRLGEG+LDGPLQRMAVSLDGKWLAAFT
Sbjct: 181 VVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGILDGPLQRMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQK ILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKIILDRECESALPPQQLAWCGMDSVLLYWDDMLLMMGPDGDPVRYFY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVFLIPECDGVRILSNTSMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRA+SYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVRNPEIGIPLSIQQFKLLT PVLIARLINAHQHLLALR+SEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRNPEIGIPLSIQQFKLLTPPVLIARLINAHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITAS NI D TLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASANIADATLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK+QPLEFFGMIQAR QARDLF+TYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARTQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQL+EVAFLLWKESW LGKNPMASKGSPLHSPR KLIEK  SLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLNEVAFLLWKESWELGKNPMASKGSPLHSPRTKLIEKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEH+FESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHIFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEAC+EADEKAEA+KYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACVEADEKAEAVKYIP 780

BLAST of Cp4.1LG02g02490 vs. ExPASy TrEMBL
Match: A0A6J1C0C3 (Protein VACUOLELESS1 OS=Momordica charantia OX=3673 GN=LOC111007126 PE=3 SV=1)

HSP 1 Score: 1394 bits (3607), Expect = 0.0
Identity = 714/844 (84.60%), Postives = 733/844 (86.85%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLLHNRYYRKPELYPMRW+HIDLGRNKVACAPFGGPIAIIRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWRHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFNCAG+QLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL
Sbjct: 61  AESALRKLRIFNCAGIQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSDPGIEDLPHCM 180
           EPNFSMGKECFEQN VECVFWGNGVVCITEANQIFCISDFKNP  CKLSDPGIEDLPHCM
Sbjct: 121 EPNFSMGKECFEQNAVECVFWGNGVVCITEANQIFCISDFKNPNPCKLSDPGIEDLPHCM 180

Query: 181 AVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRLGEGVLDGPLQRMAVSLDGKWLAAFT 240
           AVIEPQYTMSGNVEVLLGVGE+CVIAVEEDGVQRLGEGVLDGPLQ+MAVSLDGKWLAAFT
Sbjct: 181 AVIEPQYTMSGNVEVLLGVGEACVIAVEEDGVQRLGEGVLDGPLQKMAVSLDGKWLAAFT 240

Query: 241 HDGRLLVLTSDLQKTILDRECE-------------------------------------- 300
           HDGRLLVLTSDLQKTILDRECE                                      
Sbjct: 241 HDGRLLVLTSDLQKTILDRECESALPPEQLAWCGMDSVLLYWDDMLLMMGPVGDPVRYFY 300

Query: 301 -----------------------------------------------------------A 360
                                                                      A
Sbjct: 301 DEPVILIPECDGVRILSNNNMEFLQRVPDSTVTIFRIGSTSPAALLYDALDHFDRRSAKA 360

Query: 361 DENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSYGQAFCSNFNRERIQEMCRLL 420
           DENLRLIR SLHEAVEACVDAAGHEFD+SRQQTLLRA+SYGQAFCSNFNRERIQEMCRLL
Sbjct: 361 DENLRLIRSSLHEAVEACVDAAGHEFDVSRQQTLLRAASYGQAFCSNFNRERIQEMCRLL 420

Query: 421 RVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLLALRISEYLGMSQEVVIMHWA 480
           RVLNAVR+P+IGIPLSIQQ+KLLT  VLIARLIN HQHLLALR+SEYLGMSQEVVIMHWA
Sbjct: 421 RVLNAVRSPDIGIPLSIQQYKLLTPSVLIARLINEHQHLLALRVSEYLGMSQEVVIMHWA 480

Query: 481 CSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADKIGRRKLAAMLVDHEPRSSKQ 540
           CSKITASLNIPD TLLE+LLDKLKLCKGISYAAVAGHADK GRRKLAAMLVDHEPRSSKQ
Sbjct: 481 CSKITASLNIPDATLLEILLDKLKLCKGISYAAVAGHADKTGRRKLAAMLVDHEPRSSKQ 540

Query: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPLEFFGMIQARIQARDLFVTYA 600
           VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQK+QPLEFFGMIQAR+QARDLF+TYA
Sbjct: 541 VPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKRQPLEFFGMIQARVQARDLFITYA 600

Query: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASKGSPLHSPRIKLIEKVQSLFA 660
           RCYKHEFLKDFFLSTGQLHEVAFLLWKESW LGKNPMASKGSPLH PRIKLI+K  SLFA
Sbjct: 601 RCYKHEFLKDFFLSTGQLHEVAFLLWKESWELGKNPMASKGSPLHGPRIKLIDKAHSLFA 660

Query: 661 ETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720
           ETKEHVFESKAAEEHAKLLK+QH+LEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT
Sbjct: 661 ETKEHVFESKAAEEHAKLLKMQHELEVSTKQAIFVDSSINDTIRTCIVLGNHRAALKVKT 720

