Cp4.1LG02g02240 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG02g02240
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	glucan endo-1,3-beta-glucosidase-like
Location	Cp4.1LG02: 3982467 .. 3985758 (-)
RNA-Seq Expression	Cp4.1LG02g02240
Synteny	Cp4.1LG02g02240

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAGCCGTTGCAATCCCCACAAATCCACCTTCCGCCTATAAAATCCACTTCCGTTCTCCACTTTTTCCAATCATCTCACACTCCAAATCGCCTTTTTTCTCATCGCTTTTCTCCAGATCTCTGCCTCTTCCTTCAATGGCGAAAGCAGTCGCAGGAGCAGTCACTCTCTCCTTTCTGCTTCTGTTCCTCTCAGGTTCGTTCTCCTGATCTCTTCGAATTCCGCGTCGTTTTTTGCGTACCTAACTTTCCTTCTTTCTTCTTTCTTCTTCCAATTCTTTTTTCAGGAGATCTCTTGGCGGTGAATGGATATCAGGTGAATCGATTGTTATCGAAATGCAGAGTAACGGATTAGAATAATCCGATGAAATCTGAGATGATTTTTGTTTTTGTTTTTGTTTTGCAGAAGACGTGGTGCGTTGCGAGGCCTTCGTCGGATCACGCGACGCTGTTAGCGAATATCGATTATGCTTGTTCACAAGTGGATTGTAGGATTATGCAGAAAGGATGCCCCTGCTTTTATCCTGATAATCTGATTAACCATGCCTCCATCGCTATGAATCTGTATTACAATTCGAAAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGATCTGGTCTCATCGTCATCACTGATCCAAGTTAGTTCATGATTTAAACAGATTTTGCTGTTGTGTTTGTCCTTTTGTTCTTGATTTTGATTTTGTGGTTCGATTTTCGCTGCTGCAGGTTATGGGAACTGCCTTTACCCTTGAATTGAAGTGAAGCATAGAAGTGTAGGAGAATCACACAGCTCAAAAGTAAAATTTATTGTTAGTTTATTGATTATAAAGTTTGATTATATATACTTAGGTGGGAAGAGATAGATACTGAATTAATGCTATTTATCCTCTATTCAATTCAATATCAAAATTAAATCAAATTAGGGTTTGAATCTAAACTTCTACGAATTAGGCACTCCTCAATGTTTATGTATATATTGTTAGACGAACACAACTCTCTACAATATTTTGATATTGTCCACTATGAGTATAAGCTCTCATAGTTTTGCTTTCGACTTCCTCATGAGTTCTCATACCAATGGATAGAATATTCTTTATTTATAAACCCATAATTATTCTCTAAATTAGCCTACTTGTGACTCTCATCATCCAACACCTCCCCGAACAAAGAGTCTTAGTCATTTTTTACTGCCTTCGAGGAGGCTTGACTCTTTTTTTCTTTTGGAGTCTTTTGTTCGTCATTTGAGGATTTACCAATCTATTGGCACGACTAAGTTTAGGACACACATTTGAGGATTTACCAATCTATTGGCACGACGCTTAGGGCATAGTTCTGATACCATGCTAGATGAACACAACTCTCAACAAATAGTATGATATTGTCTACTTTGGACAGAAACTCTGATGGTTTTGGTTTGAGCTTACCCAGAAGGGCCTCATACTAATGGACTTTCATCATTAACATATATATGTGAAAGGAGGTTTATCACGAAATACTTATCTTTCAATAGAATTGATTGTTTACGTAAAATACAAAAAAAATGAACAATAATAATTTAATCCATAAGATTAAAAAAACGGAGAGATATCTTTCACCGATTAAAAAATTAATTTAAATATACGTAATAATAAGTTTGATGACTTATCTTTTTGAGTAAACATTAAAATAATGTTGATGAAAAATGTTTTTTTTTTTTCATAAATTTTTTTTAAATATTTTTTTATCCAAAACTTTTAGCTAAGAGATTTTTAAAAGATAGTTTCTTAATTATTATTATTATTATTATTATTATTTTGAAGATTAAAAGAATTATTGATTAAGTTTAGAATATTTCTATAATTTAGTAAAAGAAAAGAAACCTTATAAGGGTAAAAGCGTAATTTTAACCGAAGTATTCACTTATAATTAGAAGCATCGGGGACTCGAGCAAAAGAAGAGAAACCTTAAGCGAAGGGTAAAAGCGTAATTTTGCTGAAGTATTCGCCTATTTAATCTGGAGATGGTCTAGGGTTTGAGGCATTGCCACTGAGAGAACACAGCATCTGTAAAAAAGATGGTGCTGTTGTTCCGTTGCGGGCATAGAACAAATCGATTCCTTTCTCTAATTTTCGATCTGGAAGTTTGATTCTCGTCAAGCAGTTTGCAGTTTCGAAGATGAAAGGTTTTTACATTCGTTGTTTAATCTTTTTCTAATCGTTTTCTTCAAGGCCGATCCAATGGGTTCTCGGACGTTAAATCGAATACTCGAGAGCTCAATTTCACCCGGCCGAGATTTTCTTTGCGGCAGCCTTAAGGTTTTGCATGTTAATCTCATTCATGCTCACATTCAGGAATCTGCCCTAAATTTTCTCTCCAATCTGTGCCTAGATTTTTGTTTTGCTTCGATTAGGTTTGAATGTTGAAGTTTGTGATTGAATCAATCTGTTCGTAGGAGATAGGTAGAGATCTTCGTCGACAAACGACGACATTCCCTAGGCCGCTAGGGTTTACCTTTTGCGTAGAAGTTGGATCTCATTGTTTGAACGAAATACAGAGCAATTAAGGTTAGTTGAAGTGAATCGATACCAATTATTTGTAACTGTTGGATATCCGTTTGGTACCATCACTATGATTCGATTCTGTAGATTGAGAAAATGAGTATATACTAATTTCAGGTTAAGATTATGGATTGAACTTGGTCGATCTGTCTTGCATTTTTGTTCAATGTATGAGCTTGATCTGGGTTTAATGCTTTTCAGAATTTGAGCTTTGTTTTTTAAACTTTTTGTTCGTTTAATTCTGTATTATGTTTTTGGTATGTTCTTAATGTATTATATTTCATGTTATGAACACACTTTATTTTTGCTAAATCAAAGTATATTATAATGTCTGAATGATTGAGTTATTTATATTAGACATAACTTATACAACACAAAAAGGGAATGATATATGAACTTTGAAACTATTAGAGCTTACGGAGGATGAGATGAGCTCCATGTTGTGGTTGAACAGTGACATTTGAAGCAGGAGAGTGAGTATAAGATGGGGAAAGTTGGAAGGAGAAGCTCTGCAAAATCATTGATAGTGCTATTTTTGCTTCAATCATGGCAAAGTTTTGACCAACACAAATTCGAGGACCCCATCCAAATGGTAGGAAGCAAACTTTATTGTTTGTTGCTTTGGAAATTCCTTCTGAAAACCTCTCTGGCTTGAACTCATCTGCATCTTCACCCCATATTTGGTGATCGTGGTGGATATGAATGATTGGTATGCCTAATGACACCCCACCCGGCAATATCAAGTTTCCAAGCTT