Query: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYKPFVEACIEADEKAEALKYIP 747
           EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRP IGYKPFVEACIEADEKAEALKYIP
Sbjct: 721 EFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPAIGYKPFVEACIEADEKAEALKYIP 780

BLAST of Cp4.1LG02g02490 vs. TAIR 10
Match: AT2G38020.1 (vacuoleless1 (VCL1) )

HSP 1 Score: 1095.5 bits (2832), Expect = 0.0e+00
Identity = 572/865 (66.13%), Postives = 653/865 (75.49%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLL++RYYRKPE+Y M+WKH+DL RNKVACA FGGPIA+IRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLYDRYYRKPEIYQMKWKHVDLSRNKVACASFGGPIAVIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFN AG+ L+ETVW++PGGRLIGMSW+DDQTL+C+VQDGT+YRYNIHAEL+
Sbjct: 61  AESALRKLRIFNSAGILLSETVWKHPGGRLIGMSWSDDQTLICIVQDGTIYRYNIHAELI 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSD-PGI--EDL- 180
           EPN SMG+ECFEQNVVECVFWGNGVVC+TE  Q+ CI DFK  K  KL D PG+  +DL 
Sbjct: 121 EPNMSMGQECFEQNVVECVFWGNGVVCLTEGGQLICIFDFKTMKPSKLPDVPGLAEDDLL 180

Query: 181 -PHCMAVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRL---------------GEGVL 240
            P C+ V EP+YTMSG  EVL+ VG+  +  VEED VQ +                 G L
Sbjct: 181 QPICLTVREPKYTMSGIAEVLVAVGDD-IFVVEEDMVQTIRFDEPSVDDSEMQNDDSGNL 240

Query: 241 DGPLQRMAVSLDGKWLAAFTHDGRLLVLTSDLQKTILDRECE------------------ 300
            G +Q+M VS +GK+L  FTHDGR++V+  + ++  +D  CE                  
Sbjct: 241 IGVVQKMIVSPNGKFLTLFTHDGRIVVVDMETKQIAIDYSCESALPPQQMAWCGMDSVLL 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 YWDEDLMMVGPVGDPVHYFYDEPIILIPECDGVRILSNTNLEFLQRVPDSTESIFKIGST 360

Query: 361 -------------------ADENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSY 420
                              ADENLRLIR SL EAVE+C+DAAGHEFD++RQ+ LLRA+SY
Sbjct: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLSEAVESCIDAAGHEFDVTRQRALLRAASY 420

Query: 421 GQAFCSNFNRERIQEMCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLL 480
           GQAFCSNF RER+QE CR LRVLNAVR+P IGIPLSIQQ+KLLT  VLI+RLINA+ HLL
Sbjct: 421 GQAFCSNFQRERVQETCRTLRVLNAVRDPAIGIPLSIQQYKLLTPVVLISRLINANCHLL 480

Query: 481 ALRISEYLGMSQEVVIMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADK 540
           ALRISEYL M++EVVIMHWAC+KITAS + PD  LLE+LLDKL+LCKGISYAAVA HAD 
Sbjct: 481 ALRISEYLDMNKEVVIMHWACAKITASPSTPDSHLLEILLDKLQLCKGISYAAVATHADN 540

Query: 541 IGRRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPL 600
            GRRKLAAMLV+HEPRS+KQVPLLLSIGEEDTAL+KATESGDTDLVYLV+FHIWQK+ PL
Sbjct: 541 CGRRKLAAMLVEHEPRSTKQVPLLLSIGEEDTALVKATESGDTDLVYLVIFHIWQKRPPL 600

Query: 601 EFFGMIQARIQARDLFVTYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASK 660
           EFF MIQ R+ ARDLFV YARC+KHEFLKDFFLSTGQ+HEVAFLLWKESW +GKNPMASK
Sbjct: 601 EFFAMIQGRVLARDLFVAYARCHKHEFLKDFFLSTGQIHEVAFLLWKESWDMGKNPMASK 660

Query: 661 GSPLHSPRIKLIEKVQSLFAETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSIN 720
           GSPLH PRIKLIEK ++LF++TKEH FESKAAEEHAKLLKIQH+LE STKQAIFVDSSIN
Sbjct: 661 GSPLHGPRIKLIEKARNLFSQTKEHTFESKAAEEHAKLLKIQHELEASTKQAIFVDSSIN 720

Query: 721 DTIRTCIVLGNHRAALKVKTEFKVSEKRWYWLKVFALATTRDWVALETFSKEKRPPIGYK 748
           DTIRTCIVLGN+RAA+KVKTEFKVS+KRWYWLK FALAT +DW ALE FSKEKRPP+G++
Sbjct: 721 DTIRTCIVLGNNRAAIKVKTEFKVSDKRWYWLKAFALATIKDWAALEKFSKEKRPPMGFR 780