mRNA sequence

GTAGCCGTTGCAATCCCCACAAATCCACCTTCCGCCTATAAAATCCACTTCCGTTCTCCACTTTTTCCAATCATCTCACACTCCAAATCGCCTTTTTTCTCATCGCTTTTCTCCAGATCTCTGCCTCTTCCTTCAATGGCGAAAGCAGTCGCAGGAGCAGTCACTCTCTCCTTTCTGCTTCTGTTCCTCTCAGGAGATCTCTTGGCGGTGAATGGATATCAGAAGACGTGGTGCGTTGCGAGGCCTTCGTCGGATCACGCGACGCTGTTAGCGAATATCGATTATGCTTGTTCACAAGTGGATTGTAGGATTATGCAGAAAGGATGCCCCTGCTTTTATCCTGATAATCTGATTAACCATGCCTCCATCGCTATGAATCTGTATTACAATTCGAAAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGATCTGGTCTCATCGTCATCACTGATCCAAGTTATGGGAACTGCCTTTACCCTTGAATTGAAGTGAAGCATAGAAGTGTAGGAGAATCACACAGCTCAAAAGTAAAATTTATTGTTAGTTTATTGATTATAAAGTTTGATTATATATACTTAGGTGGGAAGAGATAGATACTGAATTAATGCTATTTATCCTCTATTCAATTCAATATCAAAATTAAATCAAATTAGGGTTTGAATCTAAACTTCTACGAATTAGGCACTCCTCAATGTTTATGTATATATTGTTAGACGAACACAACTCTCTACAATATTTTGATATTGTCCACTATGAGTATAAGCTCTCATAGTTTTGCTTTCGACTTCCTCATGAGTTCTCATACCAATGGATAGAATATTCTTTATTTATAAACCCATAATTATTCTCTAAATTAGCCTACTTGTGACTCTCATCATCCAACACCTCCCCGAACAAAGAGTCTTAGTCATTTTTTACTGCCTTCGAGGAGGCTTGACTCTTTTTTTCTTTTGGAGTCTTTTGTTCGTCATTTGAGGATTTACCAATCTATTGGCACGACTAAGTTTAGGACACACATTTGAGGATTTACCAATCTATTGGCACGACGCTTAGGGCATAGTTCTGATACCATGCTAGATGAACACAACTCTCAACAAATAGTATGATATTGTCTACTTTGGACAGAAACTCTGATGGTTTTGGTTTGAGCTTACCCAGAAGGGCCTCATACTAATGGACTTTCATCATTAACATATATATGTGAAAGGAGGTTTATCACGAAATACTTATCTTTCAATAGAATTGATTGTTTACGTAAAATACAAAAAAAATGAACAATAATAATTTAATCCATAAGATTAAAAAAACGGAGAGATATCTTTCACCGATTAAAAAATTAATTTAAATATACGTAATAATAAGTTTGATGACTTATCTTTTTGAGTAAACATTAAAATAATGTTGATGAAAAATGTTTTTTTTTTTTCATAAATTTTTTTTAAATATTTTTTTATCCAAAACTTTTAGCTAAGAGATTTTTAAAAGATAGTTTCTTAATTATTATTATTATTATTATTATTATTTTGAAGATTAAAAGAATTATTGATTAAGTTTAGAATATTTCTATAATTTAGTAAAAGAAAAGAAACCTTATAAGGGTAAAAGCGTAATTTTAACCGAAGTATTCACTTATAATTAGAAGCATCGGGGACTCGAGCAAAAGAAGAGAAACCTTAAGCGAAGGGTAAAAGCGTAATTTTGCTGAAGTATTCGCCTATTTAATCTGGAGATGGTCTAGGGTTTGAGGCATTGCCACTGAGAGAACACAGCATCTGTAAAAAAGATGGTGCTGTTGTTCCGTTGCGGGCATAGAACAAATCGATTCCTTTCTCTAATTTTCGATCTGGAAGTTTGATTCTCGTCAAGCAGTTTGCAGTTTCGAAGATGAAAGGTTTTTACATTCGTTGTTTAATCTTTTTCTAATCGTTTTCTTCAAGGCCGATCCAATGGGTTCTCGGACGTTAAATCGAATACTCGAGAGCTCAATTTCACCCGGCCGAGATTTTCTTTGCGGCAGCCTTAAGGTTTTGCATGTTAATCTCATTCATGCTCACATTCAGGAATCTGCCCTAAATTTTCTCTCCAATCTGTGCCTAGATTTTTGTTTTGCTTCGATTAGGTTTGAATGTTGAAGTTTGTGATTGAATCAATCTGTTCGTAGGAGATAGGTAGAGATCTTCGTCGACAAACGACGACATTCCCTAGGCCGCTAGGGTTTACCTTTTGCGTAGAAGTTGGATCTCATTGTTTGAACGAAATACAGAGCAATTAAGGTTAAGATTATGGATTGAACTTGGTCGATCTGTCTTGCATTTTTGTTCAATGTATGAGCTTGATCTGGGTTTAATGCTTTTCAGAATTTGAGCTTTGTTTTTTAAACTTTTTGTTCGTTTAATTCTGTATTATGTTTTTGGTATGTTCTTAATGTATTATATTTCATGTTATGAACACACTTTATTTTTGCTAAATCAAAGTATATTATAATGTCTGAATGATTGAGTTATTTATATTAGACATAACTTATACAACACAAAAAGGGAATGATATATGAACTTTGAAACTATTAGAGCTTACGGAGGATGAGATGAGCTCCATGTTGTGGTTGAACAGTGACATTTGAAGCAGGAGAGTGAGTATAAGATGGGGAAAGTTGGAAGGAGAAGCTCTGCAAAATCATTGATAGTGCTATTTTTGCTTCAATCATGGCAAAGTTTTGACCAACACAAATTCGAGGACCCCATCCAAATGGTAGGAAGCAAACTTTATTGTTTGTTGCTTTGGAAATTCCTTCTGAAAACCTCTCTGGCTTGAACTCATCTGCATCTTCACCCCATATTTGGTGATCGTGGTGGATATGAATGATTGGTATGCCTAATGACACCCCACCCGGCAATATCAAGTTTCCAAGCTT

Coding sequence (CDS)

GTAGCCGTTGCAATCCCCACAAATCCACCTTCCGCCTATAAAATCCACTTCCGTTCTCCACTTTTTCCAATCATCTCACACTCCAAATCGCCTTTTTTCTCATCGCTTTTCTCCAGATCTCTGCCTCTTCCTTCAATGGCGAAAGCAGTCGCAGGAGCAGTCACTCTCTCCTTTCTGCTTCTGTTCCTCTCAGGAGATCTCTTGGCGGTGAATGGATATCAGAAGACGTGGTGCGTTGCGAGGCCTTCGTCGGATCACGCGACGCTGTTAGCGAATATCGATTATGCTTGTTCACAAGTGGATTGTAGGATTATGCAGAAAGGATGCCCCTGCTTTTATCCTGATAATCTGATTAACCATGCCTCCATCGCTATGAATCTGTATTACAATTCGAAAGGGAAGAATCAGTGGAACTGTGATTTCAGAGGATCTGGTCTCATCGTCATCACTGATCCAAGTTATGGGAACTGCCTTTACCCTTGA

Protein sequence

VAVAIPTNPPSAYKIHFRSPLFPIISHSKSPFFSSLFSRSLPLPSMAKAVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP

Homology

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy Swiss-Prot
Match: P52409 (Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 3.4e-19
Identity = 39/83 (46.99%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 77  WCVARPSSDHATLLANIDYACSQVDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQ 136
           WCVA+  ++   L  NI+YAC  VDC+ +Q G  CF P++L  HAS  MN YY + G   
Sbjct: 377 WCVAKDGANGTDLQNNINYACGFVDCKPIQSGGACFSPNSLQAHASYVMNAYYQANGHTD 436

Query: 137 WNCDFRGSGLIVITDPSYGNCLY 160
             CDF+G+G++  +DPSYG C Y
Sbjct: 437 LACDFKGTGIVTSSDPSYGGCKY 459

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy Swiss-Prot
Match: Q94G86 (Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 4.4e-19
Identity = 40/86 (46.51%), Postives = 53/86 (61.63%), Query Frame = 0


Query: 76  TWCVARPSSDHATLLANIDYACSQ-VDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGK 135
           +WCV +P      L  NI+YAC Q +DC  +Q G  CF P+ +  HA+  MNLYY S G+
Sbjct: 371 SWCVPKPGVSDDQLTGNINYACGQGIDCGPIQPGGACFEPNTVKAHAAYVMNLYYQSAGR 430

Query: 136 NQWNCDFRGSGLIVITDPSYGNCLYP 161
           N WNCDF  +  +  T+PSYG C +P
Sbjct: 431 NSWNCDFSQTATLTNTNPSYGACNFP 456

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy Swiss-Prot
Match: Q84V39 (Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 2.9e-18
Identity = 41/87 (47.13%), Postives = 56/87 (64.37%), Query Frame = 0