BLAST of Cp4.1LG02g02490 vs. TAIR 10
Match: AT2G38020.2 (vacuoleless1 (VCL1) )

HSP 1 Score: 1048.9 bits (2711), Expect = 1.9e-306
Identity = 538/810 (66.42%), Postives = 615/810 (75.93%), Query Frame = 0

Query: 1   MANVSVAAEWQLLHNRYYRKPELYPMRWKHIDLGRNKVACAPFGGPIAIIRDDSKIVQLY 60
           MANVSVAAEWQLL++RYYRKPE+Y M+WKH+DL RNKVACA FGGPIA+IRDDSKIVQLY
Sbjct: 1   MANVSVAAEWQLLYDRYYRKPEIYQMKWKHVDLSRNKVACASFGGPIAVIRDDSKIVQLY 60

Query: 61  AESALRKLRIFNCAGVQLAETVWRNPGGRLIGMSWTDDQTLVCVVQDGTVYRYNIHAELL 120
           AESALRKLRIFN AG+ L+ETVW++PGGRLIGMSW+DDQTL+C+VQDGT+YRYNIHAEL+
Sbjct: 61  AESALRKLRIFNSAGILLSETVWKHPGGRLIGMSWSDDQTLICIVQDGTIYRYNIHAELI 120

Query: 121 EPNFSMGKECFEQNVVECVFWGNGVVCITEANQIFCISDFKNPKSCKLSD-PGI--EDL- 180
           EPN SMG+ECFEQNVVECVFWGNGVVC+TE  Q+ CI DFK  K  KL D PG+  +DL 
Sbjct: 121 EPNMSMGQECFEQNVVECVFWGNGVVCLTEGGQLICIFDFKTMKPSKLPDVPGLAEDDLL 180

Query: 181 -PHCMAVIEPQYTMSGNVEVLLGVGESCVIAVEEDGVQRL---------------GEGVL 240
            P C+ V EP+YTMSG  EVL+ VG+  +  VEED VQ +                 G L
Sbjct: 181 QPICLTVREPKYTMSGIAEVLVAVGDD-IFVVEEDMVQTIRFDEPSVDDSEMQNDDSGNL 240

Query: 241 DGPLQRMAVSLDGKWLAAFTHDGRLLVLTSDLQKTILDRECE------------------ 300
            G +Q+M VS +GK+L  FTHDGR++V+  + ++  +D  CE                  
Sbjct: 241 IGVVQKMIVSPNGKFLTLFTHDGRIVVVDMETKQIAIDYSCESALPPQQMAWCGMDSVLL 300

Query: 301 ------------------------------------------------------------ 360
                                                                       
Sbjct: 301 YWDEDLMMVGPVGDPVHYFYDEPIILIPECDGVRILSNTNLEFLQRVPDSTESIFKIGST 360

Query: 361 -------------------ADENLRLIRPSLHEAVEACVDAAGHEFDISRQQTLLRASSY 420
                              ADENLRLIR SL EAVE+C+DAAGHEFD++RQ+ LLRA+SY
Sbjct: 361 SPAALLYDALDHFDRRSAKADENLRLIRSSLSEAVESCIDAAGHEFDVTRQRALLRAASY 420

Query: 421 GQAFCSNFNRERIQEMCRLLRVLNAVRNPEIGIPLSIQQFKLLTAPVLIARLINAHQHLL 480
           GQAFCSNF RER+QE CR LRVLNAVR+P IGIPLSIQQ+KLLT  VLI+RLINA+ HLL
Sbjct: 421 GQAFCSNFQRERVQETCRTLRVLNAVRDPAIGIPLSIQQYKLLTPVVLISRLINANCHLL 480

Query: 481 ALRISEYLGMSQEVVIMHWACSKITASLNIPDVTLLEVLLDKLKLCKGISYAAVAGHADK 540
           ALRISEYL M++EVVIMHWAC+KITAS + PD  LLE+LLDKL+LCKGISYAAVA HAD 
Sbjct: 481 ALRISEYLDMNKEVVIMHWACAKITASPSTPDSHLLEILLDKLQLCKGISYAAVATHADN 540

Query: 541 IGRRKLAAMLVDHEPRSSKQVPLLLSIGEEDTALIKATESGDTDLVYLVLFHIWQKKQPL 600
            GRRKLAAMLV+HEPRS+KQVPLLLSIGEEDTAL+KATESGDTDLVYLV+FHIWQK+ PL
Sbjct: 541 CGRRKLAAMLVEHEPRSTKQVPLLLSIGEEDTALVKATESGDTDLVYLVIFHIWQKRPPL 600