Query: 75  KTWCVARPSSDHATLLANIDYACSQ--VDCRIMQKGCPCFYPDNLINHASIAMNLYYNSK 134
           K WCV +  +  A L +NIDY CSQ  +DC  +Q    CF P+ +  HAS AMN +Y SK
Sbjct: 35  KKWCVPKAEATDAQLQSNIDYVCSQSGMDCGPIQANGACFNPNTVRAHASYAMNSWYQSK 94

Query: 135 GKNQWNCDFRGSGLIVITDPSYGNCLY 160
           G+N ++CDF G+G I  +DPS G+C +
Sbjct: 95  GRNDFDCDFSGTGAITSSDPSNGSCSF 121

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 92.0 bits (227), Expect = 6.4e-18
Identity = 51/149 (34.23%), Postives = 82/149 (55.03%), Query Frame = 0


Query: 19  SPLFPIISHSKSPFFSSLFSRSLPLPSMAKAVAG-----AVTLSFLLLFLSGDLLAVNGY 78
           +PL P    + S +   LF+  L  P +++A  G     +  +  L +  SG  LA +  
Sbjct: 320 TPLHP--EMTSSVYIYELFNEDLRAPPVSEASWGLFYGNSTPVYLLHVSGSGTFLANDTT 379

Query: 79  QKTWCVARPSSDHATLLANIDYAC--SQVDCRIMQKGCPCFYPDNLINHASIAMNLYYNS 138
            +T+C+A    D  TL A +D+AC   + +C  +Q G  C+ P+N+  HAS A N YY  
Sbjct: 380 NQTYCIAMDGVDAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQK 439

Query: 139 KGKNQWNCDFRGSGLIVITDPSYGNCLYP 161
           +G+   +CDF+G  +I  TDPS+G+C++P
Sbjct: 440 EGRASGSCDFKGVAMITTTDPSHGSCIFP 466

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.9e-17
Identity = 39/90 (43.33%), Postives = 51/90 (56.67%), Query Frame = 0


Query: 72  GYQKTWCVARPSSDHATLLANIDYAC--SQVDCRIMQKGCPCFYPDNLINHASIAMNLYY 131
           G  K WC+A   +    L   +D+AC    VDC  +Q   PCF PD +++HAS A N YY
Sbjct: 386 GGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTYY 445

Query: 132 NSKGKNQWNCDFRGSGLIVITDPSYGNCLY 160
              G +  +C F G+ + V  DPSYGNCLY
Sbjct: 446 QQSGASSIDCSFNGASVEVDKDPSYGNCLY 475

[Close]

BLAST of Cp4.1LG02g02240 vs. NCBI nr
Match: XP_023525198.1 (major pollen allergen Ole e 10-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 249 bits (635), Expect = 1.14e-82
Identity = 115/115 (100.00%), Postives = 115/115 (100.00%), Query Frame = 0


Query: 46  MAKAVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIM 105
           MAKAVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIM
Sbjct: 1   MAKAVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIM 60

Query: 106 QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 115

[Close]

BLAST of Cp4.1LG02g02240 vs. NCBI nr
Match: XP_023525199.1 (major pollen allergen Ole e 10-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 242 bits (617), Expect = 6.13e-80
Identity = 114/115 (99.13%), Postives = 114/115 (99.13%), Query Frame = 0


Query: 46  MAKAVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIM 105
           MAKAVAGAVTLSFLLLFLSGDLLAVNGYQ TWCVARPSSDHATLLANIDYACSQVDCRIM
Sbjct: 1   MAKAVAGAVTLSFLLLFLSGDLLAVNGYQ-TWCVARPSSDHATLLANIDYACSQVDCRIM 60

Query: 106 QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 61  QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 114

[Close]

BLAST of Cp4.1LG02g02240 vs. NCBI nr
Match: XP_022982185.1 (major pollen allergen Ole e 10-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 237 bits (604), Expect = 5.69e-78
Identity = 109/112 (97.32%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 49  AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 108
           A A AVTLSFLLLFLSGDLLAVNGYQKTWCV RPSSDHATLLANIDYACSQVDCRIMQKG
Sbjct: 2   AKAAAVTLSFLLLFLSGDLLAVNGYQKTWCVPRPSSDHATLLANIDYACSQVDCRIMQKG 61

Query: 109 CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Cp4.1LG02g02240 vs. NCBI nr
Match: XP_022941529.1 (glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata] >KAG7037466.1 Glucan endo-1,3-beta-D-glucosidase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 235 bits (599), Expect = 3.29e-77
Identity = 108/112 (96.43%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 49  AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 108
           A A AV LSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANI+YACSQVDCRIMQKG
Sbjct: 2   AKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQKG 61

Query: 109 CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Cp4.1LG02g02240 vs. NCBI nr
Match: KAG6607951.1 (Glucan endo-1,3-beta-D-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 234 bits (597), Expect = 6.64e-77
Identity = 107/112 (95.54%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 49  AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 108
           A A AV +SFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANI+YACSQVDCRIMQKG
Sbjct: 2   AKAAAVAISFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQKG 61

Query: 109 CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy TrEMBL
Match: A0A6J1J1Y2 (major pollen allergen Ole e 10-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481088 PE=4 SV=1)

HSP 1 Score: 237 bits (604), Expect = 2.75e-78
Identity = 109/112 (97.32%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 49  AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 108
           A A AVTLSFLLLFLSGDLLAVNGYQKTWCV RPSSDHATLLANIDYACSQVDCRIMQKG
Sbjct: 2   AKAAAVTLSFLLLFLSGDLLAVNGYQKTWCVPRPSSDHATLLANIDYACSQVDCRIMQKG 61

Query: 109 CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy TrEMBL
Match: A0A6J1FNL6 (glucan endo-1,3-beta-glucosidase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446803 PE=4 SV=1)

HSP 1 Score: 235 bits (599), Expect = 1.59e-77
Identity = 108/112 (96.43%), Postives = 109/112 (97.32%), Query Frame = 0


Query: 49  AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 108
           A A AV LSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANI+YACSQVDCRIMQKG
Sbjct: 2   AKAAAVALSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANINYACSQVDCRIMQKG 61

Query: 109 CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 113

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy TrEMBL
Match: A0A6J1IYM5 (major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481088 PE=4 SV=1)

HSP 1 Score: 230 bits (586), Expect = 1.48e-75
Identity = 108/112 (96.43%), Postives = 108/112 (96.43%), Query Frame = 0


Query: 49  AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 108
           A A AVTLSFLLLFLSGDLLAVNGYQ TWCV RPSSDHATLLANIDYACSQVDCRIMQKG
Sbjct: 2   AKAAAVTLSFLLLFLSGDLLAVNGYQ-TWCVPRPSSDHATLLANIDYACSQVDCRIMQKG 61

Query: 109 CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 112

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy TrEMBL
Match: A0A6J1FTX7 (major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446803 PE=4 SV=1)

HSP 1 Score: 228 bits (581), Expect = 8.55e-75
Identity = 107/112 (95.54%), Postives = 108/112 (96.43%), Query Frame = 0


Query: 49  AVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIMQKG 108
           A A AV LSFLLLFLSGDLLAVNGYQ TWCVARPSSDHATLLANI+YACSQVDCRIMQKG
Sbjct: 2   AKAAAVALSFLLLFLSGDLLAVNGYQ-TWCVARPSSDHATLLANINYACSQVDCRIMQKG 61

Query: 109 CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP
Sbjct: 62  CPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 112

[Close]

BLAST of Cp4.1LG02g02240 vs. ExPASy TrEMBL
Match: A0A6J1CBF4 (glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC111009159 PE=4 SV=1)

HSP 1 Score: 201 bits (510), Expect = 6.01e-64
Identity = 95/115 (82.61%), Postives = 102/115 (88.70%), Query Frame = 0


Query: 50  VAGAVTLSFLLLFLS----GDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIM 109
           +A A  L F LLFLS    GDLLAVNG +KTWCVARPSSD ATLLANI+YACSQVDCRIM
Sbjct: 1   MAAAAALCFSLLFLSFLSEGDLLAVNG-EKTWCVARPSSDKATLLANINYACSQVDCRIM 60