Query: 601 EFFGMIQARIQARDLFVTYARCYKHEFLKDFFLSTGQLHEVAFLLWKESWVLGKNPMASK 660
           EFF MIQ R+ ARDLFV YARC+KHEFLKDFFLSTGQ+HEVAFLLWKESW +GKNPMASK
Sbjct: 601 EFFAMIQGRVLARDLFVAYARCHKHEFLKDFFLSTGQIHEVAFLLWKESWDMGKNPMASK 660

Query: 661 GSPLHSPRIKLIEKVQSLFAETKEHVFESKAAEEHAKLLKIQHDLEVSTKQAIFVDSSIN 694
           GSPLH PRIKLIEK ++LF++TKEH FESKAAEEHAKLLKIQH+LE STKQAIFVDSSIN
Sbjct: 661 GSPLHGPRIKLIEKARNLFSQTKEHTFESKAAEEHAKLLKIQHELEASTKQAIFVDSSIN 720

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93VQ00.0e+0066.13Protein VACUOLELESS1 OS=Arabidopsis thaliana OX=3702 GN=VCL1 PE=1 SV=1[more]
Q55C588.1e-11632.42Vacuolar protein sorting-associated protein 16 homolog OS=Dictyostelium discoide... [more]
Q9H2697.9e-8729.59Vacuolar protein sorting-associated protein 16 homolog OS=Homo sapiens OX=9606 G... [more]
Q5E9L73.9e-8629.47Vacuolar protein sorting-associated protein 16 homolog OS=Bos taurus OX=9913 GN=... [more]
Q920Q49.7e-8529.06Vacuolar protein sorting-associated protein 16 homolog OS=Mus musculus OX=10090 ... [more]
Match NameE-valueIdentityDescription
XP_022940286.10.088.51protein VACUOLELESS1 [Cucurbita moschata] >XP_023525113.1 protein VACUOLELESS1 [... [more]
XP_022981085.10.088.27protein VACUOLELESS1 [Cucurbita maxima][more]
XP_008465445.10.085.66PREDICTED: protein VACUOLELESS1 [Cucumis melo] >XP_008465446.1 PREDICTED: protei... [more]
XP_004144632.10.085.55protein VACUOLELESS1 [Cucumis sativus] >XP_031739894.1 protein VACUOLELESS1 [Cuc... [more]
XP_038898258.10.085.43protein VACUOLELESS1 isoform X1 [Benincasa hispida] >XP_038898259.1 protein VACU... [more]
Match NameE-valueIdentityDescription
A0A6J1FI170.088.51Protein VACUOLELESS1 OS=Cucurbita moschata OX=3662 GN=LOC111445955 PE=3 SV=1[more]
A0A6J1IYG60.088.27Protein VACUOLELESS1 OS=Cucurbita maxima OX=3661 GN=LOC111480344 PE=3 SV=1[more]
A0A1S3CQD90.085.66Protein VACUOLELESS1 OS=Cucumis melo OX=3656 GN=LOC103503053 PE=3 SV=1[more]
A0A0A0L1K90.085.55Protein VACUOLELESS1 OS=Cucumis sativus OX=3659 GN=Csa_4G608100 PE=3 SV=1[more]
A0A6J1C0C30.084.60Protein VACUOLELESS1 OS=Momordica charantia OX=3673 GN=LOC111007126 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38020.10.0e+0066.13vacuoleless1 (VCL1) [more]
AT2G38020.21.9e-30666.42vacuoleless1 (VCL1) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038132Vps16, C-terminal domain superfamilyGENE3D1.10.150.780coord: 548..642
e-value: 1.8E-28
score: 100.8
IPR016534Vacuolar protein sorting-associated protein 16PIRSFPIRSF007949Vps16coord: 1..264
e-value: 8.5E-60
score: 200.8
coord: 258..745
e-value: 2.3E-190
score: 632.7
IPR016534Vacuolar protein sorting-associated protein 16PANTHERPTHR12811VACUOLAR PROTEIN SORTING VPS16coord: 1..261
coord: 262..740
IPR006926Vps16, N-terminalPFAMPF04841Vps16_Ncoord: 262..314
e-value: 5.5E-11
score: 41.9
coord: 8..262
e-value: 3.1E-31
score: 108.6
IPR006925Vps16, C-terminalPFAMPF04840Vps16_Ccoord: 412..726
e-value: 7.5E-66
score: 222.4
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 67..250

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG02g02490.1Cp4.1LG02g02490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016197 endosomal transport
biological_process GO:0006886 intracellular protein transport
biological_process GO:0045992 negative regulation of embryonic development
biological_process GO:0042144 vacuole fusion, non-autophagic
biological_process GO:0051469 vesicle fusion with vacuole
biological_process GO:0007033 vacuole organization
cellular_component GO:0033263 CORVET complex
cellular_component GO:0030897 HOPS complex
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003779 actin binding
molecular_function GO:0005515 protein binding