Query: 110 QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLYP 160
           QKGCPCFYPDNLIN AS+AMNLYY+S+GKNQWNCDFRGS LIVITDPSYGNC+YP
Sbjct: 61  QKGCPCFYPDNLINGASVAMNLYYHSRGKNQWNCDFRGSALIVITDPSYGNCIYP 114

[Close]

BLAST of Cp4.1LG02g02240 vs. TAIR 10
Match: AT1G78520.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 152.5 bits (384), Expect = 2.8e-37
Identity = 68/114 (59.65%), Postives = 81/114 (71.05%), Query Frame = 0


Query: 46  MAKAVAGAVTLSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQVDCRIM 105
           MAKA      L FL L    + + VN   KTWCVA+PSSD   L  NI++ACS VDCR++
Sbjct: 1   MAKAWICLSFLIFLYLVSERNFINVNAETKTWCVAKPSSDQVALQDNINFACSHVDCRVL 60

Query: 106 QKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 160
             GCPC+ P NLINHASIAMNLYY + G+N WNC+F+ SGLI IT+PSYGNC Y
Sbjct: 61  LSGCPCYSPSNLINHASIAMNLYYQANGRNYWNCNFKNSGLITITNPSYGNCYY 114

[Close]

BLAST of Cp4.1LG02g02240 vs. TAIR 10
Match: AT2G43670.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 130.2 bits (326), Expect = 1.5e-30
Identity = 57/117 (48.72%), Postives = 85/117 (72.65%), Query Frame = 0


Query: 46  MAKAVAGAVTLSFLLLFLSGDLLAVNGYQKT---WCVARPSSDHATLLANIDYACSQVDC 105
           MAKA      + FL L+  G+ + V    ++   WCVA+PS+D+  L  NI++ACS++DC
Sbjct: 1   MAKAQICLCFIIFLYLWPEGNFIKVTKADRSAGDWCVAKPSTDNERLQENINFACSKIDC 60

Query: 106 RIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 160
           +I+ +G  C+ PD++I+ AS+AMNLYY ++G++ WNC+F GSGLI ITDPSYG+C+Y
Sbjct: 61  QIISEGGACYLPDSIISRASVAMNLYYQAQGRHFWNCNFEGSGLIGITDPSYGSCIY 117

[Close]

BLAST of Cp4.1LG02g02240 vs. TAIR 10
Match: AT4G09462.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 105.1 bits (261), Expect = 5.2e-23
Identity = 48/105 (45.71%), Postives = 65/105 (61.90%), Query Frame = 0


Query: 56  LSFLLLFLSGDLLAVNGYQKTWCVARPSSDHATLLANIDYACSQ-VDCRIMQKGCPCFYP 115
           L+ L + LS  ++      KTWC+A   + +A L ANI++ACSQ VDCR +Q G  CF P
Sbjct: 8   LALLFIMLSSIMINHVASSKTWCIATLIATNAQLQANINFACSQGVDCRPIQPGGSCFIP 67

Query: 116 DNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 160
           +NL NHAS  MN YY + G+    C F+ +G    TDPS+G C+Y
Sbjct: 68  NNLANHASFVMNSYYQTHGRTNQACSFKNTGTFAATDPSFGKCVY 112

[Close]

BLAST of Cp4.1LG02g02240 vs. TAIR 10
Match: AT3G28250.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 101.3 bits (251), Expect = 7.5e-22
Identity = 41/77 (53.25%), Postives = 56/77 (72.73%), Query Frame = 0


Query: 77  WCVARPSSDHATLLANIDYACSQVDCRIMQKGCPCFYPDNLINHASIAMNLYYNSKGKNQ 136
           WCVA+P +    L+ N++YACS VDC+I+     C+ PDN+ N AS+ MNLYY ++G+N 
Sbjct: 3   WCVAKPGTLTEQLINNLNYACSIVDCQIISTRGACYSPDNIYNMASVVMNLYYQAEGRNF 62

Query: 137 WNCDFRGSGLIVITDPS 154
           WNC+F  SGL+ ITDPS
Sbjct: 63  WNCNFGDSGLVAITDPS 79

[Close]

BLAST of Cp4.1LG02g02240 vs. TAIR 10
Match: AT4G09464.1 (Carbohydrate-binding X8 domain superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 9.7e-22
Identity = 47/105 (44.76%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 60  LLFLSGDLLAVN----GYQKTWCVARPSSDHATLLANIDYACSQ-VDCRIMQKGCPCFYP 119
           LLF+    + +N       KTWC+A   + +A L ANI++ACSQ VDCR ++ G  CF P
Sbjct: 10  LLFIMLSSIMINHIHVASSKTWCIATLIATNAQLQANINFACSQGVDCRPIRPGGSCFIP 69

Query: 120 DNLINHASIAMNLYYNSKGKNQWNCDFRGSGLIVITDPSYGNCLY 160
           +NL NHAS  MN YY + G+    C F+ +G    TDPS+G C+Y
Sbjct: 70  NNLANHASFVMNSYYQTHGRTNQACSFKNTGTFAATDPSFGKCVY 114

The following BLAST results are available for this feature:

BLAST of Cp4.1LG02g02240 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52409	3.4e-19	46.99	Glucan endo-1,3-beta-glucosidase OS=Triticum aestivum OX=4565 GN=GLC1 PE=2 SV=1	[more]
Q94G86	4.4e-19	46.51	Glucan endo-1,3-beta-D-glucosidase OS=Olea europaea OX=4146 GN=OLE9 PE=1 SV=1	[more]
Q84V39	2.9e-18	47.13	Major pollen allergen Ole e 10 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
O65399	6.4e-18	34.23	Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 ...	[more]
Q8VYE5	1.9e-17	43.33	Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360...	[more]

BLAST of Cp4.1LG02g02240 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023525198.1	1.14e-82	100.00	major pollen allergen Ole e 10-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023525199.1	6.13e-80	99.13	major pollen allergen Ole e 10-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022982185.1	5.69e-78	97.32	major pollen allergen Ole e 10-like isoform X1 [Cucurbita maxima]	[more]
XP_022941529.1	3.29e-77	96.43	glucan endo-1,3-beta-glucosidase-like isoform X1 [Cucurbita moschata] >KAG703746...	[more]
KAG6607951.1	6.64e-77	95.54	Glucan endo-1,3-beta-D-glucosidase, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Cp4.1LG02g02240 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J1Y2	2.75e-78	97.32	major pollen allergen Ole e 10-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1FNL6	1.59e-77	96.43	glucan endo-1,3-beta-glucosidase-like isoform X1 OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1IYM5	1.48e-75	96.43	major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1FTX7	8.55e-75	95.54	major pollen allergen Ole e 10-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1CBF4	6.01e-64	82.61	glucan endo-1,3-beta-D-glucosidase OS=Momordica charantia OX=3673 GN=LOC11100915...	[more]

BLAST of Cp4.1LG02g02240 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78520.1	2.8e-37	59.65	Carbohydrate-binding X8 domain superfamily protein	[more]
AT2G43670.1	1.5e-30	48.72	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G09462.1	5.2e-23	45.71	Carbohydrate-binding X8 domain superfamily protein	[more]
AT3G28250.1	7.5e-22	53.25	Carbohydrate-binding X8 domain superfamily protein	[more]
AT4G09464.1	9.7e-22	44.76	Carbohydrate-binding X8 domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 76..159 e-value: 2.1E-38 score: 143.6
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 76..145 e-value: 6.6E-19 score: 68.4
None	No IPR available	GENE3D	1.20.58.1040		coord: 71..157 e-value: 3.0E-10 score: 42.3
None	No IPR available	PANTHER	PTHR31044:SF38	MAJOR POLLEN ALLERGEN OLE E 10-LIKE	coord: 53..160
IPR044788	Carbohydrate-binding X8 domain-containing protein, plant	PANTHER	PTHR31044	BETA-1,3 GLUCANASE	coord: 53..160

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG02g02240.1	Cp4.1LG02g02240.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009506	plasmodesma
molecular_function	GO:0016787	hydrolase activity